Thermo Scientific 3010 Operating instructions

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User Manual
Model 3010 and 3020
Water Jacketed CO2 Incubators
Operating and Maintenance Manual 7013010 Rev. 2

Thermo Scientific
MANUAL NUMBER 7013010
2 40087/IN-4743 7/27/16 Changed label on remote alarm artwork to note - pg 5-1 ccs
1 40639 5/3/16 Added risk assessment info ccs
0 27988/IN-4478 4/7/14 Release 2 - Original ccs
REV ECR/ECN DATE DESCRIPTION By
Preface
Water Jacketed CO2 Incubator i
Models covered:
Model Sensor* Voltage**
3010 T/C sensor 115V
3020 IR sensor 115V
*T/C is a thermal conductivity sensor, IR is an infra-red sensor.
**All units are 50/60Hz.

Thermo Scientificii Water Jacketed CO2 Incubator
Preface
Contains Parts and Assemblies
Susceptible to Damage by
Electrostatic Discharge (ESD)
CAUTION
Important Read this operating manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s
Warning All internal adjustments and maintenance must be performed by qualified service personnel. s
Material in this manual is for information purposes only. The contents and the product it describes are subject
to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to
the use of this manual.
Intended Use
The Thermo Scientific Water Jacketed CO2incubators are intended to provide an environment with con-
trolled temperature and Carbon Dioxide (CO2) as well as elevated humidity (RH) for the growth of cultivat-
ing cell and tissue cultures.
Non Intended Use
The incubators are not intended for use by the general public. The units are not intended for use within the
patient environment or outdoors. These units are not designed to be used with liquid CO2 or liquid N2.
These units should not be used with any flammable or toxic materials.
©2014 Thermo Fisher Scientific. All rights reserved.

Thermo Scientific Water Jacketed CO2 Incubator iii
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible
injury.
Asphyxiation Hazard Warning. High concentrations of CO2can displace oxygen and cause
asphyxiation!
Lifting Hazard Warning. The incubator weighs more than 200 lbs (91kgs). Take adequate safety
measures when moving this device.
WEEE Compliance: Thermo Fisher Scientific has contracted with companies for recycling/disposal
in each EU Member State. For further information, send an email to
[email protected].
4Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4Always dissipate extreme cold or heat and wear protective clothing.
4Always follow good hygiene practices.
4Each individual is responsible for his or her own safety.

Thermo Scientificiv Water Jacketed CO2 Incubator
Preface
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Thermo Scientific Products?
If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:
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Our staff can supply technical information about proper setup, operation orService Support
troubleshooting of your equipment. We can fill your needs for spare or replacement parts or
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Whatever Thermo Scientific products you need or use, we will be happy to discuss your
applications. If you are experiencing technical problems, working together, we will help you
locate the problem and, chances are, correct it yourself...over the telephone without a service
call.
When more extensive service is necessary, we will assist you with direct factory trained
technicians or a qualified service organization for on-the-spot repair. If your service need is
covered by the warranty, we will arrange for the unit to be repaired at our expense and to your
satisfaction.
Regardless of your needs, our professional telephone technicians are available to assist you
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telephone or fax. If you wish to write, our mailing address is:
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401 Millcreek Road, Box 649
Marietta, OH 45750
International customers, please contact your local Thermo Scientific distributor.

Thermo Scientific Water Jacketed CO2 Incubator v
Preface
Warranty Notes
Information You Should Know Before Requesting Warranty Service
•Locate themodel and serial numbers.Aserial tag is located on the unit itself.
•For equipment service or maintenance, or with technical or special application inquiries, contact Technical
Services at 1-800-438-4851 or 1-740-373-4763 (USA and Canada).Outside the USA, contact your local
distributor.
Repairs NOT Covered Under Warranty
•Calibration of control parameters.Nominal calibrations are performed at the factory; typically ±1°Cfor
temperature, ±1% for gases, and ±5% for humidity. Our service personnel can provide precise calibrations as
abillable service at your location. Calibration after awarranty repair is covered under the warranty.
•Damage resulting from use of improper quality water, chemicals or cleaning agents detrimental to
equipment materials.
•Service calls for improper installation or operating instructions. Corrections to any of the following
are billable services:
1)electrical service connection
2)tubing connections
3)gas regulators
4)gas tanks
5)unit leveling
6)room ventilation
7)adverse ambient temperature fluctuations
8)any repair external to the unit
•Damage resulting from accident, alteration, misuse, abuse, fire, flood, acts of God, or improper
installation.
•Repairs to parts or systems resulting from unauthorized unit modifications.
•Any labor costs other than that specified during theparts and labor warranty period, which may
include additional warranty on CO2sensors, blower motors, water jackets, etc.

Water Jacketed CO2 Incubator viThermo Scientific
Table ofContents
Installation and Start-Up ......................................1-1
Control Panel Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
Control Panel Keys, Displays & Indicators . . . . . . . . . . . . . . . . . . . .1-2
Unit Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Stack the Incubators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Preliminary Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5
Shelf Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Air Sample Filter Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Access Port Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Connect Unit to Electrical Power . . . . . . . . . . . . . . . . . . . . . . . . . . .1-7
Level the Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-7
Fill the Humidity Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-8
Fill the Water Jacket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-8
Connect the CO2Gas Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-9
Incubator Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-10
Set the Operating Temperature Setpoint . . . . . . . . . . . . . . . . . . .1-10
Set the Overtemp Setpoint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-11
Set the CO2 Setpoint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-12
Calibration ..................................................2-1
Calibrate Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Calibrate Thermal Conductivity (T/C) CO2System . . . . . . . . . . . . .2-2
Calibrate Infra-Red (IR) CO2System . . . . . . . . . . . . . . . . . . . . . . . .2-3
Alarms ......................................................3-1
Section 1
Section 2
Section 3

vii Water Jacketed CO2 Incubator Thermo Scientific
Routine Maintenance .........................................4-1
Clean the Incubator Interior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Reverse the Door Swing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-4
Reverse the Hinges for Exterior Door . . . . . . . . . . . . . . . . . . . . . . .4-4
Clean the Humidity Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-4
Clean the Cabinet Exterior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-4
Replace Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-10
Replace the Access Port Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-11
Replace the Air Sample Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-11
Add or Replenish Rust Inhibitor . . . . . . . . . . . . . . . . . . . . . . . . . . .4-12
Drain Water Jacket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-12
Remote Alarms ...............................................5-1
Specifications ...............................................6-1
Parts List ....................................................7-1
Electrical Schematics ........................................8-1
Warranty Information .........................................9-1
Table of Contents
Section 5
Section 6
Section 8
Section 7
Section 9
Section 4

Water Jacketed CO2 Incubator 1-1Thermo Scientific
Section 1 Installation and Start-Up
• Outer Door - Reversible to opposite swing, see Section 4.
• Heated Inner Door - Keeps chamber interior dry. Reversible to opposite
swing, see Section 4.
• Chamber Gas Sample Port - Used for sampling chamber CO2content,
for calibration (Section 2).
• Main Power Switch
• Control Panel - Keypad, Displays and Indicators. (See Figure 1-2).
• Fill Port - Used for filling the water jacket.
• Water Jacket Vent - Allows air to escape from the water jacket during
filling and normal expansion and contraction when the incubator heats
or cools.
• Leveling Legs - Used to level the unit.
• Water Jacket Drain – Remove plug and use hose barb insert included.
Plug when not in use.
Note The incubators are stackable. Information follows in this section. s
*When setting up the incubator, install the cover plate packed inside the
incubator shipping carton.
Heated Glass
Inner Door
Leveling
Legs (4)
Jacket
Vent
Water Jacket
Fill Port
Jacket
Drain
(removeplug before using)*
Control Panel
Chamber Gas
SamplePort
Power
Switch
Hose
Barb
Insert
OverTemp
LowTemp
HighCO2
LowCO2
AddWater
DoorOpen
MODE
SILENCE
Run
SetTemp
SetOtemp
SetCO2
CalTemp
CalCO2
ENTER
Inject
Heat Temp °C
%CO2
Figure 1-1.Water Jacket Incubator

1-2 Water Jacketed CO2 Incubator Thermo Scientific
Section 1
Installation and Start-Up
• SILENCE: Mutes the audible alarm. See Section 3 for ringback times.
• Alarm Indicators: Specific indicator flashes during an alarm condition.
• MODE: Switch used to select desired mode.
• Mode Indicators: Selected mode indicator lights.
• Up and Down Arrows: Increase or decrease parameter values, toggle
selected mode choices.
• Enter: Saves the value being changed to memory.
• Heat Indicator: Lights when power is applied to the heaters.
• Inject Indicator: Lights when gas is being injected into the incubator.
• Temp °C Display: Displays chamber temperature, temperature
setpoint or overtemperature setpoint
• % CO2 Display: Displays percentage of CO2in the chamber or CO2
setpoint
The Model 3010 water jacket incubator has six modes, which allow
incubator setup. The modes are as follows:
• Run is the default mode for the incubator during normal operation.
• Set Temp is used to enter system temperature setpoints for incubator
operation.
• Set Otemp is used to enter system overtemperature setpoints for
incubator operation.
• Set CO2 is used to enter system CO2setpoints for incubator
operation.
• Cal Temp is used to calibrate displayed chamber temperature (offset)
• Cal CO2 is used to calibrate displayed percentage of CO2in chamber
(offset).
Control Panel Keys,
Displays & Indicators
Over Temp
Low Temp
High CO2
Low CO2
Add Water
Door Open
MODE
SILENCE
Run
Set Temp
Set Otemp
Set CO2
Cal Temp
Cal CO2
ENTER
Inject
Heat Temp °C
% CO2
Alarm indicators Mode indicators
Up and down
arrow keys
Heat
indicator
Inject
indicator
Displays chamber temp,
temp setpoint, overtemp setpoint
Displays CO2% in chamber,
CO2 setpoint
Figure 1-2.Control Panel
Control Panel
Operation

Water Jacketed CO2 Incubator 1-3Thermo Scientific
Section 1
Installation and Start-Up
Stacking Incubators
1. Locate the unit on a firm, level surface capable of supporting the unit’s
operational weight of 365 lbs. (166kg) plus the estimated total sample
and user equipment weight.
2. Locate the incubator away from exterior doors and windows as changes
in outdoor temperatures and contact with direct sunlight can affect the
anti-condensation functions of the unit.
3. Position the door opening away from forced air heating and cooling
ducts as these can carry dust, dirt, and other contaminants into the
incubator chamber, as well as negatively affect the door opening
recovery time.
4. Allow enough clearance behind the unit for electrical and gas hook-up.
Warning This incubator weighs 265 lbs (120kg) before filling the water
jacket. If the unit has been in service, disconnect the power cord from
both the unit and the power source, turn off all gas regulators, and
disconnect all tubing and any other connections from the rear of the
electronics drawer before completely draining the water jacket. Have
sufficient personnel available to lift the unit into place using proper lifting
techniques according to local EHS policies. Lift from both sides of the
unit (left and right, not front and back) holding it from the bottom. Do
not lift the incubator by the outer door as this could damage the hinges
and/or the outer door may come loose. Note that the outer door can be
removed to reduce weight and make it easier to lift the unit by its corners.
Do not push or pull the incubator along the floor as this could damage the
leveling feet. s
Warning If the units have been in service, disconnect the power cord
connector and drain the water jacket of the designated top unit before
stacking. s
Note Stacking brackets (shown at left), washers, and bolts
for stacking are included with each unit. s
Figure 1-3.Stacking brackets
Unit Installation

1. Designate one incubator to be the top unit and the other as the
bottom unit. Remove the base cover plate from the top unit using the
finger holes in the base, or a slotted screwdriver (Figure 1-4).
2. Note the two slots in the base of the incubator that accommodate the
stacking bolts. Refer to Figure 1-5.
3. Remove the two plastic plugs from the bolt holes in the exterior top of
the bottom unit. Install the 1/2” long 5/16-18 stacking bolts and
washers into the bolt holes - do not tighten the bolts at this time.
Refer to Figure 1-6.
4. Unscrew and remove the leveling feet from the top unit and lift it onto
the bottom unit, off-setting the base of the top unit approximately 2-3
inches behind the stacking bolts and washers.
1-4 Water Jacketed CO2 Incubator Thermo Scientific
Section 1
Installation and Start-Up
Stacking Incubators
(continued)
Figure 1-6.Bolt Holes
Figure 1-7.Align Units
Figure 1-4.Remove Base Cover Plate
Figure 1-5.Stacking Bolt Slots
Warning This incubator weighs
265 lbs (120kg) before filling. Have
sufficient personnel to lift it. s
5. Align the sides of the top unit with the
bottom unit and slide the top unit forward
until the slots in the base of the top unit
align with the 5/16”-18 stacking bolts in
the exterior top of the bottom unit. Refer
to Figure 1-7.

6. Remove and save the two screws from the back of the control panel on
the bottom unit as shown in Figure 1-8.
7. Insert the stacking brackets into the slots on the rear of the control
panel of the bottom unit (Figure 1-5). Align the slots in the brackets
with the mounting holes on the rear of the incubators. Secure the
brackets with the screws saved above and the 1/4-20 bolts provided in
the stacking kit. A 7/16” wrench or socket will be required for the
bolts.
8. Secure the base of the top unit to the exterior top of the bottom unit
by tightening the 5/16-18 stacking bolts using a 1/2” (13mm) wrench
or suitable tool.
9. Replace the base cover on the top unit.
10. The stacked units are ready to be placed into service.
1. Remove the protective plastic coating on the shelf supports and air
duct, if present.
2. Using a suitable laboratory disinfectant, clean all interior surfaces
including shelves and shelf supports, door gaskets, blower wheel and
CO2sensor. See Section 5.
Caution Before using any cleaning or decontamination method except
those recommended by the manufacturer, users should check with the
manufacturer that the proposed method will not damage the equipment.
Accidental spills of hazardous materials on or inside this unit are the
responsibility of the user. s
Water Jacketed CO2 Incubator 1-5Thermo Scientific
Section 1
Installation and Start-Up
Stacking Incubators
(continued)
Figure 1-8.Remove and Save Screws
Preliminary Cleaning

Locate the opening in the top left corner of the interior chamber. Remove
the tape from the opening on the outside of the unit. Locate the stopper
with filter in the hardware bag. Install in the opening inside the chamber.
See Figure 1-9.
1. Remove the filter from the shipping bag.
2. Separate one section of the tubing from the filter. Install this section to
the fitting on the blower plate.
3. After installing the top duct, connect the filter assembly to the tubing
coming through the top duct.
4. Insert the free end of the air sample filter tubing into the larger hole in
the back of the blower scroll. See Figure 1-9 for completed
configuration.
1. Install the side ducts with the tabs
facing into the center of the chamber
with their slots up. There are no right
side or left side ducts, simply rotate one
of them to fit the opposite side. Tilt the
side ducts as they are placed in the
chamber so the tops fit into the top air
duct, then guide them into the vertical
position. Figure 1-10 shows the duct as
it would be oriented for the right side
of the chamber.
1-6 Water Jacketed CO2 Incubator Thermo Scientific
Section 1
Installation and Start-Up
Access Port
Installation
Temp
sensor
Access
port filter
Air sample
filter
Figure 1-9.Filters
Air Sample Filter
Installation
ShelfInstallation
Figure 1-10.Channels

2. Referring to Figure 1-10, note that there is no difference between left
and right side shelf channels.
3. Install the shelf channels by
placing the channel’s rear slot
over the appropriate rear tab on
the side duct. Pull the shelf
channel forward and engage the
channel’s front slot into the side
duct’s appropriate forward tab.
Refer to Figure 1-11.
4. Figure 1-12 shows one of the channels
installed on the right side duct.
Check the unit for being level by placing a
bubble-style level on one of the shelves.
Turn the hex nut on the leveler
counterclockwise to lengthen the leg, or
clockwise to shorten it. Level the unit front-
to-back and left-to-right.
See the serial tag on the side of the unit for electrical specifications, or refer
to the electrical schematics included in this manual.
Warning Connect the incubator to a grounded dedicated circuit only. The
power cord connector is the mains disconnect device for the incubator.
Position the unit so that it can be easily disconnected. s
Plug the provided power cord into the power inlet connector (Figure 1-13)
and into the grounded dedicated circuit.
Water Jacketed CO2 Incubator 1-7Thermo Scientific
Section 1
Installation and Start-Up
Level the Unit
ShelfInstallation
(continued)
Figure 1-11.Channel Tabs
Figure 1-12.Channel Installed
Connect Unit to
Electrical Power
CO2 inlet Power Cord
Connector
Remote
Alarm -
J
-11 telephone-style
connector
Figure 1-13.Rear Panel,Showing All Options

Turn the power switch on. ADD WATER will appear in the message
center. Press the Silence key to silence the alarm.
Note The fill port has a plug that must be removed before filling and
replaced after filling is complete.
Caution Chlorine is detrimental to stainless steel. Using chlorinated tap
water, or additives that contain chlorine, will void the water-jacket
warranty! s
Fill the water jacket with 11.7 gallons (43.5 liters) of pure water. Silicone
tubing and a funnel are shipped in the accessory bag with the unit.
For ease of connecting/disconnecting, use the silicone
tubing provided to connect directly to the fill port. See
Figure 1-1 and this detail. Do not install vinyl tubing
directly onto the fill port as it may be difficult to
remove. A 3/8” to 3/8” hose connector has been
provided to attach lengths of vinyl tubing to the silicone
fill port tubing, to reach the distilled water source.
When the jacket is full, the audible alarm sounds a continuous tone for 10
seconds and the alarm condition clears. Refer to Section 4, Table of
Alarms.
Note Model 3010 Water Jacket Incubator is shipped from the factory with
a rust inhibitor added to the water inside the unit. The rust inhibitor must
be replenished every 2 years. See Section 5 to drain the water jacket and for
the correct proportion of rust inhibitor to the water.
For best operation of the incubator, sterilized distilled, demineralized or
de-ionized water should be used in the humidity pan. Water purity should
be in the resistance range of 50K to 1M Ohm/cm, or a conductivity range
of 20.0 to 1.0 uS/cm. Refer to ASTM Standard D5391-93 or D4195-88
for measuring water purity.
Distillation systems, as well as some types of reverse osmosis water purity
systems, can produce water in the quality range specified. Tap water is not
recommended as it may contain chlorine, which can deteriorate the
stainless steel. Tap water may also have a high mineral content, which
would produce a build-up of scale in the pan. High purity or ultra pure
water is not recommended as it is an extremely aggressive solvent and will
deteriorate the stainless steel. High purity water has a resistance of above
1M to 18M Ohm.
1-8 Water Jacketed CO2 Incubator Thermo Scientific
Section 1
Installation and Start-Up
Fill the Water Jacket
Detail 1.Fill Port
Fill the Humidity Pan

Even high purity water can contain bacteria and organic contaminants.
Water should always be sterilized or treated with a decontaminant, safe for
use with stainless steel as well as safe for the product, prior to being
introduced into the humidity pan.
Caution Distilled or de-ionized water used in the humidity reservoir must
be within a water quality resistance range of 50K to 1M Ohm/cm to
protect and prolong the life of the stainless steel. Use of water outside the
specified range will decrease the operating life of the unit and void
warranty. s
Fill the humidity pan to within 1/2 inch of the top with sterile, distilled
water. Place the pan directly on the incubator floor to ensure optimum
humidity and temperature response.
For applications requiring higher humidity conditions, the pan should be
placed against the left side wall of the incubator. The ductwork has been
designed for this purpose. Also, the CO2sample port may be capped to
assist in achieving greater RH. In some ambients, this may cause
condensation to form in the chamber.
Check the level and change the water frequently to avoid contamination.
Do not allow the water level to fluctuate significantly. “Dry-outs” will have
an adverse effect on the humidity level, as well as CO2calibration.
Warning High concentrations of CO2gas can cause asphyxiation! OSHA
Standards specify that employee exposure to carbon dioxide in any eight-
hour shift of a 40-hour work week shall not exceed the eight-hour time
weighted average of 5000 PPM (0.5% CO2). The short term exposure
limit for 15 minutes or less is 30,000 PPM (3% CO2). Carbon dioxide
monitors are recommended for confined areas where concentrations of
carbon dioxide gas can accumulate. s
The CO2gas supply being connected should be industrial grade 99.5%
pure and should not contain siphon tubes. Install a two-stage pressure
regulator at the cylinder outlet. The high pressure gauge at the tank should
have 0-2000 psig range and the low pressure gauge, at the incubator inlet,
should have a 0-30 psig range. Input pressure to the incubator must be
maintained at 15 psig (103.4 kPa), ±5 psig.
The incubator has a serrated fitting on the back of the cabinet to connect
the gas supply. Refer to Figure 1-13. The fitting is labeled CO2 Inlet.
Make sure that the connection is secured with a clamp. Check fitting for
leaks.
Water Jacketed CO2 Incubator 1-9Thermo Scientific
Section 1
Installation and Start-Up
Fill the Humidity Pan
(continued)
Connect the CO2Gas
Supply

Warning This incubator is designed to be operated with CO2gas only.
Connecting a flammable or toxic gas can result in a hazardous condition.
Gases other than CO2should not be connected to this equipment. CO2
gas cylinders have UN1013 labeled on the cylinder and are equipped with
a CGA 320 outlet valve. Check the gas cylinder for the proper
identification labels. The CO2gas supply being connected to the incubator
should be industrial grade, 99.5% pure. Do not use CO2gas cylinders
equipped with siphon tubes. A siphon tube is used to extract liquid CO2
from the cylinder, which can damage the pressure regulator. Consult with
your gas supplier to ensure that the CO2cylinder does not contain a
siphon tube. Gas cylinders should also be secured to a wall or other
stationary object to prevent them from tipping.
A two-stage CO2pressure regulator is required to be installed on the outlet
valve of the gas cylinder. Input pressure to the incubator must be
maintained at 15 psig (103.4 kPa), ±5 psig, for proper performance of the
CO2control system. (A single stage CO2pressure regulator will not
maintain 15 psig.
If higher purity CO2is desired inside the incubator (greater than 99.5%
pure), the pressure regulator should be constructed with a stainless steel
diaphragm along with specifying the purity of the CO2from the gas
supplier. Follow the manufacturer’s instructions to ensure proper and safe
installation of the pressure regulator on the gas cylinder. Consult your
facility safety officer to ensure that the equipment is installed in accordance
with the codes and regulations that are applicable in your area. s
Now that the incubator has been properly installed, connected to power,
filled with water, humidity pan filled, and connected to gas supply, system
setpoints can be entered. The temperature, over temperature, and CO2.
setpoints can now be entered.
The temperature setpoint range is 10° to 55°C, settable to within 0.1°C.
The lowest temperature the incubator can control is +5°C above the
ambient temperature at the incubator’s location. This incubator is shipped
from the factory with a temperature setpoint of 10°C. At this setting, all
heaters and alarms are turned off.
1-10 Water Jacketed CO2 Incubator Thermo Scientific
Section 1
Installation and Start-Up
Connect the CO2 Gas
Supply (continued)
Incubator Start-Up
Set the Operating
Temperature Setpoint

To change the temperature setpoint:
1. Press the Mode key until the Set Temp indicator lights (see illustration
above).
2. Press the up/down arrow until the desired temperature setpoint is
displayed.
3. Press Enter to save the setpoint.
4. Press the Mode key until the Run Indicator lights to go to Run mode.
Caution The overtemp circuit is designed as a safety to protect the
incubator only. It is not intended to protect or limit the maximum
temperature of the cell cultures or customer’s equipment inside the
incubator if an overtemp condition occurs. s
The factory setting for the Overtemperature setpoint is 40°C. It can be set
over a range of temp setpoint +0.5°C to 60°C. If the temperature setpoint
is moved above the Overtemp setpoint, the Overtemp will automatically
update to 1.0°C + the temp setpoint. It is recommended that the
Overtemp setpoint be 1°C over the temp setpoint.
To set the Overtemp setpoint:
1. Press the Mode key until the Set Otemp indicator lights (see
illustration above).
2. Press the up/down arrow until the desired Overtemp setpoint is
displayed.
3. Press Enter to save the setpoint.
4. Press the Mode key until the Run Indicator lights to go to Run mode.
Water Jacketed CO2 Incubator 1-11Thermo Scientific
Section 1
Installation and Start-Up
Set the Overtemp Setpoint
Set the Operating Temp
Setpoint (continued)