Labnet BioPette E BE20-1 series User manual

BioPette E
Single and 8-channel Electronic Pipettes
Operation Manual
Version: 2.7
www.labnetlink.com

Labnet InternationalLabnet International
PO Box 841PO Box 841
Woodbridge, NJ 07095Woodbridge, NJ 07095
USAUSA
Declaration of ConformityDeclaration of Conformity
Product Name: BioPette E Single/8-channel Electronic PipettesProduct Name: BioPette E Single/8-channel Electronic Pipettes
Model Names: BE20-1, BE200-1, BE1000-1, BE20-8, BE 200-8Model Names: BE20-1, BE200-1, BE1000-1, BE20-8, BE 200-8
All models comply with the following European standards:All models comply with the following European standards:
EMC: EN 55011, Class AEMC: EN 55011, Class A
EN 60601-1-2EN 60601-1-2
To the best of my knowledge and belief, these units conform to these
standards.
To the best of my knowledge and belief, these units conform to these
standards.
Name: Mike ReveszName: Mike Revesz
Position: Director of Quality ControlPosition: Director of Quality Control
Issue Date: 2003/4/30Issue Date: 2003/4/30
Copyright Notice:Copyright Notice:
N
o part of this manual may be reproduced or transmitted in any
form or by any means, electronic or mechanical, for any purpose,
without prior written permission of Labnet International, Inc.
N
o part of this manual may be reproduced or transmitted in any
form or by any means, electronic or mechanical, for any purpose,
without prior written permission of Labnet International, Inc.

Table of Contents
1. Safety Precautions...................................................................................1
1.1. Operation Safety Precautions ..........................................................1
1.2. Battery Safety..................................................................................1
2. General Description................................................................................3
2.1. Features............................................................................................3
2.2. Ranges and Specifications...............................................................4
3. Getting Started ........................................................................................4
3.1. Unpacking........................................................................................4
3.2. Inserting the Battery ........................................................................5
3.3. Charging the Battery........................................................................6
3.4. Start Pipetting..................................................................................8
3.5. Hanger Installation ..........................................................................9
4. BioPette E Overview...............................................................................9
4.1. Keypad Function............................................................................13
4.2. Audible Signals .............................................................................13
5. Function Map ........................................................................................15
6. SYS (System) Mode...............................................................................16
6.1. Aspirating Speed............................................................................16
6.2. Dispensing Speed ..........................................................................17
6.3. Audible Signals .............................................................................17
6.4. Tip Ejector Alarm..........................................................................17
6.5. Blow-out........................................................................................18
6.6. Reverse Pipetting...........................................................................18
6.7. Setting PROG (Programs) .............................................................19
6. AUTO Mode 20

7. MD (Multiple Dispensing) Mode .........................................................20
8. MIX Mode 21
9. SE (Sequential Aspirating/Dispensing) Mode.....................................22
10.1. SA (Sequential Aspirating) Mode – Not available on BE1000-1
because the diameter of 1000ul tips does not allow the required air gap to be
maintained...................................................................................................22
10.2. SD (Sequential Dispensing) Mode ................................................24
10. Sterilization 26
11.1. Removal of the Lower Piston Assembly .......................................27
11.2. Reattach the lower piston assembly...............................................28
11. Maintenance 29
12. Troubleshooting.....................................................................................30
Appendix A: Technical Specifications...........................................................32
Appendix B : Tip Ejector Height Adjustment..............................................32
Appendix C: Warranty...................................................................................33

1. Safety Precautions
Before using the BioPette E for the first time, please read this entire
operating manual carefully. To guarantee problem free, safe operation of the
BioPette E, it is essential to observe the following points:
1.1. Operation Safety Precautions
When using infectious, radioactive, toxic and other solutions which
may pose health risks, please observe appropriate safety precautions.
1. Do not use the BioPette E in a potentially explosive environment or
with potentially explosive chemicals.
2. When using organic solvents or corrosive chemicals, please check their
compatibility with the pipette tips and the BioPette E.
3. Repair should be carried out by Labnet authorized service personnel
only. Contact Labnet International, Inc. for information on authorized
service options.
4. Use original spare parts and accessories only.
1.2. Battery Safety
The BioPette E operates on a small but powerful Lithium-ion battery.
Misuse or abuse of the Lithium-ion battery may cause damage or injury
through fire, electric shock, or chemical leakage. Please read and
understand all warnings before using the battery.
1. When storing the battery, do not allow it to come into contact with any
metallic surfaces.
2. Never use the BioPette E while using the DC-in jack to charge the
battery.
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3. Do not incinerate the Lithium battery or expose it to excessive heat.
4. Do not short-circuit, puncture, crush, disassemble, damage, force
over-discharge (reversal) or modify the battery.
5. Do not expose the battery to water or moisture.
6. Do not drop or subject the battery to strong impacts.
7. Only use the battery specified in this BioPette E manual.
8. Only use the specified, battery charging unit or DC-in jack to charge
the battery.
9. Do not use a leaking battery.
10. If charging is not completed within the specified time period, unplug
the charger and discontinue charging immediately.
11. The charger and battery temperature rises with extended periods of use.
Care should be taken to avoid burns.
12. Burns may result if the battery is removed immediately after extended
periods of use.
13. If fluid from the battery enters your eye, immediately rinse the eye with
plenty of fresh water and contact a doctor. If fluid from the battery
makes contact with your skin or clothing, wash the area thoroughly
with water.

2. General Description
Labnet's BioPette E is designed to replace the traditional manual pipette. It
features an accurate and efficient, as well as ergonomic and light-weight
design. Researchers can program this state-of-the-art product to conduct
different pipetting tasks. More accurate and comfortable pipetting can be
easily achieved without any concern of finger stress or injury.
2.1. Features
zHigh accuracy and precision
zLow forces
zUseful, practical operation protocols, such as Automatic
Pipetting(AUTO), Multiple Dispensing(MD), Mixing(MIX), and
Sequential Aspirating/Dispensing (SE), etc.
z9 user-defined program sets
z5 speeds for aspirating and dispensing
zLi-ion battery provides extended use on a single battery charge
zErgonomic design
zAutomatic calibration
zUser-friendly graphic operation interface.
zAdjustable tip ejector.
zAutomatically remembers the last-used pipetting protocol and settings
zComplies with CE, ISO-8655, GLP
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2.2. Ranges and Specifications
Product No. Channels Volume
Range (µl)
Increment
(µl)
Accuracy
(Rel.±)
Precision
( Rel. CV )
BE20-1-X 1 2 -20 0.1
7.0-1.0% 2.0-0.3%
BE200-1-X 1 10 -200 1 2.5-0.8% 1.0-0.15%
BE1000-1-X 1 100 -1,000 1 3.0-0.8% 0.6-0.15%
BE20-8-X 8 2-20 0.1 7.0-1.0% 2.0-0.3%
BE200-8-X 8 10-200 1 3.5-0.8% 1.0-0.15%
Note:
1. X: power plug, no designation=U.S. plug, EU=European plug,
UK=U.K. plug, AU=Australian plug
2. BioPette E Accuracy and Precision Specifications have been attained
using Labnet brand pipette tips. Labnet guarantees Accuracy and
Precision only if Labnet brand tips or other approved tips are used.
3. Getting Started
3.1. Unpacking
Open the BioPette E package and confirm that all items are included:
zSingle/8-channel BioPette E unit zOperation manual
zQuality assurance card zAC-DC power adaptor
zBioPette E hanger and
accessories zPipette tips
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If there are any items missing, damaged, or not according to your order,
please contact your distributor or sales representatives for replacement
immediately.
Please charge the battery before first-time operation.
3.2. Inserting the Battery
Figure 1: Open the battery cover. Figure 2: Insert the battery.
Remove the battery cover by pressing the latch (see Figure 1). Insert the
battery (see Figure 2). Metallic contacts must be face down. Close the cover
of the battery compartment. Press the RESET button to power on and
calibrate the BioPette E.
Note: After a charged battery is inserted, the BioPette E will automatically
carry out a calibration routine.
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3.3. Charging the Battery
BioPette E’s Internal Charging Circuit
Optional Charger
There are two ways to charge the battery: (1) Use BioPette E’s internal
charging circuit, (2) Use the optional charger.
Insert the battery into the battery compartment. Insert the DC-in plug of the
power adaptor into the DC-in jack (see Figure 3). Connect the power plug
of the power adaptor with the external power source. Connecting the power
plug before inserting the battery will inactivate the charging process. The
indication bar inside battery symbol will blink during the charging
process. When the battery is fully charged, the blinking will stop and the
BioPette E will beep once to remind the user.
Connect the DC-in plug of the AC-DC power adaptor to the charger.
Connect the power plug of the AC-DC power adaptor to the external power
source. The indication light will turn green. Insert the battery (see Figure 4).
Once the battery is correctly inserted and charging, the indicator light will
turn red. When the battery capacity is full, the indicator light will turn green
again.
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Figure 3: Using BioPette E’s internal
charging circuit to charge the battery Figure 4: Using the optional charger to charge the battery
Note:
1. For safety reasons, the BioPette E cannot be used during charging.
2. Please do not over-charge the battery for a long period of time.
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3.4. Start Pipetting
Figure 5: Correct hand position
There is no power On/Off button on the BioPette E. Once users insert the
fully-charged battery, the BioPette E will be switched on. It will perform a
calibration routine and switch on the LCD display as in Figure 8. Press any
keypad button or the PLUNGER button to enter the function mode selection.
The BioPette E will be automatically powered off if it’s not in use for more
than 10 minutes.
Select the desired function mode and settings (refer to the following
chapters : 6 ~ 10 ) before pipetting. After the initial set-up, attach the
correct-sized tips to the cone(s) of BioPette E before operation.
The BioPette E is an ergonomic instrument designed to reduce hand fatigue
and repetitive stress injury. In order to maximize the benefits of the
BioPette E’s design, the instrument should be held as shown in figure 5.
Use your index finger to press the PLUNGER button and use your thumb to
press the TIP ejector.
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3.5. Hanger Installation
The BioPette E hanger can be attached to a shelf or counter using the screw
or sticker as shown in Figure 6. The finger rest of the BioPette E locks into
the hanger and keeps the BioPette E in a vertical position.
Figure 6: BioPette E hanger installation
4. BioPette E Overview
This section presents an overview of the BioPette E’s various components
and buttons (see Figure 7) as well as the symbols and indicators on the
LCD display (see Figure 8).
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LCD Display
10
Figure 7: 1-channel BioPette E overview
Keypad
Tip Ejector
Bushing
Lower Part
Cone
Battery
C
"RESET
"
"PLUNG
Finger R
Connect
i
(Front View) (Side View)
Figure 1 : Overview

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Figure 8: LCD display Panel
4.1. Keypad Function
Button Symbol Function Description
Set/Enter Confirms your choice
Exit/Esc Exits to previous mode/setting
Up Increases volume/ changes parameter
Down Decreases volume/ changes parameter
Reset RESET Piston automatically determines the
home position
PLUNGER PLUNGER Activates aspirating/dispensing/
same as button
Tip Ejector TIP Ejects the tips
Tips for Efficient Operation: The PLUNGER button can be used to increase
volumes and to change modes and settings same as the “Up” button during
the function mode selection and volume settings. This operation feature can
speed up the setting processes.
4.2. Audible Signals
The audible signals are beneficial when you are familiarizing yourself with
the operating procedure of the BioPette E. They can also be switched off if
desired (see Section 6.3.)
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Audible Sound Operation
Low-tone beep Aspirating
High-tone beep Dispensing/ Tip Ejection/ Error codes
2 beeps MD/MIX/SE mode cycle or RESET routine is
completed

5. Function Map
The main menu of the BioPette E includes five function modes (SYS,
AUTO, MD, MIX and SE). Each mode includes various function settings
(see Figure 9).
SYS AUTO MD MIX SE
Function
Mode
Aspirating
Speed Dispensing
Number
(COUNT)
Basic
Aspirating/
Dispensing
Mix
Number
(
COUNT
)
Dispensing
Speed
Audible
Signals
Program
Recall
Reverse
Pipetting
Tip Ejection
Alarm
Blow-out
Dispensing
Volume Mix Volume
SDSA
Aspirating
Number
(1,2,3,…)
Dispensing
Number
(1,2,3,…)
Aspirating
Volume
Dispensing
Volume
Function
Settings
Function Map
Figure 9: Function map
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