Integra VIAFLO 96 User manual

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Quick Start Guide VIAFLO 96/38424/96/384ChannelPipette
This quick start guide is intended to provide a quick overview of your
VIAFLO 96/384’s key features and to offer basic instructions for getting started. For
detailed information, please refer to the operating instructions (OI) that can be found
at www.integra-biosciences.com in different languages.
Intended use
This is a general-purpose laboratory instrument. Any use of this instrument in a medical or IVD
setting is the sole responsibility of the user. VIAFLO 96 and VIAFLO 384 are electronic hand held
pipettes that can be used with 24-, 96- and 384-channel (VIAFLO 384 only) pipetting heads for
aspirating and dispensingaqueous solutions in the volumerange of 0.5 μl to 1250 μl usingGripTip™
pipette tips only, see www.integra-biosciences.com.
Safetyinformation
Regardless of the listed safety notes, all locally applicable regulations must be observed.
1) The pipette may only be usedby properly trained personnel in a manner specified by INTEGRA
Biosciences.
2) Do not use the instrumentnear flammablematerial or in an atmospherewith danger of
explosion.
3) Do not immersethe pipetting head in liquid. Avoid pipetting of liquids emitting corrosive vapors.
4) Servicing work andrepairs may only be performed by INTEGRA Biosciences or an authorized
after-sales service member.
Gettingstarted
Set up the instrument on a perfectly horizontal surfaceaccording to the IQ/OQ document
(PN 125953). Supply voltage: 100 zH06 – 05,CAV042 – .
Insert a 3 core power cable in the socket on the rear side and connect it to the electricity mains.
a. Carrying handle of base unit
b. Pipetting unit, to move up and down (Z-axis)
c. Knob of Side cover, covers pipetting head
d. Base unit, to move left and right (X-axis)
e. Main switchand power socket
f. Plate slider
g. Plate holders on position A and B
h. Instrument deck
i. Control unit
j. Tip load button
Switch on/off:
Press the main switch(e).
VIAFLO 96/384 control unit
k. Display
l. Touch wheel, spin to scrollthe cursor
m. OK button, to make a selection
n. Run key, to start operations
o. Tip ejector
p. Purge button, to empty tips
q. Arrow buttons, for selections
r. Back button, to navigate backward
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Change Head
To install a pipetting head, select the Toolbox option “Change Head”. Remove pipetting head from
case and pull Piston plate (1) up.
Loosen the knob (c) and remove the Side cover.
Remove the existing head, if installed.
Pushthe appropriate pipetting head into the slide block and make surethe
Piston plate (1) slides onto the brazen rails.
Mount the Side cover, screw the knob tightly and press OK to continue.
Check whether the Pipet factor on the display corresponds to the Pipet factor indicated in the latest
calibration certificate. Press OK if the factor coincides. If it doesn’t, click Edit.
Loading GripTips
Put a tip box on the Plate holder.
Hold the Control unit and lower the pipetting head down onto the tip box
until the Tip load button flashes.
Whenprompted,pushthe Tip load button and at the sametimepushdown
the Control unit. The Tip load button lights after loading.
Move the Pipetting unit up until the light of the Tip load button turns off.
If less than the full tip rack needs to be loaded, first press OK before pressing
the Tipload button to switchto thelowpower loading mode.Enter thenumber
of columns to be loaded.
Selectinga pipettingmode
With your electronic pipette, you have the choice of building protocols by selecting from a menu of
predefined programmingmodes,or you cancreate custom multi-steppedprotocols. A description of
the most commonly used Pipetting Modes:
Pipettingmode
Description
Pipet
Liquid transfers when aspirate and dispense volumes are equal.
Repeat Dispense
Dispensemultiple aliquots of the samevolume.
Sample Dilute
Aspirate two liquids divided by an air gap, followed by dispense.
Pipet/Mix
Multiple mixing by aspiration and dispensing of defined volume.
Manual Pipet
Control the aspiration and dispensing up to the setvolume.
Reverse Pipet
Liquid transfers of viscous or high vapor pressure liquids.
Variable Dispense
Dispensemultiple aliquots of different volumes.
Multi Aspirate
Aspirate multiple aliquots of different volumes.
Sample Dilute/Mix
Aspirate two liquids divided by an air gap, followed by dispenseand mix.
Serial Dilution
Aspirate a transfer volume followed by dispense and mix sequences.
Custom
Allows to create and store of up to 40 multi-stepped pipetting protocols.
From the Main Menu, use the Touch Wheelto scroll to your desired function-
based programming mode. Press OKto enter the mode and to start defining
parameters.
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Setting/changingparameters
Scroll to Edit and
press OK.
A list of editable steps is
displayed.
Select a step and press OK.
Use the Touch Wheelto set the
value and press OK.
Press to save your settings.
Running a program
Insert the GripTips into the liquid to be transferred. Press and release the Run key to aspirate the
volume selected in the first step (marked with a triangle on the Run screen).
Your pipette will prompt you to press the Run key at each successive step.
Set Z-Position
To define the optimal tip immersion depth, press or to access the Z-
position screen.
Move the pipetting unit to the target position (A or B) until the actual Z-
height is displayed.
Finally, movethe unit downto the desireddispensing height and selectSet
to save your setting.
Twostep blowout
Note: During the last dispense of a program, you can perform a two step blowout to prevent liquid
from being aspirated back into the GripTips:
Press and hold the Run Key to dispense without blowin. If necessary, perform a touch-off.
Remove the GripTips from the target vessel.
Release the Run Key to start blowin.
Using the Plate slider,e.g. forreformatting
To access a plate that has 4x more wells than the head channels, the plate must be shifted beneath
the pipetting head. Toaccommodatethefront orrear positions,setthe plate slider.To accommodate
the left and then the right positions, move the pipetting head one well to the right.
Plate slider at the back: pipette into the front wells.
Plate slider in the middle: pipette into plates that have as many
wells as the head has channels.
Plate slider at the front: pipette into the rear wells.
Maintenance
Always switch off the device and disconnected form the electricity supply when carrying
out maintenance work.
Clean the VIAFLO 96/384 housing a lint-free cloth lightly soaked with mild soap solution in
distilled water or with a 70 % solution of isopropanol or ethanol.

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Equipment disposal
VIAFLO 96/384 mustnot be disposed of with unsorted municipal waste.
Disposeof VIAFLO 96/384 in accordancewith the regulations in your area governing
disposal of devices.
Manufacturer
INTEGRABiosciencesAG
CH-7205 Zizers, Switzerland
T [phone]
F [phone]
[e-mail]
www.integra-biosciences.com
INTEGRABiosciencesCorp.
Hudson, NH 03051, USA
T [phone]
F [phone]