Leica HistoCore Arcadia H User manual

Instructions for Use
HistoCore Arcadia H
Paraffin Embedding Station
HistoCore Arcadia H V 1.7, English 12/2017
Order No.: 14 0393 81101 Rev. J
Always keep this manual with the instrument.
Read carefully before working with the instrument.

1
HistoCore Arcadia H – Paraffin Embedding Station
The information, numerical data, notes and val-
ue judgments contained in this manual represent
the current state of scientific knowledge and
state-of-the-art technology as we understand it
following thorough investigation in this field.
We are under no obligation to update the pres-
ent manual periodically and on an ongoing basis
according to the latest technical developments,
nor to provide our customers with additional
copies, updates etc. of this manual.
To the extent permitted in accordance with
the national legal system as applicable in each
individual case, we shall not be held liable for
erroneous statements, drawings, technical illus-
trations etc. contained in this manual. In partic-
ular, no liability whatsoever is accepted for any
financial loss or consequential damage caused
by or related to compliance with statements or
other information in this manual.
Statements, drawings, illustrations and other
information regarding the contents or technical
details of the present Instructions for Use are
not to be considered warranted characteristics
of our products.
These are determined only by the contract
provisions agreed between ourselves and our
customers.
Leica Biosystems reserves the right to change
technical specifications as well as manufactur-
ing processes without prior notice. Only in this
way is it possible to continuously improve the
technology and manufacturing techniques used
in our products.
This document is protected under copyright
laws. All copyrights to this documentation are
held by Leica Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or
of any parts thereof) by means of print, photo-
copy, microfiche, web cam or other methods—
including any electronic systems and media—
requires express prior permission in writing by
Leica Biosystems Nussloch GmbH.
For the instrument serial number and year of
manufacture, please refer to the name plate at
the back of the instrument.
© Leica Biosystems Nussloch GmbH
-NOTE
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Phone: [phone]
Fax: [phone]
Internet: http://www.LeicaBiosystems.com
Assembly contracted to Leica Microsystems Ltd. Shanghai

2Instructions for Use V 1.7 Rev. J – 12/2017
Table of contents
1. Important Information ................................................................................................................. 4
1.1 Naming conventions .................................................................................................................... 4
1.2 Symbols in the text and their meanings.................................................................................... 4
1.3 Instrument type............................................................................................................................. 8
1.4 Intended use of instrument......................................................................................................... 8
1.5 Qualification of personnel........................................................................................................... 8
2. Safety ............................................................................................................................................. 9
2.1 Safety notes................................................................................................................................... 9
2.2 Warnings...................................................................................................................................... 10
2.3 Integrated safety devices.......................................................................................................... 11
3. Instrument Components and Specifications......................................................................... 12
3.1 Overview — Instrument components..................................................................................... 12
3.2 Main features of the instrument............................................................................................... 13
3.3 Technical Data............................................................................................................................ 14
4. Setting up the instrument ........................................................................................................ 16
4.1 Site requirement ......................................................................................................................... 16
4.2 Standard delivery—packing list............................................................................................... 17
4.3 Unpacking and installation........................................................................................................ 19
4.4 Necessary assembly work........................................................................................................ 21
4.5 Electrical connection................................................................................................................. 23
4.6 Moving the instrument............................................................................................................... 23
5. Operation..................................................................................................................................... 24
5.1 Instrument parts/functions........................................................................................................ 24
5.2 Switching the instrument on..................................................................................................... 28
5.3 Control panel functions.............................................................................................................. 29
5.4 Operation modes......................................................................................................................... 33
5.5 Instrument heater ...................................................................................................................... 34
6. Maintenance and Cleaning...................................................................................................... 35
6.1 Cleaning the instrument............................................................................................................. 35
6.2 Maintenance instructions......................................................................................................... 36
7. Troubleshooting ......................................................................................................................... 37
7.1 Error message............................................................................................................................. 37
7.2 Possible faults............................................................................................................................. 42
7.3 Changing a fuse .......................................................................................................................... 44
8. Optional Accessories ................................................................................................................ 45
8.1 Magnifier...................................................................................................................................... 45

3
HistoCore Arcadia H – Paraffin Embedding Station
8.2 Foot switch................................................................................................................................... 46
8.3 Pre-filter cup................................................................................................................................ 47
8.4 Basket handle.............................................................................................................................. 47
8.5 Ordering information.................................................................................................................. 47
9. Warranty and Service ............................................................................................................... 48
10. Decontamination Confirmation .............................................................................................. 49
Table of contents

4Instructions for Use V 1.7 Rev. J – 12/2017
1.2 Symbols in the text and their meanings
Warnings
appear in a gray box and are marked by a warning triangle .
Notes,
i.e. important user information appear in a gray box and are marked by an
information symbol .
(5)
(Figure 5)
Numbers in parentheses refer to item numbers in illustrations or to the
illustrations themselves.
Function keys, that have to be pressed on the display screen, are displayed in
bold type and capital letters.
This warning symbol indicates the surfaces on the instrument that are hot
during operation.
Avoid direct contact to prevent risk of burning.
Solvents and reagents that are inflammable are marked with this symbol.
Caution, consult the instructions for use for cautionary information.
Caution, consult the instructions for use for cautionary information.
Power on.
Power off.
1.1 Naming conventions
The full name of the device is HistoCore Arcadia H Paraffin Embedding Station. The device is
called HistoCore Arcadia H to ensure that the Instructions for Use are well legible.
1. Important Information

5
HistoCore Arcadia H – Paraffin Embedding Station
1. Important Information
1.2 Symbols in the text and their meanings (Continued)
Observe the Instructions for Use.
Manufacturer
Date of manufacture
Symbol for alternating current
PE terminal
Order No.
Serial number
Environmental protection symbol of the China RoHS directive. The number in
the symbol indicates the "Environment-friendly Use Period" of the product. The
symbol is used if a substance restricted in China is used in excess of the max-
imum permitted limit.
Symbol for labeling electrical and electronic equipment in accordance with Section
7 of the German Electrical and Electronic Equipment Act (ElektroG). ElektroG is the
law regarding the sale, return and environmentally sound disposal of electrical and
electronic equipment.
This product fulfills the requirements of the EU directives.
This product fulfills the requirements of the CAN/CSA-C22.2 No. 61010.
The package contents are fragile and must be handled with care.

6Instructions for Use V 1.7 Rev. J – 12/2017
1. Important Information
The package must be kept in a dry environment.
Indicates the correct upright position of the package.
It allows maximum 2 stacks layers.
Indicates the temperature range permitted for transporting the package.
Minimum -29 °C
Maximum +50 °C
Indicates the temperature range permitted for storing the package.
Minimum +5 °C
Maximum +50 °C
Indicates the humidity range permitted for storing and transporting the
package.
Minimum 10 % r.H.
Maximum 85 % r.H
1.2 Symbols in the text and their meanings (Continued)

7
HistoCore Arcadia H – Paraffin Embedding Station
1. Important Information
Tip-n-Tell indicator to monitor whether the shipment has been transported and
stored in upright position according to your requirements. With a pitch of 60° or
more, the blue quartz sand flows into the arrow-shaped indicator window and
sticks there permanently. Improper handling of the shipment is immediately
detectable and can be proven definitively.
In the Shockwatch system, a shock dot shows shocks or impacts that are above
a specified intensity through red coloration. Exceeding a defined acceleration (g
value) causes the indicator tube to change color.
Indicates the item can be recycled where correct facilities exist.
The Regulatory Compliance Mark (RCM) indicates a device’s compliance with
applicable ACMA technical standards of New Zealand and Australia - that is,
for telecommunications, radio communications, EMC and EME.
1.2 Symbols in the text and their meanings (Continued)

8Instructions for Use V 1.7 Rev. J – 12/2017
1.4 Intended use of instrument
The HistoCore Arcadia H is a modern paraffin embedding station with a microprocessor con-
trol system.
The HistoCore Arcadia H is designed for embedding histological tissue specimens in molten
paraffin for use in pathology laboratories
It is only for the following tasks:
• Melt solid paraffin for sample embedding and maintain the molten paraffin at the required
temperature.
• Dispense paraffin into embedding molds where the specimens are placed.
• Heat and maintain the temperatures of embedding cassettes with specimens and molds as
well as the required forceps.
Any other use of the instrument will be considered as improper use!
1.5 Qualification of personnel
• The HistoCore Arcadia H may be operated by trained laboratory personnel only.
• All laboratory personnel designated to operate this instrument must read these Instructions
for Use carefully and must be familiar with all technical features of the instrument before
attempting to operate it.
Fig.1
Fig. 1 is provided as an example only and shows a valid nameplate for this instrument
with the necessary information about instrument type and power requirement. The pre-
cise data for the various versions is specified in Chapter 3.3, "Technical data".
1.3 Instrument type
All information provided in these Instructions for Use applies only to the instrument type indi-
cated on the cover page. A nameplate is attached to the back of the instrument and a serial
number label is on the side of the instrument.
1. Important Information

9
HistoCore Arcadia H – Paraffin Embedding Station
These Instructions for Use include important instructions and information related to the
operating safety and maintenance of the instrument.
The Instructions for Use are an important part of the product, and must be read carefully prior
to startup and use and must always be kept near the instrument.
This instrument has been built and tested in accordance with the safety requirements for
electrical equipment for measurement, control, and laboratory use.
To maintain this condition and ensure safe operation, the user must observe all notes and
warnings contained in these Instructions for Use.
2. Safety
2.1 Safety notes
The safety and caution notes in this chapter must be observed at all times.
Be sure to read these notes even if you are already familiar with the operation and use
of other Leica Biosystems products.
These Instructions for Use must be appropriately supplemented as required by the
existing regulations on accident prevention and environmental safety in the operator's
country.
The protective devices on the instrument and its accessories must not be removed or
modified. Only service personnel qualified by Leica Biosystems may repair the instru-
ment and access the instrument's internal components.
Only use the provided power cable. This power cable must be replaced if the power
plug does not fit your countries socket. Please contact Leica Biosystems Service.
Residual risks
The instrument has been designed and constructed with the latest state-of-the-art tech-
nology and according to recognized standards and regulations with regard to safety
technology. Operating or handling the instrument incorrectly can place the user or other
personnel at risk of injury or can cause damage to the instrument or other property. The
instrument may be used only as intended and only if all of its safety features are in prop-
er working condition. Malfunctions that impede safety must be remedied immediately.
For current information about applicable guidelines, please refer to the CE declaration
of conformity and on our Internet site at:
http://www.LeicaBiosystems.com
The maximum load of the paraffin tank lid is 1kg. Do not exceed 1kg, otherwise damage
could occur to the paraffin tank lid.

10 Instructions for Use V 1.7 Rev. J – 12/2017
2. Safety
2.2 Warnings
The safety devices installed in this instrument by the manufacturer only constitute the basis
for accident prevention. Operating the instrument safely is, above all, the responsibility of the
owner, as well as the designated personnel who operate, service or repair the instrument.
To ensure trouble-free operation of the instrument, make sure to comply with the following
instructions and warnings.
Warnings – Safety notes on the instrument itself
Safety notes on the instrument itself, which are marked with a warning triangle,
indicate that the correct operating instructions (as defined in these Instructions for Use)
must be followed when operating or replacing the item marked. Nonobservance can
cause accidents, injuries and/or damage to the instrument/accessories.
Certain surfaces of the instrument are hot during operation under normal conditions.
They are marked with this warning sign. Touching these surfaces without suitable
safety measures can cause burns.
Safety instructions - Transport and installation
• After unpacking the instrument it may only be transported in an upright position.
• Place the instrument on a laboratory table and ensure it is level.
• The instrument must not be exposed to direct sunlight (window)!
• Only plug the instrument into a grounded power socket. If an extension cable has to
be used, make sure that it has a protective grounding conductor.
• Connect the instrument with the right power socket voltage 100~120V or 220~240V
according to the instrument type.
• The installation location must be well-ventilated; there should be no ignition sources
there of any kind.
• The instrument may not be operated in hazardous locations.
• Extreme temperature fluctuations between storage facility and setup site as well as
high humidity may cause condensation to form. In this case, wait at least two hours
before switching on.

11
HistoCore Arcadia H – Paraffin Embedding Station
2. Safety
Safety instructions - Working with the instrument
Paraffin is flammable and should therefore be handled with due care.
Do not use sharp tools to remove solidified paraffin from the work areas, as this may
destroy the coating on the surface. Use the plastic spatula supplied with the instrument.
During operation, the paraffin tank, embedding mold tray, cassette tray, work area as
well as the forceps holder are hot.
Risk of burning!
Do not move the instrument during operation.
Do not store any combustible and flammable substances near the instrument. There is
a fire hazard if work with an exposed flame (e.g. Bunsen burner) is carried out in the
direct vicinity of the instrument (solvent vapors). Therefore, keep all ignition sources at
least 2 meters away from the instrument!
Please allow 30 minutes before touching the instrument after shutting down.
Failure to observe the instructions specified by the manufacturer may result in damage
to the protection provided by the instrument.
Hazards - Servicing and cleaning
Switch off the instrument each time before servicing and pull out the power plug.
When using cleaners, please comply with the safety instructions of the manufacturer
and the laboratory safety regulations.
Before changing defective fuses, the instrument has to be disconnected from the power
supply. Fuses in the fuse holder on the back panel can be replaced by the user.
Liquid must not get into the instrument while operating or cleaning it.
2.3 Integrated safety devices
The instrument is equipped with the following safety features and devices:
Fuses in the heating elements
All of the instrument's AC heating elements are equipped with overheating fuses, which trip if
an AC heating element overheats and switches the element off.
Fuses will only automatically reset when the instrument is disconnected from AC power
and the heating element temperature drops below 50 °C.
Note the only way the user can disconnect the instrument from a power source is to
remove the power plug.
2.2 Warnings (Continued)

12 Instructions for Use V 1.7 Rev. J – 12/2017
3. Instrument Components and Specifications
3.1 Overview — Instrument components
Fig.2
13
9
1
3
6
2
11
4
8
5
7
10
Instrument front view
1. Power switch 6. Lid for left tray 11. Right tray
2. Control panel 7. Working surface 12. Work area illumination
3. Dispenser 8. Cold spot 13. Paraffin tank
4. Forceps holder 9. Paraffin collection trays
5. Left tray 10. Lid for right tray
12

13
HistoCore Arcadia H – Paraffin Embedding Station
3. Instrument Components and Specifications
3.1 Overview — Instrument components (Continued)
Instrument rear view
1
2
45Fig.3
3
3.2 Main features of the instrument
• Paraffin tank with a capacity of 4 litres.
• 5.7 inch LCD display and integrated capacitive touch keys.
• The paraffin flow is activated by means of a height-adjustable, pivotable clip - activated
either manually by pushing or via a foot switch (optional).
• Controllable flow rate.
• Removable paraffin collection trays.
• Spacious, easy-to-clean, heated work area, with integrated cold spot, also for extra large
cassettes ("Super Cassettes") with paraffin drain system.
• Trays for cassettes and/or molds with foldable lid, removable and interchangeable.
• Removable, heated forceps holder for 6 forceps, accessible from both sides.
• Optimum illumination of the working surface by LED lamp, controlled by the key on LCD
control panel.
• Temperature range of cassette and embedding mold tray, work area and paraffin tank
adjustable from 50 °C (122 °F) to 75 °C (167 °F).
• The beginning and end of the work time and work days can be programmed.
• Provide the error message for operation condition monitoring.
• Provide the enhance heating function for quicker paraffin melting.
1. Port for foot switch 3. Port for power inlet 5. Foot
2. Back panel 4. AC fuses

14 Instructions for Use V 1.7 Rev. J – 12/2017
3. Instrument Components and Specifications
3.3 Technical Data
General data
Voltage of power supply 100-120 VAC, 220-240 VAC, 50/60 Hz
Nominal current 10 A max.
Protection class 1I
Pollution degree 12
Over-voltage category II
Operating temperatures 50 °C (122 °F) to 75 °C (167 °F), adjustable in 1 °C
(or 1 °F) increments
IP protection class IP20
IP protection class (Foot switch) IPX8
Operating environment
Environmental operating temperature +20 °C to +30 °C
Environmental operating relative humidity 20 % to 80 % non-condensing
Environmental operating altitude Up to 2000 m
Transport and storage environment
Transport temperature -29 °C to +50 °C
Storage temperature +5 °C to +50 °C
Transport and storage relative humidity 10 % to 85 % non-condensing
1) according to IEC-61010, UL 3101, EN 61010
Electromagnetic environment Basic electromagnetic environment
Fuses
Time-lag fuses 5 x 20 mm 2 x T10A, 250 V
Dimensions and weights for instrument Order No. 14 0393 54090, 14 0393 54103, 14 0393 54100
Height 385 mm
Width 560 mm
Depth 600 mm
Weight 25 kg

15
HistoCore Arcadia H – Paraffin Embedding Station
3. Instrument Components and Specifications
Dimensions and weights for instrument Order No. 14 0393 57257, 14 0393 57258, 14 0393 57259
Height 384 mm
Width 560 mm
Depth 636 mm
Weight 27 kg
Capacities
Paraffin tank Max. 4 L
Programmable parameters
Temperature -Paraffin tank/dispenser
-Embedding mold tray/cassette tray,
-Work surface/forceps holder
Time -Working day, current weekday
-Working times (start, end), current time
3.3 Technical Data (Continued)

16 Instructions for Use V 1.7 Rev. J – 12/2017
• Stable, vibration-free laboratory table with horizontal, flat table top, as far as possible vi-
bration-free ground.
• The instrument must not be placed in the vicinity of the air outlet of an air conditioner and
must be shielded from strong sunlight (window).
• To ensure a fully functional heat sink, there must be gap of at least 15 cm behind the
instrument.
• The instrument must be installed in a place that ensures an easy disconnection from the
power supply. The power cable must be in a place that can be easily reached.
• The vicinity of the work area must be free of oil and chemical vapors.
4.1 Site requirement
The installation location must be well-ventilated and free of ignition sources of any
kind. The instrument should not be operated in hazardous locations.
4. Setting up the instrument

17
HistoCore Arcadia H – Paraffin Embedding Station
4.2 Standard delivery—packing list
Qty Designation Order No.
1 Basic unit HistoCore Arcadia H,
220-240 VAC 14 0393 54090
220-240 VAC, China 14 0393 54103
100-120 VAC 14 0393 54100
220-240 VAC 14 0393 57257
220-240 VAC, China 14 0393 57259
100-120 VAC 14 0393 57258
2 Left/right trays, removable 14 0393 53684
2 Lids for left/right trays 14 0393 53720
1 Paraffin scraper 14 0393 53643
1 Forceps holder, removable 14 0393 55225
1 Paraffin tank filter 14 0393 53559
4 Sets of spare fuses, 250 V 10 A 14 6000 04975
1 Instructions for Use (printed German/English
with language CD 14 0393 81200)
14 0393 81001
4. Setting up the instrument

18 Instructions for Use V 1.7 Rev. J – 12/2017
4. Setting up the instrument
Please compare the delivered components against the packing list, delivery note, and your
order. Should there be any discrepancy, please contact the Leica Biosystems distributor
handling your order.
4.2 Standard delivery—packing list (Continued)
The country specific power cord needs to be ordered separately. Please find a list of all power
cords available for your device on our website www.LeicaBiosystems.com within the product
section.