Pyxis ST-500 User manual

ST-500 Series Inline PTSA Fluorometer
Probes
User Manual
March 23, 2020
Rev. 3.02
Pyxis Lab, Inc.
1729 Majesc Dr. Suite 5
Lafayee, CO 80026 USA
www.pyxis-lab.com
© 2017 Pyxis Lab, Inc.
Pyxis Lab Proprietary and Condenal

Table of Contents
1 Introducon 2
2 Specicaons 2
3 Unpacking Instrument 3
3.1 StandardAccessories ..................................... 3
3.2 OponalAccessories ..................................... 3
4 Installaon 4
4.1 ST-500andST-500ROPiping ................................. 4
4.2 ST-500SSPiping ........................................ 5
4.3 Wiring............................................. 6
4.4 ConnecngviaBluetooth................................... 6
4.5 ConnecngviaUSB ...................................... 6
5 Setup and Calibraon with uPyxis® Mobile App 7
5.1 Download uPyxis® MobileApp................................ 7
5.2 Connecng to uPyxis® MobileApp.............................. 7
5.3 Calibraon Screen and Reading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
5.4 DiagnosisScreen ....................................... 9
5.5 DeviceInfoScreen....................................... 10
6 Setup and Calibraon with uPyxis® Desktop App 10
6.1 Install uPyxis® DesktopApp.................................. 10
6.2 Connecng to uPyxis® DesktopApp ............................. 11
6.3 InformaonScreen ...................................... 11
6.4 CalibraonScreen....................................... 12
6.5 DiagnosisScreen ....................................... 14
7 Communicaon using Modbus RTU 14
8 Sensor Maintenance and Precauon 14
8.1 Methods to Cleaning ST Series Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
8.2 Storage ............................................ 15
9 Troubleshoong 16
10 Contact Us 16

Warranty Informaon
Condenality
The informaon contained in this manual may be condenal and proprietary and is the property of Pyxis
Lab, Inc. Informaon disclosed herein shall not be used to manufacture, construct, or otherwise reproduce
the goods described. Informaon disclosed herein shall not be disclosed to others or made public in any
manner without the express wrien consent of Pyxis Lab, Inc.
Standard Limited Warranty
Pyxis Lab warrants its products for defects in materials and workmanship. Pyxis Lab will, at its opon, repair
or replace instrument components that prove to be defecve with new or remanufactured components
(i.e., equivalent to new). The warranty set forth is exclusive and no other warranty, whether wrien or oral,
is expressed or implied.
Warranty Term
The Pyxis warranty term is thirteen (13) months ex-works. In no event shall the standard limited warranty
coverage extend beyond thirteen (13) months from original shipment date.
Warranty Service
Damaged or dysfunconal instruments may be returned to Pyxis for repair or replacement. In some in-
stances, replacement instruments may be available for short duraon loan or lease.
Pyxis warrants that any labor services provided shall conform to the reasonable standards of technical com-
petency and performance eecve at the me of delivery. All service intervenons are to be reviewed and
authorized as correct and complete at the compleon of the service by a customer representave, or des-
ignate. Pyxis warrants these services for 30 days aer the authorizaon and will correct any qualifying
deciency in labor provided that the labor service deciency is exactly related to the originang event. No
other remedy, other than the provision of labor services, may be applicable.
Repair components (parts and materials), but not consumables, provided during a repair, or purchased
individually, are warranted for 90 days ex-works for materials and workmanship. In no event will the in-
corporaon of a warranted repair component into an instrument extend the whole instrument’s warranty
beyond its original term.
Warranty Shipping
A Repair Authorizaon (RA) Number must be obtained from Pyxis Technical Support before any product can
be returned to the factory. Pyxis will pay freight charges to ship replacement or repaired products to the
customer. The customer shall pay freight charges for returning products to Pyxis. Any product returned to
the factory without an RA number will be returned to the customer. To receive an RMA you can generate a
request on our website at hps://pyxis-lab.com/request-tech-support/.
Pyxis Technical Support
Contact Pyxis Technical Support at [phone], [email protected], or by lling out a request for
support at hps://pyxis-lab.com/request-tech-support/.
ST-500 Series User Manual [email protected] | [phone]

1 Introducon
The Pyxis ST-500 series inline uorometer probes measure the concentraon of uorescence tracer PTSA
(pyrenetetrasulfonic acid) in water. The standard ST-001 Tee Assembly provided with each ST-500/ST-500RO
sensor, has two 3/4” female NPT ports and can be placed to an exisng 3/4” sample water line. Pyxis Lab
also oers 2” and 3” Tee formats for larger ow installaons. The 4-20mA current output of the ST-500
probe can be connected to any controller that accepts an isolated or non-isolated 4-20mA input. The ST-
500 series probes are smart devices. In addion to measuring uorescence, the ST-500 series probes have
extra photo-electric components that monitor the color and turbidity of the sample water. This extra fea-
ture allows automac color and turbidity compensaon to eliminate interference commonly associated
with real-world waters.
The Pyxis ST-500 series probes have a short uidic channel and can be easily cleaned. The uidic and op-
cal arrangement of the ST-500 series probes are designed to overcome shortcomings associated with other
uorometers that have a distal sensor surface or a long, narrow uidic cell. Tradional inline uorometers
are suscepble to color, turbidity interference, and fouling, making them very dicult to properly clean.
The Pyxis ST-500 series probes use a narrow wavelength band gallium phosphide photodiode and high
temperature-tolerant and humidity-resistant opcal lters. This combinaon greatly enhances the robust-
ness of the probes. It can be operated under a wide range of ambient condions without the need of
humidity and temperature regulaon. The performance of the ST-500 series probes can be stable and con-
sistent for a long period of me.
2 Specicaons
Specicaon*ST-500 ST-500RO ST-500SS
P/N 50661 50669 50700
Range (ppb) 0-300 0-40 0-300
Resoluon (ppb) 0.01
Accuracy ±1% of reading
Calibraon Two-point calibraon against standard soluon
Outputs 4-20mA Analog Output, RS-485 Digital Output with Modbus protocol
Installaon†Custom tee assembly (P/N: ST-001) with 3/4” female socket & NPT threaded ports
Cable Length 5  with IP67 connectors
Power Supply 22-26 VDC, 1 W
Dimension inch (mm)‡Length: 6.8 (172.7), Body Diameter: 1.44 (36.6)
Weight lbs (g) 0.37 (170) 0.37 (170) 2.5 (1130)
Material CPVC CPVC 304 Stainless Steel
Operaonal Tempera-
ture °F (°C) 40-104 (4-40) 40-104 (4-40) 32-104 (0-40)
Storage Temperature °F
(°C) 20-140 (-7-60)
Pressure psi (MPa) Up to 100 (0.7) Up to 100 (0.7) Up to 290 (2.0) at 149 °F (65 °C)
Enclosure Rang IP67
Regulaon CE
*With Pyxis’s connuous improvement policy, these specicaons are subject to change without noce.
†The ST-500SS probe does not come with the custom tee assembly (P/N: ST-001). It has 3/4” female NPT threaded ports on the probe
itself.
‡See Figure 4 for ST-500SS dimensions.
ST-500 Series User Manual [email protected] | [phone]

3 Unpacking Instrument
Remove the instrument and accessories from the shipping container and inspect each item for any damage
that may have occurred during shipping. Verify that all accessory items are included. If any item is missing
or damaged, please contact Pyxis Lab Customer Service at [email protected].
3.1 Standard Accessories
• Tee Assembly 3/4” NPT (1x Tee, O-ring, and Nut) P/N: ST-001
• 7-Pin Female Adapter/Flying Leads Cable (2 ) P/N: MA-1100
• User Manual available online at www.pyxis-lab.com/support.html
3.2 Oponal Accessories
Figure 1.
ST-500 Series User Manual [email protected] | [phone]

Figure 2.
4 Installaon
4.1 ST-500 and ST-500RO Piping
The provided ST-001 Tee Assembly can be connected to a pipe system through the 3/4” female ports, either
socket or NPT threaded. To properly install the ST-500 series probe into the ST-001 Tee Assembly, follow
the steps below:
1. Insert the provided O-ring into the O-ring groove on the tee.
2. Insert the ST-500 series probe into the tee.
3. Tighten the tee nut onto the tee to form a water-ght, compression seal.
Figure 3. Dimension of the ST-500/ST-500RO and the ST-001 Tee Assembly (mm)
ST-500 Series User Manual [email protected] | [phone]

4.2 ST-500SS Piping
The ST-500SS probe has 3/4” female NPT threaded ports on the probe itself and therefore does not require
a custom tee assembly. It is recommended that two 3/4” NPT to 1/4” tubing adapters are used to connect
the probe to the sampling system. Sample water entering the probe must be cooled down to below 104 °F
(40 °C). The probe can be held by a 1.75-inch pipe clamp or mounted to a panel with four 1/4-28 bolts. See
Figure 4 for ST-500SS dimensions.
Figure 4. Dimension of the ST-500SS (inch)
ST-500 Series User Manual [email protected] | [phone]

4.3 Wiring
If the power ground terminal and the negave 4-20mA terminal in the controller are internally connected
(non-isolated 4-20mA input), it is unnecessary to connect the 4-20mA negave wire (green) to the 4-20mA
negave terminal in the controller. If a separate DC power supply other than that from the controller is
used, make sure that the output from the power supply is rated for 22-26 VDC @ 65mA.
*NOTE*The negave 24V power terminal (power ground) and the negave 4-20mA ter-
minal on the ST-500 series probe are internally connected.
Follow the wiring table below to connect the ST-500 series probe to a controller:
Wire Color Designaon
Red 24V +
Black 24V Power ground
White 4-20mA +
Green*4-20mA -
Blue RS-485 A
Yellow RS-485 B
Clear Shield, earth ground
*Internally connected to the power
ground
4.4 Connecng via Bluetooth
A Bluetooth adapter (P/N: MA-WB) can be used to connect an ST-500 series probe to a smart phone with
the uPyxis® Mobile App or a computer with the uPyxis® Desktop App.
Figure 5. Bluetooth connecon to ST-500 series probe
4.5 Connecng via USB
A USB-RS485 adapter (P/N: MA-485) can be used to connect an ST-500 series probe to a computer with the
uPyxis® Desktop App.
*NOTE*Using non-Pyxis USB-RS485 adapters may result in permanent damage of the ST-
500 series probe communicaon hardware.
ST-500 Series User Manual [email protected] | [phone]

Figure 6. USB connecon to ST-500 series probe
5 Setup and Calibraon with uPyxis® Mobile App
5.1 Download uPyxis® Mobile App
Download uPyxis® Mobile App from Apple App Store or Google Play.
Figure 7.
5.2 Connecng to uPyxis® Mobile App
Turn on Bluetooth on your mobile phone (Do not pair the phone Bluetooth to the ST-500 series probe).
Open uPyxis® Mobile App. Once the app is open the app will start to search for the sensor. Once the
uPyxis® Mobile App connects to the sensor, press the ST-500 series probe.
ST-500 Series User Manual [email protected] | [phone]

Figure 8.
5.3 Calibraon Screen and Reading
When connected, the uPyxis® Mobile App will default to the Calibraon screen. From the Calibraon
screen, you can perform calibraons by pressing on Zero Calibraon,Slope Calibraon, and 4-20mA Span.
Follow the screen instrucons for each calibraon step.
Figure 9.
ST-500 Series User Manual [email protected] | [phone]

5.4 Diagnosis Screen
From the Diagnosis screen, you can check the diagnosis condion as well as Export & Upload. This feature
may be used for technical support when communicang with ser[email protected].
To preform a probe cleaniness check, rst select the Diagnosis Condion which denes the uid type that
the ST-500 series probe in currently measuring, then press Cleanliness Check. If the probe is clean, a green
Clean message will be shown. If the probe is parally fouled, a yellow Becoming Dirty message will
be shown. If the probe is severely fouled, a red Dirty message will be shown. In this case, follow the
procedure in the Methods to Cleaning ST Series Probe secon of this manual.
Figure 10.
ST-500 Series User Manual [email protected] | [phone]

5.5 Device Info Screen
From the Device Info screen. You can name the Device or Product.
Figure 11.
6 Setup and Calibraon with uPyxis® Desktop App
6.1 Install uPyxis® Desktop App
Download the latest version of uPyxis® Desktop soware package from: hp://www.pyxis-lab.com/sup-
port.html this setup package will download and install the Microso.Net Framework 4.5 (if not previously
installed on the PC), the USB driver for the USB-Bluetooth adapter (MA-NEB), the USB-RS485 adapter (MA-
485), and the main uPyxis® Desktop applicaon. Double click the uPyxis.Setup.exe le to install.
Figure 12.
Click Install to start the installaon process. Follow the screen instrucons to complete the USB driver and
uPyxis® installaon.
ST-500 Series User Manual [email protected] | [phone]

6.2 Connecng to uPyxis® Desktop App
When the uPyxis® Desktop App opens, to nd your device, click on Device, then Connect via USB-RS485.
Figure 13.
6.3 Informaon Screen
Once connected to the device, a picture of the device will appear on the top le corner of the window and
the uPyxis® Desktop App will default to the Informaon screen. On the Informaon screen you can set the
informaon descripon for Device Name and Product Name, then click Set to save.
Figure 14.
ST-500 Series User Manual [email protected] | [phone]

6.4 Calibraon Screen
To calibrate the device, click on Calibraon. On the Calibraon screen there are three calibraon tabs, Zero
Calibraon,Slope Calibraon, and 4-20mA Span. The screen also displays the reading of the device. The
reading refresh rate is every 4 seconds.
Figure 15.
6.4.1 Zero Calibraon
To perform Zero Calibraon, click on Zero Calibraon. Then follow the instrucon on how to calibrate, then
click Ok.
Figure 16.
ST-500 Series User Manual [email protected] | [phone]

6.4.2 Slope Calibraon
To perform Slope Calibraon, click on Slope Calibraon. Then follow the instrucon on how to calibrate,
then click Slope Calibraon.
Figure 17.
6.4.3 4-20mA Span
To perform 4-20mA Span, click on 4-20mA Span. Then follow the instrucons provided to alter the 4-20mA
output span of the sensor, then click Set 20mA Span. Each sensor format will have a maximum 20 mA range
allowed. 4-20mA Span setup must be maintained within the limit of its respecve sensor.
Figure 18.
ST-500 Series User Manual [email protected] | [phone]

6.5 Diagnosis Screen
Aer the device has been calibrated and installaon has been completed, to check diagnosis, click on Diag-
nosis. When in the Diagnosis screen you can view the Diagnosis Condion of the device. This feature may
be used for technical support when communicang with [email protected].
To preform a probe cleaniness check, rst select the Diagnosis Condion which denes the uid type that
the ST-500 series probe in currently measuring, then click Cleanliness Check. If the probe is clean, a green
Clean message will be shown. If the probe is parally fouled, a yellow Becoming Dirty message will
be shown. If the probe is severely fouled, a red Dirty message will be shown. In this case, follow the
procedure in the Methods to Cleaning ST Series Probe secon of this manual.
Figure 19.
7 Communicaon using Modbus RTU
The ST-500 series probe is congured as a Modbus slave device. In addion to the ppb PTSA value, many
operaonal parameters, including warning and error messages, are available via a Modbus RTU connecon.
Contact Pyxis Lab Customer Service (ser[email protected]) for more informaon.
8 Sensor Maintenance and Precauon
The ST-500 series probe is designed to provide reliable and connuous PTSA readings even when installed
in moderately contaminated industrial cooling waters. Although the opcs are compensated for the eects
of moderate fouling, heavy fouling will prevent the light from reaching the sensor, resulng in low readings
and the potenal for product overfeed if the ST-500 series probe is used as part of an automated control
system. When used to control product dosing, it is suggested that the automaon system be congured
to provide backup to limit potenal product overfeed, for example by liming pump size or duraon, or by
alarming if the pumping rate exceeds a desired maximum limit.
The ST-500 series probe is designed to be easily removed, inspected, and cleaned if required. It is suggested
that the ST-500 series probe be checked for fouling and cleaned/calibrated on a monthly basis. Heavily con-
taminated waters may require more frequent cleanings. Cleaner water sources with less contaminaon
ST-500 Series User Manual [email protected] | [phone]

may not require cleaning for several months.
The need to clean the ST-500 series probe can be determined by the Cleanliness Check using either the
uPyxis® Mobile App (see the Mobile Diagnosis Screen secon) or the uPyxis® Desktop App (see the Desk-
top Diagnosis Screen secon).
8.1 Methods to Cleaning ST Series Probe
Any equipment in contact with industrial cooling systems is subject to many potenal foulants and con-
taminants. Our inline probe cleaning soluons below have been shown to remove most common foulants
and contaminants. A small, so bristle brush, Q-Tips coon swab, or so cloth may be used to safely clean
the probe housing and the quartz opcal sensor channel. These components and more come with a Pyxis
Lab Inline Probe Cleaning Soluon Kit (P/N: SER-01) which can be purchased at our online Estore/Catalog
hps://pyxis-lab.com/product/st-series-probe-cleaning-kit/
Figure 20. Inline Probe Cleaning Soluon Kit
To clean the ST series probe, soak the lower half of the probe in 100 mL inline probe cleaning soluon for
30 minutes. Rinse the ST series probe with dislled water and then check for the ashing blue light inside
the ST series probe quartz tube. If the surface is not enrely clean, connue to soak the ST series probe for
an addional 30 minutes. Use the small, so bristle brush and Q-Tips coon swabs as necessary to remove
any remaining contaminants in the ST series probe quartz tube.
8.2 Storage
Avoid long term storage at temperature over 100 °F. In an outdoor installaon, properly shield the ST-500
series probe from direct sunlight and precipitaon.
ST-500 Series User Manual [email protected] | [phone]

9 Troubleshoong
If the ST-500 series probe output signal is not stable and uctuates signicantly, make an addional ground
connecon – connect the clear (shield, earth ground) wire to a conductor that contacts the sample water
electrically such as a metal pipe adjacent to the ST-500 series tee.
Carry out roune calibraon vericaon against a qualied PTSA standard. Aer properly cleaning the
ST-500 series sensor, carry out the zero point calibraon with dislled water and slope calibraon using
the qualied PTSA standard. Pyxis Lab PTSA Standards can be purchased at our online Estore/Catalog
hps://pyxis-lab.com/product/ptsa-100/
Figure 21. PTSA 100 Standard
10 Contact Us
Pyxis Lab, Inc
1729 Majesc Dr. Suite 5
Lafayee, CO 80026 USA
www.pyxis-lab.com
Phone: [phone]
Email: [email protected]
ST-500 Series User Manual [email protected] | [phone]