Integra MEDIACLAVE 10 User manual

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Quick Start Guide MEDIACLAVE 10/30 Media Sterilizer
This quick start guide is intended to provide a quick overview of your MEDIACLAVE’s
(MC) key features and to offer basic instructions for getting started. For
detailed
information, please refer to the operating instructions (OI)
that can be found at
www.integra-biosciences.com in different languages.
Intended use
This is a general-purpose laboratory instrument. Any use of this instrument in a medical or IVD setting
is the sole responsibility of the user. MEDIACLAVE 10/30 is used for the preparation and sterilization
of media and can easily be converted to a water bath (or for MC10 only to an autoclave for media
sterilization in glassware).
Safety information
Regardless of the listed safety notes, all locally applicable regulations must be observed.
1) This product may only be operated in a secure, protected network with trustworthy clients.
2) MEDIACLAVE may only be used by properly trained personnel in a manner specified by
INTEGRA Biosciences.
3) In error case the instrument must not be used, e.g. if the pressure value is not displayed or above
1.7 bar or hot steam is released from the safety valve. There is the risk of burns or explosion.
The instrument must be immediately switched off and separated from electricity supply. Stay
away from the device.
4) Observe the hazard warnings on the device. Caution is required when opening ports or the vessel
lid (potential risk of delay in boiling). Always wear safety goggles and oven gloves.
5) The hoses may get hot. Do not touch the hoses during operation.
6) Servicing work and repairs may only be performed by INTEGRA Biosciences or an authorized
after-sales service member.
7) It is mandatory that a service is carried out at least once a year or after 1000 sterilization cycles
(whatever is reached first).
Getting started
Install the instrument on a horizontal surface according to the IQ (PN 136951) and OQ (PN
136952) documents.
a. Safety cover
b. Safety valve
c. Dispense port
d. Vessel lid
e. Temperature probe for media
f. Adding port
g. Safety cover lock
h. Operating panel
i. Main switch
j. USB port
k. Printer (optional)
Switch on/off:
Press the main switch.
Switch on the device. In the MAIN MENU, three options are accessible:
•SELECT PROGRAM: To define and execute a program.
•MAINTENANCE: To drain or fill the vessel, automatically clean the
device and perform the safety valve tests.
•SYSTEM PARAMETER: To configure general device parameters,
make a backup to USB, etc..
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d
e
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Language selection
Navigate to SYSTEM PARAMETER and LANGUAGE SELECTION. Select a language and press
SAVE. Set other system parameters to your requirements.
Preparing a media preparation program
•Check, if the white pivot pin disk inside the cuvette is pushed fully downwards and place the
magnetic stirrer bar on the pivot pin inside at the cuvette bottom.
•Place the cuvette in the instrument vessel.
•Using both grips, turn the cuvette approx. 2 cm
clockwise until the bolt locks into position as shown
beside.
•To remove the cuvette, turn it counter clockwise and lift it up.
Fill coupling water
•Prepare softened coupling water: Add a cup of tap water or a pinch
of salt to distilled water to make it conductive, which is required for
the level sensors.
•Navigate to MAINTENANCE, select DRAINING/FILLING and press
FILL COUP. WATER.
•Fill the vessel with coupling water until the upper level sensor is
covered (approx. 2.7 l for MC10, 8.5 l for MC30).
Select a program
•Navigate to SELECT PROGRAM. The first programs are already pre-defined with default values:
Operation mode
Description
STANDARD
For preparation and sterilization of media
CHOCOLATE AGAR
Two step program for preparation of complex media.
AUTOCLAVE
For sterilization of media in glassware (MC10 only)
WATER BATH
For pre-swelling, warming up and thermostatting media
•Select a program to be defined using the arrow keys and press SELECT PROGRAM and
PROGRAM SETTINGS in order to adjust the program.
•Using the arrow keys, select a parameter you wish to change. Press CHANGE and follow the
information on the screen.
Run a STANDARD or CHOCOLATE AGAR program
The nominal volume of the cuvette (10/30L) must not be exceeded. Consider vortex, foam
or bubble formation and swelling.
•Navigate to SELECT PROGRAM. Select the previously defined STANDARD or CHOCOLATE
AGAR program. Add water and ingredients according to your recipe into the cuvette. Press
START and follow the instructions on the screen.
The semi-rigid temperature probe (MC10) must be handled with great care and must not
be deflected more than 30 degrees from the vertical! Do not clamp the cable of the flexible
temperature probe when closing the vessel lid.

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•Close the vessel lid by turning the grips clockwise
around the attachment point. The grips of the
vessel lid (1) must be aligned above the black
stickers (2).
•Close the safety cover and keep it pressed down until it is locked by the bolt after pressing
START.
Dispensing the media
When the dispensing phase is reached, you are informed by an acoustic sound (press to switch
off).
•Press START DISPENSE. The media can now be dispensed through the sterile dispensing
port. For dispensing by an external pump, e.g. DOSE IT or MEDIAJET, press STANDARD
DISPENSE. (For other dispense option please refer to the OI.)
•Unscrew the dispense port cap and insert the sterile fitting for dispensing tubing with silicone
tubing (inner diameter of 6 mm) connected. Connect the tubing to the external pump.
•After dispensing press BACK and press twice END DISPENSE to finish the process. Open the
vessel lid by turning the grips counterclockwise.
Run an AUTOCLAVE program (MC10 only)
When using the AUTOCLAVE mode, the autoclave cuvette and the flexible temperature probe must
be installed, see OI for length adaption.
The MEDIACLAVE is not suitable for sterilization of instruments, glassware and medical
devices. All containers must be opened throughout he whole process.
•Navigate to SELECT PROGRAM. Select the previously defined AUTOCLAVE program and
press START.
•Insert the glassware filled with media into the cuvette and distribute it evenly.
•Fill the vessel with coupling water such that the water level is slightly under the medium surface
inside the glass container for optimal heat transfer.
•Put the flexible temperature probe into a reference container.
•Press START and follow the instructions on the screen.
•Once the autoclave phase is finished press END to open the safety cover. Open the vessel lid.
Run a WATER BATH program
When using the WATER BATH mode, the vessel lid and safety cover must be left opened.
•Navigate to SELECT PROGRAM. Select the previously defined WATER BATH program and
press START.
•For thermostatting of media in glassware (MC10 only), install the autoclave cuvette and insert
the glassware. Fill the vessel with coupling water as described for AUTOCLAVE.
•For prewarming and preswelling of media install the cuvette with stirrer and add the ingredients.
Press START.
•Press END to terminate the thermostatting phase.

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Maintenance
The MEDIACLAVE requires periodical cleaning in order to ensure safe and reliable
operation. Daily and monthly cleaning procedures according to the OI are mandatory.
Before starting manual cleaning, ensure the device is switched off and disconnected form
the electricity supply.
Daily:
•Unscrew the dispensing tubing, disconnect the decanting tubing on the underside of the vessel
lid and rinse the tubings.
•At the end of a working day, navigate to MAINTENANCE, select DRAINING/FILLING and press
DRAIN COUP. WATER to empty the vessel automatically.
•Clean the following instruments parts with a lint free cloth and washing detergent:
cuvette; magnetic stirrer; pivot pin; temperature probe; vessel including the coupling water level
sensors, deaeration opening and drain; vessel lid with dispense and adding ports, safety valve;
lid seal; housing. Do not let solution drip inside the device.
•If you use media with salt concentrations above 3 %, causing corrosions of stainless steel, rinse
all salt residues thoroughly with plenty of water. Apply a chrome steel cleaning agent to all
affected surfaces according to the instructions by the manufacturer. Completely remove it with
warm water and a sponge.
•Dry all areas with paper towels.
Monthly:
•Navigate to MAINTENANCE, select CLEANING and follow the instructions on the screen.
•Navigate to MAINTENANCE, select SAFETY VALVE. Insert the cuvette, fill the vessel with
coupling water and follow the instructions on the screen.
•Perform Daily cleaning including check of steel surfaces.
•Check the white pivot pin disk below the stirrer. It must be replaced if its thickness is below
1 mm.
Equipment Disposal
MEDIACLAVE must not be disposed of with unsorted municipal waste.
Dispose of MEDIACLAVE
in accordance with the regulations in your area governing
disposal of devices.
Manufacturer
INTEGRA Biosciences AG
CH-7205 Zizers, Switzerland
T [phone]
F [phone]
INTEGRA Biosciences Corp.
Hudson, NH 03051, USA
T [phone]
F [phone]
[e-mail]
www.integra-biosciences.com

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