VWR V-1200 User manual

VWR Collection Manual ver 1, rel 15.05.2007
V-1200 Spectrophotometer
INSTRUCTION MANUAL
European Catalogue Number(s):
634-6000
Version: 1.0.0
Issued: 07, April 2010

Legal Address of Manufacturer
Europe
VWR International Europe bvba
Researchpark Haasrode 2020
Geldenaaksebaan 464
B 3001 Leuven
Tel.:+ 32 16 385 011
http://www.be.vwr.com
Made in China

Table of Contents
Safety Information 1
Package Contents 1
Unpacking 2
Installation 2
Intended Use 2
Symbols and Conventions 2
Specifications 3
Overview 4
Description of Buttons and Switches 4
Operational Keys 5
Getting Started 6
Important Guidelines 6
General Operating Instructions 7
Operation 8
Photometry 9
Quantitative Mode 10
System Utility 15
Troubleshooting 18
Repair and Maintenance 19
Daily Maintain 19
Spare Parts Replacement 19
Accessories and Spares Parts 24
Technical Service 24
Warranty 25
Equipment Disposal 25

1
Safety Information
Please follow the guidelines below, and read this manual in its entirety to ensure safe
operation of the unit.
VWR recommends against the use of V-1200 Spectrophotometer.
Do not open the device.
Disconnect the device from the mains supply before carrying out
maintenance work or changing the fuses.
The inside of the device is a high-voltage area Danger!
Do not use the device if it is damaged, especially if the main power
cable is in any way damaged or defective.
Repairs may only be carried out by the service technicians from your
local VWR office and authorized contractual partners.
The device must be connected to a power outlet that has a protective
ground connection.
If the equipment is used in a manner not specified by the
manufacturer, the protection provided by the equipment may be
impaired.
Do not allow any liquid to enter into the device.
 Do not operate the device in a hazardous location or potentially
explosive environment.
Package Contents
Description Quantity
Spectrophotometer 1PC
Glass Cuvette 4PCS
Power Cord ( Euro Plug ) 1PC
Power Cord ( UK Plug ) 1PC
Power Cord ( SW Plug ) 1PC

2
Manual 1PC
Unpacking
Open the package, according to carefully check the packaging packing list items, if found
inside the packaging are missing or damaged items please your local VWR office and
authorized contractual partners.
Installation
Placement
Place the instrument on the stable table carefully.
Install printer (Printer is Optional Accessories)
Check to confirm instrument power switch is turned off, connect the printer’s data cable to
the Instrument’s parallel port.
Link the power cord
Check to confirm instrument power switch is turned off, the power cord plug into two
separate power interface and power supply socket apparatus.
Intended Use
V-1200 Spectrophotometer used in Chemistry, Pharmaceuticals, Biochemical, metallurgy,
Light Industry, Textile, Material, Environments, Medical, Education and some other fields. It
is one of the most important instruments in Quality Control and an essential in normal
laboratories.

3
Symbols and Conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION This symbol indicates a potential risk and alerts you to
proceed with caution
CAUTION This symbol indicates the presence of high voltage and
warns the user to proceed with caution
CAUTION This symbol indicates risks associated with hot surfaces
Specifications
Optical System Single Beam
Wavelength Range 325-1000nm
Band Width 4nm
Stray Light ≤0.3%T
Photometric Range 0-200%T,-0.3-3.0A, 0-9999C
Wavelength Accuracy ±2nm
Photometric Accuracy ≤±0.5%T or 0.005A@1A
Stability 0.002A/h @ 500nm
Memory 200 Results & 200 Standard Curves
Language English, French, German, Spanish
Display 128×64 Dots Matrix LCD
Interface USB, Parallel
Measuring Procedure: Photometry
Quantitation
Power Supply AC 110/220V, 50/60Hz
Dimension 490×360×210mm
Weight 12kg

4
This instrument is compliant to the European Directives on
Low Voltage Directive 2006/95/EC
Electromagnetic compatibility 2004/108/EC
Restriction on use of Hazardous Substances RoHS 2002/95/EC
and theirs amandements.
Overview
V-1200 Spectrophotometer has the characters of wide range of wavelength, high sensitivity,
powerful function, easy to use, simple structure and pretty figure. Besides these, the large
LCD, High Precise A/D and easy to store RAM makes the instrument much more superior
than other originals. It is widely used in Chemistry, Pharmaceuticals, Biochemical,
metallurgy, Light Industry, Textile, Material, Environments, Medical, Education and some
other fields. It is one of the most important instruments in Quality Control and an essential
in normal laboratories.
Description of Buttons and Switches
Front View
Rear View

5
Operational Keys

6
Key Functions
FUNCTION: Functions on-screen prompts
PRINT: Print measuring result
ZERO: Blank
SET: Set Parameters
GOTO ʎ: Set Wavelength
, UP, DOWN: Scroll menu/data and set Y scale
Getting Started
The following chart describes the basic operation of the instrument.
Turn On and Self-check
Switch on the power. Then the instrument begins to self-check and 15 minutes’ warm up.
Self-check includes the following steps: Turn on Lamp →Check Sensor →System
Position →initialize A/D →Get Dark Current.
Important Guidelines
Reagents and dilution buffers can cause cauterization and other damage to health.
Samples (nucleic acids, proteins, bacteria cultures) can be infectious and cause

7
serious damage to health.
During sample preparation, measuring procedures and maintenance and cleaning
work, observe all local laboratory safety precautions (e.g. wear protective clothing
and gloves, use of disinfectant) regarding the handling of sample material.
Dispose of measuring solutions and cleaning and disinfectant materials in
accordance with the relevant local laboratory regulations.
General Operating Instructions
Select Application
Main interface, press the key (left) to enter into.
Set Wavelength
Test Interface, press key to set wavelength, , to modify
wavelength value, then press key (left) to go to wavelength and blank.
Set Parameters
Press enter into setup interface, , to select items or input
parameters, (left) to confirm.
Delete the test result and stored data
Test Interface, press key , then press key , to select ”Clear Data,
not Print ”, (left) to delete.
Blank

8
Put the Reference in the light path, press to do blank.
Measure Samples
Put the samples in the light path, press (left) to measure.
Print the test results
Test Interface, press key , , select “Print, clear data”, press key
(left) to print.
Store the Standard Curve
After got the Standard Curve by Marked, press , input the file name and
press (left) to save.
Load the Standard Curve
“Quantitative” interface, press , to select “Load Curve”, press ,
to choose the curve you want, press (left) to open.
Operation
Self-check
Remove all the blocks in the light path and close the lid of the compartment; Switch on the
power supply to begin the self-check.
Warm up

9
After self-test, the instrument goes into pre-warm state. For accurate test, at least 30
minutes of warm up is required.
Check the cuvettes
The cuvettes must be clear and there’s no remains of the samples on the surface of it.
Photometry
1. Enter into Photometry
“Main menu” (left) Enter “Basic”.
----
----
2. Set Wavelength
Press to set wavelength, press , to input wavelength value, press
(left) to go to the reference value.
GOTOλ
3. Blank
Put the blank cuvette into the light path, then press to do blank.

10
4. Test sample
Put the sample into light path, press (left) to test, the result will be displayed in the
data list, repeat this step to test all the samples.
5. Print Test Results
Press the key , , select “Print, clear data”, press key
(left) to print.
Photometry
Date&Time: mm-dd-yyyy, hh:mm:ss
Model: V-1200
Serial No.: VECXXXXXXX
irmware Version: 2.5.0
VWR International bvba.
No. WL.(nm) Abs %T
1 500.0 0.000 100.0
2 500.0 0.000 100.0
3 500.0 0.000 100.0
End.
Quantitative ode
1. Enter into Quantitative Mode
“Main menu” , press (right) to enter into “Quantitative”.
----
----

11
2. Build standard curve or use the saved standard curve
There are two methods to build standard curve, you can choose one method according to
your situation:
Input Coefficient:
1) Choose Coefficient. Press , to choose “build standard curve”,
press , to choose ”coefficient”.
----
----
2) Set wavelength. Press , to input test wavelength value, press
(left) to confirm.
3) Set test coefficient K. Press , to input test coefficient K, press
(left)to confirm.
----
----
4) Set test coefficient B. Press , to input test coefficient B value,

12
press (left)to confirm to finish setting of this method, the curve will be
displayed in the screen.
Coefficient K:
00000
WL.: 500.0 nm
Coefficient B:
+00000
WL.: 500.0 nm
K Cancel K Cancel
(L)
(R)
----
----
Create Standard Curve:
1) Choose Create Curve. Press , to choose ”Create Curve ”,
press , to choose “Standard Curve”, press (left) to enter
into.
----
----
2) Set wavelength. Press to enter to set wavelength, Press ,
to input wavelength value, press (left) to go the setting value.
GOTOλ

13
3) Blank. Pull the blank sample in light path, press (left) to do blank.
4) Input the number of standards. Press , to input the quantity of
standard sample(Not more than 9 standard curve), press (left) to
confirm.
----
----
5) Input the concentration of standards. When you have finished the last step,
the system will ask you to input the standards’ sample in the light path, press
, to input the concentration, press (left) to confirm, to
finish al the standard sample. It will appear a Save Information. press ,
to input the name, press (left) to confirm, and press
(left) again to finish save.
Number: 3
WL.: 500.0 nm 0.000Abs
Insert 1# Standard.
Input 1# Conc: 00000
WL.: 500.0 nm 0.107Abs
K Cancel K Cancel
(L)
(R)
----
----
Load curve:

14
Press , to choose ”Load Curve”, press , to
choose the cursor, press (left)to confirm.
----
----
Press (left) to enter the test mode after building or loading standard
curve.
----
3. Blank.
Put reference into light path, press to do blank.
4. Using standard curve to test sample.
Put samples into light path, Press (left) to test result will be displayed in the data
list. Repeat this step to finish all samples test.
5. Print Test Results
Press the key , , select “Print, clear data”, press key