Labnet Labpette FX User manual

Labnet Labpette FX
Single-channel, Fixed
Volume Pipettors
Instruction Manual
This manual is available in additional languages
at www.labnetlink.com.

Table of Contents
1. Product Description ...................................................................1
2. Packing..............................................................................................2
3. Pipettor Design.............................................................................2
4. Safety Recommendations ......................................................3
 5.Specications ................................................................................4
6. Pipettor Operation ......................................................................5
7. Operating Instructions .............................................................6
8. Aspiration and Dispensing Instructions...........................7
9. Checking Pipetting Accuracy Parameters
and Pipettor Recalibration.......................................................8
10. Pipettor Maintenance ........................................................... 12
11. Troubleshooting ....................................................................... 13
12. Spare Parts................................................................................... 15
13. Limited Warranty...................................................................... 17

Instruction Manual – 1
1. Product Description
LabnetLabpetteFXsingle-channel,xedvolumepipettoris
a volumetric instrument designed to measure and transfer
liquids precisely and safely. It can accommodate volumes
from 2 μL to 1,000 μL depending on the model.
Nominal Volume (µL) Cat. No. Color Code
2P3900-2 Red
5P3900-5 Red
10 P3900-10 Red
20 P3900-20 Yellow
40 P3900-40 Yellow
50 P3900-50 Yellow
70 P3900-70 Yellow
75 P3900-75 Yellow
100 P3900-100 Yellow
120 P3900-120 Yellow
150 P3900-150 Yellow
200 P3900-200 Yellow
250 P3900-250 Green
300 P3900-300 Green
400 P3900-400 Green
500 P3900-500 Green
1,000 P3900-1000 Green
Labnet pipettors operate using an air-cushion (i.e., the
aspirated liquid does not come into contact with the shaft
or plunger of the pipettor). The liquid is drawn into the
disposable tip attached to the pipettor.

2 – LABNET LABPETTE FX PIPETTORS
2. Packing
The pipettors are delivered with the following:
Description Qty/Pk
Instruction manual 1
Qualitycontrolcerticate 1
Calibration key 1
Shelf clip 1
Lubricant 1
3. Pipettor Design
Model Identication
The volume of the pipettor is shown on its pushbutton.
Pipetting pushbutton (A)
Ejector pushbutton (N)
Handle (O)
Tip ejector (D)
Shaft (C)

Instruction Manual – 3
4. Safety Recommendations
Long-term use of the pipettor relies on correct method of use.
Please read and follow the instructions for use carefully.
Symbols used:
Danger, risk of injury.
NOTE Risk of damage to the pipettor or errors in pipetting.
NOTE:
• The pipettor is designed for the transfer of liquids only
using the tip. Do not aspirate liquids without the tip
attached. The aspirated liquid should not enter the
pipettor, as it may cause damage.
• Single-use tips reduce the risk of contamination of
samples.
• Keep the pipettor clean, avoiding the use of abrasive or
corrosive cleaning agents (e.g., acetone).
• Keep the pipettor upright when there is liquid in the tip.
• Only using the pipettor in accordance with the
manufacturer’s instructions ensures the correct pipettor
parameters are maintained.
• After replacing the plunger or the shaft, the pipettor
should be calibrated.
• In the case of incorrect operation, the device should
be cleaned in accordance with the Instructions for Use
or transferred to a service point.
• Ambient operating temperature is +5°C to 45°C.
• Ambient storage conditions (in the original packaging
during transport and short storage) is -25°C to 55°C.
When working with the pipettor:
• Follow general work safety regulations regarding hazards
related to work in the laboratory.
• Take special care when pipetting aggressive substances.
• Use appropriate protective attire (e.g., clothing, goggles,
and gloves).
• Avoid pointing the pipettor at yourself or others during use.
• Only use parts and accessories recommended by the
manufacturer.

4 – LABNET LABPETTE FX PIPETTORS
5. Specications
The pipettor is a high quality instrument which offers
excellent accuracy and precision. The accuracy and precision
(repeatability) of the liquid volume depends on the quality
of pipet tips used. The values for accuracy and precision
shown in the table below were obtained using manufacturer
non-lterpipettips.Thosetipsarerecommendedforuseto
ensure compatibility, accuracy and precision when pipetting.
Nominal
Volume (µL) Cat. No.
Accuracy
(%)
Precision
(%)
Non-lter
Tips (µL)
2P3900-2 ±3.0 ≤1.5 200
5P3900-5 ±2.0 ≤1.0 200
10 P3900-10 ±1.2 ≤0.5 200
20 P3900-20 ±0.9 ≤0.4 200
40 P3900-40 ±0.9 ≤0.3 200
50 P3900-50 ±0.9 ≤0.3 200
70 P3900-70 ±0.9 ≤0.3 200
75 P3900-75 ±0.9 ≤0.3 200
100 P3900-100 ±0.9 ≤0.3 200
120 P3900-120 ±0.8 ≤0.3 200
150 P3900-150 ±0.7 ≤0.3 200
200 P3900-200 ±0.6 ≤0.2 200
250 P3900-250 ±0.6 ≤0.2 1,000
300 P3900-300 ±0.6 ≤0.2 1,000
400 P3900-400 ±0.6 ≤0.2 1,000
500 P3900-500 ±0.6 ≤0.2 1,000
1,000 P3900-1000 ±0.6 ≤0.2 1,000
The accuracy and precision were obtained gravimetrically
using manufacturer tips performing at least 10 measurements
of distilled water at a temperature of 20°C ± 1°C according to
EN ISO 8655 standards.
Theuseoftipsfromothermanufacturersorltertipsmay
result in incorrect liquid aspiration and require pipettor
recalibration.
The design of the pipettor enables the user to recalibrate it
according to the information presented in Section 9.

Instruction Manual – 5
6. Pipettor Operation
Attaching the Tips
• Attach the correct tip corresponding to the
model number displayed on the pipettor
ngerrest(Section5).
• Position the pipettor vertically when
attaching tips.
• Press the pipettor against the tip positioned
in the rack box. The suspension system
will ensure even and airtight sealing of the
pipet tips.
• Hold down the tip ejector button in order to
ensureatightertofthetip.
NOTE:
• Do not attach the tips with a rocking movement, as this
may damage the shaft or plunger.
• Never draw liquids directly into the pipettor without the
tip attached.
Ejecting the Tip
• Place your thumb against the ejector
pushbutton side and press it to the side.

6 – LABNET LABPETTE FX PIPETTORS
7. Operating Instructions
Observing the following recommendations will ensure
maximum possible accuracy and precision of liquid sampling.
• During operation, the volume setting should be locked,
with black adjustment knob in the lower position.
• Ensure smooth and slow operation of the pipettor.
• Immersion of the tip into the sample liquid should be
kept to a minimum depth, which should remain constant
during aspiration. The recommended immersion depths
are given in the table below:
Model Volume Range (µL) Immersion Depth (mm)
0.1-1 ≤1
1-100 2-3
101-1,000 2-4
• The pipettor should be held in a vertical position.
• The pipet tip should be changed whenever the volume
setting is altered, and when a different liquid is to be
aspirated.
• The pipet tip should be changed if a droplet remains on
the end of the tip from the previous pipetting operation.
• Each new pipet tip should be pre-rinsed with the liquid to
be pipetted.
• Liquid should never enter the pipettor shaft. To prevent this:
– Press and release the pushbutton slowly and smoothly.
– Never turn the pipettor upside down.
– Never lay the pipettor on its side when there is liquid in
the tip.
• Never force the volume setting beyond the recommended
limits.
• When pipetting liquids with a different temperature from
the ambient temperature, it is recommended to pre-rinse
the tip several times prior to use.
• Do not pipettor liquids with temperatures above 70°C.
NOTE: When pipetting acids or corrosive solutions which
emit vapors, it is recommended to disassemble the shaft
and rinse the plunger and O-ring with distilled water after
nishingthepipettingoperation.

Instruction Manual – 7
8. Aspiration and Dispensing Instructions
1. 2. 3. 5.4. 6.
Aspirating Liquid
1. Pressthepushbuttontotherststoppoint.
Holding the pipettor vertically, immerse the pipet tip
into the sample liquid to the recommended depth (for
recommended values see Section 7). If the pipet tip
is not immersed to the recommended depth or if the
pipetting pushbutton is rapidly released, air may enter
the pipet tip.
2. Release the pipetting pushbutton slowly and smoothly to
aspirate the sample. Wait one second and then withdraw
the pipet tip from the liquid.
CAUTION: Do not touch the used tip.
Dispensing Liquid
3. Place the end of the pipet tip against the inside wall of
the vessel at an angle of 10° to 40°. Press the pushbutton
smoothlytotherststop.Waitonesecond.
4. Press the pushbutton to the second stop to expel
any remaining liquid. While keeping the pushbutton
depressed, remove the pipettor from the vessel by
drawing the pipet tip against the inside surface of
the vessel.
5. Release the pushbutton to its starting position.
6. Eject the pipet tip by pressing the tip ejector pushbutton
to the side (left or right).
Remember to change the pipet tip whenever a different
type of liquid is to be sampled.

8 – LABNET LABPETTE FX PIPETTORS
Aspirating High-density Liquids
When pipetting liquids of higher viscosity or lower surface
tensionthanwater(e.g.,seraororganicsolvents),almof
liquid may be formed on the inside of the pipet tip which
mayproduceerroneousresults.Asthelmremainsrelatively
constant in successive pipetting operations with the same
tip, this error can be eliminated by pre-rinsing the tip and
allowingalmtoformbeforetransferringtherstsample.
This is achieved by aspirating a sample and dispensing it
backintothesamevessel.Allowingalmtoformpriorto
sampling ensures optimal accuracy and repeatability.
This pre-rinsing operation should be repeated when
the volume to be aspirated is changed or when a new pipet
tip is used.
NOTE: Normally the degree of error resulting from viscous
liquids is negligible if pipetting is performed slowly and
carefully, however can be minimized further by holding the
pipet tip in position for at least 2 seconds after aspiration
to allow the liquid time to react to the change in pressure
before it is dispensed.
If the above method does not result in accurate values,
recalibrate the pipettor in accordance with Section 9.
It is recommended to record recalibration and correction
values, in order to facilitate reverse calibration to a standard
liquid.
9. Checking Pipetting Accuracy Parameters
and Pipettor Recalibration
The pipettors have been factory-calibrated using gravimetric
methods with manufacturer pipet tips and distilled water,
in accordance with ISO 8655 guidelines
for the liquid volume drawn by the pipettor according to
the values given (Section 5). The pipettors are designed to
enable recalibration and adaptation to different pipetting
techniques and liquid properties (e.g., temperature, density,
and viscosity). Periodic checks of the operation of the
pipettor are recommended at least once per year. Frequency
of checks should be increased depending on workload,
sterilization or autoclave processes, and frequency of
replacement of component parts.
If during pipettor operation the accuracy error exceeds the
permissible value given in the table in Section 5, pipettor
recalibration should be carried out. Recalibration of the
pipettor involves adjusting the settings according to the
table on page 10.

Instruction Manual – 9
Parameters for Checking the Pipetting Accuracy
Thepipettingaccuracyisinuencedbyfactorssuchas:
tips used, characteristics of the pipetted liquid (density,
viscosity), and operating conditions (ambient temperature,
pressure).
To determine the accuracy error of the pipettor, the following
conditions should be met:
• Ambient temperature and temperature of the pipettor,
pipet tips, and liquid should be within the range of 20°C
to 25°C and stabilized during weighing within ±0.5°C.
• Measurements should be conducted using distilled water.
• Balance sensitivity should be suitable for the volume “V”
to be measured.
Volume Checked (V, µL) Balance Sensitivity (mg)
0.1≤V≤10 0.001
10≤V≤100 0.01
100≤V≤1,000 0.1
• When calculating the liquid volume aspirated by
the pipettor, the conversion factor (Z) [μL/mg] for distilled
water or a liquid with comparable density should be
taken into account. Sample values of conversion factors
are given in the following table.
Temperature
(°C)
Pressure (kPa)
95.0 101.3 105.0
20 1.0028 1.0029 1.0029
21 1.0030 1.0031 1.0031
22 1.0032 1.0033 1.0033
23 1.0034 1.0035 1.0036
24 1.0037 1.0038 1.0038
25 1.0039 1.0040 1.0040
See ISO 8655 for the full table of conversion factors (Z).
NOTE: Pipetting should be performed in accordance with
guidance described in Sections 7 and 8.

10 – LABNET LABPETTE FX PIPETTORS
Checking the Pipetting Accuracy Parameters
• Set the preset volume depending on the pipettor volume
according to the following table.
Nominal
volume
(µL)
Permissible values
(µL)
Volume Change ΔV (μL) for a Turn
of a Calibration Key by
1 Turn 1 Increment
21.933 - 2.053 0.7 0.03
54.833 - 5.082
10 9.846 - 10.085 1.3 0.05
20 19.751 - 20.110 1.7 0.07
40 39.502 - 40.219 3.5 0.15
50 49.377 - 50.274
70 69.128 - 70.384
7.0 0.3075 74.066 - 75.411
100 98.85 - 100.45
120 118.62 - 120.54
13.0 0.54
150 148.43 - 150.52
200 198.11 - 200.50
250 247.63 - 250.62
31.0 1.3
300 297.16 - 300.75
400 396.21 - 401.00
500 495.27 - 501.25
1,000 990.53 - 1,002.49 60.0 2.5
• Perform 10 aspirations, and calculate the average value
in [mg].
• Calculate the volume in [μL] by multiplying the value
in [mg] by the conversion factor Z [μL/mg].
If the average aspirated volume exceeds the permissible
values of the range, the pipettor should be recalibrated.

Instruction Manual – 11
Pipettor Recalibration
K
1. Remove the pipetting pushbutton.
2. Insert the calibration key into the calibration screw.
3. Turn the key clockwise to reduce the aspirated volume, or
counter-clockwise to increase the aspirated volume Use
the values given in the above table to precisely adjust the
volume.
4. Remove the key and replace the pipetting pushbutton.
Determine the average aspirated volume. The average
volume should be within the permissible range given in
the table. If the volume exceeds the values stated, the
recalibration procedure should be repeated.
When pipetting liquids with physical properties
considerably different from those of water, follow
the guidance given in Section 5.
More information on the calibration procedure can be found
at www.labnetlink.com.

12 – LABNET LABPETTE FX PIPETTORS
10. Pipettor Maintenance
Depending on the applications and intensity of use, the
pipettor requires periodic maintenance. The components
exposed to corrosive vapors, such as shaft elements, should
be regularly checked and cleaned. Do not use sharp tools for
pipettor maintenance. It may cause damage to the device
and affect the user’s safety.
Cleaning
External surfaces of the pipettor such as the pushbutton,
ejector pushbutton and handgrip, may be cleaned using a
cloth dampened in isopropyl alcohol. The remaining parts
removed from the pipettor during pipettor disassembly may
be washed with distilled water or isopropyl alcohol.
NOTE: Before using cleaning agents other than those
recommended by the manufacturer, check the compatibility
charts and consider chemical resistance of the following
plastics which form components of the pipettor: PP, PC,
POM, PA, PPS, PVDF.
Sterilization
Sterilization using an autoclave
Sterilization using an autoclave The pipettor can be
sterilized in an autoclave at 121°C for 20 minutes.
Sterilization under other conditions may cause damage to
the pipettor. It is recommended to:
• Sterilize the pipettors using an autoclave with an initial
vacuum and drying cycle.
• After sterilization, the pipettor should be dried and cooled
to room temperature.
Precision and accuracy should not alter if the pipetting
processes including autoclaving are carried out as
described in this manual. If a change in accuracy occurs, it is
recommended to:
• Checkthecalibrationofthepipettoraftertherst,
third,andfthautoclavingcyclesandthenafterevery
10 autoclaving cycles.
Ultra Violet (UV) Sterilization
The pipettors are UV resistant. The distance from the
radiation source to the exposed element of the pipettor
should be at least 50 cm. Prolonged or intense UV exposure
can cause discoloration of pipettor parts but does not affect
its performance.

Instruction Manual – 13
11. Troubleshooting
If a problem is encountered during pipettor operation,
use the following table to identify and eliminate the fault
following the instructions provided. Replacement of parts
should be required only occasionally and should not be
required with normal pipettor use.
Problem Cause Solution
Droplets of
liquid remain
in the pipet
tip.
The pipet tip is emptied
too fast.
Decrease the speed
of pressing the
pipettor pushbutton.
The pipet tip wettability
has increased due to
extensive use.
Replace the tip with
a new one.
Droplets
of air appear
in the liquid
aspirated
into the pipet
tip.
The pipet tip immersion
depth is too shallow.
Immerse the tip
to the recommended
depth according to the
instructions.
The pipet tip is incorrectly
pressed onto the pipettor
shaft.
Press the pipettor
tiprmlyontothe
pipettor.
The tip is damaged or worn
out due to extensive use.
Replace the tip with
a new one.
The pipettor
incorrectly
aspirates
the liquid
or the liquid
drops out
from the tip.
The pipettor tip is
incorrectly pressed onto
the pipettor shaft.
Press the pipet tip
rmlyontothe
pipettor.
The pipettor
incorrectly
aspirates
the liquid
or the liquid
drops out
from the tip.
The shaft surface is
damaged or contaminated
at the sealing site.
Clean the shaft or
replace it with a new
one.
The pipettor
incorrectly
aspirates
the liquid
or the liquid
drops out
from the tip.
The plunger or the O-ring is
damaged due to prolonged
aspiration of corrosive
liquids.
Disassemble the shaft
set; wash the shaft, the
plunger and the seal
(Section 10: Cleaning).
Replace the elements
with new ones if
necessary.
Apply a small amount
of lubricant onto
the plunger and
reassemble the set
in the correct order.
The inside of the pipettor is
contaminated.
The sealing elements are
notsufcientlylubricated.

14 – LABNET LABPETTE FX PIPETTORS
Problem Cause Solution
Uneven
work of the
pipetting set,
the pipetting
pushbutton
gets blocked.
The inside of the pipettor
is contaminated due to
aspiration of corrosive
substances.
Unscrew the shaft
set, wash the parts.
Replace the elements
with new ones if
necessary. Apply
a small amount
of lubricant on
the plunger and
reassemble the set
in the correct order.
The inside of the pipettor
is contaminated due to the
liquid entering the pipettor.
The sealing elements are
notsufcientlylubricated,
e.g. after repeated
autoclaving procedures.
Incorrect
aspiration.
Liquid with properties
other than water (density,
viscosity).
Calibrate the pipettor
using the liquid which
is to be pipetted.
Tipswithalterwith
increasedowresistance.
Calibrate the pipettor
using the tips which
are to be used for
pipetting.
If the problem persists after carrying out the above steps,
contact our regional representative.
Before returning the pipettor, please ensure the pipettor
is completely free of any chemical, radioactive, or
microbiological contamination which could pose a threat
during transport and repair.

Instruction Manual – 15
12. Spare Parts
Contact our Customer Service to inquire about availability
of spare parts. Pipettor model and name of the part
requiredshouldbespecied.Themostcommonpartsare
depicted below.
NOTE: The replacement of the plunger requires conducting
the calibration procedure according to Section 9.
AA
O
G
F1
I, J
C, F
D
O
D
G
C, F
F1
I, J
40-1,000 µL2-20 µL

16 – LABNET LABPETTE FX PIPETTORS
Item Description Model Cat. No. Qty/Pk
APipetting pushbutton 2 - 20
(red)
SP9175 1
40 - 200
(yellow)
SP9176 1
250 - 1,000
(green)
SP9177 1
C, F,
F1
Shaft Kit 2 - 20 SP9095 1
40, 50 SP9140 1
70 - 200 SP9031 1
250 - 500 SP9141 1
1,000 SP9032 1
DTip ejector 2 - 200 SP9178 1
250 - 1,000 SP9161 1
I, J Seal, O-ring and Bush
set
2 - 10 SP9054 1
20 SP9055 1
40, 50 SP9132 1
70 - 100 SP9070 1
120 - 200 SP9073 1
250 - 500 SP9133 1
1,000 SP9076 1
KCalibration key All SP9174 1

Instruction Manual – 17
13. Limited Warranty
Corning Incorporated (Corning) warrants that this product
will be free from defects in material and workmanship for
a period of three (3) years from date of purchase. CORNING
DISCLAIMS ALL OTHER WARRANTIES WHETHER EXPRESSED
OR IMPLIED, INCLUDING ANY IMPLIED WARRANTIES OF
MERCHANTABILITY OR OF FITNESS FOR A PARTICULAR
PURPOSE. Corning’s sole obligation shall be to repair or
replace, at its option, any product or part thereof that
proves defective in material or workmanship within the
warrantyperiod,providedthepurchasernotiesCorning
of any such defect. Corning is not liable for any incidental
or consequential damages, commercial loss or any other
damages from the use of this product.
This warranty is valid only if the product is used for its
intendedpurposeandwithintheguidelinesspecied
in the supplied instructions manual. This warranty does
not cover damage caused by accident, neglect, misuse,
improper service, natural forces or other causes not arising
from defects in the original material workmanship.
This warranty does not cover the O-ring, or shaft.
Claimsfortransitdamageshouldbeledwiththe
transportation carrier.
Intheeventthisproductfailswithinthespeciedperiodof
time because of a defect in material or workmanship, contact
Corning Customer Service at: CSEur[email protected],
visit www.labnetlink.com,orcontactyourlocalsupportofce.
Corning Customer Service will help arrange local service
where available or coordinate a return authorization
number and shipping instructions. Products received
without proper authorization will be returned. All items
returned for service should be sent using prepaid postage
in the original packaging or other suitable carton, padded
to avoid damage. Corning will not be responsible for
damage incurred by improper packaging. Corning may
elect for onsite service for larger equipment.
Some states do not allow limitation on the length of
implied warranties or exclusion or limitation of incidental
orconsequentialdamages.Thiswarrantygivesyouspecic
legal rights. You may have other rights which vary from state
to state.
No individual may accept for, or on behalf of Corning, any
other obligation of liability, or extend the period of this
warranty.

18 – LABNET LABPETTE FX PIPETTORS
For your reference, make a note of the model number, serial
number, date of purchase, and supplier here.
Model No. _______________________________________________
Serial No. ________________________________________________
Date Purchased ___________________________________________
Supplier _________________________________________________
Warranty/Disclaimer:Unlessotherwisespecied,all
products are for research use only. Not intended for use in
diagnostic or therapeutic procedures. Corning makes no
claims regarding the performance of these products for
clinical or diagnostic applications.
Product availability may vary per region.