Agilent Technologies 2200 TapeStation User manual

Agilent Technologies
Agilent 2200 TapeStation
User Manual

Agilent 2200 TapeStation - User Manual
Notices
© Agilent Technologies, Inc. 2011-2013,
2014
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tronic storage and retrieval or translation
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Manual Part Number
G2964-90003
Edition
10/2014
Printed in Germany
Agilent Technologies
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76337 Waldbronn
This product may be used as a com-
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Agilent 2200 TapeStation - User Manual 3
Contents
Contents
1 Introduction to the 2200 TapeStation System 5
Overview of the System 6
2 Specifications 11
Technical Specifications 12
ScreenTape Specifications 14
3 Installing the System 21
Unpacking the System 22
Contents of the ScreenTape System 24
Installing the System 27
4 Using the 2200 TapeStation System 31
Intended Use of the 2200 TapeStation System 33
Performance Limitations of Use 34
Additional Components Required by the User 35
Operating Procedure 36
How to prepare your samples 48
Good Measurement Practices for Analysing DNA on the Agilent 2200
Ta p e S ta t i o n 50
DNA Sample Preparation 51
RNA Sample Preparation 57
Protein Sample Preparation 63
5 Maintenance 67
General Information 68
Changing the Needle 69
Preventative Maintenance Interval 72

4 Agilent 2200 TapeStation - User Manual
Contents
6 Appendix 73
Limited Use Label License 74
Sound Emission 75
The Waste Electrical and Electronic Equipment Directive 76
Technical Service 77

5
Agilent 2200 TapeStation - User Manual
Agilent Technologies
1
Introduction to the 2200 TapeStation
System
Overview of the System 6
ScreenTape Architecture 7
Agilent 2200 TapeStation Components 8
This chapter gives an introduction to the system

6 Agilent 2200 TapeStation - User Manual
1Introduction to the 2200 TapeStation System
Overview of the System
Overview of the System
The Agilent 2200 TapeStation system is a revolutionary automated
platform for simpler, faster and more reliable electrophoresis. It is made
up of three elements: a consumable tape (ScreenTape), an instrument (the
2200 TapeStation) and an analysis software. The system is very
straightforward to use, simply place the sample tubes and ScreenTape in
to the 2200 TapeStation and let it load, separate, image, analyse and
present the results.
This User Manual guides the operation of ScreenTape, the 2200
TapeStation and software for the analysis of DNA, RNA and protein
samples. The contents of the ScreenTape System are detailed below.
Information pertaining to the 2200 TapeStation can be found in:
•2200 TapeStation Technical Specification
•2200 TapeStation Components
•Installing the 2200 TapeStation
Place ScreenTape and some tips in the 2200 TapeStation.
Place your samples in the 2200 TapeStation and click Start on the
controller software.
View your analysed results in around 1 min per sample.

Agilent 2200 TapeStation - User Manual 7
Introduction to the 2200 TapeStation System 1
Overview of the System
Information pertaining to sample and ScreenTape requirements can be
found in:
•ScreenTape Architecture
•Operating Procedure
ScreenTape Architecture
Figure 1 Example: P200 ScreenTape
Barcode: The unique barcode tracks lane usage within individual ScreenTape and
allows traceability of results.
Buffer chamber: The buffer chamber is located at the top of the channel and contains
optimised buffers for the effective separation of nucleic acid fragments or
proteins.
Electrodes: The integrated electrodes apply a current across the ScreenTape and
eliminate the need for any additional electrophoresis equipment.
Gel: The gel contained within ScreenTape has been developed specifically to
resolve nucleic acids or proteins.
ScreenTape product
details:
The information is unique to each consumable item. This includes:
ScreenTape type, product expiry date and a unique serial number.

8 Agilent 2200 TapeStation - User Manual
1Introduction to the 2200 TapeStation System
Overview of the System
Agilent 2200 TapeStation Components
Lid: The 2200 TapeStation lid must be closed each time the instrument
controller software is initialized, and whilst the instrument is in operation.
LED: The LED will illuminate once the instrument is on. When the LED is
flashing slowly, the instrument is in use and the lid should not be opened,
rapid flashing indicates that the TapeStation requires attention.
Sample Block: There are 2 sample blocks provided that can either hold 0.2 mL sample
tube strips or a 96-well plate.
Tip Holder: The tip holder can accommodate up to 16 TapeStation loading tips at any
one time.
ScreenTape: The tape must be placed into the holder with the barcode towards the
front of the instrument, facing towards the right.
USB Socket: The USB connector is inserted into the USB socket to link the laptop to the
2200 TapeStation.
Power-cable socket: The power cable must be connected to the 2200 TapeStation and the
relevant mains electricity outlet.

Agilent 2200 TapeStation - User Manual 9
Introduction to the 2200 TapeStation System 1
Overview of the System
Figure 2 2200 TapeStation (front view)
Figure 3 2200 TapeStation (back view)
Lid
ScreenTape
Tip holder
LED
Sample block
USB socket
On/off switch
Power-cable socket

10 Agilent 2200 TapeStation - User Manual
1Introduction to the 2200 TapeStation System
Overview of the System

11
Agilent 2200 TapeStation - User Manual
Agilent Technologies
2
Specifications
Technical Specifications 12
ScreenTape Specifications 14
This chapter provides information on specifications.

12 Agilent 2200 TapeStation - User Manual
2Specifications
Technical Specifications
Technical Specifications
2200 TapeStation
Input voltage: 12 V DC
Power consumption: 40 W (VA)
Current: 3 A
Interface: USB cable (PC comms.)
Instrument Housing: UL94/VO rated flame retardant cast polyurethane
Dimensions: 400x 310x 310 mm
Weight: 12.5 kg
Power Supply
Input voltage: 100 – 240 V AC
Input frequency: 50 – 60 Hz
Phase: 1
Current: 0.45 – 1.1 A
Environmental condition
Optimal operating
temperature
20 °C (68 °F).
Instrument operating
temperature1
1Instrument operating temperature may be higher than ambient lab temperature, especially after
prolonged use.
12 – 37 °C (54 – 99 °F) for D1000 ScreenTape
17 – 37 °C (63 – 99 °F) for High Sensitivity D1000 ScreenTape
15 – 30 °C (59 – 86 °F) for Genomic DNA ScreenTape
14 – 30 °C (57 – 86 °F) for RNA and High Sensitivity RNA ScreenTape
10 – 33 °C (50 – 91 °F) for P200 ScreenTape

Agilent 2200 TapeStation - User Manual 13
Specifications 2
Technical Specifications
NOTE
If the instrument is out of the recommended temperature range for the ScreenTape inserted
the following error message will appear in the controller software:
•If the quoted current temperature is above the specified range, please move the
system out of direct sunlight and away from any windows. Check that any air
conditioning is functioning.
•If the quoted current temperature is below the specified range please allow the
instrument to equilibrate to the ambient temperature, and avoid using in a cooled
area.

14 Agilent 2200 TapeStation - User Manual
2Specifications
ScreenTape Specifications
ScreenTape Specifications
Specification (D1000 ScreenTape Assay)
Analytical Specifications D1000 ScreenTape and Reagents
Sizing Range 35 – 1000 bp
Typical Resolution 35 – 300 bp: 15 %
300 – 1000 bp: 10 %
Sensitivity1
1signal-to-noise >3 (single peak)
0.1 ng/µL
Sizing Precision 5 % CV
Sizing Accuracy2
2Sizing Accuracy for software ladder: ±20%
±10 %
Quantitative Precision 0.1 – 1 ng/µL: 15 % CV
1 – 50 ng/µL: 10 % CV
Quantitative Accuracy3
3Measured against 2100 Bioanalyzer
±20 %
Quantitative Range 0.1 – 50 ng/µL
Maximum sample buffer strength 20 mM KCl,
60 mM Phosphate Buffer,
60 mM Guanidine-HCl,
240 mM NaCl,
60 mM Acetate
Physical Specifications
Analysis Time 16 samples: <20 min
96 samples: ≈100 min
Samples per consumable 16
Sample volume required 1 µL
Kit stability 4 months
Kit size 112 samples

Agilent 2200 TapeStation - User Manual 15
Specifications 2
ScreenTape Specifications
Specification (High Sensitivity D1000 ScreenTape Assay)
Analytical Specifications High Sensitivity D1000 ScreenTape and Reagents
Sizing Range 35 – 1000 bp
Typical Resolution 35 – 300 bp: 15 %
300 – 1000 bp: 10 %
Sensitivity1
1signal-to-noise >3 (single peak)
5pg/µL
Sizing Precision 5 % CV
Sizing Accuracy2
2Sizing Accuracy for software ladder: ±20%
±10 %
Quantitative Precision 15 % CV
Quantitative Accuracy3
3Measured against 2100 Bioanalyzer
±20 %
Quantitative Range 10 – 1000 pg/µL
Maximum sample buffer strength 7 mM KCl,
20 mM Phosphate Buffer,
20 mM Guanidine-HCl,
80 mM NaCl,
20 mM Acetate
Physical Specifications
Analysis Time 16 samples: <20 min
96 samples: ≈100 min
Samples per consumable 16
Sample volume required 2 µL
Kit stability 4 months
Kit size 112 samples

16 Agilent 2200 TapeStation - User Manual
2Specifications
ScreenTape Specifications
Specification (Genomic DNA ScreenTape Assay)
Analytical Specification Genomic DNA ScreenTape and Reagents
Sizing Range 200 bp to > 60000 bp
Sensitivity 0.5 ng/µL
Sizing Precision1
1Determined using the Genomic DNA Ladder as sample
200 – 15000 bp: 15 % CV
Sizing Accuracy1200 – 15000 bp: ±15 %
Quantitative Precision2
2Average result from various genomic DNA sample types
15 % CV
Quantitative Accuracy3±20 %
Linear Concentration Range 10 – 100 ng/µL
DIN functional range 5 – 300 ng/µL
Physical Specification
Analysis Time 16 samples: < 25 min
96 samples: < 150 min
Samples per consumable 16
Sample Volume Required 1 µL
Shelf Life 4 months
Box/Kit size 112 samples/box

Agilent 2200 TapeStation - User Manual 17
Specifications 2
ScreenTape Specifications
Specification (RNA ScreenTape Assay)
Analytical Specification RNA ScreenTape and Reagents
Quality Score RINe
Quantitative Range 25 – 500 ng/µL
Quantitative Precision (%CV)15%
Quantitative Accuracy 20 %
Sizing Range 100 – 6000 nt
Sensitivity25ng/µL
RINefunctional range 25 – 500 ng/µL
Analysis Type Eukaryotic or Prokaryotic Total RNA QC
Maximum sample buffer strength 200 mM Tris,
20 mM EDTA, or
50 mM NaCl
Physical Specifications
Analysis Time 16 samples < 16 min
96 samples < 100 min
Samples per consumable 16
Sample volume required (µL) 1
Kit Stability 4 months
Kit Size 112 samples
For total RNA samples
1Within a ScreenTape 2Signal/noise >3 in water and TE

18 Agilent 2200 TapeStation - User Manual
2Specifications
ScreenTape Specifications
Specification (High Sensitivity RNA ScreenTape Assay)
Analytical Specification RNA ScreenTape and Reagents
Quality Score RINe
Quantitative Range 500 – 10000 pg/µL
Quantitative Precision (%CV)110 %
Quantitative Accuracy 30 %
Sizing Range 100 – 6000 nt
Sensitivity2100 pg/µL
RINefunctional range 1000 – 25000 pg/µL
Analysis Type Eukaryotic or Prokaryotic Total RNA QC
Maximum sample buffer strength 10 mM Tris
1mMEDTA
Physical Specifications
Analysis Time 16 samples < 30 min
96 samples < 180 min
Samples per consumable 16
Sample volume required (µL) 2
Kit Stability 4 months
Kit Size 112 samples
For total RNA samples
1Within a ScreenTape 2Signal/noise >3 in water and TE

Agilent 2200 TapeStation - User Manual 19
Specifications 2
ScreenTape Specifications
Specification (P200 ScreenTape Assay)
Analytical Specification P200 ScreenTape and Reagents
Sizing range 10 – 200 kDa
Resolution1
1determined using P200 Lader as sample
15 %
Typical Sizing Accuracy ±10 % (CAII, Lysozyme, beta lactoglobulin)
Sizing Precision 3 % CV
Quantitative Range/precision 100 – 1000 ng/µL for IgG; 15 % CV
Qualitative Range 5 – 5000 ng/µL BSA, Lysozyme; 12.5 – 5000 ng/µL IgG
Sensitivity2
2signal :noise ratio > 3
5 ng/µL Lysozyme; 12.5 ng/µL IgG
Physical Specification
Sample volume needed 2 µL
Analysis Time 16 samples: <15 min
Samples/consumable 16
Kit Size 112 Samples
Kit Stability 4 months

20 Agilent 2200 TapeStation - User Manual
2Specifications
ScreenTape Specifications