Thermo Scientific 310 Series User manual

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User Manual
Forma Direct Heat CO2 Incubator
Model 310 Series
Operatign and Maintenance Manual 7010310 Rev. 16

Thermo ScientificThermo Scientific
Preface
Model 310 Series DH Incubator i
Preface
Model CO2 Sensor* Voltage**
310 T/C 115
311 T/C 230
320 IR 115
321 IR 230
350† T/C 115
351† T/C 230
360† IR 115
361† IR 230
* T/C is a thermal conductivity sensor.
IR is an infrared sensor.
** All units are 50/60 Hz.
† Copper interior
MANUAL NUMBER 7010310
16 31343/IN-4592 10/22/14 New magnetic door switch (exploded drawing, electrical schematics) RoHS ccs
15 29174/IN-4469 3/21/14 Solenoid change - update 203 drawing and schematics (pgs 8-5 & 9-1 to 9-3) ccs
14 30649/IN-4446 1/28/14 Updated electrical schematics - display board ccs
13 29704/IN-4310 3/29/13 Changed wingnut in blower assembly to hex nut (310-202-1-D) ccs
12 28116/IN-4201 5/29/12 Chg’d harness from 190522 to 1900089 - pg 8-6 ccs
11 27990/IN-4179 5/3/12 Added Intended Use to pg ii, updated Specs uniformity to 0.3°C from 0.2 ccs
10 27292/IN-4073 9/28/11 Switcher from 400199 to 400201 kit - pg 8-1 ccs
-- -- 7/13/10 Re-installed “Specs are based on...” on Specification page. Unintentional removal ccs

Thermo Scientificii Model 310 Series DH Incubator
Preface
Contains Parts and Assemblies
Susceptible to Damage by
Electrostatic Discharge (ESD)
CAUTION
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s
Warning If this unit is not used in the manner specified in this operating manual, the protection provided by
the equipment design may be impaired. s
Intended Use: The 310 Series Direct Heat Incubators are designed to maintain an optimal environment for
the incubation of tissue and cell samples. These models are designed to maintain temperature and Carbon
Dioxide levels as set by the operator as well as monitoring chamber relative humidity. CO2 is controlled using
either a thermo conductivity cell, or an infra-red sensor. T
The incubators are approved for general purpose use as a Microbiological Incubator (21 CFR 866.2540) and
are also approved use in assisted reproductive procedures for the incubation of ova and embryos (21 CFR
884.6120).
Non-intended Use: The 310 Series Direct Heat Incubators are not intended for use where electrical or physical
contact with the patient is established.
These incubators are not intended to be operated in potentially explosive environments and are not intended
for use with flammable materials.
Caution All internal adjustments and maintenance must be performed by qualified service personnel. s
Material in this manual is for information purposes only. The contents and the product it describes are subject
to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related
to the use of this manual.
©2003 Thermo Fisher Scientific. All rights reserved.

Thermo Scientific Model 310 Series DH Incubator iii
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.
Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market
after 13 August 2005.
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Fisher
Scientific has contracted with one or more recycling/disposal companies in each EU Member State
European Country, and this product should be disposed of or recycled through them. Further
information on Thermo’s compliance with this directive, the recyclers in your country and
information on Thermo Scientific products will be available at www.thermo.com.
4Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4Always dissipate extreme cold or heat and wear protective clothing.
4Always follow good hygiene practices.
4Each individual is responsible for his or her own safety.

Thermo Scientificiv Model 310 Series DH Incubator
Preface
Do You Need Information or Assistance on
Thermo Scientific Products?
If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:
1-740-373-4763 Direct
1-800-438-4851 Toll Free, U.S. and Canada
1-877-213-8051 FAX
http://www.thermoscientific.com Internet Worldwide Web Home Page
[e-mail] Tech Support Email Address
Certified Service Web Page
Thermo Fisher Scientific
401 Millcreek Road, Box 649
Marietta, OH 45750
Our staff can provide information on pricing and give you quotations. We can
take your order and provide delivery information on major equipment items or make
arrangements to have your local sales representative contact you. Our products are listed on the
Internet and we can be contacted through our Internet home page.
Our staff can supply technical information about proper setup, operation or
troubleshooting of your equipment. We can fill your needs for spare or replacement parts or
provide you with on-site service. We can also provide you with a quotation on our Extended
Warranty for your Thermo Scientific products.
Whatever Thermo Scientific products you need or use, we will be happy to discuss your
applications. If you are experiencing technical problems, working together, we will help you
locate the problem and, chances are, correct it yourself...over the telephone without a service
call.
When more extensive service is necessary, we will assist you with direct factory trained
technicians or a qualified service organization for on-the-spot repair. If your service need is
covered by the warranty, we will arrange for the unit to be repaired at our expense and to your
satisfaction.
Regardless of your needs, our professional telephone technicians are available to assist you
Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by
telephone or fax. If you wish to write, our mailing address is:
International customers, please contact your local Thermo Scientific distributor.
Sales Support
Service Support
www.unitylabservices.com

Thermo Scientific Model 310 Series DH Incubator vThermo Scientific
Preface
Warranty Notes
Information You Should Know Before Requesting Warranty Service
•Locate themodel and serial numbers.Aserial tag is located on the unit itself.
•For equipment service or maintenance, or with technical or special application inquiries, contact Technical
Services at 1-800-438-4851 or 1-740-373-4763 (USA and Canada). Outside the USA, contact your local
distributor.
Repairs NOT Covered Under Warranty
•Calibration of control parameters.Nominal calibrations are performed at the factory; typically ±1°C for
temperature, ±1% for gases, and ±5% for humidity. Our service personnel can provide precise calibrations as
abillable service at your location. Calibration after awarranty repair is covered under the warranty.
•Damage resulting from use of improper quality water, chemicals or cleaning agents detrimental to
equipment materials.
•Service calls for improper installation or operating instructions. Corrections to any of the following
are billable services:
1) electrical service connection
2) tubing connections
3) gas regulators
4) gas tanks
5) unit leveling
6) room ventilation
7) adverse ambient temperature fluctuations
8) any repair external to the unit
•Damage resulting from accident, alteration, misuse, abuse, fire, flood, acts of God, or improper
installation.
•Repairs to parts or systems resulting from unauthorized unit modifications.
•Any labor costs other than that specified during theparts and labor warranty period, which may
include additional warranty on CO2sensors, blower motors, water jackets, etc.

Model 310 Series DH Incubator viThermo Scientific
Table ofContents
Installation and Start-Up ......................................1-1
Control Panel Keys, Displays & Indicators . . . . . . . . . . . . . . . . . . . .1-2
Keypad Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Displays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Installing the Incubator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Incubator Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-11
Calibration ..................................................2-1
Calibrating the Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Calibrating the T/C CO2System . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2
Calibrating the Infrared CO2System . . . . . . . . . . . . . . . . . . . . . . . . .2-3
Calibrating Relative Humidity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-4
Configuration ................................................3-1
Turn the Audible Alarm ON/OFF . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Set an Access Code . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Set a Low Temp Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
Enable the Low Temp Alarm to Trip Contacts . . . . . . . . . . . . . . . . .3-2
Set a Low CO2Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3
Setting a High CO2Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3
Enable CO2Alarms to Trip Contacts . . . . . . . . . . . . . . . . . . . . . . . .3-4
Set New Zero Number for T/C CO2Sensors . . . . . . . . . . . . . . . . . .3-4
Set New Span Number for T/C CO2Sensors . . . . . . . . . . . . . . . . . .3-5
Set a Low RH Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-5
Enable RH Alarms to Trip Contacts . . . . . . . . . . . . . . . . . . . . . . . . .3-6
Enable Temp/RH to be Displayed . . . . . . . . . . . . . . . . . . . . . . . . . . .3-6
Select Primary Tank w/ Gas Guard Option . . . . . . . . . . . . . . . . . . . .3-7
Disable the Gas Guard System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-7
Communications Address for RS485 . . . . . . . . . . . . . . . . . . . . . . . .3-8
Alarms ......................................................4-1
Temp Control Failure Alarm TMP CNTR ERR . . . . . . . . . . . . . . .4-2
Sensor Fault Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
CO2 SNSR ERR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
IR AUTOZ ERR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Section 1
Section 2
Section 3
Section 4

vii Model 310 Series DH Incubator Thermo Scientific
Routine Maintenance .........................................5-1
Disinfecting the Incubator Interior . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Cleaning the Cabinet Exterior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2
Cleaning the Glass Doors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2
Cleaning the Humidity Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
Reversing the Door Swing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
HEPA Filter (Factory Installed Option) . . . . . . . . . . . . . . . . . . . . . . .5-8
Replacing the Power Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-8
Major Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-9
Electronics Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-9
Replacing the Sample Air Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-10
FactoryOptions ..............................................6-1
Connect Remote Alarm Contacts . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Connect RS485 Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
Connecting the Analog Output Boards . . . . . . . . . . . . . . . . . . . . . . .6-2
CO2Gas Guard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-5
Humidity Readout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-6
Uninterruptible Power Supply Connections . . . . . . . . . . . . . . . . . . .6-7
Specifications ...............................................7-1
Spare Parts ..................................................8-1
Electrical Schematics ........................................9-1
WarrantyInformation ........................................10-1
Table of Contents
Section 5
Section 6
Section 8
Section 7
Section 9
Section 10

Model 310 Series DH Incubator 1-1Thermo Scientific
Section 1 Installation and Start-Up
Incubator components are described below.
Figure 1-1.Model 310 Series Direct Heat Incubator
• Outer Door - Reversible to opposite swing, see Section 5
• Inner Door - Reversible to opposite swing, see Section 5
• Chamber Gas Sample Port - Used for sampling chamber CO2content
using a FYRITE or similar instrument.
• Main Power Switch
• Control Panel - Keypad, Displays and indicators (See Figure 1-2)
• Leveling Legs - Used to level the unit
Note The incubators are stackable. Information follows. s

1-2 Model 310 Series DH Incubator Thermo Scientific
Section 1
Installation and Start-up
Silence - Silences the audible alarm.
Alarm Indicator - Light pulses on/off during an alarm condition in the
cabinet.
Mode Select Switch - Used to select Run, Setpoints, Calibration and
System Configuration Modes.
Message Center - Displays system status.
Mode Select Indicators -
Run: Run Menu
Set: Set Points Menu
Cal: Calibrate Menu
Config: Configuration Menu
Up and Down Arrows - Increases or decreases the number values, toggles
between choices.
Enter - Stores the value into computer memory.
Heat Indicator - Lights when power is applied to the heaters.
Temp Display - Program to display temperature continuously, RH con-
tinuously (with RH option), or toggle between temperature and humid-
ity (with RH option). See Section 3, Configuration.
Scroll for Parameters Arrows - Moves the operator through the choices
of the selected mode.
CO2Inject Indicator - Lights when CO2is being injected into the incu-
bator.
% CO2 Display - Displays CO2percentage continuously.
Control Panel Keys,
Displays & Indicators
Figure 1-2.Control Panel

Model 310 Series DH Incubator 1-3Thermo Scientific
Section 1
Installation and Start-up
The Model 310 Series direct heat incubator has four basic modes which
allow incubator setup: Run, Setpoints, Calibration and System
Configuration.
Run is the default mode which the incubator will normally be in during
operation.
Set is used to enter system setpoints for incubator operation.
Calibration is used to calibrate various system parameters.
Configuration allows for custom setup of various options.
The chart below shows the selections under each of the modes.
1T/C units only Base Unit Displays
2IR units only Option Displays
*T/C units only
Scroll for Parameters Arrows: Steps the operator through the parameters
of SET, CAL and CONFIG Modes. The right arrow goes to the next
parameter, the left arrow returns to the previous parameter.
Up Arrow: Increases or toggles the parameter value that has been selected
in the SET, CAL, and CONFIG Modes.
Enter: Must press Enter key to save to memory all changed values.
Down Arrow: Decreases or toggles the parameter values that have been
selected in the SET, CAL and CONFIG Modes.
Silence Key: Press to silence the audible alarm. See Section 4 for alarm
ringback times.
Keypad Operation
RUN SETPOINTCALIBRATION CONFIGURATION
Default Mode Temperature Temp Offset Audible
Overtemp CO2Cal1Access Code
CO2IR Cal2Temp Lo Alarm
RH Cal Temp Relay
CO2 Lo Alarm
CO2 Hi Alarm
CO2 Relay
CO2 Z & S #'s *
RH Lo Alarm
RH Relay
Display Temp
Display Rh
Tank Select
Gas Guard
RS485 Address

Message Center: Displays the system status (Mode) at all times. Displays
SYSTEM OK during normal operation, or alarm messages if the system
detects an alarm condition. See Section 4, Alarms.
Upper and Lower Displays: These 7 segment displays vary depending
upon the options present and the configuration chosen. The upper dis-
play can display temp or RH, or toggle between them. The bottom dis-
play shows CO2continuously.
Warning Single and stacked units must be installed against a wall or
similar structure. Maintain a three-inch clearance behind the incubator for
electrical and gas hook-ups. s
1. Locate the unit on a firm level surface capable of supporting the unit’s
weight of 205 lbs.
2. Locate the unit away from doors and windows and heating and air
conditioning ducts.
3. Lift the unit only by the sides of the cabinet base . Do not attempt to
lift it by the front and back . This places stress on the outer door
hinges.
Caution When stacking incubators, the direct heat incubator must be the
top unit. Never stack a water-jacketed incubator on top of a Model 310
Series unit. s
Warning With incubators in a stacked configuration, do not leave both
exterior doors open at the same time. s
Warning If the units have been in operation, shut them both off and
disconnect from the power source before beginning any service work. s
Note Two stacking brackets (shown at right) are included
in the parts bag shipped with each incubator. s
1-4 Model 310 Series DH Incubator Thermo Scientific
Section 1
Installation and Start-Up
Displays
Installing the
Incubator
Stacking the Incubators
Figure 1-3.
Stacking Brackets

1. Remove and discard the slotted head
screws on the top of the bottom incubator
(Figure 1-4). Replace with 5/16 HH bolts
and flatwashers included in the stacking
kit. Do not tighten.
2. Remove the kickplate from below the door on the top incubator
(Figure 1-5). Two Phillips screws secure the plate. Note the notches at
the base of the incubator frame.
3. Disconnect the door cable from the connector behind the plate. Lift
the door off the hinge pins and set it aside. The hinge pins are not
attached to the brackets and may fall out when the door is lifted off.
4. Unscrew and remove the leveling feet from the top unit and lift it onto
the bottom unit, offsetting the base of the top unit approximately 2-3
inches behind the screws identified by the arrows in Figure 1-4.
Warning This incubator weighs 205 lbs. Have sufficient personnel
available when lifting. Lift the unit only by the sides of the cabinet base.
Do not attempt to lift it by the front and back as this places stress on the
outer door hinge. s
5. Align the sides of the top unit with the bottom unit and slide the top
unit forward until the notches in the base of the top unit align with
the bolts in the top of the bottom unit. The flatwashers should slide
over the base frame. Tighten bolts.
6. Remove the four nylon plugs on the lower back of the upper incubator.
7. Insert the stacking brackets into the slots on the back of the control
panel of the bottom unit as shown in Figure 1-6.
Model 310 Series DH Incubator 1-5Thermo Scientific
Section 1
Installation and Start-Up
Stacking (continued)
Figure 1-4.Slotted Head Screws Figure 1-5.Align
Figure 1-6.Brackets Installed

8. Align the slots in the brackets with the mounting holes on the back of
the top incubator. Secure the brackets with the screws provided in the
stacking kit.
9. Make sure the hinge pins are in the hinge brackets. Slide the door onto
the hinge pins.
10. Secure the door heater cable to the connector below the door and
replace the kick plate on the top unit, taking care not to crimp cable.
11. The stacked incubators are ready for service.
Remove vinyl from shelf channels, duct sheets, and air duct, if present.
Using a suitable laboratory disinfectant, disinfect all interior surfaces
including shelves and shelf supports, door gaskets, blower wheel and CO2
sensor. Refer to Section 5.
Caution Before using any cleaning or decontamination method except
those recommended by the manufacturer, users should check with the
manufacturer that the proposed method will not damage the equipment.
Accidental spills of hazardous materials on or inside this unit are the
responsibility of the user. s
1. Install the large sheet metal shelf supports with the tabs facing into the
center of the chamber with their slots up. There are no right side or
left side supports. Simply turn one of them to fit the opposite side. Tilt
the shelf supports as they are placed in the chamber so the tops fit into
the top air duct, then guide them into the vertical position. Figure 1-7
shows the support as it would be oriented for right side of chamber.
1-6 Model 310 Series DH Incubator Thermo Scientific
Section 1
Installation and Start-Up
PreliminaryCleaning and
Disinfecting
Installing the Shelves
Figure 1-7.Shelf Channels and Ductwork
Stacking (continued)

2. Note there is no difference in the left side and right side shelf channels.
3. Install the shelf channels by placing the channel’s rear slot over the
appropriate rear tab on the shelf support. Pull the shelf channel
forward and engage the channel’s front slot into the shelf support’s
appropriate forward tab. Refer to Figure 1-8.
Locate the opening in the top left corner on the inside of the chamber.
Remove the tape covering the opening on the outside of the unit. Locate
the stopper with filter in the hardware bag. Install the assembly in the
opening inside the chamber. See Figure 1-9.
Figure 1-9.Filter and Sensor Locations
Caution Be careful when handling the filter. The media can be damaged if
it is mishandled.
To avoid damage to the incubator, do not operate the unit without the
HEPA filter in place. s
1. Remove the filter from the shipping box.
2. Remove the plastic coating from the filter, using caution not to touch
the filter media.
3. Install the filter as shown in Figure 1-9. Refer to Section 5 for HEPA
filter maintenance.
Model 310 Series DH Incubator 1-7Thermo Scientific
Section 1
Installation and Start-Up
Figure 1-8.Channel Ends
HEPA Filter
Sample Air Filter
Temperature
sensor
Access
port filter
Installing (optional) HEPA
Filter
Installing Access Port
Filter
Installing the Shelves
(continued)

Leveling the Unit
1. Remove the filter from the shipping bag.
2. Install the air sample filter assembly to the black hose barb behind the
top duct.
3. Insert the other end of the filter assembly into the larger hole in the air
intake to the blower. Refer to Figure 1-10.
4. Verify that the temperature sensor is installed into the smaller hole in
the intake, as in Figure 1-10.
Check for level by placing a bubble style level on one of the shelves. Turn
the hex nut on the leveler counterclockwise to lengthen the leg or
clockwise to shorten it. Level the unit front-to-back and left-to-right.
See the serial tag on the side of the unit for electrical specifications or refer
to the electrical schematics at the end of this manual.
Caution Connect the incubator to a grounded, dedicated circuit.
The power cord connector is the mains disconnect device for the
incubator. Position the incubator so the unit can be easily disconnected. s
1-8 Model 310 Series DH Incubator Thermo Scientific
Section 1
Installation and Start-Up
Figure 1-10.View of Chamber Ceiling
Installing the Air Sample
Filter
Connecting the Unit to
Electrical Power

Plug the provided power cord into the power inlet connector on the back
of the cabinet (See Figure 1-11), then into the grounded, dedicated,
electrical circuit.
Figure 1-11.Connections on Unit Back
Fill the humidity pan with sterile pure water to within 1/2” of the top.
Place the pan directly in the center of the incubator floor to ensure best
humidity and temperature response.
For applications requiring abnormally high humidity, the pan should be
placed against the left side wall of the incubator. The ductwork has been
modified to allow the pan to be placed against the wall of the incubator.
In some ambient conditions, this may cause condensation to form in the
chamber.
For best operation of the incubator, sterilized distilled, demineralized or
de-ionized water should be used in the humidity pan. Water purity should
be in the resistance range of 50K to 1M Ohm/cm, or a conductivity range
of 20.0 to 1.0 uS/cm. Refer to ASTM Standard D5391-93 or D4195-88
for measuring water purity.
Distillation systems, as well as some types of reverse osmosis water purity
systems, can produce water in the quality range specified. Tap water is not
recommended as it may contain chlorine, which can deteriorate the
stainless steel. Tap water may also have a high mineral content, which
would produce a build-up of scale in the pan. High purity or ultra pure
water is not recommended as it is an extremely aggressive solvent and will
deteriorate the stainless steel. High purity water has a resistance of above
1M to 18M Ohm. Even high purity water can contain bacteria and
organic contaminants. Water should always be sterilized or treated with a
decontaminant, safe for use with stainless steel as well as safe for the
product, prior to being introduced into the humidity pan.
Model 310 Series DH Incubator 1-9Thermo Scientific
Section 1
Installation and Start-Up
Filling the HumidityPan
Connecting the Unit to
Electrical Power (cont.)

Caution Distilled or de-ionized water used in the humidity pan must be
within a water quality resistance range of 50K to 1M Ohm/cm to protect
and prolong the life of the stainless steel. Use of chlorinated water, or
decontamination products containing chlorine, will deteriorate the stainless
steel and cause rust, voiding the warranty. s
Check the level and change the water frequently to avoid contamination.
Do not allow the water level to fluctuate significantly. “Dry-outs” will have
an adverse effect on the humidity level and CO2calibration of the T/C
units.
Warning High concentrations of CO2gas can cause asphyxiation! OSHA
Standards specify that employee exposure to carbon dioxide in any eight-
hour shift of a 40-hour work week shall not exceed the eight-hour time
weighted average of 5000 PPM (0.5% CO2). The short term exposure
limit for 15 minutes or less is 30,000 ppm (3% CO2). Carbon dioxide
monitors are recommended for confined areas where concentrations of
carbon dioxide gas can accumulate. s
Warning This incubator is designed to be operated with CO2gas only.
Connecting a flammable or toxic gas can result in a hazardous condition.
Gases other than CO2should not be connected to this equipment. CO2
gas cylinders have a UN1013 label on the cylinder and are equipped with a
CGA 320 outlet valve. Check the gas cylinder for the proper identification
labels. The CO2gas supply being connected to the incubator should be
industrial grade, 99.5% pure. Do not use CO2gas cylinders equipped with
siphon tubes. A siphon tube is used to extract liquid CO2from the
cylinder which can damage the pressure regulator. Consult with your gas
supplier to ensure that the CO2cylinder does not contain a siphon tube.
Gas cylinders should also be secured to a wall or other stationary object to
prevent them from tipping.
A two-stage CO2pressure regulator is required to be installed on the outlet
valve of the gas cylinder. Input pressure to the incubator must be
maintained at 15 psig (103.4 kPa), ±5 psig, for proper performance of the
CO2control system. A single stage CO2pressure regulator will not
maintain 15 psig (103.4 kPa) to the incubator as the pressure in the CO2
cylinder decreases; therefore, a two stage regulator is recommended.
If higher purity CO2is desired inside the incubator (greater than 99.5%
pure), the pressure regulator should be constructed with a stainless steel
diaphragm, along with specifying the purity of the CO2from the gas
supplier. Follow the manufacturer’s instructions to ensure proper and safe
installation of the pressure regulator on the gas cylinder.
Consult your facility safety officer to ensure that the equipment is installed
in accordance with the codes and regulations applicable in your area. s
1-10 Model 310 Series DH Incubator Thermo Scientific
Section 1
Installation and Start-Up
Connecting the CO2Gas
Supply
Filling the HumidityPan
(cont.)

The CO2gas supply being connected should be industrial grade 99.5%
pure and should not contain siphon tubes. Install a two-stage pressure
regulator at the cylinder outlet. The high pressure gauge at the tank should
have 0-2000 psig range. The low pressure gauge, at the incubator inlet,
should have a 0-30 psig range. Input pressure to the incubator must be
maintained at 15 psig (103.4 kPa).
The incubator has serrated fittings on the back of the cabinet to connect
the gas supply. Refer to Figure 1-11. The fitting is labeled CO2 Inlet #1
Tank. Make sure that the connections are secured with clamps. Check all
fittings for leaks.
For units having the CO2Gas Guard option, refer to Section 6.
With the incubator properly installed and connected to power, the
humidity pan filled, and the unit connected to gas supplies, system
setpoints can be entered. The following setpoints can be entered in Set
mode: Temperature, Overtemperature and CO2. To enter Set mode, press
the Mode key until the Set indicator lights. Press the right and/or left
arrow keys until the proper parameter appears in the message center. See
Chart 1-1 for more detail.
Model 310 Series incubators have an operating temperature range of 10°C
to 50°C, depending on ambient temperature. The incubator is shipped
from the factory with a temperature setpoint of 10°C. At this setting, all
heaters are turned off. To change the operating temperature setpoint:
1. Press the Mode key until the Set indicator lights.
2. Press the right arrow until “Temp XX.X” is displayed in the message
center.
3. Press the up/down arrow key until the desired temperature setpoint is
displayed.
4. Press Enter to save the setpoint.
5. Press the Mode key until the Run indicator lights for Run mode or
press the right/left arrow keys to go to next/previous parameter.
Model 310 Series DH Incubator 1-11Thermo Scientific
Section 1
Installation and Start-Up
Incubator Start-Up
Setting the Operating
Temperature
Connecting the CO2gas
Supply(continued)