Thermo Scientific myECL Imager User manual

myECL™Imager Manual
User Documentation

Table of Contents
Chapter 1: Overview.....................................................................................1
Thank You and Welcome Letter.............................................................1
Contacting Thermo Fisher Scientific...................................................2
Imager Overview ..........................................................................................2
Included Accessories ................................................................................2
Functionality.............................................................................................3
MYECL Imager Acquisition Software...........................................................3
myImageAnalysis Software ......................................................................4
Chapter 2: Imager...........................................................................................5
Installation Guidelines...............................................................................5
Space Requirements ................................................................................5
Electrical Requirements............................................................................5
Environmental Requirements....................................................................6
Computer Requirements...........................................................................6
Imager Images with Feature Locations .....................................................7
Start Up..................................................................................................10
Shut Down .............................................................................................11
USB Features................................................................................................12
Imager Settings...........................................................................................13
Tools ......................................................................................................13
Date and Time........................................................................................14
Printer ....................................................................................................15
Filters.....................................................................................................16
Administrator Settings ............................................................................18
Set Image Gallery Location .....................................................................19
Allow Remote Access .............................................................................19
Other Operating System (OS) Settings.....................................................21
Reset Default Focus Position...................................................................21
Update Software ...................................................................................21
Change Username and Password ...........................................................21
Create Dark and Bias Images .................................................................22
Return ....................................................................................................24

Chapter 3: Image Acquisition...........................................................25
Imager Settings.............................................................................................25
Live Camera ...........................................................................................25
Live Camera in Chemi Mode .................................................................25
Live Camera in UV Mode .....................................................................26
Live Camera in Vis Mode .....................................................................26
Camera Settings Available in Chemi, UV and Vis Modes ..........................27
Settings Only Available in UV and Vis Modes ...........................................29
Chemiluminescence (Chemi) Mode...................................................30
Camera Settings ...................................................................................30
Interactive Chemi ...................................................................................30
Capture Settings ...................................................................................33
Single Image Acquisition .....................................................................33
Pre-set Exposure Time .......................................................................34
Custom Exposure Time .......................................................................35
Multiple Image Acquisition ...................................................................36
Pre-set Multiple Exposure Settings ..........................................................37
User-defined Multiple Exposure Settings....................................................38
Image Tools ............................................................................................40
ChemiV Overlay ...............................................................................40
Display Saturation ............................................................................40
Invert Image...................................................................................41
Auto Contrast .................................................................................42
Adjust Image ..................................................................................42
Intensity Display ..............................................................................43
Zoom ..........................................................................................43
File Menu ...............................................................................................43
Image Info.....................................................................................44
Export..........................................................................................45
Print ...........................................................................................45
Delete .........................................................................................45

Ultraviolet (UV) Mode................................................................................46
Camera Settings.....................................................................................46
Capture Settings.....................................................................................46
Image Acquisition.............................................................................46
Pre-set Exposure Time .......................................................................47
Custom Exposure Time .......................................................................48
Stop Image Acquisition .......................................................................49
Image Tools ............................................................................................49
Display Saturation ............................................................................49
Invert Image...................................................................................50
Auto Contrast .................................................................................50
Adjust Image ..................................................................................50
Intensity Display ..............................................................................51
Zoom ..........................................................................................51
File Menu ...............................................................................................51
Image Info.....................................................................................51
Export ........................................................................................51
Print ...........................................................................................51
Delete .........................................................................................51
Visible (Vis) Mode ......................................................................................52
Camera Settings.....................................................................................52
Image Acquisition–Transmitted Light........................................................52
Image Acquisition–Reflected Light...........................................................53
Pre-set Exposure Time .......................................................................53
Custom Exposure Time .......................................................................54
Image Tools ............................................................................................54
Display Saturation ............................................................................55
Invert Image...................................................................................55
Auto Contrast .................................................................................56
Adjust Image ..................................................................................56
Intensity Display ..............................................................................56
Zoom ..........................................................................................56
File Menu ...............................................................................................56
Image Info.....................................................................................56
Export ........................................................................................56
Print ...........................................................................................57
Delete .........................................................................................57

Image Gallery...............................................................................................57
Acquisition Information...........................................................................57
Image Viewing ................................................................................58
Thumbnail.....................................................................................58
File List ..................................................................................................59
Search....................................................................................................59
Search Results .......................................................................................60
File Manager ..........................................................................................60
Copy ...........................................................................................60
Delete..........................................................................................60
Export..........................................................................................61
Edit Info........................................................................................61
Unselect .......................................................................................61
Exit File Manager .............................................................................61
Chapter 4: Maintenance.........................................................................62
Cleaning the Imager and Sample Screens .....................................62
LCD Touchscreen........................................................................................62
Use of the Stylus.........................................................................................62
UV Transilluminator...................................................................................63
Band Excision.........................................................................................63
Cleaning the Transilluminator..................................................................64
Replacement of Transilluminator Bulbs ...................................................64
Emission Filters ..........................................................................................65
Changing Filters .....................................................................................65
Appendix A: Technical Specifications......................................67
Appendix B: Accessories.......................................................................68
Appendix C: Related Thermo Scientific Products........69
Glossary..................................................................................................................70

1MYECL Imager User Manual Thermo Fisher Scientific
Thank You and Welcome
Chapter 1 Overview
Dear Researcher:
We thank you for your investment in our Thermo Scientific™MYECL™Imager. We
have worked to design a highly functional, yet intuitive, imager that will provide you
with valuable images and data of high-resolution and quality.
This manual has been written as a concise guide for the start-up, use and
customization of your MYECL Imager unit and acquisition software. Detailed sections
covering the imager, image acquisition, file management, maintenance and
troubleshooting provide comprehensive information. We encourage you to read the
entire manual for a full understanding of the imager, but a Quick Start guide is
provided if you want to begin using your new imager immediately.
Thermo Scientific products are always at the forefront of scientific instrument
design, and we strive to design and produce instruments that can help you achieve
your research goals. Your feedback is a valuable tool that we use for future product
development, so please contact us if you have any questions, comments or
suggestions.
IMPORTANT Review and implement guidelines for proper set-up before installation.
Locate the imager away from water, solvents, corrosive materials, strong magnetic
fields and vibration sources.
IMPORTANT The imager weighs 65lbs (29.5kg). Please use two people to lift it by
grasping the bottom and the handle at the rear of the imager. Using two people,
place the imager onto the benchtop given proper space, electrical and environmental
requirements. Remove the outer packaging materials.
IMPORTANT Imager use outside of the workflows described in this manual may put
the operator at risk of dangerous exposure to electrical shock or radiated emissions.
Do not use this instrument for any purposes or in any configurations not described in
this manual.

2MYECL Imager User Manual Thermo Fisher Scientific
Contacting Us
Imager Overview
Included Accessories
For technical or service assistance on the MYECL Imager:
U.S. Customers: 1-800-874-3723
International Customers: 1-815-968-0747
European Customers: [phone]
Asia/Pacific Customers: [phone]
Technical Support email for U.S. and international customers:
[e-mail]
Technical Support email for European customers:
[e-mail]
Technical Support email for Asia/Pacific customers:
[email protected]om
For more information about the Thermo Scientific Protein Biology product line,
please visit our website: www.thermoscientific.com/pierce
The MYECL Imager is designed to be a useful tool for the researcher needing intuitive
equipment and software with fast imaging capabilities.
Your imager purchase includes the following accessories (each quantity 1):
Thermo Scientific™MYImageAnalysis™Software
MYECL Imager User Manual CD
Chemiluminescence exposure screen
White light conversion screen
UV exposure screen
Imaging reference target
Quick start guide
USB memory device
Stylus
Stylus holder
Power cord with C13 connector
Optional items, including additional filters and UV bulbs (365nm), may be added to
the above list depending on the items ordered with the imager.
Note Upon receiving your imager, please verify you have received all of the items
listed above. Please contact Thermo Scientific Technical Support for assistance.

3MYECL Imager User Manual Thermo Fisher Scientific
Functionality
Thermo Scientific
MYECL Imager
Acquisition Software
The MYECL Imager provides:
UV and white light illumination source for imaging of samples
Acquisition of chemiluminescent Western blot and electrophoresis gel images
with varying resolution (1×1, 2×2, 3×3, 4×4 and 8×8 binning)
Low-noise, high-dynamic range CCD camera detection
Hardware and software optimized for image acquisition
One-touch image acquisition
Multiple image acquisition with pre-set and user-defined settings
Each imager arrives loaded with the MYECL Imager Acquisition Software. This
manual gives detailed information on using all software functions and modes.
The acquisition software allows you to acquire images using three different modes:
Chemiluminescence (Chemi) mode for image acquisition of self-emitting,
chemiluminescent light
Ultraviolet (UV) mode for image acquisition of UV-transmitted light
Visible (Vis) mode for image acquisition of reflected and/or transmitted light

4MYECL Imager User Manual Thermo Fisher Scientific
Thermo Scientific
MYImageAnalysis
Software
The MYImageAnalysis Software is supplied with the MYECL Imager as a vital tool for
extracting information from your captured images. The analysis software features:
Indentify lanes and bands automatically
View and compare up to six images simultaneously
Open multiple image formats (e.g., .tif, .jpeg, .png, etc.)
Export images and data reports directly to Microsoft™Excel™, Word™and
PowerPoint™software for further analysis and presentations
Analyze molecular weight –determine the Rfand molecular weight of protein or
nucleic acid bands using installed or custom added molecular weight markers
Edit digital images –image editing tools include contrast adjustment, image
rotation, cropping, de-speckling, false color application and text annotation
Calculate sample purity automatically based on band and lane intensity
Perform lane profile densitometry
Quantitate bands or regions relatively or absolutely
Acquired images are saved and exported as .tif files, which can be directly opened in
the analysis software.

5MYECL Imager User Manual Thermo Fisher Scientific
Installation
Guidelines
Space Requirements
Electrical Requirements
Chapter 2 Imager
IMPORTANT Review and implement guidelines for proper set-up before installation.
Locate the imager away from water, solvents, corrosive materials, strong magnetic
fields and vibration sources.
IMPORTANT The imager weighs 65lbs (29.5kg). Use two people to lift it by grasping
the bottom and the handle at the rear of the imager. Using two people, place the
imager onto the benchtop given proper space, electrical and environmental
requirements. Remove the outer and inner packaging materials.
The imager requires a stable laboratory bench or table capable of supporting 65lbs
(29.5kg). Provide a minimum clearance of 3 inches (7.62cm) at the rear of the
imager to allow adequate ventilation for camera cooling and access to the power
cord and switch. In addition, allow sufficient space to open the front-facing imager
door to place sample within the imager. Instrument dimensions are listed in
Table 2-1.
Table 2-1. Imager dimensions.
Dimensions
Inches
Centimeters
Width
12.25
31.1
Depth
22
55.9
Height
21
53.3
A grounded circuit capable of delivering the appropriate current and voltage is
required for installation. Electrical requirements can be located on the imager label
on the rear panel of the imager and in Table 2-2. The imager electrical system
adjusts to the proper voltage for the respective country (Table 2-3). Connect the
imager power cord to the rear left side panel of the system and plug into a grounded
power outlet.

6MYECL Imager User Manual Thermo Fisher Scientific
Environmental
Requirements
Computer Requirements
Table 2-2. Imager electrical ratings.
Electrical Parameter
Rating
Supply Voltage (VAC)
120-240
Frequency (Hz)
50-60
Power Rating (W)
120
Fuse (power center)
T4AL, 250V, 4A
Table 2-3. Imager electrical requirements for major countries.
Region
Voltage
(VAC)
Frequency
(Hz)
Amps (A)
US and Canada
120
60
1.0
Europe
220
50
0.5
UK
240
50
0.5
Japan
100
50
1.2
China
220
50
0.5
India
230
50
0.5
South Korea
220
60
0.5
For optimal performance, maintain a constant laboratory temperature range of 15-
25°C (59-77°F) with relative humidity between 15-70%. To allow adequate camera
cooling, do not place the imager in direct sunlight.
The imager is operated by an integrated computer, which is accessed through the
touchscreen.
The MYImageAnalysis Software program is included with the imager purchase. To
run the MYImageAnalysis Software properly, workstations must meet the minimum
performance requirements listed in Table 2-4.
Table 2-4. Recommended computer requirements for
MYImageAnalysis Software.
Description
Recommended
Operating System
Windows 7, Vista*, or XP*
Processor
Pentium* 4 @ 2.4GHz
Memory (RAM)
512MB
Hard Drive
2GB
Screen Resolution
1280 × 1024†
†Refer to the MYImageAnalysis Software User Manual for more information.

7MYECL Imager User Manual Thermo Fisher Scientific
Imager Images with
Feature Locations
Imager Front View
Figure 2-1. Imager front and side views detailing main components.

8MYECL Imager User Manual Thermo Fisher Scientific
Imager Front View with
Sample Door Open
Figure 2-2. Imager view with the sample door open and the transilluminator
extended.

9MYECL Imager User Manual Thermo Fisher Scientific
Imager Rear View
Figure 2-3. Rear imager view.

10 MYECL Imager User Manual Thermo Fisher Scientific
Start Up
Hard Start Up
IMPORTANT Allow the imager to equilibrate for 20-30 minutes after each initial
hard power up. This ensures that the camera is optimally cooled before image
acquisition.
To perform the Hard Start Up procedure:
1. Turn on the hard power switch located at the bottom of the rear imager panel.
The hard power switch provides power to all of the imager components.
2. Turn on the soft power switch on the front of the imager. This switch activates
the internal computer, touchscreen and thermoelectric camera cooler.
3. The computer boots up.
4. The user is prompted to enter the current date, time and time zone. Upon
completion, the screen automatically moves to the default Chemi acquire
screen.

11 MYECL Imager User Manual Thermo Fisher Scientific
Soft Start Up
Shut Down
Soft Shut Down
Hard Shut Down
If the imager is powered down by the Soft Shut Down procedure, then a Soft Start
Up can be done using the front, soft power switch. The soft power switch is the blue
lighted switch located just above the front door and below the touchscreen. This
switch allows the user power down the imager computer and touchscreen, while
maintaining power to the camera cooling unit. This feature allows the user to power
down the imager, but, once needed, quickly restart the imager without having to
wait for the camera temperature to re-equilibrate.
1. Turn on the front soft power switch. The computer restarts and the touchscreen
initiates with the default Chemi screen.
2. Begin normal operation of the imager.
Note For extended periods of inactivity, the imager can be hard powered off;
however, for typical daily use, optimal camera temperature, and best image quality,
avoid hard powering on and off the imager.
The Soft Shut Down procedure uses only the front soft power switch.
1. Save any current image analyses.
2. Safely remove any USB flash drives.
3. Turn off the soft power switch.
4. The computer and touchscreen shut down and turn off. The fans continue
running as needed. The camera cooler remains powered and continue to
maintain the camera at the optimal -25°C.
5. The transilluminator and epi-white lights turn off.
The Hard Shut Down procedure uses the hard power switch on the rear of the
imager. This turns off all power to the imager, including the computer and camera
cooler.
1. Follow the Soft Shut Down procedure.
2. Turn off the hard power switch at the rear of the imager.

12 MYECL Imager User Manual Thermo Fisher Scientific
USB Features
The three external USB ports on the right side of the imager can be used for flash
drives, which can be connected through any of the USB ports for easy file
management.
Note The Export button always exports files to the E:\ drive, which is automatically
the first additional drive connected to the imager. When connecting an external hard
drive to the imager, assign the external drive to a letter other than C, D or E.
Figure 2-3. Location of upper USB port.
Figure 2-4. Location of lower USB ports.

13 MYECL Imager User Manual Thermo Fisher Scientific
Imager Settings
Tools
The Tools button in the top right corner of the acquisition software screen allows
access to basic imager settings and functionality.
1. Select the Tools button to open the Tools screen.
The Tools screen displays the serial number of the system and the current software
version installed in the system. Large buttons for Date and Time, Printer, Filters,and
Administrator Settings are displayed on the screen. Select the appropriate button to
enter setup for each item.
Tools button

14 MYECL Imager User Manual Thermo Fisher Scientific
Date and Time
To correct the date, time or time zone:
1. Select the Date and Time button. A Windows clock and calendar is displayed.
2. Choose the correct settings as needed.
3. Select the Apply button, followed by the OK button to save the settings.

15 MYECL Imager User Manual Thermo Fisher Scientific
Printer
To select a default printer for imager exporting:
1. Select the Printer button. A Printers and Other Devices screen is displayed.
2. Choose the appropriate printer from the listed selections. Plug-and-play printers
automatically populate the list. Select Add a Printer from the left column if the printer
is not a local printer or plug-and-play type. A network printer can also be selected as
a default printer.
Note Consult your facility’s IT department for assistance in setting up network
printers.