Beckman Coulter JA-25.50 User manual

PN J-TB-056FD
September 2016
Instructions For Use
JA-25.50
Fixed-Angle Rotor
For Use in Beckman Coulter
J2 and Avanti J Series Centrifuges
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.

JA-25.50 Fixed-Angle Rotor
PN J-TB-056FC (September 2016)
Copyright ©1995–2016 Beckman Coulter, Inc.
All rights reserved. No part of this document may be
reproduced or transmitted in any form or by any means,
electronic, mechanical, photocopying, recording, or
otherwise, without prior written permission from
Beckman Coulter, Inc.
Avanti, Beckman Coulter, and the stylized logo are
trademarks of Beckman Coulter, Inc. and are registered in
the USPTO.
All other trademarks, service marks, products, or services
are trademarks or registered trademarks of their
respective holders.
Find us on the World Wide Web at:
www.beckmancoulter.com
Printed in the U.S.A.

PN J-TB-056FD iii
Safety Notice
Read all product manuals and consult with Beckman Coulter-trained personnel before attempting
to use this equipment. Do not attempt to perform any procedure before carefully reading all
instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as
to how to proceed in any situation, contact your Beckman Coulter Representative.
Alerts for Warning, Caution, Important, and Note
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided,
could result in death or serious injury.
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided,
may result in minor or moderate injury. It may also be used to alert against unsafe
practices.
IMPORTANT
IMPORTANT is used for comments that add value to the step or procedure being performed.
Following the advice in the Important adds benefit to the performance of a piece of equipment or to a
process.
NOTE NOTE is used to call attention to notable information that should be followed during installation, use,
or servicing of this equipment.
Safety Information for the JA-25.50 Rotor
This rotor was developed, manufactured, and tested for safety and reliability as part of a
Beckman Coulter centrifuge/rotor system. Its safety or reliability cannot be assured if used in a
centrifuge not of Beckman Coulter’s manufacture or in a Beckman Coulter centrifuge that has been
modified without Beckman Coulter’s approval.
Handle body fluids with care because they can transmit disease. No known test offers complete
assurance that such fluids are free of micro-organisms. Some of the most virulent—Hepatitis (B and
C) viruses, HIV (I–V), atypical mycobacteria, and certain systemic fungi—further emphasize the
need for aerosol protection. Handle other infectious samples according to good laboratory
procedures and methods to prevent spread of disease. Because spills may generate aerosols,
observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or
radioactive materials in this rotor without taking appropriate safety precautions. Biosafe
containment should be used when Risk Group II materials (as identified in the World Health
Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more
than one level of protection.

PN J-TB-056FD
iv
Safety Notice
Safety Information for the JA-25.50 Rotor
The rotor and accessories are not designed for use with materials capable of developing flammable
or explosive vapors. Do not centrifuge such materials in nor handle or store them near the
centrifuge.
Although rotor components and accessories made by other manufacturers may fit in the JA-25.50
rotor, their safety in this rotor cannot be ascertained by Beckman Coulter. Use of other
manufacturers’ components or accessories in the JA-25.50 rotor may void the rotor warranty and
should be prohibited by your laboratory safety officer. Only the components and accessories listed
in this publication should be used in this rotor.
Do not run an empty rotor. Place filled tubes in at least two opposing cavities. Make sure that filled
containers are loaded symmetrically into the rotor and that opposing tubes are filled to the same
level with liquid of the same density.
If disassembly reveals evidence of leakage, you should assume that some fluid escaped the rotor.
Apply appropriate decontamination procedures to the centrifuge and accessories.
Never exceed the maximum rated speed of the rotor and labware in use. Refer to the section on Run
Speeds, and derate the run speed as appropriate.
Do not use sharp tools on the rotor that could cause scratches in the rotor surface. Corrosion begins
in scratches and may open fissures in the rotor with continued use.

v
Safety Notice,iii
Alerts for Warning, Caution, Important, and Note,iii
Safety Information for the JA-25.50 Rotor, iii
JA-25.50
Fixed-Angle Rotor,1
Specifications,1
Description,2
Preparation and Use,4
Care and Maintenance,14
Returning a Rotor,18
Supply List,19
Beckman Coulter, Inc.
J Series Rotor Warranty
Related Documents

vi
1TheJA-25.50Fixed-AngleRotor,3
2 Fluid-Containment Annulus and Finger-Grip Grooves,4
3 Typical Examples of Arranging Tubes in the Rotor,5

vii
1 Temperature Compensation Settings for Analog- and Microprocessor-
Controlled Centrifuges ,8
2 Temperature Compensation Settings for the Model J2-HC Analog Centrifuge.
,8
3 Available Tubes and Bottles for the JA-25.50 Rotor,12
4 Relative Centrifugal Fields for the JA-25.50 Rotor,15

PN J-TB-056FD 1
JA-25.50
Fixed-Angle Rotor
Specifications
1. Axis of Rotation
Maximum speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25,000 RPMa
Critical speed rangeb. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 600 to 800 RPM
Density rating at maximum speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2 g/mL
Maximum allowable imbalance of opposing loads . . . . . . . . . . . . . . . . . . . . . . . . 2 grams
Relative Centrifugal Fieldcat maximum speed
At rmax (108 mm). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75,6000 g
At rav (73.2 mm). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51,200 g
At rmin (38.5 mm) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27,000 g
kfactor at maximum speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 418
Conditions requiring speed reductions. . . . . . . . . . . . . . . . . . . . . . . . . . . see
Run Speeds
Number of tube cavities (round bottom) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Available bottles and tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . see
Table 3
Nominal tube dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 104 mm
Nominal tube capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 mL
Nominal rotor capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 400 mL
Approximate acceleration time to maximum speed (rotor fully loaded)
in an Avanti J centrifuge (to 25,000 RPM) . . . . . . . . . . . . . . . . . . . . . . . 3 1/
2 min
in a J2 series centrifuge (to 21,000 RPM . . . . . . . . . . . . . . . . . . . . . . . . 1 3/
4 min
Approximate deceleration time from maximum speed
(rotor fully loaded, max. brake)
in an Avanti J centrifuge (from 25,000 RPM) . . . . . . . . . . . . . . . . . . . . 1 3/
4 min
in a J2 series centrifuge (from 21,000 RPM) . . . . . . . . . . . . . . . . . . . . . 2 1/
2 min
Rotor weight, empty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8.1 kg (17.8 lb)
Rotor weight, maximum sample mass. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8.7 kg (19.1 lb)
Rotor material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . aluminum
Rotor entry code for microprocessor-controlled J2 series
instruments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25.5
rmin
rav
rmax
34°
1

PN J-TB-056FD
2
JA-25.50 Fixed-Angle Rotor
Description
Description
Beckman Coulter JA-25.50 rotors are manufactured in a facility that maintains certifications to both
ISO 9001:2008 and ISO 13485:2003. They are for use with the specified Beckman Coulter centrifuges.
The JA-25.50 fixed-angle rotor is designed to hold up to eight 50-mL tubes at a 34-degree angle (see
Figure 1). Used in Beckman Coulter Avanti J and J2 series centrifuges, the JA-25.50 rotor develops
centrifugal forces that can efficiently pellet cells from large volumes, or cell particles from tissue
homogenates. Short column methods (i.e., partially filled tubes) may also be used to purify large
quantities of virus in a cushion gradient.
a. Discontinued Avanti J-25I and Avanti J-25, 50-Hz models: at 4°C (at 24°C ambient) – 24,000 RPM (69,700 g) at 7°C (at 24°C ambient) –
25,000 RPM (75,600 g). In Avanti JXN-26, J-26S XP series, J-26 XP series, and discontinued J-20 XP series, at speeds above 20,000, the
minimum temperatrue set point will be above 4°C. NOTE: at ambient temperatures above 24°C, the temperatures listed above may not be
achievable.
b. The critical speed range is the range of speeds over which the rotor shifts so as to rotate about its center of mass. Passing through the critical
speed range is characterized by some vibration.
c. Relative Centrifugal Field (RCF) is the ratio of the centrifugal acceleration at a specified radius and speed (r
2
) to the standard acceleration of
gravity (g) according to the following formula: RCF = r

/g — where ris the radius in millimeters, is the angular velocity in radians per second
(2 RPM /60), and gis the standard acceleration of gravity (9807 mm/s
2
). After substitution: RCF = 1.12r(RPM/1000)
2
1. Daisy Knob
2. Lid
3. Small O-ring (011757)
4. Large O-ring (870612)
5. Rotor Body
6. Dual Locking Lid Shown
1
5
4
3
2
6

PN J-TB-056FD 3
JA-25.50 Fixed-Angle Rotor
Description
Figure 1 The JA-25.50 Fixed-Angle Rotor*
The dual-locking lid assembly allows the rotor to be loaded into and removed from the centrifuge
with the lid attached. The rotor may be placed under a safety hood before the lid is attached or
removed. The rotor is also available with a single-locking lid assembly. The rotor has been tested
†
with both lid configurations to demonstrate containment of microbiological aerosols under normal
conditions of the associated Beckman Coulter centrifuge when used and maintained as instructed.
The JA-25.50 rotor has a patented fluid-containment annulus, located below the O-ring sealing
surface (see Figure 2). If tubes are overfilled or if leakage occurs during centrifugation, the annulus
holds enough volume that all of the liquid is kept inside the rotor—even if all eight tubes leak at the
same time. This feature virtually eliminates the escape of liquid into the centrifuge chamber.
Finger-grip grooves in the area around and between each tube cavity (see Figure 2) provide extra
room for fingers as tubes are loaded and unloaded.
The rotor and lid are made of anodized aluminum. Two O-rings made of Buna N rubber, both located
in the rotor lid, help to maintain atmospheric pressure inside the rotor during centrifugation when
they are properly lubricated. Vertical pins in the rotor drive hole mate with the centrifuge drive
hub to prevent the rotor from slipping during acceleration and deceleration.
See the Warranty at the back of this manual for warranty information.
* The dual-llocking lid is shown,.
† Validation of microbiological containment was done at an independent third-party testing facility (CAMR, Porton Down, UK,
or USAMRIID, Ft. Detrick, MD, U.S.A.). Improper use or maintenance may affect seal integrity and thus containment.
Rotor
Labware
Lid

PN J-TB-056FD
4
JA-25.50 Fixed-Angle Rotor
Preparation and Use
Figure 2 Fluid-Containment Annulus and Finger-Grip Grooves
Preparation and Use
Specific information about the JA-25.50 rotor is given here. Use the Rotors and Tubes for
Beckman Coulter J Series Centrifuges manual (publication JR-IM) and the centrifuge instruction
manual along with this manual for complete rotor and accessory operation.
NOTE
Although rotor components and accessories made by other manufacturers may fit in the JA-25.50
rotor, their safety in this rotor cannot be ascertained by Beckman Coulter. Use of other manufacturers’
components or accessories in the JA-25.50 rotor may void the rotor warranty and should be prohibited by
your laboratory safety officer. Only the components and accessories listed in this publication should be
used in this rotor.
Prerun Safety Checks
Read the Safety Notice section at the front of this manual before using the rotor.
1 Make sure that the rotor, lid, and all tubes or bottles and accessories are clean and show no signs
of corrosion or cracking.
2 Verify that the bottles or tubes being used are listed in Table 3.
3 Refer to Chemical Resistances (publication IN-175), included in the Rotors and Tubes CDsfor the
chemical compatibilities of all materials used.
1. Fluid Containment Annulus
2. O-ring Sealing Surface
3. Finger-Grip Grooves
1
3
2

PN J-TB-056FD 5
JA-25.50 Fixed-Angle Rotor
Preparation and Use
Rotor Preparation
For runs at other than room temperature refrigerate or warm the rotor beforehand for fast equilibration.
1 Be sure that metal threads in the rotor are clean and lightly but evenly lubricated with Spinkote
lubricant (306812).
Also ensure that O-rings are lightly but evenly coated with silicone vacuum grease (335148).
2 Load the filled containers symmetrically into the rotor.
• (Refer to Tubes and Bottles on page 11 for information about containers.)
• If fewer than eight tubes are being run they must be arranged symmetrically in the rotor
(see Figure 3).
• Opposing tubes must be filled to the same level with liquid of the same density.
Figure 3 Typical Examples of Arranging Tubes in the Rotor
NOTE Two, four, six, or eight tubes can be centrifuged per run if they are arranged in the rotor as shown.
NOTE Do not run an empty rotor. Place filled tubes in at least two opposing cavities.
Operation
1 Precool the rotor in the centrifuge or in a refrigerator before use—especially before short
runs—to ensure that the rotor reaches the set temperature.
A suggested precooling cycle is a minimum of 30 minutes at 2000 RPM at the required
temperature.
2 If fluid containment is required, use capped tubes or bottles.
It is strongly recommended that all containers carrying physiological fluids be capped to
prevent leakage.
3 If you are using an Avanti J series centrifuge, select the JA-25.50 rotor.

PN J-TB-056FD
6
JA-25.50 Fixed-Angle Rotor
Preparation and Use
4 If you are using a microprocessor-controlled J2 series centrifuge, enter rotor code 25.5 .
Refer to your centrifuge instruction manual for additional information.
5 For additional operating information, see the following:
• Run Times, page 13, for using kfactors to adjust run durations.
• Run Speeds, page 14, for information about speed limitations.
Installing the Rotor
1 To prevent the rotor from sticking to the centrifuge drive hub, apply a thin coat of Spinkote
lubricant to the rotor drive pins and to the lid knob threads
2 Lightly coat the large and small lid O-rings with silicone vacuum grease.
3 Dual-locking lid only. Place filled tubes into the rotor, then place the lid on the rotor and turn the
daisy knob to the right (clockwise) until secure.
Do not overtighten.
4 Carefully lower the rotor straight down onto the centrifuge drive spindle hub.
Do not drop the rotor onto the spindle hub.
5 Slowly turn the rotor around the drive spindle to make sure that it is properly seated.
6 Secure the rotor to the centrifuge drive spindle as follows:
a. Dual-locking lid: press the lid knob down and turn it to the right (clockwise) until secure,
no more than two full turns.
Do not overtighten

PN J-TB-056FD 7
JA-25.50 Fixed-Angle Rotor
Preparation and Use
b. Single-locking lid: place filled tubes into the rotor, then place the lid on the rotor.
Press the lid knob down and turn it to the right (clockwise) until secure.
Do not overtighten.
CAUTION
If the rotor is left in the centrifuge between runs, make sure that the rotor is
seated on the drive hub and that the tie-down knob is tight before each run.
Temperature
To ensure that the JA-25.50 rotor reaches the required temperature during the run, follow the
appropriate instructions below for the model of J centrifuge being used.
Avanti J Series Centrifuges
Enter the run temperature according to the instructions in your centrifuge instruction manual.
No additional input is required.
1. Tie-down Knob
2. Daisy Knob
3. Lubricate Threads
4. Dual-Locking Lid
1. Tie-down Kknob
2. Lubricate Threads
3. Single-Locking Lid
1
4
3
2
1
3
2

PN J-TB-056FD
8
JA-25.50 Fixed-Angle Rotor
Preparation and Use
J2 Series Centrifuges
Enter the required run temperature and the appropriate temperature compensation units (see
Table 1 and Table 2) on the centrifuge control panel as follows.
Analog J2 Series Centrifuges (Models J2-HS and J2-HC)
1 Turn the SET knob on the centrifuge control panel to the required sample temperature.
2 Find the compensation value in Table 1 for Models J2-HS or Table 2 for Model J2-HC that
corresponds to the required temperature and run speed.
Set the
COMP dial to that setting.
(Interpolate if intermediate values are required.)
NOTE
Temperature settings for J-21 and J2-21 series centrifuges must be empirically determined.
Table 1 Temperature Compensation Settings for Analog- and Microprocessor-Controlled Centrifuges a
a. (Models J2-HS, J2-MI, and J2-MC). Interpolate if intermediate values are required.
Rotor
Speed
(RPM)
Required Sample Temperature (°C)
–20 –10 2 5 10 20 40
21,000 Nb
b. An “N” indicates that the rotor cannot achieve the required temperature at this speed.
N –7–5–6–5–9
18,000N –5–5–5–5–4–7
15,0000 –2–3–3–2–2–6
10,000 0 0 –1 0 –1 0 –3
Table 2 Temperature Compensation Settings for the Model J2-HC Analog Centrifuge. a
a. Interpolate if intermediate values are required
Rotor
Speed
(RPM)
Required Sample Temperature (°C)
–20 –10 2 5 10 20 40
18,000 Nb
b. An “N” indicates that the rotor cannot achieve the required temperature at this speed.
–8 –6 –6 –6 –6 –5
15,000 –7 –6 –5 –5 –5 –4 –4
10,000 –5 –3 –2 –3 –4 –3 –2

PN J-TB-056FD 9
JA-25.50 Fixed-Angle Rotor
Preparation and Use
Microprocessor-Controlled J2 Series Centrifuges (Models J2-MI and J2-MC)
1 Press the TEMP key on the centrifuge control panel and then use the keypad to enter the sample
temperature.
2 Find the compensation value in Table 1 that corresponds with the set temperature and
run speed.
3 Press COMP ADJ .
The word “
COMP ” flashes below the TEMPERATURE display and the display flashes.
4 Use the keypad to enter the required compensation value.
Press the .key to enter a minus sign; pressing it again will remove the minus sign.
5 Check the temperature display. (If the entry is incorrect, press ce and reenter the digits.)
6 When the entry is correct, press ENTER/RECALL .
NOTE
To clear a COMP ADJ entry, press COMP ADJ, 0, and ENTER/RECALL.
Run Procedure
When the rotor is properly loaded and installed in the centrifuge, you are ready to perform the run.
Refer to the applicable centrifuge instruction manual for operating instructions.

PN J-TB-056FD
10
JA-25.50 Fixed-Angle Rotor
Preparation and Use
WARNING
Operator error or tube failure may generate aerosols. Toxic, pathogenic, or other
hazardous materials must not be run in this rotor unless all appropriate safety
precautions are taken. Also see the Safety Notice at the beginning of this manual.
CAUTION
Make sure that the rotor is properly seated on and securely fastened to the drive
hub before each run.
Removal and Sample Recovery
CAUTION
If disassembly reveals evidence of leakage, you should assume that some fluid
escaped the rotor. Apply all appropriate safety and decontamination procedures
to the centrifuge and accessories.
Dual-Locking Lid
1 Place one hand on the rotor to keep it from turning.
Grasp the round lid knob with your other hand and turn the knob to the left (counterclockwise)
until the stem disengages from the drive hub threads.
2 Hold the knob or place your hands on the rotor and lift the rotor straight up off the drive hub.
3 Place the rotor on a counter or bench top under a safety hood.
4 Grasp the daisy knob and turn it to the left (counterclockwise) until it disengages.
5 Remove the lid and set it aside.

PN J-TB-056FD 11
JA-25.50 Fixed-Angle Rotor
Preparation and Use
Single-Locking Lid
1 Unscrew the lid knob.
2 Remove the lid and set it aside.
3 Lift the rotor straight up off the drive hub to avoid bending or damaging the hub.
Tubes and Bottles
The JA-25.50 rotor uses the tubes and bottles listed in Table 3. Be sure to use only those items listed,
and to observe the maximum speed limits and fill volumes shown. (Maximum fill volume is the
maximum amount that can be centrifuged in the container listed.)
Refer to Chemical Resistances (publication IN-175) for information on the chemical compatibilities
of tube and accessory materials.
Temperature Limits
• Plastic tubes and bottles have been centrifuge tested for use at temperatures between 2 and
25°C. For centrifugation at other temperatures, pretest tubes under anticipated run conditions.
• If plastic containers are frozen before use, make sure that they are thawed to at least 2°C prior
to centrifugation.
Thickwall Tubes
Thickwall polypropylene, and polycarbonate tubes can be run partially filled (at least half filled)
with or without caps, but all opposing tubes for a run must be filled to the same level with liquid of
the same density. Do not overfill capless tubes..

PN J-TB-056FD
12
JA-25.50 Fixed-Angle Rotor
Preparation and Use
Table 3 Available Tubes and Bottles for the JA-25.50 Rotora
Tube or Bottle Required Accessory
Part
Number
Max
Speedb/
RCF/
kfactor
Dimensions
and Volume Description
Part
Number
Max. Fill
Volume
(mL) Description
24 104 mm
50 mL
polycarbonate bottle
assy w/cap
361693 40 none — 25,000 RPM
75,600 g
418
29 104 mm
50 mL
polypropylene bottle
assy w/cap
361694 40 none — 25,000 RPM
75,600 g
418
29 104 mm
50 mL
polycarbonate bottle,
screw-cap
357002 40 none — 25,000 RPM
75,600 g
418
29 104 mm
50 mL
polypropylene bottle,
screw-cap
357003 40 none — 25,000 RPM
75,600 g
418
29 104 mm
50 mL
polycarbonate thickwall
tube, snap-on cap
363664 36.5 natural 29-mm cap
red 29-mm cap
green 29-mm cap
yellow 29-mm cap
blue 29-mm cap
356264c
357359c
357360c
357361c
357362c
20,000 RPM
48,400 g
653
9 104 mm
50 mL
polypropylene
thickwall tube,d
snap-on cap
357005 36.5 natural 29-mm cap
red 29-mm cap
green 29-mm cap
yellow 29-mm cap
blue 29-mm cap
356264c
357359c
357360c
357361c
357362c
20,000 RPM
48,400 g
653
29 104 mm
50 mL
polycarbonate thickwall
tube, no cap
363647 35 none — 25,000 RPM
75,600 g
418
29 104 mm
50 mL
polypropylene thickwall
tube, no cap
357007 35 none — 25,000 RPM
75,600 g
418
16 80 mm
10 mL
polycarbonate bottle,
screw-cap
355672 10 adapter 361703e25,000 RPM
63,240 g
350
9.5 38 mm
1.5 mL
polypropylene tube 357448 1.3 adapter 361705e25,000 RPM
50,960 g
182

PN J-TB-056FD 13
JA-25.50 Fixed-Angle Rotor
Preparation and Use
Polycarbonate and Polypropylene Bottles
Capped polycarbonate and polypropylene bottles may be centrifuged completely filled, or partially
filled (not less than half full). Containers placed in opposing positions in the rotor must be filled
to the same level..
Run Times
To pellet particles of known sedimentation coefficient s, calculate the run time tusing the kfactor
(395) of the rotor.
*
Time is in hours and sis in Svedberg units.
EQ 1
For example, at 25,000 RPM, mammalian viruses (s= 700 S) can be pelleted in
EQ 2
9.5 38 mm
1.5 mL
polypropylene tube 356090 1.3 adapter 361705e25,000 RPM
50,960 g
182
25 105 mm glass tubeff f adapter 870331 f
a. Use only the items listed here and observe fill volumes and maximum run speeds.
b. Maximum speeds listed are guidelines only. These speeds have been achieved in reliability tests at Beckman Coulter, but because of
manufacturing variances no guarantee of performance or fit is expressed or implied.
c. Polyethylene, package of 25.
d. Above 20°C fill polypropylene tubes at least half full. Containers may be filled less than or equal to the maximum fill volumes provided in
this table.
e. Polyetherimide (PEI); package of 2.
f. Commercially available. Observe manufacturer’s speed and fill volume guidelines.
Table 3 Available Tubes and Bottles for the JA-25.50 Rotora(Continued)
Tube or Bottle Required Accessory
Part
Number
Max
Speedb/
RCF/
kfactor
Dimensions
and Volume Description
Part
Number
Max. Fill
Volume
(mL) Description
* Based on sedimentation in water at 20°C. Assumes full tubes.
tk
s
---25 000
actual run speed
----------------------------------------


2
=
t395
700
---------25 000
25 000
----------------


2
30 minutes.≈=