VWR OX 4000 H User manual

ba75853e04 06/2012
D.O. meter
OX 4000 H
OPERATING MANUAL
ECN: 664-0038
6
7
1
92
7
O
°
C
mg/l
Auto
TP
Version: 4
Issued: 5, 9, 2012

OX 4000 H
2ba75853e04 06/2012
VWR International bvba
Researchpark Haasrode 2020
Geldenaaksebaan 464
B-3001 Leuven
[phone]
http://be.vwr.com
Printed in Germany.

OX 4000 H Contents
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1 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.1 Authorized use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2 General safety instructions . . . . . . . . . . . . . . . . . . . . . . . . 5
2 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.1 Keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.2 Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.3 Socket field . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.1 General data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.2 Measuring ranges, resolution, accuracy . . . . . . . . . . . . . 13
4 Commissioning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.1 Scope of delivery. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.2 Initial commissioning . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.2.1 Inserting the batteries. . . . . . . . . . . . . . . . . . . . . 15
4.2.2 Switching on the meter. . . . . . . . . . . . . . . . . . . . 16
5 Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.1 General operating principles . . . . . . . . . . . . . . . . . . . . . . 17
5.1.1 Operating modes . . . . . . . . . . . . . . . . . . . . . . . . 17
5.1.2 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.2 Measuring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
5.2.1 Hold function . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5.2.2 General information . . . . . . . . . . . . . . . . . . . . . . 19
5.2.3 Measuring the D.O. concentration . . . . . . . . . . . 20
5.2.4 Measuring the D.O. saturation . . . . . . . . . . . . . . 22
5.2.5 D.O. calibration . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.2.6 Displaying calibration records . . . . . . . . . . . . . . 24
5.2.7 Entering the salt content (salinity) . . . . . . . . . . . 25
5.3 Storage in memory . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
5.3.1 Storing measurement data. . . . . . . . . . . . . . . . . 26
5.3.2 Downloading the data memory . . . . . . . . . . . . . 28
5.3.3 Erasing the data memory . . . . . . . . . . . . . . . . . . 29
5.3.4 Downloading calibration data . . . . . . . . . . . . . . . 29
5.4 Transmitting data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
5.4.1 Options for data transmission . . . . . . . . . . . . . . 31
5.4.2 Downloading stored measurement datasets . . . 31
5.4.3 Downloading calibration data . . . . . . . . . . . . . . . 31
5.4.4 USB interface (device) . . . . . . . . . . . . . . . . . . . . 32
5.5 Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.5.1 System settings . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.5.2 Measurement settings . . . . . . . . . . . . . . . . . . . . 36
5.6 Reset. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
5.6.1 Resetting the measurement settings. . . . . . . . . 38

Contents OX 4000 H
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5.6.2 Resetting the system settings. . . . . . . . . . . . . . . 39
6 Maintenance, cleaning, disposal,
accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
6.1 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
6.1.1 Replacing the batteries . . . . . . . . . . . . . . . . . . . . 41
6.2 Cleaning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
6.3 Packing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
6.4 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
6.5 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
7 What to do if... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
8 Index. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
9 Technical service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
10 Warranty. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

OX 4000 H Safety
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1 Safety
This operating manual contains basic instructions that you must follow
during the commissioning, operation and maintenance of the meter.
Consequently, all responsible personnel must read this operating man-
ual carefully before working with the meter. The operating manual must
always be available within the vicinity of the meter.
Target group The meter was developed for work in the field and in the laboratory.
Thus, we assume that, as a result of their professional training and
experience, the operators will know the necessary safety precautions
to take when handling chemicals.
Safety instructions Safety instructions in this operating manual are indicated by the warn-
ing symbol (triangle) in the left column. The signal word (e.g. "Caution")
indicates the level of danger:
Warning
indicates instructions that must be followed precisely in order to
avoid possibly great dangers to personnel.
Caution
indicates instructions that must be followed precisely in order to
avoid the possibility of slight injuries or damage to the meter or
the environment.
Further notes
Note
indicates notes that draw your attention to special features.
Note
indicates cross-references to other documents, e.g. operating manu-
als.
1.1 Authorized use
This meter is authorized exclusively for oxygen measurements in a field
and laboratory environment.
The technical specifications as given in chapter 3 TECHNICAL DATA must
be observed. Only the operation and running of the meter according to
the instructions given in this operating manual is authorized.
Any other use is considered unauthorized.
1.2 General safety instructions
This meter is constructed and tested in compliance with the IEC 1010
safety regulations for electronic measuring instruments.
It left the factory in a safe and secure technical condition.

Safety OX 4000 H
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Function and
operational safety
The smooth functioning and operational safety of the meter can only be
guaranteed if the generally applicable safety measures and the specific
safety instructions in this operating manual are followed during opera-
tion.
The smooth functioning and operational safety of the meter can only be
guaranteed under the environmental conditions that are specified in
chapter 3 TECHNICAL DATA.
If the meter was transported from a cold environment to a warm envi-
ronment, the formation of condensate can lead to the faulty functioning
of the meter. In this event, wait until the temperature of the meter
reaches room temperature before putting the meter back into opera-
tion.
Caution
The meter is only allowed to be opened by authorized personnel.
Safe operation If safe operation is no longer possible, the meter must be taken out of
service and secured against inadvertent operation!
Safe operation is no longer possible if the meter:
has been damaged in transport
has been stored under adverse conditions for a lengthy period of
time
is visibly damaged
no longer operates as described in this manual.
If you are in any doubt, please contact the supplier of the meter.
Obligations of the
purchaser
The purchaser of this meter must ensure that the following laws and
guidelines are observed when using dangerous substances:
EEC directives for protective labor legislation
National protective labor legislation
Safety regulations
Safety datasheets of the chemical manufacturers.
Caution
In addition to the safety instructions mentioned here, also follow
the safety instructions of the sensors used.
The operating manuals of the sensors are available on the
supplied CD.

OX 4000 H Overview
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2Overview
The OX 4000 H compact precision dissolved oxygen (D.O.) meter
enables you to perform D.O. measurements quickly and reliably.
The OX 4000 H provides the maximum degree of reliability, ease of
use and measuring certainty for all applications.
1Keypad
2Display
3Socket field
1
2
3
6
7
1
92
7
O
°
C
mg/l
Auto
TP

Overview OX 4000 H
8ba75853e04 06/2012
2.1 Keypad
In this operating manual, keys are indicated by brackets <..> .
The key symbol (e.g. <OK>) generally indicates a short keystroke
(under 2 sec) in this operating manual. A long keystroke (approx.
2 sec) is indicated by the underscore behind the key symbol (e.g.
<OK_>).
<ON/OFF>:
<ON/OFF_>:
Switches the meter on or off
Resets calibration data
<MODE>:
<MODE_>:
Selects the measured parameter
Opens the setting menu for calibration and mea-
surements
<CAL>:
<CAL_>:
Calls up the calibration procedure
Displays the calibration data
<STR>: Saves a measured value manually
<RCL>: Displays the manually stored measured values
<>
<> :
Increments values
Decrements values
<OK>:
<OK_>:
Confirms entries
Opens the setting menu for system settings
<HOLD>: Freezes the measured value (HOLD function)

OX 4000 H Overview
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2.2 Display
Function display
indicators
1Status information
2Measured value (with unit)
3Measured parameter
4Calibration evaluation
5Measured temperature (with unit)
O
C
%
mg/l
°
mbar
Sal
°
F
3
4
5
1
2
8
88
8
18
8
8
LoBat
Auto Store
TP
Time
Day.Month
Year
Baud
No.
Ident
RCL
S
Cal
LoBat With battery operation: batteries almost empty
TP Temperature measurement active
Store Saving
Auto Automatic stability control
S The current measured value is retained (HOLD)
RCL The data storage is open (RCL)

Overview OX 4000 H
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2.3 Socket field
1D.O. sensor
2USB-B interface (device)
3Service interface
312

OX 4000 H Technical data
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3 Technical data
3.1 General data
Dimensions Approx. 190 x 85 x 50 mm
Weight Approx. 0,4 kg
Mechanical structure Type of protection IP 67
Electrical safety Protective class III
Test certificates CE
Ambient
conditions
Storage - 25 °C ... + 65 °C
Operation 0 °C ... + 55 °C
Allowable relative hu-
midity
Annual mean: < 75 %
30 days/year: 95 %
Other days: 85 %
Power
supply
Batteries 4 x 1.5 V alkali-manganese batteries, type
AA
Rechargeable batter-
ies
4 x 1.2 V NiMH rechargeable batteries,
type AA
(no charging function)
Operational life up to 1000 h (operating hours with batteries)
Service
interface
This interface can be used for service purposes only.
USB interface Type USB 1.1 (device)
USB-B (device), data output
Baud rate Can be set to
1200, 2400, 4800, 9600 Baud
Data bits 8
Stop bits 2
Parity None
Handshake RTS/CTS
Cable length max. 3 m

Technical data OX 4000 H
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Guidelines
and norms used
EMC EC guideline 89/336/EEC
EN 61326
EN 61000-3-2
EN 61000-3-3
FCC Class A
Meter safety EC guideline 73/23/EEC
EN 61010-1
ANSI/UL 61010-1
CAN/CSA-C22.2 No. 61010-1
IP protection class EN 60529
FCC Class A Equipment Statement
Note: This equipment has been tested and found to comply with
the limits for a Class A digital device, pursuant to Part 15 of the
FCC Rules. These limits are designed to provide reasonable
protection against harmful interference when the equipment is
operated in a commercial environment. This equipment
generates, uses, and can radiate radio frequency energy and, if
not installed and used in accordance with the instruction manual,
may cause harmful interference to radio communications.
Operation of this equipment in a residential area is likely to cause
harmful interference in which case the user will be required to
correct the interference at his own expense.

OX 4000 H Technical data
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3.2 Measuring ranges, resolution, accuracy
Note
The measuring ranges and accuracy values specified here apply exclu-
sively to the meter. The accuracy of the sensors has also to be taken
into account.
Measuring ranges,
resolution
Parameter Measuring range Resolution
D.O. concentration
[mg/l]
0 ... 20.00 0.01
Saturation [%] 0 ... 200.0 0.1
T [°C] 0 ... 50.0 0.1
Accuracy (± 1 digit) Parameter Accuracy
D.O. concentration
[mg/l]
± 0.5 % of measured value
at ambient temperature + 5 °C ... + 30 °C
Saturation [%] ± 0.5% of measured value
when measuring in the range of ± 10 K
around the calibration temperature
T (°C) ± 0.1
Correction functions Temperature
compensation
Accuracy better than 2 % at 0 ... + 40 °C
Salinity correction 0.00 ... 35.0 SAL
Air pressure correc-
tion
Automatic through integrated
pressure sensor in the range of 500 ... 1100
mbar

Technical data OX 4000 H
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OX 4000 H Commissioning
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4 Commissioning
4.1 Scope of delivery
Meter OX 4000 H
4 batteries 1.5 V Mignon type AA
Short instructions
CD-ROM with detailed operating manual
4.2 Initial commissioning
Perform the following activities:
Insert the supplied batteries
Switching on the meter
4.2.1 Inserting the batteries
Caution
Make sure that the poles of the batteries are positioned correctly.
The ±signs on the batteries must correspond to the ± signs in the
1Unscrew the four screws (1) on the underside of the meter.
2 Open the battery compartment (2) on the underside of the
meter.
3Place four batteries (type Mignon AA) in the battery compart-
ment.
4 Close the battery compartment (2) and tighten the screws (1).
1
2

Commissioning OX 4000 H
16 ba75853e04 06/2012
battery compartment.
Note
Alternatively, you can also use Ni-MH rechargeable batteries (type
Mignon AA). In order to charge the batteries, an external charging
device is required.
4.2.2 Switching on the meter
Switch-off interval The meter has an energy saving feature to avoid unnecessary battery
depletion during battery operation.
The energy saving feature switches off the meter if no key was pressed
during the specified interval (setting the switch-off interval see section
5.5.1).
Display illumination During battery operation, the meter automatically switches off the dis-
play illumination if no key has been pressed for 30 seconds (setting the
display illumination see section 5.5.1). The illumination is switched on
with the next keystroke again.
1Press the <ON/OFF> key.
A display test is briefly displayed.
Subsequently, the meter switches to the measuring mode
(measured value display).

OX 4000 H Operation
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5 Operation
5.1 General operating principles
This section contains basic information on the operation of the
OX 4000 H.
5.1.1 Operating modes
The meter has the following operating modes:
Measurement
The display indicates the measurement data in the measured value
display
Calibration
The display guides you through a calibration procedure with calibra-
tion information
Storage
The meter stores measurement data
Configuration
Settings and functions are displayed
5.1.2 Operation
Keys The meter is operated via keys. The keys can have different functions
with long or short keystrokes.
Functions Generally, a function is carried out with a short keystroke. A long key-
stroke opens a setting menu.
In a setting menu, settings are selected with the <><>keys.
A setting is confirmed with <OK>. With confirming, the setting is fin-
ished and the next setting is displayed.
Representation In this operating manual, keys are indicated by brackets <..> .
The key symbol (e.g. <OK>) generally indicates a short keystroke
(under 2 sec) in this operating manual. A long keystroke (approx.
2 sec) is indicated by the underscore behind the key symbol (e.g.
<OK_>).

Operation OX 4000 H
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5.2 Measuring
Preparatory activities Perform the following preparatory activities when you want to measure:
Stability control
[Auto]
During the measuring procedure, the stability control function is auto-
matically activated. The stability control function [Auto] checks the sta-
bility of the measured oxygen signal and the stability of the measured
temperature signal. The stability has a considerable effect on the repro-
ducibility of the measured value.
The status display [Auto] flashes until a stable measured value is avail-
able. As soon as the measured value is stable the status display [Auto]
stops flashing.
For identical measurement conditions, the following applies:
Temperature sensor The temperature measurement is absolutely essential for a reproduc-
ible oxygen measurement. If a temperature sensor is integrated in the
sensor, it is indicated on the display by [TP].
1Connect the D.O. sensor to the meter.
2 Calibrate or check the meter with the sensor.
3Select the measured parameter with <MODE>.
Measured parameter Time interval Stability in the time
interval
D.O. concentration 10 seconds Δ: better than 0.05 mg/l
DO saturation 10 seconds Δ: better than 0.6 %
Temperature 10 seconds Δ: better than 0.02 °C

OX 4000 H Operation
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5.2.1 Hold function
5.2.2 General information
You can measure the following parameters:
D.O. concentration
DO saturation
Preparatory activities Perform the following preparatory activities when you want to measure:
Note
Incorrect calibration of D.O. sensors will result in incorrect measured
values. Calibrate at regular intervals.
1Freeze the current measured value with <HOLD> (Hold func-
tion).
An S is displayed in front of the current measured value as long
as the Hold function is active.
2To terminate the Hold function: press the <MODE> or <HOLD>
key. The S in front of the measured value is no longer dis-
played.
S
0
5
2
C
°
TP
mg/l
O
Auto
3
6
7
1Connect the D.O. sensor to the meter.
2 Calibrate or check the meter with the D.O. sensor. How to cal-
ibrate is described in section 5.2.5.
3Select the measuring mode with <MODE>.

Operation OX 4000 H
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Note
The OX 4000 H D.O. meter automatically recognizes the type of con-
nected D.O. sensor (OXY 11).
Temperature sensor The D.O. sensor has an integrated temperature sensor that always
measures the current temperature of the test sample.
Warning
When connecting an earthed PC/printer, measurements cannot be per-
formed in earthed media as incorrect values would result. The RS232
interface is not galvanically isolated.
5.2.3 Measuring the D.O. concentration
Note
When measuring the concentration of test samples with a salt content
of more than 1 g/l, a salinity correction is required. For this, you have to
measure and input the salinity of the test sample first. This is described
in section 5.2.7 ENTERING THE SALT CONTENT (SALINITY).
Switch on the salinity correction prior to measuring the D.O. content
(see below).
You can measure the D.O. content without salinity correction as fol-
lows:
Stability control The stability control function continuously checks the stability of the
measurement signal. The stability has a considerable impact on the
reproducibility of measured values. The [Auto] display flashes until a
stable measured value is available.
1Perform the preparatory activities according to section 5.2.2.
2 Immerse the D. O. sensor in the test sample.
3Press the <MODE><> key repeatedly until the D. O. concen-
tration in mg/l appears on the display.
0
5
2
C
°
TP
mg/l
O
Auto
3
6
7