VWR AVANTOR PV4 User manual

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Version 01 Issue 02/2022
Instruction manual
VWR®PV4 Spectrophotometer
NA Cat. No 76520-714

Legal address of Manufacturer
United States
VWR International LLC
100 Matsonford Rd
Radnor, PA 19087
[phone]
www.vwr.com
Country of Origin
China

Table of Contents
Spectrophotometer..............................................................................................................................................1
Safety Information.......................................................................................................................................1
Package Contents.......................................................................................................................................2
Unpacking..................................................................................................................................................... 2
Installation.................................................................................................................................................... 2
Intended use................................................................................................................................................. 2
Symbols and conventions......................................................................................................................... 3
Product Specifications............................................................................................................................... 3
Overview....................................................................................................................................................... 4
Description....................................................................................................................................................5
Getting Started............................................................................................................................................ 6
Important Guidelines..................................................................................................................................6
General Operating Instructions............................................................................................................... 6
Calibration and System Settings............................................................................................................12
Performance Verification......................................................................................................................... 17
Operation................................................................................................................................................... 20
Troubleshooting......................................................................................................................................... 31
Repair and maintenance......................................................................................................................... 32
User replaceable accessories and spare parts................................................................................ 346
Technical service.....................................................................................................................................367
Warranty..................................................................................................................................................... 37
Compliance with local laws and regulations......................................................................................38
Instrument disposal.................................................................................................................................. 38
Software.............................................................................................................................................................. 39
Functions.....................................................................................................................................................39
Installation............................................................................................................................................... 400
Introduction..............................................................................................................................................455
General Operating Instructions............................................................................................................477
Operation................................................................................................................................................... 50
Appendix.................................................................................................................................................... 60

1vwr.com I Instruction manual VWR®PV4 Spectrophotometer
Spectrophotometer
Safety Information
Avoiding Electrical Shock
Please follow the guidelines below and read this manual in its entirety to ensure safe operation of
the unit.
VWR recommends against the use of PV4 Spectrophotometer.
To avoid electrical shock
▪Do not open the device.
▪Disconnect the device from the main supply before carrying out
maintenance work or changing the fuses.
▪The inside of the device is a high-voltage area Danger!
▪Do not use the device if it is damaged, especially if the main
power cable is in any way damaged or defective.
▪Repairs may only be carried out by the service technicians from
your local VWR office and authorized contractual partners.
▪The device must be connected to a power outlet that has a
protective ground connection.
▪If the instrument is used in a manner not specified by the
manufacturer, the protection provided by the instrument may
be impaired.
Avoiding Damage to the Instrument
▪Do not allow any liquid to enter into the device.
▪Do not operate the device in a hazardous location or potentially
explosive environment.

2vwr.com I Instruction manual VWR®PV4 Spectrophotometer
Package Contents
Description
Quantity
Spectrophotometer
1PC
Glass Cuvette
4PCS
Power Cord
1PC
Software USB Drive
1PC
USB Cable
1PC
Quick Manual
1PC
Dust Cover
1PC
Fuse
1PC
Unpacking
Open the package, according to carefully check the packing list for all items. If items are missing
or damaged, please contact your local VWR/Avantor service provider or authorized contractual
partner.
Installation
Placement
Place the instrument on a stable surface carefully.
Install printer (Optional)
Check to confirm instrument power switch is turned off before connecting the printer data cable
to the Instrument’s serial/USB port.
Connect the power cord
First, confirm that the instrument power switch is turned off, then plug the power cord into the
power interface and the power supply socket apparatus.

3vwr.com I Instruction manual VWR®PV4 Spectrophotometer
Intended use
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION This symbol indicates a potential risk and alerts you to
proceed with caution
CAUTION This symbol indicates the presence of high voltage and
warns the user to proceed with caution
CAUTION This symbol indicates risk associated with hot surfaces
Product Specifications
Optical System
Single Beam
Wavelength Range
320-1100nm
Bandwidth
2nm
Stray Light
≤0.05%T @ 360nm
Photometric Range
0 to 200%T, -0.3 to 3.0A, 0 to 9999C
Wavelength Accuracy
±0.5nm
Photometric Accuracy
±0.4%T or ±0.004A @ 1A
Stability
0.002A/h @ 500nm
Memory
236KB
Language
English, French, German, Spanish, Portuguese, Chinese
Display
TFT Color LCD (5”, Touch Screen)

4vwr.com I Instruction manual VWR®PV4 Spectrophotometer
Interface
USB-A, USB-B,RS-232
Measuring Procedure
Photometry, Quantitation, Spectrum Scan
Power Supply
AC 100~240 V, 50~60 Hz
Dimension
456×360×185
Weight
10.5kg
Work Environment
15 to 35℃, 15 to 70% relative humidity
Store Environment
-10 to 50℃, 15 to 70% relative humidity
This instrument is compliant to the U.S. and Canada Directives on
UL 61010-1 Issued: 2012/05/11 Ed: 3
CSA-C22.2 No. 61010-1 Issued: 2012/05/11 Ed: 3
47CFR Part 15 (2011)
ANSI C63.4 (2009)
Overview
The PV4 Spectrophotometer is used in Chemistry, Pharmaceuticals, Biochemical, Metallurgy,
Light Industry, Textile, Material, Environments, Medical, Education and some other fields for
Quality Control laboratories.

5vwr.com I Instruction manual VWR®PV4 Spectrophotometer
Description
Front View
Right View
Rear View

6vwr.com I Instruction manual VWR®PV4 Spectrophotometer
Getting Started
Turn On and Self-check
Switch on the power. Self-check includes the following steps: Turn On Lamp → Locating Filter Disc
→ Locating Automatic Sample Holder (If Installed) → Get Dark Current → Locating Wavelength →
Check Energy → Check System baseline.
Important Guidelines
▪Reagents and dilution buffers can cause cauterization and other damage to health.
▪Samples (nucleic acids, proteins, bacteria cultures) can be infectious and cause serious
damage to health.
▪During sample preparation, measuring procedures, maintenance and cleaning work,
observe all local laboratory safety precautions (e.g. wear protective clothing and gloves,
use of disinfectant) regarding the handling of sample material.
▪Dispose of measuring solutions, cleaning and disinfectant materials in accordance with the
relevant local laboratory regulations.