Optika B-383LD1 User manual

Version: 2
Issued: 18, 03, 2015
Model
B-383LD1
B-383LD2
B-380 Series
INSTRUCTION MANUAL

Page 2
Table of Contents
Warning
Symbols and conventions
Safety Information
Intended use
Package Contents
Unpacking
Product specications
Overview
Using the microscope
Using the uorescence
Maintenance
Troubleshooting
User replaceable accessories and spare parts
Equipment disposal

Page 3
Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of mainte-
nance. It is built to high optical and mechanical standards and to withstand daily use.
We remind you that this manual contains important information on safety and maintenance, and that it must
therefore be made accessible to the instrument users.
We decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.
Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the op-
eration voltage of the equipment and that the lamp switch is in off position.
Users should observe all safety regulations of the region. The equipment has acquired the CE safety label.
However, users have full responsibility to use this equipment safely.
Please follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.
Intended use
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
Package Contents
DESCRIPTION QUANTITY
Microscope stand with nosepiece, stage, condenser 1
Optical head (trinocular) 1
Objective E-PLAN IOS 4x 1
Objective E-PLAN IOS 10x 1
Objective E-PLAN IOS 20x 1
Objective E-PLAN IOS 40x 1
Objective PLAN IOS MET 50x 1
Eyepiece WF10x/20mm 2
Fluorescence attachment 1
Dust cover 1
Power supply output 6Vdc 1

Page 4
Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Place the observation head onto the top of the arm and tighten the lock-screw. Insert the eyepieces into the eye
tubes and x them with the tiny screws on the side of the eye tubes. Remove the plastic protection lm from the
microscope stage.
Product specications
B-383LD1
Head: Trinocular, 30° inclined, 360° rotating. Interpupillary adjustment 48-75 mm.
Eyepieces: WF10X/20mm.
Nosepiece: 5 position on reversed revolving nosepiece. Ball bearing rotation.
Objectives: IOS E-PLAN 4x/0.10, 10x/0.25, 20x/0.40, 40x/0.65, and PLAN 50x/0.75 (no cover slide).
Focusing system: Coaxial coarse and fine..
Stage: Double layer mechanical sliding stage, dimensions 216x150mm, moving range 78x54mm.
Belt-drive in X direction.
Condenser: Abbe condenser, sliding-in, N.A. 1.25 with centering system.
Illumination: Transmitted light: X-LED3, with manual brightness control.
Epi-fluorescence: high-power blue LED.
Power supply: External power supply: Input 100-240Vac 50-60Hz / Output 6Vdc 1A.
B-383LD2
Head: Trinocular, 30° inclined, 360° rotating. Interpupillary adjustment 48-75 mm.
Eyepieces: WF10X/20mm.
Nosepiece: 5-position reversed revolving nosepiece. Ball bearing rotation.
Objectives: IOS E-PLAN 4x/0.10, 10x/0.25, 20x/0.40, 40x/0.65, and PLAN 50x/0.75 (no cover slide).
Focusing system: Coaxial coarse and fine.
Stage: Double layer mechanical sliding stage, dimensions 216x150mm, moving range 78x54mm.
Belt-drive in X direction.
Condenser: Abbe condenser, sliding-in, N.A. 1.25 with centering system.
Illumination: Transmitted light: X-LED3, with manual brightness control.
Epi-fluorescence: high-power white LED (for B ang G filtersets).
Power supply: External power supply: Input 100-240Vac 50-60Hz / Output 6Vdc 1A.

Page 5
Overview
PHOTO/VIDEO PORT
HEAD LOCKING
SCREW
REVOLVING NOSEPIECE
STAGE
TRANSLATION KNOBS
BRIGHTNESS
ADJUSTMENT
(LEFT SIDE)
CONDENSER CENTERING
SCREWS
IRIS DIAPHRAGM
EYEPIECE
CONDENSER
LED ILLUMINATOR
OBJECTIVE
DIOPTRIC
ADJUSTMENT RING

Page 6
SLIDE CLAMP
FINE FOCUSING KNOB
CONDENSER HEIGHT
ADJUSTMENT
TENSION
ADJUSTMENT KNOB
COARSE FOCUSING KNOB
FOCUS STOP KNOB
INTERPUPILLARY DISTANCE

Page 7
Using the microscope
Adjust the observation head
Loosen the lock-screw, turn the observation head to a comfortable position for observation, and then lock the
lock-screw.
Place the specimen on the stage
Lock the specimen slide on the mechanical stage using the slide clamp. Ensure that the specimen is centred
over the stage opening by adjusting the coaxial knobs of the stage
LED lamp settings – Transmitted illumination
The microscope is tted with high brightness white LED illuminator. Press the selector switch on the back of the
main body to I position. Turn the brightness adjustment knob to a brightness suitable for observation.
LED lamp settings – Epillumination
The microscope is tted with high brightness LED illuminator. Press the selector switch on the back of the main
body to II position. Turn the brightness adjustment knob to a brightness suitable for observation.
Adjust interpupillary distance
Hold the right and left parts of the observation head by both hands and adjust the interpupillary distance by turn-
ing the two parts until one circle of light can be seen.
Focus and diopter adjustment
Turn the dioptric adjustment ring on the left eyepiece to the zero position. Turn the coarse focus knob in order
to focus the slide with an objective with low magnication. Adjust the ne focus knob until you obtain a clear and
dened picture observing with the right eye, and then act on the left dioptric compensation ring observing with
the left eye. When the image appears in focus, choose the necessary objective with the revolving nosepiece.
Turn the tension-adjust-knob to get a suitable tension for the focus system.
Condenser adjustment
Turn the condenser adjustment knob to move the condenser up or down for a clear and uniform sample illumina-
tion. The two condenser centring screws can be used to centre the condenser.
Set the numerical aperture
Adjust the aperture of the iris diaphragm to set the numerical aperture of the illuminator, thus controlling image
contrast and resolution.

Page 8
HEAD LOCK SCREW
LED ILLUMINATOR
ILLUMINATOR LOCK
SCREW
FILTER SELECTOR (1)

Page 9
JACK FROM THE
POWER SUPPLY
(LOWER POSITION)
JACK FROM THE
ILLUMINATOR
(UPPER POSITION)
MAIN SWITCH
SLIDE THE BLACK
PLATE BETWEEN
STAGE AND CLAMP
Using the uorescence
Assembling the epi-uorescence attachment
Take the attachment out of its packaging and put it onto the microscope stand, then tighten the attachment lock-
ing screw.
Place the optical head onto the top of the attachment and tighten the optical head locking screw. Connect the
cable from the epi-uorescence attachment to the output jack on the rear of the microscope.
Lighting up the uorescence LED light
Connect the external 6Vdc power supply to a wall socket, and plug its output to the jack on the rear of the mi-
croscope:
In order to light up the epi-uorescence LED, press the main switch on the rear in position II. Then rotate the
brightness adjustment knob on the left to the desired value. Pull the lter selector (1) to right or left in order to
insert the uorescence lter into the optical path. Put the lter selector (1) in the center if you want to use the
brighteld transmitted illumination. Unlike a mercury lamp system, B-383LD LED illumination doesn’t need any
power-up time for heating, and can be used immediately after switching on. Also, the LED source is pre-aligned
in factory and doesn’t need any alignment operation.
Looking at the uorescent specimen
Focus on your sample, and adjust the light intensity as needed through the brightness adjustment knob. In order
to improve the darkness of the background (thus improving contrast), it is strongly suggested to put the provided
black plate under the stage):

Page 10
Maintenance
Microscopy environment
This microscope is recommended to be used in a clean, dry and shock free environment with a temperature of
0-40°C and a maximum relative humidity of 85 % (non condensing). Use a dehumidier if needed.
To think about when and after using the microscope
• The microscope should always be kept vertically when moving it and be careful so that no
moving parts, such as the eyepieces, fall out.
• Never mishandle or impose unnecessary force on the microscope.
• Never attempt to service the microscope yourself.
• After use, turn off the light immediately, cover the microscope with the included
dust-cover, and keep it in a dry and clean place.
Electrical safety precautions
• Before plugging in the power supply, make sure that the supplying voltage of your region
matches with the operation voltage of the equipment and that the lamp switch is in off-
position.
• Users should observe all safety regulations of the region. The equipment has acquired
the CE safety label. However, users do have full responsibility to use this equipment safely.
Cleaning the optics
• If the optical parts need to be cleaned try rst to: use compressed air.
• If that is not sufcient: use a soft lint-free piece of cloth with water and a mild detergent.
• And as a nal option: use the piece of cloth moistened with a 3:7 mixture of ethanol and ether.
Note: ethanol and ether are highly ammable liquids. Do not use them near a heat source, near sparks or
near electric equipment. Use these chemicals in a well ventilated room.
• Remember to never wipe the surface of any optical items with your hands. Fingerprints can damage the
optics.
• Do not disassemble objectives or eyepieces in attempt to clean them.
For the best results, use the OPTIKA cleaning kit (see catalogue).
If you need to send the microscope to Optika for maintenance, please use the original packaging.