Leica VT1000 S User manual

Instructions for Use
VT1000 S
Vibrating-blade microtome
Leica VT1000 S, V 2.3 , English - 09/2018
Order No. 14 0472 80101 RevK
Always keep this manual with the instrument.
Read carefully before working with the instrument.

1
Leica VT1000 S - Vibrating-blade microtome
These are determined only by the contract
provisions agreed between ourselves and our
customers.
Leica reserves the right to change technical spec-
ifications as well as manufacturing processes
without prior notice. Only in this way is it pos-
sible to continuously improve the technology and
manufacturing techniques used in our products.
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Any copyrights of this document are retained by
Leica Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or of
any parts thereof) by means of print, photocopy,
microfiche, web cam or other methods – including
any electronic systems and media – requires
express prior permission in writing by Leica
Biosystems Nussloch GmbH.
For the instrument serial number and year of
manufacture, please refer to the nameplate at
the rear side of the instrument.
© Leica Biosystems Nussloch GmbH
The information, numerical data, notes and value
judgments contained in this manual represent
the current state of scientific knowledge and
state-of-the-art technology as we understand it
following thorough investigation in this field. We
are under no obligation to update the present
manual periodically and on an ongoing basis ac-
cording to the latest technical developments, nor
to provide our customers with additional copies,
updates etc. of this manual.
To the extent permitted in accordance with the
national legal system as applicable in each indi-
vidual case, we shall not be held liable for errone-
ous statements, drawings, technical illustrations
etc. contained in this manual.
In particular, no liability whatsoever is accepted
for any financial loss or consequential damage
caused by or related to compliance with state-
ments or other information in this manual.
Statements, drawings, illustrations and other
information as regards contents or technical
details of the present Instructions for Use are not
to be considered as warranted characteristics of
our products.
Note
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Phone: [phone]
Fax: [phone]
Internet: http://www.LeicaBiosystems.com
Assembly contracted to Leica Microsystems Ltd. Shanghai

2Instructions for Use, V 2.3 RevK – 09/2018
Contents
1. Important Information ....................................................................................................3
2. Safety ................................................................................................................................5
2.1 Safety notes................................................................................................................................... 5
2.2 Warnings........................................................................................................................................ 5
2.3 Safety instructions for handling the instrument...................................................................... 6
3. Instrument Characteristics ...........................................................................................7
3.1 Technical Data.............................................................................................................................. 7
3.2 General overview - VT1000 S...................................................................................................... 8
4. Installation.....................................................................................................................10
4.1 Standard delivery........................................................................................................................ 10
4.2 Unpacking and setting up the instrument............................................................................... 11
5. Operation........................................................................................................................13
5.1 Installation site requirements................................................................................................... 13
5.2 Setting up the instrument.......................................................................................................... 14
5.3 The operating elements and their function - VT1000 S ........................................................ 15
5.4 Adjusting the amplitude............................................................................................................. 19
5.5 Working with the VT1000 S on a daily basis........................................................................... 20
5.6 Routine daily maintenance and switching off the instrument - VT1000 S......................... 23
6. Malfunctions: Meaning and Troubleshooting.........................................................24
7. Cleaning and Maintenance ........................................................................................29
7.1 Cleaning the instrument............................................................................................................. 29
7.2 Changing the fuse....................................................................................................................... 30
8. Ordering Information: Spare Parts, Accessory, Consumables.............................31
8.1 Foot switch................................................................................................................................... 32
8.2 Buffer tray.................................................................................................................................... 32
8.2.1 Double-walled buffer tray S...................................................................................................... 32
8.3 Magnifier, LED illumination....................................................................................................... 33
8.4 Julabo recirculating cooler/chiller FL300............................................................................... 34
9. Warranty and Service..................................................................................................35
10. Decontamination Confirmation..................................................................................36

3
Leica VT1000 S - Vibrating-blade microtome
Symbols used in this manual and their meaning
1. Important Information
(5)
(Fig. 5)
Numbers in parentheses refer to item num-
bers in illustrations or to the illustrations
themselves.
Useful tips,
i.e. important information for the user,
appear in gray boxes and are marked by
an .
Dangers, warnings and cautions
appear in a gray box and are marked by
a warning triangle .
Environmental protection symbol of the
China RoHS directive.
The number in the symbol indicates the
"Environment-friendly Use Period" of the
product in years.
The symbol is used if a substance
restricted in China is used in excess of
the maximum permitted limit.
The CE labeling shows that the product
corresponds to one or more applicable
European directives.
Symbol for labeling electrical and elec-
tronic equipment in accordance with
Section 7 of the German Electrical and
Electronic Equipment Act (ElektroG).
ElektroG is the law on the bringing into
circulation, return and environmentally
compatible disposal of electrical and
electronic equipment.
Caution! Follow the accompanying
documentation!
Instrument model:
All information provided in these Instructions for
Use applies only to the instrument type indicated
on the title page. A nameplate indicating the
instrument serial number is attached to the rear
side of the instrument.
Information:
When making inquiries, please specify correctly:
• Instrument model • Serial number
Observe the Instructions for Use
Order No.
Serial number
Date of Manufacture
Manufacturer
The Regulatory Compliance Mark (RCM)
indicates a device’s compliance with
applicable ACMA technical standards of
New Zealand and Australia - that is, for
telecommunications, radio communica-
tions, EMC and EME.

4Instructions for Use, V 2.3 RevK – 09/2018
Qualification of personnel
The Leica VT1000 S should be operated by trained laboratory personnel only.
All laboratory personnel designated to operate this instrument must read these Instructions for
Use carefully and must be familiar with all technical features of the instrument before attempt-
ing to operate it.
Intended use/improper use
The VT1000 S is used for sectioning in the fields of medicine, biology and industry, and is espe-
cially designed for sectioning fixed or unfixed fresh tissue in a buffer solution.
The VT1000 S may be used for research purposes only. Sections made using the VT1000 S
must NOT be used for diagnostics!
The instrument must be used exclusively according to the instructions contained in these
Instructions for Use.
Any other use of the instrument is considered improper.
1. Important Information

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Leica VT1000 S - Vibrating-blade microtome
2. Safety
2.1 Safety notes
These Instructions for Use includes important
information related to the operating safety and
maintenance of the instrument.
The Operating Manual is an important part of
the product, and must be read carefully prior to
startup and use and must always be kept near
the instrument.
This instrument has been built and tested in
accordance with the safety requirements for
electrical equipment for measurement, control,
and laboratory use.
To maintain this condition and ensure safe opera-
tion, the user must observe all notes and warnings
contained in these Instructions for Use.
The current EC Declarations of Conformity can be
found on the Internet:
www.LeicaBiosystems.com
2.2 Warnings
The safety devices installed in this instrument by the manufacturer only constitute the basis
for accident prevention. Operating the instrument safely is, above all, the responsibility of the
owner, as well as the designated personnel who operate, service or clean the instrument.
To ensure trouble-free operation of the instrument, make sure to comply with the following
instructions and warnings.
These Instructions for Use must be ap-
propriately supplemented as required
by the existing regulations on accident
prevention and environmental safety in
the operator‘s country.
The safety and caution notes in this chapter must be observed at all times.
Be sure to read these notes even if you are already familiar with the operation and use of
other Leica products.
The protective devices located on the instrument and the accessories must not be
removed or modified. The instrument must only be opened and repaired by service techni-
cians authorized by Leica.

6Instructions for Use, V 2.3 RevK – 09/2018
2. Safety
2.3 Safety instructions for handling the instrument
Proper handling
Be sure to handle knives and blades very cau-
tiously!
Never touch the cutting edge of knives and
blades!
Do not leave knives, blades and bladed knife
holders unprotected.
All appropriate safety precautions must be met
to avoid the risk of infection.
Protective clothing according to safety regula-
tions for "Working with harmful substances"
(Safety mask, gloves, protective clothing) must
be worn!
Cover the magnifier during work breaks as it may
act as a burning glass when not covered!
In case of emergency, press the red EMERGENCY
STOP switch (at the right side of the instrument).
To release the switch, turn it in the direction of
the arrow.
The instrument may be opened by authorized
service personnel only.
Before removing the cover, ensure that the instru-
ment is unplugged.
Warning: Avoid touching live parts
under any circumstances!
Danger
Caution: Extremely sharp blades pose
risk of injury when touched!
Caution: Fresh tissue poses risk of
infection!
Caution: When not in use, cover magni-
fier with corresponding lid to avoid risk
of fire.

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Leica VT1000 S - Vibrating-blade microtome
3. Instrument Characteristics
3.1 Technical Data
General data:
Sectioning frequency (± 10 %).............................................................................................................0 - 100 Hz
Amplitude.................................................................................... adjustable in 5 steps: 0.2; 0.4; 0.6; 0.8; 1 mm
Sectioning speed (± 10 %) ........................................................................................................ 0.025 - 2.5 mm/s
Return stroke speed (± 10 %) ...................................................................................................................5 mm/s
Total vertical specimen stroke............................................................................................15 mm (motorized)
Sectioning range ............................................................................................................ 1 - 40 mm (adjustable)
Specimen retraction .................................................................0 - 999 µm (adjustable; can be deactivated)
Maximum specimen size:
with standard knife holder.......................................................................................................... 33 x 40 mm
Specimen orientation.....................................................................................................................................330°
Section thickness selection......................................................................................1 - 999 µm, in 1-µm steps
Magnifier, assy. (standard accessory of the configured instrument)............................ 2 x magnification
Ambient conditions:
Operating temperature range....................................................................................... min. 5 °C to max. 40 °C
Relative humidity .................................................................................................................................. max. 80 %
Height: .................................................................................................................. up to 2000 m above sea level
Electrical data:
Rated voltage range (± 10 %): ........................................................................................................ 100 V - 240 V
Nominal frequency (± 10 %): ................................................................................................................50 - 60 Hz
Power draw .................................................................................................................................................. 50 VA
Mains fuse .................................................................................................................................................T 1.25 A
Pollution degree....................................................................................................................................................2
Overvoltage installation category..................................................................................................................... II
Overload protection ........................................................................................................................................ yes
Internal current limiter of electronics.......................................................................................................... yes
Dimensions:
L x W x H...............................................................................................................480 mm x 360 mm x 200 mm
Height with magnifier support................................................................................................................ 285 mm
Weight:
(without magnifier support) ........................................................................................................................ 17 kg
(magnifier support only) ................................................................................................................................ 2 kg
(total)............................................................................................................................................................... 19 kg

8Instructions for Use, V 2.3 RevK – 09/2018
3. Instrument characteristics
3.2 General overview - VT1000 S
Buffer tray S
EMERGENCY STOP
switch (not visible here)
Fixture for
magnifier
support
Module LED Hi-Power spots, 2-arm
(optional)
Magnifier
Module Hi-Power spot, LED 1000
(optional)
Knife holder
Control panel
Magnifier support
Cutting head
Fig. 2