Agilent Technologies CrossLab RapidFire 400 User manual

RapidFire 400 System
Quick Start Guide
Where to find more information 2
User guides 2
Web Site 2
Getting Started 3
System Preparation 3
Run the RapidFire 400 System in Plates Mode 9
Run the RapidFire System in Sequences mode 11
RapidFire 400 System Flow Path 15
State 1: Aspirate 15
State 2: Wash/Load 16
State 3: Extra Wash 17
State 4: Elute 18
State 5: Re-equilibrate 19
Flush the sipper tube 20
Physical colors of the tubing 21
Valve positions 21

2 RapidFire 400 System Quick Start Guide
Where to find more information
User guides
You can access these guides from the RapidFire Resource App which you can
start from the RapidFire Resources icon.
User Guide This guide describes how to prepare, maintain, and run the
RapidFire 400 System.
Data Analysis Guide This guide describes how to use the RapidFire Integrator
software to prepare and analyze data.
Safety Guide This guides contains the safety instructions for operating the
RapidFire 400 System.
Web Site
Go to www.agilent.com for more information about the RapidFire System.

RapidFire 400 System Quick Start Guide 3
Getting Started
Refer to the RapidFire 400 System User Guide for detailed instructions.
System Preparation
System preparation
Step Details
1Prepare solvents Pull out the solvent drawer and make sure you have enough solvent.
2Check waste capacity Check that the waste containers have enough room to accommodate
the waste that will be generated from the runs that are planned.
3Start the vacuum system Open up the vacuum valve or switch on your standalone vacuum
pump.

4 RapidFire 400 System Quick Start Guide
4Start the software aDouble-click the RapidFire Control Panel desktop icon.
bClick File > Choose CFGs.
cSelect the configuration folder under C:\Agilent\RapidFire.
dClick Start in the Control Panel dialog box.
System preparation
Step Details

RapidFire 400 System Quick Start Guide 5
5Verify network settings aDouble-click the RapidFire UI desktop icon.
bClick System Tools > Network Settings.
cVerify that the IP addresses for the RapidFire computer and the
mass spectrometer computer are correct.
dClick Connect in the main window.
6Home the cartridge changer
and insert your cartridges
Click System Tools > Cartridge Changer. Refer to the User Guide for
more details.
System preparation
Step Details

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7Home the stages aClick System Tools > Sipper Configuration.
bSelect the plate type you are using from the Plate to Configure list.
System preparation
Step Details

RapidFire 400 System Quick Start Guide 7
8Home the valves aClick System Tools > Valve Tuner to open the Valve Configuration
dialog box.
bClick Find to home a valve.
Refer to the User Guide for details.
9Prepare the peristaltic pump aCheck that Pump 4 is turned on and rotating clockwise.
bEnsure that the vacuum is at least -70 kPa.
System preparation
Step Details

8 RapidFire 400 System Quick Start Guide
10 Prepare the quaternary pumps aOpen the purge valves.
bTurn on the pump flow.
cTurn off the pump flow once the lines are purged.
dClose the purge valves.
11 Flush the sipper tube Under Sipper Settings, click Flush Now.
12 Prepare the MassHunter Data
Acquisition program
aIf you have two controlling computers (one for the mass spec (MS)
and one for RapidFire), on the MS computer, double-click on the
RapidFire Communicator desktop icon.
bStart the Data Acquisition program.
cTurn on the detector.
dCalibrate the mass spec.
eCreate or edit a method settings that are appropriate for your
analysis.
fSave the method.
System preparation
Step Details

RapidFire 400 System Quick Start Guide 9
Run the RapidFire 400 System in Plates Mode
Running the RapidFire 400 System in Plates Mode
Step Details
1Prepare the plates. Thaw sample plates and spin on a centrifuge at 3000 rpm for five minutes.
2Load the plates. Manually place a plate on the stage or load the stacks to use the plate
handler.
3Prepare the MassHunter
Data Acquisition program.
aOpen or create a method and set parameters for your analysis.
bSet up a Manual Run. Set injection volume to As Instrument.
cRun the sample.

10 RapidFire 400 System Quick Start Guide
4Set the RapidFire system
run parameters.
aSelect the Plates option.
bSet the parameters for your analysis in the RapidFire software main
window.
cSave the settings as a method.
5Start the run in Plates
mode.
Click the play button.
Running the RapidFire 400 System in Plates Mode
Step Details

RapidFire 400 System Quick Start Guide 11
Run the RapidFire System in Sequences mode
The following diagram illustrates the terminology used to define a batch of plates
run in Sequences mode.
To run in Sequence mode:
Step Details
1Prepare the plates. Thaw sample plates and spin in a centrifuge at 3000 rpm for five minutes.
2Load the plates. Manually place a plate on the stage or load the stacks to use the plate
handler.
3Prepare the MassHunter
Data Acquisition program.
Make sure the MassHunter Data Acquisition program is prepared as
described in “System Preparation” on page 3.

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4Create a method file. aSelect Sequences.
bSet the parameters in the RapidFire software.
cClick File > Save RF method as. (file type .rfcfg)
To run in Sequence mode:
Step Details

RapidFire 400 System Quick Start Guide 13
5Create or edit a plate map
that has your sequences.
aClick Batch Configuration Tools > Plate Map Editor.
bCreate or edit one or more sequences.
cClick File > Save as (file type .rfmap).
To run in Sequence mode:
Step Details

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6Create or edit a batch. aClick Batch Configuration Tools > Batch Editor.
bCreate or edit a batch.
cSelect a RapidFire method, cartridge, and a MS method.
dClick File > Save as (file type .rfbat).
7Set the run parameters. Select your plate configuration and miscellaneous run settings.
8Start the run. Click File > Load RF batch (file type .rfbat) and start the run.
To run in Sequence mode:
Step Details

RapidFire 400 System Quick Start Guide 15
RapidFire 400 System Flow Path
State 1: Aspirate

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State 2: Wash/Load

RapidFire 400 System Quick Start Guide 17
State 3: Extra Wash

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State 4: Elute

RapidFire 400 System Quick Start Guide 19
State 5: Re-equilibrate

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Flush the sipper tube