Thermo Scientific KingFisher Plant DNA Kit User manual

Thermo Scientific KingFisher
Plant DNA Kit
Instruction Manual
Rev. 1.1

Thermo Scientific KingFisher
Plant DNA Kit
Instruction Manual
Rev. 1.1, Cat. no. N11996

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Thermo Fisher Scientific 5Thermo Scientific KingFisher Plant DNA Kit Instruction Manual
Contents
Chapter 1 Kit Content ...........................................................................................7
Chapter 2 Product Description.........................................................................11
Introduction ............................................................................11
Intended use .........................................................................11
Principle and procedure ........................................................11
Kit specications ......................................................................12
KingFisher magnetic particle processors ...................................13
Chapter 3 Safety Information............................................................................17
Chapter 4 Storage Conditions and Preparation of
the Working Solution ......................................................................19
Storage conditions ...................................................................19
Preparation of the RNaseA working solution ..........................19
Chapter 5 Protocols and Pipetting Instructions ...........................................21
Handling of KingFisher Magnetic Beads ..................................21
Sample material and storage .....................................................21
Homogenization of sample material .........................................22
Instructions for KingFisher Flex with 96 deep well plates
for DNA purication from 400µl of plant lysate .....................23
Summary of platecontents ...................................................25
Instructions for KingFisher Duo with 12-pin magnet head
and 96 deep well plates for DNA purication from
400µl of plant lysate ...............................................................26
Summary of plate and elution strip contents .........................28
Quantication and determination of the purity of DNA .........29
Chapter 6 General Information.........................................................................31
Reagent specicity and volumes ...............................................31
Handling of magnetic beads ....................................................31
Binding and wash steps ............................................................31
Elution step .............................................................................32