Leica HistoCore Water Bath User manual

HistoCore Water Bath
Water Bath
Instructions for Use
English
Order No.: 14 0607 80101 - Revision A
Always keep this manual with the instrument.
Read carefully before working with the instrument.
Version 1.0, Revision A - 09.2022

3
HistoCore Water Bath
The information, numerical data, notes and value judgments contained in this Instructions for Use
represent the current state of scientic knowledge and state-of-the-art technology as we understand it
following thorough investigation in this eld.
We are under no obligation to update the present Instructions for Use periodically and on an ongoing
basis according to the latest technical developments, nor to provide our customers with additional
copies, updates etc. of this Instructions for Use.
To the extent permitted in accordance with the national legal system as applicable in each individual
case, we shall not be held liable for erroneous statements, drawings, technical illustrations etc. contained
in this Instructions for Use. In particular, no liability whatsoever is accepted for any nancial loss or
consequential damage caused by or related to compliance with statements or other information in this
Instructions for Use.
Statements, drawings, illustrations and other information regarding the contents or technical details of
the present Instructions for Use are not to be considered warranted characteristics of our products.
These are determined only by the contract provisions agreed between ourselves and our customers.
Leica reserves the right to change technical specications as well as manufacturing processes without
prior notice. Only in this way is it possible to continuously improve the technology and manufacturing
techniques used in our products.
This document is protected under copyright laws. All copyrights to this documentation are held by Leica
Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or of any parts thereof) by means of print, photocopy,
microche, web cam or other methods – including any electronic systems and media – requires express
prior permission in writing by Leica Biosystems Nussloch GmbH.
For the instrument serial number and year of manufacture, please refer to the nameplate on the back of
the instrument.
Leica Biosystems Nussloch GmbH
Heidelberger Strasse 17 - 19
69226 Nussloch
Germany
Tel.: +49 - (0) 6224 - 143 0
Fax: +49 - (0) 6224 - 143 268
Web: www.LeicaBiosystems.com
Assembly contracted to Leica Microsystems Ltd. Shanghai
Note

4Version 1.0, Revision A
Table of contents
1. Important information ..................................................................................................5
1.1 Naming conventions .................................................................................................................. 5
1.2 Symbols and their meanings....................................................................................................... 5
1.3 Instrument type ......................................................................................................................... 8
1.4 Intended purpose....................................................................................................................... 8
1.5 Qualicationofpersonnel .......................................................................................................... 8
2. Safety........................................................................................................................9
2.1 Safety notes .............................................................................................................................. 9
2.2 Warnings..................................................................................................................................10
2.2.1 Markings on the instrument itself ..............................................................................................10
2.2.2 Transport and installation..........................................................................................................10
2.2.3 Operating the instrument...........................................................................................................11
3. Instrumentcomponentsandspecications.................................................................... 12
3.1 Overview ..................................................................................................................................12
3.1.1 Instrument components ............................................................................................................12
3.1.2 Rear view..................................................................................................................................12
3.2 Main features of the instrument.................................................................................................13
3.3 Technical data ..........................................................................................................................13
4. Setting up the instrument ........................................................................................... 15
4.1 Installation site requirements ....................................................................................................15
4.2 Standard delivery-packing list....................................................................................................15
4.3 Unpacking the instrument..........................................................................................................16
4.4 Setting up the instrument ..........................................................................................................17
4.5 Switching on/off the instrument ................................................................................................19
4.6 Moving the instrument ..............................................................................................................19
5. Operation ................................................................................................................. 21
5.1 Control panel on the instrument.................................................................................................21
5.2 Setting the temperature.............................................................................................................22
5.3 Power on/off the slide dryer ......................................................................................................23
6. Cleaning and maintenance .......................................................................................... 24
6.1 Cleaning the instrument ............................................................................................................24
6.2 Replacing fuses ........................................................................................................................24
7. Troubleshooting ........................................................................................................ 25
8. Ordering information.................................................................................................. 26
A1. DecontaminationConrmation .................................................................................... 27
A2. Warranty and Service ................................................................................................. 28

5
HistoCore Water Bath
1Important information
1. Important information
1.1 Naming conventions
Note
• The full name of the device is HistoCore Water Bath. The device is called the water bath to ensure
that the Instructions for Use are well legible.
1.2 Symbols and their meanings
Symbol: Title of the symbol: Danger
Description: Indicates an imminently hazardous situation which, if not
avoided, will result in death or serious injuries.
Symbol: Title of the symbol: Warning
Description: If this danger is not avoided, then this may result in death
or serious injury.
Symbol: Title of the symbol: Caution
Description: Indicates a potentially hazardous situation which, if not
avoided, could result in death or serious injury.
Symbol: Title of the symbol: Note
Description: Indicates information which is important but not related to
any risk.
Symbol: Title of the symbol: Item number
→Fig. 7-1Description: Item numbers for numbering illustrations. Numbers in red
refer to item numbers in illustrations.
Symbol: Title of the symbol: Function key
OFF Description: Function keys to be pressed on the instrument are
displayed as bold, gray and underlinded text.
Symbol: Title of the symbol: Manufacturer
Description: Indicates the manufacturer of the product.
Symbol: Title of the symbol: Manufacturing date
Description: Indicates the date when the device was manufactured.
Symbol: Title of the symbol: Article number
Description: Indicates the manufacturer's catalog number so that the
devicecanbeidentied.
Symbol: Title of the symbol: Serial number
Description: Indicates the manufacturer's serial number so that a
specicdevicecanbeidentied.

6Version 1.0, Revision A
1Important information
Symbol: Title of the symbol: Consult Instructions for Use
Description: Indicates the need for the user to consult the Instructions
for Use.
Symbol: Title of the symbol: Caution
Description: Indicates the need for the user to consult the Instructions
for Use for important cautionary information such as
warnings and precautions that cannot, for a variety of
reasons, be presented on the device itself.
Symbol: Title of the symbol: Country of Origin
Description: TheCountryofOriginboxdenestheCountrywherethe
nalcharactertransformationoftheproducthasbeen
performed.
Symbol: Title of the symbol: CE Compliance
Description: The CE marking is the manufacturer's declaration that
the product meets the requirements of the applicable EC
directives and regulations.
Symbol: Title of the symbol: UKCA
Description: The UKCA (UK Conformity Assessed) marking is a new UK
product marking that is used for goods being placed on
the market in Great Britain (England, Wales and Scotland).
It covers most goods which previously required the CE
marking.
Symbol: Title of the symbol: UKRP
Description: The UK Responsible Person acts on behalf of the non-UK
manufacturertocarryoutspeciedtasksinrelationtothe
manufacturer’s obligations.
Symbol: Title of the symbol: WEEE Symbol
Description: The WEEE symbol, indicating separate collection for
WEEE–Wasteofelectricalandelectronicequipment,
consists of the crossed-out wheeled bin (§ 7 ElektroG).
Symbol: Title of the symbol: China ROHS
Description: Environmental protection symbol of the China RoHS
directive. This symbol means the device doesn't contain
harmful or hazardous substance or elements.
Symbol: Title of the symbol: Alternating current
Symbol: Title of the symbol: PE terminal
Symbol: Title of the symbol: ON (Power)
Description: In the position of power on

7
HistoCore Water Bath
1Important information
Symbol: Title of the symbol: OFF (Power)
Description: In the position of power off
Symbol: Title of the symbol: Caution, hot surface
Description: Instrument surfaces which become hot during operation
are marked with this symbol. Avoid direct contact to
prevent the risk of burning.
Symbol: Title of the symbol: Caution, risk of electric shock
Description: If the AC outlet connector is wrongly operated, there will
be a risk of electric shock. Avoid any wrong operation.
Symbol: Title of the symbol: Fragile, handle with care
Description: The package contents are fragile and must be handled
with care.
Symbol: Title of the symbol: Keep dry
Description: The package must be kept in a dry environment.
Symbol: Title of the symbol: Stack limit
Description: The largest number of identical packages allowed to be
stacked; "6" stands for the number of permitted packages.
Symbol: Title of the symbol: This way up
Description: Indicates the correct upright position of the package.
Symbol: Title of the symbol: Temperature limit for transport
Description: Indicates the temperature range permitted for transporting
the package.
Symbol: Title of the symbol: Temperature limit for storage
Description: Indicates the temperature range permitted for storing the
package.
Symbol: Title of the symbol: Humidity limitation for transport and storage

8Version 1.0, Revision A
1Important information
Description: Indicates the humidity range permitted for storing and
transporting the package.
Symbol: Title of the symbol: Recycle symbol
Description: Indicates the item can be recycled where correct facilities
exist.
1.3 Instrument type
All information provided in these Instructions for Use applies only to the instrument type indicated on
the cover page. A nameplate indicating the instrument serial number is attached to the rear side of the
instrument.
1.4 Intended purpose
Theintendeduseoftheproductistoprovidewaterbathtohelpattentheoatingslidesribbonandalso
a slide dryer to help dry the water on the slides. It could be used by biomedical research institute.
Warning
• Any other use of the instrument is considered Off Label use. Failure to observe these instructions
may result in an accident, personal injury, damage to the instrument, accessories, or specimens.
Proper and intended use includes compliance with all inspection and maintenance instructions,
along with the observance of all instructions in the Instructions for Use.
1.5 Qualicationofpersonnel
• The HistoCore Water Bath must be operated by trained laboratory personnel only. The instrument is
intended for professional use only.
• All laboratory personnel designated to operate this instrument must read these Instructions for Use
carefully and must be familiar with all technical features of the instrument before attempting to
operate it.

9
HistoCore Water Bath
Safety 2
2. Safety
2.1 Safety notes
The Instructions for Use includes important information related to the operating safety and maintenance
of the instrument.
The Instructions for Use is an important part of the product, and must be read carefully prior to startup
and use, and must always be kept near the instrument.
This instrument has been built and tested in accordance with the safety requirements for electrical
equipment for measurement, control, and laboratory use.
To maintain this condition and ensure safe operation, the user must observe all notes and warnings
contained in the Instructions for Use.
The safety and caution notes in this chapter must be observed at all times. Be sure to read these notes
even if you are already familiar with the operation and use of other Leica Biosystems products.
The Instructions for Use must be appropriately supplemented as required by the existing regulations on
accident prevention and environmental safety in the operator‘s country.
Warning
• Theprotectivedeviceslocatedontheinstrumentandtheaccessoriesmustnotberemovedormodied.
OnlyqualiedservicepersonnelauthorizedbyLeicaBiosystemsmayrepairtheinstrumentandaccess
its internal components.
• If the instrument is to be returned to Leica Biosystems for repair, it must be cleaned and decontaminated
in the appropriate manner (→p.27–A1.DecontaminationConrmation).
Note
For current information about applicable standards, please refer to the CE Declaration of Conformity
andUKCACerticatesonourInternetsite:
http://www.LeicaBiosystems.com
• Before connecting the instrument to the line voltage, ensure that the electrical power requirements of
your laboratory match the values on the nameplate of the instrument.
• When installing the power cable, always be sure to route it so that it cannot contact the heated
surfaces of the instrument at any time.
• The instrument is designed for indoor use only.
• The instrument must be switched off and unplugged from the power supply during all repair and
service work.
• During operation, the instrument surface can be very hot.

10 Version 1.0, Revision A
Safety
2
2.2 Warnings
The safety devices installed in this instrument by the manufacturer only constitute the basis for accident
prevention. Operating the instrument safely is, above all, the responsibility of the owner, as well as the
designated personnel who operate, service or repair the instrument.
To ensure trouble-free operation of the instrument, make sure to comply with the following instructions
and warnings.
Please note that this instrument is designed to be used in basic electromagnetic environment.
Please note that electrostatic charge may result from direct or indirect contact with the instrument.
2.2.1 Markings on the instrument itself
Warning
Failuretofollowthecorrectoperatinginstructions(asdenedintheInstructionsforUse)ofthe
corresponding markings and warning triangles on the instrument.
Serious injury of persons and/or damage to instrument, accessories, or specimens.
• Pay attention to the markings on the instrument and strictly follow operating instructions described
in the Instructions for Use when operating or replacing the item marked.
2.2.2 Transport and installation
Danger
Hazardoussubstances(combustibleorammable)arestoredneartheinstrumentorlledinthewater
tray.
People injury due to the explosion or ignition of the hazardous substances.
• Donotstorecombustibleorammablesubstancesneartheinstrument.
• Usenon-ammableliquid(preferablydistilledwater)only.
Warning
The water bath or slide dryers fall off while being lifted by the user.
The instrument or accessories fall or drop to the user, which leads to people injury.
The user prepares sections with potentially damaged instrument or accessories, which may lead to
tissue damage.
• Be careful when lifting devices.
Warning
Move the water bath with the slide dryer connected.
Connection cable is damaged, and the slide dryer falls onto the user.
• Disconnect the slide dryer from the water bath when attempting to move the water bath.
Warning
Move the water bath with heated water inside.
Water splashes out, and people slip. Or heated water hurts the user.
• Stop heating and empty the instrument before moving.

11
HistoCore Water Bath
Safety 2
Warning
The user plugs the instrument onto a power board with other instruments.
The instrument malfunctions due to unstable current/power, which leads to tissue damage.
• Do not use a power board to install the power cable.
• The instrument must be connected to a grounded power socket.
2.2.3 Operating the instrument
Warning
Theuseraccidentallygetscontactwithheatedwaterwhenoatingorpickingupsections.
The user gets hurt by hot water.
• Be careful of the hot water during operation.
Warning
The user accidentally gets contact with heated surface of the slide dryer.
The user gets hurt by the heated slide dryer.
• Be careful of the hot surface of the slide dryer during operation.
Warning
The water bath base is exposed when the water tray is taken away for exchange of water.
The heated surface of the water bath base hurts the user.
• Be careful of the hot surface of the water bath base during exchange of water.

12 Version 1.0, Revision A
Instrumentcomponentsandspecications
3
3. Instrumentcomponentsandspecications
3.1 Overview
3.1.1 Instrument components
Fig. 1
1
3
2
4
1 Water bath 3 Control panel
2 Water tray 4 Slide dryer (ordered separately)
3.1.2 Rear view
Fig. 2
13
2
4
5
6
1 Mains power inlet 4 LED illumination
2 Cable connectors 5 Heating sensor (under the water tray)
3 Two fuses 6 Water slot (under the water tray)

13
HistoCore Water Bath
Instrumentcomponentsandspecications 3
3.2 Main features of the instrument
HistoCore Water Bath:
• Detachable water tray;
• Black water tray to provide good visibility and contrast;
• Auxiliary illumination to help with visibility and contrast;
• Anodizing treatment to improve coating durability,
• OLED touch panel to control and indicate both water bath and slide dryer;
• Intuitive user interface to make it easy to access and easy to learn;
• Dimension:280mmx280mmx105mm;
• Heating temperature: up to 60°C.
HistoCore Slide Dryer:
• Space-saving design, small footprint;
• Up to 30 slide capacity;
• Black rack to provide good visibility and contrast;
• 45° angeled heated rack, easy for slides pick up and placement;
• Accessory to HistoCore Water Bath;
• No need for power socket, get power supply via HistoCore Water bath;
• Dimension:200mmx280mmx98mm;
• Heating temperature: up to 75°C.
3.3 Technical data
Equipmentidentication
Model name HistoCore Water Bath
Model number(s) 140607020C0 (Water Bath),
140607010C0 (Optional accessory: Slide Dryer)
Electricalspecications
Nominal supply voltage 100-120VAC/220-240VAC
Nominal supply frequencies 50/60Hz
Mainssupplyvoltageuctuations +/-10%
Power consumption • 960W max at 100-120VAC
• 1200W max at 220-240VAC
Main input fuses (F1) 10A 250VAC
Dimensionsandweightspecication
Overall size of device in operating mode
(Width x Depth x Height, mm)
• Water bath: 280 x 280 x 105
• Slide dryer: 200 x 280 x 98
Overall size serial packaging
(Width x Depth x Height, mm)
415 x 395 x 215
Empty weight (without accessories, kg) 3
Overall weight (with accessories, kg) 3
Device weight including packaging (kg) 4

14 Version 1.0, Revision A
Instrumentcomponentsandspecications
3
Environmentalspecication
Operating altitude
(meters above sea level) (min/max)
Up to 2000 m
Temperature (operation) (min/max) 15 - 40°C
Relative humidity (operation) (min/max) 20% - 80%
Temperature (transit) (min/max) -29°C - 50°C
Temperature (storage) (min/max) 5°C - 50°C
Relative humidity (transit/storage) 20% - 85%
Minimum distance to walls (mm) 10 cm
BTU (J/s) 4094 BTU/h
Emissions and boundary conditions
Overvoltage category to IEC 61010-1 II
Pollution degree to IEC 61010-1 2
Means of protection to IEC 61010-1 I
Degree of protection to IEC 60529 IP20
Heat emission <4094 BTU/h
EMC class Class B
Electrical connections and interfaces
Mains power supply *1 100-120V 8A max/220-240V 5A max
Signal connector to Slide Dryer *2 3.3V DC max
Power supply to Slide Dryer *2 100-120V 4A max/220-240V 2A max (not labeled)
Otherspecications
CE Approved

15
HistoCore Water Bath
Setting up the instrument 4
4. Setting up the instrument
4.1 Installation site requirements
Danger
Hazardoussubstances(combustibleorammable)arestoredneartheinstrumentorlledinthewater
tray.
People injury due to the explosion or ignition of the hazardous substances.
• Donotstorecombustibleorammablesubstancesneartheinstrument.
• Usenon-ammableliquid(preferablydistilledwater)only.
• When installing the power cable, always be sure to route it so that it cannot contact the heated
surfaces of the instrument at any time.
• The power socket to which the instrument is to be connected to has to be located near the instrument
and easily accessible.
• Thepowersupplymustbeatadistancenogreaterthanthelengthofthepowercable–anextension
cable must not be used.
• Thesubstratemustbelargelyvibration-freeandhavesucientloadcapacityandrigidityfortheweight
of the instrument.
• Avoidimpacts,brightdirectlight,andexcessivetemperatureuctuations.
• The instrument must be connected to a suitable power socket. Use only the provided power cable,
which is intended for the local power supply.
4.2 Standard delivery-packing list
Quantity Part description Order No.
1 HistoCore Water Bath 14 0607 020C0
1 basic instrument 14 0607 02000
1 Set of fuses 14 6000 05950
1 Instructions for Use (printed in English with additional languages on
data carrier 14 0607 80200)
14 0607 80001
If the supplied local power cord is defective or lost, please contact your local Leica representative.
Note
• Please check all delivered parts against the packing list and against your order to verify whether
thedeliveryiscomplete.Shouldyoundanydiscrepancies,pleasecontactyourLeicaBiosystems
salesocewithoutdelay.

16 Version 1.0, Revision A
Setting up the instrument
4
4.3 Unpacking the instrument
1. Setthecartononaatsurface,cutthroughtheadhesivetapeandopenthecarton.
Fig. 3
2. Remove the accessories and carefully lift the instrument out of the carton.
Fig. 4
3. Remove the plastic cover.
Note
• The transport carton and included retaining elements should be kept in case a return shipment is
necessary later. To return the instrument, follow the instructions above in reverse order.

17
HistoCore Water Bath
Setting up the instrument 4
4.4 Setting up the instrument
The water bath can work alone, or work with up to two slide dryers.
Fig. 5
The water bath works alone.
Fig. 6
The slide dryer is connected at the right side.
Fig. 7
The slide dryer is connected at the left side.
Fig. 8
The slide dryer is connected at the rear end.
Fig. 9
Two slide dryers are connected at the right side in
parallel.
Fig. 10
Two slide dryers are connected at the left side in
parallel.
Fig. 11
One slide dryer is connected at the right side, and
the other one is at the rear end.
Fig. 12
One slide dryer is connected at the left side, and
the other one is at the rear end.

18 Version 1.0, Revision A
Setting up the instrument
4
Connecting the slide dryer(s) to the water bath
1. Remove the corresponding covers (→Fig. 13-1)on the bottoms of the wather bath and the
slide dryer by loosening screws (→Fig. 13-2).
Fig. 13
1
2
2. Pull out the cables from two devices. Connect the cables together (→Fig. 14-1). You will hear a
clicksoundandfeeltheconnectorsttingintoeachother.Iftwoslidedryersaretobeinstalled
in parallel at the same side of the water bath, use the extension cable to connect the water bath
and the outer slide dryer (→Fig. 14-2).
Fig. 14
12
Setting up the power supply
1. Before connecting the power cable, make sure that the main switch (→Fig. 15-1)on the front
of the instrument is at "O" (OFF) position.
Fig. 15 1

19
HistoCore Water Bath
Setting up the instrument 4
2. Use the instrument with the provided power cable only. Insert the connector of the power cable
into the connection socket (→Fig. 16-1)and plug the power plug into the power socket.
Fig. 16 1
4.5 Switching on/off the instrument
Warning
The user plugs the instrument onto a power board with other instruments.
The instrument malfunctions due to unstable current/power, which leads to tissue damage.
• Do not use a power board to install the power cable.
• The instrument must be connected to a grounded power socket.
Switching on the instrument
1. Beforeswitchingtheinstrumenton,llthewaterbathwithasucientquantityofdistilledwater.
2. Switch on the instrument with the main switch at the front right corner of the instrument. The
run/stop button on the control panel is illuminated.
3. Touch the run/stop button, the heating to the water bath and slide dryer(s) (if connected) starts.
For the buttons' functions on the control panel, go to (→p.21–5.1Controlpanelontheinstrument).
Switching off the instrument
The instrument is switched off by single touch of the run/stop button.
You do not have to switch the main switch on or off each time for everyday routine use.
4.6 Moving the instrument
The instrument must be switched off and disconnected from the power supply. The water tray must be
empty. The instrument must have cooled down before moving.

20 Version 1.0, Revision A
Setting up the instrument
4
Warning
The water bath or slide dryers fall off while being lifted by the user.
The instrument or accessories fall or drop to the user, which leads to people injury.
The user prepares sections with potentially damaged instrument or accessories, which may lead to
tissue damage.
• Be careful when lifting devices.
Warning
Move the water bath with the slide dryer connected.
Connection cable is damaged, and the slide dryer falls onto the user.
• Disconnect the slide dryer from the water bath when attempting to move the water bath.
Warning
Move the water bath with heated water inside.
Water splashes out, and people slip. Or heated water hurts the user.
• Stop heating and empty the instrument before moving.