Labnet Labpette FX User manual

Labnet Labpette FX
Single-channel, Fixed
Volume Pipettors
Instruction Manual
This manual is available in additional languages
at www.labnetlink.com.

Table of Contents
1. Product Description ...................................................................1
2. Packing..............................................................................................2
3. Pipettor Design.............................................................................2
4. Safety Recommendations ......................................................3
 5.Specications ................................................................................4
6. Pipettor Operation ......................................................................5
7. Operating Instructions .............................................................6
8. Aspiration and Dispensing Instructions...........................7
9. Checking Pipetting Accuracy Parameters
and Pipettor Recalibration.......................................................8
10. Pipettor Maintenance ........................................................... 12
11. Troubleshooting ....................................................................... 13
12. Spare Parts................................................................................... 15
13. Limited Warranty...................................................................... 17

Instruction Manual – 1
1. Product Description
LabnetLabpetteFXsingle-channel,xedvolumepipettoris
a volumetric instrument designed to measure and transfer
liquids precisely and safely. It can accommodate volumes
from 2 μL to 1,000 μL depending on the model.
Nominal Volume (µL) Cat. No. Color Code
2P3900-2 Red
5P3900-5 Red
10 P3900-10 Red
20 P3900-20 Yellow
40 P3900-40 Yellow
50 P3900-50 Yellow
70 P3900-70 Yellow
75 P3900-75 Yellow
100 P3900-100 Yellow
120 P3900-120 Yellow
150 P3900-150 Yellow
200 P3900-200 Yellow
250 P3900-250 Green
300 P3900-300 Green
400 P3900-400 Green
500 P3900-500 Green
1,000 P3900-1000 Green
Labnet pipettors operate using an air-cushion (i.e., the
aspirated liquid does not come into contact with the shaft
or plunger of the pipettor). The liquid is drawn into the
disposable tip attached to the pipettor.

2 – LABNET LABPETTE FX PIPETTORS
2. Packing
The pipettors are delivered with the following:
Description Qty/Pk
Instruction manual 1
Qualitycontrolcerticate 1
Calibration key 1
Shelf clip 1
Lubricant 1
3. Pipettor Design
Model Identication
The volume of the pipettor is shown on its pushbutton.
Pipetting pushbutton (A)
Ejector pushbutton (N)
Handle (O)
Tip ejector (D)
Shaft (C)

Instruction Manual – 3
4. Safety Recommendations
Long-term use of the pipettor relies on correct method of use.
Please read and follow the instructions for use carefully.
Symbols used:
Danger, risk of injury.
NOTE Risk of damage to the pipettor or errors in pipetting.
NOTE:
• The pipettor is designed for the transfer of liquids only
using the tip. Do not aspirate liquids without the tip
attached. The aspirated liquid should not enter the
pipettor, as it may cause damage.
• Single-use tips reduce the risk of contamination of
samples.
• Keep the pipettor clean, avoiding the use of abrasive or
corrosive cleaning agents (e.g., acetone).
• Keep the pipettor upright when there is liquid in the tip.
• Only using the pipettor in accordance with the
manufacturer’s instructions ensures the correct pipettor
parameters are maintained.
• After replacing the plunger or the shaft, the pipettor
should be calibrated.
• In the case of incorrect operation, the device should
be cleaned in accordance with the Instructions for Use
or transferred to a service point.
• Ambient operating temperature is +5°C to 45°C.
• Ambient storage conditions (in the original packaging
during transport and short storage) is -25°C to 55°C.
When working with the pipettor:
• Follow general work safety regulations regarding hazards
related to work in the laboratory.
• Take special care when pipetting aggressive substances.
• Use appropriate protective attire (e.g., clothing, goggles,
and gloves).
• Avoid pointing the pipettor at yourself or others during use.
• Only use parts and accessories recommended by the
manufacturer.

4 – LABNET LABPETTE FX PIPETTORS
5. Specications
The pipettor is a high quality instrument which offers
excellent accuracy and precision. The accuracy and precision
(repeatability) of the liquid volume depends on the quality
of pipet tips used. The values for accuracy and precision
shown in the table below were obtained using manufacturer
non-lterpipettips.Thosetipsarerecommendedforuseto
ensure compatibility, accuracy and precision when pipetting.
Nominal
Volume (µL) Cat. No.
Accuracy
(%)
Precision
(%)
Non-lter
Tips (µL)
2P3900-2 ±3.0 ≤1.5 200
5P3900-5 ±2.0 ≤1.0 200
10 P3900-10 ±1.2 ≤0.5 200
20 P3900-20 ±0.9 ≤0.4 200
40 P3900-40 ±0.9 ≤0.3 200
50 P3900-50 ±0.9 ≤0.3 200
70 P3900-70 ±0.9 ≤0.3 200
75 P3900-75 ±0.9 ≤0.3 200
100 P3900-100 ±0.9 ≤0.3 200
120 P3900-120 ±0.8 ≤0.3 200
150 P3900-150 ±0.7 ≤0.3 200
200 P3900-200 ±0.6 ≤0.2 200
250 P3900-250 ±0.6 ≤0.2 1,000
300 P3900-300 ±0.6 ≤0.2 1,000
400 P3900-400 ±0.6 ≤0.2 1,000
500 P3900-500 ±0.6 ≤0.2 1,000
1,000 P3900-1000 ±0.6 ≤0.2 1,000
The accuracy and precision were obtained gravimetrically
using manufacturer tips performing at least 10 measurements
of distilled water at a temperature of 20°C ± 1°C according to
EN ISO 8655 standards.
Theuseoftipsfromothermanufacturersorltertipsmay
result in incorrect liquid aspiration and require pipettor
recalibration.
The design of the pipettor enables the user to recalibrate it
according to the information presented in Section 9.

Instruction Manual – 5
6. Pipettor Operation
Attaching the Tips
• Attach the correct tip corresponding to the
model number displayed on the pipettor
ngerrest(Section5).
• Position the pipettor vertically when
attaching tips.
• Press the pipettor against the tip positioned
in the rack box. The suspension system
will ensure even and airtight sealing of the
pipet tips.
• Hold down the tip ejector button in order to
ensureatightertofthetip.
NOTE:
• Do not attach the tips with a rocking movement, as this
may damage the shaft or plunger.
• Never draw liquids directly into the pipettor without the
tip attached.
Ejecting the Tip
• Place your thumb against the ejector
pushbutton side and press it to the side.

6 – LABNET LABPETTE FX PIPETTORS
7. Operating Instructions
Observing the following recommendations will ensure
maximum possible accuracy and precision of liquid sampling.
• During operation, the volume setting should be locked,
with black adjustment knob in the lower position.
• Ensure smooth and slow operation of the pipettor.
• Immersion of the tip into the sample liquid should be
kept to a minimum depth, which should remain constant
during aspiration. The recommended immersion depths
are given in the table below:
Model Volume Range (µL) Immersion Depth (mm)
0.1-1 ≤1
1-100 2-3
101-1,000 2-4
• The pipettor should be held in a vertical position.
• The pipet tip should be changed whenever the volume
setting is altered, and when a different liquid is to be
aspirated.
• The pipet tip should be changed if a droplet remains on
the end of the tip from the previous pipetting operation.
• Each new pipet tip should be pre-rinsed with the liquid to
be pipetted.
• Liquid should never enter the pipettor shaft. To prevent this:
– Press and release the pushbutton slowly and smoothly.
– Never turn the pipettor upside down.
– Never lay the pipettor on its side when there is liquid in
the tip.
• Never force the volume setting beyond the recommended
limits.
• When pipetting liquids with a different temperature from
the ambient temperature, it is recommended to pre-rinse
the tip several times prior to use.
• Do not pipettor liquids with temperatures above 70°C.
NOTE: When pipetting acids or corrosive solutions which
emit vapors, it is recommended to disassemble the shaft
and rinse the plunger and O-ring with distilled water after
nishingthepipettingoperation.

Instruction Manual – 7
8. Aspiration and Dispensing Instructions
1. 2. 3. 5.4. 6.
Aspirating Liquid
1. Pressthepushbuttontotherststoppoint.
Holding the pipettor vertically, immerse the pipet tip
into the sample liquid to the recommended depth (for
recommended values see Section 7). If the pipet tip
is not immersed to the recommended depth or if the
pipetting pushbutton is rapidly released, air may enter
the pipet tip.
2. Release the pipetting pushbutton slowly and smoothly to
aspirate the sample. Wait one second and then withdraw
the pipet tip from the liquid.
CAUTION: Do not touch the used tip.
Dispensing Liquid
3. Place the end of the pipet tip against the inside wall of
the vessel at an angle of 10° to 40°. Press the pushbutton
smoothlytotherststop.Waitonesecond.
4. Press the pushbutton to the second stop to expel
any remaining liquid. While keeping the pushbutton
depressed, remove the pipettor from the vessel by
drawing the pipet tip against the inside surface of
the vessel.
5. Release the pushbutton to its starting position.
6. Eject the pipet tip by pressing the tip ejector pushbutton
to the side (left or right).
Remember to change the pipet tip whenever a different
type of liquid is to be sampled.

8 – LABNET LABPETTE FX PIPETTORS
Aspirating High-density Liquids
When pipetting liquids of higher viscosity or lower surface
tensionthanwater(e.g.,seraororganicsolvents),almof
liquid may be formed on the inside of the pipet tip which
mayproduceerroneousresults.Asthelmremainsrelatively
constant in successive pipetting operations with the same
tip, this error can be eliminated by pre-rinsing the tip and
allowingalmtoformbeforetransferringtherstsample.
This is achieved by aspirating a sample and dispensing it
backintothesamevessel.Allowingalmtoformpriorto
sampling ensures optimal accuracy and repeatability.
This pre-rinsing operation should be repeated when
the volume to be aspirated is changed or when a new pipet
tip is used.
NOTE: Normally the degree of error resulting from viscous
liquids is negligible if pipetting is performed slowly and
carefully, however can be minimized further by holding the
pipet tip in position for at least 2 seconds after aspiration
to allow the liquid time to react to the change in pressure
before it is dispensed.
If the above method does not result in accurate values,
recalibrate the pipettor in accordance with Section 9.
It is recommended to record recalibration and correction
values, in order to facilitate reverse calibration to a standard
liquid.
9. Checking Pipetting Accuracy Parameters
and Pipettor Recalibration
The pipettors have been factory-calibrated using gravimetric
methods with manufacturer pipet tips and distilled water,
in accordance with ISO 8655 guidelines
for the liquid volume drawn by the pipettor according to
the values given (Section 5). The pipettors are designed to
enable recalibration and adaptation to different pipetting
techniques and liquid properties (e.g., temperature, density,
and viscosity). Periodic checks of the operation of the
pipettor are recommended at least once per year. Frequency
of checks should be increased depending on workload,
sterilization or autoclave processes, and frequency of
replacement of component parts.
If during pipettor operation the accuracy error exceeds the
permissible value given in the table in Section 5, pipettor
recalibration should be carried out. Recalibration of the
pipettor involves adjusting the settings according to the
table on page 10.