Beckman Coulter Avanti J-25 User manual

Instruction Manual
J325I-IM-7
®
®
High-Performance Centrifuge
Avanti® J-25

Symbol
Symbol
Symbole
Símbolo
Title / Titel / Titre / Titulo / Titolo /
Dangerous voltage
Gefährliche elektrische Spannung
Courant haute tension
Voltaje peligroso
Pericolo: alta tensione
Attention, consult accompanying documents
Achtung! Begleitpapiere beachten!
Attention, consulter les documents joints
Atención, consulte los documentos adjuntos
Attenzione: consultare le informazioni allegate
On (power)
Ein (Netzverbindung)
Marche (mise sous tension)
Encendido
Acceso (sotto tensione)
Off (power)
Aus (Netzverbindung)
Arrêt (mise hors tension)
Apagado
Spento (fuori tensione)
Protective earth (ground)
Schutzleiteranschluß
Liaison à la terre
Puesta a tierra de protección
Collegamento di protezione a terra
Earth (ground)
Erde
Terre
Tierra
Scarica a terra
!
/
Simbolo

SAFETY NOTICE
This safety notice summarizes information basic to the safe operation of the equipment
described in this manual. The international symbol displayed above is a reminder that all
safety instructions should be read and understood before installation, operation, mainte-
nance, or repair of this instrument. When you see the symbol on other pages, pay special
attention to the safety information presented. Observance of safety precautions will also
help to avoid actions that could damage or adversely affect the performance of the
centrifuge.
Safety During Installation and/or Maintenance
This centrifuge is designed to be installed by a Beckman Coulter field service representa-
tive. Installation by anyone other than authorized Beckman Coulter personnel invalidates
any warranty covering the instrument.
Any servicing of this equipment that requires removal of any covers can expose parts
which involve the risk of electric shock or personal injury. Make sure that the power
switch is turned off and the instrument is disconnected from the main power source,
and refer such servicing to qualified personnel.
Be sure to use the anchoring system to secure the centrifuge in place. The anchoring
system is designed to reduce the possibility of injury or damage which could result from
instrument movement in the event of a major rotor mishap.
Electrical Safety
To reduce the risk of electrical shock, this equipment uses a three or five-wire electrical
cord and plug to connect this equipment to earth-ground. To preserve this safety feature:
• Make sure that the matching wall outlet receptacle is properly wired and earth-
grounded. Check that the line voltage agrees with the voltage listed on the name-rating
plate affixed to the centrifuge.
• Never use a three-to-two wire plug adapter.
• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-
outlet receptacle strip.
Do not place containers holding liquid on or near the chamber door. If they spill, liquid
may get into the centrifuge and damage electrical or mechanical components.
Safety Against Risk of Fire
This centrifuge is not designed for use with materials capable of developing flammable
or explosive vapors. Do not centrifuge such materials (for example, chloroform or ethyl
alcohol) in this instrument nor handle or store them near the centrifuge.
!

Mechanical Safety
For safe operation of the equipment, observe the following:
• Use only the Beckman Coulter rotors and accessories designed for use in this
centrifuge.
• Do not exceed the maximum rated speed of the rotor in use.
• NEVER attempt to slow or stop a rotor by hand.
• Do not lift or move the centrifuge while a rotor is spinning.
• NEVER attempt to override the door interlock system while the rotor is spinning.
• Do not lean on the centrifuge or place items on it while it is operating.
Chemical and Biological Safety
Normal operation may involve the use of solutions and test samples that are pathogenic,
toxic, or radioactive. Such materials should not be used in this centrifuge, however, unless
all necessary safety precautions are taken.
• Observe all cautionary information printed on the original solution containers prior to
their use.
• Handle body fluids with care because they can transmit disease. No known test offers
complete assurance that these fluids are free of micro-organisms. Some of the most
virulent—Hepatitis (B and C) viruses and HIV (I–V), atypical mycobacteria, and
certain systemic fungi—further emphasize the need for aerosol protection. Handle
other infectious samples according to good laboratory procedures and methods to
prevent spread of disease. Because spills may generate aerosols, observe proper safety
precautions for aerosol containment. Do not run toxic, pathogenic, or radioactive
materials in this centrifuge without taking appropriate safety precautions. Biosafe
containment should be used when Risk Group II materials (as identified in the World
Health Organization Laboratory Biosafety Manual) are handled; materials of a higher
group require more than one level of protection.
• Dispose of all waste solutions according to appropriate environmental health and safety
guidelines.
It is your responsibility to decontaminate the instrument and accessories before
requesting service by Beckman Coulter Field Service.

J325I-IM-7
January 2007
Instruction Manual
®
High-Performance Centrifuge
© 2007 Beckman Coulter, Inc.
Printed in the U.S.A.
JUC-0107-PDF
Avanti®J-25

Contents
iii
Page
INTRODUCTION
Certification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Scope of this Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . viii
Radio Interference . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
Recycling Label . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x
SECTION 1: SPECIFICATIONS AND PREINSTALLATION REQUIREMENTS
Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Control Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Operational Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Physical Data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Available Rotors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Preinstallation Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-7
Electrical Requirements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-7
Space and Location Requirements. . . . . . . . . . . . . . . . . . . . . . . . 1-10
Securing the Centrifuge to the Floor . . . . . . . . . . . . . . . . . . . . . . .1-12
Using J2 Series Rotors in the Avanti J. . . . . . . . . . . . . . . . . . . . . . . . 1-12
Checking for Rotor Drive Pins . . . . . . . . . . . . . . . . . . . . . . . . . . 1-13
Using the JA-18 Rotor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-14
Using the JCF-Z Continuous Flow/Zonal Rotor . . . . . . . . . . . . . 1-14
SECTION 2: DESCRIPTION
Centrifuge Function and Safety Features. . . . . . . . . . . . . . . . . . . . . . . 2-1
Centrifuge Function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
Safety Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Housing and Door . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
Rotor Chamber. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
Drive . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
Friction Reduction System (FRS) . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
Temperature Sensing and Control. . . . . . . . . . . . . . . . . . . . . . . . . 2-4
Overtemp System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
Name Rating Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
Controls and Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
Power Switch and Key Switches. . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
Control Panel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6
Keypad. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-10
System Keys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-11

iv
Contents
Page
SECTION 3: OPERATION
Summary of Run Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Manual Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Programmed Run. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
Installing the Rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
Manual Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
Selecting a Rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
Entering Run Speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
Entering Run Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-7
Entering Run Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10
Entering Acceleration and Deceleration Rates . . . . . . . . . . . . . . 3-11
Starting a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-13
Changing Parameters During a Run . . . . . . . . . . . . . . . . . . . . . . 3-13
Ending a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-13
Programmed Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-14
Creating a New Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-14
Modifying a Program. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-16
Running a Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-17
Setting Up a Zonal Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-17
Setting Up an Elutriation Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-19
SECTION 4: TROUBLESHOOTING
User Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
Accessing the Rotor in Case of Power Failure. . . . . . . . . . . . . . . . . . . 4-5
JCF-Z Rotor Identification. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-10
SECTION 5: CARE AND MAINTENANCE
Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
Cleaning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
Sterilization and Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
Replacing the Air Filter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Circuit Breaker and Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Storage and Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Storage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Returning a Centrifuge. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Supply List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-6
Replacement Parts and Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . 5-6
APPENDIX: TEMPERATURE CALIBRATION PROCEDURE
WARRANTY

Illustrations
v
Tables
Page
Figure 1-1. Single-Phase Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . 1-9
Figure 1-2. Three-Phase “Y” Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . 1-9
Figure 1-3. Correct and Incorrect Three-Phase Power Configurations. . . . . . . . . . 1-10
Figure 1-4. Rear View and Dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-11
Figure 1-5. Checking the Rotor for Drive Pins . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-13
Figure 2-1. Power and Key Switches . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
Figure 2-2. The Control Panel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7
Figure 2-3. The Actual Value Display. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7
Figure 2-4. The Setup Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-8
Figure 3-1. Elutriation Controls. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-19
Figure 4-1. Emergency Door Release Latch Access. . . . . . . . . . . . . . . . . . . . . . . . . 4-6
Figure 4-2. Manual Door Release . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
Figure 4-3. Venting the Chamber Vacuum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-9
Figure 4-4. Front Panel Retaining Strip. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-10
Page
Table 1-1. Nominal Instrument Voltage Ratings . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8
Table 1-2. Required Wire Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-9
Table 3-1. Acceleration and Deceleration Settings . . . . . . . . . . . . . . . . . . . . . . . . .3-11
Table 4-1. Diagnostic Message Chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2

Introduction
vii
CERTIFICATION
To ensure full system quality, Beckman Coulter Avanti®J-25 I
centrifuges are manufactured in a registered ISO 9001 facility. They
have been designed and tested to be compliant (when used with
Beckman Coulter rotors) with the laboratory equipment requirements
of applicable regulatory agencies. Declarations of conformity and
certificates of compliance are available at www.beckmancoulter.com.
SCOPE OF THIS MANUAL
This manual is designed to familiarize you with the Avanti®J-25I
centrifuge, its functions, specifications, operation, and routine
operator care and maintenance. We recommend that you read this
entire manual, especially the SAFETY NOTICE and all safety-related
information, before operating the instrument or performing instru-
ment maintenance.
• Section 1 contains system specifications and instructions for
preparing your site for installation of the centrifuge.
• Section 2 contains an overall description of the centrifuge,
including a description of system controls and indicators.
• Section 3 summarizes procedures for operating the centrifuge.
• Section 4 lists possible error messages and/or malfunctions,
together with probable causes and required corrective actions.
• Section 5 contains procedures for routine maintenance, as well as
a brief list of supplies and replacement parts.
• The Appendix contains a procedure to use when temperature
control within ±1°C is required.

viii
Introduction
➠NOTE
If the centrifuge is used in a manner other than
specified in this manual, the safety and perfor-
mance of this equipment could be impaired.
Further, the use of any equipment other than
that recommended by Beckman Coulter has not
been evaluated for safety. Use of any equipment
not specifically recommended in this manual is
the sole responsibility of the user.
CONVENTIONS
Certain symbols are used in this manual to call out safety related and
other important information. These international symbols may also be
displayed on the centrifuge and are reproduced and described below
and on the inside of the front cover.
NOTES, CAUTIONS, AND WARNINGS
➠NOTE
Used to call attention to information that should
be followed during installation, use, and/or
servicing of the equipment.
!CAUTION
Used to indicate a potentially hazardous situa-
tion which, if not avoided, may result in minor or
moderate injury and/or mechanical damage. It
is also used to alert against unsafe practices.

ix
Introduction
WARNING
!Used whenever an action or condition may
potentially cause serious personal injury or
loss of life. Mechanical damage may also
result.
WARNING
Indicates high voltage or risk of electric
shock. Refer servicing of all areas displaying
this symbol to service personnel.
TYPOGRAPHIC CONVENTIONS
Certain typographic conventions are used throughout this manual to
distinguish names of user interface components, such as keys and
displays.
•Key names (for example, [START] and [STOP]) appear in capital
letters within brackets.
• Actual value display names and setup screen field names
(for example, TEMP°C and Rotor:) appear in bold type.
RADIO INTERFERENCE
This instrument has been tested and found to comply with the limits
for a Class A digital device, pursuant to Part 15 of FCC Rules. These
limits are designed to provide reasonable protection against harmful
interference when the equipment is operated in a commercial
environment. This equipment generates, uses, and can radiate radio
frequency energy and, if not installed and used in accordance with
this instruction manual, may cause interference to radio communica-
tions. Operation of this equipment in a residential area may cause
interference, in which case the user will be required to correct the
interference at his own expense.

x
Introduction
CANADIAN REGULATIONS
This digital apparatus does not exceed the Class A limits for radio
noise emissions from digital apparatus as set out in the radio interfer-
ence regulations of the Canadian Department of Communications.
Le présent appareil numérique n’émet pas de bruits radioélectriques
dépassant les limites applicables aux appareils numériques de Classe
A prescrites dans le reglement sur le brouillage radioelectrique édicté
par le Ministère des Communications du Canada.
RECYCLING LABEL
This symbol is required in accordance with the Waste Electrical and
Electronic Equipment (WEEE) Directive of the European Union. The
presence of this marking on the product indicates:
1) the device was put on the European market after August 23, 2005
and
2) the device is not to be disposed via the municipal waste collection
system of any member state of the European Union.
It is very important that customers understand and follow all laws
regarding the proper decontamination and safe disposal of electrical
equipment. For Beckman Coulter products bearing this label please
contact your dealer or local Beckman Coulter office for details on the
take back program that will facilitate the proper collection, treatment,
recovery, recycling and safe disposal of the device.
A28219-AA
Note: On the instrument, the
triangle background is yellow
rather than gray.

1
Specifications and
Preinstallation Requirements
1-1
SPECIFICATIONS Only values with tolerances or limits are guaranteed data. Values
without tolerances are informative data, without guarantee.
CONTROL FEATURES
Speed
Setting range . . . . . . . . . . . . . .0 to 25 000 rpm (in 10-rpm increments)
or equivalent RCF (in 100 ×gincrements)
Elutriation speed setting range. . . . . . . . . . . 0 to 6 000 rpm (in 10-rpm
or 10 ×gincrements)
Speed display . . . . . . . . . . . . . . . . . . . . from 0 to 10 000 rpm, indicates actual
rotor speed ±10 rpm
from 10 000 to 25 000 rpm, indicates actual
rotor speed ±0.1%, or equivalent rcf
Time
Setting range . . . . . . .1 min to 99 hr 59 min,
ω
2t, or continuous (hold)
Actual display . . . . . . . . . . . indicates run time remaining (timed run),
ω
2t, or elapsed time (hold run)
ω
2tsetting range . . . . . . up to 9.99 ×1014 radians squared per second
ω
2tactual display . . . . . . . indicates accumulated centrifugal effect to
3 significant digits (in exponential notation)
Temperature
Setting range . . . . . . . . . . . . . . . . . . –20 to +40°C (in 1°C increments)
Accuracy . . . . . . . . . . . . . . . . . . rotor temperature controlled to within
±2°C of set temperature (after equilibration)*
Ambient temperature range . . . . . . . . . . . . . 16 to 38°C (60 to 100°F)†
Cooling fluid . . . . . . . . . . . . . . . . . . . . . . . . . Refrigerant 404A (HFC)
Acceleration . . . . . . . . . . . . . . . . . . . . . . . . . . . . .maximum, slow, or timed
(1 to 10 min from 0 to 500 rpm)
Deceleration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . maximum,slow, timed
(1 to 10 min from 500 to 0 rpm), or off
*During transient conditions, such as acceleration and deceleration, rotor temperature may be
outside this range.
†To reach temperatures above ambient, the centrifuge is dependent on the frictional heat
generated inside the chamber during operation. At low run speeds or low ambient tempera-
tures, the centrifuge may not be able to achieve some higher temperatures.
CFC

1-2
Specifications and Preinstallation Requirements
OPERATIONAL FEATURES
Door. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.4-cm (2.1-in.) thick structural
foam with steel plate
Rotor chamber diameter . . . . . . . . . . . . . . . . . . . . . . . . . . . 40.6 cm (16 in.)
Friction Reduction System (FRS) . . . . . . . .A mechanical rotary vane pump
reduces chamber pressure to one-quarter
atmosphere (190 mm [7.5 in.] Hg)
PHYSICAL DATA
Width. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71 cm (28 in.)
Depth. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86 cm (34 in.)
Depth (including air diverter extending
from back panel) . . . . . . . . . . . . . . . . . . . . . . . . . . . 102 cm (40.25 in.)
Height, door closed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86 cm (34 in.)
Height to top of control head. . . . . . . . . . . . . . . . . . . . . . . 116 cm (45.5 in.)
Height to top of open door . . . . . . . . . . . . . . . . . . . . . . . . 141 cm (55.5 in.)
Weight . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 264 kg (583 lb)
Clearances (for adequate ventilation)
Sides. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.6 cm (3 in.)
Back (place air diverter against the wall). . . . . . . . . . . 16 cm (6.25 in.)
Surface finish. . . . . . . . . . . . . . . polyester control panel with polycarbonate
coating overlay; polyurethane enamel on door and covers;
vinyl paint on other surfaces
Maximum heat dissipation into room
under steady-state conditions. . . . . . . . . . . . . . . . 6900 Btu/h (2.0 kW)
Humidity restrictions. . . . . . . . . . . . . . . . . . . . . . . . <95% (noncondensing)
Noise level 0.91 m (3 ft) in front of instrument
at 25 000 rpm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57 dBa
Installation category . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . II
Pollution degree. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

1-3
Specifications and Preinstallation Requirements
AVAILABLE ROTORS
Refer to the applicable rotor manual for complete rotor descriptions.
aMaximum speeds are based on a solution density of 1.2 g/mL with the following exceptions: the JA-18.1 rotor is rated for a
density of 1.4 g/mL; the JCF-Z rotor is rated for a density of 1.45 g/mL; and the JE-6B rotor is rated for a density of 3 g/mL.
bMaximum speed for the JA-30.50 Ti rotor is 25 000 rpm in the Avanti J-25I centrifuge, and 30 000 rpm in the Avanti J-30I centrifuge.
cMaximum speed for the JS-24.38 and JS-24.15 rotors is 10 000 rpm in the Avanti J-25I centrifuge, and 24 000 rpm in the
Avanti J-30I centrifuge.
Rotor Profile and Description Max
RPMaMax RCF
(×g)Max Capacity
Rotor
Manual
Number
JA-30.50 Ti Fixed Angle, 34° (8 place)
rmax = 108 mm 25 000b75 600 8 ×50 mL J-TB-070
JA-25.50 Fixed Angle, 34° (8 place)
rmax = 108 mm 25 000 75 600 8 ×50 mL J-TB-056
JA-25.15 Fixed Angle, 25° (24 place)
rmax = 106 mm
(outer row)
rmax = 86 mm
(inner row)
25 000 74 200
(outer row)
60 200
(inner row)
24 ×15 mL J-TB-057
JA-21 Fixed Angle, 40° (18 place)
rmax = 102 mm 21 000 50 400 18 ×10 mL J-TB-002
JA-20.1 Fixed Angle, 23° (32 place)
rmax = 115 mm
(outer row)
rmax = 98 mm
(inner row)
20 000 51 500
(outer row)
43 900
(inner row)
32 ×15 mL J-TB-022
—Continued

1-4
Specifications and Preinstallation Requirements
aMaximum speeds are based on a solution density of 1.2 g/mL with the following exceptions: the JA-18.1 rotor is rated for a
density of 1.4 g/mL; the JCF-Z rotor is rated for a density of 1.45 g/mL; and the JE-6B rotor is rated for a density of 3 g/mL.
bMaximum speed for the JA-30.50 Ti rotor is 25 000 rpm in the Avanti J-25I centrifuge, and 30 000 rpm in the Avanti J-30I centrifuge.
cMaximum speed for the JS-24.38 and JS-24.15 rotors is 10 000 rpm in the Avanti J-25I centrifuge, and 24 000 rpm in the
Avanti J-30I centrifuge.
Rotor Profile and Description Max RPMa
Max
RCF
(×g)Max Capacity
Rotor
Manual
Number
JA-20 Fixed Angle, 34° (8 place) rmax = 108 mm 20 000 48 400 8 ×50 mL J-TB-003
JA-18.1 Fixed Angle (24 place)
45° adapter
rmax = 116 mm
25° adapter
rmax = 112 mm
18 000
17 000
42 100
36 300
24 ×1.8 mL
24 ×1.8 mL
J-TB-037
JA-18 Fixed Angle, 23° (10 place)
rmax = 132 mm 18 000 47 900 10 ×100 mL J-TB-035
JA-17 Fixed Angle, 25° (6 place)
rmax = 132 mm 17 000 39 800 14 ×50 mL J-TB-017
JLA-16.250 Fixed Angle, 25° (6 place)
rmax = 134 mm 16 000 38 400 6 ×250 mL J-TB-072
JA-14 Fixed Angle, 25° (6 place)
rmax = 137 mm 14 000 30 100 6 ×250 mL J-TB-004
—Continued

1-5
Specifications and Preinstallation Requirements
aMaximum speeds are based on a solution density of 1.2 g/mL with the following exceptions: the JA-18.1 rotor is rated for a density of 1.4 g/mL;
the JCF-Z rotor is rated for a density of 1.45 g/mL; and the JE-6B rotor is rated for a density of 3 g/mL.
bMaximum speed for the JA-30.50 Ti rotor is 25 000 rpm in the Avanti J-2 I centrifuge, and 30 000 rpm in the Avanti J-30I centrifuge.
cMaximum speed for the JS-24.38 and JS-24.15 rotors is 10 000 rpm in the Avanti J-25I centrifuge, and 24 000 rpm in the Avanti J-30I centrifuge.
Rotor Profile and Description Max
RPMa
Max
RCF
(×g)Max Capacity
Rotor
Manual
Number
JA-12 Fixed Angle, 35° (12 place) rmax = 144 mm 12 000 23 200 12 ×50 mL J-TB-051
JA-10 Fixed Angle, 25° (6 place)
rmax = 158 mm 10 000 17 700 6 ×500 mL J-TB-006
JLA-10.500 Fixed Angle, 20° (6 place)
rmax = 166 mm 10 000 18 500 6 ×500 mL J-TB-048
F10BCI-6x500y Fixed Angle, 23° (6 place)
rmax = 158 mm 10 000 17 696 6 ×500 mL —
JLA-9.1000 Fixed Angle, 20° (4 place)
rmax = 185 mm 9 000 16 800 4 ×1000 mL J-TB-073
JS-24.38 Swinging Bucket (6 place)
rmax = 161.0 mm 10 000c18 000 6 ×38.5 mL J-TB-058
—Continued

1-6
Specifications and Preinstallation Requirements
aMaximum speeds are based on a solution density of 1.2 g/mL with the following exceptions: the JA-18.1 rotor is rated for a density of 1.4 g/mL; the
JCF-Z rotor is rated for a density of 1.45 g/mL; and the JE-6B rotor is rated for a density of 3 g/mL.
bMaximum speed for the JA-30.50 Ti rotor is 25 000 rpm in the Avanti J-25I centrifuge, and 30 000 rpm in the Avanti J-30I centrifuge.
cMaximum speed for the JS-24.38 and JS-24.15 rotors is 10 000 rpm in the Avanti J-25I centrifuge, and 24 000 rpm in the Avanti J-30I centrifuge.
Rotor Profile and Description Max RPMa
Max
RCF
(×g)Max Capacity
Rotor
Manual
Number
JS-24.15 Swinging Bucket (6 place)
rmax = 171.3 mm 10 000c19 200 6 ×15 mL J-TB-058
JS-13.1 Swinging Bucket (6 place)
rmax = 140 mm 13 000 26 500 6 ×50 mL J-TB-036
JS-7.5 Swinging Bucket (4 place)
rmax = 165 mm 7 500 10 400 4 ×50 mL J-TB-007
JS-5.9 Swinging Bucket (2 place)
rmax = 168.5 mm 5 900 6 570 10 microplates
4 deep-well plates
2 square-well plates
J-TB-092
JCF-Z Continuous Flow/Zonal Rotor
20 000 — 660 mL
(standard core)
1250 mL
(large core)
240 mL
(small core)
JCFZ-IM
JE-6B Elutriation Rotor
rmax
standard
chamber = 125 mm
Sanderson
chamber = 126 mm
6 000
5 040
5 080
4.2 mL
(standard chamber)
4.8 mL
(Sanderson chamber)
JE6B-IM

1-7
Specifications and Preinstallation Requirements
PREINSTALLATION REQUIREMENTS
Do not attempt to install this instrument. Its purchase price includes
installation by Beckman Coulter personnel. Installation by anyone
other than an authorized Beckman Coulter representative invalidates
any warranty covering the instrument.
Preinstallation requirements have been sent prior to shipment of the
instrument. The following information is provided in case the centri-
fuge must be relocated.
The centrifuge will be installed by a Beckman Coulter Field Service
representative after preinstallation requirements for power and site
preparation have been met. The following equipment is required for
preinstallation:
• Voltmeter
• Single phase: two 30-ampere circuit breakers
• Three phase: three 16-ampere circuit breakers
• Power receptacle (see Figure 1-1 or 1-2)
• Drill for drilling holes in the floor for installation of anchoring kit
bolts (see SECURING THE CENTRIFUGE TO THE FLOOR, later in
this section). A 9.5 mm (3/8-inch) drill is required for concrete
floors. A 6.4 mm (1/4-inch) drill is required for wood floors.
ELECTRICAL REQUIREMENTS
Power to the centrifuge should originate directly from a main power
line transformer at a power source known to be clear of erratic loads,
spikes, and electromagnetic interference. Make sure that there are
properly rated thermal circuit breakers at the service panel to protect
the centrifuge circuit. If fuses must be used instead of the specified
circuit breakers, the fuses may require a rating of greater than
30 Amperes (for single-phase centrifuges) or greater than
16 Amperes (for three-phase centrifuges).
Terminate the open end of the harmonized cord with a certified
single- or three-phase connector suitable for the power supplied in the
country of intended use (see Table 1-1). Install only one centrifuge
per circuit.
To ensure safety, connect the instrument to a remote emergency
switch (preferably outside the room where the centrifuge is housed,
or adjacent to the exit from that room), in order to disconnect the
centrifuge from the main power source in case of malfunction.