NuAire 12 Series User manual

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Air-Jacketed DH Invitrocell CO2Incubator
Models
NU-5800 (Series 12)
NU-5800E (Series 12)
Operation and Maintenance Manual
March 2022
Revision 6
Manufactured By:
NuAire, Inc.
2100 Fernbrook Lane
Plymouth, MN 55447
Toll-Free: 1-800-328-3352
In Minnesota: (763)-553-1270
Fax: [phone]
115V 50/60Hz ONLY

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Air-Jacketed DH Invitrocell CO2Incubator
Operation & Maintenance Manual
NU-5800/NU-5800E
Table of Contents
Section No. 1..................................................General Description
Section No. 2..................................................Performance Parameters and Features
Section No. 3..................................................Models & Features
Section No. 4..................................................Test Performance & Procedures
Section No. 5..................................................Warranty Disclaimer
Section No. 6..................................................Shipments
Section No. 7..................................................Installation
Section No. 8..................................................DH Operation
8.1 ..............................................Sterility
8.2 ..............................................Humidity
8.3 ..............................................Control System Introduction
8.4 ..............................................Front Control Panel
8.5 ..............................................Rear Component Panel
8.6 ..............................................Front Component Panel
8.7 ..............................................Run Mode Operator Interactions
8.8 ..............................................Standby Mode Operator Interactions
8.9 ..............................................Diagnostic Interactions
8.10 ............................................Resetting the Electronic Software
Section No. 9..................................................Calibration
9.1 ..............................................Chamber Temperature Calibration
9.2 ..............................................Door and Perimeter Heater Adjustments Affecting Temperature Calibration
9.3 ..............................................CO2Calibration
Section No. 10................................................Maintaining Your DH Invitrocell
10.1 ............................................Chemical Decontamination
10.2 ............................................Power Loss Recovery Mode
10.3 ............................................Shutting Down the Incubator
10.4 ............................................In Filter Change Notification
Section No. 11..............................................Error Indicators & Troubleshooting
Section No. 12................................................Communication Outputs
Section No. 13................................................Electrical/Environmental Requirements
Section No. 14................................................Disposal and Recycle
Insert..............................................................Replacement Parts List
MANUAL DRAWINGS
BCD-18314 .................................................... Dimensioned Assembly NU- 5800/E
BCD-19904 .................................................... DH/D interior prep
BCD-15669 .................................................... Rear Component Panel Assembly
ASSEMBLY DRAWINGS
BCD-18316 .................................................... Front Component Panel
BCD-15177 .................................................... Electronics Panel Assembly
BCD-18318 .................................................... Sensor Bay Assembly
BCD-18315 .................................................... Tubing Diagram
BCD-18317 .................................................... Incubator Right Hinge Door Assembly
BCD-18321 .................................................... Incubator Left Hinge Door Assembly
ELECTRICAL SCHEMATICS
BCD-17791 .................................................... Electrical Schematic, NU-5800/E
BCD-18007 .................................................... Main Control Board Electrical Schematic

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DH Direct Heat
Automatic CO2Incubator
Models: NU-5800 and NU-5800E
Operation and Maintenance Manual
1.0 General Description
The NuAire Direct Heat (DH) Automatic CO2Direct Heat Incubator has been designed to provide a reliable controlled in-
vitro environment for optimum tissue cell culture growth. The chamber also provides an environment for the storage and
preservation of embryos, gametes and animal tissue cell cultures intended for research at or near body temperature. There
are five parameters that contribute to optimum growth conditions.
These are:
Humidity
Precise temperature control
Precise CO2control
Sterility
Reliability
Like all NuAire equipment, this Incubator has been designed to provide the highest quality standards of performance with
matching computer technology, precise temperature control, and CO2gas control system combining state-of-the-art
technology with years of design, quality, and manufacturing experience.
1.1 Incubator Chamber
The design and size of the DH inner chamber provides a large capacity (7 cu. ft.), and ease of use. The chamber walls are
directly heated by physically attached foil heating elements on the sides, bottom, top, and back of the chamber, providing a
temperature uniformity of +0.3°C. A space-age high-density insulation that has a high "R" rating covers the complete outer
surfaces of the Incubator inner chamber.
1.2 Incubator Closed-loop chamber sample circulation system & HEPA Filter
A continuous operating air pump draws environmental sample from the chamber and circulates it through a HEPA filter
capsule then through sensors that measure CO2 volume (as a percentage) in the chamber for monitoring and control.
1.3 Incubator Control Electronics
The NuAire Incubator Control Electronics is a state-of-the-art microcomputer-based control system specifically designed to
service the precise control requirements of the chambers environment, providing optimum programmable conditions for
culture growth. The microcomputer is “user-friendly” and provides a 5x7 LCD screen which provides operating control
parameters, status indicators, additional key operational parameters, and an imbedded touch panel to permit efficient
operator entry of data.
The microcomputer is supported with Read-Only Memory (ROM) containing executable software, Random Access Memory
(RAM) for temporary storage, and Electronically Erasable Programmable Read-Only Memory (EEPROM) for control set
points and parameters. The EEPROM provides for indefinite storage of these values during periods of power off or power
interruption (power fault-tolerant).
1.4 Incubator CO2Control
The NuAire direct heat Incubator incorporates a microprocessor-based, non-dispersive infrared CO2induction sensor.
The amount of energy received at the detector is an approximate logarithmic function of the CO2concentration in the gas
between source and detector. The wavelengths used are absorbed only by CO2making the measurement insensitive to
other components, such as water vapor. Detector linearization is performed with 32-bit digital accuracy. Advanced design
provides a very stable output minimizing drift and requiring less frequent calibration. The output is digital, alleviating errors
brought about by analog signals. Calibration of this control is accomplished by a zero & span calibration done on the sensor
and a display off- set calibration. These calibrations are accessible through the NU-TOUCH LCD located on the front of the
unit.

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1.5 Incubator Construction
The outer shell of the air-jacketed Incubator is cold-rolled steel with a powder coat paint finish. The front frame surface of
the outer shell is heated with a foil type heater directly attached behind the front perimeter opening.
The front frame perimeter heater, as well as the outer front door heater is duty cycle controlled (manually adjusted for
specific ambient conditions) to balance the heat that reaches the chamber and thus reduces the possibility of condensation
forming on the inner glass door and the inner chamber walls.
The inner chamber is 16-gauge, type 304L, polished stainless steel using crevice-free construction, which provides an easily
cleanable inert surface that does not in itself promote biological growth. In addition, all shelves, shelf supports, and guide
rails are easily removable and can be autoclaved separately if so desired.
Remember: The chamber environment is not selective. The growth environment is applied equally to all microorganisms
(specimens and contaminates) within the chamber.
1.6 Incubator Humidity
A relative humidity level of up to 90% is achieved in the Incubator by the use of a stainless-steel pan filled with distilled
water no purer than 1 megaohm, and placed on the bottom of the chamber. It is necessary to set the duty cycle of the door
and front perimeter heater in proper proportions to reduce the possibility of condensation forming on the glass inner door
and the chamber walls. It is also important to thoroughly wipe the walls and the glass door clean before adding the
humidity water pan. Condensation will occur more readily at contamination points. There is no electronic sensing and thus
no automatic control of the humidity level in the air jacketed Incubator. An air pump injects fresh air into the chamber at a
preset duty cycle to reduce condensation forming on the chamber walls and front glass door. The air injections are settable
by the user to help control condensation. (See section 8.8 for this option.)
1.7 Cabinet Ventilator Fan
An axial fan is mounted to the bottom cover panel of the Incubator and runs continuously when the unit is switched on.
This fan pulls air into the cabinet shell thru the top panel louver openings and discharges it out of the bottom of the unit.
The operation of this fan is necessary to assure accurate chamber temperature control over the 60°F to 85°F (15°C to 30°C)
ambient temperature range that the equipment may see.
1.8 Safety Instructions
These safety instructions describe the safety features of the INVITROCELL Incubator.
The incubator has been manufactured using the latest technological developments and has been thoroughly tested before
delivery. It may, however, present potential hazards if it is not used according to the intended purpose or outside of
operating parameters. Therefore, the following procedures must always be observed:
•The incubator must be operated only by trained and authorized personnel.
•For any operation of this unit, the operator must prepare clear and concise written instructions for operating and
cleaning, utilizing applicable safety data sheets, plant hygiene guidelines, and technical regulations, in particular.
owhich decontamination measures are to be applied for the incubator and accessories
owhich protective measures apply while specific agents are used
owhich measures are to be taken in the case of an accident
•Repairs to the device must be carried out only by trained and authorized expert personnel.
•Keep these operating instructions close to the unit so that safety instructions and important information are
always accessible.
•Should you encounter problems that are not detailed adequately in the operating instructions, please contact your
NuAire Representative of NuAire technical Services.

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1.9 Explanation of symbols
Symbol
Description
CAUTION:
Potential electrical hazard, only qualified person to
access.
Note: Used for important information
Biohazard
Ground Earth
Hazardous Gases!
Personal Protection Equipment Required.
Chemical Hazard
Flamable Hazard
Lead Free
Hot Surface Burn Potential
Attention accompanies information or important symbol
!
Safety alert symbol indicates a potentially hazardous
situation which, if not avoided, could result in death of
serious injury.
CAUTION
!
Safety alert symbol indicates a potentially hazardous
situation which, if not avoided, may result in minor or
moderate injury.
WARNING
!
CAUTION used without the safety alert symbol
indicates a potentially hazardous situation which, if not
avoided, may result in property damage.


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2.0 Performance Parameters and Features
•Both the interior and exterior of the DH are constructed of 18 to 16-gauge steel.
The interior is highly polished type 304 L 16 gauge stainless steel, using crevice-free construction.
All exposed edges are deburred to insure no sharp edges.
The exterior is cold rolled 16 & 18 gauge steel finished in a powder-coated polyurethane finish, which is resistant to
chemicals and easily cleaned using mild household detergents.
•The DH's microcomputer temperature control system has two temperature sensors located inside the chamber at the
back wall.
The temperature sensors compare the values to a set point and execute a differential control algorithm that energizes
a solid-state switch, supplying power to the heaters.
•Easily removable inner shelves and rails for sterilization.
•Up to 20 shelves can be placed inside the chamber (4 shelves are shipped with each DH).
•Space-age high-temperature material is used to insulate the inner chamber walls.
•Foil heaters are directly attached to the chamber walls and the top and bottom surfaces of the inner chamber.
•Most electronics, motors, pumps, and valves are fully accessible from the back of the unit.
•Access to the filters and CO₂ sensor in the sensor bay in the front of the incubator.
•A CO2sample port is provided in the center of the inner glass door to check the concentration of CO2in the chamber.
•The CO2percentage is controlled by a solid-state gas infrared sensor using a single light source with dual-wavelength
detection, providing accurate monitoring of CO2.
•Automatic recovery of the CO2level, after a 1minute door opening, to 5.0 + 0.5% is in 5 minutes on average.
•The outer door includes an internal radiant heater in order to minimize condensation on the inner glass door.
A magnetic outer door gasket helps to insure a tight seal against the cabinet.
•The inner glass door is 3/16” tempered with smooth-ground edges and seals are tight against a silicone rubber gasket.
The door latch is cam action.
A momentary switch monitors the door position, the position being opened or closed.
•All control electronics are protected with a circuit breaker that may trip at 110% of loading rating but will trip at 145%
of load rating in less than 2 seconds.
Should the circuit breaker open (pop-out button will appear), merely depress to reset.
•The Incubator has factory-installed adjustable leveling legs to compensate for uneven laboratory surfaces.

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3.0 Models & Features
3.1 Dimensions (see also Specification Drawing BCD-18314)
Overall Dimensions - inches (mm): Model NU-5800/E
Height: Exterior: 39.688 in (1008 mm)
Width: 26.750 in (680 mm)
Depth: 27.188 in (691 mm)
Foot print: 23.5 in (597 mm) wide x 20.75 in (527 mm) deep
Height: Interior: 28.500 in (723.900mm)
Width: 21.257 in (539.928mm)
Depth: 20.053 in (509.346mm)
Shelf Capacity:
Size: 19.75 Inches (502mm) x 18.75 Inches (476.5mm)
Supplied: 4 Shelves
Maximum Capacity: 20 Shelves
Maximum Weight Capacity 25 lbs. per DH shelf
125 lbs. per DH Incubator
(Make sure shelf is empty before changing position or removing.)
Water Pan:
Dimensions: Mean Length 12.0” (305mm)
Mean Width 10.0” (254mm)
Depth 1.5” (38mm)
Capacity: Maximum 2.0 Liters
Recommended Fill 1.5 Liters
3.2 Performance Parameters:
•Temperature Range: 5°C to 55°C (5°C above ambient to 30°C maximum ambient)
•Temperature Uniformity: +0.3°C @ 37°C.
•Temperature Accuracy: +0.1°C.
•CO2Range: 0.1 to 20%. (0.0 set point idles system)
•CO2Accuracy: +0.1%
•CO2Recovery: Up to 5% +0.2/-0.5 in 5 minutes average.
•Temperature Recovery: 0.12°C/minute Average
•Temperature Display Resolution: 0.1°C
•CO2Display Resolution: 0.1%
•Door and Perimeter: Proportional base duty cycle based on Temperature set point
and 0-100% manually adjustable to adapt to ambient conditions.
•Temperature Sensor Type: Precision Integrated Circuit
•CO2Control Logic: Fixed Algorithm/Manual Environmental Adaptable.
•CO2Sensor Type: Infrared single source dual wavelength
•Sensor Bay Temp Chamber Set Point +3.5 Degree C.
•Connectors on rear panel are provided for Dry contact remote alarm, RS-485 2way communication,
and 4 – 20 mA analog output signals for system performance.
Note: Performance ratings apply ONLY when the Incubator is installed and set up properly per the
instructions in the manual, and it has stabilized at all set points and is in a stable environment.
See sections 8 and 9 for proper set up.
Idle system will not inject gas and will not alarm.

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3.3 Standard Items Packed With Unit
•Four (4) stainless steel shelves
•Eight (8) stainless steel shelf brackets
•One (1) water pan
•Gas supply tube(s)
•Access port plug - vented
•One (1), 2 meter (6.5 ft.) electrical cord 10 Amp 18awg with 70°C rated jacket *
•Operation and Maintenance Manual
•Operating Instructions
*Do not replace electrical cord with an inadequately rated cord.
A factory supplied replacement is recommended.
3.4 Consult Factory for additional Accessories (Ordered Separately)

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4.0 Test Performance & Procedures
All equipment is thoroughly inspected at the NuAire Factory at the time of shipment. Quality control is maintained by
constant surveillance over the product, beginning at the receipt of purchased material and concluding with a final
inspection, which certifies the Incubator performance to the specifications. In all instances where product quality cannot
be easily assessed on the end item, the product is inspected during sub-assembly fabrication. The following test procedures
are conducted on every DH Incubator and a copy of the test report is included with each Incubator.
4.1 Visual Inspection
•Each Incubator is visually inspected to ensure that the interior is clean and free from scratches, nicks, and burrs
and that all welds, both interior and exterior are finished.
•Painted surfaces are inspected to be free of scratches, nicks, insufficient covering, and noticeable paint runs.
•The doors open and close freely without binding of the hinges.
•The gasket seals the inner glass door to the Incubator tightly across its entire opening.
•The glass door is free of scratches.
4.2 Electrical Tests
•Electrical Leakage Test
All Incubators are required to have primary-circuit filtering to meet EMC (electromagnetic compatibility) regulations.
Electrical leakage does not exceed 1.0 milliamperes.
•Dielectric Voltage – Withstand Test
1200 VDC (115-volt units) or 2000 VDC (230-volt units) is applied between dead metal parts and the hot/neutral power
source lead with no electrical breakdown using an Associated Research Model 7564SA Quadchek II, 8106 Omnia 6, or
8006 Omnia 6, or other models.
•Grounding Continuity
The resistance between the green bonding conductor of the supply cord and any dead metal part of the cabinet shall
not exceed 0.10 ohms.
4.3 Functional Tests
These functional tests are performed on every Incubator over a 48-hour final acceptance test/burn-in period.
All failures are noted, corrected, and the failed test is successfully repeated.
•Control Systems
All performance and diagnostic functions are exercised to insure proper operation of control systems,
components, and alarms.
•CO2 Control
Each Incubator is calibrated to function at a 5% CO2 level.
The concentration is checked with an independent calibrated instrument.
Each unit is monitored during the 48-hour burn-in period and only accepted with zero failures.
•CO2 Recovery
Each Incubator is exercised for CO2 recovery time at the end of the 48-hour burn-in period.
The door is opened for 1 minute to deplete the CO2.
After the door is closed, the unit shall recover to 5% +0.2 / - 0.5% within a 5-minute period.
•Temperature
Each Incubator is monitored for stable temperature control over the 48-hour burn-in period at (37°C).
•Humidity
Each unit has a default RH level intended to avoid condensation.
Door and perimeter duty cycles are determined by a Base duty cycle calculation based on the chamber
Temperature set point. Automatically adjusted in response to environmental conditions but can be manually
adjusted if needed to control condensation and tune in the chamber temperature uniformity for the environment
that the incubator is installed in.
If unit is master reset these settings will be changed to a default value of 0.0.
The factory default door and perimeter settings are: Door: 0.0, Perimeter: 0.0.

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5.0 Warranty
Details regarding product warranties can be found in the published warranty data separate from this manual and included
within the data packet send with the unit.
6.0 Shipments
NuAire, Inc. takes every reasonable precaution to assure that your Incubator arrives without damage. Motor carriers are
carefully selected and shipping cartons have been specifically designed to insure your purchase arrives safely and
undamaged. However, damage can occur in any shipment and the following paragraph outlines those steps you should
take on receipt of a NuAire Incubator to be sure that if damage has occurred, the proper claims and actions are taken
immediately.
6.1 Damaged Shipments
6.1.1 Terms are F.O.B. factory, unless stated otherwise.
Therefore, it is important to check each shipment before acceptance.
6.1.2 If there is visible damage, the material can be accepted after the driver makes a notation on the consignee's copy of
the freight bill. Then an inspection must be made to verify the claim against the carrier. This inspection is the basis
of your filing the claim against the carrier.
6.1.3 If concealed damage is found, it is absolutely necessary to NOTIFY THE FREIGHT AGENT AT ONCE, and request an
inspection. Without this inspection, the transportation company may not accept a claim for loss or damage. If the
carrier will not perform the inspection, an affidavit must be prepared stating that he was contacted on a certain date
and that he failed to comply with the request. This, along with other papers in the customer's possession will
support the claim.

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7.0 Installation
The Incubator is fastened to the base skid and it is usually the best procedure to leave the skid attached until the Incubator
is located in its approximate position to facilitate ease in handling. The base skid can then be removed by removing the
four bolts holding the cabinet to the skid. Then screw the leveling feet into the same nuts that were used to fasten the
incubator to the skid. The incubator must be a minimum of 2.0 inches above the floor. Use the 1.0” spacers provided on
the threaded stem of each leveling foot when they are installed to ensure this spacing. See BCD-18314 for dimensional and
feature information about the incubator.
It is recommended that no less than two people
are present when the incubator needs to be moved.
Examine the Incubator carefully.
INSPECT both the exterior and the interior for any transit damage before discarding the shipping crate.
7.1 Location
In locating the Incubator, consider all possible conditions that might affect its performance as well as laboratory procedures
for its intended purpose. Do not locate near heating or cooling ducts, or next to equipment that generates heat (steam
radiators, stoves, ovens, autoclaves, etc.). Avoid direct sunrays and rapidly moving air currents. These conditions adversely
affect the even heat dissipation required from the exterior surfaces of the Incubator and may cause the temperature
variation in the chamber to exceed specifications as stated in section 3.2. Since the Incubator needs even heat dissipation
on all surfaces in order to maintain an internal temperature variation of less than ± 0.2°C, a minimum of 2 inches (50mm)
must be allowed between the rear and sides of the Incubator and any walls, partitions or obstructions to facilitate adequate
convection of air around the Incubator. Confirm clearance with a tape measure if needed. Adjust the Incubator location
accordingly. In addition, the Incubator location should address access to its power cord for connection/disconnection if
necessary. For maintenance and service purposes, the control center electronics should remain accessible. Leave at least 3
ft of clearance in front for the swinging door.
7.2 Leveling
Prior to use the incubator should be leveled using a bubble level on a middle shelf in the chamber. The Incubator should
have all 4 leveling feet firmly on the bench or floor. Level from side to side and front to back. By turning the adjustable
leveling feet counter-clockwise, raises the Incubator.
7.3 Interior Chamber, Shelf & Water Pan preparation.
Chamber & Shelves
Incubator is shipped with the shelves installed. A shelf mounted water pan is also available. NuAire recommends
decontaminating all surfaces within the interior chamber, glass door, and outer door with gasket. They can be wiped down
with a disinfectant of 70 percent alcohol or a similar non-corrosive antimicrobial agent. Use only disinfectants that are
compatible with the vinyl gasket, the powder coat paint on the outer door, and the 304L stainless steel in the chamber. See
BCD-19904
Notes: Absolutely no chlorinated or halogen materials are to be used in the chamber.
NuAire does not offer any product warranty with respect to cleaning material compatibility. USE AT YOUR
OWN RISK! The information provided above is from raw material suppliers and known general source documents
for use to develop application cleaning SOP’s.
Four (4) shelves are installed in each Incubator. The shelf positions are easily changed by removing the shelf and moving
the horizontal guides to the position desired. If the shelf does not bind or disengage from the horizontal bracket it is
installed correctly. Additional shelves and shelf guides are available.
Water Pan
Place water pan in the center on the bottom of the chamber and fill with Single distilled water no purer than 1 Mega Ohm.
It is recommended to fill the pan to a maximum of about 1/2 inch below the top rim. See Sections 3.1 and 8.2 for more
specifications and operational details regarding proper maintenance instruction regarding the water.
CAUTION
!

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7.4 Electrical
The electrical supply circuit to the Incubator must conform to all national and local electrical codes.
Consult the serial-data plate, located at the front of the right side of the Incubator, for voltage, cycle, phase, and ampere
requirements before making connection. Plug the power cord securely into a grounded power source. VOLTAGE SHOULD
NOT VARY MORE THAN 5% FROM SERIAL PLATE RATINGS. Have a qualified technician check with the power source with a
properly rated voltmeter if needed. A separate branch circuit is recommended to prevent possible loss of product due to
overloading or failure of other equipment on the same circuit. A SURGE PROTECTOR IS STRONGLY RECOMMENDED to
avoid power-related faults.
7.5 Air Inlet Connection
The air inlet and filter are installed at the Factory. The filter has flexible hose that attaches to bulkhead fittings in the
bottom of the front panel and can be found under the Valance that is beneath the door. One 50mm polypropylene .3-
micron HEPA filter is connected to the hoses. The filter is labeled “Air filter”. To open the valence the outer door must be
open. Reach in under the valance and lift is for access. See BCD-18316. The air inlet on the back of the incubator is
connected to the filter by a hose at the factory and does not require installation.
CAUTION: This is a free (open air atmospheric) air supply. DO NOT connect to a pressurized air source.
7.6 CO2 Connection
High concentrations of CO2gas can cause asphyxiation!
Install Incubator in a well-ventilated area.
This Incubator is designed to be operated with CO2gas only. Connecting a flammable or toxic gas can result in a hazardous
condition. Gases other than CO2should not be connected to this equipment. CO2 gas cylinders have a UN1013 label on the
cylinder and are equipped with a CGA 320 outlet valve. Check the gas cylinder for the proper identification labels.
Do not use CO2gas cylinders equipped with siphon tubes. A siphon tube is used to extract liquid CO2 from the cylinder
which can damage the pressure regulator. Consult with your gas supplier to ensure that the CO2cylinder does not contain a
siphon tube.
7.6.1 Gas and Air supply HEPA filtering is installed at the factory
on the front of the incubator as illustrated in the picture.
Each incubator is equipped with the filters required for
the control systems available on that model.
CAUTION
!

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7.6.2 CO2Tube Connection
Included with the Incubator are a tubing kit consisting of (1) six-foot (2m) vinyl tube. Hook one end the six-foot tube
to the fitting labeled CO2 (20 psi) on the back component panel of the incubator. Then hook the other end on the
outlet of the 2-stage regulator located on the gas supply. You can locate the CO2 supply filter on the bottom right of
the front panel behind the valance at the bottom of the outer door. The filter is labeled CO2 filter. To remove the
valance the outer door must be open. Please see the Maintenance section for the proper period to change the filter.
7.6.3 CO2 Supply
•CO2of medical grade is recommended.
•A two-stage pressure regulator, Linde # 19590, or equal, is recommended.
•DO NOT USE a single stage regulator.
It will not give a stable output at 20 psi and exposes the Incubator to the gas cylinder pressure.
7.6.4 CO2Regulators
The regulator’s high-pressure stage direct from the supply cylinder must have a range of from 0 to 2000 PSI or
0 to 140 BAR. This gauge indicates actual tank pressure. The low-pressure stage should have a range of 0 to 30 PSI
or 0 to 2 BAR (100 PSI or 6 BARS maximum). This gauge will indicate the actual CO2pressure to the Incubator. Some
single stage CO2pressure regulators have two gauges. USE A TWO-STAGE REGULATOR. All NuAire Incubators use
CO2in such small quantities that precise metering of CO2input pressure is important for maximum performance.
To connect the regulator:
First: Open the CO2cylinder slightly, for an instant (this is termed “cracking the valve.”)
This will blow out dust or dirt that may have collected in the valve outlet.
BE SURE to keep your face away from the valve outlet to protect your eyes from dust or dirt.
Second: Make sure the regulator pressure-adjusting screw is released by turning it counterclockwise
until it turns freely.
Third: Attach the regulator to the cylinder valve and tighten the connection nut with a wrench.
BE SURE DISC SEAL IS IN PLACE BEFORE MAKING CONNECTION.
7.6.5 CO2Connection
Connect the CO2supply from the low-stage of the two-stage regulator to the inlet fitting located on the Incubator
back panel labeled “CO2 tank 1”. The filter should be inserted downstream of the low-stage regulator before the
inlet fitting on the Incubator as shown in the figure. Secure each connection with the clamps supplied. Observe
proper flow orientation of the filter (look for “in” or a green dot on the filter). The tubing is easily cut with a sharp
knife.
7.6.6 CO2Supply Adjustment
With the regulator OFF (i.e. fully counterclockwise), open the cylinder valve slowly usually 1 to 2 turns is sufficient.
NEVER STAND IN FRONT OR BEHIND THE REGULATOR WHEN
OPENING THE VALVE. ALWAYS STAND TO ONE SIDE.
The cylinder tank pressure should read 700 to 800 PSI or 48 to 55 BAR, more or less, depending on the temperature
of the cylinder. Next, turn the regulator’s pressure adjusting screw clockwise, until the low-pressure gauge reads
20 PSI or 1.4 BAR. The CO2connection is now complete.
Note: OSHA requires the CO2tanks to be physically restrained (i.e. via chained to wall)
to prevent accidental damage to cylinder.
7.6.7 Checking the Connections
The connections can be checked for leakage by brushing a small amount of soapy water on each one.
Observe to see if any bubbles are blown. If not, the connection is secured properly.
If it is, the cause of the leaking needs to be determined, (i.e. loose clamp or damaged hose) and corrected.
CAUTION
!

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7.7 Remove the cable tie used to secure the air pump for shipping:
7.8 Setting up the access port:
When utilizing the access port do not block or restrict the relief holes in the center of the port plug. Blocking this venting
can cause performance issues for the incubator. Cut slits or new holes in the vented plug to make passage for cables or
other items requiring access to the chamber if needed.
7.9 Correct Installation
When the Incubator is installed correctly connected to the power source it is rated for gas connections made properly, the
water pan filled, the shelves in place, and the unit is leveled. Read section 8 and follow all instructions for setting set points
at the desired value. Then calibrate the Incubator control systems as explained in section 9. If the Incubator is installed and
calibrated correctly it will meet the performance specs listed in section 3.2.
7.10 Reversing Incubator Hinges
Reversing the incubator hinges from the right to the left side SEE PTB0236 for instructions.
This requires disassembly of the incubator and should be done by a qualified service technician.

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8.0 DH Operation
Safety alert symbol indicates potentially hazardous
Situation which, if not avoided, may result in minor
Or Moderate injury.
CAUTION: A qualified technician who is familiar with the proper maintenance procedures required for this equipment,
as well as repair must perform all maintenance actions on this equipment.
The Incubator is designed to provide a sterile, constant temperature, constant CO2level and naturally humidified
atmosphere for optimum growth of tissue cell cultures and other organisms requiring this precise environment. To operate
the Incubator properly, the following parameters must be reviewed, carefully set, and/or prepared.
8.1 Sterility
The chamber environment is not selective. The growth environment is applied equally to all microorganisms (specimens
and contaminates) within the chamber. Therefore, before placing any cultures in the Incubator, the shelves and sidewall
top plenums should be sterilized. The interior sidewalls, top, bottom, door, as well as the gasket should be wiped clean
with a 70% solution of isopropyl alcohol or other disinfectants compatible with the chamber construction, to remove any
contamination. Use mild detergent to clean the exterior of the Incubator.
NOTE: NuAire does not offer any product warranty with respect to cleaning material compatibility. USE AT YOUR
OWN RISK! The information provided above is from raw material suppliers and known general source documents
for use to develop application cleaning SOP’s.
8.2 Humidity
Humidification of the Incubator chamber is achieved through the process of water evaporation (vapor water pressure) from
a stainless steel water pan placed on the bottom of the chamber. Materials of different thermal resistance (e.g., glass,
plastic) do not offer sufficient thermal recovery and are not recommended for water pan usage. Although some metals
offer better thermal coefficients than stainless steel, dissimilar metals cause electrolysis in the acid atmosphere (carbonic
acid) and should never be used or placed within the chamber.
Use only distilled or mineral-free water, no purer than 1 megaohm, in the stainless-steel pan. The water should be changed
at least once a week. FLOODING THE BOTTOM OF THE INCUBATOR IS NOT RECOMMENDED since it is difficult to change
the water weekly and almost always necessitates the use of chemicals, which are not recommended and may damage the
stainless steel. Also, it promotes condensation on the inner walls because it steals the natural convection, heat flow
through the inner chamber and condensation points occur.
Note: ABSOLUTELY NO CHLORINATED OR HALOGEN MATERIALS ARE TO BE USED IN THE CHAMBER.
Humidity recovery to 90% of original level will occur in 20-40 minutes on average after a 15-second door opening with a
water pan surface area of 120 square inches (774.2 sq. cm). Contamination in the water pan may be avoided by adding a
small amount of copper sulfate to the water pan after each Incubator decontamination. NOTE: Please contact the copper
sulfate manufacturer to determine the proper amount of copper sulfate that should be added to the water pan.
Condensation on the glass door, walls, top, or bottom of the chamber indicates an incorrect balance of door and front
perimeter heat. Both the door and front perimeter heaters operate on a duty cycle. A good starting point for these duty
cycles is the default setting for the door & perimeter heaters in a room ambient temp of 22°C (72°F) at a temperature set
point of 37.0°C. The fresh airflow that the air pump delivers to the chamber has been preset at the factory. If condensation
starts forming on the sides or back wall in the chamber, the number and length of air injections into the chamber can be
increased. (See section 8.8.1.2 for instructions.) To alleviate the condensation, increase the airflow. To increase RH in the
chamber, reduce the airflow. If airflow is decreased, some condensation may be unavoidable. The port plug with breather
holes must be used on the side port.
Note: DO NOT SEAL OR RESTRICT THE OPENING IN THE PORT PLUG.
THIS IS A PRESSURE RELIEF FOR THE CHAMBER.
564
191
ATTENTION ACCOMPANY'S
INFORMATION OR IMPORTANT
SYMBOL
POTENTIAL ELECTRICAL
HAZARD ONLY QUALIFIED
PERSON TO ACCESS
188
HOT SURFACE
BURN POTENTIAL
!
CAUTION
!

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8.3 Control System Introduction
The NuAire Incubator Control Electronics system is designed to serve the control requirements of the Incubator chamber.
Temperature and CO2levels are controlled by preset values to provide the optimum conditions for culture growth within a
chamber. Operator input is coordinated through the NU-TOUCH LCD keypad and status displays.
Figure 1 shows the various inputs and outputs of the system.
The NuAire Incubator Control Electronics is a state-of-the-art microcomputer-based system that provides:
8.3.1 Chamber control in a single electronic package.
8.3.2 Enhanced information presentation on a Nu-Touch LCD
Main Screen Continuously displays:
•Measured Chamber Temperature
•Measured CO2level in the chamber
•Current Screen ID “MAIN SCREEN”
•A mode status indicator (RUN, STANDBY or Power Loss Recovery)
•NuAire Logo button Leads to the System settings
(Or Password) menu when pressed
•Date and Time
•Air, CO2inject, door ajar status displays.
•An Alarm Status Menu
8.3.3 Active buttons on the MAIN SCREEN:
Button Description
Action when touched
Control system description
i.e.TEMP or CO2)
Brings a pop-up screen that describes the
control system that was touched
Measured value of each control system
Goes to the Environmental Set Point Screen
(Keypad to enter the password will pop up if the
password function is enabled)
NuAire Logo
Goes to “System Settings” Screen
Date & time display
Goes to screen for adjusting time and date

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8.3.4 Accessing the Incubator Control Screens
Accessing the many Control screens, performing functions and diagnostic tests, and maneuvering through the
NU-Touch LCD menu system is accomplished through the following methods:
•Touching a “graphic” Icon like the NuAire Logo in the Main Screen which access the System Settings menu,
or lead you to an Enter Password screen when a password has been established.
•Touching a “Button function” button command that is visible on the screen.
The button will have text in it indication the function you are about to perform.
The following buttons are used on the NU-5800:
An explanation of these buttons is as follows:
Save
-
This command saves the changes you have made and returns you to the calling screen or, in
some cases, back to the main menu.
Exit
-
This command exits you from the current screen and returns you to the calling screen.
Close
-
The command causes the current screen to be removed and returns back to the calling screen.
Back
-
This command returns you to the previous screen.
Start
-
This command causes a main event to begin (e.g. Decon Cycle).
Next
-
This command causes another step (screen), in a list of steps (screens) to be displayed
(e.g., Decon cycles and some CO
2
calibration screens).
Decon
-
This command begins the Decon sequence.
Cancel
-
This command will cancel the changes you have made, and in some circumstances, cancels the
“set of requests” that you have entered and returns you back to the calling screen.
(e.g., cancel in the middle of the Decon screen setup returns you to the calling screen)
•Touching a “Text” button command. The text or numerical value
will be in blue indicating that is a link similar to the computer or on
the internet. The system settings menu screen shown below is a
good example of both the Text commands and Button commands:
Note: The Main Screen is the one exception for the above
described buttons.
The green clock text is and is a link to adjusting the time.
The large white Control system measured values are also
a “second” link to the “Environmental Set-Points” menu and the control system descriptions are
a link to system description pop-up screens when touched.
Save Cancel
Exit Close
Cancel
Exit
Cancel
Next
Start

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8.3.5 Functioning accessed from the System Settings screen:
The “System Settings” menu screen can be accessed from the “Main Screen” by touching the NUAIRE logo:
Environmental Set-Points: Allows adjustment of Temperature, CO2, Door, and Perimeter heater power level
settings.
Note: You can also access the Environmental set points by touching any control system numerical value
in the MAIN SCREEN.
Performance Monitor: Views a function’s Set-Point and Measured values over the last seven days.
Service Settings: Protected by the password 9876, it facilitates Calibrations, Option settings,
and access to Diagnostics.
System Information: Views the units Model Number, Serial Number, Control board assembly (CCBA)
Software Revision and the Display board assembly (LCBA) Software Revision.
Display Settings: Brightness: Allows adjustment of the screen brightness.
Slide right to increase/left to decrease
Calibrate: Press button to access screen calibration - follow screen prompts /
target symbols.
Save: Remember to press the “Save” button after making changes or
calibrating the screen. They will be lost if “Cancel” is pressed to exit
the screen.
Set Password: A 4 digit password can be set and will protect access to Environmental Set points,
and changing the password
System Settings Screen
8.3.6 Automatic notification of abnormal situations (See Section 11 for more detail)
The red “Alarm Status” text message (button) will appear on the Main Screen
to indicate a fault within the Incubator system. An audible alarm is also
heard. The audible alarm is on for 10 seconds at the beginning of an alarm
condition and thereafter, it will be on 1 second every 30 seconds.
By depressing (touching) the Alarm Status text on the Main Screen, you now
reach the Incubator Alarm Status screen which will identify which Incubator
sub-systems are in an alarm state (Temperature, CO2, or General). You may
exit the screen or touch any of the sub-system buttons which will give you
more specific information on alarm conditions. See Section 11.0 for more
specifics on alarm conditions.