Optika Italy ECOVISION Series User manual

Ver. 2.1 2022
Model
B-20R
B-20CR
M-100FX
M-100FLed
ECOVISION Series
INSTRUCTION MANUAL

Page 2
Table of contents
1. Warning 3
2. Safety Information 3
3. Unpacking 3
4. Intended use 3
5. Symbols and conventions 3
6. Instrument description 4
6.1 B-20R 4
6.2 B-20CR 5
6.3 M-100FX 6
6.4 M-100FLed 7
7. Assembling 8
7.1 Microscope assembling 8
7.1.1 Installing the batteries (M-100FLed) 8
7.1.2 Installing the batteries (B-20R / B-20CR) 8
8. Use of the microscope 9
8.1 Switching on the microscope 9
8.1.1 B-20R / B-20CR / M-100FX 9
8.1.2 M-100FLed 9
8.2 Light intensity adjustment 9
8.2.1 M-100FX 9
8.2.2 B-20R / B-20CR / M-100FX 9
8.2.3 M-100FLed 9
8.3 Placing the specimen 10
8.3.1 B-20R / M-100FX / M-100FLed 10
8.3.2 B-20CR 10
8.4 Use with batteries 10
8.4.1 M-100FLed 10
8.4.2 B-20R / B-20CR 10
8.5 Aperture diaphragm 11
9. Maintenance 12
10. Troubleshooting 13
Equipment disposal 14

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It
is built to high optical and mechanical standards and to withstand daily use. We remind you that this manual contains
important information on safety and maintenance, and that it must therefore be made accessible to the instrument users.
We decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.
2. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the operation voltage
of the equipment and that the lamp switch is in off position. Users should observe all safety regulations of the region. The
equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please
follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.
3. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container and lift the
top half of the container. Take some care to avoid that the optical items (objectives and eyepieces) fall out and get dam-
aged. Using both hands (one around the arm and one around the base), lift the microscope from the container and put it
on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other
residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the subject.
5. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.

Page 4
OBJECTIVE
NOSEPIECE
STAGE
EYEPIECE
FINE FOCUSING KNOB
COARSE
FOCUSING KNOB
MAIN BODY
ILLUMINATOR
LOCK SCREW
OBSERVATION HEAD
CARRYING HANDLE
SLIDE CLAMP
CONDENSER
6. Instrument description
6.1 B-20R

Page 5
NOSEPIECE
OBJECTIVE
STAGE
STAGE KNOBS
CONDENSER
EYEPIECE
COARSE FOCUSING
KNOB
FINE FOCUSING
KNOB
MAIN BODY
APERTURE
DIAPHRAGM
ILLUMINATOR
LOCK SCREW
OBSERVATION HEAD
CARRYING HANDLE
SLIDE CLAMP
6.2 B-20CR

Page 6
OBJECTIVE
CONDENSER
ILLUMINATOR
EYEPIECE
MAIN BODY
OBSERVATION
HEAD
FINE FOCUSING
KNOB
COARSE
FOCUSING KNOB
STAGE
SLIDE CLAMP
NOSEPIECE
6.3 M-100FX
APERTURE
DIAPHRAGM
LOCK SCREW

Page 7
OBJECTIVE
ILLUMINATOR
CONDENSER
ON-OFF /
BRIGHTNESS
ADJUSTMENT KNOB
EYEPIECE
MAIN BODY
OBSERVATION
HEAD
FINE FOCUSING
KNOB
COARSE
FOCUSING KNOB
STAGE
SLIDE CLAMP
NOSEPIECE
6.4 M-100FLed
APERTURE
DIAPHRAGM
LOCK SCREW

Page 8
7. Assembling
FFig. 1ig. 1
FFig. 2ig. 2
FFig. 4ig. 4
FFig. 3ig. 3
7.1 Microscope assembling
The microscope comes already assembled from the factory. No
assembling procedure is requested.
7.1.1 Installing the batteries (M-100FLed)
• It is necessary to use 3x AA rechargeable NiMh 1.2V.
1. Remove the cover (Fig. 1) and insert the batteries (not pro-
vided), respecting the polarity. (Fig. 2)
2. Put back the battery compartment cover.
7.1.2 Installing the batteries (B-20R / B-20CR)
• It is necessary to use 3x AAA rechargeable NiMh 1.2V.
1. Remove the cover and insert the batteries (not provided), re-
specting the polarity. (Fig. 3)
2. Put back the battery compartment cover.

Page 9
8. Use of the microscope
8.1 Switching on the microscope
8.1.1 B-20R / B-20CR / M-100FX
1. Operate on the main switch ① placed in the rear side of the
microscope, moving the selector on “I”. (Fig. 4)
FFig. 4ig. 4
FFig. 5ig. 5
FFig. 6ig. 6
③
①
②
8.1.2 M-100FLed
1. Operate on the main switch ② placed in front of the micro-
scope. (Fig. 5)
8.2 Light intensity adjustment
8.2.1 M-100FX
• No light intensity adjustment can be done on this model.
8.2.2 B-20R / B-20CR / M-100FX
1. Turn the light intensity dial ③ placed on the right side of the
microscope. (Fig. 6)
8.2.3 M-100FLed
1. Operate on the main switch ② placed in front of the micro-
scope. (Fig. 5)

Page 10
8.3 Placing the specimen
8.3.1 B-20R / M-100FX / M-100FLed
1. Place the specimen on the stage and secure it using the
stage clips ①. (Fig. 7).
FFig. 7ig. 7
FFig. 9ig. 9
③
①
FFig. 8ig. 8
②
8.3.2 B-20CR
Stage accepts standard slides 26 x 76 mm, thickness 1,2 mm
with coverslide 0,17 mm. (Fig. 8)
1. Open the spring arm of the slide holder ② and place the slide
from the front on the stage.
2. Gently release the spring arm of the slide holder.
• A sudden release of the spring arm could cause the fall-
ing of the slide.
8.4 Use with batteries
8.4.1 M-100FLed
• The microscope is provided with an ON-OFF switch ③ (Fig.
9) placed on the bottom side of the microscope. The use of
the switch is as follows:
1. Switch placed in “OFF” position: use of the microscope
is possible only with external power supply. Battery use and
recharge is disabled.
2. Switch placed in “ON” position: use of the microscope is
possible either with external power supply or with batteries.
In this case the battery recharge is enabled.
• Once the batteries are charged, it is recommended to
disconnect the power supply, or set the switch to “OFF”.
8.4.2 B-20R / B-20CR
• The batteries recharge when the power supply is connected
to the power outlet. Unplugging the power supply starts the
microscope’s battery operation.
• Once the batteries are charged, it is recommended to
disconnect the power supply.

Page 11
8.5 Aperture diaphragm
• The Numerical Aperture (N.A.) value of the aperture dia-
phragm affects the image contrast. Increasing or reducing
this value one can vary resolution, contrast and depth of fo-
cus of the image. Move the diaphragm ring ① (Fig. 10) on the
value corresponding to the objective in use. In this case the
optimal setting of the condenser is achieved.
• With low contrast specimens set the numerical aperture to
about 70%-80% of the objective’s N.A. If necessary, remove
on eyepiece and, looking into empty sleeve, adjust the con-
denser’s diaphragm in order to obtain an image like the one
in Fig. 11.
FFig. 11ig. 11
APERTURE
DIAPHRAGM
FIELD OF VIEW
30-20%
70-80%
FFig. 10ig. 10
①

Page 12
9. Maintenance
Microscopy environment
This microscope is recommended to be used in a clean, dry and shock free environment with a temperature of 5°- 40°C and
a maximum relative humidity of 85 % (non condensing). Use a dehumidier if needed.
To think about when and after using the microscope
• The microscope should always be kept vertically when moving it and be careful so that no moving
parts, such as the eyepieces, fall out.
• Never mishandle or impose unnecessary force on the microscope.
• Never attempt to service the microscope yourself.
• After use, turn off the light immediately, cover the microscope with the provided dust-cover, and keep
it in a dry and clean place.
Electrical safety precautions
• Before plugging in the power supply, make sure that the supplying voltage of your region matches
with the operation voltage of the equipment and that the lamp switch is in off- position.
• Users should observe all safety regulations of the region. The equipment has acquired the CE safety
label. However, users do have full responsibility to use this equipment safely.
Cleaning the optics
• If the optical parts need to be cleaned try rst to: use compressed air.
• If that is not sufcient: use a soft lint-free piece of cloth with water and a mild detergent.
• And as a nal option: use the piece of cloth moistened with a 3:7 mixture of ethanol and ether.
• Note: ethanol and ether are highly ammable liquids. Do not use them near a heat source, near sparks or near
electric equipment. Use these chemicals in a well ventilated room.
• Remember to never wipe the surface of any optical items with your hands. Fingerprints can damage the optics.
• Do not disassemble objectives or eyepieces in attempt to clean them.
For the best results, use the OPTIKA cleaning kit (see catalogue).
If you need to send the microscope to Optika for maintenance, please use the original packaging.

Page 13
10. Troubleshooting
Review the information in the table below to troubleshoot operating problems.
PROBLEM CAUSE SOLUTION
I. Optical Section:
LED operates, but eld of view remains
dark. Power supply is unplugged. Connect
Brightness is too low Set brightness to a proper level
Batteries discharged Charge the batteries
Dirt or dust is visible in the eld of view. Dirt/dust on the specimen Clean the specimen
Dirt/dust on the eyepieces Clean the eyepieces
Image looks double Aperture diaphragm is stopped down
too far Open aperture diaphragm
Visibility is poor.
• Image is not good.
• Contrast is poor.
• Details are indistinct.
• Image glares
Revolving nosepiece is in an incorrect
position Move the nosepiece to a click stop
Aperture diaphragm is too closed or to
open Adjust aperture diaphragm
Dust or dirt on lenses (condenser, ob-
jectives, eyepieces and slide) Clean thoroughly
For transmitted light observation, the
coverglass thickness must not exceed
0.17 mm
Use a coverglass with thickness 0.17
mm
Focus is not even Slide holder is not flat. Move the speci-
men to a flat position
One side of the image is out of focus. The nosepiece is not in the center of
the light path Turn the nosepiece to a click stop
The specimen is out of place (tilted) Place the specimen at on the stage.
The optical performance of the sample
cover glass is poor Use a cover glass of better quality
II. Electric Section:
The LED doesn’t turn on. No power supply Check the power cord connection
The brightness is not enough The brightness adjustment is low Adjust the brightness
The light blinks The power cord is poorly connected Check the power cord

Page 14
Equipment disposal
Art.13 Dlsg 25 July 2005 N°151. “According to directives 2002/95/EC, 2002/96/EC and 2003/108/EC relating to the reduc-
tion in the use of hazardous substances in electrical and electronic equipment and waste disposal.”
The basket symbol on equipment or on its box indicates that the product at the end of its useful life should be collected sepa-
rately from other waste. The separate collection of this equipment at the end of its lifetime is organized and managed by the
producer. The user will have to contact the manufacturer and follow the rules that he adopted for end-of-life equipment col-
lection. The collection of the equipment for recycling, treatment and environmentally compatible disposal, helps to prevent
possible adverse effects on the environment and health and promotes reuse and/or recycling of materials of the equipment.
Improper disposal of the product involves the application of administrative penalties as provided by the laws in force.

OPTIKA S.r.l.
Via Rigla, 30 - 24010 Ponteranica (BG) - ITALY Tel.: [phone]
[email protected] - www.optikamicroscopes.com
OPTIKA Spain
[email protected]
OPTIKA USA
[email protected]
OPTIKA China
[email protected]
OPTIKA India
[email protected]
OPTIKA Central America
[email protected]
®
®
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®
®

Ver. 2.1 2022
Modello
B-20R
B-20CR
M-100FX
M-100FLed
Serie ECOVISION
MANUALE DI ISTRUZIONI

Pagina 17
Sommario
1. Avvertenza 18
2. Informazioni sulla sicurezza 18
3. Disimballaggio 18
4. Utilizzo previsto 18
5. Simboli 18
6. Descrizione dello strumento 19
6.1 B-20R 19
6.2 B-20CR 20
6.3 M-100FX 21
6.4 M-100FLed 22
7. Assemblaggio 23
7.1 Procedura di assemblaggio 23
7.1.1 Montaggio delle batterie (M-100FLed) 23
7.1.2 Montaggio delle batterie (B-20R / B-20CR) 23
8. Uso del microscopio 24
8.1 Accensione del microscopio 24
8.1.1 B-20R / B-20CR / M-100FX 24
8.1.2 M-100FLed 24
8.2 Regolazione dell’intensità luminosa 24
8.2.1 M-100FX 24
8.2.2 B-20R / B-20CR / M-100FX 24
8.2.3 M-100FLed 24
8.3 Posizionare il campione 25
8.3.1 B-20R / M-100FX / M-100FLed 25
8.3.2 B-20CR 25
8.4 Uso con batterie 25
8.4.1 M-100FLed 25
8.4.2 B-20R / B-20CR 25
8.5 Diaframma di apertura 26
9. Manutenzione 27
10. Risoluzione dei problemi 28
Smaltimento 29

Pagina 18
1. Avvertenza
Questo microscopio è uno strumento scientico di alta precisione, progettato per durare a lungo con una minima
manutenzione; la realizzazione è secondo i migliori standard ottici e meccanici, per poter essere utilizzato quotidianamente.
Vi ricordiamo che questo manuale contiene informazioni importanti per la sicurezza e per la manutenzione dello strumento,
e deve quindi essere messo a disposizione di coloro che lo utilizzeranno.
Decliniamo ogni responsabilità derivante da un utilizzo dello strumento non indicato nel presente manuale.
2. Informazioni sulla sicurezza
Per evitare shock elettrici
Prima di collegare il cavo di alimentazione alla presa elettrica, assicurarsi che il voltaggio della rete locale coincida con il
voltaggio dello strumento e che l’interruttore dell’illuminazione sia nella posizione “OFF”.
Gli utenti dovranno seguire tutte le norme di sicurezza locali. Lo strumento è certicato CE. In ogni caso, gli utilizzatori sono
gli unici responsabili per un utilizzo sicuro dello strumento. Per l’utilizzo in sicurezza dello strumento è importante attenersi
alle seguenti istruzioni e leggere il manuale in tutte le sue parti.
3. Disimballaggio
Il microscopio è riposto in un imballo di polistirolo espanso. Rimuovere il nastro adesivo dal collo ed aprire la parte superiore
dell’imballo. Fare attenzione a non far cadere le parti ottiche (obiettivi e oculari) nell’estrarre il microscopio dalla scatola per
evitare che vengano danneggiati. Utilizzare entrambe le mani (una intorno allo stativo e una alla base), slare il microscopio
dal contenitore e appoggiarlo su un piano stabile.
Evitare di toccare le superci ottiche come lenti, ltri o vetri. Tracce di grasso o altri residui possono ridurre la
qualità visiva dell’immagine nale e corrodere la supercie delle ottiche in breve tempo.
4. Utilizzo previsto
Modelli standard
Solo per applicazioni di ricerca ed usi didattici. Non indicato per utilizzo diagnostico e terapeutico umano e veterinario.
Modelli IVD
Anche per uso diagnostico, nalizzato ad ottenere informazioni sulla situazione siologica o patologica del soggetto.
5. Simboli
La seguente tabella riporta i simboli utilizzati in questo manuale.
PERICOLO
Questo simbolo indica un rischio potenziale ed avverte di procedere con cautela.
SHOCK ELETTRICO
Questo simbolo indica un rischio di shock elettrico.

Pagina 19
OBIETTIVI
REVOLVER
TAVOLINO
OCULARE
MANOPOLA
MICROMETRICA
MANOPOLA
MACROMETRICA
STATIVO
ILLUMINATORE
VITE DI BLOCCO
TESTA DI OSSERVAZIONE
MANIGLIA DI
TRASPORTO
MOLLETTINE
CONDENSATORE
6. Descrizione dello strumento
6.1 B-20R

Pagina 20
MANOPOLE
TAVOLINO
OCULARE
MANOPOLA
MACROMETRICA
MANOPOLA
MICROMETRICA
DIAFRAMMA DI
APERTURA
ILLUMINATORE
VITE DI BLOCCO
6.2 B-20CR
TESTA DI OSSERVAZIONE
MANIGLIA DI
TRASPORTO
STATIVO OBIETTIVI
REVOLVER
TAVOLINO
MOLLETTINE
CONDENSATORE