Thermo Scientific myECL Imager User manual

myECL™Imager Manual
User Documentation

Table of Contents
Chapter 1: Overview.....................................................................................1
Thank You and Welcome Letter.............................................................1
Contacting Thermo Fisher Scientific...................................................2
Imager Overview ..........................................................................................2
Included Accessories ................................................................................2
Functionality.............................................................................................3
MYECL Imager Acquisition Software...........................................................3
myImageAnalysis Software ......................................................................4
Chapter 2: Imager...........................................................................................5
Installation Guidelines...............................................................................5
Space Requirements ................................................................................5
Electrical Requirements............................................................................5
Environmental Requirements....................................................................6
Computer Requirements...........................................................................6
Imager Images with Feature Locations .....................................................7
Start Up..................................................................................................10
Shut Down .............................................................................................11
USB Features................................................................................................12
Imager Settings...........................................................................................13
Tools ......................................................................................................13
Date and Time........................................................................................14
Printer ....................................................................................................15
Filters.....................................................................................................16
Administrator Settings ............................................................................18
Set Image Gallery Location .....................................................................19
Allow Remote Access .............................................................................19
Other Operating System (OS) Settings.....................................................21
Reset Default Focus Position...................................................................21
Update Software ...................................................................................21
Change Username and Password ...........................................................21
Create Dark and Bias Images .................................................................22
Return ....................................................................................................24

Chapter 3: Image Acquisition...........................................................25
Imager Settings.............................................................................................25
Live Camera ...........................................................................................25
Live Camera in Chemi Mode .................................................................25
Live Camera in UV Mode .....................................................................26
Live Camera in Vis Mode .....................................................................26
Camera Settings Available in Chemi, UV and Vis Modes ..........................27
Settings Only Available in UV and Vis Modes ...........................................29
Chemiluminescence (Chemi) Mode...................................................30
Camera Settings ...................................................................................30
Interactive Chemi ...................................................................................30
Capture Settings ...................................................................................33
Single Image Acquisition .....................................................................33
Pre-set Exposure Time .......................................................................34
Custom Exposure Time .......................................................................35
Multiple Image Acquisition ...................................................................36
Pre-set Multiple Exposure Settings ..........................................................37
User-defined Multiple Exposure Settings....................................................38
Image Tools ............................................................................................40
ChemiV Overlay ...............................................................................40
Display Saturation ............................................................................40
Invert Image...................................................................................41
Auto Contrast .................................................................................42
Adjust Image ..................................................................................42
Intensity Display ..............................................................................43
Zoom ..........................................................................................43
File Menu ...............................................................................................43
Image Info.....................................................................................44
Export..........................................................................................45
Print ...........................................................................................45
Delete .........................................................................................45

Ultraviolet (UV) Mode................................................................................46
Camera Settings.....................................................................................46
Capture Settings.....................................................................................46
Image Acquisition.............................................................................46
Pre-set Exposure Time .......................................................................47
Custom Exposure Time .......................................................................48
Stop Image Acquisition .......................................................................49
Image Tools ............................................................................................49
Display Saturation ............................................................................49
Invert Image...................................................................................50
Auto Contrast .................................................................................50
Adjust Image ..................................................................................50
Intensity Display ..............................................................................51
Zoom ..........................................................................................51
File Menu ...............................................................................................51
Image Info.....................................................................................51
Export ........................................................................................51
Print ...........................................................................................51
Delete .........................................................................................51
Visible (Vis) Mode ......................................................................................52
Camera Settings.....................................................................................52
Image Acquisition–Transmitted Light........................................................52
Image Acquisition–Reflected Light...........................................................53
Pre-set Exposure Time .......................................................................53
Custom Exposure Time .......................................................................54
Image Tools ............................................................................................54
Display Saturation ............................................................................55
Invert Image...................................................................................55
Auto Contrast .................................................................................56
Adjust Image ..................................................................................56
Intensity Display ..............................................................................56
Zoom ..........................................................................................56
File Menu ...............................................................................................56
Image Info.....................................................................................56
Export ........................................................................................56
Print ...........................................................................................57
Delete .........................................................................................57

Image Gallery...............................................................................................57
Acquisition Information...........................................................................57
Image Viewing ................................................................................58
Thumbnail.....................................................................................58
File List ..................................................................................................59
Search....................................................................................................59
Search Results .......................................................................................60
File Manager ..........................................................................................60
Copy ...........................................................................................60
Delete..........................................................................................60
Export..........................................................................................61
Edit Info........................................................................................61
Unselect .......................................................................................61
Exit File Manager .............................................................................61
Chapter 4: Maintenance.........................................................................62
Cleaning the Imager and Sample Screens .....................................62
LCD Touchscreen........................................................................................62
Use of the Stylus.........................................................................................62
UV Transilluminator...................................................................................63
Band Excision.........................................................................................63
Cleaning the Transilluminator..................................................................64
Replacement of Transilluminator Bulbs ...................................................64
Emission Filters ..........................................................................................65
Changing Filters .....................................................................................65
Appendix A: Technical Specifications......................................67
Appendix B: Accessories.......................................................................68
Appendix C: Related Thermo Scientific Products........69
Glossary..................................................................................................................70

1MYECL Imager User Manual Thermo Fisher Scientific
Thank You and Welcome
Chapter 1 Overview
Dear Researcher:
We thank you for your investment in our Thermo Scientific™MYECL™Imager. We
have worked to design a highly functional, yet intuitive, imager that will provide you
with valuable images and data of high-resolution and quality.
This manual has been written as a concise guide for the start-up, use and
customization of your MYECL Imager unit and acquisition software. Detailed sections
covering the imager, image acquisition, file management, maintenance and
troubleshooting provide comprehensive information. We encourage you to read the
entire manual for a full understanding of the imager, but a Quick Start guide is
provided if you want to begin using your new imager immediately.
Thermo Scientific products are always at the forefront of scientific instrument
design, and we strive to design and produce instruments that can help you achieve
your research goals. Your feedback is a valuable tool that we use for future product
development, so please contact us if you have any questions, comments or
suggestions.
IMPORTANT Review and implement guidelines for proper set-up before installation.
Locate the imager away from water, solvents, corrosive materials, strong magnetic
fields and vibration sources.
IMPORTANT The imager weighs 65lbs (29.5kg). Please use two people to lift it by
grasping the bottom and the handle at the rear of the imager. Using two people,
place the imager onto the benchtop given proper space, electrical and environmental
requirements. Remove the outer packaging materials.
IMPORTANT Imager use outside of the workflows described in this manual may put
the operator at risk of dangerous exposure to electrical shock or radiated emissions.
Do not use this instrument for any purposes or in any configurations not described in
this manual.

2MYECL Imager User Manual Thermo Fisher Scientific
Contacting Us
Imager Overview
Included Accessories
For technical or service assistance on the MYECL Imager:
U.S. Customers: 1-800-874-3723
International Customers: 1-815-968-0747
European Customers: [phone]
Asia/Pacific Customers: [phone]
Technical Support email for U.S. and international customers:
[e-mail]
Technical Support email for European customers:
[e-mail]
Technical Support email for Asia/Pacific customers:
[email protected]om
For more information about the Thermo Scientific Protein Biology product line,
please visit our website: www.thermoscientific.com/pierce
The MYECL Imager is designed to be a useful tool for the researcher needing intuitive
equipment and software with fast imaging capabilities.
Your imager purchase includes the following accessories (each quantity 1):
Thermo Scientific™MYImageAnalysis™Software
MYECL Imager User Manual CD
Chemiluminescence exposure screen
White light conversion screen
UV exposure screen
Imaging reference target
Quick start guide
USB memory device
Stylus
Stylus holder
Power cord with C13 connector
Optional items, including additional filters and UV bulbs (365nm), may be added to
the above list depending on the items ordered with the imager.
Note Upon receiving your imager, please verify you have received all of the items
listed above. Please contact Thermo Scientific Technical Support for assistance.

3MYECL Imager User Manual Thermo Fisher Scientific
Functionality
Thermo Scientific
MYECL Imager
Acquisition Software
The MYECL Imager provides:
UV and white light illumination source for imaging of samples
Acquisition of chemiluminescent Western blot and electrophoresis gel images
with varying resolution (1×1, 2×2, 3×3, 4×4 and 8×8 binning)
Low-noise, high-dynamic range CCD camera detection
Hardware and software optimized for image acquisition
One-touch image acquisition
Multiple image acquisition with pre-set and user-defined settings
Each imager arrives loaded with the MYECL Imager Acquisition Software. This
manual gives detailed information on using all software functions and modes.
The acquisition software allows you to acquire images using three different modes:
Chemiluminescence (Chemi) mode for image acquisition of self-emitting,
chemiluminescent light
Ultraviolet (UV) mode for image acquisition of UV-transmitted light
Visible (Vis) mode for image acquisition of reflected and/or transmitted light

4MYECL Imager User Manual Thermo Fisher Scientific
Thermo Scientific
MYImageAnalysis
Software
The MYImageAnalysis Software is supplied with the MYECL Imager as a vital tool for
extracting information from your captured images. The analysis software features:
Indentify lanes and bands automatically
View and compare up to six images simultaneously
Open multiple image formats (e.g., .tif, .jpeg, .png, etc.)
Export images and data reports directly to Microsoft™Excel™, Word™and
PowerPoint™software for further analysis and presentations
Analyze molecular weight –determine the Rfand molecular weight of protein or
nucleic acid bands using installed or custom added molecular weight markers
Edit digital images –image editing tools include contrast adjustment, image
rotation, cropping, de-speckling, false color application and text annotation
Calculate sample purity automatically based on band and lane intensity
Perform lane profile densitometry
Quantitate bands or regions relatively or absolutely
Acquired images are saved and exported as .tif files, which can be directly opened in
the analysis software.

5MYECL Imager User Manual Thermo Fisher Scientific
Installation
Guidelines
Space Requirements
Electrical Requirements
Chapter 2 Imager
IMPORTANT Review and implement guidelines for proper set-up before installation.
Locate the imager away from water, solvents, corrosive materials, strong magnetic
fields and vibration sources.
IMPORTANT The imager weighs 65lbs (29.5kg). Use two people to lift it by grasping
the bottom and the handle at the rear of the imager. Using two people, place the
imager onto the benchtop given proper space, electrical and environmental
requirements. Remove the outer and inner packaging materials.
The imager requires a stable laboratory bench or table capable of supporting 65lbs
(29.5kg). Provide a minimum clearance of 3 inches (7.62cm) at the rear of the
imager to allow adequate ventilation for camera cooling and access to the power
cord and switch. In addition, allow sufficient space to open the front-facing imager
door to place sample within the imager. Instrument dimensions are listed in
Table 2-1.
Table 2-1. Imager dimensions.
Dimensions
Inches
Centimeters
Width
12.25
31.1
Depth
22
55.9
Height
21
53.3
A grounded circuit capable of delivering the appropriate current and voltage is
required for installation. Electrical requirements can be located on the imager label
on the rear panel of the imager and in Table 2-2. The imager electrical system
adjusts to the proper voltage for the respective country (Table 2-3). Connect the
imager power cord to the rear left side panel of the system and plug into a grounded
power outlet.