Thermo Scientific KingFisher Plant DNA Kit User manual

Thermo Scientific KingFisher
Plant DNA Kit
Instruction Manual
Rev. 1.1

Thermo Scientific KingFisher
Plant DNA Kit
Instruction Manual
Rev. 1.1, Cat. no. N11996

Copyright © 2011 ermo Fisher Scientic Inc. First edition published in 2010. All rights reserved.
Reproduction of the accompanying user documentation in whole or in part is prohibited.
“BindIt”, “KingFisher”, “Microtiter”, “Multidrop”, “NanoDrop” and "Versette" are registered
trademarks of ermo Fisher Scientic.
All other trademarks and registered trademarks are the property of their respective holders.
ermo Fisher Scientic reserves the right to change its products and services at any time to
incorporate technological developments. is manual is subject to change without prior notice as part
of a continuous product development. Although this manual has been prepared with every precaution
to ensure accuracy, ermo Fisher Scientic assumes no liability for any errors or omissions, nor
for any damages resulting from the application or use of this information. is instruction manual
supersedes all previous editions.
e Product will operate substantially in conformance with ermo Fisher Scientic’s published
specications.
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Thermo Fisher Scientific 5Thermo Scientific KingFisher Plant DNA Kit Instruction Manual
Contents
Chapter 1 Kit Content ...........................................................................................7
Chapter 2 Product Description.........................................................................11
Introduction ............................................................................11
Intended use .........................................................................11
Principle and procedure ........................................................11
Kit specications ......................................................................12
KingFisher magnetic particle processors ...................................13
Chapter 3 Safety Information............................................................................17
Chapter 4 Storage Conditions and Preparation of
the Working Solution ......................................................................19
Storage conditions ...................................................................19
Preparation of the RNaseA working solution ..........................19
Chapter 5 Protocols and Pipetting Instructions ...........................................21
Handling of KingFisher Magnetic Beads ..................................21
Sample material and storage .....................................................21
Homogenization of sample material .........................................22
Instructions for KingFisher Flex with 96 deep well plates
for DNA purication from 400µl of plant lysate .....................23
Summary of platecontents ...................................................25
Instructions for KingFisher Duo with 12-pin magnet head
and 96 deep well plates for DNA purication from
400µl of plant lysate ...............................................................26
Summary of plate and elution strip contents .........................28
Quantication and determination of the purity of DNA .........29
Chapter 6 General Information.........................................................................31
Reagent specicity and volumes ...............................................31
Handling of magnetic beads ....................................................31
Binding and wash steps ............................................................31
Elution step .............................................................................32

Thermo Fisher Scientific
Contents
6Thermo Scientific KingFisher Plant DNA Kit Instruction Manual
Appendix A Troubleshooting ................................................................................35
Appendix B Ordering Information .......................................................................37

Thermo Fisher Scientific 7Thermo Scientific KingFisher Plant DNA Kit Instruction Manual
Chapter 1
Kit Content
Table 1-1. Thermo Scientific KingFisher Plant DNA Kit
Item KingFisher Plant DNA Kit
Cat. No. 97050196
Package size 1 x 96 samples
KingFisher Magnetic Beads 3.2 ml
Lysis Buffer 55 ml
Binding Buffer 50 ml
Wash Buffer 1 65 ml
Wash Buffer 2 65 ml
Wash Buffer 3 65 ml
Elution Buffer 20 ml
RNase A (lyophilized) 1 vial
Recommended instrument KingFisher Flex with
96 deep well head
KingFisher Duo
KingFisher 96
e KingFisher Plant DNA Kit (Cat. No. 97050196)
is intended for the purication of 96 samples using the
ermo Scientic KingFisher Flex with a 96 deep well
head or the ermo Scientic KingFisher Duo and
a lysed sample volume of 400µl.

Thermo Fisher Scientific8Thermo Scientific KingFisher Plant DNA Kit Instruction Manual
Kit Content
Equipment and reagents to be supplied by the user:
• 80% ethanol (EtOH), molecular biology grade
• DNase-free water
• Homogenization equipment
• Magnetic particle processor
Table 1-2. Thermo Scientific KingFisher magnetic particle processors
Cat. No. Product
5400000 KingFisher magnetic particle processor
5400050 KingFisher mL magnetic particle processor
5400100 KingFisher Duo magnetic particle processor
5400630 KingFisher Flex magnetic particle processor with 96 deep well head
5400640 KingFisher Flex magnetic particle processor with 24 deep well head
Discontinued KingFisher 96 magnetic particle processor
Table 1-3. Thermo Scientific KingFisher Flex consumables
Cat. No. Product Package size
97002514 KingFisher Flex 96 tip comb for PCR magnet 80 pcs
97002524 KingFisher Flex 96 tip comb for KF magnet 100 pcs
97002534 KingFisher Flex 96 tip comb for deep well magnet 100 pcs
97002610 KingFisher Flex 24 deep well tip comb and plate 50 pcs
97002540 KingFisher Flex 96 KF plate (200 µl) 48 pcs
95040450 Microtiter deep well 96 plate, non sterile 50 pcs
95040460 Microtiter deep well 96 plate, sterile 50 pcs
95040470 KingFisher Flex 24 deep well plate 50 pcs
95040480 KingFisher Flex 24 deep well plate, sterile 50 pcs

Thermo Fisher Scientific 9Thermo Scientific KingFisher Plant DNA Kit Instruction Manual
Kit Content
Table 1-4. Thermo Scientific KingFisher Duo consumables
Cat. No. Product Package size
97003500 KingFisher Duo 12-tip comb for Microtiter deep well 96 plate 50 pcs
97003510 KingFisher Duo 6-tip combs and KingFisher 24 deep well plate
(12 pcs of 24 deep well plates, each including 4 tips combs)
48 pcs
97003520 KingFisher Duo elution strip 40 pcs
97003530 KingFisher Duo Combi pack for Microtiter deep well 96 plate
(tips combs, plates and elution strips for 96 samples)
1 box
Table 1-5. Thermo Scientific KingFisher mL consumables
Cat. No. Product Package size
97002111 KingFisher mL tip comb 800 pcs
97002121 KingFisher mL tube 20 x 45 pcs
97002131 KingFisher mL combi (tubes and tip combs for 60 samples) 60
97002141 KingFisher mL combi (tubes and tip combs for 240 samples) 240
Table 1-6. Thermo Scientific KingFisher consumables
Cat. No. Product Package size
97002070 KingFisher tip comb 50 pcs
97002080 KingFisher plate 100 µl 50 pcs
97002084 KingFisher plate 200 µl 50 pcs
97002090 KingFisher plastics 100 µl 8-pack, 8 plates and 8 tip combs 1 box
97002094 KingFisher plastics 200 µl 8-pack, 8 plates and 8 tip combs 1 box

Thermo Fisher Scientific10 Thermo Scientific KingFisher Plant DNA Kit Instruction Manual
Kit Content