Labnet ENDURO VE10 User manual

Copyright 2017 Labnet International, Version # 2
ENDURO™ VE10 Vertical Gel Electrophoresis Systems
User Manual
ENDURO VE10 System
E2010-PA
ENDURO VE10 Electroblotting System
E2010-PBA
Lit #M00608

Table of Contents
Page
Safety Precautions 1
Packing Lists 3
Care and Maintenance 5
Setting Up 6
Gel Casting 8
Gel Preparation 8
Gel Selection 9
Gel Pouring 10
Sample Preparation and Loading 11
Gel Running 12
Blotting Insert Setup 14
Blot Running 15
Appendix 20
Warranty 23
AccessoriesList 24

1
SAFETY PRECAUTION
WHEN USED CORRECTLY, THESE UNITS POSE NO HEALTH RISK.
HOWEVER, THESE UNITS CAN DELIVER DANGEROUS LEVELS OF ELECTRICITY AND ARE TO
BE OPERATED ONLY BY QUALIFIED PERSONNEL FOLLOWING THE GUIDELINES LAID OUT IN
THIS INSTRUCTION MANUAL.
ANYONE INTENDING TO USE THIS EQUIPMENT SHOULD READ THE COMPLETE MANUAL
THOROUGHLY.
The current to the unit, provided from external power supply, enters through the lid assembly,
providing a safety interlock to the user. When the lid is removed, the current to the unit is broken. DO
NOT attempt to use the unit without the safety lid correctly positioned. Always turn the power supply
off prior to removing the lid.
THE UNIT SHOULD NOT BE USED IF THERE IS ANY SIGN OF DAMAGE TO THE EXTERNAL
TANK OR LID.
ACRYLAMIDE IS A POWERFUL NEUROTOXIN IN SOLUTION FORM.
POLYMERIZED GELS CAN CONTAIN SOME UNPOLYMERIZED SOLUTION AND PROTECTIVE
GLOVES, SAFETY GLASSES AND CLOTHING MUST BE WORN.
THESE UNITS COMPLY WITH THE STATUTORY CE SAFETY DIRECTIVES:
73/23/EEC: LOW VOLTAGE DIRECTIVE: IEC 1010-1:1990 plus AMENDMENT 1:1992
EN 61010-1:1993/BS EN 61010-1:1993

2
Important Notice
Care and Maintenance:
These products and associated accessories should never come into
contact with the following cleaning agents, as these will cause
irreversible and accumulative damage:
Acetone
Chloroform
Methanol
Carbon tetrachloride
Phenol
Alkalis
Ethanol
Isopropyl alcohol
Water at temperatures above 60°C will cause damage to the acrylic
tanks, trays and other parts.
The tanks should be thoroughly rinsed with warm or distilled water but vigorous
cleaning is not necessary or advised. Air drying is recommended before use.
Cleaning Products and Accessories
Products are best cleaned using warm water and a mild detergent.
The units should only be cleaned with the following:
Warm water with a low concentration of soap or other compatible mild detergent.
Compatible detergents include dishwashing liquid, Hexane and Aliphatic
hydrocarbons.
The units should not be left in detergents for more than 30 minutes

3
PACKING LISTS:
ENDURO VE10 System
Units include tank, lid, internal module and electrodes and include the following
accessories:
Glas
s Plates
Combs
Casting
Base
Cooling
Pack
Cables
E2010
-
PA
VE10 Vertical
Gel System
(E2110-NG-2) Glass
Plates, Notched, Pk/2
(E2110-PG-1-BS) Glass
Plates, Plain with bonded
1mm spacers, Pk/2
(E2110-DP) Dummy Plate
(E2110-12-1)
qty of 2, 1mm
thick, 12 sample
combs
E2110-PC E2110-CP E1107-EP
ENDURO VE10 Electroblotting System
Units include tank, lid, internal module, electrodes, Blotting module and include the
following accessories:
Glass Plates
Combs
Casting
Base
Cooling
Pack
Cables
ENDURO VE10
Electroblotting
System
(E2110-NG-2) Glass
Plates, Notched, Pk/2
(E2110-PG-1-BS) Glass
Plates, Plain with
bonded 1mm spacers,
Pk/2
(E2110-DP) Dummy
Plate
(E2110-12-1)
qty of 2, 1mm
thick, 12 sample
combs
E2110-PC E2110-CP E1107-EP
Electroblotting
module
(E2110-B-MC) 4 Blotting Cassettes, (E2110-B-FP) Pack of 8 Fiber pads

4
The packing lists should be referred to as soon as the units are received to
ensure that all components have been included. The unit should be checked
for damage when received. If damaged, contact carrier and save the box for
inspection. Please contact Labnet International if there are any problems or
missing items.
Usage Guidance and restrictions:
• Maximum altitude 2,000m.
• Temperature range between 4°C and 60°C.
• Maximum relative humidity 80% for temperatures up to 31°C decreasing linearly to
50% relative humidity at 40°C.
• Not for outdoor use.
This apparatus is rated POLLUTION DEGREE 2 in accordance with IEC 664.
POLLUTION DEGREE 2, states that: “Normally only non-conductive pollution
occurs. Occasionally, however, a temporary conductivity caused by condensation
must be expected.”
Care and Maintenance:
Cleaning ENDURO VE10 System
Units are best cleaned using warm water and a mild detergent. Water at
temperatures above 60°C can cause damage to the unit and components. The
tank should be thoroughly rinsed with warm water and distilled water to prevent build
up of salts but care should be taken not to damage the enclosed electrode.
Vigorous cleaning is not necessary or advised. Air drying is recommended before
use.
The units should never come into contact with the following cleaning agents,
these will cause irreversible and cumulative damage:-

5
Acetone, Phenol, Chloroform, Carbon tetrachloride, Methanol, Ethanol, Isopropyl
alcohol, Alkalis.
RNase Decontamination
This can be performed using the following protocol:-
Clean the units with a mild detergent as described above.
Wash with 3% hydrogen peroxide (H2O2) for 10 minutes.
Rinse with 0.1% DEPC- (diethyl pyrocarbonate) treated distilled water,
Caution: DEPC is a suspected carcinogen. Always wear gloves and safety
glasses.
RNaseZAP™ (Ambion) can also be used. Please consult the instructions for use
with acrylic gel tanks.
Questions and Service:
Should you have a question about the operation of the Spectrafuge Mini Centrifuge or
if service is required, contact Corning at: 800-492-1110. Do not send in a unit for
service without first calling to obtain a repair authorization number. Should the unit
require return to Corning for service, it should be properly packed to avoid damage.
Any damage resulting from improper packaging shall be the responsibility of the
user.

6
Setting up the ENDURO VE10 Gel Tanks:
Instructions for installing Electrical Leads.
1. Note the position of the lid on the unit. This shows the correct polarity and the
correct orientation of the cables, black is negative and red positive.
2. Remove the lid from the unit. Note if the lid is not removed, fitting the cables may
result in un-tightening of the gold plug and damage to the electrode.
3. Screw the cables into the tapped holes in the lid as fully as possible so that there
is no gap between the lid and the leading edge of the cable fitting.
4. Refit the lid.
The unit is now ready for use.
Vertical Gel Casting Using the ENDURO VE10 Gel Casting System:-
1. Clean a set of glass plates for each gel first with distilled water and then with
70% ethanol. One set of glass plates constitutes one notched glass plate and
one plain glass plate with bonded spacers. When using a triple glass plate
sandwich, two notched glass plates, one set of free spacers and a set of plain
glass plates with bonded spacer. The plain glass plate is positioned outermost,
then a notched glass plate, free spacers and second notched glass plate.
Alternatively, accessory notched glass plates with bonded spacers are available.
All glass plates, modules and casting base accessories must be completely
dry for setup. Wet components are more likely to misalign and cause leaks.
2. Assemble the glass plates so that the bottom of the glass plates and the spacers
are perfectly aligned. For triple plate sandwiches, the free spacers need to be
perfectly aligned which is best performed using a small spacer or comb to push
the spacers apart. Notched glass plates with bonded spacers do not need

7
manual alignment. NOTE: The spacers bonded to the glass plates are
slightly longer than the plates and marked with an arrow to indicate the top.
3. The ENDURO VE10 Gel insert contains pressure bars which impart even
pressure onto the edge of the glass plate. Ensure that the pressure bars are
adequately open for the thickness of spacer used. The bar can be opened by
sliding open clamps. When using a triple glass plate sandwich, the pressure bars
will need to be in the completely open position.
4. Position the ENDURO VE10 Gel module on a flat surface. Do not insert the
ENDURO VE10 Gel module into the casting base at this point.
5. Place the glass plate/spacer assembly into the Vertical Gel Insert between the
pressure bar and the blue gasket. Check that the bottoms of the glass plates are
touching the bench and fully tighten the sliding clamps. When only one gel is
being run, the dummy plate must be used in the second position and fully
tightened. NOTE: Be sure that the glass plates and the bottom of the
spacers are evenly aligned.

8
6. Open the cam handles on the casting stand and position the cams so they face
downwards. Position the ENDURO VE10 Gel insert on the gasket of the casting
stand so the key holes are facing the cams. The top of the ENDURO VE10 Gel
insert will need to be pushed down very slightly to insert the cams.
7. With the cam handles facing directly downwards, rotate the cams in the opposite
direction 1800 or until the insert has tightened down onto the gasket. Do not
overturn as this will cause the glass plates to push upwards and the
assembly will be more likely to leak.
NOTE: Always reverse the gasket after casting to avoid indentations. Do not
leave glass plates tightened into the casting stand for long periods of time as
this can cause damage the silicone mat. The unit is now ready for gel
preparation.