NuAire NU-8625 User manual

Water-Jacketed Automatic CO2Incubator
Models
NU-8600, NU-8600E
NU-8625, NU-8625E
NU-8631, NU-8631E
NU-8645, NU-8645E
Operation and Maintenance Manual
September 2022
Revision 9
Series 3
Manufactured By:
NuAire, Inc.
2100 Fernbrook Lane
Plymouth, MN 55447
Toll-Free: 1-800-328-3352
In Minnesota: (763)-553-1270
Fax: [phone]

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Water-Jacketed Water Jacket In-VitroCell CO2Incubator
Operation & Maintenance Manual
NU-8600, NU-8600E
NU-8625, NU-8625E
NU-8631, NU-8631E
NU-8645, NU-8645E
Table of Contents
Section No. 1..................................................General Description
Section No. 2..................................................Performance Parameters and Features
Section No. 3..................................................Models & Features
Section No. 4..................................................Test Performance & Procedures
Section No. 5..................................................Warranty Statement Notice
Section No. 6..................................................Shipments
Section No. 7..................................................Installation
Section No. 8..................................................Water Jacket Operation
8.1 ..............................................Sterility
8.2 ..............................................Humidity
8.3 ..............................................Control System Introduction
8.4 ..............................................NuTouch Display Screen
8.5 ..............................................Rear Component Panel
8.6 ..............................................Run Mode Operator Interactions
8.7 ..............................................Standby Mode Operator Interactions
8.8 ..............................................Front Component Panel
8.9 ..............................................Diagnostic Interactions
8.10 ............................................Resetting the Electronic Software
Section No. 9..................................................Calibration
9.1 ..............................................Chamber Temperature Calibration
9.2 ..............................................Door Heater Adjustments Affecting Temperature Calibration
9.3 ..............................................Setting Air Injections
9.4 ..............................................CO2Calibration
9.5 ..............................................O2Calibration
Section No. 10................................................Maintaining Your Water Jacket In-VitroCell
10.1 ............................................Chemical Decontamination
10.2 ............................................Power Loss Recovery Mode
10.3 ............................................Shutting down the incubator
10.4 ............................................Automatic Filter Change Notification
Section No. 11................................................Error Indicators & Troubleshooting
Section No. 12................................................Communication Outputs
Section No. 13................................................Electrical/Environmental Requirements
Section No. 14................................................Disposal and Recycle
Insert......................................................Replacement Parts List
MANUAL DRAWINGS
BCD-18002 .................................................................. Dimensioned Assembly NU-8600
BCD-19905 .................................................................. Interior prep
BCD-15170 .................................................................. Rear Component Panel Assembly
ASSEMBLY DRAWINGS
BCD-18476 .................................................................. Front Component Panel
BCD-18477 .................................................................. Sensor Bay Assembly
BCD-19455 .................................................................. Right Hand Door Assembly
BCD-19456 .................................................................. Left Hand Door Assembly
ELECTRICAL SCHEMATICS
BCD-18364 .................................................................. Electrical Schematic
BCD-18007 .................................................................. Main Control Board Electrical Schematic

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Water Jacket
In-VitroCell CO2Incubator
Model: NU-8600, NU-8600E
NU-8625, NU-8625E
NU-8631, NU-8631E
NU-8645, NU-8645E
Operation and Maintenance Manual
1.0 General Description
The NuAire Water Jacketed Incubator has been designed to provide a reliable controlled In-vitro environment for optimum
tissue cell culture growth. The chamber also provides an environment for the storage and preservation of gametes and animal
tissue cell cultures intended for research at or near body temperature.
There are five parameters that contribute to optimum growth conditions, they are:
Humidity
Precise temperature control
Precise CO2control
Sterility
Reliability
Like all NuAire equipment, this Incubator has been designed to provide the highest quality standards of performance with
matching computer technology, precise temperature control, and CO2gas control system combining state-of-the-art technology
with years of design, quality, and manufacturing experience.
1.1 Extra Large (18 to 20 gallon) Water-Jacket - Each Chamber
The outer stainless-steel wall is lined with a space-age insulation providing a R5.0 rating, minimizing heat loss. The large 20-
gallon (75.7 liters) Water-Jacket utilizes water, one of nature’s best "sinks" of heat. Its high capacity to hold heat makes it
the ideal medium to surround a chamber in order to obtain temperature uniformity. In fact, the ability of materials to hold
heat, called the specific heat, uses water as the comparative standard. The large water-jacket surrounding the chamber
permits the water to circulate within the jacket, producing a temperature uniformity of 0.2C. The larger the mass, the
less susceptible the environment within the chamber is to environment fluctuations outside. It also adds cabinet stability
for the growth of vibration sensitive cells.
1.2 Incubator Closed loop chamber sample circulation system & HEPA Filter
A continuous operating air pump draws environmental sample from the chamber and circulates it through a HEPA filter
capsule then through sensors that measure CO2volume (as a percentage) in the chamber for monitoring and control.
1.3 Incubator Control Electronics
The NuAire Incubator Control Electronics is a state-of-the-art microcomputer-based control system specifically designed to
service the precise control requirements of the chambers environment, providing optimum programmable conditions for
culture growth. The microcomputer is “user-friendly” and provides a 5x7 LCD screen which provides operating control
parameters, status indicators, additional key operational parameters, and an imbedded touch panel to permit efficient
operator entry of data.
The microcomputer is supported with Read Only Memory (ROM) containing executable software, Random Access Memory
(RAM) for temporary storage, and Electronically Erasable Programmable Read Only Memory (EEPROM) for control set
points and parameters. The EEPROM provides for indefinite storage of these values during periods of power off or power
interruption (power fault tolerant).
1.4 Incubator CO2Control
The NuAire direct heat Incubator incorporates a microprocessor-based, non-dispersive infrared CO2induction sensor.
The amount of energy received at the detector is an approximate logarithmic function of the CO2concentration in the gas
between source and detector. The wavelengths used are absorbed only by CO2making the measurement insensitive to
other components, such as water vapor. Detector linearization is performed with 32-bit digital accuracy. Advanced design
provides a very stable output minimizing drift and requiring less frequent calibration. The output is digital, alleviating errors
brought about by analog signals. Calibration of this control is accomplished by a zero & span calibration done on the sensor

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and a display off- set calibration. These calibrations are accessible through the NuTouch LCD located on the front of the
unit.
1.5 All Stainless-Steel Construction
The exterior is constructed of 16-gauge, type 304L stainless steel with the interior being 16-gauge, type 304L polished
stainless steel using coved corner construction, which provides an easily cleanable (for decontamination) inert surface that
does not promote biological growth. All exposed edges are deburred to insure no sharp edges. The exterior is finished in a
textured polyurethane finish, which is resistant to chemicals and easily cleaned using mild household detergents.
In addition, all shelves, shelf supports, and guide rails are easily removable and can be autoclaved to remove
contamination.
Remember: The chamber environment is not selective. The growth environment is applied equally to all microorganisms
(specimens and contaminates) within the chamber.
1.6 Incubator Humidity
All Model(s)
A relative humidity level of up to 90% is achieved in the Incubator by the use of a stainless-steel pan filled with distilled
water no purer than 1 mega ohm, and placed on the bottom of the chamber. It is necessary to set the duty cycle of the
door heater in proper proportions to reduce the possibility of condensation forming on the glass inner door and the
chamber walls. It is also important to thoroughly wipe the walls and the glass door clean before adding the humidity water
pan. Condensation will occur more readily at contamination points. There is no electronic sensing and thus no automatic
control of the humidity level in the Water Jacketed Incubator. An air pump injects fresh air into the chamber at a preset
duty cycle to reduce condensation forming on the chamber walls and front glass door. The air injections are settable by the
user to help control condensation. (See section 8.8 for this option.)
1.7 O2Display and Control (NU-8631)
The Incubator has an available optional Oxygen display and control system. The system controls from 21% (ambient level)
down to 0.5%. The recovery time from ambient to 5% 2% is typically less than 15 minutes. The accuracy is 0.25% from
any given set point.
Oxygen sensor is a type, which generates logarithmic signal based on O2content in the chamber. The sensor is unaffected
by CO, H2and various acidic gases such as CO2, etc.
RH Display (NU-8625 and NU-8645)
These Incubators display RH. The system does not control RH, it is just a monitor.
1.8 Safety Instructions
These safety instructions describe the safety features of the In-VitroCell Incubator.
The incubator has been manufactured using the latest technological developments and has been thoroughly tested before
delivery. It may, however, present potential hazards if it is not used according to the intended purpose or outside of
operating parameters. Therefore, the following procedures must always be observed:
• The incubator must be operated only by trained and authorized personnel.
• For any operation of this unit, the operator must prepare clear and concise written instructions for operating and
cleaning, utilizing applicable safety data sheets, plant hygiene guidelines, and technical regulations, in particular.
oWhich decontamination measures are to be applied for the incubator and accessories?
oWhich protective measures apply while specific agents are used?
oWhich measures are to be taken in the case of an accident?
• Repairs to the device must be carried out only by trained and authorized expert personnel.
• Keep these operating instructions close to the unit so that safety instructions and important information are
always accessible.
• Should you encounter problems that are not detailed adequately in the operating instructions, please contact your
NuAire Representative or NuAire technical Services.

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1.9 Explanation of symbols
Symbol (in text box)
Description (in text box)
Safety alert symbol indicates a potentially hazardous
situation which, if not avoided, could result in death of
serious injury.
Safety alert symbol indicates a potentially hazardous
situation which, if not avoided, may result in minor or
moderate injury.
CAUTION
CAUTION used without the safety alert symbol indicates a
potentially hazardous situation which, if not avoided, may
result in property damage.
Potential electrical hazard,
only qualified person to access.
Note:
Biohazard
Ground, Earth
Hazardous Gases!
Personal Protection Equipment Required.
Chemical Hazard
Flammable Hazard
Lead Free
Hot Surface
Burn Potential

CAUTION
!
WARNING
!

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2.0 Performance Parameters and Features
•Each chamber’s water-jacket holds 18 to 20 gallons (up to 75.7 liters) of water that, in conjunction with the
microcomputer control system, provides an interior chamber temperature uniformity of 0.2C at 37.0C.
•The microcomputer temperature control system has two temperature sensors located inside the chamber at the back
wall. The temperature sensors compare the values to a set point and execute a differential control algorithm that
energizes a solid-state switch, supplying power to the heaters.
•Easily removable inner shelves and rails for sterilization.
•Up to 16 shelves can be placed inside the chamber (4 shelves are shipped).
•Space-age high-temperature material is used to insulate the Water Jacket walls.
•Most electronics, motors, pumps, and valves are fully accessible from the back of the unit.
•A CO2sample port is provided in the center of the inner glass door to check the concentration of CO2in the chamber.
•The CO2percentage is controlled by a solid-state gas infrared sensor using a single light source with dual-wavelength
detection, providing accurate monitoring of CO2.
•After a door opening the CO2level automatically recovers to 5.0 + 0.2/- 0.5% within a 5-minute period on average.
•The outer door includes an internal radiant heater in order to minimize condensation on the inner glass door.
A magnetic outer door gasket helps to insure a tight seal against the cabinet.
•The inner glass door is 3/16” tempered with smooth-ground edges and seals are tight against a silicone rubber gasket.
The door latch is cam action. A momentary switch monitors the door position, the position being opened or closed.
•All control electronics are protected with a circuit breaker that may trip at 110% of loading rating but will trip at 145%
of load rating in less than 2 seconds.
NOTE: Should the circuit breaker open (pop-out button will appear), merely depress to reset.
•The Incubator has adjustable leveling legs to compensate for uneven laboratory surfaces.

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3.0 Models & Features
3.1 Dimensions (see also Specification Drawing BCD-18002)
Overall Dimensions - inches (mm): All Models
Height: Exterior: 37.750 inches (958 mm)
Width: 25.625 inches (680 mm)
Depth: 27 inches (685 mm)
Footprint: 22 inches (557 mm) wide x 17.5 inches (445 mm) deep
Height: Interior: 24 inches (611mm)
Width: 20.375 inches (518mm)
Depth: 20.625 inches (524mm)
Shelf Capacity:
Size: 18 inches (457mm) wide x 18.75 inches (476mm) deep
Supplied: 4 Shelves
Maximum Capacity: 16 Shelves
Maximum Weight Capacity 25 lbs. per Water Jacket shelf
125 lbs. per Water Jacket Incubator
(Make sure shelf is empty before changing position or removing.)
Water Pan:
Dimensions: Mean Length 12.0” (305mm)
Mean Width 10.0” (254mm)
Depth 1.5” (38mm)
Capacity: Maximum 2.0 Liters
Recommended Fill 1.5 Liters
3.2 Performance Parameters:
Temperature Control: Performance Determined with O₂ Set Point @ 21.0%
•Temperature Range: 5C to 55C (37°C default) (5C above ambient to 30C maximum ambient)
•Temperature Uniformity: +0.2°C @ 37°C.
•Temperature Accuracy: +0.1°C.
•Temperature Recovery: 0.12°C/minute Average
•Temperature Display Resolution: 0.1°C
•Temperature Sensor Type: Precision Integrated Circuit
•Door: Proportional base duty cycle based on Temperature set point and -20% to
+20% manually adjustable to adapt to ambient conditions.
CO2Control: Available on All Models Performance Determined with O₂ Set Point @ 21.0%
•CO2Range: 0.1 to 20% (default 5%). (0.0 set point idles system)
•CO2Accuracy: +0.1%
•CO2Recovery: Up to 5% +0.2/-0.5 in 5 minutes average.
•CO2Display Resolution: 0.1%
•CO2Control Logic: Fixed Algorithm/Manual Environmental Adaptable.
•CO2Sensor Type: Infrared single source dual wavelength
•Sensor Bay Temp Chamber Set Point +3.5 Degree C.
O2control and monitoring system w/ the Fujikura Zirconia sensor Available on the NU-8631 models
•O2measuring range: 0 to 25 %
•Default Set-Point: 21% (system is idle at this point)
•O2set- point Range: .5 to 21%
•O2Accuracy: +.25%
•O2Recovery 5% +2%: 15 minutes on average
•Connectors on rear panel are provided for Dry contact remote alarm, RS-485 2way communication, and 4-20 mA

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analog output signals for system performance.
Notes: Performance ratings apply ONLY when the Incubator is installed and set up properly per the instructions
in the manual, has stabilized at all set points and is in a stable environment. See sections 8 and 9 for
proper set up.
Idle system will not inject gas or RH and will not alarm.
3.3 Standard Items Packed with Unit
•Four (4) stainless steel shelves
•Eight (8) stainless steel shelf brackets
•One (1) water pan
•Gas supply tube(s)
•Access port plug with breather hole
•One (1), 2 meters (6.5 ft.) electrical cord 10 Amp 18awg *
•Operation and Maintenance Manual
•Operating Instructions
*Do not replace electrical cord with an inadequately rated cord.
A factory supplied replacement is recommended.
3.4 Consult factory for additional Accessories (Ordered Separately)

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4.0 Test Performance & Procedures
All equipment is thoroughly inspected at the NuAire Factory at the time of shipment. Quality control is maintained by
constant surveillance over the product, beginning at the receipt of purchased material and concluding with a final inspection,
which certifies the Incubator performance to the specifications. In all instances where product quality cannot be easily
assessed on the end item, the product is inspected during sub-assembly fabrication. The following test procedures are
conducted on every Water Jacket Incubator and a copy of the test report is included with each Incubator.
4.1 Visual Inspection
•Each Incubator is visually inspected to ensure that the interior is clean and free from scratches, nicks, and burrs
and that all welds, both interior and exterior are finished.
•Painted surfaces are inspected to be free of scratches, nicks, insufficient covering, and noticeable paint runs.
•The doors open and close freely without binding of the hinges.
•The gasket seals the inner glass door to the Incubator tightly across its entire opening.
•The glass door is free of scratches.
4.2 Electrical Tests
•Electrical Leakage Test
All Incubators are required to have a primary-circuit filtering to meet EMC (electromagnetic compatibility)
regulations. Electrical leakage does not exceed 1.0 milliamperes.
•Dielectric Voltage –Withstand Test
1200 VDC (115-volt units) or 1970 VDC (230-volt units) is applied between dead metal parts and the hot/neutral
power source lead with no electrical breakdown using an Associated Research Model 7564SA Quadchek II, 8106
Omnia 6, or 8006 Omnia 6, or other models.
•Grounding Continuity
The resistance between the green bonding conductor of the supply cord and any dead metal part of the cabinet
shall not exceed 0.10 ohms.
4.3 Functional Tests
These functional tests are performed on every Incubator over a minimum of a 48-hour /burn-in period. The results are
recorded on the final Inspection report and a copy is included with each incubator that is shipped. All failures are noted,
corrected, and any failed test is successfully repeated. Changes from any default value of the optional configuration
parameters meant to improve performance are recorded on this report for your reference.
•Control Systems
All performance and diagnostic functions are exercised to insure proper operation of control systems,
components, and alarms.
•Temperature
Each Incubator is monitored for stable temperature control over the 48-hour burn-in period at (37°C).
•CO2Control
Each Incubator is calibrated to function at a 5% CO2level.
The concentration is checked with an independent calibrated instrument.
Each unit is monitored during the 48-hour burn-in period and only accepted with zero failures.
•CO2Recovery
Each Incubator is exercised for CO2recovery time at the end of the 48-hour burn-in period.
The door is opened for 1 minute to deplete the CO2.
After the door is closed, the unit shall recover to 5% +0.2/- 0.5% within a 5-minute period.
•Humidity
Each unit has a default RH level intended to avoid condensation.
The door duty cycles is determined by a Base duty cycle calculation based on the chamber Temperature set point.
Automatically adjusted in response to environmental conditions but can be manually adjusted if needed to control
condensation and tune in the chamber temperature uniformity for the environment that the incubator is installed
in. If unit is master reset these settings will be changed to a default value of 0.0.
The factory default door and setting is: 0.0.

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5Warranty
Details regarding product warranties can be found in the published warranty data separate from this manual and included
within the data packet sent with the unit.
6Shipments
NuAire, Inc. takes every reasonable precaution to assure that your Incubator arrives without damage. Motor carriers are
carefully selected, and shipping cartons have been specifically designed to insure your purchase arrives safely and
undamaged. However, damage can occur in any shipment and the following paragraph outlines those steps you should take
on receipt of a NuAire Incubator to be sure that if damage has occurred, the proper claims and actions are taken immediately.
6.1 Damaged Shipments
6.1.1 Terms are F.O.B. factory, unless stated otherwise.
Therefore, it is important to check each shipment before acceptance.
6.1.2 If there is visible damage, the material can be accepted after the driver makes a notation on the consignee's copy of
the freight bill. Then an inspection must be made to verify the claim against the carrier. This inspection is the basis
of your filing the claim against the carrier.
6.1.3 If concealed damage is found, it is absolutely necessary to NOTIFY THE FREIGHT AGENT AT ONCE and request an
inspection. Without this inspection, the transportation company may not accept a claim for loss or damage. If the
carrier will not perform the inspection, an affidavit must be prepared to state that he was contacted on a certain
date and that he failed to comply with the request. This, along with other papers in the customer's possession will
support the claim.
7 Installation
The Incubator is fastened to the base skid, and it is usually the best procedure to leave the skid attached until the Incubator is
located in its approximate position to facilitate ease in handling. The base skid can then be removed by removing the two
bolts holding the cabinet to the skid. See BCD-18002 for dimensions and features of the incubator.
It is recommended that no less than two people
are present when the incubator needs to be moved.
Examine the Incubator carefully.
INSPECT both the exterior and the interior for any transit damage before discarding the shipping crate.
7.1 Location
In locating the Incubator, consider all possible conditions that might affect its performance as well as laboratory procedures
for its intended purpose. Do not locate near heating or cooling ducts, or next to equipment that generates heat (steam
radiators, stoves, ovens, autoclaves, etc.). Avoid direct sunrays and rapidly moving air currents. These conditions adversely
affect the even heat dissipation required from the exterior surfaces of the Incubator and may cause the temperature
variation in the chamber to exceed specifications as stated in section 3.2. Since the Incubator needs even heat dissipation
on all surfaces in order to maintain an internal temperature variation of less than ± 0.2°C, a minimum of 2 inches (50mm)
must be allowed between the rear and sides of the Incubator and any walls, partitions or obstructions to facilitate adequate
convection of air around the Incubator. Confirm clearance with a tape measure if needed. Adjust the Incubator location
accordingly. In addition, the Incubator location should address access to its power cord for connection/disconnection if
necessary. For maintenance and service purposes, the control center electronics should remain accessible. Leave at least 3
ft of clearance in front for the swinging door.
7.2 Leveling
Prior to use, the incubator should be leveled using a bubble level on a middle shelf in the chamber. The Incubator should
have all 4 leveling feet firmly on the bench or floor. Level from side to side and front to back. By turning the adjustable
leveling feet counterclockwise, raises the Incubator. The leveling feet height should allow for a 2 inches (50mm) space
between Incubator base and the floor. There is a 1-inch-long Stainless-Steel spacer supplied with each foot. Be sure that it
is in place when you are leveling the incubator.
CAUTION
!

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7.3 Interior Chamber, Shelf & Water Pan Preparation
Shelves
Incubator is shipped with the shelves installed. The shelf-mounted water pan is also in place that option is ordered NuAire
recommends decontamination of all surfaces within the interior chamber, glass door, and outer door with gasket. They can
be wiped down with a disinfectant of 70 percent alcohol or a similar non-corrosive antimicrobial agent. Use only
disinfectants that are compatible with the vinyl gasket, the powder coat paint on the outer door and the 304L stainless steel
in the chamber. See BCD-19905
Note: Absolutely no chlorinated or halogen materials are to be used in the chamber.
NOTE: NuAire does not offer any product warranty with respect to cleaning material compatibility. USE AT YOUR
OWN RISK! The information provided above is from raw material suppliers and known general source
documents for use to develop application cleaning SOP’s.
Four (4) shelves are installed in each Incubator. The shelf positions are easily changed by removing the shelf and moving
the horizontal guides to the desired position. If the shelf does not bind or disengage from the horizontal bracket it is
installed correctly. Additional shelves and shelf guides are available.
Water Pan
Place water pan in the center on the bottom of the chamber and fill with SINGLE DISTILLED WATER no purer than 1 Mega
Ohm.
It is recommended to fill the pan to a maximum of about 1/2 inch below the top rim. See Sections 3.1 and 8.2 for more
specifications and operational details regarding proper maintenance instruction regarding the water.

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7.4 Electrical
The electrical supply circuit to the Incubator must conform to all national and local electrical codes.
Consult the serial-data plate, located at the front of the right side of the Incubator, for voltage, cycle, phase, and ampere
requirements before making a connection. Plug the power cord securely into a grounded power source. VOLTAGE SHOULD
NOT VARY MORE THAN 10% FROM SERIAL PLATE RATINGS. Have a qualified technician check with the power source with a
properly rated voltmeter if needed. A separate branch circuit is recommended to prevent possible loss of product due to
overloading or failure of other equipment on the same circuit. A SURGE PROTECTOR IS STRONGLY RECOMMENDED to
avoid power-related faults.
7.5 Precaution for Incubator Filling
To prepare the Incubator for filling, turn on main power switch located on the back panel. The “Maintenance Required”
notification will show on the main screen indicating that the Jacket requires water.
CAUTION:Only turn the Incubator on when you are ready to fill the water jacket.
7.6 Filling & Draining the Water-Jacket
The fill port plug is located on the front top left side behind the door. Install the fill port fitting. Place the tubing over the
adapter and connect the other end to either a funnel or serrated tap. Use single distilled water, NO PURER THAN
1 MEGA OHM. Fill the Water Jacket until the "Maintenance Required" indicator in the display screen turns off. Add an
additional 3 to 4 liters of water, remove the tube adapter, and replace with the fill port plug.
CAUTION
Be sure to position and level the Incubator as desired before filling with water.
DO NOT OVERFILL!
The Water Jacket requires no anti-bacterial agents. The Incubator already incorporates a copper tube producing copper
sulfate which eliminates bacterial growth within the water jacket. ABSOLUTELY NO CHLORINATED OR HALOGEN
MATERIALS ARE TO BE USED IN THE WATER JACKET.
A safety overfill port is located next to the fill port plug so if overfill does occur, the water will be relieved through the port.
This can be drained from the drain plug located on the bottom front under the valance. The drain uses a ball valve to
control the drain water. If the valve stem is perpendicular to the port in the valve it is closed. Turn it clockwise to open. A
white safety plug must be removed prior to draining located on the bottom of the ball valve. It also insures no leakage will
occur.

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7.7 Air Inlet Connection
The air inlet and filter are installed at the Factory. The filter has flexible hose that attaches to bulkhead fittings in the
bottom of the front panel and can be found under the valance that is beneath the door. One 50mm polypropylene .3-
micron HEPA filter is connected to the hoses. The filter is labeled “Air filter”. To remove the valance the outer door must
be open. Reach in under the valance and lift it to remove. See BCD-18476. The air inlet on the back of the incubator is
connected to the filter by a hose at the factory and does not require installation.
CAUTION: This is a free air supply. DO NOT connect to a pressurized air source.
7.8 CO2 / N2Gas Supply Setup
7.8.1 Gas and Air supply HEPA filtering is installed at the
Factory on the front of the incubator as illustrated
in the picture.
Each incubator is equipped with the filters required
for the control systems available on that model.
•CO2/N2Pressure to the In-VitroCell is rated at 20 PSIG or 1.4 BAR.
Do not exceed 25 PSIG or 1.8 BAR to avoid damaging the incubator.
•CO2/N2of medical grade is recommended.
•A two-stage pressure regulator, Linde #19590 (NU-1564)
or equal is recommended.
•DO NOT USE a single stage regulator.
7.8.2 CO2/ N2Gas Supply Tubing Connections
Included with this unit and for all models of the 5800 series is (1) six-foot (2 m) of vinyl tube.
It connects between the low-pressure side of the 2-stage regulator at the CO2 supply and incubator fitting labeled
CO2INLET (as shown in the following Figure). Next, secure each connection with the clamps supplied.
On Model NU-8631 there is a second six foot (2 m) of vinyl tube. It connects between the low-pressure side of the 2
stage regulator at the N2supply gas and incubator fitting labeled N2INLET. Next, secure each connection with the
clamps supplied.
Figure illustrating CO2and N2 gas supply connections
CAUTION
!

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7.8.3 CO2/ N2Supply
•CO2/N2of medical grade is recommended.
•A two-stage pressure regulator, Linde # 19590, or equal, is recommended.
•DO NOT USE a single stage regulator.
It will not give a stable output at 20 psi and exposes the Incubator to the gas cylinder pressure.
•High concentrations of CO2gas can cause asphyxiation!
•Install Incubator in a well-ventilated area.
•Gas Supply pressure to the In-VitroCell is rated at 20psi (1.4 bar).
•Do not exceed 25psi (1.8 bar) or damage to the incubator may occur.
This Incubator is designed to be operated with CO2gas only.
Connecting a flammable or toxic gas can result in a hazardous condition.
Gases other than CO2/N2should not be connected to this equipment.
CO2 gas cylinders have a UN1013 label on the cylinder and are equipped with a CGA 320 outlet valve.
Check the gas cylinder for the proper identification labels.
Do not use CO2gas cylinders equipped with siphon tubes.
A siphon tube is used to extract liquid CO2 from the cylinder which can damage the pressure regulator.
Consult with your gas supplier to ensure that the CO2cylinder does not contain a siphon tube.
7.8.4 CO2/ N2Pressure Regulators
•The regulator’s high-pressure stage direct from the supply cylinder must have a range of from 0 to 2000 PSI or
0 to 140 BAR. This gauge indicates actual tank pressure.
•The low-pressure stage should have a range of 0 to 30 PSI or 0 to 2 BAR (100 PSI or 6 BARS maximum). This
gauge will indicate the actual CO2/N2pressure to the Incubator.
•Some single stage CO2/N2pressure regulators have two gauges. USE A TWO-STAGE REGULATOR.
All NuAire Incubators use CO2/N2in such quantities that precise metering of CO2/N2input pressure is important for
maximum performance.
To connect the regulator:
1. Open the CO2cylinder slightly, for an instant (this is termed “cracking the valve.”)
This will blow out dust or dirt that may have collected in the valve outlet.
BE SURE to keep your face away from the valve outlet to protect your eyes from dust or dirt.
2. Make sure the regulator pressure-adjusting screw is released by turning it counterclockwise
until it turns freely.
3. Attach the regulator to the cylinder valve and tighten the connection nut with a wrench.
BE SURE DISC SEAL IS IN PLACE BEFORE MAKING A CONNECTION.
7.8.5 CO2/ N2Supply Adjustment
With the regulator OFF (i.e. fully counterclockwise), open the cylinder valve slowly usually 1 to 2 turns is sufficient.
NEVER STAND IN FRONT OR BEHIND THE REGULATOR WHEN
OPENING THE VALVE. ALWAYS STAND TO ONE SIDE.
The cylinder tank pressure should read: 700 to 800 PSI (48 to 55 BAR) for CO2 or 2550 to 2650 PSI (176 to 183 BAR)
for N2more or less depending on the temperature of the cylinder.
Next turn each regulator's pressure adjusting knob clockwise until the low-pressure gauge reads 20 PSI or 1.4 BAR.
Both gas connections are now complete.
Note: OSHA requires the CO2tanks to be physically restrained (i.e. via chained to wall)
to prevent accidental damage to cylinder.
7.8.6 Checking the Connections
The connections can be checked for leakage by brushing a small amount of soapy water on each one.
Observe to see if any bubbles are blown. If not, the connection is secured properly.
If it is, the cause of the leaking needs to be determined, (i.e. loose clamp or damaged hose) and corrected.
CAUTION
!
CAUTION
!

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7.9 Removing the cable ties used to secure the Air pump for shipping:
7.10 Setting up the access port:
When utilizing the access port do not block or restrict the relief holes in the center of the port plug. Blocking this venting
can cause performance issues for the incubator. Cut slits or new holes in the vented plug to make passage for cables or
other items requiring access to the chamber if needed.
7.11 Correct Installation
When the Incubator is installed correctly connected to the power source it is rated for gas connections made properly,
the water pan filled, the shelves in place, and the unit is leveled. Read section 8 and follow all instructions for setting set
points at the desired value. Then calibrate the Incubator control systems as explained in section 9. If the Incubator is
installed and calibrated correctly it will meet the performance specs listed in section 3.2.
7.12 Reversing Incubator Hinges
Reversing the incubator hinges from the right to the left side SEE PTB0236 for instructions.
This requires disassembly of the incubator and should be done by a qualified service technician.
8.0 Water Jacket Operation
Safety alert symbol indicates potentially hazardous
Situation which, if not avoided, may result in minor
Or Moderate injury.
CAUTION: A qualified technician who is familiar with the proper maintenance procedures required for this equipment,
as well as repair, must perform all maintenance actions on this equipment.
The Incubator is designed to provide a sterile, constant temperature, constant CO2level and naturally humidified atmosphere
for optimum growth of tissue cell cultures and other organisms requiring this precise environment. To operate the Incubator
properly, the following parameters must be reviewed, carefully set, and/or prepared.
8.1 Sterility
The chamber environment is not selective. The growth environment is applied equally to all microorganisms (specimens
and contaminates) within the chamber. Therefore, before placing any cultures in the Incubator, the shelves and sidewall
top plenums should be sterilized. The interior sidewalls, top, bottom, door, as well as the gasket should be wiped clean
564
191
ATTENTION ACCOMPANY'S
INFORMATION OR IMPORTANT
SYMBOL
POTENTIAL ELECTRICAL
HAZARD ONLY QUALIFIED
PERSON TO ACCESS
188
HOT SURFACE
BURN POTENTIAL
!
CAUTION
!

OM0253 Page 18 of 52
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with a 70% solution of isopropyl alcohol or other disinfectant compatible with the chamber construction, to remove any
contamination. Use mild detergent to clean the exterior of the Incubator.
NOTE: NuAire does not offer any product warranty with respect to cleaning material compatibility. USE AT YOUR
OWN RISK! The information provided above is from raw material suppliers and known general source documents
for use to develop application cleaning SOP’s.
8.2 Humidity
Water Pan available on all Models
Humidification of the Incubator chamber is achieved through the process of water evaporation (vapor water pressure) from
a stainless-steel water pan placed on the bottom of the chamber. Materials of different thermal resistance (e.g., glass,
plastic) do not offer sufficient thermal recovery and are not recommended for water pan usage. Although some metals
offer better thermal coefficients than stainless steel, dissimilar metals cause electrolysis in the acid atmosphere (carbonic
acid) and should never be used, or placed within the chamber.
Use only distilled or mineral-free water, no purer than 1 megaohm, in the stainless-steel pan. The water should be
changed at least once a week. FLOODING THE BOTTOM OF THE INCUBATOR IS NOT RECOMMENDED since it is difficult to
change the water weekly and almost always necessitates the use of chemicals, which are not recommended and may
damage the stainless steel. Also, it promotes condensation on the inner walls because it steals the natural convection, heat
flow through the inner chamber and condensation points occur.
NOTE: ABSOLUTELY NO CHLORINATED OR HALOGEN MATERIALS ARE TO BE USED IN THE CHAMBER.
Humidity Recovery: Using the water pan supplied with the incubator and with a 5.0% CO₂ set point.
NU-8631: Recovery after a door opening is directly dependent on the O₂ set point and ambient RH.
The lower each of these factors is the longer the recovery time.
Contamination in the water pan may be avoided by adding a small amount of copper sulfate to the water pan after each
Incubator decontamination.
NOTE: Please contact the copper sulfate manufacturer to determine the proper amount of copper sulfate
that should be added to the water pan.
Condensation on the glass door / front chamber or in the back of the chamber indicates that the door heater cycle requires
calibration (see section 9.2). The fresh airflow that the air pump delivers to the chamber has been preset at the factory. If
condensation starts forming on the sides or back wall in the chamber, the number and length of air injections into the chamber can
be increased. (See section 8.8.1.2 for instructions.) To alleviate the condensation, increase the airflow. To increase RH in the
chamber reduce the airflow. If airflow is decreased, some condensation may be unavoidable. The port plug with a breather hole
must be used on the side port.
NOTE: DO NOT SEAL OR RESTRICT THE OPENING IN THE PORT PLUG.
THIS IS A PRESSURE RELIEF FOR THE CHAMBER.

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8.3 Control System Introduction
The NuAire Incubator Control Electronics system is designed to serve the control requirements of the Incubator chamber.
Temperature, CO2levels in all models and O2 in available models are controlled by preset values to provide the optimum
conditions for culture growth within a chamber. Operator input is coordinated through the NuTouch LCD keypad and status
displays.
8.3.1 Chamber control in a single electronic package.
8.3.2 Enhanced information presentation on a NuTouch LCD
Main Screen Continuously displays:
•Measured Chamber Temperature
•Measured CO2%
•Measured O2% in available models
•Current Screen ID “MAIN SCREEN”
•A mode status indicator (RUN, STANDBY or Power loss Recovery)
•Door ajar status displays when door is ajar
•Door Closed status displays when door is closed
and flashes during the post door close delay time.
•NuAire Logo button Leads to the System settings
menu when pressed
•Date and Time
•Air, CO2 & O2inject indicators show when gas or RH is injecting.
•PIDT: CO2 & O2indicate that the system showing is in Post Inject Delay Time.
•An Alarm Status Menu
•Maintenance Required displayed
Figure 1 shows the various inputs and outputs of the system.

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8.3.3 Active buttons on the MAIN SCREEN:
Button Description
Action when touched
Control system description
(i.e. TEMP or CO2)
Brings a pop-up screen that describes the control system
that was touched
Measured value of
each control system
Goes to the Environmental Set Point Screen
(Keypad to enter the password will pop up if
the password function is enabled)
NuAire Logo
Goes to “System Settings” Screen for
access to more menus
Date & time display
Goes to screen for adjusting time and date
8.3.4 Accessing the Incubator Control Screens
Accessing the many Control screens, performing functions and diagnostic tests, and maneuvering through the
NuTouch LCD menu system is accomplished through the following methods:
•Touching a “graphic” Icon like the NuAire Logo in the Main Screen to access the System Settings menu. This
is preceded by an Enter Password screen when a password has been established.
•Touching a “Button function” button command that is visible on the screen.
The button will have text in it indication the function you are about to perform.
The following buttons are used on all models:
An explanation of these buttons is as follows:
Save –This command saves the changes you have made and returns you to the calling screen or, in some
cases, back to the main menu.
Exit –This command exits you from the current screen and returns you to the calling screen.
Close –The command causes the current screen to be removed and returns to the calling screen.
Cancel –This command will cancel the changes you have made, and in some circumstances, cancels the
“set of requests” that you have entered and returns you back to the calling screen.
(e.g., cancel in the middle of the Decon screen setup returns you to the calling screen)
Start –This command causes a main event to begin (e.g. Decon Cycle).
Next –This command causes another step (screen), in a list of steps (screens) to be displayed
(e.g., Decon cycles and some CO2calibration screens).
Back –This command returns you to the previous screen.
Decon –This command begins the Decon sequence.
•Touching a “Text” button command. The text or numerical value will be in blue indicating that is a link
similar to the computer or on the internet. The system settings menu screen shown below is a good
example of both the Text commands and Button commands:
NOTE: The Main Screen is the one exception for the above-described buttons.
The green clock text is and is a link to adjusting the time.
The large white Control system measured values are also a “second” link to the
“Environmental Set-Points” menu and the control system descriptions are a link to system
description pop up screens when touched.
8.3.5 Functioning accessed from the System Settings screen:
The “System Settings” menu screen can be accessed from the “Main Screen” by touching the NUAIRE logo:
Environmental Set-Points: Allows adjustment of Temperature, CO2, and Door heater power level settings.
NOTE: You can also access the Environmental set points by touching any
control system numerical value in the MAIN SCREEN.
Performance Monitor: Views a function’s Set-Point and Measured values over the last seven days.
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