Thermo Scientific invitrogen EVOS OSI-2 User manual

For Research Use Only. Not for use in diagnostic procedures.
EVOS™Onstage Incubator (OSI-2)
USER GUIDE
for use with:
EVOS™M5000 Imaging System
EVOS™M7000 Imaging System
Catalog NumberAMC2000
Publication Number MAN0027987
Revision A.0

Life Technologies Corporation | 22025 20th Ave SE Ste. 100 | Bothell, Washington 98021 USA
Revision history:MAN0027987 A.0 (English)
Revision Date Description
A.0 6 March 2023 Initial release.
The information in this guide is subject to change without notice.
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LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR
ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these
products, you accept the terms and conditions of all applicable Limited Use Label Licenses.
TRADEMARKS: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
©2023 Thermo Fisher Scientific Inc. All rights reserved.

Contents
■CHAPTER1Productinformation .................................................. 5
Productdescription ............................................................. 5
EVOS™Onstage Incubator(OSI-2) ............................................ 5
Standard itemsincluded ..................................................... 5
Required materials not provided .............................................. 6
Incubatorcomponents ........................................................... 7
Control unit and Environmental chamber ...................................... 7
Control unit (backview) ...................................................... 8
Status LEDmodes .......................................................... 9
Enviromentalchamber ..................................................... 10
■CHAPTER2Methods ............................................................. 12
Set up the EVOS™Onstage Incubator(OSI-2) ...................................... 12
Install the EVOS™Onstage Incubator (OSI-2)  ................................. 12
Set up the EVOS™Onstage Incubator (OSI-2) for operation ..................... 15
Use the Onstage Incubator with the EVOS™M5000 Imaging System ................. 19
Power on the EVOS™Onstage Incubator(OSI-2) ............................... 19
Optional: Set Ambient Temperature Oset .................................... 22
Configure gasinputs ....................................................... 23
Calibrate incubator oxygensensor ........................................... 24
Use the Onstage Incubator with the EVOS™M7000 Imaging System ................. 27
Power on the EVOS™Onstage Incubator(OSI-2) ............................... 27
Configure gasinputs ....................................................... 29
Calibrate oxygensensor .................................................... 31
■CHAPTER3Troubleshooting ..................................................... 33
Troubleshooting ................................................................ 33
■APPENDIXASpecifications ...................................................... 35
Physical characteristics ......................................................... 35
Hardware ..................................................................... 35
EVOS™Onstage Incubator (OSI-2) User Guide 3

■APPENDIXBCompatible vessel holders and stage plates ................... 36
EVOS™Onstage Incubator (OSI-2)-compatible vessel holders and stage plates ....... 36
■APPENDIXCSafety ............................................................... 39
Symbols on thisinstrument ..................................................... 39
Standard safetysymbols ................................................... 39
Location of safetylabels .................................................... 40
Control and connectionsymbols ............................................. 41
Conformitysymbols ........................................................ 41
Safety information for instruments not manufactured by Thermo FisherScientific ...... 42
Instrumentsafety .............................................................. 42
General ................................................................... 42
Electricalsafety ........................................................... 42
Safety requirements for EVOS™Onstage Incubator(OSI-2) ...................... 43
Cleaning anddecontamination .............................................. 44
Instrument component and accessorydisposal ................................ 44
Safety and electromagnetic compatibility (EMC) standards .......................... 45
Safety standards .......................................................... 45
EMC standards ............................................................ 45
Environmental design standards ............................................. 46
Chemicalsafety ................................................................ 46
Chemical wastesafety .......................................................... 47
Chemical waste hazard ..................................................... 47
Chemical waste safetyguidelines ............................................ 47
Wastedisposal ............................................................ 47
Biological hazardsafety ......................................................... 48
■APPENDIXDDocumentation and support ...................................... 49
Customer and technical support ................................................. 49
Limited product warranty ........................................................ 49
Contents
4EVOS™Onstage Incubator (OSI-2) User Guide

Product information
IMPORTANT! Before using this product, read and understand the information in the “Safety” appendix
in this document.
Product description
EVOS™Onstage Incubator (OSI-2)
The EVOS™Onstage Incubator (OSI-2) is an optional accessory for the EVOS™M5000 and EVOS™
M7000 Imaging Systems that enables the incubation of cells at user-defined temperature, humidity, and
gas concentration (O2, CO2), allowing the capture of images from the same sample over long periods of
time and recording of time lapse movies.
The EVOS™Onstage Incubator (OSI-2) consists of a stagetop environmental chamber that is placed on
the X‑Y stage of the imaging system and a separate control unit that supplies the power and humidified
gas (air or air-CO2premix, CO2-only, and N2-only). The second generation EVOS™Onstage Incubator
contains an on‑board air compressor that enables the use of on‑board air instead of a separate air tank.
The Onstage Incubator (OSI-2) is controlled by the same software and user interface that controls the
imaging system.
Standard items included
•Stagetop Environmental Chamber
•Control Unit
•Master Stage Plate (also available separately as EVOS™Onstage Master Plate,
Cat.No.AMEPVH106)
•Vessel holder for multiwell plates (also available separately as EVOS™Onstage Vessel Holder,
Multiwell Plates, Cat.No.AMEPVH028)
•Light shield and light shield cover
•Sensor data cable with 6-pin connector
•Cable, USB2.0A‑to‑B, 180cm (6ft)
•Heated hose with temperature control, 180 cm (6 ft) (also available separately as EVOS™Onstage
Incubator Hose, Cat.No.AMEP4728)
•Gas line, 1/8 in. ID, ¼ in. OD (also available separately as EVOS™Onstage Incubator Gas Line,
Cat.No.AMEP4732, for an extra 25ft)
•Push-to-connect gas line adaptor, ¼in. NPT male (3each)
•Standard-head open-end wrench
•Hex screw driver (2mm)
1
EVOS™Onstage Incubator (OSI-2) User Guide 5

•Power Cord, Type A (North America)
Note: A country-specific power cord must be ordered separately in regions not using the TypeA
power plug.
Required materials not provided
•CO2gas or CO2/air premix for CO2regulation
•N2gas for O2regulation (if performing hypoxia experiments)
•Multistage regulators capable of providing continuous 40–50 psi output pressure for all gas sources
used with the OSI-2. The regulators must have a ¼ in. NPT female output; if the regulator has a
¼ in. NPT male output, a male-to-female adaptor is needed.
Chapter1Product information
Product description
1
6EVOS™Onstage Incubator (OSI-2) User Guide

Incubator components
Control unit and Environmental chamber
2
4
7
1
6
3
5
Figure1EVOS™Onstage Incubator (OSI-2) Control unit and Environmental chamber
1Control unit
2Heated hose
3Hose heater connector
4Water reservoir door
5Water reservoir door release
6Environmental chamber
7Sensor data cable with 6‑pin connector
Chapter1Product information
Incubator components 1
EVOS™Onstage Incubator (OSI-2) User Guide 7

Control unit (back view)
Figure2Back view of the OSI-2 Control unit showing the locations of the gas ports, input jacks, and the
power switch
1Port 1: Air or air‑CO2premix
2Port 2: CO2
3Port 3: N2
4Power switch
5Power input jack
66‑pin sensor data cable jack
7USB control cable jack
Chapter1Product information
Incubator components
1
8EVOS™Onstage Incubator (OSI-2) User Guide

Status LED modes
The status indicator LED on the OSI-2 control unit identifies the status of the incubator.
Common Callouts and Arrows
1. Copy-paste a callout or arrow to use in this SVG.
Note: If you need more advanced callouts or arrows
use the TechComm_Inkscape_Callout&Arrow_Libary.
3. Delete this text, this rectangle, and unused
callouts, arrows, or other SVG elements
before adding this SVG to the repository.
2. Edit number and/or line-length, as needed.
1 1
1
1
1
Figure3OSI-2 status indicator LED on the control unit
1Status indicator LED
LED color LED mode Incubator status
Blue Solid The OSI-2 is powered on and connected to the computer, but no
functions have been enabled.
Blue Blinking The temperature/humidity/gas concentration features have been
enabled and the system is warming up.
Blue Slow blink–
fadein/fadeout
All temperature/humidity/gas concentration targets are met and being
maintained.
Amber Blinking The OSI-2 has deviated from the temperature/humidity/gas
concentration target.
Red Blinking The USB cable is connected, but the power switch is o or no power
supply is connected.
Chapter1Product information
Incubator components 1
EVOS™Onstage Incubator (OSI-2) User Guide 9

Enviromental chamber
The environmental chamber of the EVOS™Onstage Incubator (OSI-2) consists of the incubator
chamber, the vessel holder/adaptor, the heated glass lid, the light shield, and the light shield cover.
The environmental chamber sits on the onstage incubator master stage plate that is attached to the X-Y
stage of the imaging system.
6
5
3
2
1
4
7
Figure4Components of the Environmental chamber
1Light shield cover
2Light shield
3Heated glass lid
4Vessel holder/adaptor
5Incubator chamber
6Master stage plate
7Perfusion ports
Chapter1Product information
Incubator components
1
10 EVOS™Onstage Incubator (OSI-2) User Guide