Agilent Technologies 1290 infinity User manual

111
1290 Infinity TCC User Manual
Agilent Technologies
8
Maintenance
Introduction to Maintenance and Repair 112
Cautions and Warnings 113
Overview of Maintenance 115
Cleaning the Column Compartment 116
Changing Column Identification Tags 117
Adding Heater Devices 119
Installing the capillaries 124
Correcting Leaks 131
Replacing Head Parts of Column Switching Valve 133
Replacing Valve Heads 136
Preparing the Column Compartment for Transportation 140
Replacing the Module Firmware 143
This chapter describes the maintenance tasks for the TCC. If the instrument
needs repair please contact your Agilent service representative.

112 1290 Infinity TCC User Manual
8Maintenance
Introduction to Maintenance and Repair
Introduction to Maintenance and Repair
The module is designed for easy maintenance. The most frequent
maintenances such as maintaining valve heads (if optional valve drive is
installed) or replacing low dispersion heat exchangers can be done from the
front with module in place in the system stack.
NOTE
There are no serviceable parts inside.
Do not open the module.

1290 Infinity TCC User Manual 113
Maintenance 8
Cautions and Warnings
Cautions and Warnings
WARNING
Toxic, flammable and hazardous solvents, samples and reagents
The handling of solvents, samples and reagents can hold health and safety risks.
➔When working with these substances observe appropriate safety procedures (for
example by wearing goggles, safety gloves and protective clothing) as described in
the material handling and safety data sheet supplied by the vendor, and follow good
laboratory practice.
➔The volume of substances should be reduced to the minimum required for the
analysis.
➔Do not operate the instrument in an explosive atmosphere.
CAUTION
Hot heat exchangers
The column compartment has two heat exchanger assemblies that might be hot.
➔Allow them to cool down before starting repairs.
WARNING
Electrical shock
Repair work at the module can lead to personal injuries, e.g. shock hazard, when the
cover is opened.
➔Do not remove the cover of the module.
➔Only certified persons are authorized to carry out repairs inside the module.

114 1290 Infinity TCC User Manual
8Maintenance
Cautions and Warnings
WARNING
The module is partially energized when switched off, as long as the power cord is
plugged in.
Repair work at the module can lead to personal injuries, e.g. electrical shock, when
the cover is opened and the module is connected to power.
➔Always unplug the power cable before opening the cover.
➔Do not connect the power cable to the instrument while the covers are removed.
WARNING
Personal injury or damage to the product
Agilent is not responsible for any damages caused, in whole or in part, by improper
use of the products, unauthorized alterations, adjustments or modifications to the
products, failure to comply with procedures in Agilent product user guides, or use of
the products in violation of applicable laws, rules or regulations.
➔Use your Agilent products only in the manner described in the Agilent product user
guides.
CAUTION
Safety standards for external equipment
➔If you connect external equipment to the instrument, make sure that you only use
accessory units tested and approved according to the safety standards appropriate
for the type of external equipment.
CAUTION
Sample degradation and contamination of the instrument
Metal parts in the flow path can interact with the bio-molecules in the sample leading
to sample degradation and contamination.
➔For bio-inert applications, always use dedicated bio-inert parts, which can be
identified by the bio-inert symbol or other markers described in this manual.
➔Do not mix bio-inert and non-inert modules or parts in a bio-inert system.

1290 Infinity TCC User Manual 115
Maintenance 8
Overview of Maintenance
Overview of Maintenance
The following pages describe maintenance procedures (simple repairs) that
can be done without opening the main cover.
Table 20 Maintenance Procedures
Procedure Typical Frequency Notes
“Cleaning the Column
Compartment” on page 116
If required
“Changing Column
Identification Tags” on
page 117
When column performance or new
application requires a change
“Adding Heater Devices” on
page 119
When new application requires a
change
“Installing the capillaries” on
page 124
When new application requires a
change
“Correcting Leaks” on page 131 If a leak has occurred Check for leaks
“Replacing Valve Heads” on
page 136
If the valve performance shows
indication of leakage or wear
“Preparing the Column
Compartment for
Transportation” on page 140
If the TCC shall be transported
“Replacing the Module
Firmware” on page 143
If required

116 1290 Infinity TCC User Manual
8Maintenance
Cleaning the Column Compartment
Cleaning the Column Compartment
To keep the module case clean, use a soft cloth slightly dampened with water,
or a solution of water and mild detergent.
WARNING
Liquid dripping into the electronic compartment of your module can cause shock
hazard and damage the module
➔Do not use an excessively damp cloth during cleaning.
➔Drain all solvent lines before opening any connections in the flow path.

1290 Infinity TCC User Manual 117
Maintenance 8
Changing Column Identification Tags
Changing Column Identification Tags
The column compartment is equipped with a column-identification system,
that stores column specific information. Two identification tag readers are
incorporated in the heat exchanger assemblies.
Figure 25 Column-Identification System
1The identification tag can be easily removed from the column.
2The tag needs to be placed differently, depending on whether the column is
installed at the left or right heat exchanger, see Figure 26 on page 118 and
Figure 27 on page 118. The Agilent logo should always be at front.
8dajbc"^YZci^[^XVi^dciV\
8dajbcXa^e
IV\gZVYZgh
When If column is used on the opposite heat exchanger or a tag is added to a new column.
Parts required # p/n Description
1 5062-8588 Column identification tag (1x), re-order (3/pk)

118 1290 Infinity TCC User Manual
8Maintenance
Changing Column Identification Tags
When correctly placed on the heat exchanger, the distance between tag and
tag reader is 1 – 2 mm. This is the optimum distance for proper function.
Figure 26 Column-Identification Tag for Left Heat Exchanger
Figure 27 Column-Identification Tag for Right Heat Exchanger
3For columns with small diameter, a cable tie wrap should be used to fix the
column identification tag to the column. Assure that the tie wrap does not
block the front cover.
8dajbc"^YZci^[^XVi^dciV\
8dajbc"^YZci^[^XVi^dciV\

1290 Infinity TCC User Manual 119
Maintenance 8
Adding Heater Devices
Adding Heater Devices
The additional heater devices can be arranged in the G1316C in various
locations depending on the application needs. Some examples are shown
below.
Figure 28 Arrangements of Heater and Cooling Devices

120 1290 Infinity TCC User Manual
8Maintenance
Adding Heater Devices
Figure 29 Typical positions of the low dispersion heat exchangers
The heater devices are mounted on a carrier that can be fitted to the left
and/or right heat exchangers.
NOTE
If the additional heater devices are used as shown above, the column identification system
cannot be used. If the column identification system is required, fix the heater devices in the
upper or lower locations or fix them right/left of the current location.

1290 Infinity TCC User Manual 121
Maintenance 8
Adding Heater Devices
Installing the Low Dispersion Heat Exchangers
The positions of the low dispersion heat exchangers ( Heater long-up ( 0.12
mm i.d., 1.6 μL internal volume) (G1316-80002) or Heater long-down ( 0.12
mm i.d., 1.6 μL internal volume) (G1316-80003)) depend very much on the
desired application and columns you want to use with your system. For two
long columns (> 100 mm) the carrier ( Carrier for heat exchanger
(G1316-89200)) and the heat exchangers must be installed in a middle
position. In case you are going to use columns of maximum 100 mm length it is
advised to place two carriers with one heat exchanger each at the left and the
right side of the column compartment (see Figure 29 on page 120). To use the
column tag readers the carriers must be installed either in the top or bottom
positions.
The carriers for the low-dispersion heat exchangers must be attached to the
standard built-in heat exchangers of the TCC (see Figure 30 on page 122).
Remove the protective foil from the gray thermal conductive foil of the carrier
and fasten the three screws. Mount the fitting holder assembly ( Fitting holder
assy, includes following items: Fitting fork, fitting clip, screws (pack of 4)
(G1316-68706)) on the carrier. The fitting clips hold the capillary unions from
the low dispersion heat exchangers and make plumbing of capillaries much
easier. Finally, attach the low dispersion heat exchanger. It is important to fix
them tightly so that a good thermal conductivity is achieved. The columns are
held by color-coded clips ( Column clip set, 8 colors (5042-9918)) for more
convenient installation; mark the nuts of the capillaries attached to the
columns as well with color code tags (small rings) to easily follow the flow
paths in your system.

122 1290 Infinity TCC User Manual
8Maintenance
Adding Heater Devices
Fixing the Low Dispersion Heat Exchangers
For the G1316C, additional heat exchanger devices can be installed on the
carrier Carrier for heat exchanger (G1316-89200) using 3 screws ( Screw 2.5
mm hex (0515-1052), included to part number for carrier) as shown in figure
below.
Figure 30 Installing the low dispersion heat exchangers
8Vgg^Zg[dgadl"Y^heZgh^dc
]ZVi"ZmX]Vc\Zg
Adl"Y^heZgh^dc
]ZVi"ZmX]Vc\Zg
;^ii^c\]daYZgVhhZbWan
7j^aY"^c]ZViZmX]Vc\Zg

1290 Infinity TCC User Manual 123
Maintenance 8
Adding Heater Devices
Choose Compatible Fittings
For the heater device inlet capillary choose fittings which are compatible to
your column.
Figure 31 Fitting type depends on column type
NOTE
The Very High Pressure Fitting is pressure tight up to 1200bar.
Do not overtighten the fitting at installation. Finger tighten the nut into the port until snug.
Then use the wrench and apply about 1/4 of a revolution. (Torque ~ 1.0 – 1.2 Nm)
HlV\ZadX`XdbeVi^WaZXdajbch*%+*"))*)!eVX`d[&%!l^i][ZggjaZh
;^ii^c\hXgZladc\
KZgn=^\]EgZhhjgZ;^ii^c\!gZbdkVWaZ*%+,"),(-[dghlV\ZadX`XdbeVi^WaZXdajbch

124 1290 Infinity TCC User Manual
8Maintenance
Installing the capillaries
Installing the capillaries
Installing the capillaries
The 2pos/10port valve can be used here in the same way as a 2pos/6port valve;
just follow the re-routing diagram below.
Map the ports from the 2pos/6port valve to the corresponding ports of the
2pos/10port valve according to the red arrows. For example, mount the
capillary connected to port 6 (2pos/6port) at port 2 instead.
Connect port 1 and port 8 with a 120 mm length capillary ( 0.12 mm i.d. or
0.17 mm i.d. depending on the capillary kit) (5067-4652). Plug Plastic fittings
(0100-1259) into ports 9 and 10.
&
'
(
)*
+-
&
'
(
)
*+,
.
&% EaVhi^X[^ii^c\
&'%bbaZc\i]
Parts required # p/n Description
1 5067-4646 Capillary kit
1 G4231B 2pos/6port valve
1 G4232B 2pos/10port valve
Preparations Identify the required capillaries in your capillary kits (“Capillary kit PN 5067-4646” on page 158)
NOTE
Use outmost care to avoid any void volumes caused by poor connections.

1290 Infinity TCC User Manual 125
Maintenance 8
Installing the capillaries
1Install the capillaries depending on your application:
•Dual column selection, see “Configuration for dual-column selection” on
page 127
•Sample Enrichment, see “Configuration for sample enrichment” on
page 128
•Sample Clean-up, see “Configuration for sample clean-up” on page 129
•Alternating Column Regeneration (only 2pos/10port valve), see
“Configuration for alternating column regeneration” on page 130
2Connect the capillaries connected directly to a column and fasten them
immediately with a spanner.
3Finger-tighten all remaining capillaries.
4Clip the unions into the corresponding clips of the low dispersion heat
exchangers.
5Fasten all fittings with a spanner.
6Starting from position one through six (ten, respectively), fasten the fittings
on the heat exchanger.
7Fasten all fittings on attached modules (autosampler, detector, additional
pumps). Fit all unused valve ports with a plastic plug.
8Push the valves into the rear positions.
NOTE
Use outmost care to avoid any void volumes caused by poor connections.

126 1290 Infinity TCC User Manual
8Maintenance
Installing the capillaries
9Place the capillaries that go to another module or waste into the capillary
guides to prevent squeezing them when closing the front cover.
10 Stow any excess lengths of the capillaries.
11 Perform a final leak-check.

1290 Infinity TCC User Manual 127
Maintenance 8
Installing the capillaries
Configurations and Capillary Set-up
Configuration for dual-column selection
Figure 32 Installing the capillaries for a dual-column selection set-up (column and heat
exchanger of the second position are omitted)
&
'
(
)
*
+
,
-
&
'
(
)*
+
1 150 mm length (column length up to 100 mm), 280 mm length (column length > 100 mm)
From column
Not pre-swaged on column-side!
2 200 mm length to detector
3 150 mm length (column length up to 100 mm, 280 mm length (column length > 100 mm)
From column
Not pre-swaged on column-side!
4 Column with color code clip
5 Low dispersion heat exchanger or built-in 3 µL/6 µL heat exchanger and 90 mm length capillary to
column
6 90 mm length to heat exchanger
7 Built-in 3 µL/6 µL heat exchanger and 90 mm length capillary to column
8 90 mm length to heat exchanger
Pos.1: Connection between Ports 1-6 , 4-5, 2-3 , active Column 1 = left
Pos. 2: Connection between Ports 1-2, 3-4, 5-6 active Column 2 = right
Example shows setup with flow directed 1290 typical from bottom to top.
Flow direction from top to bottom needs switch of connected capillaries at ports 5 and 2. Also
column inlet connections needed to be switched with outlet connections. Port 4 to 3 and 6 to 1.)

128 1290 Infinity TCC User Manual
8Maintenance
Installing the capillaries
Configuration for sample enrichment
Figure 33 Installing the capillaries for a sample enrichment set-up
&
'
(
)
*
+
,
-
&%
.
&&
&
'
(
)*
+
1Analytical column with color code clip
2 280 mm length (column length > 100 mm) from analytical column to detector
Not pre-swaged on column-side!
3 340 mm length from autosampler and loading pump
4Towaste
5 150 mm length (column length up to 100 mm), 280 mm length (column length > 100 mm)
From column
Not pre-swaged on column-side!
6Enrichment column with color code clip
7 Low dispersion heat exchanger or build-in 3 µL/6 µL heat exchanger and 90 mm length
capillary to column
8 90 mm length to heat exchanger
9 700 mm length (0.17 mm ID) from analytical pump
10 90 mm length to heat exchanger
11 Low dispersion heat exchanger or build-in 3 µL/6 µL heat exchanger and 90 mm length
capillary to column
Pos.1: Connection between ports 1-6 , 4-5, 2-3 , active column 1 = left (enrichment column)
Pos. 2: Connection between ports 1-2, 3-4, 5-6 active column 2 = right (analytical colum)

1290 Infinity TCC User Manual 129
Maintenance 8
Installing the capillaries
Configuration for sample clean-up
Figure 34 Installing the capillaries for a sample clean-up set-up
&
'
(
)
*
+
,
-
.
&%
&&
&
'
(
)*
+
1Analytical column with color code clip
2 280 mm length (column length > 100 mm) from analytical column to detector
Not pre-swaged on column-side!
3 340 mm length from autosampler and loading pump
4 150 mm length (column length up to 100 mm), 280 mm length (column length > 100 mm)
From column
Not pre-swaged on column-side!
5Pre-column with color code clip
6 Low dispersion heat exchanger or build-in 3 µL/6 µL heat exchanger and 90 mm length
capillary to column
7 90 mm length to heat exchanger
8 700 mm length (0.17 mm ID) from analytical pump
9Towaste
10 90 mm length to heat exchanger
11 Low dispersion heat exchanger or build-in 3 µL/6 µL heat exchanger and 90 mm length
capillary to column
Pos.1: Connection between ports 1-6 , 4-5, 2-3 , active column 1 = left (Pre-column)
Pos. 2: Connection between Ports 1-2, 3-4, 5-6 active column 2 = right (analytical column)

130 1290 Infinity TCC User Manual
8Maintenance
Installing the capillaries
Configuration for alternating column regeneration
Figure 35 Installing the capillaries for alternating column regeneration (column and heat
exchanger of the second position are omitted)
&
'
(
)
*
+
,
.
-
&%
&&
&
'
(
)
*+,
-
.
&%
1 Valve-Valve connector, 120 mm length
2 150 mm length (column length up to 100 mm), 280 mm length (column length > 100 mm)
From column
Not pre-swaged on column-side!
3 200 mm length to detector
4 150 mm length (column length up to 100 mm), 280 mm length (column length > 100 mm)
From column
Not pre-swaged on column-side!
5 Column with color code clip
6 Low dispersion heat exchanger or build-in 3µL/6µL heat exchanger and 90 mm length
capillary to column
7 90 mm length to heat exchanger
8Towaste
9 From autosampler
10 700 mm length (0.17 mm ID) from regeneration pump
11 90 mm length to heat exchanger
Pos.1: Connection between ports 1-10 , 2-3, 4-5, 6-7, 8-9 active column 1 = left /
regemerating column = right
Pos. 2: Connection between ports 1-2, 3-4, 5-6, 7-8, 9-10 active column 2 = right /
regenerating column = left