Beckman Coulter Optima L Series User manual

PN LL-IM-12AB
October 2013
Instructions For Use
Optima L Series
Preparative Ultracentrifuges
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.

Optima L Series
Preparative Ultracentrifuges
PN LL-IM-12AB (October 2013)
Copyright © 2013 Beckman Coulter, Inc.
All rights reserved
No part of this document may be reproduced or
transmitted in any form or by any means,
electronic, mechanical, photocopying, recording, or
otherwise, without prior written permission from
Beckman Coulter, Inc.
Beckman Coulter and the stylized logo are trademarks of
Beckman Coulter, Inc. and are registered in the USPTO.
All other trademarks, service marks, products, or services
are trademarks or registered trademarks of their
respective holders.
Find us on the World Wide Web at:
www.beckmancoulter.com
Made in U.S.A.
Printed in U.S.A.

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Safety Notice
Read all product manuals and consult with Beckman Coulter-trained personnel before attempting
to operate instrument. Do not attempt to perform any procedure before carefully reading all
instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as
to how to proceed in any situation, contact your Beckman Coulter Representative.
Alerts for Warning, Caution, and Note
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided,
could result in death or serious injury.
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided,
may result in minor or moderate injury and/or mechanical damage. It may also be
used to alert against unsafe practices.
NOTE
NOTE is used to call attention to notable information that should be followed during installation, use,
or servicing of this equipment.
Safety During Installation and/or Maintenance
This instrument is designed to be installed by a Beckman Coulter Field Service representative.
Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty
covering the instrument. Also, should the instrument need to be moved, a Beckman Coulter Field
Service representative must reinstall and relevel the instrument in its new location.
Any servicing of this equipment that requires removal of any covers can expose parts which involve
the risk of electric shock or personal injury. Make sure that the power switch is turned off and the
instrument is disconnected from the main power source by removing the Mains (power) plug from
the outlet receptacle, and refer such servicing to qualified personnel.
Do not replace any centrifuge components with parts not specified for use on this instrument.

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Safety Notice
Electrical Safety
Electrical Safety
To reduce the risk of electrical shock, this instrument uses a three-wire electrical cord and plug to
connect this equipment to earth-ground. Make sure that the matching wall outlet receptacle is
properly wired and earth-grounded.
Do not place containers holding liquid on or near the chamber door. If they spill, liquid may get into
the instrument and damage electrical or mechanical components.
Safety Against Risk of Fire
Fuses protect certain electrical circuits within this instrument against overcurrent conditions. For
continued protection against the risk of fire, replace only with the same type and rating specified.
This instrument is not designed for use with materials capable of developing flammable or
explosive vapors or hazardous chemical reactions. Do not centrifuge such materials (for example,
chloroform or ethyl alcohol) in this instrument nor handle or store them near the centrifuge.
Mechanical Safety
For safe operation of the equipment, observe the following:
• Use only the Beckman Coulter rotors and accessories designed for use in this instrument.
• Do not exceed the maximum rated speed of the rotor in use.
• NEVER attempt to slow or stop a rotor by hand.
• Do not move the centrifuge while the drive motor is spinning.
• In the event of a power failure, do not attempt to retrieve the sample from the instrument for
at least one hour. Then follow the instructions for recovery of the sample under CHAPTER 4,
Troubleshooting and Maintenance.
Chemical and Biological Safety
Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or
radioactive. Such materials should not be used in this instrument, however, unless all necessary
safety precautions are taken.
• Handle body fluids with care because they can transmit disease. No known test offers complete
assurance that they are free of micro-organisms. Some of the most virulent—Hepatitis (B and
C) and HIV (I-V) viruses, atypical mycobacterium, and certain systemic fungi—further
emphasize the need for aerosol protection. Handle other infectious samples according to good
laboratory procedures and methods to prevent spread of disease. Because spills may generate
aerosols, observe proper safety precautions for aerosol containment. Do not run toxic,
pathogenic, or radioactive materials in a rotor without taking appropriate safety precautions.

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Safety Notice
Chemical and Biological Safety
Biosafe containment should be used when Risk Group II materials (as identified in the World
Health Organization Laboratory Biosafety Manual) are handled; materials of a higher group
require more than one level of protection.
• Dispose of all waste solutions according to appropriate environmental health and safety
guidelines.
It is your responsibility to decontaminate the instrument and accessories before requesting service
by Beckman Coulter Field Service.

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vi
Safety Notice
Chemical and Biological Safety

vii
Contents
Safety Notice,iii
Alerts for Warning, Caution, and Note, iii
Safety During Installation and/or Maintenance,iii
Electrical Safety,iv
Safety Against Risk of Fire,iv
Mechanical Safety,iv
Chemical and Biological Safety,iv
Introduction,xiii
Instrument Certification, xiii
Scope of Manual, xiii
Conventions,xiv
Recycling Label,xiv
RoHS Notice,xv
Specifications,xvi
Preinstallation Requirements, xviii
CHAPTER 1: Description, 1-1
Introduction,1-1
Power,1-1
Standby Mode,1-1
Key Switch Positions,1-1
Control Panel,1-2
Keys,1-2
Displays,1-2
Run Status Indicators,1-3
Diagnostic Messages,1-4
Door,1-4
Rotor Chamber,1-4

viii
Contents
Vacuum System,1-4
Temperature Sensing,1-5
Temperature Control System,1-5
Overspeed,1-6
Rotor Energy System,1-6
Drive,1-6
Name Rating Plate,1-6
CHAPTER 2: Run Preparation,2-1
Introduction,2-1
Power,2-1
Key Switch Positions,2-1
Standard Operation,2-2
Keypad,2-2
Parameter Keys,2-3
Programmed Operation,2-7
Activation Keys,2-8
Delayed Start Program Function,2-10
Entering Program,2-10
Running Program 0 with a Second Program,2-11
Editing Settings During the Delayed Start Run,2-11
CHAPTER 3: Run Procedure,3-1
Introduction,3-1
Standard Operation,3-1
Repeating a Run,3-2
Programmed Operation,3-2
Delayed Start Program Function,3-3
Zonal Operation,3-4
Acceleration,3-5
Deceleration,3-5
Unloading,3-5
Points to Remember,3-6
CHAPTER 4: Troubleshooting
and Maintenance,4-1
Introduction,4-1
In Case of Power Failure During Run,4-1

ix
Contents
Retrieving Your Sample,4-1
Diagnostic Messages,4-5
Cleaning,4-7
Decontamination,4-7
Sterilization and Disinfection,4-7
Storage and Transportation,4-8
Supply List,4-8
Beckman Coulter, Inc.
Optima L, LE, L-XP, and XL Preparative Ultracentrifuges
Warranty
Related Documents

x
Contents

xi
Illustrations
Illustrations
1.1 The Control Panel,1-2
1.2 The Rotor Chamber,1-5
2.1 Inserting the Key Into the Interlock Switch,2-2
4.1 Removing the Control Head Cover,4-2
4.2 Depressing the Latch to Loosen the Front Panel,4-3
4.3 Interior View of Instrument (Door Removed),4-4
4.4 Location of Diagnostic Messages on the Control Panel,4-5

xii
Tables
Tables
1.1 Run Status indicators,1-3
2.1 Vacuum LEDs,2-9
4.1 Diagnostic Troubleshooting Chart,4-6

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Introduction
Instrument Certification
Beckman Coulter Optima L series ultracentrifuges are manufactured in a facility that maintains
certifications to both ISO 9001:2008 and ISO 1348:2003. They have been designed and tested to be
compliant (when used with Beckman Coulter rotors) with the laboratory equipment requirements
of applicable regulatory agencies. Declarations of conformity and certificates of compliance are
available at www.beckmancoulter.com.
Scope of Manual
This manual is designed to familiarize you with the Beckman Coulter Optima L-100K or L-90K
Preparative Ultracentrifuge—its function, specifications, operation, and routine operator care and
maintenance. Beckman Coulter recommends that you read this entire manual, especially the Safety
Notice and all safety-related information, before operatingthe instrument or performing
instrument maintenance.
• The following introductory pages contain the instrument specifications and preinstallation
requirements.
• CHAPTER 1, Description provides a brief description of the instrument, the operating controls,
and indicators.
• CHAPTER 2, Run Preparation explains how to use the control panel to enter run parameters for
standard and programmed operation.
• CHAPTER 3, Run Procedure covers operating procedures for both kinds of runs.
• CHAPTER 4, Troubleshooting and Maintenance lists possible malfunctions, together with
probable causes and corrective actions, and maintenance procedures.
NOTE
If the ultracentrifuge is used in a manner other than specified in this manual, the safety and
performance of this instrument could be impaired. Further, the use of any equipment other than that
intended for use by Beckman Coulter has not been evaluated for safety. Use of any equipment
not specifically recommended in this manual is the sole responsibility of the user.

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Introduction
Conventions
Conventions
Certain symbols are used throughout this manual to call attention to safety-related and other
important information. The symbols used are reproduced on the inside back cover of this manual.
Typographic Conventions
Certain typographic conventions are used throughout this manual to distinguish names of user
interface components, such as keys and displays.
• Key names (for example,
START or ENTER/RECALL ) and display names (for example, TEMP°C or
SPEED-RPM ) appear in bold type.
Recycling Label
This symbol is required in accordance with the Waste Electrical and Electronic Equipment (WEEE)
Directive of the European Union. The presence of this marking on the product indicates.
1. the device was put on the European market after August 13, 2005 and
2. the device is not to be disposed via the municipal waste collection system of any member state
of the European Union.
It is very important that customers understand and follow all laws regarding the proper
decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing
this label please contact your dealer or local Beckman Coulter office for details on the take back
program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal
of the device.
A28219-AA

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Introduction
RoHS Notice
RoHS Notice
These labels and materials declaration table (the Table of Hazardous Substance’s Name and
Concentration) are to meet People’s Republic of China Electronic Industry Standard SJ/T11364-2006
“Marking for Control of Pollution Caused by Electronic Information Products” requirements.
China RoHS Caution Label — This label indicates that the electronic information product contains
certain toxic or hazardous substances. The center number is the Environmentally Friendly Use
Period (EFUP) date, and indicates the number of calendar years the product can be in operation.
Upon the expiration of the EFUP, the product must be immediately recycled. The circling arrows
indicate the product is recyclable. The date code on the label or product indicates the date of
manufacture.
China RoHS Environmental Label — This label indicates that the electronic information product does
not contain any toxic or hazardous substances. The center “e” indicates the product is
environmentally safe and does not have an Environmentally Friendly Use Period (EFUP) date.
Therefore, it can safely be used indefinitely. The circling arrows indicate the product is recyclable.
The date code on the label or product indicates the date of manufacture.
STOP
VACUUM—
µ
SPEED—RPMTIME—HR:MINTEMP—°C
POWER OFF
X 10,000
SAVE
PROG
HOLD
w
2
t
DECELACCEL
VACUUM
START
STOP
TIME
TEMP
CE
0
123
456
789
SPEED
ENTER/RECALL

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Introduction
Specifications
Specifications
Control Panel
Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data,
without guarantee.
Specifications Description
Speed • Set speed:
—1000 rpm to maximum speed
a
in increments of 100 rpm
• Speed control:
—Actual rotor speed will be ±20 rpm of the set speed (above 1000 rpm)
• Speed display:
—Indicates rotor speed in increments of 10 rpm at speeds below 1000 rpm, and
increments of 100 rpm at speed above 1000 rpm
Time • Set time:
—Up to 99 hours and 59 minutes; HOLD for runs of unspecified length
• Time display:
—Indicates time remaining in timed runs, time elapsed in HOLD runs, and
estimated time remaining in ω
2
truns
Te m p e r a t u r e • Set temperature:
—0 to 40°C in increments of 1°C
• Te m p e r a t u r e c o n t r o l :
—±0.5°C of set temperature
• Te m p e r a t u r e d i s p l a y:
—Indicates rotor temperature in increments of 0.1°C
• Temperature stability
—Within 0.3°C of set after equilibration
• Ambient temperature range:
—15 to 40°C
ω
2
tIntegrator • Set ω
2
t
—Up to 9.99 ×10
14
radians squared per second
• ω
2
tdisplay
—Shows accumulated centrifugal force to three significant digits (in
exponential notation)
Acceleration Selection from two profiles: maximum acceleration from 0 rpm to set speed, or slow
acceleration from 0 to 500 rpm, followed by maximum acceleration to set speed
Deceleration Selection from three profiles: full dynamic braking to 0 rpm, slow (full brake to
500 rpm followed by reduced braking to a gentle stop), or no brake

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Introduction
Specifications
Operational Features
NOTE The Optima L series ultracentrifuges have been designed and tested to operate safely indoors at
altitudes up to 2000 m (6562 ft).
Physical Data
Operation Standard, programmed, or delayed start; instrument memory can store up to nine
programs
Key Switch Used to select normal or zonal operation
a. Maximum speed will be 100,000 rpm or 90,000 rpm for the current models. Models purchased earlier may include maximum
speeds of 80,000, 70,000, or 60,000 rpm.
Specifications Description
Specifications Description
Revolution
Counter
Displays accumulated total number of rotor revolutions in tens of thousands
Barrier Ring 41-mm (1.63-in.) heat-treated steel alloy armor ring surrounded by a 12-mm (0.50-in.)
steel vacuum chamber to provide full protection for the operator
Door 17.5-mm (0.69-in.) high-strength structural steel
Vacuum Diffusion pump in series with a mechanical pump typically reduces chamber pressure
to below 5 microns (0.7 Pa)
Instrument
Classification
S (uses all Beckman Coulter preparative rotors except Types 35 and 42.1 rotors with
serial numbers 1299 or lower)
Diagnostic
Messages
See CHAPTER 4, Troubleshooting and Maintenance
Specifications Description
Weight 465 kg (1025 lb)
Height (overall) 120.7 cm (47.5 in.)
Width 94.0 cm (37 in.)
Depth 67.3 cm (26.5 in.)
Ventilation clearances 5.1 cm (2 in.) sides; 15.2 cm (6 in.) rear
Finishes • Keypad
—coated polycarbonate
• To p s u r f a c e
—urethane paint
• Other surfaces
—acrylic baking enamel
Maximum heat dissipation into the room 1.0 kW (3400 Btu/hr)

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Introduction
Preinstallation Requirements
Preinstallation Requirements
CAUTION
Do not attempt to install or turn on the power to the instrument. Its purchase price
includes installation by Beckman Coulter personnel. Installation by anyone other
than authorized Beckman Coulter personnel invalidates the warranty.
Preinstallation requirements have been sent prior to shipment of the instrument. (Copies may also
be attached to the outside of the shipping container and are available at www.beckmancoulter.com.)
The following information is provided in case the ultracentrifuge must be relocated. Contact your
Beckman Coulter Field Service representative to adjust and level the instrument if it must be moved.
Electrical Data
To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord (1.8 m; 6 ft)
and plug to connect the equipment to earthground. In regions where the instrument is supplied
with an unterminated cord, a plug that meets local electrical and safety requirements must be
supplied. (Contact your local Beckman Coulter office for specific information regarding these
Humidity restrictions <95% (non-condensing)
Noise level measured 0.91 m (3 ft) in front of the
instrument
<57 dBa
Installation (overvoltage) category II
Pollution degree 2
a
a. Normally only nonconductive pollution occurs; occasionally, however, a temporary conductivity caused by condensation
must be expected
Specifications Description
Instrument rating: 220 to 240 VAC, 50 Hz, 20 A
200 to 240 VAC, 50/60 Hz, 20 A
Power line range: 180 to 264 VAC, 60 or 50 Hz (single-phase), 30 A
Electrical supply: Class I

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Introduction
Preinstallation Requirements
requirements.) See the Table below for the required wire connections. Make sure that the matching
wall outlet receptacle is properly wired and earth-grounded.
To ensure safety, the instrument should be wired to a remote emergency switch (preferably outside
the room where the centrifuge is housed, or adjacent to the exit from that room). In case of a
malfunction, the centrifuge can be disconnected from the main power source by removing the
Mains (power) plug from the outlet receptacle.
Optima L series ultracentrifuges have been designed and tested to operate safely indoors at
altitudes up to 2000 m (6562 ft).
Space
Locate the Optima L in a clean, safe, uncluttered environment. Be sure to provide a 5.1-cm (2-in.)
clearance on each side, as the feet extend about 5.1 cm (2 in.) beyond the instrument (see the Figure
below). (The pads under each foot have been designed to prevent possible rotation of the
instrument in the event of a rotor mishap.) A 15.2-cm (6-in.) clearance is required at the rear of the
instrument for servicing the control panel and to ensure sufficient air ventilation during operation.
The ultracentrifuge must have adequate air ventilation to ensure compliance to local requirements
for vapors produced during operation.
1. 30-ampere Circuit Protector
2. Measured Line Voltage
3. North American Plug
4. Wall Outlet Hubbell 9330, Bryant 96-30 FR,
or Equivalent (NEMA 6-30R)
5. Earth Ground
Required Wired Connection
Wire Insulation
Color Term in a l
Symbol
Harmonized North American
Green/Yellow Earth ground
Light Blue Neutral N L
Brown Live or Line L L
1
1
5
2
3
4

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Introduction
Preinstallation Requirements
The Mains (power) plug is the disconnect device and must remain easily accessible. Position the
ultracentrifuge so that it is easy to remove the Mains (power) plug from the outlet receptacle.
The instrument will operate within specifications in a laboratory with ambient temperatures
ranging from 15 to 40°C.
WARNING
Do not place the instrument near areas containing flammable reagents or
combustible fluids.
Bio-Safety Level 3 Installation
For laboratories with epoxy aggregate floors, such as BSL-3 labs, a non-invasive installation kit
(PN 393862) is available. The kit, which consists of a high-strength adhesive tape, is CSA certified for
use on epoxy aggregate floors only.
cm
in.
94.0
37
5.1
2
5.1
2
67.3
26.5
15.2
6