Agilent Technologies 1260 Infinity User manual

Agilent 1260 Infinity
Sample Capacity
Extension
User Manual

Agilent 1260 Infinity Sample Capacity Extension
Notices
© Agilent Technologies, Inc. 2010
No part of this manual may be reproduced in
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tronic storage and retrieval or translation
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Manual Part Number
G2257-90011
Edition
06/10
Printed in Germany
Agilent Technologies
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This product may be used as a
com-ponent of an in vitro diagnostic
sys-tem if the system is registered
with the appropriate authorities and
com-plies with the relevant regula-
tions. Otherwise, it is intended only
for gen-eral laboratory use.
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Safety Notices
CAUTION
A CAUTION notice denotes a haz-
ard. It calls attention to an operat-
ing procedure, practice, or the like
that, if not correctly performed or
adhered to, could result in damage
to the product or loss of important
data. Do not proceed beyond a
CAUTION notice until the indicated
conditions are fully understood and
met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could result
in personal injury or death. Do not
proceed beyond a WARNING
notice until the indicated condi-
tions are fully understood and
met.

Agilent 1260 Infinity Sample Capacity Extension User Manual 3
In this Manual, you will find ...
1Overview
An overview of the Sample Capacity Extension system and its
capabilities.
2 Installation
Complete instructions for the installation and set-up of the
Well-plate handler and Automation Interface, and the optional
barcode readers and rack extension.
3 Software Navigation
A description of the set-up panels and dialog boxes for the
Sample Capacity Extension.
4 Working with the Sample Capacity Extension
A description of the features of the ChemStation that are
specific to the Sample Capacity Extension, with examples of
their use.
5 Troubleshooting and Error Handling
Information on the available maintenance and test functions,
with a list of possible errors and how to fix them.
A Safety Information
Important information on symbols and labels that appear on
the product or in the documentation.

4 Agilent 1260 Infinity Sample Capacity Extension

Agilent 1260 Infinity Sample Capacity Extension User Manual 5
Contents
1Overview
Description 10
Specifications 11
General Instrument 11
Robotic Arm 12
Physical Performance 12
Environmental 12
Physical 13
Electrical 14
Barcodes 14
2 Installation
Site Preparation 16
Bench Space Requirements 16
Contents of the G2257A Shipping Kit 17
Installation 19
Installing the Automation Interface 19
Setting Up the Well-plate Handler 25
Installing the Optional Rack Extension 33
Installing the Optional Fixed Barcode Reader 35
Installing the Optional Hand-held Barcode Reader 38
Barcode Requirements 39
Making the Electrical Connections 41
Adjusting the Barcode Reader Position 42
Adjusting the Lateral Position of the Well-plate Handler 44
Preliminary Adjustment of the Well-plate Handler Position 45

6 Agilent 1260 Infinity Sample Capacity Extension User Manual
Contents
Well-plate Handler Teaching 47
Teaching using the ChemStation 47
Fine Adjustment of the Well-plate Handler Position 51
Adjusting the radius 51
Adjusting for the gripper fingers 52
Adjusting the centering spring 53
De-Installation of the Automation Interface 54
Installing a new Automation Interface 55
3 G2257A Software Navigation
Using the Sample Capacity Extension with the
Agilent ChemStation 58
Well-plate Handler Configuration 58
4 Working with the Sample Capacity Extension
What is a Hypersequence? 64
Creating a Hypersequence 65
Hypersequence Parameters 65
Barcode Settings 69
The Hypersequence Table 70
Importing an Import File 72
Making an Import File 76
5 Troubleshooting and Error Handling
Well-plate Handler Test 82
Testing with the Agilent ChemStation 82
Automation Interface Test 85
Testing with the ChemStation 85
Solving Problems 87
Well-plate Handler does not turn on when switched on. 87

Contents
Agilent 1260 Infinity Sample Capacity Extension User Manual 7
The Well-plate Handler arm does not move down far enough to
depress the plate sensor switch. 87
Error Messages 88
AI Door Error 88
Plate Error 88
AI Door Sensor Error 88
Power Failure on AI 89
High Performance autosampler Thermostat Enabled Error 89
Maintenance 90
Cleaning the Well-plate Handler 90
Changing the Fuse(s) 91
Moving the Well-plate Handler 92
Long-Term Shutdown 93
Parts List 94
A Safety Information
Safety Information 98
General 98
Operation 98
Safety Symbols 99
Equipment Labels 100
Warning and Caution Notes in this Manual 100
Radio Interference 102
Agilent Technologies on Internet 103
Index 105

8 Agilent 1260 Infinity Sample Capacity Extension User Manual
Contents

9
Agilent 1260 Infinity Sample Capacity Extension
User Manual
Agilent Technologies
1
Overview
Description 10
Specifications 11
Figure 1 The G2257A Sample Capacity Extension

10 Agilent 1260 Infinity Sample Capacity Extension User Manual
1Overview
Description
The Agilent 1260 Infinity Sample Capacity Extension is a flexible and
upgradeable robotic well-plate loading system. The basic unit, comprising a
Well-plate Handler and an Automation Interface, is capable of sequentially
loading up to twenty 96-well or 384-well plates into the high performance
autosampler.
The Agilent 1260 Infinity Sample Capacity Extension works in conjunction
with the high performance autosampler and either fully automatically with
the Agilent ChemStation or interactively with the Agilent Instant Pilot
G4208A. The Well-plate Handler moves sample well-plates from an input rack
to the Automation Interface, which moves the well-plate into position in the
high performance autosampler. When the sequence is complete, the
Automation Interface collects the well-plate from the high performance
autosampler, and the Well-plate Handler retrieves the plate and places it into
an output rack. This process is repeated for up to 20 well-plates (expandable
to 80) in a single run.
To prevent sample contamination, well-plates with lids can be used, or closing
mats can be used to prevent evaporation. The Well-plate Handler removes the
lid prior to processing the plate, then replaces the lid after processing.
Alternatively, a lid or an additional empty well-plate can be used as a “topper”
on the stack to prevent contamination or evaporation of the top well-plate.
The Well-plate Handler removes the topper prior to processing the stack, then
replaces the topper on the stack.

Agilent 1260 Infinity Sample Capacity Extension User Manual 11
Overview 1
Specifications
General Instrument
Plate Capacity Standard, 8.5-inch rack
16 shallow well-plates (14 mm height)
7 deep well-plates (31 mm height)
4 deep well-plates (48 mm height)
6 vial plates for 54 × 2 ml vials
Standard, 10-inch rack
20 shallow well-plates (14 mm height)
Extended, 8.5-inch rack
64 shallow well-plates (14 mm height)
28 deep well-plates (31 mm height)
16 deep well-plates (48 mm height)
24 vial plates for 54 × 2 ml vials
Extended 10-inch rack
80 shallow well-plates
Well-plates Supported 96-well plates
96-deep-well plates
384-well plates
Well-plate with closing mats or foils
96-well plates with glass inserts and caps
Plate Storage Areas Standard
2 removable racks
Extended
5 removable racks
Housing Materials Kydex-T Plastic covering aluminum internal support plates
Gripper Materials White acetyl housing; aluminum fingers; polyurethane grip pads
Locating Plate Materials Sheet steel
Arm Mechanism Ball bearing axis with stainless steel pivot shaft; mechanical stops to
prevent continuous rotation (330°maximum)
Spill Control Interior components protected from accidental spillage by spill shield
rim located beneath rotating arm base
Ventilation/Cooling 12 volt fan located in the Well-plate Handler base

12 Agilent 1260 Infinity Sample Capacity Extension User Manual
1Overview
Robotic Arm
Physical Performance
Environmental
G2255A Well-plate Handler
Rotary Travel 330 °
Horizontal Reach 25.4 cm (10.0 in) overall reach, 14 cm (5.5 in) beyond boundary of
base
Vertical Reach Maximum 36.6 cm (14.4 in) from table, minimum 10.2 cm (4.0 in) from
table, total 26.4 cm (10.4 in) vertical travel distance
Rotary stepper motor 400 steps per motor revolutions; 0.41 mm (0.016 in) resolution per
motor step at 25.4 cm (10.0 in) radius; <5 seconds per full rotation
(330°)
Vertical Stepper Motor 400 steps per motor revolutions; 0.08 mm (0.003 in) vertical
resolution per motor step; approximately 3 seconds per complete
vertical motion (one direction)
Home Position Optical sensors for rotary and vertical arm home (zero) positions
Plate Sensor Mechanical push-button sensor
Operating Temperature 15°C to 40°C
Operating Humidity 0 to 85%, non-condensing
Storage Temperature -20°C to 65°C
Transient Over-voltages Installation Category II
Pollution Pollution Degree 2
Altitude Up to 2000M
Output 154 BTU/hr
For indoor use only

Agilent 1260 Infinity Sample Capacity Extension User Manual 13
Overview 1
G2254A Automation Interface
Physical
G2255A Well-plate Handler
G2257A including Agilent 1260 Infinity LC System
Optional Rack Extension
Operating Temperature 5°C to 55°C
Operating Humidity < 95%, at 25 – 40°C, non-condensing
Altitude Up to 2000M. For indoor use only.
Size (H ×W ×D) 49 cm ×47 cm ×72 cm
Weight 18 kg
Size (H ×W ×D)49 cm ×84 cm ×72 cm
Size (H ×W ×D)49 cm ×26 cm ×72 cm

14 Agilent 1260 Infinity Sample Capacity Extension User Manual
1Overview
Electrical
G2255A Well-plate Handler
G2254A Automation Interface
Barcodes
Power Input 100-120/200-240V AC, 50/60 Hz (autosensing), 45 watts maximum
Line Voltage 100-120/200-240V AC, autosensing
Line Frequency 50/60 Hz
Fuses For 120V operation:
One 1.5-A, 0.25 in. x 1.25 in.
(0.64 cm. x 3.2 cm.)
For 240V operation:
Two 1.0-A, 0.2 in. x 0.08 in. (5.2-mm x 20-mm).
Grounding Through the power cord
Computer Interface RS-232 serial (double shielding required)
Microprocessor Board 80C188 microprocessor-based; 1 reversible DC motor; RS-232
communications link; 2 stepper motors; 4 sensors; Auxiliary 15 pin D
Connector: 1 stepper motor, 2 - 12V outputs, 3 sensors
Line Voltage CAN DC in, 24V
Interfaces CAN to any Agilent 1260 Infinity LC module
Supported barcode types Code 39, Code 128, Codabar, 2 of 5 Interleaved
Line thickness Between 0.19 and 1 mm
Maximum width 60 mm
Protecting foil Allowed
Positioning For end-mounted barcodes, the top edge of the bars must be 9 mm or
more above the bottom edge of the plate.
Agilent Technologies

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Agilent 1260 Infinity Sample Capacity Extension
User Manual
Agilent Technologies
2
Installation
Site Preparation 16
Contents of the G2257A Shipping Kit 17
Installation 19
Adjusting the Lateral Position of the Well-plate Handler 44
Preliminary Adjustment of the Well-plate Handler Position 45
Well-plate Handler Teaching 47
Fine Adjustment of the Well-plate Handler Position 51
De-Installation of the Automation Interface 54
WARNING Switch the system power OFF before beginning these procedures.
Use only the tools listed in this manual to perform the steps described in the
instructions.
Never perform any operation on the instrument in an environment where potentially
damaging liquids or gases are present.
NOTE Do not loosen or tighten any screws, or touch parts other than those specifically
designated in the instructions. Doing so may cause misalignment and could void the
instrument warranty.
Never force any component to fit if it will not do so easily.

16 Agilent 1260 Infinity Sample Capacity Extension User Manual
2 Installation
Site Preparation
Bench Space Requirements
The Well-plate Handler should be assembled in its permanent position on a
sturdy lab bench with at least three electrical outlets nearby. It is easier to
assemble the Well-plate Handler and align it with the well-plate device if you
unplug and remove the Automation Interface from the laboratory bench first
(take note of the location of any cables connected to the device).
G2257A
G2257A with optional Rack Extension
G2257A including Agilent 1260 Infinity LC system
G2257A with optional Rack Extension & incl. Agilent 1260 Infinity LC system
No additional gases, water or exhaust are required for the G2257A.
NOTE The bench top must be sturdy enough to support the weight of the computer, the Well-plate
Handler, and the Agilent 1260 Infinity system without sagging. An uneven surface will
result in the misalignment of the Automation Interface and Well-plate Handler.
Size (H ×W ×D)49 cm ×47 cm ×72 cm
Weight 18 kg
Size (H ×W ×D)49 cm ×73 cm ×72 cm
Size (H ×W ×D)49 cm ×84 cm ×72 cm
Size (H ×W ×D)49 cm ×110 cm ×72 cm

Agilent 1260 Infinity Sample Capacity Extension User Manual 17
Installation 2
Contents of the G2257A Shipping Kit
The part numbers given in this section are for reference only. For part
numbers to use in ordering exchange, replacement of consumable items, see
“Parts List” on page 94.
G2254A Automation Interface
•Customization kit (Part No. G2255-68708) containing
Alignment assembly
Screws
Location plate
Adjustment sheet
4 spacers to adjust the Well-plate Handler height
Location pin
3 spacers to adjust the optional Rack Extension height
•Cabinet kit (Part No. G2254-68710)
•Standard Accessory kit (Part No. G2254-68705) containing
54-vial plate
CAN cable
CAN DC-out cable
Hex key
Adapter plate assembly
Country-specific power cord for the Well-plate Handler
Manual
Declaration of Conformity
G2255A Well-plate Handler
•Well-plate Handler including standard gripper set and button extension
•2 removable 8.5-inch racks (Part No. G2255-68709)
or
2 removable 10-inch racks (Part No. G2255-68710), gripper set and
screwdriver
•Well-plate Handler base plate
•RS-232 communications cable [6 ft, (1.8 m) 9-pin to 9-pin]
•13/64 inch open end wrench

18 Agilent 1260 Infinity Sample Capacity Extension User Manual
2 Installation
•Spare fuses
•Screws and washers
Barcode Reader (optional)
•Fixed barcode reader
•Hand-held barcode reader
•Adapter cable
•Hex key
•Mounting bracket
•Screws
Rack Extension (optional)
•Rack Extension base plate
•3 removable racks (8.5-inch or 10-inch)
•Screws

Agilent 1260 Infinity Sample Capacity Extension User Manual 19
Installation 2
Installation
The installation of the G2257A Sample Capacity Extension consists of the
following procedures:
•Installing the Automation Interface in the high performance autosampler,
•Setting up the Well-plate Handler,
•Calibrating the Well-plate Handler positions,
•Installing and adjusting the optional fixed barcode reader,
•Connecting the optional hand-held barcode reader.
Installing the Automation Interface
1Unpack the high performance autosampler (G1367E) or micro autosampler
(G1377A).
2Remove the front door from the autosampler (see Figure 2).
3Remove the funnel (see Figure 3).
Figure 2 Removing the front door of the Autosampler

20 Agilent 1260 Infinity Sample Capacity Extension User Manual
2 Installation
4Remove the plastic cover (see Figure 4).
5Remove any installed tray (see Figure 5).
Figure 3 Removing the funnel
Figure 4 Removing the cover