Analytik Jena CyBio QuadStack Series User manual

Assembly Instructions
CyBio QuadStack
Microplate Storage

Manufacturer Analytik Jena GmbH
Konrad-Zuse-Straße 1
07745 Jena / Germany
Phone: [phone]
Phone: [phone]
Mail: [email protected]
Technical Service Analytik Jena GmbH
Konrad-Zuse-Straße 1
07745 Jena / Germany
Phone: [phone]
Fax: [phone]
E-mail: [email protected]
General Information http://www.analytik-jena.com
Documentation Number OL5003-26-00XBLE
Edition B (03/2023)
Technical Documentation Analytik Jena GmbH
© Copyright 2023, Analytik Jena GmbH
For a proper and safe use of this product follow the instructions. Keep the
operating manual for future reference.

CyBio QuadStack Table of contents
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Table of contents
1 Basic information ................................................................................................................................................. 5
1.1 About this manual ............................................................................................................................................ 5
1.2 Intended use ..................................................................................................................................................... 6
1.3 Standards and directives .................................................................................................................................. 6
2 Safety instructions................................................................................................................................................ 8
2.1 General.............................................................................................................................................................. 8
2.2 Safety markings ................................................................................................................................................ 8
2.3 Hazard zones and protective devices............................................................................................................... 9
2.3.1 Hazard zones .................................................................................................................................................... 9
2.3.2 Protective devices ............................................................................................................................................. 11
2.4 Requirements for the operating personnel..................................................................................................... 11
2.5 Device-specific safety instructions................................................................................................................... 11
2.5.1 Safety instructions for operation ..................................................................................................................... 11
2.5.2 Safety instructions: Transport.......................................................................................................................... 12
2.5.3 Safety instructions – maintenance and service............................................................................................... 12
2.6 Safety instructions ............................................................................................................................................ 12
2.6.1 Handling hazardous substances ...................................................................................................................... 12
2.6.2 Chemical resistance .......................................................................................................................................... 12
2.7 Behavior during emergencies .......................................................................................................................... 13
3 Technical description............................................................................................................................................ 14
3.1 Design................................................................................................................................................................ 14
3.2 Stacker shaft variants ....................................................................................................................................... 17
3.3 Access modules................................................................................................................................................. 18
3.4 Type plate.......................................................................................................................................................... 20
4 Installation and commissioning ........................................................................................................................... 21
4.1 Location requirements ..................................................................................................................................... 21
4.2 Initial commissioning and configuration......................................................................................................... 21
4.3 Re-commissioning............................................................................................................................................ 22
5 Operation.............................................................................................................................................................. 23
5.1 Switching on ..................................................................................................................................................... 23
5.2 Manual operation............................................................................................................................................. 23
5.3 Operation via control software ........................................................................................................................ 24
5.4 Loading labware to the shafts ......................................................................................................................... 24
5.5 Switching off ..................................................................................................................................................... 26
6 Troubleshooting ................................................................................................................................................... 27
6.1 General information on troubleshooting ........................................................................................................ 27
6.2 Behavior after fault reports.............................................................................................................................. 27
6.3 Fault removal.................................................................................................................................................... 28

Table of contents CyBio QuadStack
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7 Maintenance and care.......................................................................................................................................... 29
7.1 Safety instructions ............................................................................................................................................ 29
7.2 Maintenance overview ..................................................................................................................................... 30
7.3 Cleaning the device........................................................................................................................................... 30
8 Transport and storage.......................................................................................................................................... 31
8.1 Transport........................................................................................................................................................... 31
8.2 Storage .............................................................................................................................................................. 32
9 Disposal ................................................................................................................................................................ 33
10 Spare and wear parts, accessories, consumables ................................................................................................ 34
11 Technical data ...................................................................................................................................................... 35
Glossary ................................................................................................................................................................ 38

CyBio QuadStack Basic information
5
1 Basic information
1.1 About this manual
The manual describes the rotating microplate storage CyBioQuadStack with the models:
¡CyBioQuadStackM
¡CyBioQuadStackL
Depending on the configuration, the microplate storage includes an access module:
¡Lift Link Module
¡Lift Turn Lift Module
The original manual is written in German. Editions in other languages are translated
from the original manual.
The operating manual provides information about the design and operation of the de-
vice and provides operating personnel with the necessary know-how for safe handling of
the device and its components. Furthermore, the operating manual includes information
on the maintenance and servicing of the device as well as information on potential
causes of malfunctions and their correction.
Conventions Instructions for actions occurring in chronological order are numbered and combined
into action units.
Warnings are indicated by a warning triangle and a signal word. The type, source and
consequences of the hazard are stated together with notes on preventing the hazard.
Elements of the control and analysis program are indicated as follows:
¡Program terms are in bold (e.g., the System menu).
¡Menu items are separated by vertical lines (e.g., System|Device).
Symbols and signal words used
in this manual
The user manual uses the following symbols and signal words to indicate hazards or in-
structions. These warnings are always placed before an action.
WARNING
Indicates a potentially hazardous situation which can cause death or very serious (possi-
bly permanent) injury.
CAUTION
Indicates a potentially hazardous situation which can cause slight or minor injuries.
NOTICE
Provides information on potential material or environmental damage.

Basic information CyBio QuadStack
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1.2 Intended use
The operator is responsible to use the device as intended.
The microplate storage is designed for the automatic processing of labware in combina-
tion with other automatic devices in chemical and biological laboratories. In the field of
medicine and diagnostics its use is limited to research.
Its basic functions are picking up, temporarily holding and transferring labware to vari-
ous different automated transport systems or robots via access modules.
It is possible to manually load and unload the device through the stacker shaft doors. It
is, however, not permitted to transfer labware to the access module by hand.
The device can be configured with various access modules.
The labware that can be processed with the device are microplates in ANSI/SLAS format,
including deep-well and rigid full-skirted PCR plates. It is not possible to process flexible
full-skirted PCR plates and half-skirted PCR plates with this device.
Furthermore, the device is able to handle 96/250µL, 96/50µL and 192/60µL tip
boxes provided by the company Analytik Jena in filled or unfilled condition and without
lid. 96/1000µL tip boxes provided by the company Analytik Jena cannot be handled
with the device.
Please observe the following:
¡The device must only be operated by qualified and trained personnel.
¡The device must only be used in accordance with this manual. This applies in partic-
ular to the adherence to the connection values, conditions of use and notes on the
maintenance, transport, and disposal.
¡The safety instructions in this manual must be observed.
It is not permissible
¡to operate this equipment in a medical laboratory,
¡to work with explosive substances in this device,
¡to operate this device in an explosive environment.
¡to smoke or use a naked flame at the installation location.
As regards the safe handling of dangerous substances (radioactive, infectious, toxic, cor-
rosive, combustible, and other hazardous substances), the owner/operator will be re-
sponsible in accordance with applicable laws and guidelines.
The same applies in terms of compliance with environmental protection rules (e. g. for
disposal of reagents and consumables).
The device may only be used for the processes described in the assembly instructions.
Only the specified use is regarded to be the intended use. Using the device for any other
purpose may compromise the safety of the user and the device.
1.3 Standards and directives
The device was manufactured according to the currently applicable generally recognized
codes of practice and the generally accepted safety-related regulations. The relevant
safety and health requirements of the applicable laws, standards and regulations were
applied during the construction of the device.
EU directives The device is regulated by the directive 2006/42/EG and is considered an unfinished
machine. This means that the safety requirements are specified in the declaration of in-
corporation.

CyBio QuadStack Basic information
7
Furthermore, the following applies in this context: The unfinished machine may only be
put into operation if it has been determined that the machine or installation into which
the unfinished machine is to be incorporated complies with the provisions of the direc-
tive 2006/42/EG and that a corresponding declaration of conformity in accordance with
annexII, part1, sectionA has been issued.
The device meets the requirements of the directives 2014/30/EU and 2011/65/EU.
Any information regarding safety corresponds to the currently valid regulations of the
European Union. Other specific national laws and regulations must be observed.
Guard all hazard zones described in this manual for the safe integration of the device
into a machine or installation as far as possible from unintentional access. Preferably,
use a separating protective device to do this.
If the unfinished machine is incorporated into an external safety circuit via an E-stop ca-
ble, it is not permissible to have an E-stop jumper within the overall system.

Safety instructions CyBio QuadStack
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2 Safety instructions
2.1 General
For your own safety and to ensure error-free and safe operation of the device, please
read this chapter carefully before commissioning.
Screen Observe all safety instructions listed in these instructions, as well as all messages and in-
structions displayed by the control and analysis software on the monitor.
Besides the safety instructions in these instructions and the local safety regulations that
apply to the operation of the device, the general applicable regulations regarding acci-
dent prevention, occupational health and safety and environmental protection have to
be observed and complied with.
2.2 Safety markings
Warning and mandatory action labels have been attached to the device and must always
be observed.
Damaged or missing warning and mandatory action labels can cause incorrect actions
leading to personal injury or material damage. The labels must not be removed. Dam-
aged warning and mandatory action labels must be replaced immediately!
The following warning and mandatory action labels have been attached to the device:
Vor Öffnen des Gerätes
Netzstecker ziehen.
Before opening
disconnect mains.
Avant d'ouvrir l'appareil
retirez la fiche mâle.
Fig.1 Safety labeling on the device
Warning symbol Meaning Comment
Warning against danger-
ous electrical voltage
On the terminal block: Never open the
device. All repairs must be performed by
qualified personnel only.
Only replace defective fuses with fuses
with the specified rating.

CyBio QuadStack Safety instructions
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Warning symbol Meaning Comment
Warning of crushing On all stacker shafts, on the access
module: Warning of hand injuries
caused by moving device components
Warning: automatic start On all stacker shafts: Warning of auto-
matically rotating stacker shaft during
ongoing processes
Crushing injuries warn-
ing.
It is forbidden to reach
into the device.
Unplug the power cord
before carrying out any
maintenance work on the
device.
Risk of irreversible hand injuries due to
shearing and crushing caused by moving
device components in the area of the
lower stacker shaft opening and the ver-
tical lifter of the access module.
Unplug the power cord before carrying
out any maintenance work on the de-
vice.
The following characters can be found on the protective cover preventing the operator
from reaching into the access module:
Character Meaning Comment
Warning of crushing Warning of hand injuries caused by
moving device components
It is forbidden to reach
into the device
Do not reach into the range of move-
ment of the access module while the
device is running
Unplug the power cord
before servicing the de-
vice
Before performing any maintenance
work in the area around the device,
switch off the device and disconnect the
mains plug from the mains socket.
2.3 Hazard zones and protective devices
2.3.1 Hazard zones
The rotary movement of the stacker shaft and the movement of the access module may
put the operating personnel at risk. Operating the device without its own protective
housing or without the protective housing of the overall system in place does not comply
with its intended use and is therefore prohibited.
Not complying with the instructions on the warning notices can lead to serious injury to
the hands caused by crushing or shearing. Any interference with the device during oper-
ation can result in damage to the device and to the samples.
Devices with their own protective housing have a transparent protective housing around
the stacker shaft which ensures that the process is safe for the operating personnel. The
protective housing can be opened on the main operating side via a monitored door.
The access module is located behind a transparent protective shield preventing the op-
erator from reaching into the device. There is a risk of crushing and shearing fingers and
hands at the lower stacking shaft opening.

Safety instructions CyBio QuadStack
10
1
2
3
Fig.2 Hazard zones
1 Range of rotation of the stacker shaft 2 Passage area of the transport system
3 Range of movement of the access
module
Warning labels ¡Never use the device without its protective housing.
¡In the event of malfunction, first switch off the device. Unplug the power cord be-
fore, for example, removing microplates that got stuck or clamped inside the device.
¡Never reach into the range of movement of the stacker shaft, the access module or
any transport system with your hands or any type of object while the device is run-
ning. There is a particular risk of injury at the gaps at the 4 stacker shaft doors and
at the lower stacker shaft opening. There is a risk of irreversible hand injuries caused
by crushing or shearing.
¡To abort a program, press the STOP button. All drives stop.
¡The stacker shaft only rotates when the doors are closed. The position of the doors
is monitored by a magnet.
¡Always correct any incorrect movement with the aid of the PC. Incorrect handling
and operation can result in material damage and personal injury.

CyBio QuadStack Safety instructions
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2.3.2 Protective devices
1
Fig.3 STOP button
Press the STOP button (1 - see figure) to bring all drives to an immediate stop.
2.4 Requirements for the operating personnel
The device may only be operated by trained specialist personnel instructed in technical
safety. The operating personnel must have read and understood the operating instruc-
tions.
The personal protective equipment must be worn to operate or service the device.
The operating personnel must be familiar with the dangers arising from the substances
used.
2.5 Device-specific safety instructions
The system must be installed by the service personnel of the manufacturer or duly
trained and authorized expert personnel under any circumstances.
Do not use aggressive substances of a type that may compromise the stable perfor-
mance of the system
Before connecting to the mains, check the electrical requirements of the device.
Observe prescribed maintenance intervals!
Only use the accessory items, consumables and spare parts specified in this document or
provided or recommended by the manufacturer!
2.5.1 Safety instructions for operation
The operator of the device must ensure that the device is in sound condition before each
use. This applies especially after any modification or adaptation of the device or any re-
pair.
Do not operate the system with defective safety devices or with improperly installed
safety and protection devices.
Do not remove, modify or disable any safety and protection devices during operation.
Ensure easy access to the main power switch, as well as to any emergency shutdown
systems and locks at all times during operation.
Ensure that all ventilation devices on the device are in proper functional condition. Cov-
ered ventilation grates or slots, etc. can result in malfunctions or device damage.

Safety instructions CyBio QuadStack
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Only operate the device when connected to a power socket with grounding conductor.
The grounding conductor must not be interrupted (e.g., when using a voltage regulating
transformer). Only use extension cables equipped with grounding conductors!
When replacing the power cable, ensure that the new power cable has the proper di-
mensions for the intended operating voltage (see technical data).
Do not insert any objects into any device openings, and ensure that no liquid can get
into the device through openings or joints.
Do not short-circuit the device fuses and only use fuses corresponding to the informa-
tion in these instructions when replacing these.
2.5.2 Safety instructions: Transport
Only transport the device and its components in the original packaging! Ensure that all
transport locks and safety devices have been fitted and that the device components are
fully emptied and decontaminated if applicable.
2.5.3 Safety instructions – maintenance and service
Service and repairs and work for the commissioning or dismantling the device for trans-
port must only be carried out by authorized service personnel!
The operator may only carry out the tasks listed in the chapter "Maintenance and care".
Only carry out maintenance and service work on the device when it is switched off. Dis-
connect the power cord from the mains socket beforehand.
2.6 Safety instructions
2.6.1 Handling hazardous substances
Even with intended use there is a risk of health damage when handling hazardous sub-
stances. The operator is solely responsible for the compliance with all safety require-
ments to protect individuals and property when handling radioactive, infectious, toxic,
caustic, flammable and other hazardous substances.
¡Control the handling of hazardous substances in accordance with the safety category
of the lab, the details in the safety data sheets of the respective substances, the
manufacturer recommendations for use and additional national and international
regulations (WHO, “Laboratory Biosafety Manual").
¡Wear personal protective equipment when working with the device.
¡Observe all notices on the cleaning and decontamination of the device.
2.6.2 Chemical resistance
Aggressive substances may damage the device. Although the materials used are resis-
tant to most of the commonly used substances, material damage from aggressive sub-
stances cannot be completely excluded.
¡Before using any aggressive substances (e.g., bases, acids or organic solutions):
Check that the materials with direct contact to these substances are resistant.
¡When in doubt, consult the manufacturer.

CyBio QuadStack Safety instructions
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Prohibited substances
Hydrofluoric acid (HF/hydrofluoric acid)
Highly concentrated acids
Cleaning powder
Paint thinner
Naphtha (crude gasoline)
Gasoline
Acetone
Cleaning spray
Ozone
¡Substances not listed in this table are not necessarily suitable.
¡Do not use solvents (thinners), aggressive detergents, flammable liquids or caustic
alkaline solutions for cleaning. These can lead to damage to the housing compo-
nents.
Disinfection method Disinfectant Can be used for
Wipe disinfection Incidin Liquid (ECOLAB) – Housing parts
– Accessories
Table1 Permissible disinfection methods and disinfectants
2.7 Behavior during emergencies
In hazardous situations or in the event of an accident, immediately use the main power
switch or actuate the emergency stop pushbutton to turn the device off, then pull the
mains plug from the mains socket.

Technical description CyBio QuadStack
14
3 Technical description
3.1 Design
The compact and flexible microplate storage has a 4-fold stacker shaft. The stacker shaft
is used to hold, pick up and dispense various types of labware.
7
8
5
3
1
4
6
2
Fig.4 Device configuration
1 Rotating 4-fold stacker shaft 2 Stack door
3 Bottom plate 4 Protective housing with door
5 Control unit 6 Access module
7 Type plate 8 Connector panel
A sensor monitors the stacking process during stacking and unstacking. A second sensor
detects empty stacker shafts.
The rotational movement of the stacker shafts can also be initiated manually. A control
panel with buttons and a status LED is located on the main operating side.
Electromechanical components and sensors are integrated in the lower section of the
housing. The stacker shaft with its 4 shafts mounted on its rotatable bearing is located
on the housing. The rotating unit has a protective housing. The door of this protective
housing and the doors of the individual shafts are monitored by sensors.
The labware that can be processed with the device are microplates in ANSI/SLAS format,
including deep-well and rigid full-skirted PCR plates. It is not possible to process flexible
full-skirted PCR plates and half-skirted PCR plates with this device.

CyBio QuadStack Technical description
15
Furthermore, the device is able to handle 96/250µL, 96/50µL and 192/60µL tip
boxes provided by the company Analytik Jena in filled or unfilled condition and without
lid. 96/1000µL tip boxes provided by the company Analytik Jena cannot be handled
with the device.
Labware is inserted into or taken out of one of the shafts using various access modules.
Depending on the design, the access module will transfer the labware to another trans-
port device such as a robot or a transport system.
To ensure error-free and safe operation, the microplate must lie flat on the contact sur-
face of the access module. The edge of the plate must be positioned within the pins of
the access module.
In normal operation, the device is operated via a PC. You can stack the labware manually
into the shaft to prepare a process. Alternatively, additional devices such as robots can
automatically insert the labware into the shafts via access modules.
There are different protective housings for operating the microplate storage with vari-
ous main devices which can be opened to the left or to the right, for example. Device
variants without their own protective housing are available from Analytik Jena for inte-
grating the device into a complete system. In this case, the devices must be integrated
into the protective housing of the system.

Technical description CyBio QuadStack
16
Connections
1
2
2
3
4
5
6
7
8
9
10
11
12
13
14
Fig.5 Connections
1 ESTOP 1 -
Connection of the STOP button to the
previous CyBio QuadStack
2 SENS2 -
External sensor
3 SENS1 -
External sensor
4 COM2 -
Main device
5 COM1 -
Previous device
(e.g. CyBio QuadStack)
6 LED -
Status (service only)
7 SW -
Switch output
8 INPUT - Power socket
9 Service -
Service interface
10 ESTOP2 -
Connection of the STOP button to the
subsequent CyBio QuadStack
11 SENS3 -
External sensor
12 COM3 -
Subsequent device
(e.g. CyBio QuadStack)
13 BC -
Barcode reader
14 Mains switch

CyBio QuadStack Technical description
17
Control elements
123
4
Fig.6 Control unit
1 Button -
Clockwise rotation
2 STOP button
3 Button -
Counter-clockwise rotation
4 LED –
Operating status display
Operating status display
The device front houses the control panel with an operating status display. This indi-
cates the current operating status of the device:
Display Operating status
GREEN The device is ready for operation. It can be
operated manually or via PC.
ORANGE The device is working.
A process is currently being carried out.
RED The device indicates that a shaft door is not
closed or an error is present.
3.2 Stacker shaft variants
In normal operation, the microplate storage is controlled and operated via a PC. It is
possible to insert individual microplates, plate stacks or tip boxes equipped with pipette
tips into the rotating stacker shaft. Verify that the labware actually fits into the stacker
shafts.
The doors on the stacker shafts are monitored via an electromagnet. Stacker shafts can
only rotate when the doors are closed.
It is possible to manually or automatically load the 4 stacker shafts with labware before
the device is put into operation. Various different access modules are available for that
purpose.
The microplate storage may be equipped with stacker shafts of different lengths:
¡CyBioQuadStackM (shaft length 555mm)
¡CyBioQuadStackL (shaft length 755mm)
Microplate capacity Device model Maximum number of microplates/shaft
by height of microplates
Microplate height 9mm 14.6mm 44mm
CyBioQuadStackM 69 43 13
CyBioQuadStackL 94 58 18

Technical description CyBio QuadStack
18
3.3 Access modules
The access module serves the following purposes:
¡Placing labware into the stacker shafts
¡Removing labware from the stacker shafts
¡Transferring labware to another transport device such as a robot
¡Rotating the labware (Lift Turn Lift Module only)
Electromagnetically controlled latches retain the microplates inside the stacker shafts. If
one shaft is filled, the device will rotate to the next shaft.
Depending on the configuration, the device is equipped with the following access mod-
ules:
¡Lift Link Module
¡Lift Turn Lift Module
1
2
Fig.7 Device with access modules
1 Lift-turn-lift module 2 Lift-link module
Lift Link Module Access modules are used when you combine a microplate storage with a main unit, such
as an automatic pipetting device (e.g. CyBioWellvario) and a transport system in linear
or circular arrangement.

CyBio QuadStack Technical description
19
1
23
Fig.8 Lift Link Module
1 Lifter 2 Housing
3 Interface (on the rear)
The access module lifts the labware out of the transport system and loads the stacker
shafts via the lower stacker shaft opening. The lifter places the labware inside the shaft
onto the electromagnetic latches.
For removing labware from the microplate storage, the lifter will first lift up the labware
slightly. The latches are retracted. The lifter places the labware onto the transport sys-
tem. The transport system will then move the labware on rails below the stacker shaft,
for example, to move it to the main unit.
Lift Turn Lift Module The access module is very flexible. That is why it is often used in more complex installa-
tions.
3
2
1
4
5
Fig.9 Lift Turn Lift Module
1 Centering pin 2 Lifter
3 Rotating unit 4 Housing
5 Interface (on the rear)
The access module can perform three different types of movement:
¡Lift: The lifter lifts the labware to place it in the stacker shaft or to dispense it from
there.
¡Turn: The tray rotates the labware.
¡Lift: The tray lifts the labware to a height configured in the control software in order
to automatically transfer the labware to a robot, for example.

Technical description CyBio QuadStack
20
The labware can be taken out of the access module by the gripper of a laboratory robot
such as CyBioCarry, for example.
Fig.10 CyBioQuadStack in an installation with Analytik Jena devices
3.4 Type plate
Type plates are located on the terminal strip of the device as well as on the back of the
access module.
The type plate contains the following information:
¡Manufacturer address, trademark
¡Machine designation
¡Serial number
¡Conformity markings
¡Year of manufacture
¡WEEE marking