NuAire NU 4500 User manual

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Guide To Components
Air Inlet Filter
Circuit Breaker
On/Off Switch
Front Panel
Fill Port
Drain Valve
Shelf Assembly
Water Pan
Magnetic Door Switch
Outer Door
Inner Glass Door
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2.0 Control Panel and Indicators
HEAT JACKET Indicates Main Heater ON
DOOR AJAR Inner Glass Door Open
LOW WATER Add Water to Jacket
INJECT C02 Indicates Injection of C02
C02TANK
1 Indicates C02Tank in Use
C02Tank 2 (Option) Indicates C02
Tank in Use
ALARM Fault Occurrence
[SEL]
Select key allows selection of parameters on display
UP arrow key allows increase of parameter values
DOWN arrow key allows decrease of parameter values
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SETUP (m Mode Switch - Indicates Incubator
is Running or In Setup.
RUN Mode Indicators
Indicates Present Chamber Temperature
Indicates Present Chamber C02Percent
(Option) Indicates Present Chamber Humidity Percent
RUN Mode Fault Indicators
Blinking Green LEDNext to Display Indicates Chamber
Temperature Fault.
Blinking Green LEDNext to Display Indicates C02 Percent
Fault.
Blinking Green LEDNext to Display Indicates Humidity Percent
Fault.
All Displays Blinking Indicate Power Interruption.
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3.0 US Autoflow Start-up
After your US Autoflow Incubator has been unpacked, positioned and leveled, proceed with the following check list.
1. Check for standard items packed with unit.
(4) Stainless steel shelves
(8) Stainless steel shelf brackets
(4) Stainless steel shelf bracket supports
(1)Full size water pan with supports
(I)Fill port adapter
(1)Fill port plug
(1) Gas tube with filter
(1)
6 feet (2M), fill tube
(1) Electrical Cord
8 foot (2.5m)
(1) Operation and Maintenance Manual
(I)Operating Instructions
2.
Install shelf brackets, shelves and water pan. Connect incubatorto an appropriategrounded power source
via the backpanel power cord. Use a dedicated laboratory circuit for the power supply. A SURGE
PROTECTOR IS STRONGLY RECOMMENDED to avoid power-related faults.
Turn mode switch on front panel to setup,
turn on incubator via the odoffswitch on the backpanel.
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3. Fill incubator water-jacket with SINGLE distilled or de-ionized water. No purer than 5 MEGOHM water should
be used. Use enclosed tubing and fill port adapter to fill water-jacket until the low water light on the front panel
turns off. Add an additional
1 to 2 liters of water and install fill port plug. ABSOLUTELY NO
CHLORINATED ORHALOGEN MATERIALS
ARE TOBEUSED IN THEWATER-JACKET.
4. Attach
C02 gas supply to the CO2 inlet on the incubator backpanel. Use a TWO-STAGE REGULATOR and set
the output pressure to approximately
20 PSI.
5.
Attach air inlet tubingkilter to the air inlet fitting port on the incubator back panel.
6.
Enter temperature and C02 percent setpoints (see section 4.0).
7.
Once parameter setpoints are entered, turn mode switch to run and let incubator stabilize for 24 hours. The C02
percent will indicate "dely" until the incubator reaches within 2' of setpoint temperature.
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4.0 Setpoint Parameter Entry
Setpoint values are entered by pressing the "SEL" key tothe desired parameter indicator. The value of the
selected parameter will be shown in the display in the form
"XX.X". To enter a setpoint, perform the following:
Chamber Temperature
Turn Mode Switchto Set Up
Press [SEL]to Indicate Green LED Next to Chamber Temperature Display
. Press + or + to Indicate Desired Temperature
. Turn Mode Switch Back to Run
CO? Percent
. Turn Mode Switch to SetUp
Press [SEL] to Indicate Green LEDNext to C02Percent Display
Press + or + to Indicate Desired C02Percent
Turn Mode Switch Back to Run -
Humidity RH (Optional)
Turn Mode Switch to Set Up
Press [SEL]to Indicate Green LEDNext to Humidity Percent Display
Press + or to Indicate Desired Humidity Percent
Turn Mode Switch Back to Run
NOTE: If an entry error is made, re-enter data.
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5.0 Chamber Temperature Calibration
The US Autoflow's TEMPERATURE CALIBRATION MUSTBE PERFORMED WITHIN 1°C OF THE
PLANNED OPERATING TEMPERATURE. Normally, 37.0°C is the most common setpoint. With the incubator
operating at its setpoint, place a mercury glass thermometer in a glass beaker filled with water or through an inverted
Styrofoam cup resting on a shelf in the middle ofthe chamber. Do not place the glass beaker on the bonom of the
incubator chamber. Placing the thermometer in the middle of the chamber will givethe most accurate results for
calibration. Allow the thermometer
2 hours to stabilize.
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When the temperature is stabilized atthe operating temperature, perform the following calibration procedure.
Turn Mode Switch to Run
Press [SEL] to indicate green LED next totemperature display
Press and hold one + key for four seconds, display blinks
. Press + or key to indicate sametemperature asthermometer
Press [SEL] key to complete calibration
The chamber temperature calibration is complete. Let unit stabilize for
8 to 12 hours. If the chamber temperature (actual
thermometer) still does not match the display, perform the above procedure again. In some cases it might be necessary to
calibrate several times to achieve a stable condition due to ambient conditions of temperature and humidity within the
laboratory.
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6.0 Door Temperature Calibration
The US Autoflow's inner glass door temperature calibration is best accomplished by running the incubator 24
hours with the water pan in place and perform the following calibration sequence if required.
Open the incubator door and look for general condensation. Somecondensation on the glass door is desirable as an
indication of adequate humidity in the chamber. Typically one to two inches of condensation in the corners of the glass
door indicates a properly calibrated door heater. However, if calibration is required, simply perfom the procedureas
stated below. The door heater operates as a percentage
ONlOFF (0 is off, 100 is full ON). Typically, 40 to 60 percent is
the most effective setpoint range.
Turn Mode Switch to Set Up.
Press [SEL]to indicate green LED next to temperature display.
Press both +or J, keys simultaneously, display "dor"1door heat % on (XX).
Press + or J, key to desired door heat % on.
Press [SEL]to complete the calibration.
Turn Mode Switch back to Run.
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7.0 C02 Control Calibration
C02Control Calibration can be performed any time the C02fyrite measurement doesn't correlate to the front
panel display. However, this calibration SHOULDNOT
BE PERFORMED MORE THAN ONCE PER WEEK. Sensor
calibration should be performed if the C02fyrite measurement doesn't match the display within
20.3 percent after one
week. Before doingthe following calibration, check and change, if necessary, the incubator in-line filter found within
the control center.
When unit has stabilized at the operational temperature and C02percentage, take a C02 fyrite measurement and, if
necessary, perform the following:
Turn mode switch to RUN.
0
Press [SEL] to indicate green LED next to CO2% display.
Press and hold one + key for four seconds, C02display blinks (clears all previous offsets)(compare the display
C02to the C02fyrite measurement. If these two readings have a difference less than
2.0 percent, proceed to enter
the fyrite value. If the difference is greater than 2.096, proceed to C02sensor calibration, Section 8.0)
Press + or & key to indicate same C02%asfyrite measurement.
Press [SEL] to set current value and exit calibration.
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8.0 C02 Sensor Internal Calibration
C02sensor front panel calibration should only be performed when required due to reoccurring error indicators.
The CO2 sensor has two calibration points, a zero point (ambient C02percentage) and a span point (high C02
percentage). The following calibration procedure should be performed ONLY after the chamber temperature is stabilized
and the CO? sensor has been operating at the stabilized temperature for
2 hours. If the incubator has been in operation
for a year or more, in line filter replacement may be necessary. After the above conditions are satisfied, proceed with the
following:
%
Step 1 : Remove control center side panel.
f:z.
Step 2: Remove sensor housing via (2) thumbscrews.
Step
3: Check and replace, if necessary, in-line filters
Step
4: Replace sensor housing and secure it
Step
5: Turn mode switch to setup
Press [SEL] to indicate green LED next to Cot%display.
Press both @ and & keys simultaneously, display blinks "dor".
Step
6: Open inner glass door and evacuate chamber for 1 minute. Close door and allow chamber
environment to stabilize for
1 minute.
OM0069 13
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Step 7: Zero C02Sensor
Press + key, display blinks "ZEr" and a positive or negative number.
Locate the access panel on the sensor housing and open it.
Open the front panel by opening outer door and quickly tap on left side of front panel.
Locate the ZERO POT on the C02sensor and adjust with blue trim pot tool provided until the C02
displayed window reads 0 k.005V (Allow
30 seconds between adjustments for sensor to stabilize).
Step
8: Span C02Sensor
Press + key, display blinks "SPn"and the C02percent.
Allow the unit to self inject C02for 90 seconds. Wait an additional minute or until the display CO2
percent reading is stable. The C02inject green LED will indicate C02flow into the chamber during the
90 second automatic C02injection.
Step
9: Fyrite the incubator to determine correct C02percentage.
Locate the SPAN POT on the C02sensor control board (see Figure
2) and adjust with
trim pot tool until the display C02percent is the same asthe fyrite C02percentage
(Allow 30 seconds between adjustments for sensorto stabilize.)
Step 10:Open inner glass door and evacuate chamber for
1 minute. Then, close the door.
Press + key to complete the calibration.
Replace sensor access and control center side panel.
Turn mode switch back to run.
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Allow unit to run and stabilize for 2 hours. Check calibration with the fyrite. Compare the display C02
percent to your fyrite measurement. If these two readings have a difference greater than 0.5%, repeat
above procedure beginning with Step
5. Ifthese two readings have a difference of less than 0.5%,
perform the C02 control calibration procedure in Section 7.0.
INTERFACE CONNLCTUR
LAW FLOW VALVE
Figure 2
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9.0 Relative Humidity Calibration (Option)
Relative humidity calibration can be performed anytime if the relative humidity option has been installed. The
relative humidity sensor can be calibrated from a known source of humidity within the incubator chamber. Typically, the
water pan is used because it has a known humidity level of96 percent after one hour in the chamber.
A calibration
sensor may also be used if it's available.
The calibration procedure isa two stepprocess. The first step istoperform
an RH control calibration. The second
step,
RH sensor calibration, is only required if the RH control calibration cannot be performed.
When unit is stabilized at the operational temperature,
C02 percentage and water pan in place, perform the following
calibration procedure:
RH CONTROLCALIBRATION
Turn Mode Switch to RUN.
Press [SEL]to indicate green LED next to RH % display.
Press and hold + arrow key for four seconds, RH % display blinks (clears all previous offsets)(If the RH %
display is between 93 to 99 percent, proceed to next step. If the RH % display is not between 93 to 99 percent,
proceed to
RH sensor calibration below).
Press + or & arrow key to indicate 96 percent or known humidity percent.
Press [SEL] to setcurrent value and exit calibration.
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,
RH SENSOR CALIBRATION
Since the location ofthe humidity sensor board is difficult to reach. The calibration method described below, removes
the humidity sensor board from the rear wall and mounts it to a calibration fixture next to the side access pon of the
chamber to allow the calibration to be preformed outside the chamber.
Step
1 : Remove RH sensor from upper wall of chamber.
Step 2: Attach RH sensor cable extension and calibration
fixture. Be sure the
RH sensor adjustment pot is
accessible through the side access port.
Step
3: Close incubator doors, and, with the water pan in
place. let incubator stabilize for a minimum of one hour.
Step
4: When operational temperature and C02percentage have
stabilized with the water pan in place, perform the
following calibration procedure:
s3
Turn mode switch to RUN
Press [SEL] to indicate green LED next to RH%
vm C*
Ri WUIlIM rUM
display. Press and hold + arrow key for four seconds
until display starts blinking (clears all previous offsets) adjust RH sensor pot through side access port. Adjust
pot counter-clockwise to increase display orclockwise to decrease display. The goal is to achieve
96% or a
known measurement.
Press [SEL] to set current value and exit calibration.
Allow incubator to stabilize for
2 hours and repeat above steps if necessary. When calibration is complete,
return RH sensor to original position.
OM0069 17
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10.0 Maintaining Your US Autoflow
US Autoflow Chamber
The chamber maintenance is up to the discretion of the owner and the extent of cleanliness and sterility desired. The
shelves and bracket supportsare all removable and autoclavable. The interior should be wiped down with an appropriate
disinfectant such as
70% ISOPROPYL ALCOHOL orequivalent. DONOT USE ANY CHLORINATED OR
HALOGEN MATERIALS
IN THE CHAMBER. The humidity pan should also be sterilized and the water changed
regularly to assure sterility. A small amount of copper sulfate may be added tothe humidity pan to inhibit bacterial
growth.
US Autoflow Water-Jacket
The water-jacket requires no anti-bacterial agents. TheUS Autoflow already incorporates a copper tube producing
copper sulfate which eliminates bacterial growth within the water-jacket. DONOT USE ANY CHLORINATED OR
HALOGEN MATERIALS IN THE WATER-JACKET.
C02Supply Filter (50mm In-Line,
Dry)
The C02 Supply Filter should be replaced every fifth empty C02tank or when the filter is visibly discolored (yellow-
brown). Note direction of flow
(IN is labeled on one side of filter) when replacing filter.
Air Inlet Filter (50mm In-Line,
Dry)
The Air Inlet Filter is located on the backpanel. The purpose of the filter is to cleanse the room air which is drawn into
the chamber, via the pump, for three seconds everyminute, assuring the proper amount of oxygen is available to the
cultures. The air inlet filter should be replaced every six months.
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C02SensorFilter (Capsule In-Line, Wet)
The C02Sensor Filter should be replaced EVERY TWOYEARS to assure optimum performance. A visual check
should be performed during the
C02 sensor calibration to assure filter integrity. Remove sensor housing cover to
perform visual check. A similar procedure should be followed with the
RH filter as well (option).
RH Reservoir
THE
RH RESERVOIR REQUIRES NO ANTI-BACTERIAL AGENTS. The RH Reservoir is filled using single
distilled or de-ionized water. NO PURER THAN
5 MEG OHM WATER. Do not use any chlorinated or halogen
materials in the RH Reservoir.
. \
CN SFlhlR FIILTER
.lM%U.E IN-LIK. LET1
CLEAR RESERVIOR
1HONLTOR LEVEL)
RH OPTION RESERVIOR FILL PORT
OM0069
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11.0 Error Indicators and Troubleshooting Guide
Step 1: NOTE ALL ERROR INDICATORS. When the incubator is running, any and all red oryellow LEDs indicate an
error.
Step
2: CLEAR ERROR INDICATORS. Error indicators can be cleared by turning mode switch to setup and back to
run.
Step
3: MONITOR RE-OCCURRENCE OF ERROR INDICATORS. If re-occurrence of the error indicator is
immediate or daily, use guide below to correct the situation.
Error Indicator Troublesbootine Guide
CHAMBER TEMPERATURE
TEMPERATURE
1-
ALARM
Indicates: Chamber temperature has exceeded
allowable range or incubator not heating.
0
Temp. sensor (chamber orjacket) are
faulty orout of calibration.
Correction: Perform temperature sensor calibration.
Check or replace fuse on main control
board.
Check or replace main control board.
20
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I I I
C02PERCENT
-
ALARM
RH PERCENT
HUMIDITY %
III
u
ALARM
Indicates: Backup C02control system is in effect.
Turning the mode switch to setup and back
to run will clear it and allow the C02
control system to resume as normal.
0
Reoccurring indicator is due to the C02
percentage value exceeding its allowable
range.
Correction: Check C02gas supplyto unit.
-If display over setpoint, reduce gas cylinder
regulator pressure.
-If display under setpoint, increase gas cylinder
regulator pressure.
0 Replace in-line filters.
Perform C02sensor and control
calibration using fyrite instrument.
Indicates:
RH value has exceeded allowable range
Usually due to a low water pan.
Correction: Fill water pan.
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