Leica HistoCore Arcadia C User manual

Instructions for Use
HistoCore Arcadia C
Cold Plate
HistoCore Arcadia C V 1.7, English 12/2017
Order No.: 14 0393 80101 Rev. J
Always keep this manual with the instrument.
Read carefully before working with the instrument.

1
HistoCore Arcadia C – Cold Plate
The information, numerical data, notes and value
judgments contained in this manual represent
the current state of scientific knowledge and
state-of-the-art technology as we understand it
following thorough investigation in this field.
We are under no obligation to update the present
manual periodically and on an ongoing basis
according to the latest technical developments,
nor to provide our customers with additional
copies, updates etc. of this manual.
To the extent permitted in accordance with
the national legal system as applicable in each
individual case, we shall not be held liable for
erroneous statements, drawings, technical
illustrations etc. contained in this manual. In
particular, no liability whatsoever is accepted
for any financial loss or consequential damage
caused by or related to compliance with state-
ments or other information in this manual.
Statements, drawings, illustrations and other
information regarding the contents or technical
details of the present Instructions for Use are
not to be considered warranted characteristics
of our products.
These are determined only by the contract
provisions agreed between ourselves and our
customers.
Leica Biosystems reserves the right to change
technical specifications as well as manufactur-
ing processes without prior notice. Only in this
way is it possible to continuously improve the
technology and manufacturing techniques used
in our products.
This document is protected under copyright
laws. All copyrights to this documentation are
held by Leica Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or
of any parts thereof) by means of print, photo-
copy, microfiche, web cam or other methods—
including any electronic systems and media—
requires express prior permission in writing by
Leica Biosystems Nussloch GmbH.
For the instrument serial number and year of
manufacture, please refer to the name plate at
the back of the instrument.
© Leica Biosystems Nussloch GmbH
-NOTE
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Phone: [phone]
Fax: [phone]
Internet: http://www.LeicaBiosystems.com
Assembly contracted to Leica Microsystems Ltd. Shanghai

2Instructions for Use V 1.7 Rev. J – 12/2017
Table of contents
1. Important Information ................................................................................................................. 3
1.1 Naming conventions .................................................................................................................... 3
1.2 Symbols in the text and their meanings.................................................................................... 3
1.3 Instrument type............................................................................................................................. 6
1.4 Intended use of instrument......................................................................................................... 6
1.5 Qualification of personnel........................................................................................................... 6
2. Safety ............................................................................................................................................. 7
2.1 Safety notes................................................................................................................................... 7
2.2 Warnings........................................................................................................................................ 8
3. Instrument Components and Specifications......................................................................... 10
3.1 Overview — instrument components ..................................................................................... 10
3.2 Main features of the instrument............................................................................................... 10
3.3 Technical Data............................................................................................................................ 11
4. Setting up the instrument ........................................................................................................ 13
4.1 Site requirement ......................................................................................................................... 13
4.2 Standard delivery—packing list............................................................................................... 13
4.3 Unpacking and installation........................................................................................................ 15
4.4 Moving the instrument............................................................................................................... 16
4.5 Power supply............................................................................................................................... 17
5. Operation..................................................................................................................................... 18
5.1 Switching the instrument on..................................................................................................... 18
5.2 Replacing the secondary fuse.................................................................................................. 18
6. Maintenance and Cleaning...................................................................................................... 19
6.1 Cleaning the instrument............................................................................................................. 19
6.2 Maintenance instructions......................................................................................................... 19
7. Troubleshooting ......................................................................................................................... 20
8. Warranty and Service................................................................................................................ 21
9. Decontamination Confirmation............................................................................................... 22

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HistoCore Arcadia C – Cold Plate
1.2 Symbols in the text and their meanings
Warnings
appear in a gray box and are marked by a warning triangle .
Notes,
i.e. important user information appear in a gray box and are marked by an
information symbol .
(5)
(Figure 5)
Numbers in parentheses refer to item numbers in illustrations or to the illustrations
themselves.
Caution, consult the instructions for use for cautionary information.
Power on.
Power off.
Observe the Instructions for Use
Manufacturer
Date of manufacture
Symbol for alternating current
PE terminal
Order No.
1.1 Naming conventions
The full name of the device is HistoCore Arcadia C Cold Plate. The device is called
HistoCore Arcadia C to ensure that the Instructions for Use are well legible.
1. Important Information

4Instructions for Use V 1.7 Rev. J – 12/2017
1. Important Information
Serial number
Environmental protection symbol of the China RoHS directive. The number in
the symbol indicates the "Environment-friendly Use Period" of the product. The
symbol is used if a substance restricted in China is used in excess of the max-
imum permitted limit.
Symbol for labeling electrical and electronic equipment in accordance with Sec-
tion 7 of the German Electrical and Electronic Equipment Act (ElektroG). ElektroG is
the law regarding the sale, return and environmentally sound disposal of electrical
and electronic equipment.
This product fulfills the requirements of the EU directives.
This product fulfills the requirements of the CAN/CSA-C22.2 No. 61010.
The package contents are fragile and must be handled with care.
The package must be kept in a dry environment.
Indicates the correct upright position of the package.
It allows maximum 2 stacks layers.
1.2 Symbols in the text and their meanings

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HistoCore Arcadia C – Cold Plate
1. Important Information
1.2 Symbols in the text and their meanings (Continued)
Indicates the temperature range permitted for storing and transporting the
package.
Minimum -29 °C
Maximum +50 °C
Indicates the temperature range permitted for storing the package.
Minimum +5 °C
Maximum +50 °C
Indicates the humidity range permitted for storing and transporting the
package.
Minimum 10 % r.H.
Maximum 85 % r.H
Tip-n-Tell indicator to monitor whether the shipment has been transported and
stored in upright position according to your requirements. With a pitch of 60° or
more, the blue quartz sand flows into the arrow-shaped indicator window and
sticks there permanently. Improper handling of the shipment is immediately
detectable and can be proven definitively.
In the Shockwatch system, a shock dot shows shocks or impacts that are above
a specified intensity through red coloration. Exceeding a defined acceleration (g
value) causes the indicator tube to change color.
Indicates the item can be recycled where correct facilities exist.
The Regulatory Compliance Mark (RCM) indicates a device’s compliance with
applicable ACMA technical standards of New Zealand and Australia - that is,
for telecommunications, radio communications, EMC and EME.

6Instructions for Use V 1.7 Rev. J – 12/2017
1. Important Information
1.4 Intended use of instrument
The HistoCore Arcadia C is a cold plate for chilling and blocking out histological tissue samples
in paraffin blocks.
Any other use of the instrument will be considered as improper use!
1.5 Qualification of personnel
• The HistoCore Arcadia C may be operated by trained laboratory personnel only.
• All laboratory personnel designated to operate this instrument must read these Instructions
for Use carefully and must be familiar with all technical features of the instrument before
attempting to operate it.
Fig.1
Fig. 1 is provided as an example only
and shows a valid nameplate for this
instrument with the necessary informa-
tion about instrument type and power
requirement. The precise data for the
various versions is specified in Chap-
ter 3.3, "Technical data".
1.3 Instrument type
All information provided in these Instruc-
tions for Use applies only to the instrument
type indicated on the cover page.
A nameplate is attached to the back of the
instrument and a serial number label is on
the side of the instrument.

7
HistoCore Arcadia C – Cold Plate
These Instructions for Use include important instructions and information related to the oper-
ating safety and maintenance of the instrument.
These Instructions for Use are an important part of the product, and must be read carefully
prior to startup and use and must always be kept near the instrument.
This instrument has been built and tested in accordance with the safety requirements for elec-
trical equipment for measurement, control, and laboratory use.
To maintain this condition and ensure safe operation, the user must observe all notes and
warnings contained in these Instructions for Use.
2. Safety
2.1 Safety notes
The safety and caution notes in this chapter must be observed at all times.
Be sure to read these notes even if you are already familiar with the operation and use
of other Leica Biosystems products.
These Instructions for Use must be appropriately supplemented as required by the exist-
ing regulations on accident prevention and environmental safety in the operator's coun-
try.
The protective devices on the instrument and its accessories must not be removed or
modified. Only service personnel qualified by Leica Biosystems may repair the instru-
ment and access the instrument's internal components.
Use only the provided power cable - this must not be replaced with a different power
cable. If the power plug does not fit in your socket, contact our service.
Residual risks
The instrument has been designed and constructed with the latest state-of-the-art tech-
nology and according to recognized standards and regulations with regard to safety
technology. Operating or handling the instrument incorrectly can place the user or other
personnel at risk of injury or can cause damage to the instrument or other property. The
instrument may be used only as intended and only if all of its safety features are in prop-
er working condition. Malfunctions that impede safety must be remedied immediately.
For current information about applicable guidelines, please refer to the CE declaration
of conformity and on our Internet site at:
http://www.LeicaBiosystems.com

8Instructions for Use V 1.7 Rev. J – 12/2017
2. Safety
2.2 Warnings
The safety devices installed in this instrument by the manufacturer only constitute the basis
for accident prevention. Operating the instrument safely is, above all, the responsibility of the
owner, as well as the designated personnel who operate, service or repair the instrument.
To ensure trouble-free operation of the instrument, make sure to comply with the following
instructions and warnings.
Warnings – Safety notes on the instrument itself
• This device may only be used by trained laboratory technicians. It must only be
operated for the purpose of its designated use and according to the instructions
contained in these Instructions for Use.
• Safety notes on the instrument itself, which are marked with a warning tri-
angle, indicate that the correct operating instructions (as defined in these
Instructions for Use) must be followed when operating or replacing the item
marked. Nonobservance can cause accidents, injuries and/or damage to the
instrument/accessories.
To prevent damage to the instrument or the specimen, only accessories authorized by
Leica Biosystems may be used.
Safety instructions - Transport and installation
• After unpacking the instrument it may only be transported in an upright position.
• Before connecting the device to a power source, ensure that the voltage indicat-
ed on the type plate matches the voltage available at the place of installation.
• The unit must be connected only with the supplied power cable and only to a
grounded power receptacle. Do not use an extension cord.
• The power socket to which the instrument is connected has to be near the
instrument and easily accessible.

9
HistoCore Arcadia C – Cold Plate
2. Safety
• The minimum voltage (see Technical data) must be maintained while starting
the refrigeration unit.
The compressor needs a start-up current of approx. 25 A. A stable power supply
in accordance with the instrument's specifications is essential to its proper function-
ing. Please ensure that your electrical installation fulfills these preconditions prior to
installing the unit. Nonobservance causes damage to the instrument.
• Switch off the instrument each time before servicing, repairing or cleaning,
and pull out the power plug.
• Failure to observe the instructions specified by the manufacturer may result in
damage to the protection provided by the instrument.

10 Instructions for Use V 1.7 Rev. J – 12/2017
3. Instrument Components and Specifications
3.1 Overview — instrument components
Fig.2
Instrument front view
3.2 Main features of the instrument
• The instrument is distinguished by a simple, modular design and a powerful refrigeration unit
with precisely controlled cooling performance.
• The environment adaptive control module ensures the working temperature always stabilized
at -6 °C.
• High cooling performance ensures that the instrument's working temperature is reached
quickly.
• Optimized temperature distribution in the cold plate prevents dripping condensation.
• The generously-dimensioned cooling surface has room for around 60 blocks.
• Designed to be used with the HistoCore Arcadia H Paraffin Embedding Station.
1
3
2
1. Power switch 4. Heat sink
2. Refrigeration system (inside) 5. Port for power inlet
3.Cooling surface 6. AC fuses
4
6
5

11
HistoCore Arcadia C – Cold Plate
3. Instrument Components and Specifications
3.3 Technical Data
General data
Voltage of power supply 100 VAC, 110-120 VAC, 220-240 VAC, 50/60Hz
Fuse Time-lag fuses 5 x 20 mm
• 220-240 VAC: 2xT5A, 250V
• 100-120 VAC: 2xT10A, 250V
Nominal current 5 A max.
Maximum start-up current (5 s) 25 A
Environmental operating temperature range +20 °C to +30 °C
Operating temperatures -6 °C
Environmental relative humidity 20 to 80 % - non-condensing
Environmental operating altitude Up to 2000 m
Permissible temperature range
during storage
+5 °C to +50 °C
Permissible temperature range
during transport
-29 °C to +50 °C
Permissible humidity range
during storage and transport
10 to 85 % - non-condensing
Electromagnetic environment Basic electromagnetic environment
IEC 61010 classification Protection class 1
Pollution degree 2
IP protection class (IEC 60529) IP20
Refrigeration unit
Refrigeration capacity* 158 W( at 50Hz) ;185 W( at 60Hz)
Safety factor 3
Refrigerant R 134a
Compressor oil 150 +10/-5 ml Ester RL7H, ISO 7
* according to ASHRAE, condensing temperature: 54.4 °C, evaporating temperature: -23.3 °C
Coolant 115 g ± 2 g

12 Instructions for Use V 1.7 Rev. J – 12/2017
3. Instrument Components and Specifications
3.3 Technical Data (Continued)
Dimensions and weights
for instrument Order No. 14 0393 53599, 14 0393 54102, 14 0393 54092,14 0393 54091
Width: 400 mm
Depth: 605 mm
Height: 385 mm
Weight: 30 kg
Dimensions and weights
for instrument Order No. 14 0393 57262, 14 0393 57261, 14 0393 57260, 14 0393 57263
Width: 400 mm
Depth: 636 mm
Height: 384 mm
Weight: 32 kg

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HistoCore Arcadia C – Cold Plate
4. Setting up the instrument
• Stable, vibration-free laboratory table with horizontal, flat table top, as far as possible vi-
bration-free ground.
• No direct sunlight or strong temperature fluctuations. Room temperature consistently be-
tween +20 °C and +30 °C.
• Relative air humidity maximum 80 %, non-condensing.
• The instrument should be set up in such a way that the air circulation is not impaired.
• The instrument must be installed in a place that ensures an easy disconnection from the
power supply. The power cable must be in a place that can be easily reached.
4.1 Site requirement
At a room temperature of > +30 °C, the working temperature of the cold plate of -6°C
may not be reached at all points.
To ensure proper function and an easy disconnection of the power cable from the in-
strument, there must be gap of at least 15 cm behind the instrument. Failure to observe
this distance may result in serious damage to the refrigeration unit of the device. The
instrument should not be operated in hazardous locations.
4.2 Standard delivery—packing list
Qty Designation Order No.
1 Basic unit HistoCore Arcadia C,
220-240 VAC 14 0393 53599
220-240 VAC, China 14 0393 54102
110-120 VAC 14 0393 54092
100 VAC 14 0393 54091
220-240 VAC 14 0393 57262
220-240 VAC, China 14 0393 57263
110-120 VAC 14 0393 57261
100 VAC 14 0393 57260
4 Sets of spare fuses: 220-240 VAC, 5A 250 V 14 6000 05015
100-120 VAC, 10A 250V 14 6000 05078
1Instructions for Use (printed German/English with language CD 14 0393 80200) 14 0393 80001

14 Instructions for Use V 1.7 Rev. J – 12/2017
4. Setting up the instrument
Please compare the delivered components against the packing list and your order. Should
there be any discrepancy, please contact the Leica Biosystems distributor handling your
order.
The country specific power cord needs to be ordered separately. Please find a list of all power cords
available for your device on our website www.LeicaBiosystems.com within the product section.

15
HistoCore Arcadia C – Cold Plate
4.3 Unpacking and installation
4. Setting up the instrument
1. Remove the packing strap (1) and the adhesive
tape (2).
2. Open the package. Lift up and remove the
carton wall (3).
3. Remove the foam pads (4) one by one.
These unpacking instructions only
apply if the box is placed with the
symbols facing upwards.
The HistoCore Arcadia C always has
to be transported upright and horizon-
tally. It must not be inverted under any
circumstances, even for short periods,
or stored on one of its sides.
It is mandatory to observe a wait-
ing time of 4 hours between the
last transport and the first time the
instrument is switched on. The oil
present in the compressor needs
this time to flow back to its original
location.
Fig.3
4
Fig.4
When the instrument is delivered, check the tilt indicators on the packaging.
If the arrowhead is blue, the shipment was transported laying flat, was tilted at
too great an angle or fell over during transport.
Note this on the shipping documents and check the shipment for possible damage.
1
2
3
4

16 Instructions for Use V 1.7 Rev. J – 12/2017
4. Setting up the instrument
4.3 Unpacking and installation (Continued)
Fig.5
5
The packaging must be retained for
the duration of the warranty period.
To return the instrument, follow the
instructions above in reverse order.
4. Ensure that when removing the instrument (5)
from the pallet this is carried out by two people
lifting four lower corners of the housing base
(Fig. 5).
5. Place the instrument on a stable laboratory
table.
6. Remove the accessories from the accessory
box (6) on the base of the pallet.
Fig.6
6
4.4 Moving the instrument
Do not move the instrument during operation.
Before moving the instrument, make sure that there is no specimen blocks on the cold
plate, the instrument is at an ambient temperature, and the power cord is disconnected
from the power supply.
Do not touch the metal parts of the compressor air outlet (1) on the rear panel.
It is mandatory to observe a waiting time of 4 hours before the instrument is switched
on.
Hold the instrument at the front and rear part of
the lower housing base and move.
1
Fig.7

17
HistoCore Arcadia C – Cold Plate
4. Setting up the instrument
Fig.8
• Connect the power cord plug (7) to the
connecting port (6).
• Plug the power cord into the wall outlet.
4.5 Power supply
The HistoCore Arcadia C refrigeration unit requires a specific voltage and frequency (refer to
"Technical data" chapter), and is therefore always delivered with a power cord that fits the
instrument.
Please observe the following notes to prevent damage to the instrument.
Before connecting the instrument to the power supply, it is mandatory to check whether
the voltage specified on the identification label (rear side) matches the actual voltage
values at the installation location.
If this is not the case, the connection must not be made!
The unit must be connected only with the supplied power cord and only to a grounded
power receptacle.
Do not use an extension cord!
6
7

18 Instructions for Use V 1.7 Rev. J – 12/2017
5. Operation
5.1 Switching the instrument on
After installation as described in Chapter 4, the HistoCore Arcadia C is ready for
operation. Switch on the device with the Power switch at the front left of the
instrument ("I" = ON). A lamp lights up in the switch to indicate that the unit is op-
erational and the refrigeration unit will start working.
Depending on the room temperature, the time to reach the target temperature of
the cooling surface (-6 °C) will be around 25 minutes.
Fig.9
The cooling surface may not be loaded with molds until the cooling time
has elapsed. Otherwise, the working temperature of -6°C may not be
reached.
A miniature fuse to protect the electronic components is
located on the rear of the instrument.
Fuse rating: 220-240 VAC, 5A 250 V
100-120 VAC, 10A 250 V
To replace the fuse, please proceed as follows:
1. Use a screwdriver to open the fuse holder (9) and
remove the fuses (10).
2. Replace them with two new fuses of the same type.
3. Use the screwdriver to press the fuse holder back to
its original location.
4. Reconnect the instrument to an AC power outlet and
switch it on.
9
5.2 Replacing the secondary fuse
10
Before replacing the fuse, always switch the
instrument off and pull the power plug from the
wall socket.
Only miniature fuses of the type specified in the
chapter "Technical data" can be used.
Fig.10
The compressor will start to work in five minutes after the power is on.