Thermo Scientific RMI300S-9-A Operating instructions

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User Manual
Midi 40 Series
CO2Incubator
Operating and Maintenance Manual 7003403 Rev. 9

Thermo Scientific
MANUAL NUMBER 7003403
9 41116/IN-4785 11/30/16
Replaced Sample Port and CO2 Inlet labels on pg 3-4
ccs
8 40639 3/22/16 Updated to UL requirements, added risk assessments ccs
7 30073/IN-4491 7/7/15 Added stacking hardware kit to Parts List ccs
6 30073/IN-4491 7/28/14 Clarified specs in Location for stacking ccs
5 26439/IN-4050 2/5/13 Updated uniformity in Specifications ccs
4 28251/IN-4219 8/30/12 Removed RS-485 ccs
3 28728 6/18/12 Updated Calibration sections - pg 6-4 through 6-6 ccs
2 27796/IN-4127 10/3/11 Tubing size from 3/16 to 1/4” - pgs 4-1 and 4-3 ccs
-- -- 6/10/10 Corrected typo in Table 6-1 on pg 6-2 - removed O2 (per D. Wernerspach) ccs
Preface
Midi 40 CO2 Incubator i
Models covered by this manual:
Catalog
Number
Manufacturer
Model Number Sensor Capacity (cu ft) Voltage
RMI300S-9-A 3403 T/C 1.4 115V/60Hz
RMI300S-9-V 3404 T/C 1.4 208/230V
50/60Hz

Thermo Scientificii Midi 40 CO2 Incubator
Preface
Contains Parts and Assemblies
Susceptible to Damage by
Electrostatic Discharge (ESD)
CAUTION
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s
Warning All internal adjustments and maintenance must be performed by qualified service personnel. s
• Use this product only in the way described in the product literature and in this manual. Before
using it, verify that this product is suitable for the intended use.
• Do not modify system components, especially the controller. Use OEM exact replacement
equipment or parts. Before use, confirm that the product has not been altered in any way.
• Disconnect the unit from all power sources before cleaning, troubleshooting, or performing
other maintenance on the product or its controls. To disconnect power supply to the incubator,
unplug the supply cord at the back of the incubator. Note that turning the key switch on the
front control panel to the Off position is not sufficient to disconnect power.
Warning The user is responsible for carrying out appropriate decontamination procedures when hazardous
materials are spilled on or inside the incubator. s
Material in this manual is for information purposes only. The contents and the product it describes are subject
to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related
to the use of this manual.
©2009 Thermo Fisher Scientific. All rights reserved.

Thermo Scientific Midi 40 CO2 Incubator iii
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Asphyxiation Hazard Warning. High concentrations of CO2can displace oxygen and cause
asphyxiation!
WEEE Compliance: Thermo Fisher Scientific has contracted with companies for recycling/disposal in
each EU Member State. For further information, send an email to [email protected].
4Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4Always dissipate extreme cold or heat and wear protective clothing.
4Always follow good hygiene practices.
4Each individual is responsible for his or her own safety.

Thermo Scientificiv Midi 40 CO2 Incubator
Preface
Do You Need Information or Assistance on
Thermo Scientific Products?
If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:
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Our staff can supply technical information about proper setup, operation orService Support
troubleshooting of your equipment. We can fill your needs for spare or replacement parts or
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locate the problem and, chances are, correct it yourself...over the telephone without a service
call.
When more extensive service is necessary, we will assist you with direct factory trained
technicians or a qualified service organization for on-the-spot repair. If your service need is
covered by the warranty, we will arrange for the unit to be repaired at our expense and to your
satisfaction.
Regardless of your needs, our professional telephone technicians are available to assist you
Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by
telephone or fax. If you wish to write, our mailing address is:
Thermo Fisher Scientific (Asheville) LLC
401 Millcreek Road, Box 649
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International customers, please contact your local Thermo Scientific distributor.

Midi 40 CO2 Incubator vThermo Scientific
Table of Contents
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
Operating Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Pre-Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Set-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
Gas Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
Electrical Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
CO2Gas Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3
Component Locations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-4
Initial Start-Up Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Power / Key Switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Setpoint Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Key Switch and Control Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Setpoint and Alarm Programming . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
Service Mode Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
Restore Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
Calibration (Optional) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Access Program or Service Menu . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Temperature Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-5
CO2 Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-6
Control Systems Theory . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Overtemperature Monitoring System . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Door Heat System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
CO2Control System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
CO2Recovery (after door opening) . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
Section 1
Section 2
Section 3
Section 4
Section 5
Section 6

vi Midi 40 CO2 Incubator Thermo Scientific
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1
Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-2
CO2Filter Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-2
Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-3
Door Seal Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-3
Parts List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1
Warranty Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1
Table of Contents
Section 8
Section 7
Section 9

Midi 40 CO2 Incubator 1-1Thermo Scientific
Requirements
Section 1 Introduction
These incubators are designed to create a stable, reliable environment for
cell culture applications. They operate at temperatures ranging from 5°C
above ambient temperature to +60°C, accurate to ±0.2°C. The gas system
controls within ±0.1% of measurable setpoint.
• Thermal conductivity sensor provides stable, automatic injection of
CO2.
• Automatic Gas Shutoff control turns off the gas supply when the inner
glass door is opened to prevent wasting the control gas.
• Alarms:
– High and low level CO2setpoint alarms, adjustable.
– High and low temperature alarms; high alarm can be set by user.
– Battery Low alarm; control system has tested the alarm battery and
determined that it should be replaced.
Caution The function of the alarm battery is to operate the display and
alarm system ONLY during a power loss, while the key switch is in the
On or Alarm position. This battery system will not power the heaters, fan
motor or gas control system. Only qualified service technicians should
replace this battery. Replacement instructions and batteries are available
from Thermo Scientific. s
• A high quality two-stage, low pressure 15 psig (1.056 kg/cm2) pressure
regulator is required for proper operation of the CO2gas supply.
• In-line gas supply filters must be used on the CO2supply to prevent
damage to the solenoid valve(s):
– Type: Microbiological
– Specification: 0.3 micron
– Location: rear of unit
• CO2used in the incubator must be at least 99.9% pure.

1-2 Midi 40 CO2 Incubator Thermo Scientific
Section 1
Introduction
Specifications Technical Specifications Model 3403 Model 3404
Dimensions
Exterior (w x h x d) inch/cm 23.5 x 18 x 18.5 (59.7 x 45.7 x 47)
Interior (w x h x d) inch/cm 14 x 14 x 12 (35.5 x 35.5 x 30.5)
Construction
Cabinet configuration Benchtop
Interior non-corrosive stainless steel, type 304, mirror finish
Interior volume
Chamber capacity 1.4 cu ft (39.6 l)
Shelves 4
Construction stainless steel, perforated
Shelf dimensions (w x d ) inch/cm 13.5” x 11.5” (34.3 x 29.2)
Surface area per shelf 1.08 sq ft (0.1 sq m)
Temperature
Range 5°C above ambient to 60°C
Control ±0.1°C
Uniformity ±0.5°C @ 37°C
Alarm user programmable high/low
CO2
Range 0-20%
Control ±0.1%
Sensor long life thermal conductivity sensor
Uniformity 0.10%
Inlet pressure required 15 PSIG (1.0 bar), 99.5 medicinal grade CO2
Inlet air filtration 0.3 micron anti-microbial filter
Alarm user programmable high/low
Humidity
rH to 95% @ 37°C
Source humidity pan, removable
Electrical
Rated voltage 115V, 60Hz 208/230V, 50/60Hz
Amps/breaker 3A/15 2A/15
Weight
Net 60 lbs. (27kg)
Shipping 70 lbs. (32kg)
Warranty 1 year
All specifications are subject to change without notice.

Midi 40 CO2 Incubator 2-1Thermo Scientific
Section 2 Operating Standards
The incubators described in this manual are classified for use as stationary
equipment in a Pollution Degree 2 and Overvoltage Category II
environment, according to the UL 61010-1, 3rd Edition, 2012-05-11,
CAN/CSA-C22.2 No 61010-1 3rd Edition 2012-11, IEC/EN 61010-
1:2010, 3rd Edition, IEC/EN 61010-1-010: 2014, 3rd Edition.
These units are designed to operate under the following environmental
conditions:
• Indoor use only
• Altitude up to 2000m
• Maximum relative humidity 80% for temperatures up to 31°C
• Main supply voltage fluctuations not to exceed 10% of the nominal
voltage.