Optika OPTISTAR OS-A1 Series User manual

Model
OS-A1
OS-A2
OS-A3
OS-B2
OS-B3
OS-C3
OPTISTAR By OPTIKA Series
INSTRUCTION MANUAL
Ver. 1.0 2021

Page 2
1. Warning 3
2. Safety Information 3
3. Unpacking 3
4. Intended use 3
5. Symbols and conventions 3
6. Instrument description 4
6.1 OS-A1 4
6.2 OS-A2 5
6.3 OS-A3 6
6.4 OS-B2 7
6.5 OS-B3 8
6.6 OS-C3 9
7. Assembling 10
7.1 All models 10
7.1.1 OS-A / OS-C Series 10
7.1.2 OS-B Series 10
7.2 OS-B3 10
7.3 OS-C3 10
8.1 Switching on the microscope 11
8.1.1 OS-A1 11
8.1.2 OS-A2 / OS-A3 11
8.1.3 OS-B2 / OS-B3 11
8.1.4 OS-C3 11
8.2 Light intensity adjustment 12
8.2.1 OS-A1 12
8.2.2 OS-A2 / OS-A3 12
8.2.3 OS-B2 / OS-B3 / OS-C3 12
8.3 Coarse focus tension adjustment 12
8.3.1 OS-B2 / OS-B3 12
8.3.2 OS-C3 13
8.4 Stage 13
8.4.1 OS-A1 13
8.4.2 All other models 13
8.5 Adjust the interpupillary distance 13
8.5.1 OS-A3 13
8.5.2 OS-B / OS-C 14
8.6 Diopter adjustment 14
8.6.1 OS-B Series 14
8.6.2 OS-C Series 14
8.7 Use of oil immersion objective 15
8.8 Condenser centering (OS-B Series only) 15
8.9 Aperture diaphragm 15
8.9 Useofcolorlter(OS-BSeriesonly) 16
9. Microphotography 17
10. Maintenance 18
11. Troubleshooting 19
Equipment disposal 20
Table of contents

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It
is built to high optical and mechanical standards and to withstand daily use. We remind you that this manual contains
important information on safety and maintenance, and that it must therefore be made accessible to the instrument users.
We decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.
2. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the operation voltage
of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety regulations of the region. The
equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please
follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.
3. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container and lift the
top half of the container. Take some care to avoid that the optical items (objectives and eyepieces) fall out and get dam-
aged. Using both hands (one around the arm and one around the base), lift the microscope from the container and put it
on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other
residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
5. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.

Page 4
FOCUS
KNOB
UPPER STOP SCREW
STAGE
OBJECTIVES
STAGE CLIPS
OBSERVATION HEAD
EYEPIECE
6. Instrument description
6.1 OS-A1
ON-OFF / INTENSITY
ADJUSTMENT DIAL

Page 5
6.2 OS-A2
EYEPIECE
INCIDENT LIGHT
ON-OFF / INTENSITY
ADJUSTMENT DIAL
STAGE
OBJECTIVES
OBSERVATION HEAD
INCIDENT LIGHT
ILLUMINATOR
SLIDE HOLDER
FINE FOCUS
KNOB
COARSE FOCUS
KNOB
UPPER STOP SCREW
TRANSMITTED LIGHT
ON-OFF / INTENSITY
ADJUSTMENT DIAL
(LEFT SIDE)
FILTER HOLDER

Page 6
EYEPIECES
6.3 OS-A3
INCIDENT LIGHT
ON-OFF / INTENSITY
ADJUSTMENT DIAL
STAGE
OBJECTIVES
OBSERVATION HEAD
INCIDENT LIGHT
ILLUMINATOR
SLIDE HOLDER
FINE FOCUS
KNOB
COARSE FOCUS
KNOB
INTERPUPILLARY
ADJUSTMENT KNOB
UPPER STOP SCREW
FILTER HOLDER
TRANSMITTED LIGHT
ON-OFF / INTENSITY
ADJUSTMENT DIAL
(LEFT SIDE)

Page 7
6.4 OS-B2
FINE FOCUS
KNOB
ON-OFF / INTENSITY
ADJUSTMENT DIAL
(LEFT SIDE)
STAGE
OBJECTIVES
COARSE FOCUS
KNOB
CONDENSER
OBSERVATION HEAD
EYEPIECE
SLIDE HOLDER
X/Y MOVEMENT
KNOBS
TENSION
ADJUSTMENT
RING
DIOPTER
CORRECTION
RING
CONDENSER HEIGHT
ADJUSTMENT KNOB

Page 8
6.5 OS-B3
FINE FOCUS
KNOB
INTENSITY
ADJUSTMENT DIAL
(LEFT SIDE)
STAGE
OBJECTIVES
COARSE FOCUS
KNOB
CONDENSER
OBSERVATION HEAD
EYEPIECE
SLIDE HOLDER
PHOTO TUBE
X/Y MOVEMENT
KNOBS
DIOPTER
CORRECTION
RING
LIGHT PATH
SELECTION LEVER
TENSION
ADJUSTMENT
RING
CONDENSER HEIGHT
ADJUSTMENT KNOB

Page 9
6.6 OS-C3
STAGE
OBSERVATION HEAD
FINE FOCUS
KNOB
MAIN SWITCH
COARSE FOCUS
KNOB
CONDENSER
EYEPIECE
SLIDE HOLDER
X/Y MOVEMENT
KNOBS
TENSION
ADJUSTMENT
RING
PHOTO TUBE
INTENSITY
ADJUSTMENT DIAL
(LEFT SIDE)
OBJECTIVES

Page 10
7. Assembling
7.1 All models
1. Remove the dust protection cap from the eyepiece sleeve
and insert the eyepiece into the empty eyepiece sleeve. (Fig.
1)
FFig. 1ig. 1
FFig. 2ig. 2
FFig. 3ig. 3
7.1.1 OS-A / OS-C Series
Insert the power supply jack in the socket ①placed at the rear
side of the microscope. (Fig. 2)
7.1.2 OS-B Series
Insert the power cord in the socket placed at the rear side of the
microscope.
7.2 OS-B3
1. Remove the dust protection cap of the photo tube.
2. Insert the “Eyepiece Linker” ②. (Fig. 3)
3. Insert the projection lens in the empty hole of the Eyepiece
Linker.
• The camera will not be in focus if the Eyepiece Linker is
not used.
①
②
FFig. 4ig. 4
7.3 OS-C3
1. Unscrew the locking screw ③on the right side of the photo
port and remove the dust protection cap. (Fig. 4)
2. Insert the photo tube ④ in the empty hole and lock the screw.
3. Insert the projection lens in the empty hole of the photo tube.
③
④

Page 11
8. Use of the microscope
8.1 Switching on the microscope
8.1.1 OS-A1
Operate on the main switch ①placed in the rear side of the mi-
croscope, moving the selector on “I”. (Fig. 5)
FFig. 5ig. 5
FFig. 6ig. 6
FFig. 7ig. 7
FFig. 8ig. 8
①
②
8.1.2 OS-A2 / OS-A3
1. Operate on the main switch/light intensity dial ②placed on
the left side of the microscope, to turn on transmitted light.
(Fig. 6)
2. Operate on the main switch/light intensity dial ③placed on
the right side of the microscope, to turn on incident light.
8.1.3 OS-B2 / OS-B3
Operate on the main switch ④placed in the rear side of the mi-
croscope, moving the selector on “I”. (Fig. 7)
③
④
8.1.4 OS-C3
Operate on the main switch ⑤placed on the right side of the
microscope, moving the selector on “I”. (Fig. 8)
⑤

Page 12
8.2 Light intensity adjustment
8.2.1 OS-A1
Operate on the light intensity dial ① to increase or decrease the
illumination intensity. (Fig. 9)
FFig. 9ig. 9
FFig. 10ig. 10
FFig. 11ig. 11
FFig. 12ig. 12
①
②③
④
①
8.2.2 OS-A2 / OS-A3
1. Operate on the main switch/light intensity dial ②placed on
the left side of the microscope, to increase or decrease the
transmitted light illumination intensity. (Fig. 10)
2. Operate on the main switch/light intensity dial ③placed on
the right side of the microscope, to increase or decrease the
incident light illumination intensity.
8.2.3 OS-B2 / OS-B3 / OS-C3
Operate on the light intensity dial ④ to increase or decrease the
illumination intensity (Fig. 11)
8.3 Coarse focus tension adjustment
8.3.1 OS-B2 / OS-B3
The coarse knob tension is preset in the factory.
1. To modify the tension according to personal’s needs, rotate
the ring ①. (Fig. 12)
• Clockwise rotation increases the tension.
• If the tension is too loose, the stage could go lower by itself or
the focus easily lost after ne adjustment. In this case, rotate
the knob in order to increase the tension.

Page 13
8.3.2 OS-C3
The coarse knob tension is preset in the factory.
1. To modify the tension according to personal’s needs, rotate
the ring ①using a flat screwdriver. (Fig. 13)
• Clockwise rotation increases the tension.
• If the tension is too loose, the stage could go lower by itself or
the focus easily lost after ne adjustment. In this case, rotate
the knob in order to increase the tension.
FFig. 13ig. 13
FFig. 14ig. 14
FFig. 15ig. 15
FFig. 16ig. 16
①
②
③
④
8.4 Stage
8.4.1 OS-A1
Put the slide on the stage and hold it using the stage clips ②.
(Fig. 14)
8.4.2 All other models
Stage accepts standard slides 26 x 76 mm, thickness 1,2 mm
with coverslide 0,17 mm. (Fig. 15)
1. Open the spring arm of the slide holder ③ and place the slide
from the front on the stage.
2. Gently release the spring arm of the slide holder.
• A sudden release of the spring arm could cause the fall-
ing of the slide.
8.5 Adjust the interpupillary distance
8.5.1 OS-A3
Rotate toward left or right the interpupillary distance knob ④ until
one circle of light can be seen. (Fig. 16)
The range of the interpupillary distance is 55- 75 mm.

Page 14
FFig. 19ig. 19
③
FFig. 17ig. 17
①
FFig. 18ig. 18
②
8.5.2 OS-B / OS-C
1. Hold the right and left parts of the observation head using
both hands and adjust the interpupillary distance by turning
the two parts until one circle of light can be seen. (Fig. 17)
• The graduation on the interpupillary distance indicator ①,
pointed by the spot “.” on the eyepiece holder, shows the
distance between the operator’s eyes.
The range of the interpupillary distance is 55- 75 mm.
8.6 Diopter adjustment
8.6.1 OS-B Series
1. Look into the left eyepiece with your left eye only, and focus
on the specimen.
2. Look into the right eyepiece with your right eye only. If the im-
age is not sharp, use the diopter adjustment ring ②to com-
pensate. (Fig. 18)
• The adjustment range is ±5 diopter. The number indicat-
ed on the adjustment ring graduation should correspond
to the operator’s diopter correction.
8.6.2 OS-C Series
1. Look into the right eyepiece with your right eye only, and fo-
cus on the specimen.
2. Look into the left eyepiece with your left eye only. If the image
is not sharp, use the diopter adjustment ring ③to compen-
sate. (Fig. 19)
• The adjustment range is ±5 diopter. The number indicat-
ed on the adjustment ring graduation should correspond
to the operator’s diopter correction.

Page 15
8.7 Use of oil immersion objective
• OS-B / OS-C Series
1. Focus the specimen with a low power objective.
2. Lower the stage.
3. Put a drop of oil (provided) on the area of the specimen to be
observed. (Fig. 20)
• Make sure that there are no oil bubbles. Air bubbles in
the oil damage the image quality.
• To check for bubbles: remove an eyepiece, fully open the ap-
erture diaphragm and observe the objective exit pupil. (The
pupil must be circular and bright).
4. Insert immersion objective.
5. Return the stage to the upper focusing point and obtain an
optimal focus using the ne focus knob.
• To remove the air bubbles, gently move the nosepiece to the
right and left to move the immersion objective a few times
and allow the air bubbles to move away.
6. After use, gently remove the oil with a soft paper towel or a
lightly moistened optic paper with a mixture of ethyl ether
(70%) and absolute ethyl alcohol (30%).
• The immersion oil, if not immediately cleaned, could
crystallize creating a glass-like layer.
• In this situation the observation of the specimen would
bedi󰀩cult(evennotimpossible)duetothepresenceof
an additional thickness on the objective.
FFig. 20ig. 20
FFig. 21ig. 21
8.8 Condenser centering (OS-B Series only)
1. Focus the specimen with 4x objective.
2. Fully close the aperture diaphragm by rotating the ring ①.
(Fig. 21)
3. If the condenser is not perfectly centered, move the screws
② until the diaphragm image is in the center of the eld of
view.
4. Fully open the diaphragm.
8.9 Aperture diaphragm
• The Numerical Aperture (N.A.) value of the aperture dia-
phragm a󰀨ects the image contrast. Increasing or reducing
this value one can vary resolution, contrast and depth of fo-
cus of the image. Rotate the diaphragm ring toward left or
right until a good contrast is achieved.
• With low contrast specimens set the numerical aperture to
about 70%-80% of the objective’s N.A. If necessary, remove
on eyepiece and, looking into empty sleeve, adjust the con-
denser’s diaphragm in order to obtain an image like the one
in Fig. 22.
①②
②
FFig. 22ig. 22
APERTURE
DIAPHRAGM
FIELD OF VIEW
30-20%
70-80%

Page 16
8.9 Useofcolorlter(OS-BSeriesonly)
Rotate the lter holder ① (Fig. 23) to insert a suitable color lter
for the specimen under observation.
FFig. 23ig. 23
①

Page 17
9. Microphotography
• Refer to specic camera Instruction manual for detailed explanation about microphotography procedure.
• Instruction manual can be downloaded from this link: https://www.optikamicroscopes.com/optikamicroscopes/down-
load/optistar-by-optika-digital-cameras/

Page 18
10. Maintenance
Microscopy environment
This microscope is recommended to be used in a clean, dry and shock free environment with a temperature of 5°-40°C and
a maximum relative humidity of 75 % (non condensing). Use a dehumidier if needed.
To think about when and after using the microscope
• The microscope should always be kept vertically when moving it and be careful so that no moving
parts, such as the eyepieces, fall out.
• Never mishandle or impose unnecessary force on the microscope.
• Never attempt to service the microscope yourself.
• After use, turn o󰀨 the light immediately, cover the microscope with the provided dust-cover, and keep
it in a dry and clean place.
Electrical safety precautions
• Before plugging in the power supply, make sure that the supplying voltage of your region matches
with the operation voltage of the equipment and that the lamp switch is in o󰀨-position.
• Users should observe all safety regulations of the region. The equipment has acquired the CE safety
label. However, users do have full responsibility to use this equipment safely.
Cleaning the optics
• If the optical parts need to be cleaned try rst to: use compressed air.
• If that is not su󰀩cient: use a soft lint-free piece of cloth with water and a mild detergent.
• And as a nal option: use the piece of cloth moistened with a 3:7 mixture of ethanol and ether.
• Note:ethanolandetherarehighlyammableliquids.Donotusethemnearaheatsource,nearsparksornear
electric equipment. Use these chemicals in a well ventilated room.
• Remember to never wipe the surface of any optical items with your hands. Fingerprints can damage the optics.
• Do not disassemble objectives or eyepieces in attempt to clean them.
For the best results, use the dedicated cleaning kit.

Page 19
Review the information in the table below to troubleshoot operating problems.
PROBLEM CAUSE SOLUTION
I. Optical Section:
LED operates, but eld of view remains
dark. Power supply is unplugged. Connect
Brightness is too low Set brightness to a proper level
Dirt or dust is visible in the eld of view. Dirt/dust on the specimen Clean the specimen
Dirt/dust on the eyepieces Clean the eyepieces
Image looks double Aperture diaphragm is stopped down
too far Open aperture diaphragm
Visibility is poor.
• Image is not good.
• Contrast is poor.
• Details are unsharp.
• Image glares
Revolving nosepiece is in an incorrect
position Move the nosepiece to a click stop
Aperture diaphragm is too closed or to
open Adjust aperture diaphragm
Dust or dirt on lenses (condenser, ob-
jectives, eyepieces and slide) Clean thoroughly
For transmitted light observation, the
coverglass thickness must not exceed
0.17mm
Use a coverglass with thickness
0.17mm
Focus is not even Slide holder is not flat. Move the spec-
imen to a flat position
One side of the image is out of focus. The nosepiece is not in the center of
the light path Turn the nosepiece to a click stop
The specimen is out of place (tilted) Place the specimen at on the stage.
The optical performance of the sample
cover glass is poor Use a cover glass of better quality
II. Mechanical Section:
The coarse focus knob is hard to turn. The tension adjustment collar is too
tight Loosen the tension adjustment collar
The focus is unstable. The tension adjustment collar is too
loose Tighten the tension adjustment collar
III. Electric section:
The LED doesn’t turn on. No power supply Check the power cord connection
The brightness is not enough The brightness adjustment is low Adjust the brightness
The light blinks The power cord is poorly connected Check the power cord
IV. Observation tube:
Field of view of one eye does not match
that of the other. Interpupillary distance is incorrect. Adjust interpupillary distance.
Incorrect diopter adjustment.Adjust diopter.
Your view is not accustomed to micro-
scope observation. Upon looking into eyepieces, try look- ing at overall field before concentrating
on specimen range. You may also find
it helpful to look up and into distance
for a moment before looking back into
microscope.
V. Microphotography:
Image edge is unfocused To a certain extent it is due to achro-
matic objectives features To minimize the problem, set the aper- ture diaphragm in a proper position
Bright spots appear on the image Stray light entering in the microscope
through eyepieces or camera viewfind-
er.
Cover eyepieces and viewfinder with a
dark cloth
11. Troubleshooting

Page 20
Equipment disposal
Art.13 Dlsg 25 July 2005 N°151. “According to directives 2002/95/EC, 2002/96/EC and 2003/108/EC relating to the reduc-
tion in the use of hazardous substances in electrical and electronic equipment and waste disposal.”
The basket symbol on equipment or on its box indicates that the product at the end of its useful life should be collected sep-
arately from other waste. The separate collection of this equipment at the end of its lifetime is organized and managed by
the producer. The user will have to contact the manufacturer and follow the rules that he adopted for end-of-life equipment
collection. The collection of the equipment for recycling, treatment and environmentally compatible disposal, helps to pre-
vent possible adverse e󰀨ects on the environment and health and promotes reuse and/or recycling of materials of the equip-
ment. Improper disposal of the product involves the application of administrative penalties as provided by the laws in force.