Sorvall Legend T User manual
Instruction Manual
Legend™ T / RT
SO
R
V
AL
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®
EASYset™
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How to use this manual
Please, use this manual to get acquainted with your
centrifuge and its accessories.
The manual helps you to avoid inappropriate handling.
Make sure to always keep it close to the centrifuge.
A manual that is not kept handy cannot provide aid
against improper handling and thus against dam-
age to individuals and surrounding.
The manual contains chapters on
• Safety regulations
• Instrument description
• Rotors and accessories
• Transportation and installation
• Use of the centrifuge
• Maintenance and care
• Troubleshooting
• Technical data
• Index
Unfold, please
On the overleaf is a graphic
representation of the control panel and
a description of the control functions.
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acceleration
profiles
deceleration
profiles
"set" keys
Quick run
speed/RCF run time
open lid
stop
start
rpm/RCF
switch key
display
program selection
"set" keys
(only for instruments with refrigeration unit)
temperature
Bucket selecti-
on
The mains switch is located on the right-hand side
panel.
Pressed up = ON I
Pressed down = OFF 0
pre-temperature-regulation
Before switching on the centrifuge
please read this manual
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Control panel of the Legend™ T / RT
Display panels
Program selection
Key 1 - 4 : store or recall programs.
Acceleration profiles (1= slowest ...... 9= fastest)
permanent
display: acceleration profile last set 1- 9
Deceleration profiles (1= slowest ...... 9= fastest)
permanent
display: deceleration profile last set 1- 9
Speed / RCF
run: current value of speed or RCF after activation of
switch key
end: "End"
lid open: "OPEN"
"Lift Lid" (if lid is not automatically lifted off)
(before start)
lid closed: "0" with flashing point
(rotor not yet identified)
error code: will flash in display
Run time
time selection: - remaining run time to 0
continuous - run time passed (HLD)
operation (hld) (in hours, minutes)
"Quick Run": - run time passed (as long as button is held;
in minutes and seconds)
Temperature*
run: current sample temperature in °C
(in temperature equilibrium)
* only for instruments with refrigeration unit
Keys
start : normal start of the centrifuge
stop : manual stop of a run
open lid: open lid (possible only with the instrument
switched on)
Quick run: short-term operation of the centrifuge as long as
key remains pressed
rpm/RCF
switch: switching between rpm and RCF display
Bucket set.: Setting of the bucket number
Pretemp : Pre-temp-function*
"set" keys: stepwise increase/decrease of setpoint values
Short pressing of any of the "set" keys: switch from current to
preset value, signalled by flashing display.
Error codes (troubleshooting see chapter "Troubleshooting"):
E-19: Unpermissible rotor
E-20: Rotor not identified
E-29: Motor or rotor blocked
E-31: Overtemperature in motor
E-32: Overtemperature in electronic
E-33: Excessive pressure in the refrigeration unit
E-34: Overvoltage
E-35: Overcurrent
Lift Lid : Smoothly lift the lid
rotor: set speed higher than permissible speed of
the rotor
bAL : unbalance
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Contents
1
Contents
For your safety........................................... 3
Proper use...............................................................3
Improper use ...........................................................3
Centrifuging hazardous materials ...........................3
Handling the centrifuge ...........................................4
Conformity to current standards..............................5
Safety instructions in this manual ...........................5
The Legend™ T / RT.................................. 7
Description ..............................................................7
Safety systems........................................................8
Parts supplied .........................................................8
Function and features .............................................9
Accessories ............................................. 13
Rotors for the Legend™ T ....................................14
Rotors for the Legend™ RT..................................18
Before use................................................ 27
Centrifuge transport and installation .....................27
Proper location ......................................................27
Main connection ....................................................28
Operation..................................................29
Switching on the centrifuge................................... 29
Actuating the lid .................................................... 29
Opening the lid.................................................. 29
Closing the lid.................................................... 29
Installing the rotor ................................................. 30
Loading the rotor................................................... 36
Maximum loading.............................................. 36
Filling the centrifuge tubes ................................ 37
Maximum permissible load difference .............. 37
Inserting the centrifuge tubes............................ 38
Aerosol-tight operation......................................... 40
Checking for aerosol-tight bio-containment ...... 43
Entering parameters ............................................. 45
Deceleration curves .............................................. 45
Switching from speed to RCF display................... 45
Bucket selection for swinging bucket rotors ......... 45
Selecting speed .................................................... 45
Entering the RCF value ........................................ 46
More about the RCF value................................ 47
Selecting run time ................................................. 47
Run time selection............................................. 47
Continuous operation........................................ 48
Extended time mode ......................................... 48
Selecting the temperature..................................... 49
Pretemp function................................................... 49
Starting the centrifuge........................................... 50
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Contents
2
Imbalance display..................................................50
Changing the settings during the run ....................50
Stopping the centrifuge .........................................51
Stopping with preset run time............................51
Stopping with continuous operation ..................51
Temperature control during standby .....................51
Working with programs .........................................52
Program display.................................................52
Entering/changing a program ............................52
Centrifuging with a program ..............................52
”Quick Run” ...........................................................53
Removing the rotor................................................53
Audible alarm ........................................................54
Turning the centrifuge off ......................................54
Maintenance and care .............................55
Routine maintenance to be performed by the
customer................................................................55
Cleaning.............................................................55
Disinfection ........................................................56
Decontamination................................................58
Autoclaving ........................................................58
The KENDRO service offering ..............................59
Warranty conditions ..............................................59
Troubleshooting.......................................61
Emergency lid release ..........................................61
Error troubleshooting ............................................63
Contacting Kendro Service ...................................72
Technical Data..........................................73
Electrical connections / fuses ...............................75
Appendix...................................................77
Acceleration and deceleration profiles..................77
Speed/RCF diagrams ...........................................87
Index .........................................................93
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For your safety
3
For your safety
The SORVALL®centrifuges are manufactured ac-
cording to current technical standards and regula-
tions. Nonetheless, centrifuges may pose danger to
individuals and surrounding if
• they are not used as designed
• they are operated by untrained personnel
• their design is improperly changed
• the safety instructions are not followed
Therefore, personnel involved with operation and
maintenance of the centrifuge must read and
follow the safety instructions.
In addition, the pertinent regulations for prevention of
accidents must be strictly followed.
This manual is an integral part of the cen-
trifuge assembly and must be kept close
at hand at all times.
Proper use
The centrifuge is designed to separate liquid-
suspended materials having different densities and
particle size, respectively (maximum sample density
is 1.2 g/cm³ {ml} at maximum speed).
Improper use
During a run, a safety zone of 30 cm (12 inches)
around the centrifuge must be maintained where
neither persons nor hazardous materials may be
stationed.
The centrifuge may cause harm to user or others or
may damage instruments and samples if safety
measures are not followed:
Centrifuging hazardous materials
• The centrifuge is neither made inert, nor is it ex-
plosion-proof. Therefore never use the centrifuge
in an explosion-prone environment.
• Explosive or flammable substances must not be
centrifuged. The same holds for substances
prone to react violently with each other.
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For your safety
4
• Do not centrifuge toxic or radioactive substances
or pathogenic microorganisms unless you have
taken proper precautions.
If microbiological samples of risk group II (accord-
ing to "Laboratory Biosafety Manual" of WHO) are
being centrifuged, aerosol-tight bioseals have to
be used.
For materials with a higher risk group, a secon-
dary containment is required.
• Should toxins or pathogenic substances enter the
centrifuge or its parts, you must perform the
proper procedures for disinfection (see "Mainte-
nance and care – Disinfection").
• Strongly corrosive substances that may cause
damage to materials and reduce the mechanical
strength of the rotor, may be centrifuged only in-
side protective tubes.
Handling the centrifuge
• Use only original accessories for the centrifuge.
The only exception are common glass or plastic
centrifuge tubes, if they are approved for the rotor
speed and RCF values then they can be used.
• Never use the centrifuge unless the rotor is prop-
erly installed.
• You may use the centrifuge only with a properly
loaded rotor. You must not overload the rotor.
• Strictly follow the rules and regulations for clean-
ing and disinfection
• If the rotor or the rotor cover shows signs of cor-
rosion or wear, you must stop using it.
• Never manually open the lid if the rotor still turns.
• You may use the emergency lid release only in
case of emergency, e.g. during a power failure
(see chapter "Troubleshooting“).
• Never use the centrifuge with the lid open.
• Never use the centrifuge if the front panel has
been partially or totally removed.
• Changes in mechanical or electrical components
of the centrifuge may be carried out only by indi-
viduals authorized by Kendro Laboratory Prod-
ucts.
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For your safety
5
Conformity to current standards
SORVALL®centrifuges are manufactured and tested
according to the following standards and regulations:
for all voltages:
• IEC 1010-1 / EN 61010-1
• IEC 1010-2 / EN 61010-2-020
− Pollution degree 2
− Overvoltage category II
for 120 V only:
• CAN/CSA-C22.2 No. 1010.1-92
• CAN/CSA-C22.2 No. 1010.2.020-94
Safety instructions in this manual
This symbol denotes potential hazards
to persons.
This symbol denotes potential damage
to the centrifuge or parts in its immedi-
ate surroundings.
General hints are marked with this sym-
bol.
In addition, you are asked to adhere to the pertinent
regulations, in Germany
• Regulations for prevention of accidents VBG 4
• Regulations for prevention of accidents VBG 5
• Regulations for prevention of accidents VBG 7z
• Regulations for prevention of accidents VBG 20
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For your safety
6
Notes
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Description of the Legend™
7
The Legend™ T / RT
The figure below shows a Legend™ RT with the lid
open and a swinging bucket rotor installed.
Description
The Legend™ RT is a general-purpose tabletop
centrifuge for biotechnological and pharmaceutic
research that moves high capacity centrifugation
onto the fast track. It spins more tubes, at higher
RCFs, more rapidly than competitive instruments,
and can process nominally three liters of sample in a
single run. There are various rotors with high RCF
values that can accommodate a wide range of ac-
cessories for all common tube types, microtiter
plates and deep-wells.
The user-friendly "EASYset™" control panel permits
easy selection of speed, RCF value, run time and
run profile (acceleration and deceleration), as well as
temperature of the Legend™ RT. You can switch
from speed to RCF display and vice versa, with a
touch of a button and even during a run.
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Description of the Legend™
8
Safety systems
The Legend™ is equipped with a number of safety
systems:
• Housing and lid is constructed of 8 mm steel.
• Lid with window
• Lid lock with safety check
You can open the centrifuge lid only when the
power is turned on and the rotor has come to a
stop. You can start the centrifuge only if the lid is
properly locked.
• Automatic rotor identification
• Electronic imbalance detection as a function of
rotor (SMARTspinTM)
Do not tamper with the safety
system!
Parts supplied
Accessories supplied with the centrifuge are:
− power cord
− a special wrench for securing the rotor
(as seen in picture)
− condensed operating instructions
− corrosion protective oil
The printed documents consist of the delivery notes
and this Manual
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Description of the Legend™
9
Function and features
Basic unit/ function Description / feature
Cabinet / frame galvanized steel
Chamber stainless steel
Drive Brushless induction drive
Key pad and display key pad and display elements covered by an easy-care continues surface
Control Microprocessor driven by EASYsetTM
Main memory Recalls last run parameters
Program memory data are stored until new values are entered.
Advanced features RCF-programming, quick run, pretemp *, temperature control during standby
Acceleration and deceleration pro-
files
9 acceleration and 9 deceleration profiles
Rotor identification automatic
SMARTspinTM
imbalance detection system
electronic, effective as a function of rotor and speed
Soft touch lid lock motor assisted lid locking
( * only with refrigeration unit )
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Description of the Legend™
10
The EASYset™ user interface
Function Feature
Program memory keys freely programmable
Acceleration / deceleration profile 1 = slowest, ... 9 = fastest acceleration / deceleration curve
Setting speed by rpm adjustable from 300 rpm to 15 000 rpm, in 10 rpm increments
RCF selection upon activation of RCF switch , the RCF value can then be entered
Time selection adjustable in minutes from 1 min to 9 h 59 min,
or extended time mode from 1 min to 99 h,
”hld“-mode: continuous operation
Run time display in "quick run“ mode between 1 s and 60 s in seconds´ steps, above in minutes´ steps
Setting temperature * adjustable from -9°C to +40°C, in one degree increments
End of centrifugation the speed display will read "End“
( * only with refrigeration unit)
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Description of the Legend™
11
Function Feature
Lid opening automatic unlocking via ”Open lid“ key (
)
(unlocking in case of power failure: see chapter ”Troubleshooting“)
Start start key (
)
Stop stop key ( )
"Quick Run“ mode pressing the "Quick Run“ key (
) activates maximum acceleration up to the
maximum permissible speed of rotor; upon key release centrifuge stops with
maximum deceleration power.
Diagnostic messages
• alternating display ”Rotor“/maximum speed or RCF
(speed selected exceeds max. speed of the rotor)
• lid has not been lifted off the lock during opening:
display ”Lift lid“ (manual lifting of lid required)
• general instrument malfunction
(error messages with ERROR codes, see “Troubleshooting“)
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Description of the Legend™
12
Notes
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Rotors and accessories
13
Accessories
A rotor is not included as part of a Legend™ T or RT
centrifuge.
A large variety of rotors are available as accessories.
In addition, there are adapters and reduction sleeves
for a variety of commercially available tubes and
bottles.
For a complete list of available tubes, bottles, adapt-
ers, and accessories refer to our current sales
documentation.
For more information you can visit our web site at
http://www.Kendro.com
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Rotors and accessories
14
Rotors for the Legend™ T
Table 1: Rotors for Legend™ T 100V / 120V (differences of 230V instruments are shown in parentheses)
Rotor designation Swinging Bucket Rotor 7500 6445
(7500 6434 with integrated locking mechanism)
With bucket
Order no.
Round bucket
750 ml
7500 6441
Rectangular bucket
250 ml
7500 6446
Carrier for
microplates
7500 6449
Maximum permissible load [ g ] 4 x 800 4 x 750 4 x 500
Maximum speed nmax [ rpm ] 4150 ( 4350 ) 3750 ( 4350 ) 4150 ( 4350 )
Maximum RCF value at nmax 3696 ( 4083 ) 2986 ( 4020 ) 3061 ( 3364 )
Radius (max.) [ cm ] 19.2 19.0 15.9
Acceleration / deceleration time [ s ] 60 / 30 ( 40 / 40 ) 60 / 30 ( 45 / 40 ) 50 / 25 (40 / 35 )
Heating of samples at nmax
relative to room temperature 23°C,
run time 1 hour
7°C ( 9°C ) 7°C ( 10°C ) 4°C ( 5°C )
Aerosol-tight * yes (with cap 75006421) yes (with cap 75006448) no
Autoclavable 121°C 121°C 121°C
* Checked by TÜV (Technical Supervision Association) Product Service GmbH - Hamburg
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Rotors and accessories
15
Table 1: Rotors for Legend™ T 100V / 120V (differences of 230V instruments are shown in parentheses)
Rotor designation Swinging Bucket Rotor 7500 6445
(7500 6434 with integrated locking mechanism)
With bucket
Order no.
Tissue culture bucket
250 ml
7500 6497
Multiple carriers
25 x 5 / 7 ml
7500 6470
Multiple carriers
20 x 10 / 15 ml
7500 6471
max. tube dimension d1) x l [ mm ] 61 x 180 14 x 120 17 x 140
Maximum permissible load [ g ] 4 x 325 4 x 400 25 x 16 4 x 520 20 x 26
Maximum speed nmax [ rpm ] 3750 ( 4350 ) 3750 ( 4350 ) 3750 ( 4350 )
Maximum RCF value at nmax 3348 ( 4506 ) 2814 ( 3787 ) 3018 ( 4062 )
Radius (max.) [ cm ] 21.3 17.9 19.2
Acceleration / deceleration time [ s ] 50 / 25 ( 35 / 35 ) 50 / 25 ( 40 / 40 ) 55 / 27 ( 45 / 40 )
Heating of samples at nmax
relative to room temperature 23°C,
run time 1 hour
9°C ( 11°C ) 8°C ( 10°C ) 8°C ( 10°C )
Aerosol-tight * no no no
Autoclavable 121°C 121°C 121°C
1) d = diameter
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Rotors and accessories
16
Table 1: Rotors for Legend™ T 100V / 120V (differences of 230V instruments are shown in parentheses)
Rotor designation
Order no.
BIOshield®Rotor
4 x 250 ml
7500 6435
Highplate®Rotor
2 x 5 plates
7500 6444
Maximum permissible load [ g ] 4 x 600 2 x 500
Maximum speed nmax [ rpm ] 5350 5250
Maximum RCF value at nmax 5824 5083
Radius (max.) [ cm ] 18.2 16.5
Acceleration / deceleration time [ s ] 145 / 70 ( 85 / 70 ) 110 / 55 ( 85 / 60 )
Heating of samples at nmax
relative to room temperature 23°C,
Run time 1 hour
10°C 13°C
Aerosol-tight * yes yes
Autoclavable 121°C no
* Checked by TÜV (Technical Supervision Association) Product Service GmbH - Hamburg
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1
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