VWR avantor 76359-754 User manual

VWR® Real-Time Electrophoresis Tank System
North American Catalog Number: 76359-754
European Catalog Number: 700-1472
This product is for research use only
Version 1
Issued: 08, 2021
Instruction manual

Legal Address of Manufacturer:
Country of origin: China
Europe
VWR International bv,
Researchpark Haasrode 2020
Geldenaaksebaan 464 B-3001 Leuven
[phone]
be.vwr.com
UK Importer:
VWR International Ltd
Hunter Boulevard, Magna Park
Lutterworth, Leicestershire, LE17 4XN
uk.vwr.com
United States
VWR International LLC
100 Matsonford Rd
Radnor, PA 19087
[phone]
www.vwr.com

Thank you for purchasing the VWR Real-Time Electrophoresis Tank System.
This operating manual includes a product introduction and operating and safety
information. Before using the Real-Time Electrophoresis Tank System, please read this
manual in its entirety and be sure to fully understand the features and methods for
proper operation. Keep this manual for future reference.
Please check the list of included components in section 1.1, and if there are any parts
missing, damaged, or incorrect, please contact your distributor or VWR’s Customer
Service Department.
Table of Contents
1. Introduction………………………………………....................2
1.1 Included Component List…….………….…………….……..3
1.2 Diagrams of Included Components...................................4
1.3 Specifications.………………………….….............................6
2. Installation….…………………………………..……………….6
2.1 Intended Use …………………………………………….……..6
3. Warnings……………..………………………..........................7
4. Gel Preparation.……………………………….……...….……8
5. Gel Tank Set Up ………………………………………………10
6. Electrophoresis ..………………………………….....…...….12
7. Viewing Electrophoresis Progress…….……….……….…12
8. Imaging using a smart phone…..…….……….…..….…….13
9. Maintenance……………………………………….…….……..13
10. Troubleshooting………………….…..….............................14
11. Equipment Disposal …………………………………………14
12. Technical Service and Warranty …………………………..15
13. Local VWR offices in Europe and Asia Pacific ..……….16
1. Introduction
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The Real-Time Electrophoresis Tank System is a horizontal electrophoresis
system that includes a gel running tank, safety lid with viewing filter, blue LED
transilluminator, and a gel casting set. The system is designed to connect to
an appropriate electrophoresis power supply for separating nucleic acids in
agarose gels and allows viewing the stained DNA bands during
electrophoresis without removing the gel from the tank.Accessories are
included for casting the agarose gels.
The blue LED transilluminator and viewing filter are compatible with green
fluorescent stains such as VWR’s EZ Vision Products, SYBR® Green, and
other similar stains that fluoresce under 465nm blue light.
Before use, please read this operating manual in its entirety.
1.1 Included Components
Gel Running Tank Assembly 1pc
Blue Light LED Illuminator 1 pc
12VDC Power Supply (for LED Illuminator) 1 pc
Gel Casting Stand 1 pc
Gel Casting Divider 1 pc
Long Gel Tray (10.5 x 10cm) 1 pcs
Short Gel Tray (10.5 x 6cm) 2 pcs
Double Sided Comb (12/22 teeth) 2 pcs
User Manual 1 pc
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Safety Lid with Integral Viewing
Filter and Power Leads
Gel Running Tank
Blue LED Transilluminator
12V Power Supply
Smart phone imaging
enclosure with photo filter
1.2 Diagrams of Included Components
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Diagram of Included Components (Gel Casting Set)
Double Sided Comb (12/22 teeth)
Long Gel Tray (10.5 x 10cm)
Short Gel Trays (10.5 x 6cm)
Gel Casting Divider
Gel Casting Stand
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1.3 Product Specifications
Migration Tank
Dimension 120mm×110mm×45mm（inner dimension）
Buffer volume 200ml-225ml
Electrodes Platinum wire
Blue LED Transilluminator
Viewing area
dimension 10.5cm x 10.5cm
Wavelength 465nm
Input voltage 12VDC (Power Adapter included for 100VAC to
240VAC input)
Gel Casting Set
Gel casting stand Accommodates 10.5cm x 11cm and
10.5cm×6cm trays (Divider included)
Comb
specifications
Teeth width x Teeth thickness x Teeth no.）
3mm×1mm×22
5.6mm×1mm×12
Gel trays 10.5cm x 11cm and 10.5cm×6cm
2. Installation
Place the electrophoresis tank on a smooth, level surface. Confirm that the power
supply to be used is suitable for the voltage and amperage requirements for DNA
electrophoresis and that the output jacks on the power supply can accept the
banana plugs on the power cords. The VWR 500V/400mA and VWR Mini Power
Supply are recommended as compatible power supplies.
2.1. Intended Use
This electrophoresis system is designed for use in a research laboratory for the
application of separating nucleic acid molecules in agarose gels.
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3. Warnings
This product is capable of delivering potentially lethal voltage when
connected to a high-powered power supply and should be operated
only by qualified and trained personnel.
Please carefully read this instruction manual before operation to avoid
any personal injury. Only trained laboratory personnel should operate
the system.
Do not attempt to open or repair any parts of the system. Contact
your distributor or Benchmark Scientific for service.
Always use the system in an environment with low humidity and low dust
, also keep away from water, direct sunlight / strong light, corrosive gas,
high magnetic fields, heaters, fires and other heat sources.
Do not detach the power supply cords from the safety lid and do not
remove the safety lid when the attached power supply is running.
Attempting to do so can cause damage and can also create a shock
hazard.
Use only appropriate power supplies that are designed for
electrophoresis applications and have safety features such as no load
and overload detection.
When operating, do not move or bump the system, do not put your finger
or any other objects into the migration tank when connected to a running
power supply.
The blue light transilluminator produces a high intensity light, and users
should not stare directly at the light.
Symbols and Conventions
CAUTION This symbol indicates a potential risk and alerts you to
proceed with caution
CAUTION This symbol indicates the presence of high voltage and
warns the user to proceed with caution
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4. Gel Preparation
Place the gel casting stand on a level surface
and place the divider and desired gel trays
into the proper positions in the stand.
Either one long gel tray, or two short trays can
be inserted into the casting stand (see
diagrams on right).
Note: If the gel tray is not level, the thickness
of the gel will not be uniform and DNA
migration may be uneven.
Insert the comb(s) into the gel casting
stand as per image on the right. Multiple
combs can be inserted depending on the
number of samples and desired run
distance.
Note: when using the fine-toothed
comb, the flat side of the well comb
should be facing the direction of
DNA travel. See figure below.
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Pour the agaose gel/DNA stain
solution into the gel tray to make a
gel approximately 4mm thick.
Each 6x10.5cm tray requires
about 25ml of agarose.
Note: VWR’s EZ Vision Products
are recommended for gel stain.
After the gel has solidified
(approximately 20 minutes) hold the
two sides of comb and gently lift it out
of the gel. The wells should be
straight and undamaged.
Carefully remove the gel tray with the
gel from the stand and place it into
the migration tank in the correct
orientation. (DNA samples will
migrate from the –to + electrode).
Note: After mixing and heating
agarose solution, let it cool to
approximately 60°C before
pouring into the gel trays
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Note: Make sure that the tank is installed
on a stable and level surface. This will
ensure even sample migration.
Place a prepared gel, together with the gel
tray onto the gel bed inside the gel tank.
Carefully pour an appropriate buffer solution to
a level of about 2 mm above the surface of the
gel. Typically, 200ml of buffer is required.
It is recommended to use TAE or TBE at a
concentration of 0.5X.
5. Gel Tank Set Up
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Add DNA samples to the gel
Use an appropriate pipette to
carefully dispense samples into the
wells of the gel.
Note: Gel loading buffer should be
mixed with the DNA samples so they
will sink to the bottom of the wells.
Carefully lower the safety cover onto the
gel tank. The orange viewing filter should
fully contact the surface of the buffer so
there are no bubbles visible. If the buffer
level is too low to contact the orange
filter, remove the cover, and add more
buffer to the tank.
Slide the blue LED illuminator under
the gel tank.
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Connect the
12V power
supply to the
LED
transilluminator
6. Electrophoresis
7. Viewing Electrophoresis Progress
To visualize the DNA separation in
the gel during electrophoresis, turn
on the blue LED illuminator using
the ON/OFF switch.
It may be necessary to dim or turn
off ambient lights for best viewing
results.
The LED illuminator has a built-in
timer, so the light will automatically
switch off after 5 minutes.
Connect the positive (red) and negative
(black) power leads from the safety
cover to the + and –outputs of a power
supply.
Set the power supply output voltage
appropriately for the application.
Generally 100V with constant voltage
setting is acceptable for most
applications
Connect the included 12V DC power
supply to the inlet on the transilluminator.
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8. Imaging using a smart phone
To take a picture of the gel using a
smart phone camera, fit the imaging
enclosure onto the lid of the gel
running tank.
Insert the included orange photo filter
into the top platform of the enclosure.
Turn on the smart phone’s camera
application and align the camera lens
with the photo filter.
Zoom and focus as necessary to
optimize the image and take the
picture.
9. Maintenance
Always disconnect the 12V power supply when cleaning the
transilluminator. When cleaning the surfaces of the
instrument and LED transilluminator, use a damp cloth with
mild detergent if needed. Do not use any corrosive solutions
that could damage plastic.
When cleaning the gel tank, use caution when cleaning the areas
near the platinum electrodes at the bottom edges of the gel tank.
Clean gel casting stands, gel tray and combs with water or
a neutral cleaner.
Do not submerge the LED transilluminator in water.
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10. Troubleshooting
Problem Causes Resolutions
No Migration
Power supply not
operating or not
correctly connected, or
gel inserted backwards
Check the proper operation of the
power supply and proper connection
of the power leads to + and - outputs.
Check that the gel is properly oriented
(DNA will migrate towards + electrode)
Abnormal
Electrophoresis
speed.
Incorrect voltage
setting, incorrectly
prepared buffer.
Check power supply output, check
that buffer is the proper type and
appropriate concentration.
Abnormal heating of
buffer or gels
Buffer concentration
too high, voltage too
high, or buffer has
been reused too many
times.
Use 0.5X TAE/TBE buffer or reduce
running voltage. If buffer has been
used in previous runs, replace with
freshly prepared buffer.
11. Equipment disposal
This equipment is marked with the crossed out wheeled bin
symbol to indicate that this equipment must not be
disposed of with unsorted waste.
Instead it's your responsibility to correctly dispose of your
equipment at lifecycle -end by handling it over to an
authorized facility for separate collection and recycling. It's
also your responsibility to decontaminate the equipment in
case of biological, chemical and/or radiological
contamination, so as to protect from health hazards the
persons involved in the disposal and recycling of the
equipment.
For more information about where you can drop off your
waste of equipment, please contact your local dealer from
whom you originally purchased this equipment.
By doing so, you will help to conserve natural and
environmental resources and you will ensure that your
equipment is recycled in a manner that protects human
health. Thank you
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12. Technical Service and Warranty
Web Resources
Visit the VWR website at www.vwr.com for:
• Complete technical service contact information
• Access to the VWR Online Catalogue, and information about accessories and related
products
• Additional product information and special offers
Contact us For information or technical assistance contact your local VWR
representative or visit. www.vwr.com.
Warranty
VWR warrants that this product will be free from defects in material and workmanship
for a period of two (2) years from date of delivery. If a defect is present, VWR will, at its
option and cost, repair, replace, or refund the purchase price of this product to the
customer, provided it is returned during the warranty period. This warranty does not
apply if the product has been damaged by accident, abuse, misuse, or misapplication,
or from ordinary wear and tear. If the required maintenance and inspection services are
not performed according to the manuals and any local regulations, such warranty turns
invalid, except to the extent, the defect of the product is not due to such non-
performance.
Items being returned must be insured by the customer against possible damage or loss.
This warranty shall be limited to the aforementioned remedies. IT IS EXPRESSLY
AGREED THAT THIS WARRANTY WILL BE IN LIEU OF ALL WARRANTIES OF
FITNESS AND IN LIEU OF THE WARRANTY OF MERCHANTABILITY.
Compliance with local laws and regulations. The customer is responsible for
applying for and obtaining the necessary regulatory approvals or other authorisations
necessary to run or use the Product in its local environment. VWR will not be held liable
for any related omission or for not obtaining the required approval or authorisation,
unless any refusal is due to a defect of the product.
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Austria
VWR International GmbH
Graumanngasse 7
1150 Vienna
Tel.: [phone]
Email: [email protected]
Belgium
VWR International BV
Researchpark Haasrode 2020
Geldenaaksebaan 464
3001 Leuven
Tel.: [phone]
Email: [e-mail]
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Mississauga, Ontario L5N 5Z7
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Shanghai, 201203- China
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(Northern Ireland) Ltd
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LE17 4XN
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100 Matsonford Road
Building One Suite 200
Radnor, PA 19087
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vwr[email protected]
13. Our local offices in Europe, AMEA and North America