Agilent Technologies 2200 TapeStation User manual

Agilent Technologies
Agilent 2200 TapeStation
User Manual

Agilent 2200 TapeStation - User Manual
Notices
© Agilent Technologies, Inc. 2011-2013,
2014
No part of this manual may be reproduced
in any form or by any means (including elec-
tronic storage and retrieval or translation
into a foreign language) without prior agree-
ment and written consent from Agilent
Technologies, Inc. as governed by United
States and international copyright laws.
Manual Part Number
G2964-90003
Edition
10/2014
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
This product may be used as a com-
ponent of an in vitro diagnostic sys-
tem if the system is registered with
the appropriate authorities and com-
plies with the relevant regulations.
Otherwise, it is intended only for gen-
eral laboratory use.
Warranty
The material contained in this docu-
ment is provided “as is,” and is sub-
ject to being changed, without notice,
in future editions. Further, to the max-
imum extent permitted by applicable
law, Agilent disclaims all warranties,
either express or implied, with regard
to this manual and any information
contained herein, including but not
limited to the implied warranties of
merchantability and fitness for a par-
ticular purpose. Agilent shall not be
liable for errors or for incidental or
consequential damages in connection
with the furnishing, use, or perfor-
mance of this document or of any
information contained herein. Should
Agilent and the user have a separate
written agreement with warranty
terms covering the material in this
document that conflict with these
terms, the warranty terms in the sep-
arate agreement shall control.
Technology Licenses
The hardware and/or software described in
this document are furnished under a license
and may be used or copied only in accor-
dance with the terms of such license.
Restricted Rights Legend
If software is for use in the performance of a
U.S. Government prime contract or subcon-
tract, Software is delivered and licensed as
“Commercial computer software” as
defined in DFAR 252.227-7014 (June 1995),
or as a “commercial item” as defined in FAR
2.101(a) or as “Restricted computer soft-
ware” as defined in FAR 52.227-19 (June
1987) or any equivalent agency regulation
or contract clause. Use, duplication or dis-
closure of Software is subject to Agilent
Technologies’ standard commercial license
terms, and non-DOD Departments and
Agencies of the U.S. Government will
receive no greater than Restricted Rights as
defined in FAR 52.227-19(c)(1-2) (June
1987). U.S. Government users will receive
no greater than Limited Rights as defined in
FAR 52.227-14 (June 1987) or DFAR
252.227-7015 (b)(2) (November 1995), as
applicable in any technical data.
Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated condi-
tions are fully understood and
met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
result in personal injury or
death. Do not proceed beyond a
WARNING notice until the indi-
cated conditions are fully under-
stood and met.

Agilent 2200 TapeStation - User Manual 3
Contents
Contents
1 Introduction to the 2200 TapeStation System 5
Overview of the System 6
2 Specifications 11
Technical Specifications 12
ScreenTape Specifications 14
3 Installing the System 21
Unpacking the System 22
Contents of the ScreenTape System 24
Installing the System 27
4 Using the 2200 TapeStation System 31
Intended Use of the 2200 TapeStation System 33
Performance Limitations of Use 34
Additional Components Required by the User 35
Operating Procedure 36
How to prepare your samples 48
Good Measurement Practices for Analysing DNA on the Agilent 2200
Ta p e S ta t i o n 50
DNA Sample Preparation 51
RNA Sample Preparation 57
Protein Sample Preparation 63
5 Maintenance 67
General Information 68
Changing the Needle 69
Preventative Maintenance Interval 72

4 Agilent 2200 TapeStation - User Manual
Contents
6 Appendix 73
Limited Use Label License 74
Sound Emission 75
The Waste Electrical and Electronic Equipment Directive 76
Technical Service 77

5
Agilent 2200 TapeStation - User Manual
Agilent Technologies
1
Introduction to the 2200 TapeStation
System
Overview of the System 6
ScreenTape Architecture 7
Agilent 2200 TapeStation Components 8
This chapter gives an introduction to the system

6 Agilent 2200 TapeStation - User Manual
1Introduction to the 2200 TapeStation System
Overview of the System
Overview of the System
The Agilent 2200 TapeStation system is a revolutionary automated
platform for simpler, faster and more reliable electrophoresis. It is made
up of three elements: a consumable tape (ScreenTape), an instrument (the
2200 TapeStation) and an analysis software. The system is very
straightforward to use, simply place the sample tubes and ScreenTape in
to the 2200 TapeStation and let it load, separate, image, analyse and
present the results.
This User Manual guides the operation of ScreenTape, the 2200
TapeStation and software for the analysis of DNA, RNA and protein
samples. The contents of the ScreenTape System are detailed below.
Information pertaining to the 2200 TapeStation can be found in:
•2200 TapeStation Technical Specification
•2200 TapeStation Components
•Installing the 2200 TapeStation
Place ScreenTape and some tips in the 2200 TapeStation.
Place your samples in the 2200 TapeStation and click Start on the
controller software.
View your analysed results in around 1 min per sample.

Agilent 2200 TapeStation - User Manual 7
Introduction to the 2200 TapeStation System 1
Overview of the System
Information pertaining to sample and ScreenTape requirements can be
found in:
•ScreenTape Architecture
•Operating Procedure
ScreenTape Architecture
Figure 1 Example: P200 ScreenTape
Barcode: The unique barcode tracks lane usage within individual ScreenTape and
allows traceability of results.
Buffer chamber: The buffer chamber is located at the top of the channel and contains
optimised buffers for the effective separation of nucleic acid fragments or
proteins.
Electrodes: The integrated electrodes apply a current across the ScreenTape and
eliminate the need for any additional electrophoresis equipment.
Gel: The gel contained within ScreenTape has been developed specifically to
resolve nucleic acids or proteins.
ScreenTape product
details:
The information is unique to each consumable item. This includes:
ScreenTape type, product expiry date and a unique serial number.

8 Agilent 2200 TapeStation - User Manual
1Introduction to the 2200 TapeStation System
Overview of the System
Agilent 2200 TapeStation Components
Lid: The 2200 TapeStation lid must be closed each time the instrument
controller software is initialized, and whilst the instrument is in operation.
LED: The LED will illuminate once the instrument is on. When the LED is
flashing slowly, the instrument is in use and the lid should not be opened,
rapid flashing indicates that the TapeStation requires attention.
Sample Block: There are 2 sample blocks provided that can either hold 0.2 mL sample
tube strips or a 96-well plate.
Tip Holder: The tip holder can accommodate up to 16 TapeStation loading tips at any
one time.
ScreenTape: The tape must be placed into the holder with the barcode towards the
front of the instrument, facing towards the right.
USB Socket: The USB connector is inserted into the USB socket to link the laptop to the
2200 TapeStation.
Power-cable socket: The power cable must be connected to the 2200 TapeStation and the
relevant mains electricity outlet.

Agilent 2200 TapeStation - User Manual 9
Introduction to the 2200 TapeStation System 1
Overview of the System
Figure 2 2200 TapeStation (front view)
Figure 3 2200 TapeStation (back view)
Lid
ScreenTape
Tip holder
LED
Sample block
USB socket
On/off switch
Power-cable socket

10 Agilent 2200 TapeStation - User Manual
1Introduction to the 2200 TapeStation System
Overview of the System