Agilent Technologies 1290 Infinity Flexible Cube User manual

Agilent Technologies
Agilent 1290 Infinity
Flexible Cube
User Manual

1290 Infinity Flexible Cube User Manual
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Manual Part Number
G4227-90000 Rev. C
Edition
10/2018
Printed in Germany
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1290 Infinity Flexible Cube User Manual 3
In This Guide...
In This Guide...
This manual covers the Agilent 1290 Infinity Flexible Cube (G4227A).
1Introduction
This chapter gives an introduction to the module, instrument overview and
internal connectors.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3 Installing the Module
This chapter gives information about the preferred stack setup for your
system and the installation of your Flexible Cube.
4 LAN Configuration
This chapter provides information on connecting the module to the Agilent
Data System.
5 Using the Module
This chapter provides information on how to set up the Flexible Cube for an
analysis and explains the basic settings.
6 Optimizing Lab Performance with Flexible Cube
This chapter provides information on how to optimize the Autosampler and
the Flexible Cube for a minimum carryover and fastest cycle times.
7 Troubleshooting and Diagnostics
This chapter gives an overview about the troubleshooting and diagnostic
features and the different user interfaces.

4 1290 Infinity Flexible Cube User Manual
In This Guide...
8 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.
9 Test Functions
This chapter describes the tests for the module.
10 Maintenance and Repair
This chapter describes the maintenance of the module.
11 Parts and Materials for Maintenance
This chapter provides information on parts and material required for the
module.
12 Identifying Cables
This chapter provides information on cables used with the 1290 series of
HPLC modules.
13 Hardware Information
This chapter describes the module in more detail on hardware and
electronics.
14 Appendix
This chapter provides addition information on safety, legal and web.

1290 Infinity Flexible Cube User Manual 5
Contents
Contents
1Introduction 9
Features 10
Overview of the Module 11
Flexible Cube Technical Capability 12
Valve solution / Valve technique 19
Bio-inert Materials 26
2 Site Requirements and Specifications 29
Site Requirements 30
Physical Specifications 33
Performance Specifications 34
3 Installing the Module 39
Unpacking the Module 40
Optimizing the Stack Configuration 42
Installing the Module 49
Flow Connections to the Module for Lowest Carryover 51
Installing the Valve Heads 53
4 LAN Configuration 59
LAN-Configuration 60
5 Using the Module 61
Instrument Configuration 62
Setting up the Flexible Cube with Agilent ChemStation 64
Solvent Information 80
Algae Growth in HPLC Systems 85

6 1290 Infinity Flexible Cube User Manual
Contents
6 Optimizing Lab Performance with Flexible Cube 87
Preparing the Autosampler and Flexible Cube 88
How to Achieve Lowest Carryover 89
How to Achieve the Highest Throughput Using the Flexible Cube 97
How to Achieve Higher Injection Volumes 98
How to Achieve High Throughput 99
7 Troubleshooting and Diagnostics 101
Overview of the Module’s Indicators and Test Functions 102
Status Indicators 103
User Interfaces 105
Agilent Lab Advisor Software 106
8 Error Information 107
What Are Error Messages 108
General Error Messages 109
Module Error Messages 116
9 Test Functions 121
Introduction 122
Pressure Test 123
10 Maintenance and Repair 125
Introduction to Maintenance 126
Warnings and Cautions 127
Overview of Maintenance 128
Cleaning the Module 129
Exchange Flush Pump Inlet Valve 130
Exchange Flush Pump Outlet Valve 132
Exchange Valve Rotor Seal 134
Replacing Parts of the Valve Head 137
Replacing Valve Heads 139
Installing the Capillaries 143
Replacing Module Firmware 150

1290 Infinity Flexible Cube User Manual 7
Contents
11 Parts and Materials for Maintenance 151
Parts overview 152
Capillaries 153
Accessory Kits 154
Valve Options Overview 156
2 Position/6 Port Valve Head 800 bar, 5067-4282 158
2 Position/6 Port Valve Head 1300 bar, 5067-4241 159
2 Position/6 Port Valve Head 600 bar (Bio-inert), 5067-4148 160
2 Position/10 Port Micro Valve Head 600 bar, 5067-4144 161
2 Position/10 Port Valve Head 1300 bar, 5067-4240 162
8 Position/9 Port Valve Head 600 bar, 5067-4107 163
4-Column Selector Valve Head 600 bar (Bio-inert), 5067-4134 164
6-Column Selector Valve Head 800 bar, 5067-4284 165
6-Column Selector Valve Head 1300 bar, 5067-4273 166
12 Position/13 Port Valve Head 210 bar (Bio-inert), 5067-4159 167
12 Identifying Cables 169
Cable Overview 170
Analog Cables 172
Remote Cables 174
BCD Cables 177
CAN/LAN Cables 179
External Contact Cable 180
Agilent Module to PC 181
Agilent 1200 Module to Printer 182
13 Hardware Information 183
Firmware Description 184
Electrical Connections 187
Interfaces 189
Setting the 8-bit Configuration Switch (without On-board) LAN 196
Instrument Layout 200
Early Maintenance Feedback 201

8 1290 Infinity Flexible Cube User Manual
Contents
14 Appendix 203
General Safety Information 204
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002/96/EC) 207
Radio Interference 208
Sound Emission 209
Agilent Technologies on Internet 210

9
1290 Infinity Flexible Cube User Manual
Agilent Technologies
1
Introduction
Features 10
Overview of the Module 11
Flexible Cube Technical Capability 12
Valve solution / Valve technique 19
Dual and Multi Column Selection 19
Sample Enrichment and Sample Cleanup 21
Alternating Column Regeneration 24
Solvent Selection 25
Bio-inert Materials 26
This chapter gives an introduction to the module, instrument overview and
internal connectors.

10 1290 Infinity Flexible Cube User Manual
1 Introduction
Features
Features
The Agilent 1290 Infinity Flexible Cube introduces an unprecedented
flexibility to the Agilent 1290 Infinity System. It includes options to reduce
carryover, and optionally you can add one or two valve heads to be able to use
the G4226A HiP Autosampler as a multipurpose valve box.
The G4227A Flexible Cube is capable of reducing carryover to a minimum and
maintains that level between the maintenance intervals of the Autosampler.
For specifications, see “Performance Specifications” on page 34.
NOTE
This 1290 Infinity Flexible Cube has been introduced as part of the Agilent 1290 Infinity
Liquid Chromatograph.