Millipore Millicell ERS-2 User manual

SigmaAldrich.com
00108103w REV 0421 1 of 1000108103w REV 0421 1 of 10
User Guide
Millicell®ERS-2
Electrical Resistance System
MERS00002
Introduction
The Millicell
®
ERS-2 Electrical Resistance System is
a meter and electrode system designed to reliably
measure Trans Epithelial Electrical Resistance (TEER)
of epithelial cells in culture. An increase in TEER
detected with the electronic circuit of the Millicell
®
ERS-2 meter and its electrode is an indication of cell
monolayer health and confluence.
A silver/silver chloride (Ag/AgCl) pellet on each
electrode tip measures voltage. The small size of
the electrode enables the user to easily measure
transepithelial voltage and the resistance of cells
grown on microporous membranes.
This system is for research use only.
Benefits of AC Power Source
Over DC Power Source
The Millicell
®
ERS-2 system uses alternating current
(AC) to make membrane resistance measurements.
Using an isolated AC source has several advantages
over the traditional use of direct current (DC):
• Membrane voltage and voltage electrode offset
do not affect resistance measurements.
• The zero net charge on the cells eliminates the
adverse effects of DC current on the cell membrane.
• There is no electrochemical deposition
of electrode metals.
• Membrane capacitance does not affect resistance
readings. Once the Millicell
®
ERS-2 system is
standardized, you can use it to quantitatively
measure cell confluence.
The Millicell®ERS-2 System
and Components
The Millicell
®
ERS-2 system consists of a meter,
STX01 electrode, STX04 1,000 Ω test electrode,
and battery charger.
Analog output
Digital LCD display
Mode Switch
Input Port
R Adj Calibration
Power Switch
STX01 Electrode
STX04 Test
Electrode
The Meter
The unit measures 18.4 cm × 10.8 cm × 5.8 cm
and includes an internal rechargeable 6 V NiMH
2,700 mAh battery pack with an external 12 V DC
battery charger, equipped with an AC power cord.
An analog output port allows output of analog data
to a recording device.
The Electrode
The electrode is connected to a wire with
a telephone-type plug at its end. This plug connects
to the Input port at the front of the meter during use.

00108103w REV 0421 2 of 10
Symbols Used in this User Guide
The following symbols are used throughout this user
guide and/or on product labels, and the user shall
abide by indicated requirements:
Symbol Definition
Y
Warning alerts you to actions that may
cause personal injury or pose a physical
i
Read the documentation
h
Catalogue number
f
Serial number
M
Manufacturer
C
CE conformity marking. Reference
Declaration of Conformity for specific
Do not discard with common solid
waste at end of life. Segregate
with other waste electrical and
electronic equipment (WEEE) and
send to an appropriate facility for
recycling. For information on recycling
electrical and electronic products
in the European Union, please visit
Installation
At least 24 hours before intended use, unpack the
system, install the supplied power cord, and plug cord
into AC wall outlet. Charge the battery overnight.
DO NOT try to use the system while the battery
is charging. When not in use, the meter should be
powered off and disconnected from the charger, as
continuous charging may shorten battery life.
NOTE: The Millicell
®
ERS-2 meter is designed to
operate on battery power only, and should hold a
charge for 8–10 hours. ALWAYS disconnect the meter
from the AC wall charger before using it. Leaving
the meter plugged in during use causes undesirable
electrical noise, and may result in unstable readings.
Testing the System
The Millicell
®
ERS-2 meter and electrode should be
tested periodically to ensure proper operation. Test
the system when you first receive it, at the beginning
of a series of measurements, and as needed during
the course of the measurements to confirm stability.
Prior to each use, the following functionality
checks should be performed:
• Meter functionality check (for resistance
and voltage measurements)
• Electrode functionality check (for voltage
measurements only)
Meter Functionality Check
1. Disconnect the Millicell
®
ERS-2 meter from the
charger.
2. Connect the STX04 test electrode (electrode with
the short wire) to the meter by inserting the plug
at the end of the flexible electrode cable into the
INPUT port on the meter.
3. Turn the POWER switch On and set the MODE switch
to Ohms (Ω).
4. The meter should display 1000 Ω. If not, adjust
the R Adj screw with a small flathead screwdriver
(not included) until the meter shows a reading of
1000 Ω.
I
000

00108103w REV 0421 3 of 10
Equilibrating the Electrode
for Voltage Measurements
When the Millicell
®
ERS-2 system is used for
resistance measurements only, the electrode can
be used directly out of dry storage without any
equilibration. The unit’s design compensates for any
asymmetry of measurement.
For voltage measurement, the STX01 electrode needs
to be equilibrated to eliminate any offset before use.
To equilibrate, prepare an electrolyte solution similar
to the one you will use when taking measurements,
or use phosphate buffered saline (PBS), 0.15 M
sodium chloride (NaCl), or 0.1 M potassium chloride
(KCl).
1. Disconnect the Millicell
®
ERS-2 meter from the
charger.
2. Connect the STX01 electrode to the meter by
inserting the plug at the end of the flexible
electrode cable into the INPUT port on the meter.
3. With the POWER switch Off, and the MODE switch
in the Ohms position, immerse the electrode in the
electrolyte solution. When the voltage electrode
pairs are connected to the instrument with the
instrument power off, they are shorted together
internally, and this allows the probes to equilibrate.
During this equilibration, the asymmetrical
potential difference across the two voltage
electrodes is reduced and the inter-electrode DC
potential will be a few millivolts or less and quite
stable. The following table lists the recommended
equilibration time.
If the electrode has... then soak it in solution for...
never been tested
for voltage drift 24 hours
been stored dry 24 hours
been stored in
solution
2 hours
4. Perform the “Electrode Functionality Check”
(see below).
Electrode Functionality Check
(for voltage measurements only)
1. Set the MODE switch to Millivolts and disconnect
the meter from the charger.
2. Insert the equilibrated electrode into a cell culture
insert containing culture media or electrolyte
solution.
3. Turn the power on.
4. The meter should display a steady voltage reading
of the trans-membrane potential, indicating that
the electrode is ready for voltage measurements.
5. If the reading of the culture media is unstable or
greater than 20 mV, equilibrate the electrode again
for 12–24 hours while attached to the meter. The
POWER switch should be Off and the MODE switch
on Ohms.
6. Repeat steps 1–4. If the reading remains unstable,
or greater than 20 mV, refer to “Troubleshooting”.
Measuring Cell Resistance and Voltage
Set up the laminar flow hood with the following:
• Fully charged Millicell
®
ERS-2 system
• STX04 test electrode
• STX01 or STX03 electrode (for Millicell
®
6, 12,
and 24-well inserts/plates), or STX00 electrode
(required for Millicell
®
-96 cell culture plates),
equilibrated if being used to measure voltage
• Millicell
®
culture plate insert without cells (control)
• Millicell
®
culture plate insert with cells
• 70% ethanol solution
• Small flathead screwdriver
NOTE: When the system is not in use, turn off the
POWER switch. Use aseptic technique to maintain
culture sterility.
Sterilizing or Sanitizing/Disinfecting
the Electrode
The Millicell
®
ERS-2 electrode is supplied non-sterile.
To sterilize, use standard sterilizing gas mixtures. To
sanitize/disinfect, use a low temperature chemical
sanitizing/disinfecting solution such as ethanol,
isopropanol, or 5% sodium hypochlorite.
Sterilize or sanitize/disinfect the electrode immediately
before use if the cultures will be returned to the
incubator.
CAUTION: DO NOT autoclave or flame the electrode;
it cannot withstand high temperatures. DO NOT
expose the electrode to ultraviolet light; UV light
decomposes silver/silver chloride electrodes.
To sanitize/disinfect using alcohol or 5% sodium
hypochlorite, follow this procedure in a laminar flow
hood.
1. Immerse the electrode tips in sanitizing/disinfecting
solution for 15 minutes. Allow to air dry for
15 seconds.
NOTE: Do not leave the electrode tips in alcohol
for more than 30 minutes at a time. Continuous
soaking will weaken the electrode’s protective
coating and shorten its life.
2. Rinse the electrode in a sterile electrolyte
solution similar to the cell culture media
or in 0.1–0.15 M KCl or NaCl.
3. For resistance measurements, the electrode
is now ready to use.
4. For voltage measurements, allow the electrode to
equilibrate in the sterile electrolyte solution similar
to the cell culture media for 15 minutes.

00108103w REV 0421 4 of 10
Measuring Cell Resistance
The preferred application for the Millicell
®
ERS-2
system is measuring electrical resistance in cells.
To measure resistance, follow these steps.
NOTE: Although the meter can read up to 13,000 Ω,
accuracy is only guaranteed up to 9,999 Ω.
1. Allow cells to come to room temperature.
2. Test the Millicell
®
ERS-2 system as described
on page 4.
3. Make sure that the meter is disconnected from
the charger.
4. Set the MODE switch to Ohms and turn the POWER
switch On.
5. Immerse the electrode so that the shorter tip is in
the Millicell
®
culture plate insert and the longer tip is
in the outer well. The shorter tip should not contact
cells growing on the membrane and the longer tip
should just touch the bottom of the outer well. To
ensure stable and reproducible results, make sure
that the electrode is held steady and at a 90° angle
to the plate insert.
NOTE: If rinsing is required between measurements
to prevent sample carryover, use culture media
rather than distilled water.
II
0 0
6. Record the resistance.
How to Determine Blank Resistance
1. Add electrolyte to a blank cup
(i.e., the cell culture insert without cells).
2. Measure the resistance across the blank cup
as in step 5 of the previous section.
Resistance Calculations
Tissue Resistance
To obtain true tissue resistance, subtract the
resistance reading across the blank cup from the
resistance reading across the tissue (cell culture
insert with cells).
Unit Area Resistance
The resistance is inversely proportional to the area
of the tissue. The larger the membrane, the lower
the resistance.
NOTE: Resistance readings for 24 mm or larger
diameter inserts should not be converted to unit
area resistance because the STX electrode cannot
deliver a uniform current density over the relatively
large area of membrane. This is not a problem with
smaller inserts because of the relatively smaller area
of membrane compared to the electrode. See table on
next page for diameters of Millicell
®
cell culture inserts
and plates.
To achieve consistency across different plate formats
(excluding 24 mm or larger diameter inserts), the
product of the resistance and the area is typically
calculated and reported. This value is independent
of the area of the membrane used.
The unit area resistance is obtained by multiplying the
meter reading by the effective surface area of the filter
membrane. The unit of measure is Ωcm
2
. Effective
membrane areas for Millicell
®
cell culture inserts and
plates are listed in the table below.
Unit Area
Resistance
= Resistance (Ω) × Effective Membrane Area(cm
2
)
Unit Area = 1 cm
2
The unit area resistance is independent of the area of
the membrane used and may be used to compare data
obtained from 12 mm or smaller inserts.
Millicell®Cell Culture Insert and Plate Effective
Membrane Area
Product
Well Diameter
(mm)
Membrane
Area (cm
2
)
6-well standing
insert
30 4.2
24-well standing
insert
12 0.6
6-well hanging insert 24 4.5
12-well hanging
insert
12 1.1
24-well hanging
insert
6.5 0.3
24-well plate ~ 10 0.7
96-well plate ~ 4 0.11
Example Application
The following is an example of a typical Millicell
®
ERS-2 application. It is not a technically complete
experimental protocol.
Culture Cells
Seed cells onto 22 of 24 properly prepared, 24-well
Millicell
®
-CM culture plate inserts, leaving the other
two Millicell
®
culture plate inserts blank. Grow cells to
confluency, according to standard protocol.
Measure and Calculate Resistance
1. Sterilize or sanitize/disinfect the electrode according
to instructions on pages 6–7.
2. Allow the samples to come to room temperature.
3. Measure the resistance of the two blanks
(i.e., Millicell
®
-CM inserts without cells).
4. Measure cell resistance in the 22 sample wells
(i.e., Millicell
®
-CM inserts with cell monolayers).
5. Repeat step 3.

00108103w REV 0421 5 of 10
6. Average the resistance measurements of the two
blanks and arrive at a value.
For example: R
blank
= 180 Ω
7. Subtract this blank resistance from the sample-well
resistance measurement. Suppose a typical sample
reading is 320 Ω.
Then: R
sample
– R
blank
= R
cell monolayer
320 Ω – 180 Ω = 140 Ω
The resistance of the cell monolayer in this example
is 140 Ω. However, you still need to correct for the
area covered by the cell monolayer.
8. To correct for the area covered by the cell
monolayer, calculate the product of the resistance
and the area. Multiply the area of effective
membrane diameter on the Millicell
®
culture plate
insert by the resistance found in the experiments:
9. 140 Ω × 0.6 cm
2
= 84 Ω cm
2
NOTE: The 24-well Millicell
®
-CM insert has an
effective membrane area of 0.6 cm
2
.
This value, 84 Ω cm
2
, is independent of the area of
membrane used.
Measuring Voltage
Follow these steps to measure voltage.
1. Allow the cells to come to room temperature.
2. Test the Millicell
®
ERS-2 system as described on
page 4. Make sure that the electrode has been
equilibrated and tested according to the procedures
on pages 5–6.
3. Disconnect the meter from the charger. Set the
MODE switch to Millivolts and turn the POWER
switch On.
4. Immerse the electrode so that the shorter tip is in
the Millicell
®
culture plate insert and the longer tip is
in the outer well. The shorter tip should not contact
cells growing on the membrane, but the longer tip
should just touch the bottom of the outer well. To
ensure stable and reproducible results, make sure
that the electrode is held steady and at a 90° angle
to the plate insert.
I.I
5. Record the voltage. Subtract the voltage of the
culture media alone (step 4, Electrode Functionality
Check) to obtain the true voltage value of the
cultured cell monolayer.
NOTE: If rinsing is required between measurements
to prevent sample carryover, use culture media
rather than distilled water.
In voltage mode, if the meter reading is positive, it
means that the basal side of the cellular tissue (the
side adhering to the culture insert filter) is positive
relative to the apical side (exposed). Conversely, if the
meter is reading negative, it means that the basal side
is negative relative to the apical side.
Maintaining and Storing the System
Meter
Charge the batteries monthly or when BATT appears
in the digital display.When not in use, the meter should
be powered off and disconnected from the charger, as
continuous charging may shorten battery life.
NOTE: Always power the meter off when charging the
battery.
The meter should hold a charge for approximately 8
hours. It will shut off automatically when the battery
runs low to avoid damage to the instrument. When
the battery charge is low, BATT appears in the digital
display. To avoid automatic shut-off, recharge the
battery when the BATT warning is displayed.
CAUTION: To prevent corrosion, do not splash or
spill saline solution or culture media on the meter. To
prevent the digital LCD display from darkening, do not
leave the meter in direct sunlight for long periods of
time.
Changing the Battery
The Millicell
®
ERS-2 meter is equipped with a 6 V 2,700
mAh rechargeable NiMH battery pack which can be
charged for approximately 500 cycles. The battery
pack may be charged at any time in the discharge
cycle and can be charged continuously without
damage, using the supplied charger.
CAUTION: To prevent battery damage, use ONLY the
supplied charger.
The battery life is about 1 to 2 years, depending on
use. NiMH batteries discharge even if they are not in
use. To prevent battery damage from self discharge,
charge the battery pack monthly.
To change the battery:
1. Using a #1 Phillips screwdriver, remove the four
screws from the Millicell
®
ERS-2 case and separate
instrument cover from base.
2. The battery pack is mounted to the base by a metal
cover.
Remove the two screws and lift battery cover and
battery out.
3. Remove battery pack from cover.
4. Note the position of the battery pack connector and
wire color code, so that the correct polarity can be
maintained with the new battery pack. Disconnect
the small plastic connector from the circuit board by
pulling it straight out from the circuit board.
CAUTION: Connecting the battery connector with
the polarity reversed may result in personal or
equipment damage.

00108103w REV 0421 6 of 10
5. Place new battery pack in battery cover and
resecure to base.
6. To reconnect battery pack wire to circuit board,
line up the connector of the battery pack so that
the protruding ledge faces the tab (locking guide)
on the circuit board. Push the connector straight
down over the pins on the board. Do not force the
connector; if aligned properly, it should click into
place with little effort.
7. Reassemble the instrument cover and base.
8. Charge the new battery for at least 12 hours.
9. Dispose of old battery in accordance with local
regulations.
Electrode Storage
• Short-term storage (overnight): Immerse
the electrode tip in electrolyte solution. Keep the
electrode cable plugged into the electrode port on
the meter so that the system is internally short-
circuited and electrode symmetry (equilibration)
is maintained.
• Long-term storage: Rinse the electrode with
Milli-Q
®
water or equivalent and store dry and
in the dark. Do not allow electrode to dry without
first rinsing with Milli-Q
®
water to remove salts
and proteins.
Electrode Cleaning
With repeated use, the electrode surface can become
coated with protein or other foreign materials. This
build-up can degrade the performance of the system.
After every use, rinse the electrodes with Milli-Q
®
water and store them as indicated above. Periodically
clean the electrodes with Tergazyme
®
detergent
(Alconox, Inc.
®
cat. no. 1304) as follows:
1. Rinse the electrodes with Milli-Q
®
water
and dry them.
2. Make a 1% solution of Tergazyme
®
detergent
and suspend the electrode tips in the solution
with the exposed electrode surfaces fully immersed.
If desired, brush the electrode surfaces with a
soft brush (e.g., tooth brush).
If the electrode is...
Then soak it in solution for...
not cleaned routinely 12 hours
is on a weekly
cleaning schedule
30–60 minutes
is cleaned daily 5 minutes
3. Rinse well with Milli-Q
®
water. Store as detailed in
Electrode Storage section.
Sanding (only when measuring voltage)
4. Lightly rub the voltage electrodes (the two silver
pellets on the inner surfaces near the electrode tips)
with 600-grade ultrafine sandpaper (provided) or
an ink eraser. Do not sand the silver pellets on the
outside of the electrode tips.
CAUTION: Only a very thin surface layer of the
pellet should be removed. Repeated rubbing will
eventually remove the Ag/AgCl pellet. When rubbing
no longer improves the voltage readings, the
electrode should be replaced.
Troubleshooting
Most of the time, system problems are related to the
electrode rather than the meter itself. However, if the
meter does fail, it is most often due to switch failure
caused by corrosion from accidental spillage of saline
solution or culture media on the meter. If functional
testing indicates acceptable performance, and the
meter has not been exposed to salt solutions, then
the meter is working correctly.
When the electrode fails, the most common symptom
is an unstable or unusually high reading. The following
qualitative method may be helpful in determining
whether or not the electrode is functional:
• Check the resistance of a well filled with electrolyte
and across a blank culture insert filled with
electrolyte. The resistance of electrolyte alone
should be less than 50 Ω and stable if the electrode
is kept stationary. The resistance of the blank insert
is normally in the 80 to 200 Ω range, depending
on the brand and size.
• When the meter displays a lower than expected
resistance, but is stable and reproducible, the most
likely cause is the cell culture, not the electrode
or the meter.
• During normal use, it may helpful to write down
the resistance range of each particular type of blank
insert with the specific culture media used. If the
electrode is subsequently suspected of having a
problem, a comparison of current readings with past
readings on the same blank insert and culture media
may assist in determining if the electrode
is functioning properly.

00108103w REV 0421 7 of 10
Symptom Cause Corrective Action
Meter will not power on when
disconnected from charger
OR
Meter runs briey,
then powers o
Batteries are discharged
or insuciently charged
The meter will power down automatically when the batteries are depleted.
Power o the meter, connect it to the battery charger, and charge for at
least 12 hours. A full charge may require up to 24 hours. For reliability, the
meter should be charged each evening (with the power o) before use the
next day.
Batteries are defective Replace the battery pack or contact Technical Service.
Meter will not charge
when connected to charger
Insecure connection at power
input jack on meter
Verify that the battery charger is securely connected at the meter power
input jack.
Defective charger Using a volt meter, verify correct output voltage at the charger connector.
If the volt meter reading is not close to +12 V DC at the center conductor
on the connector, replace the battery charger. If the battery charger is
working and the unit does not power up, contact Technical Service.
Meter reading is unstable
when power is turned on
Meter is connected
to charger
Disconnect the meter from the charger.
Low battery charge Recharge battery for 12 hours, then perform “Meter Functionality Check”.
Failure to achieve specied
test values during “Meter
Functionality Check”
Low battery charge Recharge battery for 12 hours and repeat “Meter Functionality Check”.
If readings are still not as specied, contact Technical Service.
Intermittent meter reading Loose or broken electrode
connector or cable
Replace the electrode.
Malfunctioning system Call Technical Service.
Unstable resistance
or voltage reading,
(voltage drift) during use
Meter is connected
to charger
Disconnect the meter from the charger.
Electrode not immersed
in culture media solution
Make sure both tips are immersed in solution.
NOTE: When the meter is in voltage mode, it is normal for the display
to be unstable when the electrode is not immersed in solution.
Electrode position
not stable
Hold electrode in a xed position at a 90° angle to the plate insert.
Hand held electrodes must be kept as motionless as possible during a
measurement.
VOLTAGE ONLY:
Electrode not equilibrated
Condition the electrode as outlined in “Equilibrating the Electrode”.
Dirty electrode tips Clean the electrode as outlined in the “Electrode Cleaning” section.
Electrode is old or broken Replace the electrode. If “Meter Functionality Check” indicates that meter
is functional, electrode may be broken or have exceeded its normal lifetime
of 1 to 2 years.
RESISTANCE ONLY:
Meter is malfunctioning
Verify a reading of 1,000 Ω with the test electrode. Adjust if required.
If adjustment cannot be made, call Technical Service.
Electrode and cable too close
to strong electromagnetic
radiation
Move the system to a dierent area.
Meter connected to a
recording device via the
output jack
Remove the connection to the recording device.
Slow response in resistance
or voltage reading
Measurement conditions need
to be optimized
Make sure electrode is fully wetted and the solution is at room temperature.
Make sure there are no bubbles under the Millicell®culture plate insert.
Take measurements with electrode tips completely immersed in solution.
VOLTAGE ONLY:
Electrode not equilibrated
Condition the electrode as outlined in “Equilibrating the Electrode”.
Dirty electrode tips Clean electrode as outlined in “Electrode Cleaning”. After use, make sure
electrode is rinsed with Milli-Q®water before it dries.
Resistance reading is lower
than expected
Meter is malfunctioning Perform “Meter Functionality Check”.
Cell culture contaminated Re-culture cells.
Cells have not
formed tight junctions, or are
not conuent
Allow cells to grow longer.
Resistance reading is higher
than expected
Meter is malfunctioning Perform “Meter Functionality Check”.
Electrode contacts have a
buildup of protein or other
foreign material
Clean the electrode as outlined in “Electrode Cleaning”.
Electrode broken Replace the electrode.

00108103w REV 0421 8 of 10
Symptom Cause Corrective Action
Voltage reading higher than
20 mV during “Electrode
Functionality Check”
Meter is connected
to charger
Disconnect the meter from the charger.
Low battery charge Recharge battery for 12 hours.
Electrode not equilibrated Condition the electrode as outlined in “Equilibrating the Electrode”.
Dirty electrode tips Clean the electrode as outlined in “Electrode Cleaning”.
Conductive contamination
between electrodes
Inspect the inter-electrode surface areas for material which could form
a conductive bridge between the inner and outer electrodes. If the
material cannot be removed, replace the electrode.
Corrosion on electrode
conductive traces
Inspect the embedded copper circuit traces that run from the electrode
tip and up the handle. If any black discoloration is observed at the tip,
replace the electrode.
Specifications
Millicell®ERS-2 Meter
Membrane voltage range ± 200.0 mV
Voltage measurement 0.1 mV
Resistance range 0 to 9,999 Ω
Resistance resolution 1 Ω
AC square-wave current ± 10 µA nominal at 12.5 Hz
Power Internal 6 V NiMH 2,700 mAh battery
with external 12 V DC supply for recharging
Nominal battery run time ~ 8 hours*
Analog output Millivolt mode: 1 mV = 10 mV
Ohm mode: 1 Ω = 1 mV
Environmental range 50–100 °F (10–38 °C)
0–90% non-condensing relative humidity
Dimensions 7.25 in. (18.4 cm) × 4.25 in. (10.8 cm) × 2.3 in. (5.8 cm)
Weight 3 lb (1.4 kg)
* When the battery power level falls below a minimum threshold, the meter automatically powers off.
A fully charged battery will provide about 8 hours of running time.
Regulatory Compliance
This product is in conformity with the following standards, and therefore meets the provisions
of European LV Directive 2006/95/EC and European EMC Directive 2004/108/EC.
Safety: EN 61010-1: 2001 (Eqv: IEC 61010-1:2001)
EMC: EN 61326-1: 2006 (Eqv: IEC 61326-1:2005)
EN 61326-2-3: 2006 (Eqv: IEC 61326-2-
3:2006)

00108103w REV 0421 9 of 10
Ordering Information
Purchase products online at www.sigmaaldrich.com/products or see contact information.
Millicell®ERS-2 System and Components
Product Description Qty/Pk Cat. No.
Millicell
®
ERS-2 system
(includes meter, battery, STX01 electrode, 1,000 Ω test electrode, battery charger) 1 MERS00002
Replacement electrode 1 pair MERSSTX01
Adjustable electrode 1 pair MERSSTX03
Electrode for Millicell
®
-96 well plate 1 pair MERSSTX00
Replacement 1,000 Ω test electrode 1 MERSSTX04
Replacement Battery 1 MERSBAT01
Other Millicell®Products
Millicell®Single-well Standing Inserts
Membrane
Pore
Size
Device
Size Qty/Pk Cat. No.
Organotypic insert**
Biopore™ (Polytetrafluoroethylene, PTFE) 0.4 µm 6-well 50 PICM0RG50
HA insert
MF-Millipore™
(Mixed cellulose esters)
0.45 µm 24-well
6-well
50
50
PIHA01250
PIHA03050
CM insert**
Biopore™ (PTFE) 0.4 µm 24-well
6-well
50
50
PICM01250
PICM03050
PCF insert
Isopore™
(Polycarbonate, PCF)
0.4 µm
3 µm
8 µm
12 µm
0.4 µm
24-well
24-well
24-well
24-well
6-well
50
50
50
50
50
PIHP01250
PITP01250
PI8P01250
PIXP01250
PIHP03050
** For adherent cells, this membrane needs to be coated with a extracellular matrix.
Millicell®Single-well Hanging Inserts
Membrane
Pore
Size
Device
Size Qty/Pk Cat. No.
PET Insert
(Polyethylene terephthalate, PET)
0.4 µm
1 µm
3 µm
5 µm
8 µm
6-well 48 MCHT06H48
MCRP06H48
MCSP06H48
MCMP06H48
MCEP06H48
0.4 µm
1 µm
3 µm
5 µm
8 µm
12-well 48 MCHT12H48
MCRP12H48
MCSP12H48
MCMP12H48
MCEP12H48
0.4 µm
1 µm
3 µm
5 µm
8 µm
24-well 48 MCHT24H48
MCRP24H48
MCSP24H48
MCMP24H48
MCEP24H488

00108103w REV 0421 10 of 10
The life science business of Merck operates
as MilliporeSigma in the U.S. and Canada.
The initial M, MilliporeSigma, Millipore, Biopore, Isopore, MF-Millipore, Millex, Millicell, Milli-Q,
Stericup, Steriflip, and Sigma-Aldrich are trademarks of Merck KGaA, Darmstadt, Germany
or its affiliates. All other trademarks are the property of their respective owners. Detailed
information on trademarks is available via publicly accessible resources.
© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.
Millicell®-24 Well Cell Culture Insert Plate Assemblies
System
Components
Membrane
(Pore size) Qty/Pk Cat. No.
24-well cell culture plate, single-well feeder tray, 24-
well receiver plate, lid
PCF (0.4 µm)
PET (1 µm)
PCF (3 µm)
PCF (5 µm)
PCF (8 µm)
1 PSHT010R1
PSRP010R1
PSST010R1
PSMT010R1
PSET010R1
24-well cell culture plate, 24-well
receiver plate, lid
PCF (3 µm)
PCF (5 µm)
PCF (8 µm)
5 PSST010R5
PSMT010R5
PSET010R5
24-well cell culture plate, single-well feeder tray, lid PCF (0.4 µm)
PET (1 µm)
5 PSHT010R5
PSRP010R5
24-well receiver plate, lid N/A 5 PSMW010R5
Single-well feeder tray, lid N/A 5 PSSW010R5
Millicell®-96 Well Cell Culture Insert Plate Assemblies
System
Components
Membrane
(Pore size) Qty/Pk Cat. No.
96-well cell culture plate, single well feeder tray,
96-well receiver plate, lid
PCF (0.4 µm)
PET (1 µm)
1 PSHT004R1
PSRP004R1
96-well cell culture plate, 96-well receiver plate, lid PCF (0.4 µm) 5 PSHT004S5
96-well cell culture plate, single-well feeder tray, lid PCF (0.4 µm)
PET (1 µm)
5 PSHT004R5
PSRP004R5
96-well receiver plate, lid N/A 5 MACAC0RS5
Accessories
Description Qty/Pk Cat. No.
Stericup
®
-GP filter unit, 150 mL, PES membrane 12 SCGPU01RE
Sterile Millex
®
-GP 33 mm filter unit, PES membrane 50 SLGP033RS
Steriflip
®
-GP filter unit, PES membrane 25 SCGP00525
Notice
We provide information and advice to our customers
on application technologies and regulatory matters
to the best of our knowledge and ability, but without
obligation or liability. Existing laws and regulations
are to be observed in all cases by our customers.
This also applies in respect to any rights of third
parties. Our information and advice do not relieve
our customers of their own responsibility for
checking the suitability of our products for the
envisaged purpose.
The information in this document is subject to change
without notice and should not be construed as a
commitment by the manufacturing or selling entity,
or an affiliate. We assume no responsibility for any
errors that may appear in this document.
Technical Assistance
Visit the tech service page on our website
at SigmaAldrich.com/techservice.
Contact Information
For the location of the office nearest you,
go to SigmaAldrich.com/offices.
Standard Warranty
The applicable warranty for the products listed
in this publication may be found at
SigmaAldrich.com/terms.
NOTE: The warranty for electrodes and batteries
is 30 days from date of receipt.
M
EMD Millipore Corporation
Burlington, MA U.S.A.,
an affiliate of Merck KGaA,
Darmstadt, Germany. C
For research use only. Not for use in
diagnostic procedures.