OPTO-EDU A14.1063 User manual

To ensure the safety and obtain satisfactory performance, please study this
instruction manual thoroughly before start to use your instrument.
A14.1063, A14.1064
Inverted Biological Microscope
A16.1063, A16.1064
Inverted LED Fluorescent Microscope
Instruction Manual

Contents
Before Using ……………………………………………………………………
1
Safety Symbol…………………………………………………………………
1
Safety Precautions ……………………………………………………………
1
Care and Maintenance…………………………………………………………
2
Chapter 1 Introduction …………………………………………………………
4
1.1 Technical Specifications …………………………………………………
4
1.1.1 Main Technical Specifications and Configuration……………………
4
1.1.2 Objective Parameters…………………………………………………
5
1.1.3 Electrical Parameters…………………………………………………
5
1.2 Components ………………………………………………………………
6
Chapter 2 Overview of Agencies ………………………………………………
8
Chapter 3 Regulation and Operation …………………………………………
12
3.1 Turning the Power on ……………………………………………………
12
3.2 Turning the Illumination switch on………………………………………
12
3.2.1 Manual Lighting Switch……………………………………………
12
3.2.2 ECO Switch (Optional functions) …………………………………
12
3.3 Adjusting the Illumination Brightness……………………………………
13
3.4 Specimen Placement and Stage Knob Adjustment………………………
13
3.4.1 Mechanical Stage……………………………………………………
13
3.4.2 Pallet…………………………………………………………………
14
3.4.3 Auxiliary Stage………………………………………………………
14
3.5 Adjusting the Interpupillary Distance………………………………………
14
3.6 Focusing on the Specimens ………………………………………………
15
3.6.1 Focus Knobs…………………………………………………………
15
3.6.2 Focus Knob Tight Adjustment ………………………………………
15
3.7 Adjusting the Diascopic Illumination Aperture Diaphragm………………
16
3.8 Switching the Objective……………………………………………………
16
3.9 Operation the Phase Contrast Microscope…………………………………
17
3.10 Operation the Emboss Contrast Microscope ……………………………
18
3.10.1 Condenser-side Emboss Contrast Slider……………………………
18
3.10.2 Eyepiece-tube-side Emboss Contrast Slider ………………………
18
3.11 Operating the Episcopic Illumination
19

II
Microscopy………………………………
3.11.1 Eprscopic Illumination………………………………………………
19
3.11.2 Fluorescence Filter Cube Turret ……………………………………
19
3.11.3 Filter Cube Switching Unit …………………………………………
19
3.11.4 Fluorescence Filter Cube……………………………………………
20
3.11.5 Fluorescence LED Unit ……………………………………………
20
3.12 Operating the LCD ………………………………………………………
21
3.12.1 The status of LCD …………………………………………………
21
3.12.2 Operating the illumination switch ………………………………
21
Chapter 4 Assembly………………………………………………………………
22
4.1 Installation diagram ……………………………………………………
22
4.2 Installation Steps ………………………………………………………
23
4.2.1 Installing the Condenser ……………………………………………
23
4.2.2 Installing the Phase Contrast Slider …………………………………
23
4.2.3 Installing the Moving Ruler …………………………………………
23
4.2.4 Installing the Objective ………………………………………………
24
4.2.5 Installing the Eyepiece ………………………………………………
24
4.2.6 Installing the LED Light Box ………………………………………
24
4.2.7 Installing the Camera…………………………………………………
25
4.3 Assembly Episcopic Illumination Microscopy…………………………………
26
4.3.1 Installing the Filter Cube ……………………………………………
26
4.3.2 Installing the Fluorescence LED Unit ………………………………
26
4.3.3 Replacing the Fuse …………………………………………………
26
Chapter 5 Maintenance and Storage……………………………………………
27
5.1 Cleaning …………………………………………………………………
27
5.1.1 Cleaning the Lenses …………………………………………………
27
5.1.2 Cleaning Parts other than Lenses ……………………………………
27
5.1.3 Cleaning off Immersion Oil …………………………………………
28
5.1.4 Decontaminating this Product ………………………………………
28
5.2 Storage ……………………………………………………………………
28
Chapter 6 Troubleshooting ……………………………………………………
29
6.1 Optical System ……………………………………………………………
29
6.2 Mechanical Parts …………………………………………………………
29
6.3 electrical Parts………………………………………………………………
30

Before Using
Safety Symbol
Symbol
Description
The surface is hot and cannot be touched with bare
hands.
Please read this manual carefully before using the
product. Incorrect usage can cause personal injury or
property damage.
The main power switch is turned on.
The main power switch is turned off.
Adjustment direction of illumination.
Ground connection.
ECO
People go lights out switch.
Up and down light switch.
Light switch.
Through the CE certification.
Lever in，Bino：Trino=100：0
Lever out，Bino：Trino=0：100
Safety Precautions
1. Be careful when opening box, prevent lens glass to stick to fingerprint,
perspiration other factors influence observation, prevent lens and other
attachment drop damaged.
2. Avoid placing the microscope in place with direct sunlight, high
temperature or high humidity, dust, and strong vibration. Ensure that
the stage is flat, horizontal and strong enough.
3. If a bacterial solution or water spatters spill on the stage, objective or
observing tube, immediately unplug the power cord. Then,wipe off any
solution or water with to keep the microscope dry. Otherwise, it could
damage the instrument.
4. To avoid blocking the natural convection air for cooling, make sure the
left, right, top, and rear of the microscope are at least 10 centimeters
away from the wall and other objects.

2
5. Grounding the machine to avoid lightning strike.
6. To ensure safety, always make sure the main switch is in "O"
(disconnecting) and cut off the power while waiting for the LED lights
and the lamp room to cool completely. Input voltage check: the input
voltage indicated on the back of the microscope is consistent with the
supply voltage, otherwise the microscope will be seriously damaged.
7. Use the our company dedicated wire.
8. When carrying this product, one hand should firmly hold it by gripping
the recess in the front lower part of the main body and the other holds
the recess in the rear of the main body.
★Do not hold any other parts (such as the upper part of the illumination pillar, focus
knobs, eyepiece tube, or stage) when carrying the microscope. Doing so might result in
dropping or failure of this microscope.
Care and Maintenance
1. All lenses have been calibrated, please do not disassemble by yourself.
2. The structure of objective converter and coarse and fine focus
mechanism are precise, please do not disassemble easily.
3. The instrument should be kept clean, have to regular removal of dust,
special attention should be paid to do not contaminate the optical parts
when cleaning.
4. The stain of the lens, such as fingerprints and grease can be wiped with
a small amount of ethyl ether (70%) and alcohol (30%) mixed solution.
Recess in the rear
Recess in the front lower part
F
F

3
5. Do not use organic solvents to clean the non-optical parts of the
microscope. Please use neutral detergents when cleaning.
6. When using, if the microscope be wet liquid, should immediately cut
off the power and dry.
7. Do not disassemble any part of the microscope, which will affect the
function or reduce the performance of the microscope.
8. The instrument should be placed indoor with cool and dry, covered the
dust cover when not in use. Cover up, must wait for the lamp room to
cool down.
9. The microscope usage environmental requirements:
a) For indoor use;
b) The environment temperature range: 10 ℃~ 35 ℃;
c) Maximum relative humidity: 80% at 31 ℃, linear temperature down
to 34 ℃is 70%, 60% at 37 ℃, 50% at 40 ℃.
10. Microscope to store and transport environment requirements:
a) the environment temperature range: - 40 ℃~ + 70 ℃
b) relative humidity range: 10% ~ 100%
c) atmospheric pressure range: 500 hpa ~ 1060 hpa
Because the solvent such as ether and alcohol are extremely flammable, do
not operate power switch of various electrical equipment when using, and keep away
from open fire, please ensure indoor ventilation.

4
Chapter 1 Introduction
1.1 Technical Specifications
1.1.1 Main technical specifications and configuration
Inverted Biological Microscope,
Inverted LED Fluorescent Microscope
A14.1063
A14.1064
A16.1063
A16.1064
Optical System
NIS60 Infinite Optical System
(F200)
●
●
●
●
Observation
Method
Bright Field,
●
●
●
●
Phase Contrast
●
●
○
○
Epi-Fluorscence
○
○
●
●
DIC 3D Emboss Contrast
○
○
○
○
Hoffman Phase Contrast
○
○
○
○
Head
Seidentopf Viewing Head, Inclined
At 45°, Interpupillary Distance
48-75mm, Eyepiece Tube
Dia.30mm
●
●
●
●
Eyepiece / F.O.V
EW10x/22mm, Diopter Adjustable,
Dia.30mm
●
●
●
●
EW15x/16mm, Diopter Adjustable,
Dia.30mm
○
○
○
○
EW20x/12mm, Diopter Adjustable,
Dia.30mm
○
○
○
○
Nosepiece
Quintuple Nosepiece, Dovetail
Interface
●
-
●
-
Coded Quintuple Nosepiece,
Dovetail Interface
-
●
-
●
LWD Infinity Plan
Objective
4x/0.10, W.D.30.0mm
●
●
○
○
10x/0.25, W.D.10.2mm
○
○
○
○
20x/0.40, W.D.12.0mm
○
○
○
○
40x/0.60, W.D.2.20mm
○
○
○
○
LWD Infinity Plan
Phase Contrast
Objective
4x/0.10, W.D.30.0mm
○
○
○
○
10x/0.25, W.D.10.2mm
●
●
○
○

5
20x/0.40, W.D.12.0mm
●
●
○
○
40x/0.60, W.D.2.20mm
●
●
○
○
LWD Infinity Plan
Semi-APO
Phase Contrast
Objective
4x/0.13, W.D.17.0mm
○
○
○
○
10x/0.3, W.D.7.4mm
○
○
○
○
20x/0.45, W.D.8.0mm
○
○
○
○
40x/0.60, W.D.3.6mm
○
○
○
○
20x/0.45, W.D.7.5-8.8mm With
Iris Adjustable
○
○
○
○
40x/0.60, W.D.3.0-4.4mm With
Iris Adjustable
○
○
○
○
60x/0.70, W.D.1.8-2.6mm With Iris
Adjustable
○
○
○
○
LWD Infinity
Plan
Semi-APO
Fluorescent
Objective
4x/0.13, W.D.17.0mm, Cover
Glass -
-
-
●
●
10x/0.30, W.D.7.4mm, Cover
Glass1.2mm
-
-
●
●
20x/0.45, W.D.8.0mm, Cover
Glass1.2mm
-
-
●
●
40x/0.60, W.D.3.3mm, Cover
Glass1.2mm
-
-
●
●
60x/0.70, W.D.1.8-2.6mm, Cover
Glass1.2mm
○
○
○
○
Focusing System
Coaxial Coarse & Fine
Adjustment, Tension Adjustable
(At Right Hand), Fine Division
0.002mm, Coarse Focusing
Range Up 7mm, Down 1.5mm,
Max Up To 18.5mm After
Removing Focusing Limit.
●
●
●
●
Working Stage
Plain Working Stage 170(X) x
250(Y)mm,
●
●
●
●
Attachable Mechanical Moving
Stage, X-Y Coaxial Moving
128x80mm
●
●
●
●
Stage Clip
●
●
●
●
Well Clamper For Well Plate
●
●
●
●

6
Glass Stage Plate
●
●
●
●
Metal Stage Plate For Culture
Bottle
○
○
○
○
Auxilliary Plate 2 Pieces (1 Piece
Each Side)
●
●
●
●
Universal Holder
●
●
●
●
Terasaki Holder
○
○
○
○
Dia.35mm Petri Dish Holder
○
○
○
○
Dia.54mm Slide & Petri Dish
Holder
○
○
○
○
Dia.65mm Slide & Petri Dish
Holder
○
○
○
○
Dia.90mm Petri Dish Holder
○
○
○
○
Condenser
Long Working Distance
Detachable Condenser NA
0.3, W.D.75mm, Without
Condenser W.D.187mm
●
●
●
●
Illumination
Transmitted Illumination 3W
S-LED Critical Illumination
●
-
●
-
Transmitted Illumination 3W
S-LED Kohler Illumination
-
●
-
●
Reflected Illumination 3W LED,
For Epi-Fluorescence
○
○
●
●
Phase Contrast
Centering Telescope 10x, Tube
Dia. 30mm
●
●
○
○
Phase Slider For 4x//10x-20x-40x
●
●
○
○
Phase Slider For 10x-20x,40x
APO Objectives
○
○
○
○
Phase Slider For 4x,60x APO
Objectives
○
○
○
○
Emboss Contrast
DIC
Emboss Contrast Slider For
10x-20x-40x
Universal Emboss Contrast Plate
For 10x-20x-40x
○
○
○
○
Hoffman Phase
Contrast
Hoffman Phase Contrast Set:
--Hoffman Phase Contrast
Condenser, With Polarizer,
--Hoffman Phase Contrast
Objective 10x, 20x, 40x
○
○
○
○

7
--Hoffman Phase Slider For 10x,
20x, 40x
--Centering Telescope 10x, Tube
Dia. 30mm
Epi-Fluorescence
Attachment
Epi-Fluorescence Attachment,
Turret With 3 Holes For Filter
Cubes, With Noise Terminator
Mechanism, With Attachable UV
Shield
○
○
●
●
Filter Cube B + LED Unit, 365nm
○
○
●
●
Filter Cube G + LED Unit, 405nm
○
○
●
●
Filter Cube U + LED Unit, 485nm
○
○
●
●
Filter Cube V + LED Unit, 525nm
○
○
○
○
Filter Cube FITC
Filter Cube DAPI
Filter Cube TRITC
Photo Port
Head Side Camera Port
Switchable 100/0:0/100
●
●
●
●
Photo Adapter
1.0x C-Mount
●
●
●
●
0.5x C-Mount
○
○
○
○
0.7x C-Mount
○
○
○
○
Power Supply
AC 100-240V,50/60Hz
●
●
●
●
ECO Function
Auto Power Off 15 Minutes After
No Operator Working
○
-
○
-
LCD Screen
LCD Screen On Front Of Body,
Display Using State Of
Microscope, Including
Magnification, Light Intensity,
Standby Status, ECO Set Power
Off Timer 5 Mins to 8 Hours, And
So On.
-
●
-
●
Dimensions
244(W)x543(D)x526(H) mm
●
●
●
●

8
Note：● Standard component, ○ Choose component,― Inapplicability。
Objective Choose component
No.
Technical Specifications
1
Infinity plane semi-apochromatic objective（adjustable）20×、40×、60×
2
Infinity plane semi-apochromatic contrast objective（adjustable）20×、40×、60×
3
Infinity plane semi-apochromatic contrast objective 4×、10×、20×、40×、60×
1.1.2 Objective parameters
Type
A14.1063,A14.1064
A16.1063,A16.1064
Magnification
4×
10×
20×
40×
10×
20×
40×
numerical aperture(N.A)
0.10
0.25
0.40
0.60
0.30
0.45
0.60
Work distance (mm）
30
10.2
12
2.2
7.4
8
3.6
Cover-glass thickness
0.17
0.17
0.17
0.17
1.2
1.2
1.2
Conjugate distance(mm)
∞
∞
∞
∞
∞
∞
∞
1.1.3 Electricity parameter
Item
A14.1063,A14.1064
A16.1063,A16.1064
1
Input voltage
AC100-240V; 50/60 Hz
2
Fuse Size
T500 mA; 250 V
3
LED light
3W S-LED
4
Fluorescence LED B light
—
wavelength 485nm at 3W
5
Fluorescence LED G light
—
wavelength 520nm at 3W
6
Fluorescence LED U light
—
wavelength 360nm at 3W

9
1.2 Components
The microscope A14.1063 components ( following diagrams)，A14.1064
add LCD based on A14.1063.
A14.1063
Eyepiece
Viewing head
Mainframe
Moving stage
Objective
Condenser
Universal holder
Nosepiece
Stage

10
The microscope A16.1064 components ( following diagrams),A16.1063
remove LCD based on A16.1064.
A16.1064
Eyepiece
Viewing head
Mainframe
Universal holder
Condenser
Fluorescence
illumination
Objective
Moving stage
Filter Cube turret
Stage
Nosepiece
LCD

11
Chapter 2 Overview of Agencies
1
Condenser aperture adjustment knob
7
Fine focus knob
2
Contrast slider
8
Coarse focus knob
3
Upper lighting shading part（only FL）
9
Condenser brightness adjuster
4
Stage
10
Up/ down illumination switch（Transmitted
illumination for biological model）
5
Power switch
11
Power indicator
6
Nosepiece
12
Camera mount
2
10
3
4
4
5
11
1
6
7
8
9
12
A16.1063

12
1
Emboss contrast slider
9
Moving stage
2
Fluorescent baffle（only FL）
10
Universal bracket/ holder
3
Elastic adjustment knob
11
Condenser set screw
4
Bracket horizontal movement
knob
5
Bracket vertical movement knob
6
Fuse
7
Outlet
8
Built-in fluorescent light source
part and fuse（only FL）

5
11
1
3
4
7
2
6
10
9
8
A16.1063

13
1
Condenser aperture adjustment knob
9
Fine focus knob
2
Contrast slider
10
Coarse focus knob
3
Universal bracket/ holder set screw
11
intelligent adjuster （only A14.1064
series）
4
Stage bracket/ holder
12
Power indicator
5
Fluorescence cube turret（only FL）
13
Camera mount
6
Power switch
14
LCD（only A14.1064 series）
7
Turntable cover（only FL）
8
Nosepiece clamp screw
2
3
4
4
6
1
8
9
10
7
5
11
12
13
14
A16.1064

14
1
Fluorescent baffle（only FL）
8
Built-in fluorescent light source part
and fuse（only FL）
2
Universal bracket/ holder
9
Nosepiece
3
Emboss contrast slider
10
Moving ruler/ Measuring head
4
Elastic adjustment knob
11
Upper lighting shading part（only FL）
5
Bracket horizontal movement knob
12
Condenser set screw
6
Bracket vertical movement knob
7
Outlet
6
12
4
5
7
8
1
3
9
11
2
10
A16.1064

15
Chapter 3 Regulation and Operation
3.1 Turning the Power on
Turn on the power and turn the main
switch on the back of the microscope
body to "-" (on).
3.2 Turning the Illumination on
3.2.1 Manual lighting switch （for
A14.1063 series）
Make sure the power indicator is on and
press the light switch.
3.2.2 ECO switch (Optional functions)
As shown in figure, the induction device
can sense objects within 0.5m in front of
it.
The blue light is shown, and the light
source of the microscope will not go out,
when one is observing within 0.5m in
front of it. When the person leaves and
without any other object within 0.5m in
front, the blue light is off automatically.
And after 15 minutes, the LED will be off.
Press the ECO function key to open the
lighting again.
Closing ECO: Pressing the ECO function
key until the blue light go out. Recovering
ECO: restart the power.
Illumination
switch
Power switch
Induction device
ECO function key

16
3.3 Adjusting the Illumination
Brightness
As shown in the direction of rotation, the
light source is enhanced, whereas the
light source weakened.
3.4 Specimen Placement and Stage
Knob Adjustment
Load the slide holder into a mechanical
stage, and the cover glass slides
downward slowly into the grooves of the
slide plate.
Rotate the stage knob to place the sample
to the desired position.
CAUTION
Be careful when changing the
objective. The objective may collide
with the specimen, because of the
short working distance of specimen
observation.
3.4.1 Moving stage
The Moving stage is mounted and used
on the plain stage. The specimen can be
moved in the X-axis and Y-axis directions
by operating the stage knobs.
A 128×80 mm micro plate can be placed
on the Moving stage. Attaching the
different specimen holders enables you to
observe various types of specimens.
The upper is the vertical knob, and rotates
in the direction as shown in the figure, the
stage will move forward in the vertical
direction. The lower is the horizontal knob
rotating in the direction shown in the
figure. The stage will move to the left in
the horizontal direction.
X-axis
Y-axis

17
3.4.2 Holders
There are a variety of holders to choose from.
NO.
Model
1
Terasaki micro plate
2
Universal holder
3
54mm Petri dish holder/ Slide holder
4
60 mm Petri dish holder/ Slide holder
5
90 mm Petri dish holder
3.4.3 Auxiliary stage
Connect the auxiliary stage to the left and
right of the stage to expand the stage
width. After connecting the auxiliary
stage, the stage width is 300 mm.
3.5 Adjusting the Interpupillary
Distance
By rotating the binocular part while
looking through the eyepieces, adjust the
distance between the eyepieces so that
the right and left view fields overlap to a
single image from eyepieces
Auxiliary stage