Binder KB User manual

Issue 10/2021 Art. No. 7001-0307
Operating Manual
Translation of the original operating manual
KB / KB-UL (E4), KB / KB-UL (E6)
Cooling Incubators
with compressor technology and program control
Model Model version Art. No.
KB 53 (E4) KB053-230V 9020-0199, 9120-0199
KB 53-UL (E4) KB053UL-120V 9020-0302, 9120-0302
KB 115 (E4) KB115-230V 9020-0397, 9120-0397
KB 115-UL (E4) KB115UL-120V 9020-0398, 9120-0398
KB 240 (E6) KB240-230V 9020-0202, 9120-0202
KB 240-UL (E6) KB240UL-120V 9020-0304, 9120-0304
KB 400 (E6) KB400-230V 9020-0203, 9120-0203
KB 400-UL (E6) KB400UL-120V 9020-0305, 9120-0305
KB 720 (E6) KB720-230V 9020-0204, 9120-0204
KB 720-UL (E6) KB720UL-240V 9020-0306, 9120-0306
BINDER GmbH
Address: Post office box 102, 78502 Tuttlingen, Germany Phone: [phone]
Fax: [phone] Internet: http://www.binder-world.com
E-mail: [e-mail] Service Hotline: [phone]
Service Fax: [phone] Service E-Mail: [e-mail]
Service Hotline USA: [phone] or [phone] x3
Service Hotline Asia Pacific: [phone] or [phone]
Service Hotline Russia and CIS: [phone]

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Contents
1. SAFETY.................................................................................................................. 6
1.1 Personnel Qualification .......................................................................................................................6
1.2 Operating manual................................................................................................................................6
1.3 Legal considerations ...........................................................................................................................6
1.3.1 Intellectual property...................................................................................................................7
1.4 Structure of the safety instructions......................................................................................................7
1.4.1 Signal word panel .....................................................................................................................7
1.4.2 Safety alert symbol ...................................................................................................................8
1.4.3 Pictograms ................................................................................................................................8
1.4.4 Word message panel structure.................................................................................................9
1.5 Localization / position of safety labels on the chamber ......................................................................9
1.6 Type plate..........................................................................................................................................10
1.7 General safety instructions on installing and operating the chambers .............................................11
1.8 Intended use .....................................................................................................................................13
1.9 Foreseeable Misuse..........................................................................................................................14
1.10 Residual Risks ..................................................................................................................................15
1.11 Operating instructions .......................................................................................................................16
1.12 Measures to prevent accidents .........................................................................................................16
2. CHAMBER DESCRIPTION .................................................................................. 18
2.1 Chamber overview ............................................................................................................................19
2.2 Instrument panel ...............................................................................................................................20
2.3 Lateral control panel with main power switch and optional equipment –KB / KB-UL 240 / 400 / 720
(E6)....................................................................................................................................................21
2.4 Chamber rear – KB / KB-UL 53 / 115 (E4)........................................................................................22
3. COMPLETENESS OF DELIVERY, TRANSPORTATION, STORAGE, AND
INSTALLATION.................................................................................................... 23
3.1 Unpacking, and checking equipment and completeness of delivery ................................................23
3.2 Guidelines for safe lifting and transportation.....................................................................................24
3.3 Storage..............................................................................................................................................24
3.4 Location of installation and ambient conditions ................................................................................25
4. INSTALLATION OF THE CHAMBER .................................................................. 27
4.1 Spacers for wall distance – KB / KB-UL 240 / 400 / 720 (E6)...........................................................27
4.2 Mounting the flexible tilt protection kit...............................................................................................28
4.3 Electrical connection .........................................................................................................................29
5. START UP ............................................................................................................ 30
6. FUNCTIONAL OVERVIEW OF THE T4.12 CHAMBER CONTROLLER ............. 30
6.1 Menu structure ..................................................................................................................................31
6.1.1 General menu .........................................................................................................................31
6.1.2 Quick menu.............................................................................................................................33
6.1.3 “User” menu ............................................................................................................................33
6.2 Operating modes...............................................................................................................................34
6.2.1 Activating the “control off”mode or change to “fixed value” operating mode.........................34
6.3 Performance during and after power failure......................................................................................36
6.4 Information ........................................................................................................................................36
7. CONFIGURATION OF OPTIONAL EQUIPMENT................................................ 37
7.1 Switching on or off the optional zero-voltage relay control outputs ..................................................38
7.2 Functional test of the optional alarm output ......................................................................................39
7.3 Switching on or off the optional object temperature display .............................................................39

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8. SET-POINT ENTRY IN “FIXED VALUE” OPERATING MODE ........................... 40
8.1 Set-point ranges................................................................................................................................40
8.2 Entering the set-points via “quick menu”...........................................................................................40
8.3 Entering the set-points via general menu .........................................................................................42
9. TIME PROGRAMS ............................................................................................... 43
9.1 Starting and running an existing time program .................................................................................45
9.2 Cancelling a running time program ...................................................................................................48
9.3 Creating a new time program............................................................................................................49
9.3.1 Section handling .....................................................................................................................51
9.3.2 Temperature setpoint..............................................................................................................52
9.3.3 Section duration ......................................................................................................................52
9.3.4 Repeating one or several sections within a time program......................................................53
9.3.5 Tolerance range......................................................................................................................54
9.3.6 Set-point ramp and set-point step modes...............................................................................56
9.3.7 Switching on or off the optional zero-voltage relay outputs ....................................................58
9.3.8 Calling up the next parameter.................................................................................................58
9.3.9 Saving the time program and leaving the program editor.......................................................60
9.4 Program interruption .........................................................................................................................61
9.5 Deleting a time program....................................................................................................................63
10. WEEK PROGRAMS ............................................................................................. 64
10.1 Starting and running an existing week program................................................................................65
10.2 Cancelling a running week program .................................................................................................67
10.3 Creating a new week program ..........................................................................................................68
10.3.1 Section handling .....................................................................................................................70
10.3.2 Temperature setpoint..............................................................................................................71
10.3.3 Day of the week ......................................................................................................................71
10.3.4 Time of the day .......................................................................................................................72
10.3.5 Activity of the shift-point..........................................................................................................73
10.3.6 Switching on or off the optional zero-voltage relay outputs....................................................73
10.3.7 Calling up the next parameter.................................................................................................74
10.3.8 Saving the week program and leaving the program editor.....................................................76
10.4 Deleting a week program ..................................................................................................................77
11. KEY LOCK ........................................................................................................... 78
11.1 Directly activating the key lock function ............................................................................................79
11.2 Automatic key lock ............................................................................................................................79
11.3 Changing the password for unlocking the key lock...........................................................................81
12. GENERAL CONTROLLER SETTINGS................................................................ 82
12.1 Setup wizard .....................................................................................................................................83
12.2 Date and time settings ......................................................................................................................83
12.3 Selecting the menu language of the T4.12 controller .......................................................................85
12.4 Setting display brightness .................................................................................................................85
12.5 Changing the temperature unit..........................................................................................................86
12.6 Defining the data recording rate........................................................................................................86
12.7 Factory reset .....................................................................................................................................87
12.8 Network configuration .......................................................................................................................87
12.9 Display of the entire network configuration .......................................................................................91
12.10 Display and entry of the chamber configuration – for service purpose.............................................91
13. DATA TRANSFER VIA USB INTERFACE........................................................... 92
13.1 Exporting data to USB drive..............................................................................................................92
13.2 Importing data from USB drive..........................................................................................................93

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14. NOTIFICATIONS AND ALARMS ......................................................................... 94
14.1 Notifications overview .......................................................................................................................94
14.2 Alarms overview................................................................................................................................94
14.3 Alarm status ......................................................................................................................................95
14.4 Confirming a “set” alarm....................................................................................................................96
14.5 Alarm configuration and overview.....................................................................................................97
14.5.1 List of active alarms ................................................................................................................97
14.5.2 History – list of all alarms........................................................................................................98
14.5.3 Activating, deactivating, and testing the alarm buzzer ...........................................................99
14.5.4 Activating / deactivating all alarm functions............................................................................99
15. EVENT LIST ....................................................................................................... 100
16. GRAPHICAL DISPLAY OF THE MEASURED VALUES ................................... 101
16.1 Setting the sampling rate ................................................................................................................101
16.2 Defining the display range...............................................................................................................102
16.3 Selecting the parameters ................................................................................................................103
17. TEMPERATURE SAFETY DEVICES................................................................. 103
17.1 Over temperature protective device (class 1) .................................................................................103
17.2 Overtemperature safety controller (temperature safety device class 3.1) ......................................103
17.2.1 Safety controller modes ........................................................................................................104
17.2.2 Setting the safety controller ..................................................................................................104
17.3 Over- and undertemperature safety controller (temperature safety device class 3.3) (option) ......108
17.3.1 Safety controller modes ........................................................................................................108
17.3.2 Setting the Over- and undertemperature safety controller ...................................................109
18. DEFROSTING DURING REFRIGERATING OPERATION ................................ 112
19. OPTIONS............................................................................................................ 113
19.1 APT-COM™ 4 Multi Management Software (option)......................................................................113
19.2 Data logger kit (option)....................................................................................................................113
19.3 Interior lighting.................................................................................................................................113
19.4 Zero-voltage relay alarm output (option).........................................................................................113
19.5 Zero-voltage relay control outputs (may be available via BINDER INDIVIDUAL Customized
Solution) ..........................................................................................................................................114
19.6 Object temperature display with flexible Pt 100 temperature sensor (option) ................................115
20. CLEANING AND DECONTAMINATION ............................................................ 116
20.1 Cleaning ..........................................................................................................................................116
20.2 Decontamination / chemical disinfection.........................................................................................117
21. MAINTENANCE AND SERVICE, TROUBLESHOOTING, REPAIR, TESTING. 119
21.1 General information, personnel qualification...................................................................................119
21.2 Maintenance intervals, service........................................................................................................119
21.3 Simple troubleshooting....................................................................................................................120
21.4 Sending the chamber back to BINDER GmbH ...............................................................................123
22. DISPOSAL.......................................................................................................... 123
22.1 Disposal of the transport packing....................................................................................................123
22.2 Decommissioning............................................................................................................................124
22.3 Disposal of the chamber in the Federal Republic of Germany .......................................................124
22.4 Disposal of the chamber in the member states of the EU except for the Federal Republic of
Germany..........................................................................................................................................125
22.5 Disposal of the chamber in non-member states of the EU .............................................................126

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23. TECHNICAL DESCRIPTION.............................................................................. 127
23.1 Factory calibration and adjustment .................................................................................................127
23.2 Over current protection ...................................................................................................................127
23.3 Definition of usable volume.............................................................................................................127
23.4 Technical data.................................................................................................................................128
23.5 Equipment and Options (extract) ....................................................................................................130
23.6 Spare parts and accessories (extract) ............................................................................................131
23.7 KB / KB-UL 53 dimensions..............................................................................................................132
23.8 KB / KB-UL 115 dimensions............................................................................................................133
23.9 KB / KB-UL 240 dimensions............................................................................................................134
23.10 KB / KB-UL 400 dimensions............................................................................................................135
23.11 KB / KB-UL 720 dimensions............................................................................................................136
24. CERTIFICATES AND DECLARATIONS OF CONFORMITY............................. 137
24.1 EU Declaration of Conformity..........................................................................................................137
24.2 Certificate for the GS mark of conformity of the “Deutsche Gesetzliche Unfallversicherung e.V.“
(German Social Accident Insurance) DGUV...................................................................................140
25. CONTAMINATION CLEARANCE CERTIFICATE ............................................. 142
25.1 For chambers located outside USA and Canada ...........................................................................142
25.2 For chambers in USA and Canada .................................................................................................145

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Dear customer,
For the correct operation of the chambers, it is important that you read this operating manual completely
and carefully and observe all instructions as indicated. Observe the national regulations on minimum age
of laboratory personnel. Failure to read, understand and follow the instructions may result in personal injury.
It can also lead to damage to the chamber and/or poor equipment performance.
1. Safety
1.1 Personnel Qualification
The chamber must only be installed, tested, and started up by personnel qualified for assembly, startup,
and operation of the chamber. Qualified personnel are persons whose professional education, knowledge,
experience and knowledge of relevant standards allow them to assess, carry out, and identify any potential
hazards in the work assigned to them. They must have been trained and instructed, and be authorized, to
work on the chamber.
The chamber should only be operated by laboratory personnel especially trained for this purpose and fa-
miliar with all precautionary measures required for working in a laboratory. Observe the national regulations
on minimum age of laboratory personnel.
1.2 Operating manual
This operating manual is part of the components of delivery. Always keep it handy for reference in the
vicinity of the chamber. If selling the unit, hand over the operating manual to the purchaser.
To avoid injuries and damage observe the safety instructions of the operating manual. Failure to follow
instructions and safety precautions can lead to significant risks.
DANGER
Dangers due to failure to observe the instructions and safety precautions.
Serious injuries and chamber damage. Risk of death.
Observe the safety instructions in this Operating Manual.
Follow the operating procedures in this Operating Manual.
Carefully read the complete operating instructions of the chamber prior to installing and
using the chamber.
Keep the operating manual for future reference
Make sure that all persons who use the chamber and its associated work equipment have
read and understood the Operating Manual.
This Operating Manual is supplemented and updated as needed. Always use the most recent version of
the Operating Manual. When in doubt, call the BINDER Service Hotline for information on the up-to-date-
ness and validity of this Operating Manual.
1.3 Legal considerations
This operating manual is for informational purposes only. It contains information for correct and safe in-
stalling, start-up, operation, decommissioning, cleaning and maintenance of the product. Note: the contents
and the product described are subject to change without notice.
Understanding and observing the instructions in this operating manual are prerequisites for hazard-free use
and safety during operation and maintenance. Images are to provide basic understanding. They may devi-
ate from the actual version of the chamber. The actual scope of delivery can, due to optional or special
design, or due to recent technical changes, deviate from the information and illustrations in these instruc-
tions this operating manual. In no event shall BINDER be held liable for any damages, direct or incidental
arising out of or related to the use of this manual.

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This operating manual cannot cover all conceivable applications. If you would like additional information,
or if special problems arise that are not sufficiently addressed in this manual, please ask your dealer or
contact us directly, e.g. by phone at the number located on page one of this manual
Furthermore, we emphasize that the contents of this operating manual are not part of an earlier or existing
agreement, description, or legal relationship, nor do they modify such a relationship. All obligations on the
part of BINDER derive from the respective purchase contract, which also contains the entire and exclusively
valid statement of warranty administration and the general terms and conditions, as well as the legal regu-
lations valid at the time the contract is concluded. The statements in this manual neither augment nor
restrict the contractual warranty provisions.
1.3.1 Intellectual property
Trademark Information: All BINDER trademarks relating to products or service, as well as trade names,
logos and product names used on the website, products and documents of BINDER company are trade-
marks or registered trademarks of BINDER company (including BINDER GmbH, BINDER Inc.) in the U.S.
and other countries and communities of states. This includes word marks, position marks, word/figurative
marks, design configurations, figurative marks, and design patents.
Patent Information: BINDER products, categories of products, and accessories may be covered by one
or more patents and/or utility models in the U.S. and other countries and communities of states. Additional
patent applications may also be pending in the U.S. and other countries and communities of states.
Please visit www.binder-world.com for more information.
1.4 Structure of the safety instructions
In this operating manual, the following safety definitions and symbols indicate dangerous situations follow-
ing the harmonization of ISO 3864-2 and ANSI Z535.6.
1.4.1 Signal word panel
Depending on the probability of serious consequences, potential dangers are identified with a signal word,
the corresponding safety color, and if appropriate, the safety alert symbol.
DANGER
Indicates an imminently hazardous situation that, if not avoided, will result in death or serious (irreversi-
ble) injury.
WARNING
Indicates a potentially hazardous situation which, if not avoided, could result in death or serious (irre-
versible) injury.
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor (re-
versible) injury.
NOTICE
Indicates a potentially hazardous situation which, if not avoided, may result in damage to the product
and/or its functions or of a property in its proximity.

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1.4.2 Safety alert symbol
Use of the safety alert symbol indicates a risk of injury.
Observe all measures that are marked with the safety alert symbol in order to avoid death or
injury.
1.4.3 Pictograms
Warning signs
Electrical hazard
Hot surface
Explosive atmosphere
Stability hazard
Lifting hazard
Risk of corrosion and /
or chemical burns
Harmful substances
Biohazard
Pollution Hazard
Mandatory action signs
Mandatory regulation
Read operating
instructions
Disconnect the power
plug
Lift with several persons
Lift with mechanical
assistance
Environment protection
Wear protective gloves
Wear safety goggles
Prohibition signs
Do NOT touch
Do NOT spray with
water
Do NOT climb
Information to be observed in order to ensure optimum function of the product.

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1.4.4 Word message panel structure
Type / cause of hazard.
Possible consequences.
∅Instruction how to avoid the hazard: prohibition
Instruction how to avoid the hazard: mandatory action
Observe all other notes and information not necessarily emphasized in the same way, in order to avoid
disruptions that could result in direct or indirect injury or property damage.
1.5 Localization / position of safety labels on the chamber
The following labels are located on the chamber:
Pictograms (Warning signs)
Hot surface
•Inner glass door above the glass door
handle
Read operating manual
•UL chambers: outer chamber door
Service label
Figure 1: Position of labels on the chamber (example KB 240-UL)
Keep safety labels complete and legible.
Replace safety labels that are no longer legible. Contact BINDER Service for these replacements.

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1.6 Type plate
The type plate sticks to the left side of the chamber, bottom right-hand.
Figure 2: Type plate (example of KB 240 regular chamber)
Figure 3: Type plate (example of KB 240-UL regular chamber)
Indications of the type plate (example)
Indication
Information
BINDER
Manufacturer: BINDER GmbH
KB 240
Model designation
Cooling incubator
Device name
Serial No.
00000000000000
Serial no. of the chamber
Built
2021
Year of construction
Nominal temperature
100 °C
212 °F
Nominal temperature
IP protection
20
IP type of protection acc. to standard EN 60529
Temp. safety device DIN 12880
Temperature safety device acc. to standard DIN
12880:2007
Class
3.1
Class of temperature safety device
Art. No.
9020-0202
Art. no. of the chamber
Project No.
---
Optional: Special application acc. to project no.
1,20 kW
Nominal power
5,2 A
Nominal current
200-230 V / 50 Hz
Nominal voltage range +/-10%
at the indicated power frequency
200-230 V / 60 Hz
1 N ~
Current type
Max. operating pressure 15 bar
Max. operating pressure in the refrigerating system
R 134A - 0,35 kg
Refrigerant type and max. filling weight
Contains fluorinated greenhouse gases
covered by the Kyoto Protocol
Contains fluorinated greenhouse gases covered by the
Kyoto Protocol
Nominal temp.
100 °C
1,20 kW / 5,2 A
Max. operating pressure 15 bar
212 °F
200-230 V / 50 Hz
R 134A – 0,35 kg
IP protection
20
200-230 V / 60 Hz
Contains fluorinated
Safety device
DIN 12880
1 N ~
greenhouse gases covered
Class
3.1
by the Kyoto Protocol
Art. No.
9020-0202
Project No.
Built
2021
Cooling incubator
BINDER GmbH
Im Mittleren Ösch 5
78532 Tuttlingen / Germany
www.binder-world.com
KB 240
E6
Serial No. 00000000000000
Made in Germany
Nominal temp.
100 °C
1,20 kW / 10,0 A
Max. operating pressure 15 bar
212 °F
100-120 V / 50 Hz
R 134A – 0,24 kg
IP protection
20
100-120 V / 60 Hz
Contains fluorinated
Safety device
DIN 12880
1 N ~
greenhouse gases covered
Class
3.1
by the Kyoto Protocol
Art. No.
9020-0304
Project No.
Built
2021
Cooling incubator
BINDER GmbH
Im Mittleren Ösch 5
78532 Tuttlingen / Germany
www.binder-world.com
KB 240-UL
E6
Serial No. 00000000000000
Made in Germany

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Symbols on the type plate
Symbol Valid for Information
All chambers CE conformity marking
All chambers
Electrical and electronic equipment manufactured / placed on
the market in the EU after 13 August 2005 and be disposed
of in separate collection according to Directive 2012/19/EU
on waste electrical and electronic equipment (WEEE).
Not for UL chambers
GS mark of conformity of the “Deutsche Gesetzliche Unfall-
versicherung e.V. (DGUV), Prüf- und Zertifizierungsstelle
Nahrungsmittel und Verpackung im DGUV Test” (German
Social Accident Insurance (DGUV), Testing and Certification
Body for Foodstuffs and Packaging Industry in DGUV Test).
Not for UL chambers
The chamber is certified according to Customs Union Tech-
nical Regulation (CU TR) for the Eurasian Economic Union
(Russia, Belarus, Armenia, Kazakhstan Kyrgyzstan).
UL chambers only
The chamber is certified by Underwriters Laboratories Inc.®
according to the following standards:
•UL 61010-1, 3rd Edition, 2012-05, Rev. 2015-07
•CAN/CSA-C22.2 No. 61010-1, 3rd Edition, 2012-05, Rev.
2015-07
•IEC/EN 61010-1:2014, 3rd Edition
1.7 General safety instructions on installing and operating the chambers
With regard to operating the chambers and to the installation location, please observe the local and national
regulations relevant for your country (for Germany: DGUV guidelines 213-850 on safe working in laborato-
ries, issued by the employers’ liability insurance association).
BINDER GmbH is only responsible for the safety features of the chamber provided skilled electricians or
qualified personnel authorized by BINDER perform all maintenance and repair, and if components relating
to chamber safety are replaced in the event of failure with original spare parts.
To operate the chamber, use only original BINDER accessories or accessories from third-party suppliers
authorized by BINDER. The user is responsible for any risk caused by using unauthorized accessories.
NOTICE
Danger of overheating due to lack of ventilation.
Damage to the chamber.
∅Do NOT install the chamber in unventilated recesses.
Ensure sufficient ventilation for dispersal of the heat.
Observe the prescribed minimum distances when installing the chamber (chap. 3.4)
LABORATORY EQUIPMENT
43KM

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Do not install or operate the chambers in hazardous locations.
DANGER
Danger of explosion due to combustible dusts or explosive mixtures in the vicinity
of the chamber.
Serious injury or death from burns and / or explosion pressure.
∅Do NOT operate the chamber in potentially explosive areas.
KEEP combustible dust or air-solvent mixtures AWAY from the chamber.
The chambers do not dispose of any measures of explosion protection.
DANGER
Danger of explosion due to introduction of flammable or explosive substances in
the chamber.
Serious injury or death from burns and / or explosion pressure.
∅Do NOT introduce any substance into the chamber which is combustible or explosive at
working temperature.
∅Do NOT introduce any combustible dust or air-solvent mixture in the inner chamber.
Any solvent contained in the charging material must not be explosive or inflammable. I.e., irrespective of
the solvent concentration in the steam room, NO explosive mixture with air must form. The temperature
inside the chamber must lie below the flash point or below the sublimation point of the charging material.
Familiarize yourself with the physical and chemical properties of the charging material, as well as the con-
tained moisture constituent and its behavior with the addition of heat energy.
Familiarize yourself with any potential health risks caused by the charging material, the contained moisture
constituent or by reaction products which may arise during the temperature process. Take adequate
measures to exclude such risks prior to putting the cooling incubator into operation.
DANGER
Electrical hazard by water entering the chamber.
Deadly electric shock.
∅The chamber must NOT become wet during operation, cleaning, or maintenance.
∅Do NOT install the chamber in damp areas or in puddles.
Set up the chamber in a way that it is splash-proof.
The chambers were produced in accordance with VDE regulations and were routinely tested in accordance
to VDE 0411-1 (IEC 61010-1).
During and shortly after operation, the temperature of the inner surfaces almost equals the set-point. The
glass doors, the glass door handles, and the inner chamber will become hot during operation.
CAUTION
Danger of burning by touching hot chamber parts during operation.
Burns.
∅Do NOT touch the glass doors, the glass door handles, the inner surfaces or the charg-
ing material during operation.

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WARNING
Danger of injury and damages by the chamber tipping over or breakaway of the pro-
truding lower housing cover.
Injuries and damage to the chamber and the loading material
∅Do NOT load the lower housing cover with heavy objects while the chamber door is
open and do NOT climb on it.
1.8 Intended use
Following the instructions in this operating manual and conducting regular maintenance work
(chap. 21.2) are part of the intended use.
Any use of the chambers that does not comply with the requirements specified in this Operating
Manual shall be considered improper use.
Other applications than those described in this chapter are not approved.
Use
Cooling incubators KB / KB-UL are suitable for exact conditioning of harmless materials. Because of their
precise temperature accuracy these devices are especially useful for cultivation of microorganisms with a
narrow temperature optimum in a range of 4 °C / 39.2 °F to 37 °C / 98.6 °F. Main fields of application are
tests of long-term storage (e.g. at 4 °C / 39.2 °F), refrigerated incubation between 20 °C / 68 °F and 25 °C
/ 77 °F and incubation at 37 °C / 98.6 °F (also with additional introduction of heat) or with alternating tem-
peratures (e.g. 37 °C / 98.6 °F and 4 °C / 39.2 °F).
Requirements for the chamber load
Any solvent must not be explosive and flammable. A mixture of any component of the charging material
with air must NOT be explosive. The operating temperature must lie below the flash point or below the
sublimation point of the charging material. Any component of the charging material must NOT be able to
release toxic gases.
The charging material shall not contain any corrosive ingredients that may damage the machine compo-
nents made of stainless steel, aluminum, and copper. Such ingredients include in particular acids and hal-
ides. Any corrosive damage caused by such ingredients is excluded from liability by BINDER GmbH.
The chamber does not dispose of any measures of explosion protection.
DANGER
Explosion or implosion hazard and danger of poisoning through the introduction of
unsuitable loading material.
Poisoning. Serious injury or death from burns and / or explosion pressure.
∅Do NOT introduce any substance combustible or explosive at working temperature into
the chamber, in particular no energy sources such as batteries or lithium-ion batteries.
∅NO explosive dust or air-solvent mixture in the inner chamber.
∅Do NOT introduce any substance which could lead to release of toxic gases.

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Contamination of the chamber by toxic, infectious or radioactive substances must be prevented
WARNING
Danger of intoxication and infection through contamination of the chamber with
toxic, infectious or radioactive substances.
Damages to health.
Protect the interior of the chamber from contamination by toxic, infectious or radioactive
substances.
Take suitable protective measures when introducing and removing toxic, infectious or
radioactive material
In case of foreseeable use of the chamber there is no risk for the user through the integration of the chamber
into systems or by special environmental or operating conditions in the sense of EN 61010-1:2010. For this,
the intended use of the chamber and all its connections must be observed.
Medical devices
The chambers are not classified as medical devices as defined by the Medical Device Directive 93/42/EEC
and Regulation (EU) No 2017/745.
Due to the special demands of the Medical Device Directive (MDD), these chambers are not
qualified to perform sterilization of medical devices as defined by the directive 93/42/EWG.
Personnel Requirements
Only trained personnel with knowledge of the Operating Manual can set up and install the chamber, start it
up, operate, clean, and take it out of operation. Service and repairs call for further technical requirements
(e.g. electrical know-how), as well as knowledge of the service manual.
Installation site requirements
The chambers are designed for setting up inside a building (indoor use).
The requirements described in the Operating Manual for installation site and ambient conditions (Chap.
3.4) must be met.
WARNING: If customer should use a BINDER chamber running in non-supervised continu-
ous operation, we strongly recommend in case of inclusion of irrecoverable specimen or
samples to split such specimen or samples and store them in at least two chambers, if this is
feasible.
1.9 Foreseeable Misuse
Other applications than those described in chap. 1.8 are not approved.
This expressly includes the following misuses (the list is not exhaustive), which pose risks despite the
inherently safe construction and existing technical safety equipment:
•Non-observance of Operating Manual
•Non-observance of information and warnings on the chamber (e.g. control unit messages, safety iden-
tifiers, warning signals)
•Installation, startup, operation, maintenance and repair by untrained, insufficiently qualified, or unau-
thorized personnel
•Missed or delayed maintenance and testing
•Non-observance of traces of wear and tear
•Insertion of materials excluded or not permitted by this Operating Manual.
•Non-compliance with the admissible parameters for processing the respective material.

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•Installation, testing, service or repair in the presence of solvents
•Installation of replacement parts and use of accessories and operating resources not specified and au-
thorized by the manufacturer
•Installation, startup, operation, maintenance or repair of the chamber in absence of operating instruc-
tions
•Bypassing or changing protective systems, operation of the chamber without the designated protective
systems
•Non-observance of messages regarding cleaning and disinfection of the chamber.
•Spilling water or cleaning agent on the chamber, water penetrating into the chamber during operation,
cleaning or maintenance.
•Cleaning activity while the chamber is turned on.
•Operation of the chamber with a damaged housing or damaged power cord
•Continued operation of the chamber during an obvious malfunction
•Insertion of objects, particularly metallic objects, in louvers or other openings or slots on the chamber
•Human error (e.g. insufficient experience, qualification, stress, exhaustion, laziness)
To prevent these and other risks from incorrect operation, the operator shall issue operating instructions.
Standard operating procedures (SOPs) are recommended.
1.10 Residual Risks
The unavoidable design features of a chamber, as well as its proper field of application, can also pose risks,
even during correct operation. These residual risks include hazards which, despite the inherently safe de-
sign, existing technical protective equipment, safety precautions and supplementary protective measures,
cannot be ruled out.
Messages on the chamber and in the Operating Manual warn of residual risks. The consequences of these
residual risks and the measures required to prevent them are listed in the Operating Manual. Moreover, the
operator must take measures to minimize hazards from unavoidable residual risks. This includes, in partic-
ular, issuing operating instructions.
The following list summarizes the hazards against which this Operating Manual and the Service Manual
warn, and specifies protective measures at the appropriate spots:
Unpacking, Transport, Installation
•Sliding or tilting the chamber
•Setup of the chamber in unauthorized areas
•Installation of a damaged chamber
•Installation of a chamber with damaged power cord
•Inappropriate site of installation
•Missing protective conductor connection
Normal operation
•Assembly errors
•Contact with hot surfaces on the housing
•Contact with hot surfaces in the interior and inside of doors
•Emission of non-ionizing radiation from electrical operating resources
•Contact with live parts in normal state

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Cleaning and Decontamination
•Penetration of water into the chamber
•Inappropriate cleaning and decontamination agents
•Enclosure of persons in the interior
Malfunction and Damage
•Continued operation of the chamber during an obvious malfunction or outage of the heating or cooling
system
•Contact with live parts during error status
•Operation of a unit with damaged power cord
Maintenance
•Maintenance work on live parts.
•Execution of maintenance work by untrained/insufficiently qualified personnel
•Electrical safety analysis during annual maintenance not performed
Trouble-shooting and Repairs
•Non-observance of warning messages in the Service Manual
•Trouble-shooting of live parts without specified safety measures
•Absence of a plausibility check to rule out erroneous inscription of electrical components
•Performance of repair work by untrained/insufficiently qualified personnel
•Inappropriate repairs which do not meet the quality standard specified by BINDER
•Use of replacement parts other than BINDER original replacement parts
•Electrical safety analysis not performed after repairs
1.11 Operating instructions
Depending on the application and location of the chamber, the operator of the chamber must provide the
relevant information for safe operation of the chamber in a set of operating instructions.
Keep these operating instructions with the chamber at all times in a place where they are
clearly visible. They must be comprehensible and written in the language of the employees.
1.12 Measures to prevent accidents
The operator of the chamber must observe the local and national regulations (for Germany: the rule “Op-
eration of work equipment. Operation of refrigeration systems, heat pumps and refrigeration equipment”,
GUV-R 500 chap. 2.35) and take precautions to prevent accidents.
The manufacturer took the following measures to prevent ignition and explosions:
•Indications on the type plate
See operating manual chap. 1.6.
•Operating manual
An operating manual is available for each chamber.

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•Overtemperature monitoring
The chamber is equipped with a temperature display, which can be read from outside.
The chamber is equipped with an additional safety controller (temperature safety device class 3.1 acc.
to DIN 12880:2007). Visual and audible (buzzer) signals indicate temperature exceeding.
•Safety, measurement, and control equipment
The safety, measuring, and control equipment is easily accessible.
•Electrostatic charge
The interior parts are grounded.
•Non-ionizing radiation
Non-ionizing radiation is not intentionally produced, but released only for technical reasons by electrical
equipment (e.g. electric motors, power cables, solenoids). The machine has no permanent magnets. If
persons with active implants (e.g. pacemakers, defibrillators) keep a safe distance (distance of field
source to implant) of 30 cm, an influence of these implants can be excluded with high probability.
•Protection against touchable surfaces
Tested according to EN ISO 13732-1:2008.
•Floors
See operating manual chap. 3.4 for correct installation
•Cleaning
See operating manual chap. 20.
•Examinations
The chamber has been inspected by the “Deutsche Gesetzliche Unfallversicherung e.V. (DGUV) (Ger-
man Social Accident Insurance (DGUV)” (German Social Accident Insurance (DGUV), Testing and Cer-
tification Body for Foodstuffs and Packaging Industry in DGUV Test) and bears the GS mark. (Not valid
for UL chambers)
UL units only: The chamber is certified by Underwriters Laboratories Inc.®according to the following
standards: UL 61010-1, 3rd Edition, 2012-05, Rev. 2015-07; CAN/CSA-C22.2 No. 61010-1, 3rd Edition,
2012-05, Rev. 2015-07; IEC/EN 61010-1:2014, 3rd Edition

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2. Chamber description
A high level of precision, reliability, and safety for all growth parameters ensures optimum incubation con-
ditions. Moreover, the KB / KB-UL cooling incubator is designed for maximum usability – even in continuous
operation year after year. It fulfills all technical and application-specific requirements arising in experimen-
tation such as in the areas of biotechnology, medicine, the nutrition industry, pharmaceutical and cosmetics
industries, botany, and zoology.
Two important temperature technologies have been combined to achieve perfect temperature control. The
specially developed DCT™ refrigerating system, a direct refrigerating process, in conjunction with the
APT.line™ preheating chamber technology, satisfies the unique prerequisites for attaining highly-precise
temperature control and particularly short recovery times after opening the door.
The refrigerating system is distinguished by direct, precise, and rapid temperature conduction. Large-area
labyrinth evaporator plates directly conduct the cold to the atmosphere of the working space.
The APT.line™ preheating chamber system ensures high level of spatial and time-based temperature pre-
cision, thanks to the direct and distributed air circulation into the interior. This is especially important for
maintaining temperatures – especially with full chambers – and for rapid restoration of optimum growth
conditions after opening the door. The inner glass door ensures that the temperature remains constant
when observing the incubation process. The fan supports exact attainment and maintenance of the desired
temperature accuracy. The fan speed is digitally adjustable. The heating and refrigerating systems are
microprocessor regulated to a tenth of a degree. In addition, the chamber provides almost unlimited possi-
bilities for adaptation to individual customer requirements based upon extensive programming options and
on the week program timer and real time clock of the controller.
The KB / KB-UL 240 / 400 / 720 (E6) chambers are equipped with a door heating.
Material: The inner chamber, the pre-heating chamber and the interior side of the doors are all made of
stainless steel V2A (German material no. 1.4301, US equivalent AISI 304). The housing is RAL 7035 pow-
der-coated. All corners and edges are also completely coated.
All chamber functions are easy and comfortable to use thanks to their clear arrangement. Major features
are easy cleaning of all chamber parts and avoidance of undesired contamination.
The chamber comes equipped with an Ethernet interface for computer communication, e.g. via the APT-
COM™ 4 Multi Management Software (option, chap. 19.1). For further options, see chap. 23.5.
The KB / KB-UL 240 / 400 / 720 (E6) chambers are equipped with four castors. Both front castors can easily
be locked via the attached brakes.
Temperature range at ambient temperature of +22 °C +/- 3°C / 71.6 °F +/- 5.4 °F: -10 °C / 14 °F up to
+100 °C / 212 °F

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2.1 Chamber overview
Instrument panel with microprocessor
controller T4.12 and USB interface
Door handle
Chamber door
Refrigerating module
Main power switch (1)
Figure 4: Cooling incubator KB / KB-UL 53 / 115 (E4)
Instrument panel with microproces-
sor controller T4.12 and USB inter-
face
Door handle
Chamber door
Refrigerating module
Lateral control panel with main power
switch and connections
Figure 5: Cooling incubator KB / KB-UL 240 / 400 / 720 (E6)

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2.2 Instrument panel
5,7" controller display
Context-sensitive buttons
USB interface
Operating button
Pilot lamp: ready for operation
Figure 6: Instrument panel with microprocessor controller T4.12 and USB interface