Millipore XX4004700 User manual

Introduction
The 47 mm Pressure Filter Holder is used to clarify or sterilize small batch volumes
of liquid. The cylindrical barrel holds 100 or 340 mL of liquid, depending on the
model. The liquid is filtered through a 47 mm disc filter of appropriate pore size
by applying external gas pressure via a standard gas cylinder or oil-free vacuum/
pressure pump (see Product Ordering Information section for available pumps). An
optional depth-type prefilter placed upstream of the membrane filter will prevent
premature clogging when processing viscous or particulate-laden liquids. The filter
holder can be autoclaved with the filter installed.
Filter Holder Components
Top cap with hose adapter
Top cap O-ring
Barrel (100 or 340 mL capacity)
Locking ring
Locking ring gasket
Depth prefilter (optional)
Filter sealing 0-ring
Filter
Filter support screen
Base
Stopper
Wrenches for tightening
locking ring not shown
Disassembly and Cleaning
Clean the filter holder before using for the first time and after every use.
1. Use the two wrenches supplied if necessary to unscrew the locking ring and
release the base. Unscrew the top cap from the upper end of the barrel and slide
the locking ring and locking ring gasket off the barrel. Use smooth-tip forceps
to remove the top cap O-ring and filter sealing O-ring. To remove the filter
sealing O-ring, gently pry it out of the base by inserting the forceps into the
small rounded “O-ring pick-out” recess. Tip the filter support screen out of the
base onto a clean surface.
NOTE: If the filter support screen does not readily drop out of its recess, screw
the locking ring part way onto the base, then invert the assembly and
tap it against the bench top to dislodge the screen.
2. Clean all components thoroughly with a sponge, hot water, and a nonabrasive
cleanser. Clean threaded parts, recesses, and orifices with a stiff bristle brush
and use a pipe cleaner to remove contaminants from inlet and outlet orifices.
CAUTION: Never use steel wool or abrasive materials on any part of the filter
holder. Take particular care not to scratch the polytetrafluoro-
ethylene (PTFE) sealing surface.
3. Rinse all parts thoroughly with hot running water, then rinse several times with
cold laboratory-grade water. Rinse new pressure tubing with hot tap water prior
to initial use.
4. Allow the components to air dry while disassembled.
CAUTION: Do not wipe dry with paper or cloth, as this leaves fibers and lint on
the surfaces and also generates electrostatic forces that can attract
more dirt. Filtered compressed air can be used to facilitate drying.
Assembly
1. Seat the top cap O-ring in the recess in the cap and the filter O-ring in the
O-ring groove in the barrel.
2. Slip the locking ring gasket over the threaded end of the barrel, followed by the
locking ring. Support the barrel vertically in a ring stand clamp. Position the
clamp high enough to place a filtering flask or beaker under the outlet tube, and
tighten the clamp enough to prevent the barrel from turning.
3. Seat the filter support screen evenly in the holder base, screen-side up. Holding
the base upright, remove a 47 mm
diameter filter from its package
with smooth-tip forceps, and center
the filter evenly on the holder base.
If using a depth-type prefilter disc
(42 mm diameter), center it exactly
on top of the membrane filter. It
must be positioned to lie within the
sealing O-ring at the bottom of the
barrel once the base is secured to
the barrel.
4. Lift the locking ring to expose the sealing O-ring in the end of the barrel. Raise
the base and filter until it
makes contact with the
O-ring around the perimeter
of the barrel. Then lower the
locking ring so that it
contacts the base. Hold the
base steady and turn the
locking ring clockwise until
you feel resistance.
5. Grasp the base with the small
wrench (it fits into the slots
on the base) and tighten the locking ring with the
larger wrench. Apply enough force to seal the filter effectively.
CAUTION: Avoid moving the base, as this may damage the membrane.
Autoclaving
When autoclaving with the filter in place, it is important that the steam be able
to access both sides of the filter at the same time, so that there is no differential
pressure across the filter, especially while it is hot. To ensure that there are no flow
restrictions on the filter holder inlet and outlet, make sure that tubing (if attached)
is not kinked. If tubing ends are wrapped, use wrapping material that breathes freely.
User Guide
Stainless Steel 47 mm Pressure Filter Holder
Cat. Nos. XX4004700 (100 mL) and XX4004740 (340 mL)
Disassembly and Cleaning, continued
M12194_Rev1215.indd 1 12/22/2015 8:41:31 AM

The M logo, Millipore, and Durapore are registered trademarks of Merck KGaA, Darmstadt, Germany.
Fluoropore and MF-Millipore are trademarks of Merck KGaA.
© 2015 EMD Millipore Corporation. Billerica, MA, U.S.A. All rights reserved.
M12194, Rev. 12/15
Make sure that any tubing used is autoclavable and can withstand the maximum
filtering pressure.
Acceptable tubing: pure gum rubber, silicone rubber, PTFE-lined tubing
Unacceptable tubing: surgical latex, black rubber compounds
1. Make sure that all inner surfaces of the filter holder are completely dry, then
assemble the holder with a filter in place. Hand tighten the locking ring.
2. Screw on the top cap, but do not fully tighten.
3. Wrap the exposed ends of the filter holder with lint-free autoclave paper
and secure with autoclavable tape or a rubber band. If desired, a length of
autoclavable tubing not exceeding 1.5 meters (5 feet) can be attached to the
outlet tube.
4. Autoclave the filter holder at 121 °C 1 bar (15 psi) for 25minutes. Allow the
filter holder to cool, then tighten the locking ring using the wrenches provided.
Checking System Integrity (Optional)
A wet membrane filter will not allow gas to pass through the pores at any pressure
below a critical value. You can detect a damaged filter or assembly leak with the
following test:
1. Attach a short length of flexible tubing to the holder outlet, and place the end
of the tubing in a glass beaker or other open receptacle. Place enough water in
the beaker to cover the open end of the tubing.
2. Pour a small amount of the liquid you want to filter into the filter holder barrel.
Screw down the top cap, connect pressure tubing, and apply low pressure
~ 0.2bar (~ 3 psi). All liquid above the filter will pass through to the beaker, and
flow should then stop. If no bubbles are seen, skip to step 4. Bubbles or a rapid
continuous flow of gas exiting the outlet tubing indicate inadequate wetting,
filter failure, or incomplete sealing.
3. If bubbles are seen, first try rewetting the filter (step 2). If the same thing
happens, disassemble the system, inspect membrane for damage, and ensure
that there is no dirt, debris, or damage to the O-ring or sealing surface. Install a
new filter and repeat loading, autoclaving, and system integrity steps 1 and 2.
4. Slowly increase pressure, in 0.3 bar (5 psi) increments to approximately 80% of
the bubble point pressure for the installed filter. Typical bubble points can be
found at www.millipore.com. Enter the family name (Durapore, MF-Millipore,
Fluoropore, etc. + membrane filters) in the search box. Continue to increase
pressure in 0.3 bar (5 psi) increments, pausing 10–15 seconds after each
increase. The test is successful if you reach or exceed the typical bubble point.
NOTE: If the holder contains multiple filters (as in a serial filtration), or a
prefilter in addition to the membrane filter, gas trapped against the wet
membrane after a bubble point test can produce an air-lock that will
restrict flow. You can overcome this by raising the filtering pressure over
the bubble point of the smallest pore size filter used.
Filtering the Sample
NOTE: If the system has been autoclaved, use aseptic technique.
1. Place the assembled filter holder on a ring stand and place a suitable receiving
vessel under the filter holder outlet to collect the filtrate.
CAUTION: When using the silicone stopper attached to the base to seat the
filter holder in a flask receiving vessel, air must be allowed escape
from the flask during filtration.
2. Remove the top cap, if attached, and pour the sample into the holder barrel,
Screw the top cap down tightly. Attach pressure tubing to the top cap tubing
adapter using a hose clamp, and apply pressure from an inert gas or filtered air
supply to filter the sample. Do not exceed the maximum recommended pressure
of 6.9 bar (100 psi).
3. When filtration is complete, relieve internal pressure in the holder with the
regulator valve on the pressure source. If the regulator valve does not allow for
such venting, do not attempt to remove the pressure hose connection. Instead,
slowly unscrew the top cap until you hear the sound of gas escaping. When the
sound ceases, remove the pressure tubing and open the holder.
Specifications
Maximum pressure 6.9 bar (100 psi) inlet and differential
Filter diameter 47 mm
Prefilter diameter 42 mm (thick depth prefilter)
Effective filter area 14.3 cm2(2.2 in2)
Capacity 100 mL (cat. no. XX4004700) and
340 mL (cat. no. XX4004740)
Connections Inlet: 3/8 in. tubing adapter
Outlet: accepts 1/4in. to 3/8 in. inner diameter flexible tubing
Dimensions Diameter (locking ring): 6.9 cm (2.7 in.)
Height (XX4004700): 28.1 cm (11.1 in.)
(XX4004740): 52.5 cm (20.7 in.)
Weight XX4004700: 1.1 kg (2.3 lb)
XX4004740: 1.2 kg (2.7 lb)
Materials of
construction
Top cap, barrel, filter support screen, base: stainless steel
Locking ring and wrenches: anodized aluminum
Filter O-ring and locking ring gasket: PTFE
Top cap O-ring: fluoroelastomer
Stopper: silicone
Typical Filter Flow Rates
For typical flow rates and available microporous membrane filters, go to
www.millipore.com and enter the family name (Durapore, MF-Millipore, Fluoropore,
etc. + membrane filters) in the search box.
Statement Regarding Compliance with the Pressure
Equipment Directive, 97/23/EC
EMD Millipore Corporation certifies that this product complies with the European
Pressure Equipment Directive, 97/23/EC of 29 May 1997. This product is classified
under Article 3 § 3 of the Pressure Equipment Directive. It has been designed and
manufactured in accordance with sound engineering practices to ensure safe use.
The product is accompanied by user instructions and bears markings to permit
identification of EMD Millipore Corporation as the manufacturer or authorized
representative of this product within the European Community. In compliance with
Article 3 § 3 of the Pressure Equipment Directive, this product does not bear the
CEmark.
Product Ordering Information
This section lists catalogue numbers for the Stainless Steel 47 mm Pressure Filter
Holder, spare parts, and accessories. See the Technical Assistance section for
contact information. You can purchase these products on-line at
www.millipore.com/products.
Description Qty
Catalogue
Number
Stainless Steel In-line Filter Holder, 100 mL 1 XX4004700
Stainless Steel In-line Filter Holder, 340 mL 1 XX4004740
Top cap with hose adapter 1 000205
Locking ring gasket 5 XX4004714
Filter sealing O-ring 5 XX4004716
Filter support screen 1 XX4004704
Silicone stopper, 9.5 mm (3/8 in.) hole 5 XX2004718
Replacement parts kit, 47 mm holder (includes top cap
O-rings [5], locking ring gaskets [5], filter sealing O-rings [5])
1 XX40047RK
Accessories
Filter forceps, stainless steel, blunt end (3/pk) 1 XX6200006P
PVC tubing (not autoclavable), 9.5 mm (3/8 in.) inner
diameter × 3 meters (10 ft), with 2 stainless steel clamps
1 XX6700034
Vacuum/pressure pump, 115 V, 60 Hz 1 WP6111560
Vacuum/pressure pump, 230 V, 50 Hz 1 WP6122050
Vacuum/pressure pump, 100 V, 50/60 Hz 1 WP6110060
Notice
The information in this document is subject to change without notice and should
not be construed as a commitment by EMD Millipore Corporation (“Millipore”) or
an affiliate. Neither EMD Millipore Corporation nor any of its affiliates assumes
responsibility for any errors that may appear in this document.
Technical Assistance
For more information, contact the office nearest you. In the U.S., call
1-800-221-1975. Outside the U.S., go to our web site at www.millipore.com/offices
for up-to-date worldwide contact information. You can also visit the tech service
page on our web site at www.millipore.com/techservice.
Standard Warranty
The applicable warranty for the products listed in this publication may be found at
www.millipore.com/terms (“Conditions of Sale”).
Specifications, continuedAutoclaving, continued
M12194_Rev1215.indd 2 12/22/2015 8:41:32 AM