Thermo Scientific RMI300S-9-A Operating instructions

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User Manual
Midi 40 Series
CO2Incubator
Operating and Maintenance Manual 7003403 Rev. 9

Thermo Scientific
MANUAL NUMBER 7003403
9 41116/IN-4785 11/30/16
Replaced Sample Port and CO2 Inlet labels on pg 3-4
ccs
8 40639 3/22/16 Updated to UL requirements, added risk assessments ccs
7 30073/IN-4491 7/7/15 Added stacking hardware kit to Parts List ccs
6 30073/IN-4491 7/28/14 Clarified specs in Location for stacking ccs
5 26439/IN-4050 2/5/13 Updated uniformity in Specifications ccs
4 28251/IN-4219 8/30/12 Removed RS-485 ccs
3 28728 6/18/12 Updated Calibration sections - pg 6-4 through 6-6 ccs
2 27796/IN-4127 10/3/11 Tubing size from 3/16 to 1/4” - pgs 4-1 and 4-3 ccs
-- -- 6/10/10 Corrected typo in Table 6-1 on pg 6-2 - removed O2 (per D. Wernerspach) ccs
Preface
Midi 40 CO2 Incubator i
Models covered by this manual:
Catalog
Number
Manufacturer
Model Number Sensor Capacity (cu ft) Voltage
RMI300S-9-A 3403 T/C 1.4 115V/60Hz
RMI300S-9-V 3404 T/C 1.4 208/230V
50/60Hz

Thermo Scientificii Midi 40 CO2 Incubator
Preface
Contains Parts and Assemblies
Susceptible to Damage by
Electrostatic Discharge (ESD)
CAUTION
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s
Warning All internal adjustments and maintenance must be performed by qualified service personnel. s
• Use this product only in the way described in the product literature and in this manual. Before
using it, verify that this product is suitable for the intended use.
• Do not modify system components, especially the controller. Use OEM exact replacement
equipment or parts. Before use, confirm that the product has not been altered in any way.
• Disconnect the unit from all power sources before cleaning, troubleshooting, or performing
other maintenance on the product or its controls. To disconnect power supply to the incubator,
unplug the supply cord at the back of the incubator. Note that turning the key switch on the
front control panel to the Off position is not sufficient to disconnect power.
Warning The user is responsible for carrying out appropriate decontamination procedures when hazardous
materials are spilled on or inside the incubator. s
Material in this manual is for information purposes only. The contents and the product it describes are subject
to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related
to the use of this manual.
©2009 Thermo Fisher Scientific. All rights reserved.

Thermo Scientific Midi 40 CO2 Incubator iii
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Asphyxiation Hazard Warning. High concentrations of CO2can displace oxygen and cause
asphyxiation!
WEEE Compliance: Thermo Fisher Scientific has contracted with companies for recycling/disposal in
each EU Member State. For further information, send an email to [email protected].
4Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4Always dissipate extreme cold or heat and wear protective clothing.
4Always follow good hygiene practices.
4Each individual is responsible for his or her own safety.

Thermo Scientificiv Midi 40 CO2 Incubator
Preface
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International customers, please contact your local Thermo Scientific distributor.

Midi 40 CO2 Incubator vThermo Scientific
Table of Contents
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
Operating Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Pre-Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Set-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
Gas Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
Electrical Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
CO2Gas Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3
Component Locations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-4
Initial Start-Up Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Power / Key Switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Setpoint Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Key Switch and Control Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Setpoint and Alarm Programming . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
Service Mode Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
Restore Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
Calibration (Optional) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Access Program or Service Menu . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Temperature Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-5
CO2 Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-6
Control Systems Theory . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Overtemperature Monitoring System . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Door Heat System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
CO2Control System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
CO2Recovery (after door opening) . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
Section 1
Section 2
Section 3
Section 4
Section 5
Section 6

vi Midi 40 CO2 Incubator Thermo Scientific
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1
Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-2
CO2Filter Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-2
Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-3
Door Seal Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-3
Parts List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1
Warranty Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1
Table of Contents
Section 8
Section 7
Section 9

Midi 40 CO2 Incubator 1-1Thermo Scientific
Requirements
Section 1 Introduction
These incubators are designed to create a stable, reliable environment for
cell culture applications. They operate at temperatures ranging from 5°C
above ambient temperature to +60°C, accurate to ±0.2°C. The gas system
controls within ±0.1% of measurable setpoint.
• Thermal conductivity sensor provides stable, automatic injection of
CO2.
• Automatic Gas Shutoff control turns off the gas supply when the inner
glass door is opened to prevent wasting the control gas.
• Alarms:
– High and low level CO2setpoint alarms, adjustable.
– High and low temperature alarms; high alarm can be set by user.
– Battery Low alarm; control system has tested the alarm battery and
determined that it should be replaced.
Caution The function of the alarm battery is to operate the display and
alarm system ONLY during a power loss, while the key switch is in the
On or Alarm position. This battery system will not power the heaters, fan
motor or gas control system. Only qualified service technicians should
replace this battery. Replacement instructions and batteries are available
from Thermo Scientific. s
• A high quality two-stage, low pressure 15 psig (1.056 kg/cm2) pressure
regulator is required for proper operation of the CO2gas supply.
• In-line gas supply filters must be used on the CO2supply to prevent
damage to the solenoid valve(s):
– Type: Microbiological
– Specification: 0.3 micron
– Location: rear of unit
• CO2used in the incubator must be at least 99.9% pure.

1-2 Midi 40 CO2 Incubator Thermo Scientific
Section 1
Introduction
Specifications Technical Specifications Model 3403 Model 3404
Dimensions
Exterior (w x h x d) inch/cm 23.5 x 18 x 18.5 (59.7 x 45.7 x 47)
Interior (w x h x d) inch/cm 14 x 14 x 12 (35.5 x 35.5 x 30.5)
Construction
Cabinet configuration Benchtop
Interior non-corrosive stainless steel, type 304, mirror finish
Interior volume
Chamber capacity 1.4 cu ft (39.6 l)
Shelves 4
Construction stainless steel, perforated
Shelf dimensions (w x d ) inch/cm 13.5” x 11.5” (34.3 x 29.2)
Surface area per shelf 1.08 sq ft (0.1 sq m)
Temperature
Range 5°C above ambient to 60°C
Control ±0.1°C
Uniformity ±0.5°C @ 37°C
Alarm user programmable high/low
CO2
Range 0-20%
Control ±0.1%
Sensor long life thermal conductivity sensor
Uniformity 0.10%
Inlet pressure required 15 PSIG (1.0 bar), 99.5 medicinal grade CO2
Inlet air filtration 0.3 micron anti-microbial filter
Alarm user programmable high/low
Humidity
rH to 95% @ 37°C
Source humidity pan, removable
Electrical
Rated voltage 115V, 60Hz 208/230V, 50/60Hz
Amps/breaker 3A/15 2A/15
Weight
Net 60 lbs. (27kg)
Shipping 70 lbs. (32kg)
Warranty 1 year
All specifications are subject to change without notice.

Midi 40 CO2 Incubator 2-1Thermo Scientific
Section 2 Operating Standards
The incubators described in this manual are classified for use as stationary
equipment in a Pollution Degree 2 and Overvoltage Category II
environment, according to the UL 61010-1, 3rd Edition, 2012-05-11,
CAN/CSA-C22.2 No 61010-1 3rd Edition 2012-11, IEC/EN 61010-
1:2010, 3rd Edition, IEC/EN 61010-1-010: 2014, 3rd Edition.
These units are designed to operate under the following environmental
conditions:
• Indoor use only
• Altitude up to 2000m
• Maximum relative humidity 80% for temperatures up to 31°C
• Main supply voltage fluctuations not to exceed 10% of the nominal
voltage.

Midi 40 CO2 Incubator 3-1Thermo Scientific
Section 3 Pre-Installation
The following items are packaged and shipped inside the incubator
cabinet:
• This manual
• Shelves and shelf brackets
• 1/4 in. (6.35 mm) ID clear tubing for the gas connection
• Cordset
• 5” round humidity pan
• Additional CO2sensor gasket
• CO2disposable filter 99.97
Figure 3-1. Included Components (shelves and brackets not shown)
Cordset
Additional
gasket
Humidity
pan
Tubing
Filter
Operating
manual

3-2 Midi 40 CO2 Incubator Thermo Scientific
Section 3
Pre-Installation
Gas Supplies
Remove shelves from the inside of the incubator and clean the chamber.
Shelves and brackets can be autoclaved.
This unit weighs 60 lbs. (27kg). Be safe when moving it. Request
assistance when required, or use a lift truck.
Install the unit in a level area free from vibration with a minimum of three
inches (7.6 cm) of space on the sides and rear, and twelve inches (30.5 cm)
at the top. The floor must be able to support 40 PSI (single chamber
incubator) or 75 PSI (stack of two incubators with the addition of stacking
hardware kit).
Be sure to position the incubator so that the power cord (mains disconnect)
is easily accessible to disconnect power.
Do not position the equipment in direct sunlight or near any HVAC
duct/diffusers. The ambient temperature range at the work location must
be 59 to 90°F (15 to 32°C).
Verify that the incubator CO2gas supplies are available near the
installation area. The required gas supply pressure is 15 PSI, controlled by
a high-quality, two-stage regulator suitable for the connected input gas.
Warning Do not connect the gas at this time. s
Caution Connect the equipment to the correct power source. Be sure to
operate the incubator at the voltage specified on the dataplate. Incorrect
voltage can result in severe damage to the equipment. s
Warning For personal safety and trouble-free operation, this unit must be
properly grounded before it is used. Failure to ground the equipment may
cause personal injury or damage to the equipment. Always conform to the
National Electrical Code and local codes. Do not connect the unit to
overloaded power lines. s
The incubators described in this manual are available with the following
voltages: 115VAC, single-phase, 60Hz, 3A and 208/230VAC, single
phase, 50/60Hz, 2A. Be sure to check your dataplate to verify the correct
voltage for your unit.
Always connect the unit to a dedicated (separate) circuit. Do not exceed
the electrical and temperature ratings printed on the dataplate located near
the upper hinge of the unit. Electrical codes require fuse or circuit breaker
protection for branch circuit conductors. Use time delay fuses for #12
AWG circuits.
Electrical Connection
Set-Up
Location

Midi 40 CO2 Incubator 3-3Thermo Scientific
Section 3
Pre-Installation
CO2gas connection(s) are located at the rear of the unit.
Note The required gas supply pressure is 15 PSI. Make sure the supply is
available in the installation area. s
To install the CO2connections, complete the following steps:
1. Locate the included 1/4 in. (6.3mm) interior diameter tubing.
2. Cut a small length (approximately 6 in. [15cm]) from the roll of 1/4
in. (6.3mm) ID tubing.
3. Pull the cap off the gas connection port at the rear of the incubator
and attach one end of the tubing to the gas connection port.
4. Connect the opposite end of the tubing to a high-quality input gas
filter (0.3 micron supplied). Note that the side of the filter marked
“IN” faces the incoming gas connection, not the back of the incubator
(refer to Figure 1-2).
Figure 3-2. CO2Gas Connections
5. Use the remaining 1/4 in. ID tubing to connect the input gas to the
gas filter. Keep the tubing length as short as possible to minimize the
pressure drop.
Caution DO NOT turn on the gas supply at this time. s
CO2Gas Connections
1/4 in. ID Tubing
Gas Filter

3-4 Midi 40 CO2 Incubator Thermo Scientific
Section 3
Pre-Installation
Component Locations
humidity pan
Upper back
of unit
Inside
Chamber
Lower back
of unit
Power
inlet
Sample Port
CO2 inlet
sensor
dataplate
on left side

Midi 40 CO2 Incubator 4-1Thermo Scientific
Section 4 Initial Start-Up Procedures
On first start-up of the incubator, the control display will go through
several prompts to guide you through the entire set-up process, from
leveling the incubator to adjusting the operating parameters.
During normal operation, the control panel
display looks like Figure 4-1. The next section
explains all the control functions available
during normal operation. On initial start-up,
however, a series of prompts displays. This
section explains the start-up prompts and the
procedures to follow.
Turn the key switch (shown in Figure 4-1) to
the “1” position. Press and hold the up arrow
button to initiate the special start-up prompts. If
a temperature displays instead of a start-up
prompt, the up arrow button hasn’t been held
long enough. Turn the key switch back to “O”
and try again.
A prompt displays to accept or adjust the values
of several operating parameters. In each case,
press the up or down arrow buttons (increase or
decrease) to change a displayed value. Press
Mode when the displayed value is the one
desired. The modifiable value is always the one
flashing.
If a displayed parameter is not adjusted and 60 seconds elapse, the software
registers the existing default value and moves to the next parameter.
If no changes are needed to the factory-set defaults, press Mode repeatedly
until the end of the prompts.
Power / Key Switch
Setpoint Parameters
TEMP (C)
CO2 (%)
37.0
5.0
OI
Figure 4-1. Control Panel
key switch

4-2 Midi 40 CO2 Incubator Thermo Scientific
Section 4
Initial Start-Up Procedures
The adjustable parameters are:
• Temperature (default 37°C)
• Warm alarm (default 40°C; cannot deviate from the temperature
setpoint by less than 0.3°C)
• Cold alarm (default 34°C; cannot deviate from the temperature
setpoint by less than 0.3°C)
• CO2(default 5%)
• High CO2alarm (default 6%; cannot deviate from the CO2setpoint
by less than 1%)
• Low CO2alarm (default 4%; cannot deviate from the CO2setpoint by
less than 1%)
When parameter adjustments are complete, the screen returns to the
normal display (Figure 4-1). An additional message indicates that the
stabilization period has begun.
During this initial stabilization period, leave the door closed.
Setpoint Parameters
(continued)

Section 5 Key Switch and Control
Operation
To operate the incubator with alarms activated, be sure to turn the key
switch all the way to the rightmost position (as shown in Figure 5-1). To
avoid nuisance alarms, work with the key switch in the “I” position during
the set-up procedures described in
Section 4.
The control panel is located on the
front of your incubator.
Before loading and using your
incubator, be sure to take some time
to review the control panel functions.
The main display, during normal
operation, shows: cabinet
temperature in degrees Celsius, as
measured by the sensor inside the
cabinet; and percentage levels of
carbon dioxide (CO2). When one or
more of these fields flashes during
normal operation, an alarm or error
condition is indicated. Note that
alarm conditions are displayed only
when the key switch is turned to the
alarm position.
Midi 40 CO2 Incubator 5-1Thermo Scientific
TEMP (C)
CO2 (%)
37.0
5.0
OI
Figure 5-1. Key Switch
UP ARROW (INCREASE)
MODE
KEY SWITCH
DOWN ARROW (DECREASE)

In programming mode (described in detail in Table
5-1) the main display changes to show the setpoint
being viewed and related alarm setpoints. For example
(Figure 5-2):
The first value displayed is the temperature setpoint;
the next two values are the high temp alarm and low
temp alarm setpoints. The flashing value is one that
can be changed, using the up and down arrow buttons.
The up and down arrow buttons are used to change
setpoint values in programming mode and for various display functions.
The Mode pushbutton is used: to silence an audible alarm; to enter
programming and service modes; and in combination with the other
buttons for various display functions.
For full descriptions of display, programming, and service functions, refer
to Tables 5-1, 5-2, and 5-3.
5-2 Midi 40 CO2 Incubator Thermo Scientific
Section 5
Key Switch and Control Operation
Figure 5-2.
Programming Mode
Table 5-1. Control Panel Display
Set Temp
SETPOINT:
37.0C
40.2C
33.8C
Function Meaning Sequence Display
Normal operation Default display while incubator is running — Display shows cabinet temperature, CO2, Relative
Humidity.
Cold excursion Show coldest cabinet temperature since
last startup or reset Press Display shows cold excursion while button is pressed.
Warm excursion Show warmest cabinet temperature since
last startup or reset Press Display shows warm excursion while button is pressed.
Mode Silence audible alarm Press Mode Display shows current values, alarm or error field
continues to flash.
Reset Return to default display after 
excursion or alarm condition Press and
simultaneously Excursion values are reset; display shows current values. Display flashes twice

Midi 40 CO2 Incubator 5-3Thermo Scientific
Section 5
Key Switch and Control Operation
To enter Program mode, press and hold the MODE button until the
display indicates it is changing to Program mode. Press Mode repeatedly
to scroll through the available functions. Exit Program mode by scrolling
through all available functions and parameters using the Mode button, or
the display will automatically return to normal operating mode 30 seconds
after the last key entry.
Service mode can be entered from Program mode by pressing Mode and
holding for 5 seconds.Pressing Mode repeatedly scrolls through the
available functions. For any modifiable parameter, the up and down arrow
buttons can be used to adjust the value. The display automatically returns
to normal operating mode 30 seconds after the last key entry, or after
scrolling through all available functions and parameters.
When the incubator is first powered up, all factory-set program parameter
defaults can be restored as follows:
Table 5-2. Setpoint and Alarm Programming Functions
Function Programming Sequence
Adjust temperature setpoint Enter programming mode by pressing Mode and holding for 5 seconds. On release, the current temperature
setpoint value flashes in the display; use and to adjust it.
Adjust warm alarm setpoint Press Mode again. The current warm alarm setpoint value then flashes in the display; use and to adjust it.
There is a minimum deviation of 0.3º C between each alarm setpoint and the temperature setpoint.
Adjust cold alarm setpoint Press Mode again. The current cold alarm setpoint value then flashes in the display; use and to adjust it.
There is a minimum deviation of 0.3º C between each alarm setpoint and the temperature setpoint.
Adjust CO2setpoint Press Mode again. The display changes to display CO2setpoint and high and low CO2alarm setpoints, with the
operating CO2setpoint flashing. Use and to adjust it. There is a minimum deviation of 1% between each
alarm setpoint and the operating CO2 setpoint.
Adjust CO2high alarm setpoint Press Mode again. The current high CO2alarm setpoint then flashes; Use and to adjust it. There is a
minimum deviation of 1% between each alarm setpoint and the operating CO2 setpoint.
Adjust CO2low alarm setpoint Press Mode again. The current high CO2low setpoint then flashes; Use and to adjust it. There is a minimum
deviation of 1% between each alarm setpoint and the operating CO2 setpoint.
Set Temp offset Press Mode again. The display changes to display temperature offset (for calibration purposes). Use and to
adjust it.
Set CO2offset Press Mode again. The display changes to display CO2 offset (for calibration purposes). Use and to adjust it.
Table 5-3. Service Parameters
Table 5-4. Restore Defaults
Setpoint and Alarm
Programming
Service Mode
Parameters
Restore Function
Function Sequence Notes
Restore program defaults Press and simultaneously,
hold for five seconds Restores original (factory-set) values of all program parameters.
Parameter Notes
SN Serial number.
Check Sum Checksum to identify firmware version
PWM Info CO2 sensor and excursion information