Binder APT.line KB Series User manual

Issue 08/2012 Art. No. 7001-0115
Operating Manual
APT.line™ KB (E3.1)
Refrigerated Incubators
with microprocessor program controller RD3
Model
Art. No.
KB 23 (E3.1)
9020-0112, 9120-0112
KB 23-UL (E3.1)
9020-0113, 9120-0113
KB 53 (E3.1)
9020-0114, 9120-0114
9020-0243, 9120-0243
KB 53-UL (E3.1)
9020-0115, 9120-0115
KB 53 (115 V) (E3.1)
9020-0252, 9120-0252
KB 115 (E3.1)
9020-0116, 9120-0116
9020-0242, 9120-0242
KB 115-UL (E3.1)
9020-0117, 9120-0117
KB 115 (115 V) (E3.1)
9020-0253, 9120-0253
BINDER GmbH
Address Post office box 102
D-78502 Tuttlingen
Tel. [phone]
Fax [phone]
Internet http://www.binder-world.com
E-mail [e-mail]
Service Hotline [phone]
Service Fax [phone]
Service E-Mail [e-mail]
Service Hotline USA [phone] or [phone] x3
Service Hotline Asia Pacific [phone] or [phone]
Service Hotline Russia and CIS [phone]

KB (E3.1) 08/2012 page 2/84
EC – declaration of conformity
EG - KONFORMITÄTSERKLÄRUNG
EC - DECLARATION OF CONFORMITY
CE - DECLARATION DE CONFORMITE
Anbieter / Supplier / Fournisseur: BINDER GmbH
Anschrift / Address / Adresse: Im Mittleren Ösch 5, D-78532 Tuttlingen
Produkt / Product / Produit: Kühlbrutschränke mit Programmregelung
Refrigerated incubators with program control
Incubateurs réfrigérés à régulation programmable
Typenbezeichnung / Type / Type: KB 23, KB 53, KB 115
Die oben beschriebenen Produkte sind konform mit folgenden EG-Richtlinien:
The products described above are in conformity with the following EC guidelines:
Les produits décrits ci-dessus sont conformes aux directives CE suivantes:
Niederspannungsrichtlinie
2006/95/EG
Low voltage directive
2006/95/EC
Directive basse tension
2006/95/CE
Richtlinie 2006/95/EG des Europäischen Parlaments und des Rates
vom 12. Dezember 2006 zur Angleichung der Rechtsvorschriften der
Mitgliedstaaten betreffend elektrische Betriebsmittel zur Verwendung
innerhalb bestimmter Spannungsgrenzen
Council Directive 2006/95/EC of 12 December 2006 on the
harmonization of the laws of Member States relating to electrical
equipment designed for use within certain voltage limits
Directive 2006/95/CE du Parlement Européen et du Conseil du 12
décembre 2006 concernant le rapprochement des législations des États
membres relatives au matériel électrique destiné à être employé dans
certaines limites de tension
EMV-Richtlinie
2004/108/EG
EMC Directive
2004/108/EC
Directive CEM
2004/108/CE
Richtlinie 2004/108/EG des Europäischen Parlaments und des Rates
vom 15. Dezember 2004 zur Angleichung der Rechtsvorschriften der
Mitgliedstaaten über die elektromagnetische Verträglichkeit und zur
Aufhebung der Richtlinie 89/336/EWG.
Directive 2004/108/EC of the European Parliament and of the Council
of 15 December 2004 on the approximation of the laws of the Member
States relating to electromagnetic compatibility and repealing Directive
98/336/EEC.
Directive 2004/108/CE du Parlement Européen et du Conseil du 15
décembre 2004 relative au rapprochement des législations des États
membres concernant la compatibilité électromagnétique et abrogeant le
directive 98/336/CEE.
Die oben beschriebenen Produkte tragen entsprechend die Kennzeichnung CE.
The products described above, corresponding to this, bear the CE-mark
Les produits décrits ci-dessus, en correspondance, portent l’indication CE.
1 / 3

KB (E3.1) 08/2012 page 3/84
Die oben beschriebenen Produkte sind konform mit folgenden harmonisierten Normen:
The products described above are in conformity with the following harmonized standards:
Les produits décrits ci-dessus sont conformes aux normes harmonisées suivantes:
Sicherheit / safety / sécurité:
EN 61010-1:2010 Sicherheitsbestimmungen für elektrische Mess-, Steuer-, Regel- und
Laborgeräte – Teil 1: Allgemeine Anforderungen (DIN EN 61010-
1:2011, VDE 411-1:2011)
Safety requirements for electrical equipment for measurement, control,
and laboratory use – Part 1: General requirements (IEC 61010-1:2010,
BS EN 61010-1:2010)
Règles de sécurité pour appareils électriques de mesurage, de
régulation et de laboratoire – Partie 1: Prescriptions générales (CEI
61010-1:2010, NF EN 61010:2011)
EN 61010-2-010:2003 Sicherheitsbestimmungen für elektrische Meß-, Steuer-, Regel- und
Laborgeräte – Teil 2-010: Besondere Anforderungen an Laborgeräte für
das Erhitzen von Stoffen (DIN EN 61010-2-010:2004)
Safety requirements for electrical equipment for measurement, control,
and laboratory use – Part 2-010: Particular requirements for laboratory
equipment for the heating of materials (IEC 61010-2-10:2005, BS EN
61010-2-10:2003)
Règles de sécurité pour appareils électriques de mesurage, de
régulation et de laboratoire – Partie 2-010 : Prescriptions particulières
pour appareils de laboratoire utilisés pour l’échauffement des matières
(CEI 61010-2-10:2003, NF EN 61010-2-10:2005)
EMV / EMC / CEM:
EN 61326-1:2006
+ Corr. 1:2008 + Corr. 2:2010
Elektrische Mess-, Steuer-, Regel- und Laborgeräte - EMV-
Anforderungen - Teil 1: Allgemeine Anforderungen (DIN EN 61326-
1:2006 + Berichtigung 1:2008 + Berichtigung 2:2011)
Electrical equipment for measurement, control and laboratory use -
EMC requirements - Part 1: General requirements (IEC 61326-1:2005 +
Corr. 1:2008 + Corr. 2:2010, BS EN 61326-1:2006+ A1:2008)
Matériel électrique de mesure, de commande et de laboratoire -
Exigences relatives à la CEM - Partie 1: Exigences générales (CEI
61326-1:2005 + AC1:2008, NF EN 61326-1:2006 mod.)
EN 61326-2-2:2006 Elektrische Mess-, Steuer-, Regel- und Laborgeräte – EMV-
Anforderungen. Teil 2-2: Besondere Anforderungen - Prüfanordnung,
Betriebsbedingungen und Leistungsmerkmale für ortsveränderliche
Prüf-, Mess- und Überwachungsgeräte in Niederspannungs-
Stromversorgungsnetzen. (DIN EN 61326-2-2:2006)
Electrical equipment for measurement, control and laboratory use –
EMC requirements. Part 2-2: Particular requirements - Test
configurations, operational conditions and performance criteria for
portable test, measuring and monitoring equipment used in low-voltage
distribution systems. (IEC 61326-2-2:2005, BS EN 61326-2-2:2006)
Matériel électrique de mesure, de commande et de laboratoire –
Exigences relatives à la CEM. Partie 2-2: Exigences particulières -
Configurations d’essai, conditions de fonctionnement et critères
d’aptitude à la fonction des matériels portatifs d’essai, de mesure et de
surveillance utilisés dans des systèmes de distribution basse tension.
(CEI 61326-2-2:2005 + AC1:2007, NF EN 61326-2-2:2006)
2 / 3

KB (E3.1) 08/2012 page 4/84
D-78532 Tuttlingen, 16.01.2012
BINDER GmbH
P. M. Binder
Geschäftsführender Gesellschafter
Managing Director
Directeur général
Dr. H. von Both
Leiter F & E
Director R & D
Chef de service R&D
3 / 3

KB (E3.1) 08/2012 page 5/84
Product registration

KB (E3.1) 08/2012 page 6/84
Contents
EC – declaration of conformity ......................................................................................................................2
Product registration .......................................................................................................................................5
1. SAFETY.................................................................................................................. 8
1.1 Legal considerations ...........................................................................................................................8
1.2 Structure of the safety instructions......................................................................................................8
1.2.1 Signal word panel .....................................................................................................................8
1.2.2 Safety alert symbol ...................................................................................................................9
1.2.3 Pictograms................................................................................................................................9
1.2.4 Word message panel structure...............................................................................................10
1.3 Localization / position of safety labels on the unit.............................................................................10
1.4 Type plate .........................................................................................................................................11
1.5 General safety instructions on installing and operating the refrigerated incubator ..........................12
1.6 Intended use .....................................................................................................................................13
2. UNIT DESCRIPTION ............................................................................................ 14
2.1 Unit overview.....................................................................................................................................15
2.2 Instrument panel ...............................................................................................................................15
3. COMPLETENESS OF DELIVERY, TRANSPORTATION, STORAGE, AND
INSTALLATION.................................................................................................... 16
3.1 Unpacking, and checking equipment and completeness of delivery................................................16
3.2 Guidelines for safe lifting and transportation ....................................................................................16
3.3 Storage..............................................................................................................................................17
3.4 Location of installation and ambient conditions ................................................................................17
4. INSTALLATION OF THE EQUIPMENT ............................................................... 18
4.1 Electrical connection .........................................................................................................................18
5. START UP............................................................................................................ 19
5.1 Settings at the RD3 program controller ............................................................................................19
5.2 General indications ...........................................................................................................................20
6. FIXED VALUE ENTRY MODE ............................................................................. 21
7. WEEK PROGRAM EDITOR................................................................................. 23
7.1 Program table template for Week program Editor ............................................................................25
8. PROGRAM EDITOR............................................................................................. 26
8.1 Selecting between set-point ramp and set-point step.......................................................................26
8.1.1 Programming with setting “Ramp” (default setting) ................................................................26
8.1.2 Programming with setting “step”.............................................................................................28
8.1.3 General notes on programming temperature transitions........................................................29
8.2 Set-point entry for program operation...............................................................................................29
8.3 Program table template for Program Editor......................................................................................33
8.4 Deleting a program section...............................................................................................................34
9. PROGRAM START LEVEL.................................................................................. 35
10. USER LEVEL ....................................................................................................... 37
11. PROGRAMMING EXAMPLE OF THE WEEK PROGRAM EDITOR.................... 45
11.1 Desired time function ........................................................................................................................45
11.2 Proceeding overview.........................................................................................................................45
11.3 Proceeding in detail...........................................................................................................................45

KB (E3.1) 08/2012 page 7/84
12. EXAMPLE PROGRAMMING FOR THE PROGRAM EDITOR............................. 52
12.1 Desired time function ........................................................................................................................52
12.2 Proceeding overview.........................................................................................................................52
12.3 Proceeding in detail...........................................................................................................................53
13. BEHAVIOR IN CASE OF FAILURES................................................................... 59
13.1 Behavior after power failure ..............................................................................................................59
13.2 Alarm messages ...............................................................................................................................59
14. TEMPERATURE SAFETY DEVICES................................................................... 59
14.1 Over temperature protective device (class 1)...................................................................................59
14.2 Safety controller (temperature safety device class 3.1)....................................................................59
14.3 Temperature safety device class 3.3 (option from size 53 on).........................................................59
15. NOTES ON REFRIGERATING OPERATION ...................................................... 62
16. OPTIONS.............................................................................................................. 62
16.1 Communication software APT-COM™ 3 DataControlSystem (option) ............................................62
16.2 Ethernet interface (option) ................................................................................................................62
16.3 Protocol printer (option from size 53 on)...........................................................................................63
16.4 Data logger kit...................................................................................................................................63
16.5 Additional Pt100 temperature sensor (option from size 53 on) ........................................................64
16.6 Analog output for temperature (option from size 53 on) ...................................................................64
16.7 Zero-voltage relay outputs via operation lines (option from size 53 on)...........................................64
16.8 Water protected internal socket (option from size 53 on) .................................................................65
17. MAINTENANCE, CLEANING, AND SERVICE .................................................... 66
17.1 Maintenance intervals, service..........................................................................................................66
17.2 Cleaning and decontamination .........................................................................................................67
17.2.1 Cleaning..................................................................................................................................67
17.2.2 Decontamination.....................................................................................................................68
17.3 Sending the unit back to BINDER GmbH .........................................................................................69
18. DISPOSAL............................................................................................................ 70
18.1 Disposal of the transport packing .....................................................................................................70
18.2 Decommissioning..............................................................................................................................70
18.3 Disposal of the unit in the Federal Republic of Germany .................................................................70
18.4 Disposal of the unit in the member states of the EC except for the Federal Republic of Germany .72
18.5 Disposal of the unit in non-member states of the EC .......................................................................73
19. TROUBLESHOOTING ......................................................................................... 73
20. TECHNICAL DESCRIPTION................................................................................ 75
20.1 Factory calibration and adjustment...................................................................................................75
20.2 Over current protection .....................................................................................................................75
20.3 Definition of usable volume...............................................................................................................75
20.4 KB technical data ..............................................................................................................................76
20.5 Equipment and Options ....................................................................................................................77
20.6 Spare parts and accessories ............................................................................................................78
21. CONTAMINATION CLEARANCE CERTIFICATE ............................................... 79
21.1 For units located outside North America and Central America.........................................................79
21.2 For units in North America and Central America ..............................................................................82

KB (E3.1) 08/2012 page 8/84
Dear customer,
For the correct operation of the refrigerated incubator KB, it is important that you read this operating
manual completely and carefully and observe all instructions as indicated. Failure to read, understand
and follow the instructions may result in personal injury. It can also lead to damage to the unit and/or poor
equipment performance.
1. Safety
This operating manual is part of the components of delivery. Always keep it handy for reference. The
device should only be operated by laboratory personnel especially trained for this purpose and familiar
with all precautionary measures required for working in a laboratory. To avoid injuries and damage
observe the safety instructions of the operating manual.
WARNING
Failure to observe the safety instructions.
Serious injuries and unit damage.
Observe the safety instructions in this operating manual.
Carefully read the complete operating instructions of the refrigerated incubator KB.
1.1 Legal considerations
This operating manual is for informational purposes only. It contains information for installing, start-up,
operation and maintenance of the product. Note: the contents and the product described are subject to
change without notice.
Understanding and observing the instructions in this operating manual are prerequisites for hazard-free
use and safety during operation and maintenance. In no event shall BINDER be held liable for any
damages, direct or incidental arising out of or related to the use of this manual.
This operating manual cannot cover all conceivable applications. If you would like additional information,
or if special problems arise that are not sufficiently addressed in this manual, please ask your dealer or
contact us directly by phone at the number located on page one of this manual
Furthermore, we emphasize that the contents of this operating manual are not part of an earlier or
existing agreement, description, or legal relationship, nor do they modify such a relationship. All
obligations on the part of BINDER derive from the respective purchase contract, which also contains the
entire and exclusively valid statement of warranty administration. The statements in this manual neither
augment nor restrict the contractual warranty provisions.
1.2 Structure of the safety instructions
In this operating manual, the following safety definitions and symbols indicate dangerous situations
following the harmonization of ISO 3864-2 and ANSI Z535.6.
1.2.1 Signal word panel
Depending on the probability of serious consequences, potential dangers are identified with a signal
word, the corresponding safety color, and if appropriate, the safety alert symbol.
DANGER
Indicates an imminently hazardous situation that, if not avoided, will result in death or serious
(irreversible) injury.
WARNING
Indicates a potentially hazardous situation which, if not avoided, could result in death or serious
(irreversible) injury.

KB (E3.1) 08/2012 page 9/84
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor
(reversible) injury.
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in damage to the product
and/or its functions or of a property in its proximity.
1.2.2 Safety alert symbol
Use of the safety alert symbol indicates a risk of injury.
Observe all measures that are marked with the safety alert symbol in order to avoid death or
injury.
1.2.3 Pictograms
Warning signs
Electrical hazard
Hot surface
Explosive atmosphere
Stability hazard
Lifting hazard
Risk of corrosion and /
or chemical burns
Harmful substances
Biohazard
Pollution Hazard
Mandatory action signs
Mandatory regulation Read operating
instructions
Disconnect the power
plug
Lift with several persons
Environment protection
Wear protective gloves
Wear safety goggles
Prohibition signs
Do NOT touch
Do NOT spray with
water
Do NOT climb

KB (E3.1) 08/2012 page 10/84
Information to be observed in order to ensure optimum function of the product.
1.2.4 Word message panel structure
Type / cause of hazard.
Possible consequences.
∅Instruction how to avoid the hazard: prohibition
Instruction how to avoid the hazard: mandatory action
Observe all other notes and information not necessarily emphasized in the same way, in order to avoid
disruptions that could result in direct or indirect injury or property damage.
1.3 Localization / position of safety labels on the unit
The following labels are located on the unit:
Pictograms (Warning signs)
Service label
Hot surface
Risk of injury (UL units only)
Unit front KB
Unit front KB-UL
Figure 1: Position of labels on the unit
Keep safety labels complete and legible.
Replace safety labels that are no longer legible. Contact BINDER service for these replacements.

KB (E3.1) 08/2012 page 11/84
1.4 Type plate
Figure 2: Position of type plate
Figure 3: Type plate (example of KB 115 regular unit 9020-0122)
Indications of the type plate
Information
BINDER
Manufacturer: BINDER GmbH
KB 115
Model KB 115
Serial No.
00-00000
Serial No. 00-00000
Nominal temperature 100 °C
212 °F
Nominal temperature
Enclosure protection
IP 20
IP type of protection 20 acc. to EN 60529
Temp. safety device
DIN 12880
Temperature safety device acc. to standard DIN 12880
Class
3.1
Temperature safety device, class 3.1
Art. No.
9020-0122
Art. No. 9020-0122
Project No.
---
(Special application acc. to project no.)
0,46 kW
Nominal power 0.46 kW
230 V 1 N ~
Nominal voltage 230 V
±
10%, single-phase unit
2,0 A
Nominal current 2.0 Amp
50/60 Hz
Power frequency 50/60 Hz
Max. operating pressure 15 bar
Max. operating pressure 15 bar in the refrigerating system
R 134 A - 0,145 kg
Refrigerant type R 134 A, max. filling weight 0.145 kg
Contains fluorinated greenhouse gases
covered by the Kyoto Protocol
Contains fluorinated greenhouse gases covered by the
Kyoto Protocol
Nominal temperature
100 °C
0,46 kW
Max. operating pressure 15 bar
212°F
230 V 1 N ~
R 134 A – 0,145 kg
Enclosure protection
Temp. safety device
IP 20
DIN 12880
2,0 A
50/60 Hz
Contains fluorinated greenhouse
gases
Class
3.1
covered by the Kyoto Protocol
Art. No.
9020-0116
US PATS 4585923 / 5222612 / 5309981
Project No.
5405194 / 5601143 / 5773287 / 6079403
D 78532 Tuttlingen / Germany
Tel. + 49 (0) 7462/ 2005-0
Internet: www.binder-world.com
KB 115 Serial No. 00-00000
Made in Germany

KB (E3.1) 08/2012 page 12/84
Symbol on the type plate
Information
CE conformity marking
Electrical and electronic equipment manufactured / placed
on the market in the EC after 13 August 2005 and to be
disposed of in separate collection according to directive
2002/96/EC on waste electrical and electronic equipment
(WEEE).
The equipment is certified in the GOST R certification
system of GOSTSTANDARD Russia.
LABORATORY EQUIPMENT
43KM
(KB-UL only)
The equipment is certified by Underwriters Laboratories
Inc.®according to standards UL 61010A-1, UL 61010A-2-
10, CSA C22.2 No. 1010.1-92, and CSA C22.2 No.
1010.2.010-94.
1.5 General safety instructions on installing and operating the refrigerated
incubator
With regard to operating the refrigerated incubator KB and to the installation location, please observe the
guideline BGI/GUV-I 850-0 on safe working in laboratories (formerly BGR/GUV-R 120 or ZH 1/119
laboratory guidelines issued by the employers’ liability insurance association) (for Germany).
BINDER GmbH is only responsible for the safety features of the unit provided skilled electricians or
qualified personnel authorized by BINDER perform all maintenance and repair, and if components
relating to chamber safety are replaced in the event of failure with original spare parts.
To operate the unit, use only original BINDER accessories or accessories from third-party suppliers
authorized by BINDER. The user is responsible for any risk caused by using unauthorized accessories.
CAUTION
Danger of overheating.
Damage to the unit.
∅Do NOT install the unit in unventilated recesses.
Ensure sufficient ventilation for dispersal of the heat.
Do not operate the refrigerated incubator KB in hazardous locations.
DANGER
Explosion hazard.
Danger of death.
∅Do NOT operate the unit in potentially explosive areas.
KEEP explosive dust or air-solvent mixtures AWAY from the unit.

KB (E3.1) 08/2012 page 13/84
The refrigerated incubator KB does not dispose of any measures of explosion protection.
DANGER
Explosion hazard.
Danger of death.
∅Do NOT introduce any substance into the refrigerated incubator which is combustible
or explosive at working temperature.
∅NO explosive dust or air-solvent mixture in the inner chamber.
Any solvent contained in the charging material must not be explosive or inflammable. I.e., irrespective of
the solvent concentration in the steam room, NO explosive mixture with air must form. The temperature
inside the chamber must lie below the flash point or below the sublimation point of the charging material.
Familiarize yourself with the physical and chemical properties of the charging material, as well as the
contained moisture constituent and its behavior with the addition of heat energy.
Familiarize yourself with any potential health risks caused by the charging material, the contained
moisture constituent or by reaction products which may arise during the temperature process. Take
adequate measures to exclude such risks prior to putting the refrigerated incubator into operation.
DANGER
Electrical hazard.
Danger of death.
∅The unit must NOT become wet during operation or maintenance.
The refrigerated incubators were produced in accordance with VDE regulations and were routinely tested
in accordance to VDE 0411-1 (IEC 61010-1).
CAUTION
The glass doors and the inner chamber will become hot during operation.
Danger of burning.
∅Do NOT touch the glass doors, the inner surfaces or the charging material during
operation.
WARNING
Stability hazard.
Danger of injury.
Damage to the unit and the charging material.
Housing cover breakaway.
∅Do NOT climb on the lower housing cover.
∅Do NOT load the lower housing cover with heavy objects while the unit door is open.
1.6 Intended use
Refrigerated incubators KB are suitable for exact conditioning of harmless materials. Because of their
precise temperature accuracy these devices are especially useful for cultivation of microorganisms with a
narrow temperature optimum in a range of 5 °C / 41 °F to 40 °C / 104 °F. Main fields of application are
tests of long-term storage (e.g. at 5 °C), refrigerated incubation between 20 °C / 68 °F and 30 °C / 86 °F
and incubation with alternating temperatures (e.g. 40/5 °C / 104/41 °F ).

KB (E3.1) 08/2012 page 14/84
A mixture of any component of the charging material with air must NOT be explosive. The operating
temperature must lie below the flash point or below the sublimation point of the charging material.
Following the instructions in this operating manual and conducting regular maintenance work
(chap. 17.1) are part of the intended use.
Other applications are not approved.
The charging material shall not contain any corrosive ingredients that may damage the
machine components made of stainless steel, aluminum, and copper. Such ingredients
include in particular acids and halides. Any corrosive damage caused by such ingredients is
excluded from liability by BINDER GmbH.
WARNING: If customer should use a BINDER chamber running in non-supervised
continuous operation, we strongly recommend in case of inclusion of irrecoverable specimen
or samples to split such specimen or samples and store them in at least two chambers, if this
is feasible.
2. Unit description
A high level of precision, reliability, and safety for all growth parameters ensures optimum incubation
conditions. Moreover, the KB refrigerated incubator is designed for maximum usability – even in
continuous operation year after year. It fulfills all technical and application-specific requirements arising in
experimentation such as in the areas of biotechnology, medicine, the nutrition industry, pharmaceutical
and cosmetics industries, botany, and zoology.
Two important temperature technologies have been combined to achieve perfect temperature control.
The newly developed DCT™ refrigerating system, a direct refrigerating process, in conjunction with the
APT.line™ preheating chamber technology, satisfies the unique prerequisites for attaining highly-precise
temperature control and particularly short recovery times after opening the door.
The refrigerating system is distinguished by direct, precise, and rapid temperature conduction. Large-
area labyrinth evaporator plates are integrated into the outer walls of the preheating chamber system. In
contrast to the indirect systems, such as the air-jacket system, they conduct the cold directly to the
atmosphere of the working space.
The APT.line™ preheating chamber system guarantees high level of spatial and time-based temperature
precision, thanks to the direct and distributed air circulation into the interior. This is especially important
for maintaining temperatures – especially with full chambers – and for rapid restoration of optimum
growth conditions after opening the door. The inner glass door ensures that the temperature remains
constant when observing the incubation process. The air turbine supports exact attainment and
maintenance of the desired temperature accuracy. The fan speed is digitally adjustable from 0 % to
100%. The heating and refrigerating systems are microprocessor regulated to a tenth of a degree. In
addition, the refrigerated incubator provides almost unlimited possibilities for adaptation to individual
customer requirements based upon extensive programming options and on the week program timer and
real time clock of the controller.
All unit functions are easy and comfortable to use thanks to their clear arrangement. Major features are
easy cleaning of all unit parts and avoidance of undesired contamination.
The inner chamber, the pre-heating chamber and the interior side of the doors are all made of stainless
steel (material no. 1.4301 (V2A) in Germany, US equivalent 304). The housing is RAL 7035 powder-
coated. All corners and edges are also completely coated.
The refrigerated incubator KB is equipped with a serial interface RS 422 for computer communication,
e.g. via the communication software APT-COM™ 3 DataControlSystem (option, chap. 16.1). For further
options, see chap. 20.5.
Temperature range at ambient temperature of max. 25 °C / 77 °F: -5 °C / 23 °F up to +100 °C / 212 °F.

KB (E3.1) 08/2012 page 15/84
2.1 Unit overview
Figure 4: Refrigerated incubator KB
(1) Main power switch On/Off
(2) Temperature controller RD3
(3) Instrument panel
(4) Unit door
(5) Compressor housing
2.2 Instrument panel
(2) (6) (1)
Figure 5: Control panel of standard unit
(1) Main power switch On/Off
(2) Program controller RD3
(6) Switch for interior illumination (option)
(2)
(1)
(4)
(3)
(5)
B NEI

KB (E3.1) 08/2012 page 16/84
3. Completeness of delivery, transportation, storage, and
installation
3.1 Unpacking, and checking equipment and completeness of delivery
After unpacking, please check the unit and its optional accessories, if any, based on the delivery note for
completeness and for transportation damage. If transportation damage has occurred, inform the carrier
immediately.
The final tests of the manufacturer may cause traces of the racks on the inner surfaces. This has no
impact on the function and performance of the unit.
Please remove any transportation protection devices and adhesives in/on the unit and on the doors and
take out the operating manuals and accessory equipment.
CAUTION
Sliding or tilting of the unit.
Damage to the unit.
Risk of injury by lifting heavy loads.
∅Do NOT lift or transport the unit using the door, the handle, or the lower housing.
Lift the unit from the pallet at the four lower corners with the aid of four people.
If you need to return the unit, please use the original packing and observe the guidelines for safe lifting
and transportation (chap. 3.2).
For disposal of the transport packing, see chap. 18.1.
Note on second-hand units (Ex-Demo-Units):
Second-hand units are units that were used for a short time for tests or exhibitions. They are thoroughly
tested before resale. BINDER ensures that the chamber is technically sound and will work flawlessly.
Second-hand units are marked with a sticker on the unit door. Please remove the sticker before
commissioning the unit.
3.2 Guidelines for safe lifting and transportation
After operation, please observe the guidelines for temporary decommissioning (chap. 18.2).
CAUTION
Sliding or tilting of the unit.
Damage to the unit.
Risk of injury by lifting heavy loads.
Transport the unit in its original packaging only.
For moving or shipping, secure the unit with transport straps.
∅Do NOT lift or transport the unit using the door, the handle, or the lower housing.
Lift the unit at the four lower corners with the aid of 4 people.
•Permissible ambient temperature range during transport: -10 °C to +60 °C.
You can order transport packing and rolling pallets for moving or shipping purposes from BINDER
service.

KB (E3.1) 08/2012 page 17/84
3.3 Storage
Intermediate storage of the unit is possible in a closed and dry room. Observe the guidelines for
temporary decommissioning (chap. 18.2).
•Permissible ambient temperature range during storage: -10 °C to +60 °C.
•Permissible ambient humidity: max. 70 % r.H., non-condensing
When after storage in a cold location you transfer the unit to its warmer installation site, condensation
may form. Before start-up, wait at least one hour until the chamber has attained ambient temperature and
is completely dry.
3.4 Location of installation and ambient conditions
Set up the KB refrigerated incubator on a flat, even surface, free from vibration and in a well-ventilated,
dry location and align it using a spirit level. The site of installation must be capable of supporting the unit’s
weight (see technical data, chap. 20.4). The chambers are designed for setting up inside a building
(indoor use).
CAUTION
Danger of overheating.
Damage to the unit.
∅Do NOT set up units in non-ventilated recesses.
Ensure sufficient ventilation for dispersal of the heat.
•Permissible ambient temperature range during operation: +18 °C to +32 °C. At elevated ambient
temperature values, fluctuations in temperature can occur.
The ambient temperature should not be substantially higher than the indicated ambient
temperature of +25 °C to which the specified technical data relate. For other ambient
conditions, deviations from the indicated data are possible.
With each degree of ambient temperature >25 °C, the refrigeration power decreases by 1.5 K.
•Permissible ambient humidity: 70 % r.H. max., non-condensing.
When operating the chamber at temperature set-points below ambient temperature, high ambient
humidity may lead to condensation on the unit.
•Installation height: max. 2000 m / 6.6 ft. above sea level.
When placing several units of the same size side by side, maintain a minimum distance of 250 mm
between each unit. Wall distances: rear 100 mm / 3.94 in, sides 160 mm / 6.29 in. Spacing above the unit
of at least 100 mm / 3.94 in must also be maintained.
Two devices up to size 115l can be piled on top of each other. For this purpose place rubber pads under
every foot of the upper unit to prevent the device from slipping.
CAUTION
Sliding or tilting of the upper unit.
Damage to the units.
When stacking, place rubber pads under every foot of the upper unit.
To completely separate the unit from the power supply, you must disconnect the power plug. Install the
unit in a way that the power plug is easily accessible and can be easily pulled in case of danger.
With an increased amount of dust in the ambient air, clean the condenser fan (by suction or blowing)
several times a year.

KB (E3.1) 08/2012 page 18/84
Avoid any conductive dust in the ambiance according to the unit layout complying with pollution degree 2
(IEC 61010-1).
Do not install or operate the unit in potentially explosive areas.
DANGER
Explosion hazard.
Danger of death.
∅Do NOT operate the unit in potentially explosive areas.
∅KEEP explosive dust or air-solvent mixtures AWAY from the vicinity of the unit.
4. Installation of the equipment
4.1 Electrical connection
•The refrigerated incubator comes with a fixed power connection cable that has a length of 1800 mm /
5.9 ft and is equipped with a semi time-lag fine-wire fuse.
Model Art. no.
(x = 0 or 1) Power plug Voltage
+ /-10 %
Current
type
Power
frequency Unit fuse
KB 23 9x20-0112 Shock-proof plug 230 V 1N~ 50/60 Hz 10 A
KB 53 9x20-0114 Shock-proof plug 230 V 1N~ 50/60 Hz 10 A
KB 53 9x20-0243 Shock-proof plug 230 V 1N~ 50 Hz 10 A
KB 115 9x20-0116 Shock-proof plug 230 V 1N~ 50/60 Hz 10 A
KB 115 9x20-0242 Shock-proof plug 230 V 1N~ 50 Hz 10 A
KB 23-UL 9x20-0113 NEMA 5-15 115 V 1N~ 60 Hz 12.5 A
KB 53-UL 9x20-0115 NEMA 5-15 115 V 1N~ 60 Hz 12.5 A
KB 53 (115V)
9x20-0252 NEMA 5-15 115 V 1N~ 60 Hz 12.5 A
KB 115-UL 9x20-0117 NEMA 5-15 115 V 1N~ 60 Hz 12.5 A
KB 115 (115V)
9x20-0253 NEMA 5-15 115 V 1N~ 60 Hz 12.5 A
•Prior to connection and start-up, check the power supply voltage. Compare the values to the specified
data located on the unit’s type plate (unit front behind the door, bottom left-hand, see chap. 1.4).
•When connecting, please observe the regulations specified by the local electricity supply company as
well as the VDE directives (for Germany)
•Pollution degree (acc. to IEC 61010-1): 2
•Over-voltage category (acc. to IEC 61010-1): II
CAUTION
Danger of incorrect power supply voltage.
Damage to the equipment.
Check the power supply voltage before connection and start-up.
Compare the power supply voltage with the data indicated on the type plate.
See also electrical data (chap. 20.4).

KB (E3.1) 08/2012 page 19/84
To completely separate the unit from the power supply, you must disconnect the power plug.
Install the unit in a way that the power plug is easily accessible and can be easily pulled in
case of danger.
5. Start up
After connecting the electrical supply (chap. 4.1), turn on the unit via the main power switch (1).
Warming chambers may release odors in the first few days after commissioning. This is not a quality
defect. To reduce odors quickly we recommend heating up the chamber to its nominal temperature for
one day and in a well-ventilated location.
WARNING: If customer should use a BINDER chamber running in non-supervised
continuous operation, we strongly recommend in case of inclusion of irrecoverable specimen
or samples to split such specimen or samples and store them in at least two chambers, if this
is feasible.
5.1 Settings at the RD3 program controller
After turning the unit on with the main power switch (1) the controller is in Normal Display / fixed value
operation mode.
Depending on the temperature value entered before LED (3a) is lit if the heating is active, or LED (3b) if
the refrigeration is active, or no LED if the actual temperature is equal to the set-point.
In Display 1 of the controller the actual temperature value is displayed.
•With inactive week program timer:
In Display 2 of the controller the actual date and time are displayed. Example:
15.05.06 13:52
•With active week program timer:
In Display 2 of the controller the actual date and time and the states of the week program timer
channels are displayed. Examples:
15.05.06 13:52 - - 15.05.06 13:52 - �
15.05.06 13:52 �- 15.05.06 13:52 ��
Channel 1 Off,
Channel 2: Off
Channel 1: Off,
Channel 2: On
Channel 1: On,
Channel 2: Off
Channel 1 On,
Channel 2: On
Display 1
Display 2
Figure 6: RD3 program controller

KB (E3.1) 08/2012 page 20/84
LED function indications and their signification:
(3a)
(yellow)
Heating active
(3b) (yellow) Refrigeration active
(3c) (yellow) No function
(3d) (green)
Illuminates: program operation
Flashes: exceeding of the tolerance limits in Fixed value entry mode or in
Program operation. In program operation: program interruption.
The program controller RD3 permits programming of temperature cycles. For each program section also
the fan speed can be regulated.
Two programs with up to 10 sections each or one program with up to 20 sections can be entered (setting
in the user level, chap. 10).
When changing from 2 programs to 1 program or vice-versa, existing programs are deleted
The maximum length of an individual program section can be set to either 99 hs 59 min or to 999 hs 59
min (setting in the user level, chap. 10). This setting is then valid for all program sections.
Programming can be done directly via the controller keyboard or graphically at the computer using the
communication software APT-COM™ 3 DataControlSystem (option, chap. 16.1) specially developed by
BINDER.
5.2 General indications
The program controller RD3 offers several functional levels:
Normal Display / fixed value operation:
•Display of the actual value of temperature (display 1) and of the actual date and time (display 2).
•The chamber is in fixed value operating mode, equilibrating to the entered set-points.
Fixed value entry mode (chap. 6)
•Entry of set-points for temperature, fan speed, and the safety controller for fixed value operating mode
•Entry of temperature set-points SP 1 and SP 2 for week program operation
Program editor (chap. 8)
•Two programs with up to 10 sections each or one program with up to 20 sections can be entered
(selection in the user level, chap. 10). Entry of set-points for temperature and fan speed in all program
sections (chap. 8.2).
•Deleting a program section (chap. 8.4)
Program start level (chap. 9)
•Selection of an entered program
•Entry of settings affecting the program course, as start delay time or number of program cycles
•Program start
Week program editor (chap.7)
•Setting the shift points