Thermo Scientific 3010 Operating instructions

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User Manual
Model 3010 and 3020
Water Jacketed CO2 Incubators
Operating and Maintenance Manual 7013010 Rev. 2

Thermo Scientific
MANUAL NUMBER 7013010
2 40087/IN-4743 7/27/16 Changed label on remote alarm artwork to note - pg 5-1 ccs
1 40639 5/3/16 Added risk assessment info ccs
0 27988/IN-4478 4/7/14 Release 2 - Original ccs
REV ECR/ECN DATE DESCRIPTION By
Preface
Water Jacketed CO2 Incubator i
Models covered:
Model Sensor* Voltage**
3010 T/C sensor 115V
3020 IR sensor 115V
*T/C is a thermal conductivity sensor, IR is an infra-red sensor.
**All units are 50/60Hz.

Thermo Scientificii Water Jacketed CO2 Incubator
Preface
Contains Parts and Assemblies
Susceptible to Damage by
Electrostatic Discharge (ESD)
CAUTION
Important Read this operating manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s
Warning All internal adjustments and maintenance must be performed by qualified service personnel. s
Material in this manual is for information purposes only. The contents and the product it describes are subject
to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to
the use of this manual.
Intended Use
The Thermo Scientific Water Jacketed CO2incubators are intended to provide an environment with con-
trolled temperature and Carbon Dioxide (CO2) as well as elevated humidity (RH) for the growth of cultivat-
ing cell and tissue cultures.
Non Intended Use
The incubators are not intended for use by the general public. The units are not intended for use within the
patient environment or outdoors. These units are not designed to be used with liquid CO2 or liquid N2.
These units should not be used with any flammable or toxic materials.
©2014 Thermo Fisher Scientific. All rights reserved.

Thermo Scientific Water Jacketed CO2 Incubator iii
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible
injury.
Asphyxiation Hazard Warning. High concentrations of CO2can displace oxygen and cause
asphyxiation!
Lifting Hazard Warning. The incubator weighs more than 200 lbs (91kgs). Take adequate safety
measures when moving this device.
WEEE Compliance: Thermo Fisher Scientific has contracted with companies for recycling/disposal
in each EU Member State. For further information, send an email to
[email protected].
4Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4Always dissipate extreme cold or heat and wear protective clothing.
4Always follow good hygiene practices.
4Each individual is responsible for his or her own safety.

Thermo Scientificiv Water Jacketed CO2 Incubator
Preface
Do You Need Information or Assistance on
Thermo Scientific Products?
If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:
1-740-373-4763 Direct
1-800-438-4851 Toll Free, U.S. and Canada
1-877-213-8051 FAX
http://www.thermofisher.com Internet Worldwide Web Home Page
[e-mail] Tech Support Email Address
Certified Service Web Pagewww.unitylabservices.com
Our staff can provide information on pricing and give you quotations. We canSales Support
take your order and provide delivery information on major equipment items or make
arrangements to have your local sales representative contact you. Our products are listed on the
Internet and we can be contacted through our Internet home page.
Our staff can supply technical information about proper setup, operation orService Support
troubleshooting of your equipment. We can fill your needs for spare or replacement parts or
provide you with on-site service. We can also provide you with a quotation on our Extended
Warranty for your Thermo Scientific products.
Whatever Thermo Scientific products you need or use, we will be happy to discuss your
applications. If you are experiencing technical problems, working together, we will help you
locate the problem and, chances are, correct it yourself...over the telephone without a service
call.
When more extensive service is necessary, we will assist you with direct factory trained
technicians or a qualified service organization for on-the-spot repair. If your service need is
covered by the warranty, we will arrange for the unit to be repaired at our expense and to your
satisfaction.
Regardless of your needs, our professional telephone technicians are available to assist you
Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by
telephone or fax. If you wish to write, our mailing address is:
Thermo Fisher Scientific (Asheville) LLC
401 Millcreek Road, Box 649
Marietta, OH 45750
International customers, please contact your local Thermo Scientific distributor.

Thermo Scientific Water Jacketed CO2 Incubator v
Preface
Warranty Notes
Information You Should Know Before Requesting Warranty Service
•Locate themodel and serial numbers.Aserial tag is located on the unit itself.
•For equipment service or maintenance, or with technical or special application inquiries, contact Technical
Services at 1-800-438-4851 or 1-740-373-4763 (USA and Canada).Outside the USA, contact your local
distributor.
Repairs NOT Covered Under Warranty
•Calibration of control parameters.Nominal calibrations are performed at the factory; typically ±1°Cfor
temperature, ±1% for gases, and ±5% for humidity. Our service personnel can provide precise calibrations as
abillable service at your location. Calibration after awarranty repair is covered under the warranty.
•Damage resulting from use of improper quality water, chemicals or cleaning agents detrimental to
equipment materials.
•Service calls for improper installation or operating instructions. Corrections to any of the following
are billable services:
1)electrical service connection
2)tubing connections
3)gas regulators
4)gas tanks
5)unit leveling
6)room ventilation
7)adverse ambient temperature fluctuations
8)any repair external to the unit
•Damage resulting from accident, alteration, misuse, abuse, fire, flood, acts of God, or improper
installation.
•Repairs to parts or systems resulting from unauthorized unit modifications.
•Any labor costs other than that specified during theparts and labor warranty period, which may
include additional warranty on CO2sensors, blower motors, water jackets, etc.

Water Jacketed CO2 Incubator viThermo Scientific
Table ofContents
Installation and Start-Up ......................................1-1
Control Panel Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
Control Panel Keys, Displays & Indicators . . . . . . . . . . . . . . . . . . . .1-2
Unit Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Stack the Incubators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Preliminary Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5
Shelf Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Air Sample Filter Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Access Port Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Connect Unit to Electrical Power . . . . . . . . . . . . . . . . . . . . . . . . . . .1-7
Level the Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-7
Fill the Humidity Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-8
Fill the Water Jacket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-8
Connect the CO2Gas Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-9
Incubator Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-10
Set the Operating Temperature Setpoint . . . . . . . . . . . . . . . . . . .1-10
Set the Overtemp Setpoint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-11
Set the CO2 Setpoint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-12
Calibration ..................................................2-1
Calibrate Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Calibrate Thermal Conductivity (T/C) CO2System . . . . . . . . . . . . .2-2
Calibrate Infra-Red (IR) CO2System . . . . . . . . . . . . . . . . . . . . . . . .2-3
Alarms ......................................................3-1
Section 1
Section 2
Section 3

vii Water Jacketed CO2 Incubator Thermo Scientific
Routine Maintenance .........................................4-1
Clean the Incubator Interior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Reverse the Door Swing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-4
Reverse the Hinges for Exterior Door . . . . . . . . . . . . . . . . . . . . . . .4-4
Clean the Humidity Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-4
Clean the Cabinet Exterior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-4
Replace Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-10
Replace the Access Port Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-11
Replace the Air Sample Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-11
Add or Replenish Rust Inhibitor . . . . . . . . . . . . . . . . . . . . . . . . . . .4-12
Drain Water Jacket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-12
Remote Alarms ...............................................5-1
Specifications ...............................................6-1
Parts List ....................................................7-1
Electrical Schematics ........................................8-1
Warranty Information .........................................9-1
Table of Contents
Section 5
Section 6
Section 8
Section 7
Section 9
Section 4

Water Jacketed CO2 Incubator 1-1Thermo Scientific
Section 1 Installation and Start-Up
• Outer Door - Reversible to opposite swing, see Section 4.
• Heated Inner Door - Keeps chamber interior dry. Reversible to opposite
swing, see Section 4.
• Chamber Gas Sample Port - Used for sampling chamber CO2content,
for calibration (Section 2).
• Main Power Switch
• Control Panel - Keypad, Displays and Indicators. (See Figure 1-2).
• Fill Port - Used for filling the water jacket.
• Water Jacket Vent - Allows air to escape from the water jacket during
filling and normal expansion and contraction when the incubator heats
or cools.
• Leveling Legs - Used to level the unit.
• Water Jacket Drain – Remove plug and use hose barb insert included.
Plug when not in use.
Note The incubators are stackable. Information follows in this section. s
*When setting up the incubator, install the cover plate packed inside the
incubator shipping carton.
Heated Glass
Inner Door
Leveling
Legs (4)
Jacket
Vent
Water Jacket
Fill Port
Jacket
Drain
(removeplug before using)*
Control Panel
Chamber Gas
SamplePort
Power
Switch
Hose
Barb
Insert
OverTemp
LowTemp
HighCO2
LowCO2
AddWater
DoorOpen
MODE
SILENCE
Run
SetTemp
SetOtemp
SetCO2
CalTemp
CalCO2
ENTER
Inject
Heat Temp °C
%CO2
Figure 1-1.Water Jacket Incubator