VWR Peqlab peqPETTE eType User manual

Instruction Manual / Bedienungsanleitung
peqPETTE eType
Electronic pipettes / Elektronische Pipetten
v0314_E+D Creating the future
t
ogethe
r
.

Instruction Manual peqPETTE eType
PEQLAB_v0314_E
CONTENTS
1SAFETY PRECAUTIONS 1
1.1 Operation Safety Precautions 1
1.2 Battery Safety 1
2GENERAL DESCRIPTION 2
2.1 Features 2
2.2 Ranges and Specifications 2
2.3 Technical data 3
2.4 Warranty 3
3GETTING STARTED 4
3.1 Unpacking the peqPETTE eType 4
3.2 Battery installation guide 4
3.3 Charging the Battery 5
3.4 Start Pipetting 5
3.5 Manual Blow-out Operation 6
3.6 Hanger Installation 6
3.7 Tip Ejector Height Adjustment 7
4PEQPETTE ETYPE PIPETTE OVERVIEW 8
5FUNCTION MAP 10
6SYS (SYSTEM) MODE 10
6.1 Aspirating Speed 10
6.2 Dispensing Speed 11
6.3 Audible Signals 11
7BLOW-OUT 11
7.1 Reverse Pipetting 11
7.2 Setting PROG (Programs) 12
8AUTO MODE 12
8.1 AUTO Mode Only 12
8.2 AUTO + MIX Mode 13
9MD (MULTIPLE DISPENSING) MODE 13
10 MIX MODE 14
11 SE (SEQUENTIAL ASPIRATING/DISPENSING) MODE 14
11.1 SA (Sequential Aspirating) Mode 14
11.2 SD (Sequential Dispensing) Mode 15
12 STERILIZATION 16
12.1 Removal of the Lower Piston Assembly 16

Instruction Manual peqPETTE eType
PEQLAB_v0314_E
12.2 Reattach the Lower Piston Assembly 17
13 MAINTENANCE 17
14 TECHNICAL SERVICE AND ODERING INFORMATION 18
15 TROUBLESHOOTING 19

Instruction Manual peqPETTE eType
PEQLAB_v0314_E 1
1SAFETY PRECAUTIONS
Before using the peqPETTE eType electronic pipette for the first time, please read this entire operating
manual carefully and keep this instruction manual for future reference. To guarantee problem free, safe
operation, it is essential to observe the following points:
1.1 Operation Safety Precautions
When using infectious, radioactive, toxic and other solutions, which may pose health risks, please
observe appropriate safety precautions.
Do not use the peqPETTE eType electronic pipette in a potentially explosive environment or with
potentially explosive chemicals.
When using organic solvents or corrosive chemicals, please check their compatibility with the pipette
tips and the peqPETTE eType electronic pipette.
Repair should be carried out by PEQLAB’s authorized service personnel only.
Contact PEQLAB for information on authorized service options.
Use original spare parts and accessories only.
1.2 Battery Safety
The peqPETTE eType electronic pipettes operate on a small but powerful Lithium-ion battery. Misuse or
abuse of the Lithium-ion battery may cause damage or injury through fire, electric shock, or chemical
leakage. Please read and understand all warnings before using the battery.
1. When storing the battery, do not allow it to come into contact with any metallic surfaces.
2. Do not incinerate the Lithium battery or expose it to excessive heat.
3. Do not short-circuit, puncture, crush, disassemble, damage, force over-discharge (reversal) or
modify the battery.
4. Do not expose the battery to water or moisture.
5. Do not drop or subject the battery to strong impacts.
6. Only use the battery specified in this manual.
7. Only use the specified battery charging unit or DC-in jack to charge the battery.
8. Do not use a leaking battery.
9. If charging is not completed within the specified time period, unplug the charger and
discontinue charging immediately.
10. The charger and battery temperature rises with extended periods of use. Care should be taken
to avoid burns.
11. Burns may result if the battery is removed immediately after extended periods of use.
12. If fluid from the battery enters your eye, immediately rinse the eye with plenty of fresh water and
contact a doctor. If fluid from the battery makes contact with your skin or clothing, wash the
area thoroughly with water.

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PEQLAB_v0314_E 2
2GENERAL DESCRIPTION
peqPETTE eType electronic pipettes feature an accurate and efficient, as well as ergonomic and light-
weight design. Researchers can program these state-of-the-art products to conduct different pipetting
tasks. More accurate and comfortable pipetting can be easily achieved without any concern of finger
stress or injury.
2.1 Features
High accuracy and precision
Low forces
Useful, practical operation protocols, such as Automatic Pipetting (AUTO) with Mixing (MIX)
option, Multiple Dispensing (MD), Mixing (MIX) and Sequential Aspirating/Dispensing (SE), etc.
9 user-defined program sets
5 speeds for aspirating and dispensing
Li-ion battery provides extended use on a single battery charge
Ergonomic design
Automatic calibration
User-friendly graphic operation interface
Adjustable tip ejector
Automatically remembers the last-used pipetting protocol and settings
Complies with CE, ISO-8655, GLP
2.2 Ranges and Specifications
Product No. Channels Volume Range
(μl)
Increment
(μl)
Accuracy
(Rel.±)
Precision
(Rel. CV )
91-PEM-10EU/-UK/-US 8 0.5-10 0.1 4.0-1.5% 2.5-0.5%
91-PEM-20EU/-UK/-US 8 2-20 0.1 4.0-1.0% 2.0-0.5%
91-PEM-200EU/-UK/-UK 8 10-200 1 3.0-0.6% 1.0-0.15%
91-PEM-1200EU/-UK/-US 8 100 -1,200 1 3.0-0.5% 0.8-0.2%
Note: peqPETTE eType electronic pipette Accuracy and Precision Specifications have been attained using PEQLAB
pipette tips. PEQLAB guarantees accuracy and precision only if PEQLAB tips or other approved tips are used.

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2.3 Technical data
Operation Mode Automatic Pipetting (AUTO) with Mixing option,
Multiple Dispensing (MD),
Mixing(MIX),
Sequential Aspirating/Dispensing (SE),
System Setup (SYS)
Program memory 9 programs
Aspirating/Dispensing Speed 5 speeds
Auto-calibration Yes
Piston Motor High precision stepping motor
Power Saving Feature Yes, after 10 minutes
Power Adaptor 100/120/220/240 V AC-DC 5 V
Audible Alerts Yes
Autoclavable Yes, lower manifold only
Operating Temperature 5 ~ 50oC
Operating Humidity RH: 0 ~ 85%
Battery 920 mAh/3.6 V or above, Li-Ion
Carrousel Stand Optional
Certification Complies with CE, Class A (EN60101-1-2,
EN50082-1, EN 55011)
Complies with ISO-8655/ DIN 12650
Note: Specifications are subject to change without prior notice
2.4 Warranty
PEQLAB guarantees that the peqPETTE eType electronic pipette you have received has been thoroughly
tested and meets its published specification.
However, immediately upon arrival, please check carefully that the shipment is complete and has not
been damaged in transit. For missing parts or to report any kind of damage, please contact PEQLAB
(see 'TECHNICAL SUPPORT’). Please retain all packaging materials until the delivery has been
completely checked since this will speed up the return of goods if required and reduce environmental
impact. Any form of returns, replacements or credit notes must be agreed in advance by PEQLAB.
The peqPETTE eType has been subjected to thorough testing and quality control. In the unlikely event of
a manufacturing fault, our two year warranty (from the date of delivery) covers the peqPETTE eType.
This warranty becomes invalid in the case of incorrect operation, use of non-standard spare parts or
accessories and unauthorized modifications. The warranty does not cover defects caused by excessive
wear and tear or damage due to shipping, accident, abuse, misuse, problems with electrical power, or
usage not in accordance with product instructions, or if other than original spare parts supplied by the
manufacturer have been used. This warranty does not apply if the product has been damaged by
accident, abuse, misuse, or misapplication, or from ordinary wear and tear.
PEQLAB reserves the right to make technical modifications. For your protection, items being returned
must be insured against possible damage or loss. This warranty shall be limited to the replacement of
defective products. For research use only. Not intended for any animal or human therapeutic or
diagnostic use.

Instruction Manual peqPETTE eType
PEQLAB_v0314_E 4
3GETTING STARTED
3.1 Unpacking the peqPETTE eType
Open the peqPETTE eType electronic pipette package and confirm that all items are included:
-Electronic pipette unit
-Wall hanger and accessories
-Battery
-AC-DC power adaptor
-Operation manual
Note:
1. If there are any items missing, damaged, or not according to your order, please contact your distributor or sales
representatives for replacement immediately.
2. Please charge the battery before first-time operation.
3.2 Battery installation guide
Remove the battery cover by pressing the battery cover latch (see Figure 1) and move the cover
outward. Plug in the battery connector and insert the battery (see Figure 2). Please consider the notes
regarding correct connection and correct wire arrangement shown in figure 3 and 4.
Close the cover of the battery compartment. Press the RESET button to power on and the peqPETTE
eType electronic pipette will automatically carry out a calibration routine.
Fi
g
ure 1: O
p
en the batter
y
cove
r
.
Batter
y
(1)
(2)
(2) Battery
W
ire
(1) Connector
Fi
g
ure 2: Insert the batter
y
Bad connection! The connector isn’t
pushed into the socket completely. Correct connection!
Fi
g
ure 3: Connecto
r

Instruction Manual peqPETTE eType
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Note: After a charged battery is inserted, the peqPETTE eType electronic pipette will automatically carry out a
calibration routine.
3.3 Charging the Battery
Attach the included AC-DC Power Adapter to the Pipette to charge the battery.
Insert the battery into the battery compartment. Insert the DC-in plug of the power adaptor into the DC-
in jack (see Figure 5). Connect the power plug of the power adaptor with the external power source.
Connecting the power plug before inserting the battery will inactivate the charging process. The
indication bar inside battery symbol will blink during the charging process. When the battery is
fully charged ( ), the blinking will stop and the peqPETTE eType electronic pipette will beep once to
remind the user.
Note:
1.
peqPETTE eType electronic pipettes can be used during charging.
2.
Please do not over-charge the battery for a long period of time.
3.
Do not insert or remove the battery during the charging process, it may cause battery or PCBA damage.
3.4 Start Pipetting
There is no power On/Off button on the peqPETTE eType electronic pipettes. Once users insert the fully-
charged battery, the pipette will be switched on. It will perform a calibration routine and switch on the
LCD display as in Figure 10. Press any keypad button or the PLUNGER button to enter the function
mode selection. The peqPETTE eType electronic pipette will be automatically powered off and enter
sleeping mode, if it’s not in use for more than 10 minutes.
Note: In sleeping mode, if the DC-in plug of power adaptor is connected to the pipette, the LCD display will show
battery charging symbol. On the contrast, the LCD display will show nothing.
Figure 5: Using the
included AC-DC adaptor
to charge the battery.
Ba
d
arrangement! Wire is presse
d
b
y
the batter
y
Correct wire arrangement!
Fi
g
ure 4: Wire arran
g
ement

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Select the desired function mode and settings (refer to the following chapters: 6 - 11) before pipetting.
After the initial set-up, attach the correct-sized tips to the cones of the pipette before operation.
Product No. Channels Volume Range (μl) Correct Pipette Tip Size
91-PEM-10EU/-UK/-US 8 0.5 -10 10μl
91-PEM-20EU/-UK/-US 8 2-20 10μl
91-PEM-200EU/-UK/-US 8 10-200 200 μl
91-PEM-1200EU/-UK/-US 8 100 -1,200 1,000/1,200 μl
Note: Some 1,000μl tips have enough space to hold up to 1,200μl of sample. Please check manufacturer’s data.
The cones of the peqPETTE eType electronic pipette are designed to fit a wide variety of manufacturers
pipette tips. However, the published accuracy and precision specifications for the peqPETTE eType
electronic pipettes are guaranteed only when using PEQLAB tips. Contact PEQLAB for pipette tip
ordering information.
3.5 Manual Blow-out Operation
The automatic blow-out function, in different pipetting modes, simulates the blow-out function in manual
pipettes. With the Blow-out function selected, the user can press the PLUNGER button once to dispense
the liquid and the piston will travel an extra distance to blow out any remaining liquid. The piston then
will pause and return to its starting position. Alternatively the user can press and hold the PLUNGER
button to dispense. The piston will travel the extra distance to blow out remaining liquid and remain in
that position until the plunger is released. Only when the PLUNGER button is released, the piston will
return back to its starting position.
3.6 Hanger Installation
The peqPETTE eType electronic pipette hanger, which is included with the delivery scope, can be
attached to a shelf or counter using the sticker as shown in Figure 7. The finger rest of the peqPETTE
eType electronic pipette locks into the hanger and keeps the pipette in a vertical position.
Fi
g
ure 6: Correct hand
p
osition.
Figure 7: peqPETTE eType electronic
pipette hanger installation.

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3.7 Tip Ejector Height Adjustment
Use a flat-head screw driver or other tool to turn the tip ejector height adjustment screw as shown in
Figure 8. By turning the screw in a counter-clockwise direction, the tip ejector can be adjusted down.
This height adjustment feature enables efficient ejecting of different manufacturer’s tips.
Figure 8: Tip ejector height adjustment.
Flat-head screw

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4PEQPETTE ETYPE PIPETTE OVERVIEW
This section presents an overview of the peqPETTE eType electronic pipette’s various components and
buttons (see Figure 9) as well as the symbols and indicators on the LCD display (see Figure 10).
LCD Dis
p
la
y
“RESET” Button
Ke
yp
ad
Ti
p
E
j
ecto
r
Bushin
g
Lower Par
t
Ti
p
Fittin
g
s
Connector Nu
t
Finger Res
t
PLUNGER Button
DC In Jack
Batter
y
Cove
r
Fi
g
ure 9: 8-channel
p
e
q
PETTE e
T
yp
e electronic
p
i
p
ette overview.
(Front View) (Back View)

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Keypad Function
Button Symbol Function Description
ENTER Confirms your choice
BACK Exits to previous mode/setting
Up Increases volume/ changes parameter
Down Decreases volume/ changes parameter
Reset RESET Piston automatically determines the home position
PLUNGER PLUNGER Activates aspirating/dispensing/ same as button1
Tip Ejector TIP Ejects the tips
1
Tips for Efficient Operation: The PLUNGER button can be used to increase volumes and to change modes and
settings same as the “Up” button during the function mode selection and volume settings. This operation feature
can speed up the setting processes.
Audible Signals
The audible signals are beneficial when you are familiarizing yourself with the operating procedure of
the peqPETTE eType electronic pipette. They can also be switched off if desired (see Section 6.3.)
Audible Sound Operation
Low-tone beep Aspirating
High-tone beep Dispensing/ Error codes
2 beeps MD/MIX/SE mode cycle or RESET
routine is completed
Fi
g
ure 10: LCD dis
p
la
y
p
anel.
SYS System Setting
AUTO Auto Pipetting
MD Multiple Dispensing
MIX Mixing
SE Sequential Aspirating/Dispensing
Direction
S
p
eed
Batter
y
Ca
p
acit
y
“RESET“ Button
“Back“ Button
Count No./Pro
g
No.
V
olume (
μ
l)
A
udible Si
g
nals
Blow Out
Reverse Pi
p
ettin
g
“Enter“ Button
“U
p
/Down“ Buttons

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5FUNCTION MAP
The main menu of the peqPETTE eType electronic pipette includes five function modes (SYS, AUTO, MD,
MIX and SE). Each mode includes various function settings (see Figure 11).
Function
Mode
SYS AUTO MD MIX SE
Aspirating
Speed
Dispensing
Speed
Audible
Signals
Blow-out
Reverse
Pipetting
Program
Recall
Basic
Aspirating/
Dispensing
MIX
Mix Number
(COUNT)
Mix Volume
Dispensing
Volume
Dispensing
Number
(COUNT)
Mix Number
(COUNT)
Mix Volume
SA SD
Aspirating
Number
(1, 2, 3,…)
Aspirating
Volume
Dispensing
Number
(1, 2, 3,…)
Dispensing
Volume
Function Map
Function
Settings
Function
Mode
SYS AUTO MD MIX SE
Aspirating
Speed
Dispensing
Speed
Audible
Signals
Blow-out
Reverse
Pipetting
Program
Recall
Basic
Aspirating/
Dispensing
MIX
Mix Number
(COUNT)
Mix Volume
Dispensing
Volume
Dispensing
Number
(COUNT)
Mix Number
(COUNT)
Mix Volume
SA SD
Aspirating
Number
(1, 2, 3,…)
Aspirating
Volume
Dispensing
Number
(1, 2, 3,…)
Dispensing
Volume
Function Map
Function
Settings
6SYS (SYSTEM) MODE
SYS mode is used to set up the function settings before pipetting. Once users select and store these
settings, they will no longer need to set up the settings before every pipetting. There are 6 function
settings (Aspirating Speed, Dispensing Speed, Audible Signals, Blow-out, Reverse Pipetting and
Program Recall) in SYS mode.
Note:
1.
These 6 function settings are sequential procedures. To store these settings in memory, users have to
complete the whole setting procedure and press «ENTER» when the LCD displays .
2.
Users can use the «Up/Down» button to move back and forth to the previous function settings.
6.1 Aspirating Speed
There are 5 speeds available for both aspirating and dispensing:
1. Press «BACK» to enter the function mode selection. Then press «Up/Down» to select the
symbol (flashing).
2. Press «ENTER» to confirm the selection of mode and the aspirating speed symbol will
start flashing.
3. Press «Up/Down» to change the aspirating speed ( : Slowest, : Fastest).
4. Press «ENTER» to confirm the speed selection and move to the “Dispensing Speed” function
setting.
Fi
g
ure 11: Function ma
p
.

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6.2 Dispensing Speed
1. The “Dispensing Speed” symbol should now be flashing.
2. Press «Up/Down» to change the dispensing speed ( : Slowest, : Fastest).
3. Press «ENTER» to confirm the speed selection and move to the “Audible Signals” function
setting.
6.3 Audible Signals
Please refer to Section 4 for the definition of “Audible Signals”.
1. The “Audible Signals” symbol should now be flashing.
2. Press «Up/Down» to select the preference ( : On, : Off).
3. Press «ENTER» to confirm the selection and move to the “Blow-out” function setting.
7BLOW-OUT
The automatic blow-out function, in different pipetting modes, simulates the blow-out function in manual
pipettes. To avoid the leftover final droplet in tips, the blow-out setting is recommended in all protocols.
The blow-out setting will not be performed in “Reverse Pipetting” setting.
1. The “Blow-out” symbol should now be flashing.
2. Press «Up/Down» to select the preference ( : On, : Off).
3. If is selected, pressing «ENTER» will confirm the selection and move to the “PROG”
setting. If is selected, pressing «ENTER» will confirm the selection and move to the
“Reverse Pipetting” setting.
Note:
It is important to withdraw the tip quickly from the dispensed sample vessel after dispensing if Blow-out is selected,
because the piston will automatically retract a short distance. By holding the PLUNGER button during dispensing,
the piston will travel the distance to blow out, and remain there until the PLUNGER button is released.
7.1 Reverse Pipetting
The automatic reverse pipetting, in different pipetting modes, is to reserve the final drop of sample. The
peqPETTE eType electronic pipette will not perform reverse pipetting in MIX or SA modes.
1. The “Reverse Pipetting” symbol should now be flashing.
2. Press «Up/Down» to choose the preference ( : On, : Off).
3. Press «ENTER» to confirm the selection and move to the “PROG” setting.
Note:
If Reverse Pipetting is selected, users have to press the PLUNGER button one more time after pipetting to dispense
the remaining liquid during the pipetting. will appear on the LCD to remind users to dispense the remaining
liquid.

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7.2 Setting PROG (Programs)
The memory program includes 9 different storage locations (PROG 01 ~ 09). Your favorite operating
modes with user-selected settings can be stored to these locations for future recalls. Before setting the
programs, you must have programmed operating mode (e.g. AUTO, MD mode, etc).
Store Operating Mode to Storage Locations
1. Complete the setup of function mode (e.g. AUTO, MD, MIX mode).
2. Press and hold «Down» for 3 seconds to enter PROG mode.
3. Press «Up/Down» to select number from 01 ~ 09.
4. Press «ENTER» to confirm the location selection and save the functions.
Recall Stored Programs from the Storage Location
1. Press «BACK» to enter the function mode selection. Then press «Up/Down» until the
symbol starts flashing.
2. Press «ENTER» repeatedly to confirm the selection of all 6 function settings until the
symbol starts flashing.
3. Press «Up/Down» to select the number.
4. Press «ENTER» to confirm the selection.
5. Press the PLUNGER button to operate the peqPETTE eType electronic pipette in the selected
program.
Note:
During the recall process, the changes of other settings will not be stored.
8AUTO MODE
AUTO mode includes; 1) AUTO mode only and 2) AUTO + MIX mode. In AUTO mode only, the
peqPETTE eType electronic pipette performs the aspirating and dispensing of a set liquid volume. In
AUTO + MIX mode, the peqPETTE eType electronic pipette performs mixing after automatic pipetting is
completed. This mode is ideal for serial dilution applications.
8.1 AUTO Mode Only
Please follow the steps below to set up the automatic pipette volume.
1. Press «BACK» to enter the mode selection, which causes the symbol to start flashing. If
not, press «Up/Down» to select AUTO mode.
2. Press «ENTER» to confirm the mode selection.
3. Use «Up/Down» to select the desired pipetting volume.
4. Press «ENTER» to confirm selected volume and the peqPETTE eType electronic pipette is ready
for pipetting.
Tip:
1.
Users can use the PLUNGER button instead of «Up» to speed up the volume setting.
2.
To have fast volume changes during pipetting works, users can press «ENTER» to make selected
pipetting volume flash. Use «Up/Down» to select the desired pipetting volume. Press «ENTER»
again to confirm the desired pipetting volume.

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8.2 AUTO + MIX Mode
Please follow the steps below to set up the automatic pipette volume.
1. Press «BACK» to enter the mode selection, which causes the symbol to start flashing. If
not, press «Up/Down» to select AUTO mode.
2. Press and hold «ENTER» for 3 seconds to enter AUTO + MIX mode selection. Both AUTO and
MIX symbols will appear. The pipetting volume will appear and start flashing.
3. Use «Up/Down» to select the desired pipetting volume.
4. Press «ENTER» to confirm selected volume and enter MIX mode setting. The symbol
will start flashing.
5. Press «Up/Down» to select the desired mixing cycle counts.
6. Press «ENTER» to confirm selected mixing cycle counts. The mixing volume will start flashing.
7. Press «Up/Down» to select the desired mixing volume.
8. Press «ENTER» to confirm selected mixing volume.
Press the PLUNGER button to aspirate the sample. Press the PLUNGER button again to dispense the
sample and the LCD will display the mixing cycle counts and volume. Press the PLUNGER to mix and
dispense the sample. In the end of mixing, sample will remain in the tip and LCD will display bL. Press
the PLUNGER to dispense the remaining sample with blow-out function.
9MD (MULTIPLE DISPENSING) MODE
In MD mode, the peqPETTE eType electronic pipette performs repetitive dispensing of a selected volume.
When using Multiple Dispense (MD) mode, it is recommended to select Reverse Pipetting in the System
(SYS) settings for best results. Reverse Pipetting is used in Multiple Dispensing (MD) mode to provide
better accuracy and precision with repeated dispensing. Reverse Pipetting means that an extra volume
of liquid will be left in the tip at the end of a multiple dispensing cycle, and the display will show bL to
remind the user to dispense this residual volume before repeating the multiple dispense cycle.
1. Press «BACK» to enter the mode selection.
2. Press «Up/Down» to make the symbol flash.
3. Press «ENTER» to confirm the selection. The default pipetting volume symbol will then appear.
4. Use «Up/Down» to select the desired pipetting volume.
5. Press «ENTER» to confirm the selection of volume. The symbol will start flashing.
6. Use «Up/Down» to select the desired pipetting count.
7. Press «ENTER» to confirm the pipetting count. The pipetting volume will show the total
aspirating volume.
After the settings are completed, first press the PLUNGER button to aspirate the sample according to the
pipetting volume. Then press the PLUNGER button repeatedly to dispense the samples according to the
pipetting counts. For the last count, you will hear two beeps to indicate that the next dispense is the last
and the display will show to remind the user to press the PLUNGER button and dispense the extra
remaining amount of liquid. After completing the Multiple Dispensing cycle, pressing the PLUNGER
button again will aspirate liquid into the tip and repeat the set MD cycle.

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10 MIX MODE
After the settings are completed (see instructions below), Mixing is performed automatically by pressing
the PLUNGER button once.
1. Press «BACK» to enter mode selection.
2. Press «Up/Down» repeatedly until the symbol starts flashing.
3. Press «ENTER» to confirm the selection. The symbol will then appear.
4. Use «Up/Down» to select the desired mixing count.
5. Press «ENTER» to confirm the selection. The default pipetting volume will start flashing.
6. Use «Up/Down» to select the desired mixing volume.
7. Press «ENTER» to confirm the selection. The total mixing volume and counts will be displayed.
Press the PLUNGER button to start the mixing function. After the completion of mixing, pressing the
PLUNGER button will re-activate the mixing process until the user changes the function mode.
11 SE (SEQUENTIAL ASPIRATING/DISPENSING) MODE
SE mode includes SA (Sequential Aspirating) mode and SD (Sequential Dispensing) mode.
11.1 SA (Sequential Aspirating) Mode
The peqPETTE eType electronic pipette performs repetitive aspirations of the selected volumes. An air
gap will be created automatically between two aspirations. When the sequential aspiration is
completed, the next operation will dispense all of the aspiration volume together.
1. Press «BACK» to enter function mode selection.
2. Press «Up/Down» repeatedly until the symbol starts flashing.
3. Press «ENTER» to confirm the selection.
4. Use «Up/Down» until the symbol starts flashing.
5. Press «ENTER» to confirm the selection.
6. Under the symbol, “01” will appear. Press «Up/Down» to set the volume of the first
aspiration.
7. Press «ENTER» to confirm the selection.
8. Repeat steps 6 and 7 to set the sequential aspiration volumes. The total aspirating volume
(including air gap volume) cannot exceed maximum volume range.
9. Press «ENTER» to confirm the selection.
10. The count “01” and aspirating volume will appear on the display. The pipetting direction and
speed will flash to indicate that the peqPETTE eType electronic pipette is ready for liquid pick-
up. Press the PLUNGER button to aspirate the samples. will appear to remind users to create
an air gap (by pressing the PLUNGER button with the tip out of the sample) between sample
aspirations.
11. When the aspirations are completed, the peqPETTE eType electronic pipette will make two
beeping sounds and will flash to indicate the peqPETTE eType electronic pipette is ready for
dispensing.
12. After the sequential aspirating cycle has been completed and the liquid has been dispensed,
pressing the PLUNGER button will re-activate the whole cycle again until users change the
function mode.

Instruction Manual peqPETTE eType
PEQLAB_v0314_E 15
Note:
1.
If the blow-out setting is selected, the peqPETTE eType electronic pipette will automatically blow-out the
liquid during sequential aspiration mode.
2.
Sequential Aspirating (SA) function will not perform Reverse Pipetting even if it is selected in System (SYS)
mode.
11.2 SD (Sequential Dispensing) Mode
The peqPETTE eType electronic pipette performs repetitive dispensing of various selected volumes. As
with Multiple Dispensing mode, for best accuracy in SD Mode, it is recommended to select Reverse
Pipetting in System Mode.
1. Press «BACK» to enter function mode selection.
2. Press «Up/Down» repeatedly until symbol starts flashing.
3. Press «ENTER» to confirm the selection.
4. Press «Up/Down» repeatedly until symbol starts flashing.
5. Press «ENTER» to confirm the selection.
6. Under symbol, “01” will appear. Press «Up/Down» to set the volume of the first
dispense.
7. Press «ENTER» to confirm the selection.
8. Repeat steps 6 and 7 to set the sequential dispensing volumes. The total dispensing volume
cannot exceed the maximum volume range.
9. Press «ENTER» to confirm the selection.
10. The count “01” and the total aspirating volume will appear on the display. The pipetting
direction and speed will flash to indicate the peqPETTE eType electronic pipette is ready for
liquid pick-up. Press the PLUNGER button to aspirate the sample. When the aspiration is
completed, the dispensing count and volume will appear and will flash to indicate that the
peqPETTE eType electronic pipette is ready for dispensing.
11. The peqPETTE eType electronic pipette will make two beeping sounds to remind the user of the
last measured volume to dispense. At the end of the sequential dispensing cycle, if Reverse
Pipette mode is selected, will be displayed to remind the user to press PLUNGER button to
dispense the remaining extra amount of sample. After completing the dispensing, pressing the
PLUNGER button again will re-activate the whole cycle until the user changes the function mode.
Note:
If the blow-out setting is selected, the peqPETTE eType electronic pipette will automatically blow-out the liquid at the
end of a sequential dispensing cycle.

Instruction Manual peqPETTE eType
PEQLAB_v0314_E 16
12 STERILIZATION
Only the lower piston assembly of peqPETTE eType electronic pipette can be steam-autoclaved (121°C,
1 bar, 20 minutes). NOTE: It is important not to exceed the temperature of 121°C or 20 minute time
during the autoclaving cycle. Autoclaving at a higher temperature or for longer period of time will
damage the manifold and void the warranty. The autoclaved parts must be allowed to dry completely at
room temperature for at least 2 hours before reassembly. Users can follow Figures 12 – 15 to
disassemble the peqPETTE eType electronic pipette. Please “DO NOT” use excessive force to pull down
the lower part before releasing the cylinder lock as shown in Figure 12. This action will break the
connection mechanism of the lower part, permanently damaging the instrument and will void the
warranty.
12.1 Removal of the Lower Piston Assembly
Always remember to press RESET button before you disassemble the lower part of peqPETTE eType
electronic pipette.
1. Press the RESET button. Wait until you hear two beeps.
2. Loosen the lower part of peqPETTE eType electronic pipette by unscrewing the connecting nut in
counter-clockwise direction as shown in Figure 13.
3. Pull the lower piston assembly downward slowly until the metal cylinder lock appears as shown
in Figure 14.
4. Lay the peqPETTE eType electronic pipette down on a flat surface. Push the metal cylinder lock
downward until you hear a click sound as shown in Figure 15.
5. The lower piston assembly will come off easily and automatically as shown in Figure 16.
Figure 13: Loosen the connecting
nut by unscrewing counter-
clockwise.
Figure 14: Pull the lower piston
assembly downward slowly until
the cylinder lock appears.
Fi
g
ure 12: Incorrect removal of the lower
p
iston assembl
y
.