Optika B-510 Series User manual

Ver. 3.2 2022
INSTRUCTION MANUAL
B-510 Series
Model
B-510BF
B-510ERGO
B-510ASB
B-510PH
B-510FL
B-510LD4
B-510LD4-SA
B-510LD4D
B-510-2
B-510-2F
B-510-3
B-510-5

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Table of contents
1. Warning 4
2. Safety Information 4
3. Package content 5
3.1 B-510BF/B-510ERGO 5
3.2 B-510PH 6
3.3 B-510ASB 7
3.4 B-510-2/B-510-2F/B-510-3/B-510-5 8
3.5 B-510FL 9
3.6 B-510LD4/B-510LD4-SA 10
3.7 B-510LD4D 11
4. Unpacking 12
5. Intended use 12
6. Symbols and conventions 12
7. Instrument description 13
7.1 B-510BF/B-510ERGO 13
7.2 B-510PH 15
7.3 B-510ASB 17
7.4 B-510-2/B-510-2F/B-510-3/B-510-5 18
7.5 B-510FL 19
7.6 B-510LD4/B-510LD4-SA 20
7.7 B-510LD4D 21
8. Assembling 23
8.1 B-510BF/ERGO/PH/ASB 23
8.2 B-510-2/B-510-2F/B-510-3/B-510-5 24
8.3 B-510FL 27
8.4 B-510LD4/B-510LD4-SA 29
8.5 B-510LD4D 30
8.6 Installing the PC screen (B-510LD4D) 30
8.7 Cable connection (B-510LD4D) 30
8.8 Installing uorescence lter (B-510FL) 32
8.9 Installing uorescence lter (B-510LD series) 35
8.10 Replacing uorescence lter (B-510LD series) 36
8.11 Polarizing set (optional) 37
9. Brightfield observation procedures (B-510BF/B-510ERGO) 38
10. Use of the microscope (B-510BF/B-510ERGO/B-510-2-2F-3-5) 39
10.1 Light intensity adjustment 39
10.2 Coarse focus tension adjustment 39
10.3 Coarse upper limit lever 39
10.4 Stage 40
10.5 Diopter adjustment 40
10.6 Adjusting the interpupillary distance 40
10.7 Light path selection (only uorescence models) 40
10.8 Use of eye shields 41
10.9 Centering the condenser 41
10.10 Effects of the eld diaphragm 41
10.11 Aperture diaphragm 42
10.12 Use of oil immersion objective 42
10.13 Use of the pointer (B-510-2/2F/3/5) 43
10.14 Use of the polarizer (optional) 43
11. Condenser for Brightfield/Darkfield/Phase Contrast (B-510PH) 44
11.1 Brighteld Observation (BF) 44
11.2 Darkeld Observation (DF) 44
11.3 Phase Contrast Observation (PH) 45
11.4 Use of the green lter 46
12. Condenser for Brightfield/Phase Contrast (B-510ASB) 46
12.1 Brighteld Observation (BF) 47
12.2 Phase Contrast Observation (PH) 47
13. Fluorescence observation procedures (B-510FL) 48
14. Fluorescence observation procedures (B-510LD series) 48
15. Use of the microscope (B-510FL/B-510LD4/B-510LD4-SA/B-510LD4D) 49
15.1 Microscope setting (B-510FL) 49
15.2 Use of the microscope (B-510FL) 51
15.2.1 Use of the shutter (B-510FL only) 51

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15.3 Use of the microscope (B-510LD series) 52
15.4 Use of light excluding plate 53
15.5 Use of UV shield 53
16. Simultaneous observation in Phase Contrast + Fluorescence 54
16.1 B-510FL 54
16.2 B-510LD series 54
17. Microphotography 55
17.1 Use of C-mount cameras 55
17.2 Use of reex cameras 55
18. Maintenance 56
19. Troubleshooting 57
Equipment disposal 59

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1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It
is built to high optical and mechanical standards and to withstand daily use. We remind you that this manual contains
important information on safety and maintenance, and that it must therefore be made accessible to the instrument users.
We decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.
2. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the operation voltage
of the equipment and that the lamp switch is in off position. Users should observe all safety regulations of the region. The
equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please
follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.

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① Microscopeframe
② Eyepieces
③ Objectives
④ Observationhead
⑤ Immersionoil
⑥ Allenwrench
⑦ Tensionadjustmenttool
⑧ Dustcover
⑨ Powersupply
③
⑤
⑥
⑦
⑧
⑨
①
②
④
3. Package content
3.1 B-510BF/B-510ERGO

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① Microscopeframe
② Eyepieces
③ Objectives
④ Observationhead
⑤ Centeringtelescope
⑥ Immersionoil
⑦ Allenwrench
⑧ Tensionadjustmenttool
⑨ Dustcover
⑩ Greenlter
⑪ Powersupply
①
②
③④
⑤
⑥
⑦
⑧
⑨
⑩
⑪
3.2 B-510PH

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① Microscopeframe
② Eyepieces
• 10x(onepair)
• 12,5x(onepair)
③ Objectives
④ Observationhead
⑤ Centeringtelescope
⑥ Immersionoil
⑦ Allenwrench
⑧ Tensionadjustmenttool
⑨ Dustcover
⑩ Greenlter
⑪ Powersupply
①
②
③④
⑤
⑥
⑦
⑧
⑨
⑩
⑪
3.3 B-510ASB

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①
②
③
④
⑤
⑥
⑦
⑧
⑩
⑨
① Microscopeframe
② Eyepieces
• 10x/22(onepairformainhead)
• 10x/20(onepairforB-510-2/2F)
• 10x/20(twopairsforB-510-3)
• 10x/20(fourpairsforB-510-5)
③ Objectives
④ Mainobservationhead
⑤ Sideobservationheads
• oneforB-510-2/2F
• twoforB-510-3
• fourforB-510-5
⑥ Immersionoil
⑦ Allenwrench
⑧ Tensionadjustmenttool
⑨ Dustcover
⑩ Powersupply
②
3.4 B-510-2/B-510-2F/B-510-3/B-510-5

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⑪
⑫
① Microscopeframe
② Eyepieces
③ Objectives
④ Observationhead
⑤ Lamphousing
⑥ Epi-illuminator
⑦ HBObulbs
⑧ Allenwrench
⑨ Tensionadjustmenttool
⑩ Dustcover
⑪ UVprotectionshield
⑫ Powersupply
⑬ Powercord
⑭ Fluorescencepowersupply
⑮ Lightexcludingplate
⑯ Immersionoil
①
②
③
④
⑤
⑥
⑦
⑧⑨
⑩
⑬
⑭
⑮
⑯
3.5 B-510FL

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① Microscopeframe
② Eyepieces
③ Objectives
• B-510LD4:W-PLANseries
• B-510LD4-SA:W-PLANFseries
④ Observationhead
⑤ Epi-illuminator
⑥ Allenwrench
⑦ Tensionadjustmenttool
⑧ Immersionoil
⑨ Dustcover
⑩ Powersupply
• 6Vfortransmittedlight
• 12Vforuorescence
⑪ Lightexcludingplate
⑫ UVprotectionshield
①
②
③
④
⑤
⑥
⑦
⑧
⑨
⑩
⑪⑫
⑤
3.6 B-510LD4/B-510LD4-SA

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3.7 B-510LD4D
① Microscopeframe
② Eyepieces
③ Objectives
④ Observationhead
⑤ Epi-illuminator
⑥ PCstick
⑦ Allenwrench
⑧ Tensionadjustmenttool
⑨ Immersionoil
⑩ Dustcover
⑪ Powersupply+powercord
⑫ Lightexcludingplate
⑬ Camerawithmountingbracket
⑭ “C”mount
⑮ Wirelessmouse+Wirelesskeyboard
⑯ UVshield
⑰ PCmonitor
⑱ Multipowercable
⑲ HDMIcable
⑳ USBcable
①
⑮
③
④⑤
⑥
⑦⑧⑨
⑩
⑪
⑫
②
⑱
⑬
⑭
⑯
⑰
⑲⑳

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4. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container and lift the
top half of the container. Take some care to avoid that the optical items (objectives and eyepieces) fall out and get dam-
aged. Using both hands (one around the arm and one around the base), lift the microscope from the container and put it
on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other
residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
5. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the subject.
6. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.

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PHOTO/TV PORT
DIOPTER
ADJUSTMENT
RING
NOSEPIECE
FOCUS
STOP
LEVER
OBJECTIVES
APERTURE
DIAPHRAGM
FIELD
DIAPHRAGM
MAIN SWITCH /
BRIGHTNESS ADJUSTMENT
KNOB
OBSERVATION
HEAD
CONDENSER HEIGHT
ADJUSTMENT KNOB
OBSERVATION
ERGO HEAD
7. Instrument description
7.1 B-510BF/B-510ERGO

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EYEPIECES
STAGE
CONDENSER
BINOCULAR
TUBE
X/Y MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
TENSION ADJUSTMENT RING
SLIDE HOLDER
FINE FOCUS
KNOB
COARSE FOCUS
KNOB
B-510BF/B-510ERGO (opposite side)

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PHOTO/TV PORT
DIOPTER
ADJUSTMENT RING
NOSEPIECE
FINE FOCUS
KNOB
COARSE FOCUS
KNOB
OBJECTIVES
APERTURE
DIAPHRAGM
FIELD
DIAPHRAGM
MAIN SWITCH /
BRIGHTNESS
ADJUSTMENT
KNOB
OBSERVATION
HEAD
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
7.2 B-510PH
FOCUS
STOP
LEVER

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EYEPIECES
STAGE
CONDENSER
BINOCULAR
TUBE
X/Y MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
TENSION ADJUSTMENT RING
SLIDE HOLDER
PHASE RINGS
CENTERING
SCREWS
B-510PH (opposite side)

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PHOTO/TV PORT DIOPTER
ADJUSTMENT RING
NOSEPIECE
FINE FOCUS
KNOB
COARSE FOCUS
KNOB
OBJECTIVES
APERTURE
DIAPHRAGM
FIELD
DIAPHRAGM
MAIN SWITCH /
BRIGHTNESS
ADJUSTMENT KNOB
OBSERVATION
HEAD
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
7.3 B-510ASB
FOCUS
STOP
LEVER

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SIDE
OBSERVATION
HEADS
POINTER COLOR
SELECTOR
POINTER BRIGHTNESS
ADJUSTMENT / POINTER
MOVEMENT
SIDE
OBSERVATION
HEADS
7.4 B-510-2/B-510-2F/B-510-3/B-510-5

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LIGHT PATH
SELECTOR
FLUORESCENCE CUBE
SELECTOR
HBO FLUORESCENCE
LAMP HOUSING
FLUORESCENCE
POWER SUPPLY
7.5 B-510FL
The main microscope commands remain unchanged: only the uorescence parts are highlighted.

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FLUORESCENCE
CUBE SELECTOR
LED FLUORESCENCE
HOUSING
TRANSMITTED
LIGHT BRIGHTNESS
ADJUSTMENT
KNOB
7.6 B-510LD4/B-510LD4-SA
The main microscope commands remain unchanged: only the uorescence parts are highlighted.
UV SHIELD
LIGHT PATH
SELECTOR