Optika B-500BSp User manual

Version: 7
Issued: 16, 03, 2017
Model
B-500BSp
B-500TSp
B-500Bpl
B-500Tpl
B-500Bi
B-500Ti
B-500ERGO
B-500iERGO
B-500Bph
B-500Tph
B-500Biph
B-500Tiph
B-500Ti-2
B-500Ti-3
B-500Ti-5
B-500ASB
B-500Ti2F
500 Series
INSTRUCTION MANUAL

Page 2
Table of Contents
Warning
Symbols and conventions
Safety Information
Intended use
List of accessories and spare parts
Overview
Unpacking
Assembling
Using the microscope
Maintenance
Troubleshooting
Equipment disposal

Page 3
Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of mainte-
nance. It is built to high optical and mechanical standards and to withstand daily use. We remind you that this
manual contains important information on safety and maintenance, and that it must therefore be made acces-
sible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses that does
not comply with this manual.
Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in off position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibil-
ity to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
Intended use
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.

Page 4
List of accessories and spare parts
CAT. NO. DESCRIPTION
M-680 Ergo binocular head 30°-60°
M-625 Eyepiece EWF10x/22mm
M-601 Eyepiece WF15x/16mm
M-602 Eyepiece micrometer EWF10x/22mm
M-005 26x76 mm micrometric slide. Range 1 mm, div. 0,01 mm
M-613 Polarising set ( lters only)
M-614 Rotating table for polarising set
M-618 Dark eld condenser for dry objectives
M-616 Complete phase contrast set with plan obj. 10x, 20x, 40x, 100x
M-617 Complete phase contrast set with plan IOS obj. 10x, 20x, 40x, 100x
M-616.1 Single phase contrast set 40x, plan objective
M-617.1 Single phase contrast set 40x, plan IOS objective
M-619 Photo adapter for REFLEX camera with FULL FRAME sensor
M-699 Universal adapter for OPTIKAM & DIGI cameras (only models with eyepiece adapter, 23mm)
M-620 C-Mount adapter for 1/3” sensor.
M-620.1 C-Mount adapter for 1/2” sensor.
M-515 Halogen bulb 12V/20W, with dichroic mirror
M-034 Dust cover type 5
M-975 Blue filter, 45 mm diameter
M-977 Green filter, 45 mm diameter
M-979 Yellow filter, 45 mm diameter
M-989 Frosted glass fifi lter, 45 mm diameter
ST-036 Eyecups (pair) type 2
M-173 Photo adapter for APS-C and Full Frame Reflfl ex cameras
M-114 C-Mount adapter for 1/2” sensor.
M-116 C-Mount adapter for 2/3” sensor.
M-113.1 Ring adapter, 30mm (for monocular and binocular microscopes)
M-778 C-mount adapter for 1/3”,1/2” and 2/3” sensor
M-666.500 Heating stage, with digital temperature controller B-500 series
M-666.500ph Heating stage, with digital temperature controller B-500Ph
15008 OPTIKA immersion oil 10ml

Page 5
Overview
NOSEPIECE
DIOPTRIC
ADJUSTMENT
RING
EYEPIECE
CONDENSER
CENTERING
KNOBS
APERTURE
DIAPHRAGM
FIELD DIAPHRAGM
TRANSLATION KNOB
FINE FOCUSING
KNOB
COARSE
FOCUSING
KNOB
TENSION
ADJUSTEMENT
KNOB
OBJECTIVE
STAGE

Page 6
Put the microscope stand on a
solid table.
Insert the optical head abo-
ve the stand and tighten the
screw. (Fig.1)
Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Assembling
Once you open the box, these are the microscope’s components:
① Condenser
② Optical head
③ Main body
④ Eyepieces
⑤ Eyepiece cups
⑥ Stage
⑦ Objectives
①
②
③
④
⑤
⑥
⑦
Fig.1

Page 7
Mount the stage: lower the
support using the coarse
focus knob, then place the
stage and rmly tighten the
lock screw ①. (Fig.4)
Insert both eyepieces into
the tubes of the optical head.
(Fig.2)
Insert the condenser under
the stage: position until it is
well inserted into its holder
(under the condenser there is
a pin that must fully enter the
guide of the holder). (Fig. 3)
Screw each objective into
the thread of the nosepiece,
in order of magnication.
(Fig.5)
Fig.2
Fig.3
Fig.4
Fig.5

Page 8
Insert the power supply cable
on the connector at the rear.
(Fig.6)
Fig.6

Page 9
In order to activate the transmitted light illumi-
nator, insert the plug of the power supply cable
into the socket and turn on the main switch on
the back of the main body. Turn the brightness
adjustment knob to a brightness suitable for
observation. (Fig.7)
Loosen the lock-screw, turn
the observation head to
a comfortable position for
observation, and then lock the
lock-screw. (Fig.8)
LED settings
Adjust the observation
head
MAIN
SWITCH
Using the microscope
Fig.7
Fig.8
Hold the right and left parts of
the observation head using
both hands and adjust the
interpupillary distance by
turning the two parts until one
circle of light can be seen.
(Fig.9)
Adjust interpupillary
distance
Fig.9
Place the specimen on
the stage
Fix the specimen slide on the
mechanical stage using the
slide-clamp. Ensure that the
specimen is centred over the
stage opening. (Fig.10)
Fig.10

Page 10
Focus tension
adjustment
Turn the tension-adjust knob
to get a suitable tension for
the focus system. (Fig.11)
NOTE: if the tension is too lo-
ose, the stage could go lower
by itself or the focus easily
lost after ne adjustment. In
this case, rotate the knob in
order to increase the friction. Fig.11
Focus-stop knob
Loosen the focus-stop knob,
rotate the coarse focusing
knob to bring the slide into
focus with a 4X or 10X objecti-
ve. Then rotate the focus-stop
in order to block the height of
the stage. This simplies the
next focusing operations.
The focus-stop knob is also
useful to avoid accidental con-
tacts between objective and
specimen. (Fig.12) Fig.12
Diopter adjustment
Adjust the ne focusing knob
to get the image sharp and
clear while observing with
your right eye, then turn the
left diopter ring to a sharp
and clear image also with the
other eye. The highpoint eye-
pieces allow the user to wear
glasses. (Fig.13)
NOTE :For the optimal para-
focality of the image, it’s sug-
gested to wear your glasses
during the normal use of the
microscope. Fig.13