VWR AVANTOR PV4 User manual

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Version 01 Issue 02/2022
Instruction manual
VWR®PV4 Spectrophotometer
NA Cat. No 76520-714

Legal address of Manufacturer
United States
VWR International LLC
100 Matsonford Rd
Radnor, PA 19087
[phone]
www.vwr.com
Country of Origin
China

Table of Contents
Spectrophotometer..............................................................................................................................................1
Safety Information.......................................................................................................................................1
Package Contents.......................................................................................................................................2
Unpacking..................................................................................................................................................... 2
Installation.................................................................................................................................................... 2
Intended use................................................................................................................................................. 2
Symbols and conventions......................................................................................................................... 3
Product Specifications............................................................................................................................... 3
Overview....................................................................................................................................................... 4
Description....................................................................................................................................................5
Getting Started............................................................................................................................................ 6
Important Guidelines..................................................................................................................................6
General Operating Instructions............................................................................................................... 6
Calibration and System Settings............................................................................................................12
Performance Verification......................................................................................................................... 17
Operation................................................................................................................................................... 20
Troubleshooting......................................................................................................................................... 31
Repair and maintenance......................................................................................................................... 32
User replaceable accessories and spare parts................................................................................ 346
Technical service.....................................................................................................................................367
Warranty..................................................................................................................................................... 37
Compliance with local laws and regulations......................................................................................38
Instrument disposal.................................................................................................................................. 38
Software.............................................................................................................................................................. 39
Functions.....................................................................................................................................................39
Installation............................................................................................................................................... 400
Introduction..............................................................................................................................................455
General Operating Instructions............................................................................................................477
Operation................................................................................................................................................... 50
Appendix.................................................................................................................................................... 60

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Spectrophotometer
Safety Information
Avoiding Electrical Shock
Please follow the guidelines below and read this manual in its entirety to ensure safe operation of
the unit.
VWR recommends against the use of PV4 Spectrophotometer.
To avoid electrical shock
▪Do not open the device.
▪Disconnect the device from the main supply before carrying out
maintenance work or changing the fuses.
▪The inside of the device is a high-voltage area Danger!
▪Do not use the device if it is damaged, especially if the main
power cable is in any way damaged or defective.
▪Repairs may only be carried out by the service technicians from
your local VWR office and authorized contractual partners.
▪The device must be connected to a power outlet that has a
protective ground connection.
▪If the instrument is used in a manner not specified by the
manufacturer, the protection provided by the instrument may
be impaired.
Avoiding Damage to the Instrument
▪Do not allow any liquid to enter into the device.
▪Do not operate the device in a hazardous location or potentially
explosive environment.

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Package Contents
Description
Quantity
Spectrophotometer
1PC
Glass Cuvette
4PCS
Power Cord
1PC
Software USB Drive
1PC
USB Cable
1PC
Quick Manual
1PC
Dust Cover
1PC
Fuse
1PC
Unpacking
Open the package, according to carefully check the packing list for all items. If items are missing
or damaged, please contact your local VWR/Avantor service provider or authorized contractual
partner.
Installation
Placement
Place the instrument on a stable surface carefully.
Install printer (Optional)
Check to confirm instrument power switch is turned off before connecting the printer data cable
to the Instrument’s serial/USB port.
Connect the power cord
First, confirm that the instrument power switch is turned off, then plug the power cord into the
power interface and the power supply socket apparatus.

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Intended use
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION This symbol indicates a potential risk and alerts you to
proceed with caution
CAUTION This symbol indicates the presence of high voltage and
warns the user to proceed with caution
CAUTION This symbol indicates risk associated with hot surfaces
Product Specifications
Optical System
Single Beam
Wavelength Range
320-1100nm
Bandwidth
2nm
Stray Light
≤0.05%T @ 360nm
Photometric Range
0 to 200%T, -0.3 to 3.0A, 0 to 9999C
Wavelength Accuracy
±0.5nm
Photometric Accuracy
±0.4%T or ±0.004A @ 1A
Stability
0.002A/h @ 500nm
Memory
236KB
Language
English, French, German, Spanish, Portuguese, Chinese
Display
TFT Color LCD (5”, Touch Screen)

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Interface
USB-A, USB-B,RS-232
Measuring Procedure
Photometry, Quantitation, Spectrum Scan
Power Supply
AC 100~240 V, 50~60 Hz
Dimension
456×360×185
Weight
10.5kg
Work Environment
15 to 35℃, 15 to 70% relative humidity
Store Environment
-10 to 50℃, 15 to 70% relative humidity
This instrument is compliant to the U.S. and Canada Directives on
UL 61010-1 Issued: 2012/05/11 Ed: 3
CSA-C22.2 No. 61010-1 Issued: 2012/05/11 Ed: 3
47CFR Part 15 (2011)
ANSI C63.4 (2009)
Overview
The PV4 Spectrophotometer is used in Chemistry, Pharmaceuticals, Biochemical, Metallurgy,
Light Industry, Textile, Material, Environments, Medical, Education and some other fields for
Quality Control laboratories.

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Description
Front View
Right View
Rear View

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Getting Started
Turn On and Self-check
Switch on the power. Self-check includes the following steps: Turn On Lamp → Locating Filter Disc
→ Locating Automatic Sample Holder (If Installed) → Get Dark Current → Locating Wavelength →
Check Energy → Check System baseline.
Important Guidelines
▪Reagents and dilution buffers can cause cauterization and other damage to health.
▪Samples (nucleic acids, proteins, bacteria cultures) can be infectious and cause serious
damage to health.
▪During sample preparation, measuring procedures, maintenance and cleaning work,
observe all local laboratory safety precautions (e.g. wear protective clothing and gloves,
use of disinfectant) regarding the handling of sample material.
▪Dispose of measuring solutions, cleaning and disinfectant materials in accordance with the
relevant local laboratory regulations.

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General Operating Instructions
Touch Screen Using Tips
The screen can be started with a touch. To make a selection, use your fingertips or stylus to press
the screen. Do not press the screen with sharp objects (such as ball point).
Select Application
At he Main Interface, press the icon to select application.

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Photometry Measure the absorbance or transmittance of the sample.
Quantitation Establish the standard curve and measure the concentration
of the sample.
Spectrum Scan the sample in a wavelength range.
File Manage files stored in the instrument or USB disk.
System System calibration and setup.
Performance verification Verify the performance of the instrument.
Basic Operation
Home Back to main interface.
Return Back to the previous interface.
/
Page Up/Down Go to previous/next page.

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Measurement Results Operation
Open Open result(s) from internal/USB memory.
Save Save result(s) to internal/USB memory.
Print Print result(s).
Delete Delete selected result(s).
Rename, Print and Delete Results
Rename a Sample:
List interface, press the area Name, key in the
sample name (Up to 8 characters).
Print the Measurement Report:
List interface, press the icon .
Delete sample(s):
List interface, press the Check Box, and press
the icon .
Open Results

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Open:
List interface, press the icon .
Press the icon /to select the
Internal/USB memory which the file saved.
Press file lists to select, press the button Open.
Save Results

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Save:
List interface, press the icon .
Press the icon / to select the
Internal/USB memory which the file to save.
Type in the file name, press the button Save.
Files Operation
Internal Memory Internal memory of the spectrophotometer.
USB Memory USB extended mass memory.
Copy Copy the selected file(s) from internal /USB memory to
USB/internal memory.
Export csv Export file(s) To *.csv format
Export txt Export file(s) To *.txt format
Delete Delete the selected file(s).
Rename, Import, Export and Delete Files

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Rename a File:
File management interface, press the area
Name, key in the file name (Up to 8 characters).
Copy File(s) From/To Internal
Memory/USB Memory:
File management interface, press the Check
Box, press the button (Need USB disk) .
Export File(s) To *.csv Format:
File management interface, press the Check
Box, press the button (Need USB disk) .
Export File(s) To *.txt Format:
File management interface, press the Check
Box, press the button (Need USB disk) .
Delete File(s):
File management interface, press the Check
Box, and press the icon .
Calibration and System Settings
Select the icon in the main interface. Display options to calibrate the system and
configure the basic instrument settings.
Calibration
Calibrate Starts the calibration process.
Select Tab Calibration in the System interface. Remove something in the measurement channel,
close the sample chamber cover, select the item Dark current,Wavelength or System baseline,
press the icon to do calibration.

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Settings of Light Source
Tungsten lamp reset Reset the Tungsten lamp usage.
Select Tab Light source in the System interface. The light source information is displayed on the
screen.
Reset the lamp usage:
Press the icon to reset the Tungsten lamp usage
time.

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Edit Clock
Accept Accepts the new value.
Select Tab Clock in the System interface. Press the value of year, month, date, hour, minute or
second to change. Press the icon to accept new value.
Memory Management
Format Internal Memory Format the internal memory of the
spectrophotometer.
Format USB Memory Format the USB mass storage.
Select Tab Memory in the System interface. The use of the internal and USB memory (If inserted)
are shown. Press the icon / to format internal memory/USB memory.

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Language Selection
Accept Accept the new language.
Select Tab Language in the System interface. Select a language, press the icon to change.
General Options
Select Tab General Options in the System interface.
Beep:
Press the icon to turn on/off the beep.

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Brightness:
Press the icon / to decrease/increase
the brightness of the LCD display.
Close display:
Press the icon to turn on/off. If turned
on, the display will close automatically if no
operation for 30 minutes.
Sample holder:
If you have a new automatic sample holder
installed, you need to set the sample holder type
by pressing the icon
/ (AC-5: 5 cells automatic sample
holder, AC-8：8 cells automatic sample holder).
Restore Defaults
Restore Restore the parameters to factory settings.
Select Tab Restore defaults in the System interface. Select an item, press the icon to
restore.