YSI PRO20 User manual

PRO20
USER MANUAL
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Item # 605597
Rev B
Drawing # A605597
October 2008
©2008 YSI Incorporated.
The YSI logo is a registered trademark of YSI Incorporated.
Teflon is a registered trademark of E. I. du Pont de Nemours and Company.

CONTENTS
Warranty .....................................................................................................1
Introduction................................................................................................2
Getting Started ............................................................................................2
Initial Inspection................................................................................2
Battery Installation.............................................................................2
Keypad................................................................................................3
Connecting the Sensor and Cable.....................................................4
Membrane Installation ......................................................................7
Backlight.............................................................................................7
Powering Off ......................................................................................7
Run Screen .........................................................................................7
Navigation ..........................................................................................8
First Power On ...................................................................................9
System Setup Menu ..................................................................................10
DO Local%.......................................................................................10
Last Digit Suppression (LDS)..........................................................11
One Touch Calibration (One Touch Cal)......................................11
Audio ................................................................................................11
Contrast............................................................................................11
Sensor Type ......................................................................................12
Membrane Type...............................................................................13
Auto Stable .......................................................................................14
DO Units ..........................................................................................14
Temperature Units...........................................................................15
Pressure Units ..................................................................................15
Language...........................................................................................15
Auto Shutoff.....................................................................................15
Resetting the System Setup Menu to Factory Default ...................16
Exiting the System Setup Menu ......................................................17

Calibration ................................................................................................17
Temperature.....................................................................................17
Barometer.........................................................................................17
Dissolved Oxygen.............................................................................18
Salinity Compensation Calibration ................................................21
Taking Measurements ..............................................................................22
Saving and Viewing data...........................................................................22
Saving Data.......................................................................................22
Viewing and Erasing Saved Data – Data mode ..............................23
Principles of Operation ............................................................................25
Care, Maintenance, and Storage ..............................................................26
General Maintenance.......................................................................26
Sensor Maintenance.........................................................................28
Sensor Storage..................................................................................32
Troubleshooting........................................................................................33
Specifications.............................................................................................35
Accessories/Part Numbers........................................................................36
Declaration of Conformity.......................................................................37
Recycling....................................................................................................38
Contact Information.................................................................................39
Ordering & Technical Support........................................................39
Service Information .........................................................................39

WARRANTY
The YSI Pro20 Instrument is warranted for three (3) years from date of purchase by the end
user against defects in materials and workmanship, exclusive of batteries and any damaged
caused by defective batteries. Pro20 cables are warranted for two (2) years from date of
purchase by the end user against defects in material and workmanship. Pro20 Polarographic
sensors are warranted for one (1) year and Galvanic sensors are warranted for six (6)
months from date of purchase by the end user against defects in material and workmanship.
Pro20 instruments, cables & probes are warranted for 90 days from date of purchase by the
end user against defects in material and workmanship when purchased by rental agencies
for rental purposes. Within the warranty period, YSI will repair or replace, at its sole
discretion, free of charge, any product that YSI determines to be covered by this warranty.
To exercise this warranty, call your local YSI representative, or contact YSI Customer
Service in Yellow Springs, Ohio at [phone], 800-897-4151 or visit www.ysi.com
(Support tab). Send the product and proof of purchase, transportation prepaid, to the
Authorized Service Center selected by YSI. Repair or replacement will be made and the
product returned, transportation prepaid. Repaired or replaced products are warranted for
the balance of the original warranty period, or at least 90 days from date of repair or
replacement.
LIMITATION OF WARRANTY
This Warranty does not apply to any YSI product damage or failure caused by:
1) failure to install, operate or use the product in accordance with YSI's written
instructions;
2) abuse or misuse of the product;
3) failure to maintain the product in accordance with YSI's written instructions
or standard industry procedure;
4) any improper repairs to the product;
5) use by you of defective or improper components or parts in servicing or
repairing the product;
6) modification of the product in any way not expressly authorized by YSI.
THIS WARRANTY IS IN LIEU OF ALL OTHER WARRANTIES, EXPRESSED OR
IMPLIED, INCLUDING ANY WARRANTY OF MERCHANTABILITY OR FITNESS FOR
A PARTICULAR PURPOSE. YSI's LIABILITY UNDER THIS WARRANTY IS LIMITED
TO REPAIR OR REPLACEMENT OF THE PRODUCT, AND THIS SHALL BE YOUR
SOLE AND EXCLUSIVE REMEDY FOR ANY DEFECTIVE PRODUCT COVERED BY
THIS WARRANTY. IN NO EVENT SHALL YSI BE LIABLE FOR ANY SPECIAL,
INDIRECT, INCIDENTAL OR CONSEQUENTIAL DAMAGES RESULTING FROM ANY
DEFECTIVE PRODUCT COVERED BY THIS WARRANTY.
1

INTRODUCTION
Thank you for purchasing the YSI Pro20, an instrument from the YSI Professional
Series product family. The Pro20 features an impact resistant and waterproof (IP-
67) case, backlit display, user-selectable sensor options, internal barometer, and a
rugged, rubber over-mold case.
The Pro20 provides valuable instructions and prompts near the bottom of the
display that will guide you through operation and use. However, reading the
entire manual is recommended for a better understanding of the Pro20’s features.
The Pro20 can not communicate to a PC via a Pro Plus
communications saddle. Connecting the Pro20 to a
communication saddle may cause erratic instrument behavior.
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GETTING STARTED
INITIAL INSPECTION
Carefully unpack the instrument and accessories and inspect for damage.
Compare received parts with materials listed on the packing list. If any parts or
materials are missing or damaged, contact YSI Customer Service at 800-897-4151
([phone]) or the Authorized YSI distributor from whom the instrument
was purchased.
BATTERY INSTALLATION
This instrument requires 2 alkaline C-cell batteries. Under normal conditions,
battery life is approximately 400 hours at room temperature without using the
back light. A battery symbol will blink in the lower, left corner of the
display to indicate low batteries when approximately 1 hour of battery life
remains.
To install or replace the batteries:
1) Turn the instrument off and flip over to view the battery cover on the back.
2) Unscrew the four captive battery cover screws.
3) Remove the battery cover, and remove the old batteries if necessary.
4) Install the new batteries, ensuring correct polarity alignment (Figure 1).
2

5) Place the battery cover on the back of the instrument and tighten the four
screws. Do NOT over-tighten.
The waterproof instrument case is sealed at the factory and is not to
be opened, except by authorized service technicians. Do not
attempt to separate the two halves of the instrument case as this
may damage the instrument, break the waterproof seal, and will
void the warranty.
KEYPAD
L
3
4
5
6
1
2
Figure 1, Pro20 with battery cover removed. Note battery symbols indicating
polarities.
Figure 2, keypad
3

Number Key Description
1
Calibrate
Press and hold for 3 seconds to calibrate.
Initiates One Touch Calibration.
Opens Calibrate menu from the run screen if One
Touch Calibration is disabled.
2
Up Arrow
Use to navigate through menus, to navigate through
box options at the bottom of the run screen, and to
increase numeric inputs.
3
Power and Backlight
Press once to turn instrument on. Press a second
time to turn backlight on. Press a third time to turn
backlight off. Press and hold for 3 seconds to turn
instrument off.
4
Menu
Use to enter the System Setup menu from the run
screen.
5
Enter
Press to confirm entries and selections.
6
Down Arrow
Use to navigate through menus, to navigate through
box options at the bottom of the run screen, and to
decrease numeric inputs.
CONNECTING THE SENSOR AND CABLE
CONNECTING THE SENSOR
“Sensor” refers to the removable portion or electrode sensing portion of the cable
assembly, i.e. the dissolved oxygen sensor. “Bulkhead” refers to the portion of the
cable with the single-pin connector (Figure 3).
4

The Pro20 has two compatible sensors for use with a field cable:
Polarographic – This sensor has a black sensor body and is engraved with the
model number 2003. Polarographic will be abbreviated Polaro in the instrument.
Galvanic – This sensor has a grey sensor body and is engraved with the model
number 2002.
For information about the differences on the two sensor types, see Sensor Type in
the System Setup menu section and/or the Principles of Operation section of this
manual.
If using a ProBOD sensor/cable assembly, there is no need to install a sensor
because it has a built in Polarographic dissolved oxygen sensor.
Before installing either sensor or connecting the cable to the
instrument, the Sensor Type must be configured for the sensor being
installed/connected. Failure to do this may result in damage not
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covered under warranty. The instrument will step you through this
setup the first time it is powered on. See the System Setup menu
section of this manual for instructions on configuring the Sensor
Type after the first power on.
1) Ensure both the sensor connector and sensor port on the cable are clean and
dry.
2) Grasp the sensor with one hand and the cable bulkhead in the other.
3) Push the sensor into the connector on the cable until it is firmly seated and
only 1 o-ring is visible. Failure to properly seat the sensor may result in
damage.
4) Twist the sensor clockwise to engage threads and finger tighten. Do NOT
use a tool. This connection is water-tight.
For more detailed instructions, please refer to the sensor installation sheet that is
included with each sensor.
Figure 3
Bulkhead
Sensor
5

CONNECTING THE CABLE
The Pro20 is designed for field and laboratory use. It is compatible with two
different cable options:
1) The field rugged cable is available in standard lengths of 1, 4, 10, 20, 30, and
100 meters with special lengths available between 30 and 100 meters. This
cable has a built in temperature sensor and includes a port for the dissolved
oxygen sensor.
2) The ProBOD is a 1 meter probe/cable assembly with built in Polarographic
dissolved oxygen and temperature sensors. It has an AC powered motor for
sample stirring and is designed to fit into a 300 ml BOD bottle.
To connect the cable, align the keys in the cable connector to the slots in the
instrument connector. Push together firmly and then twist the outer ring until it
locks into place (Figure 4). This connection is water-proof.
Figure 4, Note the keyed connector.
When disconnected, the sensor and cable’s sensor connectors are
NOT water-proof. Do not submerge the cable without a sensor
installed. When disconnected, the cable’s instrument connector and
the connector on the instrument maintain a waterproof, IP-67
rating.
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6

MEMBRANE INSTALLATION
The dissolved oxygen sensor is shipped with a dry, protective red cap that will need
to be removed before using. It is very important to put a new membrane with
electrolyte solution on the sensor after removing the red cap.
Prepare the membrane solution according to the instructions on the bottle. After
mixing, allow the solution to sit for 1 hour. This will help prevent air bubbles
from later developing under the membrane. Ensure you are using the correct
electrolyte solution for the correct sensor. Galvanic sensors utilize electrolyte with
a light blue label and Polarographic sensors utilize electrolyte with a white label.
The Dissolved Oxygen sensor is supplied with cap membranes specific to the
sensor type ordered (Polarographic or Galvanic). 5913 and 5914 membrane kits
are for Galvanic sensors and the 5908 and 5909 membrane kits are for
Polarographic sensors.
Remove and discard or save the red protective cap. Thoroughly rinse the sensor tip
with distilled or deionized water. Fill the cap membrane 3⁄4 full of electrolyte
solution, then tap the cap with a finger to release any trapped air. Be careful not to
touch the membrane portion of the cap. Thread the membrane cap onto the
sensor, moderately tight. Do not use a tool. It’s typical for some of the electrolyte
solution to spill over. It is best to allow the new cap to remain on a new sensor
overnight before trying to calibrate. For detailed instructions on changing a
membrane cap, see the Care, Maintenance, and Storage section of this manual.
BACKLIGHT
Once the instrument is on, pressing power/backlight key will turn on the
display backlight. The backlight will remain on until the key is pressed again or
after two minutes of not pressing any key on the keypad.
POWERING OFF
To turn the instrument off, press and hold the power/backlight key for three
seconds.
RUN SCREEN
Press the power/backlight key to turn the instrument on. The instrument will
run through a self test and briefly display a splash screen with system information
before displaying the main run screen (Figure 5). The first time the Pro20 is
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turned on, it will step through language, sensor, and membrane selections; see the
First Power On section of this manual for more information.
8.04 mg/L
95.9 %
24.2 °C

SAVE

DATA
730.3
mmHg
0.0
ppt
Figure 5, example of main run screen with Save highlighted.
NAVIGATION
The up and down arrow keys allow you to navigate through the functions
of the Pro20.
NAVIGATING IN THE RUN SCREEN
When in the run screen, the up and down arrow keys will move the
highlighted box along the bottom options. Once a box is highlighted, press enter
to access the highlighted option.
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Description of run screen box functions from left to right:
Option Description
Barometer reading Highlight and press enter to calibrate the barometer
Salinity compensation
value
Highlight and press enter to adjust salinity
compensation value

SAVE Highlight and press enter to save current data to
memory

DATA Highlight and press enter to view and/or erase saved
data
NAVIGATING IN THE SYSTEM SETUP MENU
When in the System Setup menu, the up and down arrow keys will move the
highlighted bar up and down the system setup options. See the System Setup
menu section of this manual for more information about these options.
FIRST POWER ON
The instrument will step through an initial configuration when powered on for the
first time. This will set the language, sensor, and membrane options. Use the up
or down arrow keys to highlight the appropriate language, sensor, and membrane,
then press enter to confirm (Figures 6, 7, and 8). The Sensor Type must be
configured for the sensor installed. Failure to do this may result in damage not
covered under warranty. If an incorrect option is selected, it may be changed in
the System Setup menu.
Select Sensor Type:
7Polaro (black)
Galvanic (grey)
Use ST to select
sensor type
Press to confirm
Select Language:
7English
Français
Español
Deutsch
Use ST to select
Language
Press to confirm
Figure 6, Language selection
Select Membrane
Type:
71.25 (Yellow)
2.0 (Blue)
Use ST to select
membrane
Press to confirm
Figure 8, Membrane selection
Figure 7, Sensor selection
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After selecting a language, sensor, and membrane, the run screen will appear. The
next time the instrument is powered up the run screen will appear immediately
after the self check. If the sensor type or membrane type is changed, ensure that it
updated in the System Setup menu.
SYSTEM SETUP MENU
Press the menu key to access the following System Setup functions.
The System Setup menu contains multiple screens which are notated as ‘pages’.
The current page is indicated on the display, figure 9.
DO LOCAL%
DO Local% can be enabled or disabled by using the up or down arrow keys to
highlight it and then pressing enter. An ‘X’ in the box next to DO Local%
indicates it is enabled (Figure 9).
7DO Local%
LDS
One Touch Cal
Audio
Contrast
Sensor Type
Membrane Type
page 1 of 2
ESC 4
Reset
Exit
Figure 9, DO %Local is enabled.
When DO Local% is enabled, DO% values will be expressed as %L on the run
screen.
DO Local% allows for localized dissolved oxygen measurements. This sets the
DO% calibration value to 100% regardless of the altitude or barometric pressure.
When DO Local% is enabled, the Pro20 will factor in the barometric pressure on
each measurement. For example, if the barometric pressure changes, the DO %L
reading would remain constant in air-saturated water or in water-saturated air.
Local DO is ideal for EU compliance.
10

LAST DIGIT SUPPRESSION (LDS)
Last Digit Suppression (LDS) can be enabled or disabled by using the up or down
arrow keys to highlight it and pressing enter . An ‘X’ in the box next to LDS
indicates it is enabled.
LDS rounds the DO value to the nearest tenth; i.e. 8.25 mg/L becomes 8.3 mg/L.
LDS is automatically disabled during calibrations.
ONE TOUCH CALIBRATION (ONE TOUCH CAL)
One Touch Calibration can be enabled or disabled by using the up or down arrow
keys to highlight One Touch Cal and pressing enter . An ‘X’ in the box next to
One Touch indicates it is enabled.
When One Touch Cal is enabled, press and hold the calibration key for 3
seconds while in the run screen to calibrate Dissolved Oxygen to the barometer
reading and salinity correction value. For more information on One Touch
Calibration, see the Calibration section of this manual.
AUDIO
Audio can be enabled or disabled by using the up or down arrow keys to highlight
Audio and pressing enter . When enabled, there will be an ‘X’ in the box next
to Audio.
When Audio is enabled, the Pro20 will beep twice to indicate stability when Auto
Stable is enabled. The instrument will also beep when a key is pressed. When
Audio is disabled, the Pro20 will not beep.
CONTRAST
To adjust the display Contrast, use the up or down arrow keys to highlight
Contrast, then press enter . Next, use the up or down arrow keys to adjust the
contrast. The up arrow will darken the contrast and the down arrow will lighten
the contrast. After adjusting the contrast, press enter to save and exit the Contrast
adjustment option.
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EMERGENCY CONTRAST ADJUSTMENT
If necessary, there is an alternate method of adjusting the contrast. To adjust the
contrast, press and hold the menu key, then press the up arrow key to darken the
contrast or press the down arrow key to lighten the contrast.
SENSOR TYPE
The instrument’s Sensor Type must be configured for the sensor
installed. Failure to do this may result in damage not covered
under warranty. If you observe readings very close to 0 or
extremely high readings, i.e. 600%, your Sensor Type setting may
be set incorrectly.
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Sensor Type sets the type of oxygen sensor being used; either Polarographic
(black) or Galvanic (grey).
Use the up or down arrow keys to highlight Sensor Type, then press enter to
open a submenu. Highlight the sensor type corresponding to the sensor installed
on the cable and press enter to confirm. The enabled sensor type will have an ‘X’
in the box next to it. Use the down arrow key to highlight the ESC – Exit, then
press enter to save changes and to close the sensor submenu.
If using a ProBOD sensor/cable assembly, the sensor type should be set to
polarographic.
The Pro20 has two compatible sensors for use with a field cable:
Polarographic – This sensor has a black sensor body and is engraved with the
model number 2003. Polarographic will be abbreviated Polaro in the instrument.
Galvanic – This sensor has a grey sensor body and is engraved with the model
number 2002.
In terms of physical configuration, membrane material, and general performance,
YSI Professional Series Galvanic dissolved oxygen sensors are exactly like the
Professional Series Polarographic sensors. The advantage of using Galvanic
sensors is convenience. Galvanic sensors provide for an instant-on sensor without
the need for warm-up time but this affects the life of the sensor. Polarographic
sensors last longer and have a longer warranty but require a 5-15 minute warm-up
time before use or calibration.
12

MEMBRANE TYPE
Membrane Type sets the type of membrane used on the dissolved oxygen sensor;
either 1.25 PE (Yellow) or 2.0 PE (blue). Use the up or down arrow keys to
highlight Membrane Type and press enter to open the membrane submenu.
Highlight the membrane type corresponding to the membrane installed on the
sensor and press enter to confirm. The enabled membrane type will have an ‘X’ in
the box next to it. Use the down arrow key to highlight the ESC – Exit box and
press enter to save changes and to close the membrane submenu.
The dissolved oxygen sensor is supplied with membranes specific to the sensor
type ordered and are color coded as described in the following tables.
Galvanic Membrane Kits
Item Color Material Description
5913 Yellow 1.25 mil polyethylene Faster response time and less flow
dependence than traditional Teflon®
membranes
5914 Blue 2.0 mil polyethylene Less flow dependence than 1.25 mil
but somewhat slower response
Polarographic Membrane Kits
Item Color Material Description
5908 Yellow 1.25 mil polyethylene Faster response time and less flow
dependence than traditional Teflon®
membranes
5909 Blue 2.0 mil polyethylene Less flow dependence than 1.25 mil
but somewhat slower response
Selecting a Dissolved Oxygen Membrane
Membrane Type Flow Dependence After 4 Minutes Typical Response Time
to 95%
5913, 5908 - Yellow 25% 8 seconds
5914, 5909 – Blue 18% 17 seconds
13

AUTO STABLE
Auto Stable utilizes preset values to indicate when a reading is stable. The preset
values are adjustable in the System Setup menu. The user can input a % change in
dissolved oxygen readings (0.0 to 1.9) over ‘x’ amount of time in seconds (3-19).
Highlight Auto Stable and press enter to expand the submenu. Use the up or
down arrow keys to highlight the DO% Change or seconds (secs) input field, then
press enter to make the highlighted field adjustable. Use the up and down arrow
keys to adjust the selected value, then press enter to confirm changes. Once you
have confirmed any changes, highlight the ESC-Exit box and press enter to close
the Auto Stable submenu.
To disable Auto Stable, set the DO% Change input to 0.0.
When Auto Stable is enabled, a will display next to the dissolved oxygen
value on the run screen and blink during stabilization. When the dissolved oxygen
value has stabilized based on the Auto Stable settings, the will display steadily
and the instrument will beep twice if Audio is turned on.
DO UNITS
Highlight DO Units and press enter to open a submenu that will allow you to
select the dissolved oxygen units displayed on the run screen. Highlight the
desired unit(s) and press enter to enable or disable. An enabled dissolved oxygen
unit will have an ‘X’ in the box next to it. Highlight the ESC-Exit box and press
enter to save any changes and to close the DO units submenu.
There are three options for displaying dissolved oxygen:
mg/L will show DO readings in milligrams per liter on a scale
from 0 to 50 mg/L.
ppm (parts per million) is equivalent to mg/L and will show the
DO reading on a scale from 0 to 50 ppm.
%will show DO readings in a percent scale from 0 to 500%.
This value will be expressed %L when DO Local% is enabled.
Both % or %L and mg/L or ppm can be displayed simultaneously on the screen.
14

15
TEMPERATURE UNITS
Highlight Temperature Units and press enter to open a submenu that will allow
you to change the temperature units displayed in the run screen. Highlight the
desired unit (Celsius or Fahrenheit) and press enter to enable. The enabled
temperature unit will have an ‘X’ in the box next to it. Only one unit may be
enabled at a time. Highlight the ESC-Exit box and press enter to save any changes
and to close the Temperature Units submenu.
PRESSURE UNITS
Highlight Pressure Units and press enter to open a submenu that will allow you to
change the units displayed on the run screen. Highlight the desired unit (mmHg,
inHg, mbar, psi, or kPa) and press enter to enable. The enabled pressure unit will
have an ‘X’ in the box next to it. Only one unit may be enabled at a time.
Highlight the ESC-Exit box and press enter to save any changes and to close the
Pressure Units submenu.
LANGUAGE
Highlight Language and press Enter to open a submenu that will allow you to
change the language. Highlight the desired language (English, Spanish, German,
or French) and press enter to enable. The enabled language will have an ‘X’ in the
box next to it. Highlight ESC-Exit box and press enter to save any changes and to
close the Language submenu.
The text in the boxes along the bottom of the run screen will always be displayed
in English regardless of the language enabled in the System Setup menu.
AUTO SHUTOFF
Auto Shutoff allows you to set the instrument to turn off automatically after a
period of time. Use the up or down arrow keys to highlight Auto Shutoff, then
press enter to open the submenu. Press enter while the minute field is highlighted
to make it adjustable. Next, use the up and down arrow keys to adjust the shut off
time from 0 to 60 minutes. Press enter to confirm and save the new shutoff time.
Highlight ESC-Exit box, then press enter to close the Auto Shutoff submenu.
To disable Auto Shutoff, set the Time in Minutes to 0 (zero).

RESETTING THE SYSTEM SETUP MENU TO FACTORY DEFAULT
To reset the Pro20 settings to factory default, press the down arrow key until the
Reset - 4box is highlighted, then press enter. The instrument will ask you to
confirm the reset. Highlight Yes and press enter to continue with the reset or
highlight No and press enter to cancel the reset. A Factory Reset will not affect
data saved in the unit’s memory.
The following will be set in the Pro20 after performing a factory reset:
Parameter Reset Defaults
Temperature Units oC
Dissolved Oxygen Units mg/L and %
Pressure Units mmHg
Dissolved Oxygen Sensor Type Last Setting Confirmed
Membrane Type Last Setting Confirmed
Salinity Compensation Value 0.0 ppt
DO Local% Off
One Touch Cal On
Display Contrast Set to mid range
Auto Shutoff 30 minutes
Auto Stable Off (0.0 % Change and 10 secs)
LDS (Last Digit Suppression) Off
Audio On
Language English
Dissolved Oxygen Calibration Reset to factory default, 100% for
enabled membrane and sensor*
Barometer Calibration Reset to factory default*
*It is recommended to perform a barometer and dissolved oxygen calibration
after
p
erformin
g
a reset.
16