Binder KB 53 (E4) User manual

Issue 02/2017 Art. No. 7001-0307
Operating Manual
Translation of the original operating manual
KB (E4), KB (E6)
Cooling Incubators
with compressor technology and program control
Model Model version Art. No.
KB 53 (E4) KB053-230V 9020-0199, 9120-0199
KB 53-UL (E4) KB053UL-120V 9020-0302, 9120-0302
KB 115 (E4) KB115-230V 9020-0397, 9120-0397
KB 115-UL (E4) KB115UL-120V 9020-0398, 9120-0398
KB 240 (E6) KB240-230V 9020-0202, 9120-0202
KB 240-UL (E6) KB240UL-120V 9020-0304, 9120-0304
KB 400 (E6) KB400-230V 9020-0203, 9120-0203
KB 400-UL (E6) KB400UL-120V 9020-0305, 9120-0305
KB 720 (E6) KB720-230V 9020-0204, 9120-0204
KB 720-UL (E6) KB720UL-240V 9020-0306, 9120-0306
BINDER GmbH
Address Post office box 102
78502 Tuttlingen, Germany
Tel. [phone]
Fax [phone]
Internet http://www.binder-world.com
E-mail [e-mail]
Service Hotline [phone]
Service Fax [phone]
Service E-Mail [e-mail]
Service Hotline USA [phone] or [phone] x3
Service Hotline Asia Pacific [phone] or [phone]
Service Hotline Russia and CIS [phone]

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Contents
1. SAFETY..................................................................................................................6
1.1 Legal considerations...........................................................................................................................6
1.2 Structure of the safety instructions......................................................................................................6
1.2.1 Signal word panel .....................................................................................................................6
1.2.2 Safety alert symbol ...................................................................................................................7
1.2.3 Pictograms................................................................................................................................7
1.2.4 Word message panel structure.................................................................................................8
1.3 Localization / position of safety labels on the chamber ......................................................................8
1.4 Type plate............................................................................................................................................9
1.5 General safety instructions on installing and operating the chambers .............................................10
1.6 Intended use .....................................................................................................................................12
1.7 Operating instructions .......................................................................................................................12
1.8 Measures to prevent accidents.........................................................................................................13
2. CHAMBER DESCRIPTION..................................................................................14
2.1 Chamber overview............................................................................................................................15
2.2 Instrument panel ...............................................................................................................................16
2.3 Lateral control panel with main power switch and optional equipment –KB 240 / 400 / 720 (E6)..17
2.4 Chamber rear – KB 53 / 115 (E4) .....................................................................................................18
3. COMPLETENESS OF DELIVERY, TRANSPORTATION, STORAGE, AND
INSTALLATION....................................................................................................19
3.1 Unpacking, and checking equipment and completeness of delivery................................................19
3.2 Guidelines for safe lifting and transportation.....................................................................................20
3.3 Storage..............................................................................................................................................20
3.4 Location of installation and ambient conditions ................................................................................21
4. INSTALLATION OF THE EQUIPMENT...............................................................22
4.1 Spacers for wall distance – KB 240 / 400 / 720 (E6) ........................................................................22
4.2 Mounting the flexible tilt protection kit – KB 400 (E6) .......................................................................23
4.3 Electrical connection .........................................................................................................................24
5. START UP............................................................................................................25
6. FUNCTIONAL OVERVIEW OF THE T4.12 CHAMBER CONTROLLER.............25
6.1 Menu structure ..................................................................................................................................26
6.1.1 General menu.........................................................................................................................26
6.1.2 Quick menu.............................................................................................................................28
6.1.3 “User” menu............................................................................................................................28
6.2 Operating modes...............................................................................................................................29
6.2.1 Activating the “control off”mode or change to “fixed value” operating mode.........................29
6.3 Performance during and after power failure......................................................................................31
6.4 Information ........................................................................................................................................32
7. CONFIGURATION OF OPTIONAL EQUIPMENT................................................33
7.1 Turning on / off the optional interior socket.......................................................................................33
7.2 Switching on or off the optional zero-voltage relay control outputs ..................................................34
7.3 Functional test of the optional alarm output......................................................................................34
7.4 Switching on or off the optional object temperature display .............................................................35
7.5 RS 422 address (with optional RS 422 interface).............................................................................35
8. SET-POINT ENTRY IN “FIXED VALUE” OPERATING MODE...........................36
8.1 Setting ranges:..................................................................................................................................36
8.2 Entering the set-points via “quick menu”...........................................................................................36
8.3 Entering the set-points via general menu .........................................................................................38

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9. TIME PROGRAMS ...............................................................................................40
9.1 Starting and running an existing time program.................................................................................42
9.2 Cancelling a running time program...................................................................................................45
9.3 Creating a new time program............................................................................................................46
9.3.1 Section handling .....................................................................................................................48
9.3.2 Temperature setpoint..............................................................................................................49
9.3.3 Section duration......................................................................................................................49
9.3.4 Repeating one or several sections within a time program......................................................50
9.3.5 Tolerance range......................................................................................................................51
9.3.6 Set-point ramp and set-point step modes...............................................................................53
9.3.7 Switching on or off the optional zero-voltage relay outputs....................................................55
9.3.8 Calling up the next parameter.................................................................................................56
9.3.9 Saving the time program and leaving the program editor.......................................................58
9.4 Program interruption .........................................................................................................................60
9.5 Deleting a time program....................................................................................................................61
10. WEEK PROGRAMS.............................................................................................62
10.1 Starting and running an existing week program................................................................................63
10.2 Cancelling a running week program .................................................................................................66
10.3 Creating a new week program..........................................................................................................67
10.3.1 Section handling .....................................................................................................................69
10.3.2 Temperature setpoint..............................................................................................................70
10.3.3 Day of the week......................................................................................................................70
10.3.4 Time of the day.......................................................................................................................71
10.3.5 Activity of the shift-point..........................................................................................................72
10.3.6 Switching on or off the optional zero-voltage relay outputs....................................................72
10.3.7 Calling up the next parameter.................................................................................................73
10.3.8 Saving the week program and leaving the program editor.....................................................75
10.4 Deleting a week program..................................................................................................................76
11. KEY LOCK ...........................................................................................................77
11.1 Directly activating the key lock function ............................................................................................78
11.2 Automatic key lock ............................................................................................................................78
11.3 Changing the password for unlocking the key lock...........................................................................80
12. GENERAL CONTROLLER SETTINGS................................................................81
12.1 Setup wizard .....................................................................................................................................82
12.2 Date and time settings ......................................................................................................................82
12.3 Selecting the menu language of the T4.12 controller .......................................................................84
12.4 Setting display brightness .................................................................................................................84
12.5 Changing the temperature unit..........................................................................................................85
12.6 Defining the data recording rate........................................................................................................85
12.7 Factory reset .....................................................................................................................................86
12.8 Network configuration .......................................................................................................................86
12.9 Display of the entire network configuration.......................................................................................90
12.10 Display and entry of the chamber configuration – for service purpose.............................................90
13. DATA TRANSFER VIA USB INTERFACE...........................................................91
13.1 Exporting data to USB drive..............................................................................................................91
13.2 Importing data from USB drive..........................................................................................................92
14. NOTIFICATIONS AND ALARMS.........................................................................93
14.1 Notifications overview .......................................................................................................................93
14.2 Alarms overview................................................................................................................................93
14.3 Alarm status ......................................................................................................................................94
14.4 Confirming a “set” alarm....................................................................................................................95

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14.5 Alarm configuration and overview.....................................................................................................96
14.5.1 List of active alarms................................................................................................................96
14.5.2 History – list of all alarms........................................................................................................97
14.5.3 Activating, deactivating, and testing the alarm buzzer ...........................................................98
14.5.4 Activating / deactivating all alarm functions............................................................................98
15. EVENT LIST.........................................................................................................99
16. GRAPHICAL DISPLAY OF THE MEASURED VALUES...................................100
1.1 Setting the sampling rate ................................................................................................................100
16.1 Defining the display range...............................................................................................................101
16.2 Selecting the parameters................................................................................................................102
17. TEMPERATURE SAFETY DEVICES.................................................................103
17.1 Over temperature protective device (class 1).................................................................................103
17.2 Overtemperature safety controller (temperature safety device class 3.1)......................................103
17.2.1 Safety controller modes........................................................................................................103
17.2.2 Setting the safety controller ..................................................................................................104
17.3 Over- and undertemperature safety controller (temperature safety device class 3.3) (option) ......107
17.3.1 Safety controller modes........................................................................................................107
17.3.2 Setting the Over- and undertemperature safety controller ...................................................108
18. DEFROSTING DURING REFRIGERATING OPERATION ................................111
19. OPTIONS............................................................................................................112
19.1 Communication software APT-COM™ 3 DataControlSystem (option) ..........................................112
19.2 RS RS 422 interface (option)..........................................................................................................112
19.3 Data logger kit (option)....................................................................................................................112
19.4 Object temperature display with flexible Pt 100 temperature sensor (option) ................................113
19.5 Zero-voltage relay alarm output (may be available via BINDER INDIVIDUAL Customized Solutions)113
19.6 Analog output for temperature (option)...........................................................................................115
19.7 Zero-voltage relay control outputs (may be available via BINDER INDIVIDUAL Customized
Solution)..........................................................................................................................................115
19.8 Water protected internal socket (option).........................................................................................116
19.9 Interior lighting.................................................................................................................................116
20. MAINTENANCE, CLEANING, AND SERVICE ..................................................117
20.1 Maintenance intervals, service........................................................................................................117
20.2 Cleaning and decontamination .......................................................................................................118
20.2.1 Cleaning................................................................................................................................118
20.2.2 Decontamination...................................................................................................................119
20.3 Sending the chamber back to BINDER GmbH...............................................................................120
21. DISPOSAL..........................................................................................................121
21.1 Disposal of the transport packing....................................................................................................121
21.2 Decommissioning............................................................................................................................121
21.3 Disposal of the chamber in the Federal Republic of Germany.......................................................121
21.4 Disposal of the chamber in the member states of the EC except for the Federal Republic of
Germany..........................................................................................................................................123
21.5 Disposal of the chamber in non-member states of the EC.............................................................124
22. TROUBLESHOOTING .......................................................................................125
23. TECHNICAL DESCRIPTION..............................................................................127
23.1 Factory calibration and adjustment.................................................................................................127
23.2 Over current protection ...................................................................................................................127
23.3 Definition of usable volume.............................................................................................................128
23.4 Technical data.................................................................................................................................129
23.5 Equipment and Options (extract) ....................................................................................................131
23.6 Spare parts and accessories (extract) ............................................................................................132

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23.7 KB 53 dimensions ...........................................................................................................................133
23.8 KB 115 dimensions .........................................................................................................................134
23.9 KB 240 dimensions .........................................................................................................................135
23.10 KB 400 dimensions .........................................................................................................................136
23.11 KB 720 dimensions .........................................................................................................................137
24. CERTIFICATES AND DECLARATIONS OF CONFORMITY.............................138
24.1 EU Declaration of Conformity..........................................................................................................138
24.2 Certificate for the GS mark of conformity of the “Deutsche Gesetzliche Unfallversicherung e.V.“
(German Social Accident Insurance) DGUV...................................................................................140
25. PRODUCT REGISTRATION..............................................................................142
26. CONTAMINATION CLEARANCE CERTIFICATE .............................................143
26.1 For chambers located outside USA and Canada ...........................................................................143
26.2 For chambers in USA and Canada.................................................................................................146

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Dear customer,
For the correct operation of the chambers, it is important that you read this operating manual completely
and carefully and observe all instructions as indicated. Observe the national regulations on minimum age
of laboratory personnel. Failure to read, understand and follow the instructions may result in personal
injury. It can also lead to damage to the chamber and/or poor equipment performance.
1. Safety
This operating manual is part of the components of delivery. Always keep it handy for reference. The
device should only be operated by laboratory personnel especially trained for this purpose and familiar
with all precautionary measures required for working in a laboratory. To avoid injuries and damage ob-
serve the safety instructions of the operating manual.
WARNING
Failure to observe the safety instructions.
Serious injuries and chamber damage.
Observe the safety instructions in this operating manual.
Carefully read the complete operating instructions of the chambers.
1.1 Legal considerations
This operating manual is for informational purposes only. It contains information for installing, start-up,
operation and maintenance of the product. Note: the contents and the product described are subject to
change without notice.
Understanding and observing the instructions in this operating manual are prerequisites for hazard-free
use and safety during operation and maintenance. In no event shall BINDER be held liable for any dam-
ages, direct or incidental arising out of or related to the use of this manual.
This operating manual cannot cover all conceivable applications. If you would like additional information,
or if special problems arise that are not sufficiently addressed in this manual, please ask your dealer or
contact us directly by phone at the number located on page one of this manual
Furthermore, we emphasize that the contents of this operating manual are not part of an earlier or exist-
ing agreement, description, or legal relationship, nor do they modify such a relationship. All obligations on
the part of BINDER derive from the respective purchase contract, which also contains the entire and ex-
clusively valid statement of warranty administration. The statements in this manual neither augment nor
restrict the contractual warranty provisions.
1.2 Structure of the safety instructions
In this operating manual, the following safety definitions and symbols indicate dangerous situations fol-
lowing the harmonization of ISO 3864-2 and ANSI Z535.6.
1.2.1 Signal word panel
Depending on the probability of serious consequences, potential dangers are identified with a signal
word, the corresponding safety color, and if appropriate, the safety alert symbol.
DANGER
Indicates an imminently hazardous situation that, if not avoided, will result in death or serious (irreversi-
ble) injury.

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WARNING
Indicates a potentially hazardous situation which, if not avoided, could result in death or serious (irre-
versible) injury.
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor (re-
versible) injury.
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in damage to the product
and/or its functions or of a property in its proximity.
1.2.2 Safety alert symbol
Use of the safety alert symbol indicates a risk of injury.
Observe all measures that are marked with the safety alert symbol in order to avoid death or
injury.
1.2.3 Pictograms
Warning signs
Electrical hazard
Hot surface
Explosive atmosphere
Stability hazard
Lifting hazard
Risk of corrosion and /
or chemical burns
Harmful substances
Biohazard
Pollution Hazard
Mandatory action signs
Mandatory regulation
Read operating
instructions
Disconnect the power
plug
Lift with several persons
Lift with mechanical
assistance
Environment protection
Wear protective gloves
Wear safety goggles

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Prohibition signs
Do NOT touch
Do NOT spray with
water
Do NOT climb
Information to be observed in order to ensure optimum function of the product.
1.2.4 Word message panel structure
Type / cause of hazard.
Possible consequences.
∅Instruction how to avoid the hazard: prohibition
Instruction how to avoid the hazard: mandatory action
Observe all other notes and information not necessarily emphasized in the same way, in order to avoid
disruptions that could result in direct or indirect injury or property damage.
1.3 Localization / position of safety labels on the chamber
The following labels are located on the chamber:
Pictograms (Warning signs)
Hot surface
•Inner glass door above the glass door
handle
Read operating manual
•UL chambers: outer chamber door
•KB with optional interior socket: below
the interior socket
Service label
Figure 1: Position of labels on the chamber (example KB 240-UL)
Keep safety labels complete and legible.
Replace safety labels that are no longer legible. Contact BINDER Service for these replacements.

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1.4 Type plate
The type plate sticks to the left side of the chamber, bottom right-hand.
Figure 2: Type plate (example of KB 240 regular chamber)
Figure 3: Type plate (example of KB 240 optional chamber)
Indications of the type plate (example)
Information
BINDER
Manufacturer: BINDER GmbH
KB 240
Model designation
Cooling incubator
Device name
Serial No.
00000000000000
Serial no. of the chamber
Built
2016
Year of construction
Nominal tempera-
ture
100 °C
212°F
Nominal temperature
IP protection
20
IP type of protection acc. to standard EN 60529
Temp. safety
device
DIN 12880
Temperature safety device acc. to standard DIN
12880:2007
Class
3.1
Class of temperature safety device
Art. No.
9020-0202
Art. no. of the chamber
Project No.
---
Optional: Special application acc. to project no.
1,20 kW
Nominal power
5,2 A
Nominal current
200-230 V / 50 Hz
Nominal voltage range +/-10%
at the indicated power frequency
200-230 V / 60 Hz
1 N ~
Current type
Max. operating pressure 15 bar
Max. operating pressure in the refrigerating system
R 134A - 0,35 kg
Refrigerant type and max. filling weight
Contains fluorinated greenhouse gases
covered by the Kyoto Protocol
Contains fluorinated greenhouse gases covered by the
Kyoto Protocol
With option internal socket:
Nominal power: 1,70 kW
With option internal socket: increased total nominal power
Nominal temp.
100 °C
1,20 kW / 5,2 A
Max. operating pressure 15 bar
212 °F
200-230 V / 50 Hz
R 134A – 0,35 kg
IP protection
20
200-230 V / 60 Hz
Contains fluorinated greenhouse gases
Safety device
DIN 12880
1 N ~
covered by the Kyoto Protocol
Class
3.1
With option internal socket:
Art. No.
9120-0202
Nominal power: 1,70 kW
Project No.
Built
2016
Cooling incubator
BINDER GmbH
Im Mittleren Ösch 5
78532 Tuttlingen / Germany
www.binder-world.com
KB 240
E6
Serial No. 00000000000000
Made in Germany
Nominal temp.
100 °C
1,20 kW / 5,2 A
Max. operating pressure 15 bar
212 °F
200-230 V / 50 Hz
R 134A – 0,35 kg
IP protection
20
200-230 V / 60 Hz
Contains fluorinated greenhouse gases
Safety device
DIN 12880
1 N ~
covered by the Kyoto Protocol
Class
3.1
Art. No.
9020-0202
Project No.
Built
2016
Cooling incubator
BINDER GmbH
Im Mittleren Ösch 5
78532 Tuttlingen / Germany
www.binder-world.com
KB 240
E6
Serial No. 00000000000000
Made in Germany

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Symbol on the type plate
Information
CE conformity marking
Electrical and electronic equipment manufactured / placed on
the market in the EU after 13 August 2005 and to be disposed of
in separate collection according to Directive 2012/19/EU on
waste electrical and electronic equipment (WEEE).
GS mark of conformity of the “Deutsche Gesetzliche Unfallversi-
cherung e.V. (DGUV), Prüf- und Zertifizierungsstelle Nahrung-
smittel und Verpackung im DGUV Test“ (German Social Acci-
dent Insurance (DGUV), Testing and Certification Body for
Foodstuffs and Packaging Industry in DGUV Test).
The equipment is certified according to Customs Union Tech-
nical Regulation (CU TR) for Russia, Belarus and Kazakhstan
(KB-UL only)
The equipment is certified by Underwriters Laboratories Inc.®
according to standards CAN/CSA-C22.2 No. 61010-1, 2nd Edi-
tion, 2004-07 (Electrical Equipment for Measurement, Control,
and Laboratory Use; Part 1: General Requirements); UL 61010-
1, 2nd Edition, 2005-07-22 (Electrical Equipment for Measure-
ment, Control, and Laboratory Use; Part 1: General Require-
ments); IEC 61010-1:2001, 2nd Edition and IEC 61010-2-10
(Particular Requirements for Laboratory Equipment for the heat-
ing of materials).
1.5 General safety instructions on installing and operating the chambers
With regard to operating the chambers and to the installation location, please observe the DGUV guide-
lines 213-850 on safe working in laboratories (formerly BGI/GUV-I 850-0, BGR/GUV-R 120 or ZH 1/119,
issued by the employers’ liability insurance association) (for Germany).
BINDER GmbH is only responsible for the safety features of the chamber provided skilled electricians or
qualified personnel authorized by BINDER perform all maintenance and repair, and if components relat-
ing to chamber safety are replaced in the event of failure with original spare parts.
To operate the chamber, use only original BINDER accessories or accessories from third-party suppliers
authorized by BINDER. The user is responsible for any risk caused by using unauthorized accessories.
CAUTION
Danger of overheating.
Damage to the chamber.
∅Do NOT install the chamber in unventilated recesses.
Ensure sufficient ventilation for dispersal of the heat.
Do not operate the chambers in hazardous locations.
DANGER
Explosion hazard.
Danger of death.
∅Do NOT operate the chamber in potentially explosive areas.
KEEP explosive dust or air-solvent mixtures AWAY from the chamber.

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The chambers do not dispose of any measures of explosion protection.
DANGER
Explosion hazard.
Danger of death.
∅Do NOT introduce any substance into the cooling incubator which is combustible or
explosive at working temperature.
∅NO explosive dust or air-solvent mixture in the inner chamber.
Any solvent contained in the charging material must not be explosive or inflammable. I.e., irrespective of
the solvent concentration in the steam room, NO explosive mixture with air must form. The temperature
inside the chamber must lie below the flash point or below the sublimation point of the charging material.
Familiarize yourself with the physical and chemical properties of the charging material, as well as the
contained moisture constituent and its behavior with the addition of heat energy.
Familiarize yourself with any potential health risks caused by the charging material, the contained mois-
ture constituent or by reaction products which may arise during the temperature process. Take adequate
measures to exclude such risks prior to putting the cooling incubator into operation.
DANGER
Electrical hazard.
Danger of death.
∅The chamber must NOT become wet during operation or maintenance.
The cooling incubators were produced in accordance with VDE regulations and were routinely tested in
accordance to VDE 0411-1 (IEC 61010-1).
During and shortly after operation, the temperature of the inner surfaces almost equals the set-point.
CAUTION
The glass doors, the glass door handles, and the inner chamber will become hot
during operation.
Danger of burning.
∅Do NOT touch the glass doors, the glass door handles, the inner surfaces or the charg-
ing material during operation.
WARNING
Stability hazard.
Danger of injury.
Damage to the chamber and the charging material.
Housing cover breakaway.
∅Do NOT climb on the lower housing cover.
∅Do NOT load the lower housing cover with heavy objects while the chamber door is
open.

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1.6 Intended use
Cooling incubators KB are suitable for exact conditioning of harmless materials. Because of their precise
temperature accuracy these devices are especially useful for cultivation of microorganisms with a narrow
temperature optimum in a range of 4 °C / 39.2°F to 37 °C / 98.6°F. Main fields of application are tests of
long-term storage (e.g. at 4 °C / 39.2°F), refrigerated incubation between 20 °C / 68°F and 25 °C / 77°F
and incubation at 37 °C / 98.6°F (also with additional introduction of heat) or with alternating temperatures
(e.g. 37 °C / 98.6°F and 4 °C / 39.2°F).
A mixture of any component of the charging material with air must NOT be explosive. The operating tem-
perature must lie below the flash point or below the sublimation point of the charging material. Any com-
ponent of the charging material must NOT be able to release toxic gases.
Other applications are not approved.
The chambers are not classified as medical devices as defined by the Medical Device Directive
93/42/EEC.
Following the instructions in this operating manual and conducting regular maintenance work
(chap. 20.1) are part of the intended use.
DANGER
Explosion or implosion hazard.
Danger of poisoning.
Danger of death.
∅Do NOT introduce any substance combustible or explosive at working temperature into
the chamber, in particular no energy sources such as batteries or lithium-ion batteries.
∅NO explosive dust or air-solvent mixture in the inner chamber.
∅Do NOT introduce any substance which could lead to release of toxic gases.
The charging material shall not contain any corrosive ingredients that may damage the ma-
chine components made of stainless steel, aluminum, and copper. Such ingredients include
in particular acids and halides. Any corrosive damage caused by such ingredients is exclud-
ed from liability by BINDER GmbH.
WARNING: If customer should use a BINDER chamber running in non-supervised continu-
ous operation, we strongly recommend in case of inclusion of irrecoverable specimen or
samples to split such specimen or samples and store them in at least two chambers, if this is
feasible.
In case of foreseeable use of the chamber there is no risk for the user through the integration of the
chamber into systems or by special environmental or operating conditions in the sense of EN 61010-
1:2010. For this, the intended use of the chamber and all its connections must be observed.
1.7 Operating instructions
Depending on the application and location of the chamber, the operator of the chamber must provide the
relevant information for safe operation of the chamber in a set of operating instructions.
Keep these operating instructions with the chamber at all times in a place where they are
clearly visible. They must be comprehensible and written in the language of the employees.

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1.8 Measures to prevent accidents
The operator of the chamber must observe the following rule: “Betreiben von Arbeitsmitteln. Betreiben
von Kälteanlagen, Wärmepumpen und Kühleinrichtungen“ (Operation of work equipment. Operation of
refrigeration systems, heat pumps and refrigeration equipment) (GUV-R 500 chap. 2.35) (for Germany).
The manufacturer took the following measures to prevent ignition and explosions:
•Indications on the type plate
See operating manual chap. 1.4.
•Operating manual
An operating manual is available for each chamber.
•Overtemperature monitoring
The chamber is equipped with a temperature display, which can be read from outside.
The chamber is equipped with an additional safety controller (temperature safety device class 3.1 acc.
to DIN 12880:2007). Visual and audible (buzzer) signals indicate temperature exceeding.
•Safety, measurement, and control equipment
The safety, measuring, and control equipment is easily accessible.
•Electrostatic charge
The interior parts are grounded.
•Non-ionizing radiation
Non-ionizing radiation is not intentionally produced, but released only for technical reasons by electri-
cal equipment (e.g. electric motors, power cables, solenoids). The machine has no permanent mag-
nets. If persons with active implants (e.g. pacemakers, defibrillators) keep a safe distance (distance of
field source to implant) of 30 cm, an influence of these implants can be excluded with high probability.
•Protection against touchable surfaces
Tested according to EN ISO 13732-1:2008.
•Floors
See operating manual chap. 3.4 for correct installation
•Cleaning
See operating manual chap. 20.2.
•Examinations
The chamber has been inspected by the “Deutsche Gesetzliche Unfallversicherung e.V. (DGUV)
(German Social Accident Insurance (DGUV)” (German Social Accident Insurance (DGUV), Testing
and Certification Body for Foodstuffs and Packaging Industry in DGUV Test) and bears the GS mark.

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2. Chamber description
A high level of precision, reliability, and safety for all growth parameters ensures optimum incubation con-
ditions. Moreover, the KB cooling incubator is designed for maximum usability – even in continuous oper-
ation year after year. It fulfills all technical and application-specific requirements arising in experimentation
such as in the areas of biotechnology, medicine, the nutrition industry, pharmaceutical and cosmetics
industries, botany, and zoology.
Two important temperature technologies have been combined to achieve perfect temperature control.
The specially developed DCT™ refrigerating system, a direct refrigerating process, in conjunction with the
APT.line™ preheating chamber technology, satisfies the unique prerequisites for attaining highly-precise
temperature control and particularly short recovery times after opening the door.
The refrigerating system is distinguished by direct, precise, and rapid temperature conduction. Large-area
labyrinth evaporator plates directly conduct the cold to the atmosphere of the working space.
The APT.line™ preheating chamber system ensures high level of spatial and time-based temperature
precision, thanks to the direct and distributed air circulation into the interior. This is especially important
for maintaining temperatures – especially with full chambers – and for rapid restoration of optimum growth
conditions after opening the door. The inner glass door ensures that the temperature remains constant
when observing the incubation process. The fan supports exact attainment and maintenance of the de-
sired temperature accuracy. The fan speed is digitally adjustable. The heating and refrigerating systems
are microprocessor regulated to a tenth of a degree. In addition, the chamber provides almost unlimited
possibilities for adaptation to individual customer requirements based upon extensive programming op-
tions and on the week program timer and real time clock of the controller.
The KB 240 / 400 / 720 (E6) chambers are equipped with a door heating.
All chamber functions are easy and comfortable to use thanks to their clear arrangement. Major features
are easy cleaning of all chamber parts and avoidance of undesired contamination.
The inner chamber, the pre-heating chamber and the interior side of the doors are all made of stainless
steel V2A (German material no. 1.4301, US equivalent AISI 304). The housing is RAL 7035 powder-
coated. All corners and edges are also completely coated.
The chamber comes equipped with an Ethernet interface for computer communication, e.g. via the com-
munication software APT-COM™ 3 DataControlSystem (option, chap. 19.1). For further options, see
chap. 23.5.
The KB 240 / 400 / 720 (E6) chambers are equipped with four castors. Both front castors can be easily
locked via the attached brakes.
Temperature range at ambient temperature of 25 °C / 77 °F: -5 °C / 23 °F up to +100 °C / 212 °F

KB (E4 + E6) 02/2017 page 15/148
2.1 Chamber overview
Instrument panel with microprocessor
controller T4.12 and USB interface
Door handle
Chamber door
Refrigerating module
Main power switch (1)
Figure 4: Cooling incubator KB 53 / 115 (E4)
Instrument panel with microprocessor
controller T4.12 and USB interface
Door handle
Chamber door
Refrigerating module
Lateral control panel with main power
switch and connections
Figure 5: Cooling incubator KB 240 / 400 / 720 (E6)

KB (E4 + E6) 02/2017 page 16/148
2.2 Instrument panel
5,7" controller display
Context-sensitive buttons
USB interface
Operating button
Pilot lamp: ready for operation
Figure 6: Instrument panel with microprocessor controller T4.12 and USB interface

KB (E4 + E6) 02/2017 page 17/148
2.3 Lateral control panel with main power switch and optional equipment –
KB 240 / 400 / 720 (E6)
(1)
(2)
(3)
(4)
(5)
(6)
(7)
Figure 7: Lateral control panel at the right side of the refrigerating machine, with options
(1) Main power switch
(2) DIN-socket for additional Pt 100 temperature sensor (may be available via BINDER INDIVIDUAL
Customized Solutions)
(3) DIN socket for analog output 4-20 mA (option)
(4) RS422 interface (option)
(5) DIN-socket for zero-voltage relay control outputs (may be available via BINDER INDIVIDUAL Cus-
tomized Solutions)
(6) DIN-socket for zero-voltage relay alarm output (option)
(7) Ethernet interface for computer communication

KB (E4 + E6) 02/2017 page 18/148
2.4 Chamber rear – KB 53 / 115 (E4)
(6)
(2)
(3)
(5)
(7)
Figure 8: Chamber rear with position of options (example)
(2) DIN-socket for additional Pt 100 temperature sensor (may be available via BINDER INDIVIDUAL
Customized Solutions)
(3) DIN socket for analog output 4-20 mA (option)
(4) RS422 interface (option)
(5) DIN-socket for zero-voltage relay control outputs (may be available via BINDER INDIVIDUAL Cus-
tomized Solutions)
(6) DIN-socket for zero-voltage relay alarm output (option)
(7) Ethernet interface for computer communication
(8) Power cable
4)
(8)(

KB (E4 + E6) 02/2017 page 19/148
3. Completeness of delivery, transportation, storage, and installa-
tion
3.1 Unpacking, and checking equipment and completeness of delivery
After unpacking, please check the chamber and its optional accessories, if any, based on the delivery
receipt for completeness and for transportation damage. Inform the carrier immediately if transportation
damage has occurred.
The final tests of the manufacturer may have caused traces of the shelves on the inner surfaces. This has
no impact on the function and performance of the chamber.
Please remove any transportation protection devices and adhesives in/on the chamber and on the doors
and remove the operating manuals and accessory equipment.
CAUTION
Sliding or tilting of the chamber.
Damage to the chamber.
Risk of injury by lifting heavy loads.
Do NOT lift or transport the chamber using the door, the handle, or the lower housing.
Lift KB 53 / 115 from the pallet with the aid of four people in the area of all 4 chamber
feet.
Lift KB 240 from the pallet with the aid of six people in the area of all 4 chamber feet or
use a fork lifter. Set the fork lifter only from the front or rear in the middle of the cham-
ber.
Do NOT lift KB 400 / 720 by hand.
Lift KB 400 / 720 using technical devices (fork lifter) from the pallet. Set the fork lifter
only from the front or rear in the middle of the chamber.
Do NOT set the fork lifter from the chamber side.
If you need to return the chamber, please use the original packing and observe the guidelines for safe
lifting and transportation (chap. 3.2).
For disposal of the transport packing, see chap. 21.1.
Note on second-hand chambers (Ex-Demo-Units):
Second-hand chambers are chambers that were used for a short time for tests or exhibitions. They are
thoroughly tested before resale. BINDER ensures that the chamber is technically sound and will work
flawlessly.
Second-hand chambers are marked with a sticker on the chamber door. Please remove the sticker before
commissioning the chamber.

KB (E4 + E6) 02/2017 page 20/148
3.2 Guidelines for safe lifting and transportation
The front castors of KB 240 / 400 / 720 can be blocked by brakes. Please move the chambers with cas-
tors only when empty and on an even surface, otherwise the castors may be damaged. After operation,
please observe the guidelines for temporarily decommissioning the chamber (chap. 21.2).
CAUTION
Sliding or tilting of the chamber.
Damage to the chamber.
Risk of injury by lifting heavy loads.
Transport the chamber in its original packaging only.
For moving or shipping, secure the chamber with transport straps.
Do NOT lift or transport the chamber using the door, the handle, or the lower housing.
Lift KB 53 / 115 with the aid of four people in the area of all 4 chamber feet.
Lift KB 240 with the aid of 6 people or with a fork lifter. Set the fork lifter only from the
front or rear in the middle of the chamber.
Do NOT lift KB 400 / 720 by hand.
Lift KB 400 / 720 using technical devices (fork lifter). Set the fork lifter only from the
front or rear in the middle of the chamber.
Do NOT set the fork lifter from the chamber side.
•Permissible ambient temperature range during transport: -10 °C / 14°F to +60 °C / 140°F.
You can order transport packing for moving or shipping purposes from BINDER service.
3.3 Storage
Intermediate storage of the chamber is possible in a closed and dry room. Observe the guidelines for
temporary decommissioning (chap. 21.2).
•Permissible ambient temperature range during storage: -10 °C / 14°F to +60 °C / 140°F.
•Permissible ambient humidity: max. 70 % r.H., non-condensing
When after storage in a cold location you transfer the chamber to its warmer installation site, condensa-
tion may form. Before start-up, wait at least one hour until the chamber has attained ambient temperature
and is completely dry.