Analytik Jena Biometra TOne Manual

Setup Instructions
Biometra TOne
High-performance thermal cycler for
DNA amplification by PCR

Biometra GmbH (Teil der Analytik Jena GmbH)
Rudolf-Wissell-Str. 30
37079 Göttingen Germany
Phone + 49 551 50 68 6-0
Fax + 49 551 50 68 6-66
Email [email protected]m
Biometra GmbH
Rudolf-Wissell-Str. 30
37079 Göttingen Germany
Phone + 49 551 50 68 6 -10, -14 or -16
Fax + 49 551 50 68 6-66
Email [e-mail]
http://www.analytik-jena.com
These setup instructions are valid from software version ME 1.70 –RE 1.46
For a proper and safe use of this product follow the instructions.
Keep the setup instruction for future reference.
Microsoft and Windows are registered trademarks of Microsoft Corp.
The identification with ® or TM is omitted in this operating manual.
34-3000-003-23
01.21
Analytik Jena GmbH
© Copyright 2021 Analytik Jena GmbH
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Biometra TOne Contents
Version 01.21 3
Contents
1Basic information ....................................................................................................... 4
1.1 Notes on this Setup Instruction ........................................................................................ 4
1.2 Intended use...................................................................................................................... 4
2Safety instructions...................................................................................................... 6
2.1 General notes .................................................................................................................... 6
2.2 Safety markings on Biometra TOne.................................................................................. 6
2.3 Technical condition ........................................................................................................... 6
2.4 Requirements for the operating personnel...................................................................... 7
2.5 Safety instructions: transport and installation ................................................................. 7
2.6 Safety instructions –during operation ............................................................................. 7
2.6.1 Summary of safety instructions ........................................................................................ 7
2.6.2 Safety instructions: protection against explosion and fire............................................... 8
2.6.3 Safety instructions: electrical equipment ......................................................................... 8
2.6.4 Handling of samples, auxiliary and operating materials ................................................. 9
2.7 Safety instructions –maintenance and repair ................................................................. 9
2.8 Behavior during emergencies ........................................................................................... 9
3Device installation and commissioning.................................................................... 10
3.1 Scope of delivery ............................................................................................................. 10
3.2 Unpacking and inspection............................................................................................... 10
3.3 Commissioning................................................................................................................ 10
3.4 Setting the operating voltage ......................................................................................... 11
4Functional elements................................................................................................. 12
4.1 Biometra TOne front view .............................................................................................. 12
4.2 Biometra TOne rear view................................................................................................ 12
5Operation.................................................................................................................. 13

Basic information Biometra TOne
4 Version 01.21
1Basic information
1.1 Notes on this Setup Instruction
As an excerpt from the detailed device instructions, these setup instructions contain the
first steps of installation and the safety instructions to be observed. For all further steps,
please apply the detailed operating instruction, which is supplied in electronic form on a
USB stick. Alternatively, you can also find the operating instruction on the Analytik Jena
homepage in the download area of the corresponding device.
Biometra TOne is intended for operation by qualified specialist personnel observing the
device operating manual for the Biometra TOne software.
The operating manual contains information about the design and operation of Biometra
TOne and provides personnel familiar with PCR technology with the necessary know-
how for the safe handling of the device and its components. The operating manual fur-
ther includes notes on the maintenance and servicing of the device and potential causes
and remedies of any faults.
This instruction manual applies to all Biometra TOne devices from software version ME
1.70 –RE 1.46.
Safety instructions are indicated by pictographs and signal words. The type and source
of the hazard are stated together with notes on preventing the hazard.
The operating manual uses the following symbols and signal words to indicate hazards
or instructions. Safety instructions are always placed before an action.
WARNING
Indicates a potentially hazardous situation which might cause fatal or very serious inju-
ries (deformities).
CAUTION
Indicates a potentially hazardous situation which might cause light or minor injuries.
NOTE
Provides information on potential material or environmental damage.
1.2 Intended use
The Biometra TOne is an end-point thermal cycler. It is designed to amplify nucleic acids
by repeated cycles of heating and cooling using DNA polymerases in a PCR reaction.
The thermal cycler is developed for Research Use Only (RUO).
The TOne combines modern design with user-friendly software. The user interface con-
sists of a touchscreen with a graphical or spreadsheet display that shows the time, the
status and the temperature program for each run. Using the touchscreen keyboard,
Conventions
Symbols and signal words

Biometra TOne Basic information
Version 01.21 5
information and program parameters can be entered directly on the screen. Due to the
high ramp rates, the TOne thermal cycler is suitable for fast PCR applications, thereby
helping to shorten program run times.
Deviations from the intended use described in this short instruction and in the operating
manual result in limitations of warranty and liability during a damage event. Damage to
wearing parts is not included in the warranty.
If the safety instructions given in this setup instruction and in the operating manual are
not observed when handling the device, this is considered a deviation from the intended
use.

Safety instructions Biometra TOne
6 Version 01.21
2Safety instructions
2.1 General notes
For your own safety and to ensure error-free and safe operation of Biometra TOne,
please read this chapter carefully before commissioning.
Besides the safety instructions in the operating manual and the local safety regulations
that apply to the operation of the device, the general applicable regulations regarding
accident prevention, occupational health and safety, and environmental protection have
to be observed and complied with.
References to potential hazards do not replace the work protection regulations which
must be observed.
2.2 Safety markings on Biometra TOne
Damaged or missing safety symbols can cause incorrect actions leading to personal in-
jury or material damage. Do not remove safety symbols! Replace damaged safety sym-
bols immediately!
The following safety symbols are attached to the inside of the heated lid and the rear of
the device:
Symbol
Meaning
Notes
Caution!
Hazard point
This symbol is located on the inside of the heated lid and on
the rear of the device.
Proceed with due care when handling samples and working
with Biometra TOne.
Caution!
Risk of burns
This symbol is located on the inside of the heated lid and on
the rear of the device.
There is a risk of burning at the heated lid, the thermal block,
the samples, and the rear of the device.
Ensure ventilation slots are
unobstructed
This sign is attached to the underside of the device.
Ensure that the ventilation slots on the underside and at the
rear of the device are unobstructed. Make sure that there are
no objects underneath the device (e.g., paper) that may be
sucked against the ventilation slot and thus interfere with
ventilation.
2.3 Technical condition
Biometra TOne has been built and certified according to safety standard EN 61010-1.
Do not modify the device in any way. Any modification made to the device will lead to a
loss of warranty and the EN 61010-1 certificate and poses a potential risk.

Biometra TOne Safety instructions
Version 01.21 7
The following has to be observed:
The operator must only operate the device in a sound and operationally safe condi-
tion. The technical condition must always comply with the legal requirements and
regulations.
Prior to every use the device must be checked for damage and sound condition.
Any changes in the device affecting its safety must be reported by the operating per-
sonnel to the operator without delay.
2.4 Requirements for the operating personnel
Biometra TOne must only be operated by qualified specialist personnel instructed in the
use of the device. The instruction also includes imparting the contents of the operating
manual.
In addition to the safety at work instructions in the operating manual, the generally ap-
plicable safety and accident prevention regulations of the respective country of opera-
tion must be observed and adhered to. The operator must ascertain the latest version of
these regulations.
The operating manual must be accessible to the operating and service personnel at all
times.
2.5 Safety instructions: transport and installation
The following has to be observed:
Drain the sample block before transporting Biometra TOne. Make sure there are no
sample tubes in the block.
Only send Biometra TOne in its original packaging.
On receipt of Biometra TOne, please verify that the delivery is complete and check
for possible transport damage. In the event of a claim, please contact Analytik Jena.
The Biometra TOne is designed for indoor use only and not for installation and oper-
ation in a wet environment.
2.6 Safety instructions –during operation
2.6.1 Summary of safety instructions
Before each commissioning, the operator of Biometra TOne must make sure that the
condition of the device including the safety equipment is sound.
The following has to be observed:
Free access to the main power switch on the rear panel of device must always be en-
sured during operation.
The ventilation fittings at the rear of the device must be unobstructed and opera-
tional. Covered ventilation grilles or slots etc. may cause the device to break down or
may cause damage to it.

Safety instructions Biometra TOne
8 Version 01.21
The use of oil between the samples and the sample block is not necessary to achieve
an improved heat exchange. However, if you still want to use oil, you should use
mineral oil. Do not use silicone oil.
Be careful to avoid crushing or pinching injuries when closing the device.
Biometra TOne may cause burns. The following has to be observed:
The thermal block, the samples, and the heated lid reach high temperatures. There is
a risk of burns during contact.
Ensure that the lid is securely closed before starting the program! Do not touch the
heated lid!
The rapid heating of the thermal block can cause liquids to boil explosively. Always
wear safety goggles during operation!
Do not touch hot sample tubes or plates and do not open them or boiling liquid may
escape!
Only use plates and tubes that are suitable for high temperatures (up to 100 °C), fit
well into the thermal block (no wobbling), and whose lids seal tightly!
Modifications and extensions to the device may only be carried out after consultation
with Analytik Jena. Unauthorized changes can limit the operating safety and lead to
restrictions in warranty and access to customer service.
2.6.2 Safety instructions: protection against explosion and fire
Biometra TOne must not be operated in an explosive environment. Biometra TOne must
not be operated with flammable, explosive, or volatile substances.
2.6.3 Safety instructions: electrical equipment
Work on the electrical components of Biometra TOne may only be performed by a quali-
fied electrical technician in accordance with the applicable electrical engineering rules.
Life-threatening electrical voltages may occur in the interior of Biometra TOne!
The following has to be observed:
Any work on the interior of the device may only be carried out by the Analytik Jena
service department and specially authorized technicians.
The electrical components must be checked regularly by a qualified electrician. Any
defects, such as loose connections, faulty, or damaged cables, must be repaired with-
out delay.
Before opening the device it must be switched off at the main power switch and the
power plug must be disconnected from the power outlet!
Biometra TOne must be switched off immediately using the main power switch and
the power plug disconnected from the power supply if any faults occur in the electric
components.
Do not operate the device near sources of strong electromagnetic radiation (e.g., a
non-shielded, deliberately operated high frequency source), as these may affect
proper operation of the device.

Biometra TOne Safety instructions
Version 01.21 9
2.6.4 Handling of samples, auxiliary and operating materials
The operator is responsible for the selection of substances used in the process as well as
for their safe handling. This is particularly important for radioactive, pathogenic, infec-
tious, poisonous, corrosive, or otherwise hazardous substances. For details contact the
safety officer responsible for your location. When handling hazardous substances local
safety codes and guidelines must be observed. The following general notes do not re-
place the specific local regulations or the regulations in the safety data sheets of the
manufacturers for the auxiliary and operating materials.
The following has to be observed:
Protective goggles and rubber gloves have to be worn when handing reagents.
If only a few samples are to be treated, two (empty) tubes of the same height must
additionally be placed in the corner positions of the block. If the number of samples
in the block is too low, there is a risk of the tubes being damaged and sample liquid
leaking out.
For your own safety, please consider the potential risk of infection of the examined
biological material.
2.7 Safety instructions –maintenance and repair
Biometra TOne is usually repaired by the Biometra GmbH/Analytik Jena AG service de-
partment or by authorized and trained specialist personnel. Unauthorized repairs can
damage the device. Therefore, the operator may generally only carry out the tasks listed
in the operating manual.
The following has to be observed:
The exterior of the device may only be cleaned with a damp, not dripping, cloth after
the device has been switched off.
Any maintenance on the device may usually only be carried out in the switched-off
condition (unless stated otherwise).
Use only original spare parts or consumables. Only these parts are tested and guar-
antee safe operation.
If the instrument is to be sent to the service department, please contact the Bi-
ometra/Analytik Jena service department beforehand. You will then receive an au-
thorization number. In addition, the device must be decontaminated before sending
it in and the corresponding form must be attached to the outside of the packaging,
see note in the detailed operating instructions.
2.8 Behavior during emergencies
During hazardous situations, Biometra TOne must be switched off immediately by oper-
ating the main power switch at the rear left of the controller. Disconnect the power plug
from the power supply!

Device installation and commissioning Biometra TOne
10 Version 01.21
3Device installation and commissioning
3.1 Scope of delivery
Thermal cycler:
Power cable
Quick Guide
Biometra TOne Setup Instructions
(print)
Biometra TOne operating manual (elec-
tronic, on Biometra TOne USB stick)
3.2 Unpacking and inspection
Unpack the device and check that it is complete and undamaged. Immediately report any
damage to Analytik Jena/Biometra.
WARNING
Do not commission the device if there are any signs of damage.
Keep the original packaging for return transport in the event that servicing is required.
The shipping box for the Biometra TOne thermal cycler contains specially developed
molded parts for the safe transport of high quality electronic devices.
3.3 Commissioning
Place the Biometra TOne thermal cycler on a solid, non-flammable surface in a se-
cure and dry environment. Consider the specific ambient conditions: 15 °C to 30 °C,
max. 70 % air humidity, max. 2000 m above sea.
Allow the Biometra TOne thermal cycler to equilibrate to room temperature before
switching it on.
Ensure adequate ventilation around the device, otherwise the device's performance
may be impaired. A minimum distance of 10 cm should be maintained behind the
thermal cycler between the rear of the device and a wall or other objects.
If several thermal cyclers stand next to each other and are operated simultaneously,
we recommend a lateral distance of at least 10 cm between the devices.
Make sure that the power switch on the controller and the power cable are easily ac-
cessible. This is important if the device needs to be disconnected from the power
supply.
Make sure that the ventilation slots at the sides and the bottom of the device are un-
obstructed.
Make sure that there are no objects underneath the device (e.g., paper) that may be
sucked against the ventilation slot and thus interfere with ventilation.

Biometra TOne Device installation and commissioning
Version 01.21 11
Insufficient ventilation may cause the de-
vice to overheat.
Before switching on the device, make sure that the power supply corresponds to the
device setting (see section "Setting the operating voltage" p. 11).
Connect the Biometra TOne thermal cycler controller to a grounded power outlet us-
ing the power cable supplied. The cable must not be replaced by an inadequately di-
mensioned power cable.
Check the operating voltage before switching on the device!
3.4 Setting the operating voltage
WARNING
Danger of electric shock! Before commissioning the device, make sure that the voltage
selector setting on the underside of the device corresponds to the power supply in your
laboratory.
The Biometra TOne thermal cycler can be operated at 100, 115 or 230 volts. The cur-
rent setting is indicated on the voltage selector switch on the underside of the controller.
To change the operating voltage, switch off the device and unplug the power plug.
Use a coin or other round object to rotate the voltage selector switch slot and adjust
the new operating voltage.

Functional elements Biometra TOne
12 Version 01.21
4Functional elements
4.1 Biometra TOne front view
Fig. 1 Biometra TOne front view
1 Dial for adjusting the contact pressure
2 Lid closing button
4 Display
6 USB port
4.2 Biometra TOne rear view
Fig. 2 Biometra TOne PCR module rear view
1 Ethernet port
2 Mains switch
3 Fuse holder
4 Mains plug

Biometra TOne Operation
Version 01.21 13
5Operation
For further information please check out the operating manual of the device. These
manuals are included in electronic form on USB flash drive. Alternatively, you can find
the operating manual on the Analytik Jena homepage in the download area of the corre-
sponding device.