NuAire NU-1000 User manual

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ON & MAINTENANCE MANUAL
DELS NU-1000,1100,1200
CO2 WATER-JACKETED INCUBATORS
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Page 1
WATERJACI[ETED, GP AUTOFIXXB IrnATOR
mELS NU-1000, 1100, 1200
NU-l000E,1100E,1200E
WEWTION AND MAINTENANCE WAL
1.0 GENERAL DESQIIPTIgJ
The NuAire GP Autoflow water-jacketed incubator has been designed to
provide a reliable controlled environment for optirmrm product growth.
Four parameters contribute to optbun growth conditions. These are:
1. Hmidity
2. Precise temperature control
3. Sterility
4. Reliability
Like all NuAire equipent, the 8 Autoflow has been designed to provide
the highest quality standards of performance with matching microprocessor
technology and precise temperature control.
Ccmbining state-of-the-art
technology
with years of design, quality and manufacturing experience.
In order to accomplish
the foregoing objectives, the 8 Autoflow features
the following:
1.1 Esrtra large (16-gallour-WaterJacket - Each Chamber
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The outer stainless steel wall is lined with a space-age insulation
providing
an R5.0 rating, minimizing heat loss. The large
(16-gallon) water jacket utilizes water, one of nature's
best
"sinks" of heat. Its high capacity to hold heat makes it the ideal
medium to surround a
chamber in order to obtain temperature
unifoxmity. In fact, the ability of materials to hold heat, called
the specific heat, uses water as the canparative standard. The large
water jacket surrounding the chamber permits the water to circulate
within the jacket, prducing a temperature
uniformity of + 0.2O~.
The larger
the mass, the less susceptibility within the chr to
environmental fluctuations outside. It also establishes cabinet
stability for the
growth of vibration sensitive cells.
1.2
NuAire -- Inc_u@@or-Control Eleonics
The NuAire Incubator Control Electronics is a state-of-the-art
microprocessor
based control system specifically designed to service
the precise mntrol requirements
of the chambers environment,
providing optimum prcgramnable conditions for culture growth. The
functions include
a main water-jacket monitor.
The
main temperature control is proportional, which means that the
energy supplied to the water is directly proportional to the
difference between the water-jacket temperature and the setpoint.
This prevents/minimizes temperature overshoot
within the chamker and
provides transientfree temperature control.
Over-temperature differential setpoint is preset at 1°c over main
temperature setpoint.
The rest of the functions (located on the
M) provide a snall integrated package that is easily replaced
for minimum down time in the field.
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1.3 All Stainless_-Steel Conc$ructian
The GP Autof Low' s exterior is constructed of 16 gauge, type 304
stainless
steel with the interior being 16 gauge, type 304 polished
stainless steel using crevice free construction. This provides an
easily cleanable (for decontamination) inert surface that does not
Fn itself pranote biological grurwth. In addition, all shelves,
shelf supports, and guide rails are easily ranovable and can be
autoclaved to remove wntamination. - Remanber: the chamber is not
selective. The growth environment is applied to all micr*
arganisns within the interior, equally.
1.4 Humidity
Humidification of the chamber is achieved through the process of
water evaporation frm a water reservoir- placed within the
incubator.
In addition, water condensation (which actually lowers
the relative humidity within the chamber)
is prevented by having all
interior .surfaces exposed to the constaat tmrature water jacket,
or heated separately via an integrated control that regdates door
heat to maintain a condensation-free glass door. Without conden-
sation and with an easily cleanable stainless steel interior, the
probability of wntarmnation is minimized.
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NuAire, Inc., warn
without charge F.0.1
in its equipnent wil
satisfaction of th
=Id. Provided thz
properly identified .
Factory installed
extent guaranteed 1
apply to any portiol
this warranty shou
me nature of the
serial and &el nuni
NuAire, Inc. WarraQt!
factory or furnish w
five years after .
ccmpany to have been
-+
This . warranty shall
has been subject
using additives in
installation
.or serv
j-
If the serial nmk
be illegible, the wa
The warranty is for
assignable
or trans
contact
NuAire, Inc
accunpany
all retur
will be returned, re
mts that it will repafr F.O.B. its factory or furnish
3. its factory, a similar part to repbe any mterial
thin 18 mnths after the date of sale if proved to the
e ccmpany to have been defective at the time it was
~t all parts claimed defective shall be returned,
to the cunpany at its factory, charges prepaid.
&pent
or accessories are warranted only to the
by the original manufacturer. This wamaaty shall not
a of the equipent modified by the user. Claims under
Id be directed to NuAire, Inc. setting forth in detail
defect, the
date of the initial installation and the
ber of the equipnent.
3 the water jacket and will repair or replace (FOB its
ithout charge FOB its factory) the water jacket within
the date of sale, if proved to the satisfaction of the
defective
at the time it was sold.
not apply
to any NuAire product or part thereof which
to misuse, abuse, filling the water jacket improperly,
water jacket, accident, shipping damage, improper
ice, or damage by fire, flood or acts of Gcd.
r of this product is altered,
removed or defaced as to
mnty shall be null and void in its entirety.
the
sole benefit of the original purchaser and is not
ferable. Prior to returning any item, for any reason,
. for a Return Authorization Number. This number must
ns. Any product shipped to NuAire without this number
fused shipnent or collect freight.
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Page 11
The GP Autoflow is fastened to a base skid and it is usually the best
procedure
to leave the skid attached until the autoflow is located in its
approximate position, to facilitate ease in handling. The base skid can
then be. removed by removing the four bolts holding the cabinet to the
skid. Emnine the GP Autoflow carefully. Inspect both the exterior and
the, interior of the GP Autoflow for any transit damage before discarding
the shipping crate,
(see Section 6.1.3).
FRCTORY INSTRUED 2
\ LEG LEVELERS (Y)
L1/2-13 x 2' HD(
HEAD BOLT(+)
Wtion In locating the GP Autoflow, consider all possible
conditions
that might affect its performance as well as laboratory
procedures for
its intended purpose. lb not locate near heating or
cooling ducts, or next
to equipnent that generates heat (steam
radiators, stoves, ovens, autoclaves,
etc.). Avoid direct sun rays
and rapidly moving air currents. The GP Autoflm needs wen heat
loss on
all surfaces in order to maintain an internal temperature
variation of
less than 0.2 degrees C. As a result, a minimum of 2
inches must be allowed between the rear and sides of the GP Autoflw
and any
walls, partitions or obstructions to facilitate adequate
convection
of air around the GP Autoflow's water jacket. For
mintenance/service purposes the top cover containing the
electronics should remain accessible.
7.2 Leveling The GP Autoflow should be leveled prior to filling with
water,
and should rest firmly on the bench or floor. Uneven water
levels may cause false ADD WATER indications on the front panel as
well as to affect the water circulation paths within the water
jacket, which could cause condensation on the walls of the chamber.
Leveling feet are provided for this purpose, factory installed inb
the base of the GP Autoflow. Turning the adjustable leveling feet
counterclockwise raises
the GP Autoflow. The leveling feet height
&mild be approximately 1/4 inch below the GP Autoflow base.
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Page 13
Decontamination and Shelf Installation Before installation of the
shelves, NuAire recamends decontamination of all surfaces within
the interior chamber, glass door and outer door with zasket. The
can be wiped down with a disinfectant of 70 percent alcohol or
similar noncorrosive antimicrobial agent. Provided with each
autoflow are four shelves. The shelves are easily installed per
Drawing ACD-02177 by attaching the guide supports to the stainless
steel pins in the interior of the chamber. Additional shelves and
shelf
guides are available.
Electrical
The electrical supply circuit to the incubator must
conform to all national and local electrical codes. Consult the
jncubatork serial-data plate for voltage, cycle, phase and ampere
requirements before
making connection. Plug the power cord securely
into grounded outlet. VOLTAGE SHOULD NOT VARY MORE TWN 5% !?ROV
SERIAL PLATE RATINGS. A separate branch circuit is recarmended to
prevent possible loss of product due to overloading or failure of
other equipnent on the same circuit.
A SURGE PROTECTOR IS STRONGLY
RE(XMMENDED TO AVOID POWER RELATED FAULTS.
Precaution for GP Autoflw Filling To prepare the GP Autoflow for
filling, turn on main pwer switch located on backpanel and set mode
switch to PROGRAM. The digital indicator should light up as well as
the ADD WATER light.
Filling and Draining the Water-Jacket The fill port plug is lccated
on the front top left side behind
the exterior door. Install the
fill port fitting. Place the tubing over the adaptor and connect
the other end to either a funnel or serrated tap. USE SINGLE
DISTILLED OR DEMINERALIZED WATER to fill the water jacket. NO PURER
TH4N 5 MGOHhl WATER SHOULD BE USED.
lCvnile the unit is on, fill the
water jacket until the ADD WATER lighr; mrns off.
Add an additional
1
to 2 liters of water, renove the tube adaptor and replace the fill
port plug
CAUTION - USE SINGLE DE-IONIZED OR
DISTILLED WATER ONLY
SAFETY OVERFLOW PORT
CAUTION
BE SURE TO WSITION ANTI LEVEL THE INCUBATOR GP AUTOrm3W
DO NOT OVGRFILL,
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Page 14
The water jacket requires no anti-bacterial agents. .The GP Autoflm
already incorporates a copper tube producing copper sulfate which
eliminates bacterial growth within the water jacket. AEWLUTELY
CHLCJRINATED OR HALM;EN MATERIALS ARE TD BE USED IN THE WATER JACKET.
A safety over-fill port is located next to the fill port plug so if
overfill does occur, the water will be relieved through the port.
The water jacket can be drained frm the drain plug located on tbe
bottom left side. The drain uses a ball valve to control the drain
water.
If the valve stem is horizontal the drain is closed, and if
it is vertical the drain is open. A safety plug must be removed
prior to draining, located on the bottun of the ball valve. It als,
insures no leakage will occur.
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Page 16
The NuAire Incubator Oontrol Electronics system is designed
to service the control requirements of the GP Autoflow
incubator chamber. Temperature is controlled to provide
optimum conditions for culture growth within a chamber.
Operator input is coordinated through the control panel and
status displays. Figure 1 shows the various inputs and
outputs of the systan.
The NuAire Incubator Control Electronics is a state-
of-theart microprocessor based system that provides:
8.3.1 Single chamber control in a single electronic package
8.3.2 Enhanced infomation presentation of the following:
* Qanber Temperature (setpoint and actual)
* Door Heater (percentage time on)
* Water-Jacket Heater Status
* Water Jacket IAw Water Status
8.3.3 Simplified operator controls. The control panel is
operated using three rocker switches as follows:
* Mode Switch - Run - run with alarm
Progrm Standby
Silence - Run without alarm
* Chamber Environment Display - Temperature Display
Door Temp
* Chamber Environment Control - Up
Down
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