Binder CB-S 170 User manual

Issue 06/2020 Art. No. 7001-0402
Operating Manual
CB-S / CB-S-UL (E7)
CO2Incubator
with FPI-sensor system and RD4 controller
Model Model version Art. No.
CB-S 170 CBS170-230V 9040-0189, 9140-0189
CB-S 170-UL CBSUL170-120V 9040-0190, 9140-0190
CB-S 260 CBS260-230V 9040-0191, 9140-0191
CB-S 260-UL CBSUL260-120V 9040-0192, 9140-0192
BINDER GmbH
Address: Post office box 102, 78502 Tuttlingen, Germany Phone: [phone]
Fax: [phone] Internet: http://www.binder-world.com
E-mail: [e-mail] Service Hotline: [phone]
Service Fax: [phone] Service E-Mail: [e-mail]
Service Hotline USA: [phone] or [phone] x3
Service Hotline Asia Pacific: [phone] or [phone]
Service Hotline Russia and CIS: [phone]

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Contents
1. SAFETY..................................................................................................................6
1.1 Personnel Qualification.......................................................................................................................6
1.2 Operating manual................................................................................................................................6
1.3 Legal considerations...........................................................................................................................6
1.4 Structure of the safety instructions......................................................................................................7
1.4.1 Signal word panel .....................................................................................................................7
1.4.2 Safety alert symbol ...................................................................................................................7
1.4.3 Pictograms................................................................................................................................8
1.4.4 Word message panel structure.................................................................................................8
1.5 Localization / position of safety labels at the chamber .......................................................................9
1.6 Type plate..........................................................................................................................................10
1.7 General safety instructions on installing and operating the chamber...............................................11
1.8 Precautions when working with CO2gas..........................................................................................13
1.9 Precautions when handling gas cylinders.........................................................................................13
1.10 Intended use .....................................................................................................................................14
1.11 Foreseeable Misuse..........................................................................................................................15
1.12 Residual Risks ..................................................................................................................................16
1.13 Operating instructions .......................................................................................................................17
1.14 Measures to prevent accidents.........................................................................................................18
2. CHAMBER DESCRIPTION..................................................................................19
2.1 Chamber overview............................................................................................................................20
2.2 Inner chamber...................................................................................................................................21
2.3 Connection panel on the rear of the chamber...................................................................................22
2.4 Chamber doors .................................................................................................................................23
2.5 Instrument panel ...............................................................................................................................23
3. COMPLETENESS OF DELIVERY, TRANSPORTATION, STORAGE, AND
INSTALLATION....................................................................................................24
3.1 Unpacking, and checking equipment and completeness of delivery................................................24
3.2 Guidelines for safe lifting and transportation.....................................................................................25
3.3 Storage..............................................................................................................................................25
3.4 Location of installation and ambient conditions ................................................................................25
4. INSTALLATION AND CONNECTIONS ...............................................................28
4.1 Shelves..............................................................................................................................................28
4.2 CO2sensor........................................................................................................................................28
4.2.1 Connecting the CO2sensor ....................................................................................................28
4.2.2 General notes .........................................................................................................................28
4.3 Water pan..........................................................................................................................................29
4.4 Gas connection .................................................................................................................................30
4.4.1 Connecting the CO2gas cylinder............................................................................................31
4.4.2 Connecting the gas hose to the chamber rear .......................................................................32
4.4.3 Gas cylinder connection kit (option)........................................................................................33
4.5 Electrical connection.........................................................................................................................34
5. FUNCTIONAL OVERVIEW OF THE RD4 CHAMBER CONTROLLER...............35
5.1 Menu structure of the controller and access levels...........................................................................36
5.2 Performance during and after power failures and shut down...........................................................37
6. START UP............................................................................................................37
6.1 Turning on the chamber....................................................................................................................37
6.2 Preset factory parameters.................................................................................................................38
6.3 Performance after turning on the chamber.......................................................................................38
6.4 Altitude of the installation site above sea level .................................................................................39

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7. TEMPERATURE AND CO2SET-POINT ENTRY.................................................40
7.1 Temperature set-point entry..............................................................................................................40
7.2 CO2set-point entry............................................................................................................................40
8. PLACING SAMPLES IN THE INCUBATOR ........................................................41
9. SETTING SPECIAL CONTROLLER FUNCTIONS..............................................42
9.1 Idle mode...........................................................................................................................................42
9.2 Deactivated CO2control....................................................................................................................43
10. PASSWORD.........................................................................................................43
10.1 Password request .............................................................................................................................43
10.2 Assign and modify a password .........................................................................................................44
10.2.1 Assign and modify the User password ...................................................................................44
10.2.2 Assign and modify the Admin password.................................................................................44
11. TEMPERATURE SAFETY DEVICES...................................................................45
11.1 Over temperature protective device (class 1)...................................................................................45
11.2 Overtemperature safety controller class 3.1.....................................................................................45
11.2.1 Setting the safety controller mode..........................................................................................46
11.2.2 Setting the safety controller value...........................................................................................46
11.2.3 Message and measures in the state of alarm.........................................................................47
11.2.4 Function check........................................................................................................................47
12. GENERAL CONTROLLER SETTINGS................................................................48
12.1 Selecting the controller’s menu language.........................................................................................48
12.2 Selecting the temperature unit..........................................................................................................48
12.3 Setting the current date.....................................................................................................................49
12.4 Setting the current time.....................................................................................................................50
12.5 Function “Language selection at restart” ..........................................................................................50
12.6 Setting the chamber address............................................................................................................51
12.7 Display brightness.............................................................................................................................51
13. TOLERANCE RANGE SETTINGS.......................................................................52
13.1 Setting the delay time for tolerance range alarm..............................................................................52
13.2 Setting the temperature tolerance range ..........................................................................................52
13.3 Setting the CO2tolerance range.......................................................................................................53
13.4 Setting the delay time for door open alarm.......................................................................................53
14. CHAMBER SETTINGS (FOR EXPERIENCED USERS ONLY)...........................54
14.1 Setting the humidity control...............................................................................................................54
14.2 Setting the door heating offset..........................................................................................................54
14.3 Adjusting the heating power – for BINDER Service only..................................................................55
15. NOTIFICATION AND ALARM FUNCTIONS........................................................55
15.1 Alarm messages ...............................................................................................................................55
15.2 Information messages.......................................................................................................................57
15.3 Activating / deactivating the audible alarm (alarm buzzer)...............................................................58
15.4 Measures in case of alarm................................................................................................................58
15.4.1 Door open alarm.....................................................................................................................58
15.4.2 Safety controller temperature alarm .......................................................................................58
15.4.3 Temperature tolerance range alarm (overtemperature / too low temperature)......................59
15.4.4 CO2tolerance range alarm (CO2over/under concentration)..................................................60
15.4.5 CO2pressure alarm ................................................................................................................60
15.4.6 Power failure alarm.................................................................................................................61
15.4.7 Alarms referring to temperature sensor failures .....................................................................62
15.4.8 Alarms referring to CO2sensor failure....................................................................................63
15.4.9 Alarm on ineffective sterilization.............................................................................................63
15.5 Zero-voltage relay alarm output........................................................................................................64

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16. ETHERNET NETWORK SETTINGS....................................................................65
16.1 Showing the network settings ...........................................................................................................65
16.1.1 Showing the chamber‘s MAC address....................................................................................65
16.1.2 Showing the IP address..........................................................................................................65
16.1.3 Showing the subnet mask.......................................................................................................66
16.1.4 Showing the standard gateway...............................................................................................66
16.1.5 Showing the DNS server address...........................................................................................66
16.1.6 Showing the DNS chamber name ..........................................................................................67
16.2 Changing the configuration of the network settings..........................................................................67
16.2.1 Selecting the type of IP address assignment (automatic / manual) .......................................67
16.2.2 Selecting the type of assignment of the DNS server address (automatic / manual)..............68
16.2.3 Assigning the IP address........................................................................................................68
16.2.4 Setting the subnet mask .........................................................................................................69
16.2.5 Setting the standard gateway .................................................................................................69
16.2.6 Assigning the DNS server address.........................................................................................70
17. DATA RECORDER ..............................................................................................70
17.1 Recorded data...................................................................................................................................70
17.2 Storage capacity ...............................................................................................................................71
17.3 Setting the storage rate for the “DL1” recorder data.........................................................................71
17.4 Deleting the data recorder.................................................................................................................71
18. USB MENU: DATA TRANSFER VIA USB INTERFACE .....................................72
18.1 Connecting the USB stick .................................................................................................................72
18.2 Import function ..................................................................................................................................73
18.3 Export functions ................................................................................................................................73
18.4 Ongoing data transfer .......................................................................................................................74
18.5 Error during data transmission..........................................................................................................74
18.6 Removing the USB stick ...................................................................................................................74
19. REFERENCE MEASUREMENTS ........................................................................75
19.1 CO2reference measuring .................................................................................................................75
19.1.1 Measuring the CO2concentration indirectly via the pH of the cell medium............................75
19.1.2 Measuring CO2directly via chemical indicator tubes..............................................................76
19.1.3 Measuring CO2directly with an electronic infrared measuring device ...................................77
19.2 Temperature reference measurement..............................................................................................77
20. OPTIONS..............................................................................................................77
20.1 Silicone access ports 30 mm / 1.18 in, closable with 2 silicone plugs (8012-0558 rear, 8012-0559
left, 8012-0560 right) (option)............................................................................................................77
20.2 Base on castors (option)...................................................................................................................78
20.3 Flat stacking frame (option)...............................................................................................................78
20.4 APT-COM™ 4 Multi Management Software (option)........................................................................78
20.5 Data logger kit (option)......................................................................................................................78
20.6 Analog outputs for temperature and CO2(option) ............................................................................79
21. AVOIDING MICROBIAL CONTAMINATION........................................................79
21.1 Cells and media ................................................................................................................................79
21.2 Laboratory conditions / equipment around the CO2incubator..........................................................79
21.3 Working and behavior in the lab .......................................................................................................80
21.4 Chamber design and equipment.......................................................................................................80
21.5 Handling the CO2incubator ..............................................................................................................81
22. CLEANING, DECONTAMINATION / DISINFECTION, AND STERILIZATION....82
22.1 Cleaning............................................................................................................................................83
22.2 Decontamination / chemical disinfection of the chamber..................................................................84
22.3 Disinfection of the CO2sensor..........................................................................................................85

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23. HOT-AIR STERILIZATION AT 180 °C / 356 °F....................................................86
23.1 Overview ...........................................................................................................................................86
23.2 Performing a hot-air sterilization .......................................................................................................87
23.2.1 Preparations for hot-air sterilization........................................................................................87
23.3 Starting and running the hot-air sterilization cycle............................................................................88
23.3.1 Starting sterilization.................................................................................................................88
23.3.2 Performance during sterilization.............................................................................................89
23.3.3 Completing the entire sterilization cycle .................................................................................89
23.4 Prematurely terminating the sterilization cycle – effects...................................................................90
23.4.1 Premature termination after less than 6 hours: Ineffective sterilization..................................90
23.4.2 Premature termination after more than 6 hours, i.e., during the cooling-down phase:
successful sterilization............................................................................................................91
23.5 Prematurely terminating the sterilization cycle – procedure.............................................................91
23.5.1 Cancelling sterilization via the controller menu ......................................................................91
23.5.2 Opening the outer door...........................................................................................................92
23.5.3 Turning off the chamber..........................................................................................................92
24. MAINTENANCE, AND SERVICE, TROUBLESHOOTING, REPAIR, TESTING..93
24.1 General information, personnel qualification.....................................................................................93
24.2 Maintenance intervals, service..........................................................................................................94
24.3 Service Reminder..............................................................................................................................94
24.4 Gas inlet fine filter .............................................................................................................................95
24.5 Simple troubleshooting......................................................................................................................95
24.5.1 General ...................................................................................................................................95
24.5.2 Temperature ...........................................................................................................................96
24.5.3 CO2.........................................................................................................................................97
24.5.4 Humidity..................................................................................................................................98
24.5.5 Controller ................................................................................................................................98
24.5.6 Sterilization .............................................................................................................................98
24.6 Sending the chamber back to BINDER GmbH.................................................................................99
25. DISPOSAL..........................................................................................................100
25.1 Disposal of the transport packing....................................................................................................100
25.1.1 Outer chamber packing.........................................................................................................100
25.1.2 Packing inside the chamber, equipment...............................................................................100
25.2 Decommissioning............................................................................................................................101
25.3 Disposal of the chamber in the Federal Republic of Germany.......................................................101
25.4 Disposal of the chamber in the member states of the EU except for the Federal Republic of
Germany..........................................................................................................................................102
25.5 Disposal of the chamber in non-member states of the EU .............................................................103
26. TECHNICAL DESCRIPTION..............................................................................104
26.1 Factory calibration and adjustment.................................................................................................104
26.2 Over current protection ...................................................................................................................104
26.3 Definition of usable volume.............................................................................................................104
26.4 CB-S / CB-S-UL technical data.......................................................................................................105
26.5 Important conversion data for non-SI units.....................................................................................106
26.6 Conversion table for gas inlet pressures, bar – psi.........................................................................107
26.7 Equipment and options (extract).....................................................................................................107
26.8 Options, accessories, and spare parts (extract) .............................................................................108
26.9 Dimensions......................................................................................................................................109
27. CERTIFICATES AND DECLARATIONS OF CONFORMITY.............................111
27.1 EU Declaration of Conformity..........................................................................................................111
28. PRODUCT REGISTRATION..............................................................................113
29. CONTAMINATION CLEARANCE CERTIFICATE .............................................114
29.1 For chambers located outside the USA and Canada .....................................................................114
29.2 For chambers in the USA and Canada...........................................................................................117

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Dear Customer,
For the correct operation of the CO2incubator CB-S / CB-S-UL, it is important that you read this operating
manual completely and carefully and observe all instructions as indicated. Failure to read, understand
and follow the instructions may result in personal injury. It can also lead to damage to the chamber and/or
poor equipment performance.
1. Safety
1.1 Personnel Qualification
The chamber must only be installed, tested, and started up by personnel qualified for assembly, startup,
and operation of the chamber. Qualified personnel are persons whose professional education,
knowledge, experience and knowledge of relevant standards allow them to assess, carry out, and identify
any potential hazards in the work assigned to them. They must have been trained and instructed, and be
authorized, to work on the chamber.
The chamber should only be operated by laboratory personnel especially trained for this purpose and
familiar with all precautionary measures required for working in a laboratory. Observe the national regula-
tions on minimum age of laboratory personnel.
1.2 Operating manual
This operating manual is part of the components of delivery. Always keep it handy for reference in the
vicinity of the chamber. If selling the unit, hand over the operating manual to the purchaser.
To avoid injuries and damage observe the safety instructions of the operating manual. Failure to follow
instructions and safety precautions can lead to significant risks.
DANGER
Dangers due to failure to observe the instructions and safety precautions.
Serious injuries and chamber damage. Risk of death.
Observe the safety instructions in this Operating Manual.
Follow the operating procedures in this Operating Manual.

Carefully read the complete operating instructions of the chamber prior to installing and
using the chamber.
Keep the operating manual for future reference
Make sure that all persons who use the chamber and its associated work equipment have
read and understood the Operating Manual.
This Operating Manual is supplemented and updated as needed. Always use the most recent version of
the Operating Manual. When in doubt, call the BINDER Service Hotline for information on the up-to-
dateness and validity of this Operating Manual.
1.3 Legal considerations
This operating manual is for informational purposes only. It contains information for correct and safe in-
stalling, start-up, operation, decommissioning, cleaning and maintenance of the product. Note: the con-
tents and the product described are subject to change without notice.
Understanding and observing the instructions in this operating manual are prerequisites for hazard-free
use and safety during operation and maintenance. Images are to provide basic understanding. They may
deviate from the actual version of the chamber. The actual scope of delivery can, due to optional or spe-
cial design, or due to recent technical changes, deviate from the information and illustrations in these
instructions this operating manual.

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In no event shall BINDER be held liable for any damages, direct or incidental arising out of or related to
the use of this manual.
This operating manual cannot cover all conceivable applications. If you would like additional information,
or if special problems arise that are not sufficiently addressed in this manual, please ask your dealer or
contact us directly, e.g. by phone at the number located on page one of this manual
Furthermore, we emphasize that the contents of this operating manual are not part of an earlier or exist-
ing agreement, description, or legal relationship, nor do they modify such a relationship. All obligations on
the part of BINDER derive from the respective purchase contract, which also contains the entire and ex-
clusively valid statement of warranty administration and the general terms and conditions, as well as the
legal regulations valid at the time the contract is concluded. The statements in this manual neither aug-
ment nor restrict the contractual warranty provisions.
1.4 Structure of the safety instructions
In this operating manual, the following safety definitions and symbols indicate dangerous situations in
accordance with the standards ISO 3864-2 and ANSI Z535.6.
1.4.1 Signal word panel
Depending on the seriousness and probability of serious consequences, potential dangers are identified
with a signal word, the corresponding safety color, and if appropriate, the safety alert symbol.
DANGER
Indicates an imminently hazardous situation that, if not avoided, will result in death or serious
(irreversible) injury.
WARNING
Indicates a potentially hazardous situation which, if not avoided, could result in death or serious
(irreversible) injury
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor
(reversible) injury
NOTICE
Indicates a potentially hazardous situation which, if not avoided, may result in damage to the product
and/or its functions or of a property in its proximity.
1.4.2 Safety alert symbol
Use of the safety alert symbol indicates a risk of injury.
Observe all measures that are marked with the safety alert symbol in order to avoid death or
injury.

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1.4.3 Pictograms
Warning signs
Electrical hazard
Hot surface
Explosive Atmosphere
Stability hazard
Lifting hazard
Gas cylinders
CO2suffocation and
poisoning hazard
Pollution Hazard
Harmful substances
Biohazard
Risk of corrosion and /
or chemical burns
Mandatory action signs
Mandatory regulation
Read operating
instructions
Disconnect the power
plug
Lift with several persons
Environment protection
Wear protective gloves
Wear safety goggles
Prohibition signs
Do NOT touch Do NOT spray with
water
Do NOT climb
Information to be observed in order to ensure optimum function of the product.
1.4.4 Word message panel structure
Type / cause of hazard.
Possible consequences.
∅Instruction on how to avoid the hazard: prohibition
Instruction on how to avoid the hazard: mandatory action
Observe all other notes and information not necessarily emphasized in the same way, in order to avoid
disruptions that could result in direct or indirect injury or property damage.

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1.5 Localization / position of safety labels at the chamber
The following labels are located on the chamber:
Pictograms (Warning signs)
Service label
Hot surface
Risk of injury (UL chambers only).
Observe the safety instructions in the
operating manual.
Figure 1: Position of labels
on the CO2incubator CB-S
Figure 2: Position of labels
on the CO2incubator CB-S-UL
Keep safety labels complete and legible.
Replace safety labels that are no longer legible. Contact BINDER Service for these replacements.

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1.6 Type plate
Position of type plate: left chamber side (seen from front), at the bottom in the middle.
Figure 3: Position of type plate
Figure 4: Type plate (example CB-S 170 standard chamber, 9040-0189)
Indications of the type plate
(example)
Information
BINDER
Manufacturer: BINDER GmbH
CB-S 170
Model designation
CO2 Incubator
Device name: CO2Incubator
Serial No.
000000000000
Serial No. of the chamber
Built
2020
Year of construction
Nominal temperature
190 °C
374 °F
Nominal temperature
IP protection
20
Type of IP protection acc. to standard EN 60529
Temp. safety device
DIN 12880
Temperature safety device acc. to standard DIN 12880:2007
Class
3.1
Class of temperature safety device
Art. No.
9040-0189
Art. No. of the chamber
Project No.
---
Optional: Special application acc. to project no.
1,30 kW
Nominal power
6,1 A
Nominal current
200-230 V / 50 Hz
Nominal voltage range +/- 10%
at the indicated power frequency
200-230 V / 60 Hz
1 N ~
Current type
Nominal temp.
190 °C
1,30 kW / 6,1 A
374 °F
200-230 V / 50 Hz
IP protection
20
200-230 V / 60 Hz
Safety device
DIN 12880
1 N ~
Class
3.1
Art. No.
9040-0189
Project No.
Built
2020
CO2 Incubator
BINDER GmbH
Im Mittleren Ösch 5
78532 Tuttlingen / Germany
www.binder-world.com
CB-S 170
E7
Serial No. 00000000000000
Made in Germany