Binder APT.line KT 53 User manual

Issue 09/2013 Art. No. 7001-0241
Operating Manual
APT.line™ KT (E6)
Refrigerated Incubator
with microprocessor program controller T4.12
and Peltier Refrigerating Technology
Model
Art. No.
KT 53 (E6)
9020-0250, 9120-0250
KT 53-UL (E6)
9020-0255, 9120-0255
KT 115 (E6)
9020-0209, 9120-0209
KT 115-UL (E6)
9020-0227, 9120-0227
BINDER GmbH
Address Post office box 102
D-78502 Tuttlingen
Tel. [phone]
Fax [phone]
Internet http://www.binder-world.com
E-mail [e-mail]
Service Hotline [phone]
Service Fax [phone]
Service E-Mail [e-mail]
Service Hotline USA [phone] or [phone] x3
Service Hotline Asia Pacific [phone] or [phone]
Service Hotline Russia and CIS [phone]

KT (E6) 09/2013 Page 2/135
EG – declaration of conformity
EG – KONFORMITÄTSERKLÄRUNG
EC - DECLARATION OF CONFORMITY
CE - DECLARATION DE CONFORMITE
Anbieter / Supplier / Fournisseur: BINDER GmbH
Anschrift / Address / Adresse:
Im Mittleren Ösch 5, D-78532 Tuttlingen
Produkt / Product / Produit: Kühlbrutschränke mit Programmregelung
Refrigerated incubators with program control
Incubateurs réfrigérés à régulation programmable
Typenbezeichnung / Type / Type:
KT 53, KT 115
Die oben beschriebenen Produkte sind konform mit folgenden EG-Richtlinien:
The products described above are in conformity with the following EC guidelines:
Les produits décrits ci-dessus sont conformes aux directives CE suivantes:
Niederspannungsrichtlinie
2006/95/EG
Low voltage directive
2006/95/EC
Directive basse tension
2006/95/CE
Richtlinie 2006/95/EG des Europäischen Parlaments und des
Rates vom 12. Dezember 2006 zur Angleichung der
Rechtsvorschriften der Mitgliedstaaten betreffend elektrische
Betriebsmittel zur Verwendung innerhalb bestimmter
Spannungsgrenzen
Council Directive 2006/95/EC of 12 December 2006 on the
harmonization of the laws of Member States relating to electrical
equipment designed for use within certain voltage limits
Directive 2006/95/CE du Parlement Européen et du Conseil du 12
décembre 2006 concernant le rapprochement des législations des
États membres relatives au matériel électrique destiné à être
employé dans certaines limites de tension
EMV-Richtlinie
2004/108/EG
EMC Directive
2004/108/EC
Directive CEM
2004/108/CE
Richtlinie 2004/108/EG des Europäischen Parlaments und des
Rates vom 15. Dezember 2004 zur Angleichung der
Rechtsvorschriften der Mitgliedstaaten über die
elektromagnetische Verträglichkeit und zur Aufhebung der
Richtlinie 89/336/EWG.
Directive 2004/108/EC of the European Parliament and of the
Council of 15 December 2004 on the approximation of the laws of
the Member States relating to electromagnetic compatibility and
repealing Directive 98/336/EEC.
Directive 2004/108/CE du Parlement Européen et du Conseil du
15 décembre 2004 relative au rapprochement des législations des
États membres concernant la compatibilité électromagnétique et
abrogeant le directive 98/336/CEE.
Die oben beschriebenen Produkte tragen entsprechend die Kennzeichnung CE.
The products described above, corresponding to this, bear the CE-mark.
Les produits décrits ci-dessus, en correspondance, portent l’indication CE.
1 / 3

KT (E6) 09/2013 Page 3/135
Die oben beschriebenen Produkte sind konform mit folgenden harmonisierten Normen:
The products described above are in conformity with the following harmonized standards:
Les produits décrits ci-dessus sont conformes aux normes harmonisées suivantes:
Sicherheit / safety / sécurité:
EN 61010-1:2010 Sicherheitsbestimmungen für elektrische Mess-, Steuer-, Regel- und
Laborgeräte – Teil 1: Allgemeine Anforderungen (DIN EN 61010-
1:2011, VDE 411-1:2011)
Safety requirements for electrical equipment for measurement, control,
and laboratory use – Part 1: General requirements (IEC 61010-1:2010,
BS EN 61010-1:2010)
Règles de sécurité pour appareils électriques de mesurage, de
régulation et de laboratoire – Partie 1: Prescriptions générales (CEI
61010-1:2010, NF EN 61010:2011)
EN 61010-2-010:2003 Sicherheitsbestimmungen für elektrische Meß-, Steuer-, Regel- und
Laborgeräte – Teil 2-010: Besondere Anforderungen an Laborgeräte für
das Erhitzen von Stoffen (DIN EN 61010-2-010:2004)
Safety requirements for electrical equipment for measurement, control,
and laboratory use – Part 2-010: Particular requirements for laboratory
equipment for the heating of materials (IEC 61010-2-10:2005, BS EN
61010-2-10:2003)
Règles de sécurité pour appareils électriques de mesurage, de
régulation et de laboratoire – Partie 2-010 : Prescriptions particulières
pour appareils de laboratoire utilisés pour l’échauffement des matières
(CEI 61010-2-10:2003, NF EN 61010-2-10:2005)
EMV / EMC / CEM:
EN 61326-1:2006
+ Corr. 1:2008 + Corr. 2:2010
Elektrische Mess-, Steuer-, Regel- und Laborgeräte - EMV-
Anforderungen - Teil 1: Allgemeine Anforderungen (DIN EN 61326-
1:2006 + Berichtigung 1:2008 + Berichtigung 2:2011)
Electrical equipment for measurement, control and laboratory use -
EMC requirements - Part 1: General requirements (IEC 61326-1:2005 +
Corr. 1:2008 + Corr. 2:2010, BS EN 61326-1:2006+ A1:2008)
Matériel électrique de mesure, de commande et de laboratoire -
Exigences relatives à la CEM - Partie 1: Exigences générales (CEI
61326-1:2005 + AC1:2008, NF EN 61326-1:2006 mod.)
EN 61326-2-2:2006 Elektrische Mess-, Steuer-, Regel- und Laborgeräte – EMV-
Anforderungen. Teil 2-2: Besondere Anforderungen - Prüfanordnung,
Betriebsbedingungen und Leistungsmerkmale für ortsveränderliche
Prüf-, Mess- und Überwachungsgeräte in Niederspannungs-
Stromversorgungsnetzen. (DIN EN 61326-2-2:2006)
Electrical equipment for measurement, control and laboratory use –
EMC requirements. Part 2-2: Particular requirements - Test
configurations, operational conditions and performance criteria for
portable test, measuring and monitoring equipment used in low-voltage
distribution systems. (IEC 61326-2-2:2005, BS EN 61326-2-2:2006)
Matériel électrique de mesure, de commande et de laboratoire –
Exigences relatives à la CEM. Partie 2-2: Exigences particulières -
Configurations d’essai, conditions de fonctionnement et critères
d’aptitude à la fonction des matériels portatifs d’essai, de mesure et de
surveillance utilisés dans des systèmes de distribution basse tension.
(CEI 61326-2-2:2005 + AC1:2007, NF EN 61326-2-2:2006)
2 / 3

KT (E6) 09/2013 Page 4/135
D-78532 Tuttlingen, 20.07.2012
BINDER GmbH
P. M. Binder
Geschäftsführender Gesellschafter
Managing Director
Directeur général
B. Hofmann
Leiter F & E
Director R & D
Chef de service R&D
3 / 3

KT (E6) 09/2013 Page 5/135
Product registration

KT (E6) 09/2013 Page 6/135
Contents
EG – declaration of conformity......................................................................................................................2
Product registration .......................................................................................................................................5
1. SAFETY................................................................................................................ 10
1.1 Legal considerations .........................................................................................................................10
1.2 Structure of the safety instructions....................................................................................................10
1.2.1 Signal word panel ...................................................................................................................10
1.2.2 Safety alert symbol .................................................................................................................11
1.2.3 Pictograms..............................................................................................................................11
1.2.4 Word message panel structure...............................................................................................12
1.3 Localization / position of safety labels on the unit.............................................................................12
1.4 Type plate .........................................................................................................................................13
1.5 General safety instructions on installing and operating the refrigerated incubator...........................14
1.6 Intended use .....................................................................................................................................15
2. UNIT DESCRIPTION ............................................................................................ 16
2.1 Unit overview.....................................................................................................................................17
2.2 Instrument panel ...............................................................................................................................17
2.3 Unit rear.............................................................................................................................................18
3. COMPLETENESS OF DELIVERY, TRANSPORTATION, STORAGE, AND
LOCATION OF INSTALLATION.......................................................................... 19
3.1 Unpacking, and checking equipment and completeness of delivery................................................19
3.2 Guidelines for safe lifting and transportation.....................................................................................19
3.3 Storage..............................................................................................................................................20
3.4 Location of installation and ambient conditions ................................................................................20
4. INSTALLATION OF THE EQUIPMENT ............................................................... 21
4.1 Spacer for wall distance....................................................................................................................21
4.2 Electrical connection .........................................................................................................................22
5. START UP............................................................................................................ 23
6. FUNCTIONAL OVERVIEW OF THE T4.12 CHAMBER CONTROLLER ............. 23
6.1 Menu structure ..................................................................................................................................24
6.1.1 General menu .........................................................................................................................24
6.1.2 Quick menu.............................................................................................................................26
6.1.3 “User” menu............................................................................................................................26
6.2 Operating modes...............................................................................................................................27
6.2.1 Activating the “control off”mode or change to “fixed value” operating mode.........................27
6.3 Performance during and after power failure......................................................................................29
6.4 Information ........................................................................................................................................30
7. CONFIGURATION OF OPTIONAL EQUIPMENT................................................ 31
7.1 Setting the optional door heating ......................................................................................................31
7.2 Turning on / off the optional interior socket.......................................................................................32
7.3 Switching on or off the optional zero-voltage relay control outputs ..................................................32
7.4 Functional test of the optional alarm output......................................................................................33
7.5 Switching on or off the optional object temperature display .............................................................33
8. SET-POINT ENTRY IN “FIXED VALUE” OPERATING MODE........................... 34
8.1 Setting ranges: ..................................................................................................................................34
8.2 Entering the set-points via “quick menu”...........................................................................................34
8.3 Entering the set-points via general menu .........................................................................................36

KT (E6) 09/2013 Page 7/135
9. TIME PROGRAMS ............................................................................................... 38
9.1 Starting and running an existing time program .................................................................................40
9.2 Cancelling a running time program...................................................................................................43
9.3 Creating a new time program............................................................................................................44
9.3.1 Section handling .....................................................................................................................46
9.3.2 Temperature setpoint..............................................................................................................47
9.3.3 Section duration......................................................................................................................47
9.3.4 Repeating one or several sections within a time program......................................................48
9.3.5 Tolerance range......................................................................................................................49
9.3.6 Set-point ramp and set-point step modes...............................................................................51
9.3.7 Switching on or off the optional zero-voltage relay outputs....................................................53
9.3.8 Calling up the next parameter.................................................................................................54
9.3.9 Saving the time program and leaving the program editor.......................................................56
9.4 Program interruption .........................................................................................................................58
9.5 Deleting a time program....................................................................................................................59
10. WEEK PROGRAMS ............................................................................................. 60
10.1 Starting and running an existing week program ...............................................................................61
10.2 Cancelling a running week program .................................................................................................64
10.3 Creating a new week program ..........................................................................................................65
10.3.1 Section handling .....................................................................................................................67
10.3.2 Temperature setpoint..............................................................................................................68
10.3.3 Day of the week ......................................................................................................................68
10.3.4 Time of the day .......................................................................................................................69
10.3.5 Activity of the shift-point..........................................................................................................70
10.3.6 Switching on or off the optional zero-voltage relay outputs....................................................70
10.3.7 Calling up the next parameter.................................................................................................71
10.3.8 Saving the week program and leaving the program editor.....................................................73
10.4 Deleting a week program ..................................................................................................................74
11. KEY LOCK ........................................................................................................... 75
11.1 Directly activating the key lock function ............................................................................................76
11.2 Automatic key lock ............................................................................................................................76
11.3 Changing the password for unlocking the key lock...........................................................................78
12. GENERAL CONTROLLER SETTINGS................................................................ 79
12.1 Setup wizard .....................................................................................................................................80
12.2 Date and time settings ......................................................................................................................80
12.3 Selecting the menu language of the T4.12 controller .......................................................................82
12.4 Setting display brightness .................................................................................................................82
12.5 Changing the temperature unit .........................................................................................................83
12.6 Defining the data recording rate........................................................................................................83
12.7 Factory reset .....................................................................................................................................84
12.8 Network configuration .......................................................................................................................84
12.9 Display of the entire network configuration.......................................................................................88
12.10 MAC Address ....................................................................................................................................88
12.11 RS 422 address (with optional RS 422 interface).............................................................................89
12.12 Display and entry of the chamber configuration – for service purpose.............................................89
13. DATA TRANSFER VIA USB INTERFACE .......................................................... 90
13.1 Exporting data to USB drive..............................................................................................................90
13.2 Importing data from USB drive..........................................................................................................91
14. NOTIFICATIONS AND ALARMS ......................................................................... 92
14.1 Notifications overview .......................................................................................................................92
14.2 Alarms overview................................................................................................................................92
14.3 Confirming an active alarm ...............................................................................................................93

KT (E6) 09/2013 Page 8/135
14.4 Alarm configuration and overview.....................................................................................................94
14.4.1 List of active alarms ................................................................................................................95
14.4.2 History – list of all alarms........................................................................................................95
14.4.3 Activating, deactivating, and testing the alarm buzzer ...........................................................96
14.4.4 Activating / deactivating all alarm functions............................................................................97
15. EVENT LIST ......................................................................................................... 98
16. GRAPHICAL DISPLAY OF THE MEASURED VALUES ..................................... 99
16.1 Setting the sampling rate ..................................................................................................................99
16.2 Defining the display range...............................................................................................................100
16.3 Selecting the parameters ................................................................................................................101
17. TEMPERATURE SAFETY DEVICES................................................................. 102
17.1 Overtemperature protective device (class 1) ..................................................................................102
17.2 Overtemperature safety controller (temperature safety device class 3.1) ......................................102
17.2.1 Safety controller modes ........................................................................................................102
17.2.2 Setting the safety controller ..................................................................................................103
17.3 Over- and undertemperature safety controller (temperature safety device class 3.3) (option) ......106
17.3.1 Safety controller modes ........................................................................................................106
17.3.2 Setting the Safety controller..................................................................................................107
18. NOTES ON REFRIGERATING OPERATION .................................................... 110
19. OPTIONS............................................................................................................ 110
19.1 Communication software APT-COM™ 3 DataControlSystem (option)...........................................110
19.2 RS 422 interface (option) ................................................................................................................110
19.3 Data logger kit (option)....................................................................................................................111
19.4 Object temperature display with flexible Pt 100 temperature sensor (option) ................................111
19.5 Zero-voltage relay alarm output (option).........................................................................................112
19.6 Analog output for temperature (option)...........................................................................................113
19.7 Zero-voltage relay control outputs (option) .....................................................................................113
19.8 Water protected internal socket (option).........................................................................................114
20. MAINTENANCE, CLEANING, AND SERVICE .................................................. 115
20.1 Maintenance intervals, service........................................................................................................115
20.2 Cleaning and decontamination .......................................................................................................116
20.2.1 Cleaning................................................................................................................................116
20.2.2 Decontamination...................................................................................................................117
20.3 Sending the unit back to BINDER GmbH .......................................................................................118
21. DISPOSAL ......................................................................................................... 119
21.1 Disposal of the transport packing....................................................................................................119
21.2 Decommissioning............................................................................................................................119
21.3 Disposal of the unit in the Federal Republic of Germany ...............................................................119
21.4 Disposal of the unit in the member states of the EC except for the Federal Republic of Germany120
21.5 Disposal of the unit in non-member states of the EC .....................................................................121
22. TROUBLESHOOTING ....................................................................................... 122
23. TECHNICAL DESCRIPTION.............................................................................. 124
23.1 Factory calibration and adjustment.................................................................................................124
23.2 Over current protection ...................................................................................................................124
23.3 Definition of usable volume.............................................................................................................124
23.4 KT technical data ............................................................................................................................125
23.5 Equipment and Options...................................................................................................................126
23.6 Spare parts and accessories ..........................................................................................................127
23.7 Dimensions KT 53...........................................................................................................................128
23.8 Dimensions KT 115.........................................................................................................................129

KT (E6) 09/2013 Page 9/135
24. CONTAMINATION CLEARANCE CERTIFICATE ............................................. 130
24.1 For units located outside North America and Central America.......................................................130
24.2 For units in North America and Central America ............................................................................133

KT (E6) 09/2013 Page 10/135
Dear customer,
For the correct operation of the refrigerated incubator KT, it is important that you read this operating
manual completely and carefully and observe all instructions as indicated. Failure to read, understand
and follow the instructions may result in personal injury. It can also lead to damage to the unit and/or poor
equipment performance
1. Safety
This operating manual is part of the components of delivery. Always keep it handy for reference. The
device should only be operated by laboratory personnel especially trained for this purpose and familiar
with all precautionary measures required for working in a laboratory. To avoid injuries and damage
observe the safety instructions of the operating manual.
WARNING
Failure to observe the safety instructions.
Serious injuries and unit damage.
Observe the safety instructions in this operating manual.
Carefully read the complete operating instructions of the refrigerated incubator KT.
1.1 Legal considerations
This operating manual is for informational purposes only. It contains information for installing, start-up,
operation and maintenance of the product. Note: the contents and the product described are subject to
change without notice.
Understanding and observing the instructions in this operating manual are prerequisites for hazard-free
use and safety during operation and maintenance. In no event shall BINDER be held liable for any
damages, direct or incidental arising out of or related to the use of this manual.
This operating manual cannot cover all conceivable applications. If you would like additional information,
or if special problems arise that are not sufficiently addressed in this manual, please ask your dealer or
contact us directly by phone at the number located on page one of this manual
Furthermore, we emphasize that the contents of this operating manual are not part of an earlier or
existing agreement, description, or legal relationship, nor do they modify such a relationship. All
obligations on the part of BINDER derive from the respective purchase contract, which also contains the
entire and exclusively valid statement of warranty administration. The statements in this manual neither
augment nor restrict the contractual warranty provisions.
1.2 Structure of the safety instructions
In this operating manual, the following safety definitions and symbols indicate dangerous situations
following the harmonization of ISO 3864-2 and ANSI Z535.6.
1.2.1 Signal word panel
Depending on the probability of serious consequences, potential dangers are identified with a signal
word, the corresponding safety color, and if appropriate, the safety alert symbol.
DANGER
Indicates an imminently hazardous situation that, if not avoided, will result in death or serious
(irreversible) injury.

KT (E6) 09/2013 Page 11/135
WARNING
Indicates a potentially hazardous situation which, if not avoided, could result in death or serious
(irreversible) injury.
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor
(reversible) injury.
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in damage to the product
and/or its functions or of a property in its proximity.
1.2.2 Safety alert symbol
Use of the safety alert symbol indicates a risk of injury.
Observe all measures that are marked with the safety alert symbol in order to avoid death or
injury.
1.2.3 Pictograms
Warning signs
Electrical hazard
Hot surface
Explosive atmosphere
Stability hazard
Lifting hazard
Harmful substances
Risk of corrosion and /
or chemical burns
Biohazard
Pollution Hazard
Mandatory action signs
Mandatory regulation Read operating
instructions
Disconnect the power
plug
Lift with several persons
Environment protection
Wear protective gloves
Wear safety goggles

KT (E6) 09/2013 Page 12/135
Prohibition signs
Do NOT touch
Do NOT spray with
water
Information to be observed in order to ensure optimum function of the product.
1.2.4 Word message panel structure
Type / cause of hazard.
Possible consequences.
Instruction how to avoid the hazard: prohibition
Instruction how to avoid the hazard: mandatory action
Observe all other notes and information not necessarily emphasized in the same way, in order to avoid
disruptions that could result in direct or indirect injury or property damage.
1.3 Localization / position of safety labels on the unit
The following labels are located on the unit:
Pictograms (Warning signs)
Service label
Hot surface
Risk of injury (UL units only)
Unit front KT
Unit front KT-UL
Figure 1: Position of labels on the unit
Keep safety labels complete and legible.
Replace safety labels that are no longer legible. Contact BINDER service for these replacements.

KT (E6) 09/2013 Page 13/135
1.4 Type plate
The type plate is located on the left unit side, on the bottom, right side.
Figure 2: Type plate (example of KT 115 regular unit)
Indications of the type plate
Information
BINDER
Manufacturer: BINDER GmbH
KT 115
Model KT 115
Serial No.
00-00000
Serial No. 00-00000
Nominal temperature
100 °C
212 °F
Nominal temperature
Enclosure protection
IP 20
IP type of protection 20 acc. to EN 60529
Temp. safety device
DIN 12880
Temperature safety device acc. to standard DIN 12880
Class
3.1
Temperature safety device, class 3.1
Art. No.
9020-0209
Art. No. 9020-0209
Project No.
---
(Special application acc. to project no.)
0.70 kW
Nominal power 0.70 kW
200-240 V 1 N ~
Nominal voltage 200-240 V
±
10%, single-phase unit
3.0 A
Nominal current 3.0 Amp
50/60 Hz
Power frequency 50/60 Hz
Thermoelectric cooling Peltier
Peltier refrigerating technology
Symbol on the type plate
Information
CE conformity marking
Electrical and electronic equipment manufactured / placed on
the market in the EC after 13 August 2005 and to be disposed of
in separate collection according to directive 2002/96/EC on
waste electrical and electronic equipment (WEEE).
The equipment is certified in the GOST R certification system of
GOSTSTANDARD Russia.
LABORATORY EQUIPMENT
43KM
(KT-UL only)
The equipment is certified by Underwriters Laboratories Inc.
®
according to standards CAN/CSA-C22.2 No. 61010-1, 2nd
Edition, 2004-07 (Electrical Equipment for Measurement,
Control, and Laboratory Use; Part 1: General Requirements); UL
61010-1, 2nd Edition, 2005-07-22 (Electrical Equipment for
Measurement, Control, and Laboratory Use; Part 1: General
Requirements); IEC 61010-1:2010, 3rd Edition and IEC 61010-2-
10:2003 (Particular Requirements for Laboratory Equipment for
the heating of materials).
Nominal temperature
100°C
0,70 kW
Thermoelectric cooling
212°F
200-240 V 1 N ~
Peltier
Enclosure protection
Temp. safety device
IP 20
DIN 12880
3,0 A
50/60 Hz
Class
3.1
Art. No.
9020-0209
US PATS 4585923 / 5222612 / 5309981
Project No. 5405194 / 5601143 / 5773287 / 6079403
D 78532 Tuttlingen / Germany
Tel. + 49 (0) 7462/ 2005-0
Internet: www.binder-world.com
KT 115 Serial No. 00-00000
Made in Germany

KT (E6) 09/2013 Page 14/135
1.5 General safety instructions on installing and operating the refrigerated
incubator
With regard to operating the refrigerated incubator KT and to the installation location, please observe the
guideline BGI/GUV-I 850-0 on safe working in laboratories (formerly BGR/GUV-R 120 or ZH 1/119
laboratory guidelines issued by the employers’ liability insurance association) (for Germany).
BINDER GmbH is only responsible for the safety features of the unit provided skilled electricians or
qualified personnel authorized by BINDER perform all maintenance and repair, and if components
relating to chamber safety are replaced in the event of failure with original spare parts.
To operate the unit, use only original BINDER accessories or accessories from third-party suppliers
authorized by BINDER. The user is responsible for any risk caused by using unauthorized accessories.
CAUTION
Danger of overheating.
Damage to the unit.
∅Do NOT install the unit in unventilated recesses.
Ensure sufficient ventilation for dispersal of the heat.
Do not operate the refrigerated incubator KT in hazardous locations.
DANGER
Explosion hazard.
Danger of death.
∅Do NOT operate the unit in potentially explosive areas.
KEEP explosive dust or air-solvent mixtures AWAY from the unit.
The refrigerated incubator KT does not dispose of any measures of explosion protection.
DANGER
Explosion hazard.
Danger of death.
∅
Do NOT introduce any substance into the refrigerated incubator which is combustible or
explosive at working temperature.
∅NO explosive dust or air-solvent mixture in the inner chamber.
Any solvent contained in the charging material must not be explosive or inflammable. I.e., irrespective of
the solvent concentration in the steam room, NO explosive mixture with air must form. The temperature
inside the chamber must lie below the flash point or below the sublimation point of the charging material.
Familiarize yourself with the physical and chemical properties of the charging material, as well as the
contained moisture constituent and its behavior with the addition of heat energy.
Familiarize yourself with any potential health risks caused by the charging material, the contained
moisture constituent or by reaction products which may arise during the temperature process. Take
adequate measures to exclude such risks prior to putting the refrigerated incubator into operation.
DANGER
Electrical hazard.
Danger of death.
∅The unit must NOT become wet during operation or maintenance.
The refrigerated incubators were produced in accordance with VDE regulations and were routinely tested
in accordance to VDE 0411-1 (IEC 61010-1).

KT (E6) 09/2013 Page 15/135
CAUTION
The glass door and the inner chamber will become hot during operation.
Danger of burning.
∅Do NOT touch the glass door, the inner surfaces or the charging material during
operation.
1.6 Intended use
Refrigerated incubators KT are suitable for exact conditioning of harmless materials. Because of their
precise temperature accuracy these devices are especially useful for cultivation of microorganisms with a
narrow temperature optimum in a range of 4 °C / 39.2°F to 37 °C / 98.6°F. Main fields of application are
tests of long-term storage (e.g. at 4 °C / 39.2°F), refrigerated incubation between 20 °C / 68°F and 25 °C /
77°F and incubation at 37 °C / 98.6°F (also with additional introduction of heat) or with alternating
temperatures (e.g. 37 °C / 98.6°F and 4 °C / 39.2°F).
A mixture of any component of the charging material with air must NOT be explosive. The operating
temperature must lie below the flash point or below the sublimation point of the charging material.
Following the instructions in this operating manual and conducting regular maintenance work
(chap. 20.1) are part of the intended use.
The charging material shall not contain any corrosive ingredients that may damage the
machine components made of stainless steel, aluminum, and copper. Such ingredients
include in particular acids and halides. Any corrosive damage caused by such ingredients is
excluded from liability by BINDER GmbH.
WARNING: If customer should use a BINDER chamber running in non-supervised
continuous operation, we strongly recommend in case of inclusion of irrecoverable specimen
or samples to split such specimen or samples and store them in at least two chambers, if this
is feasible.

KT (E6) 09/2013 Page 16/135
2. Unit description
A high level of precision, reliability, and safety for all growth parameters ensures optimum incubation
conditions. Moreover, the KT refrigerated incubator is designed for maximum usability – even in
continuous operation year after year. It fulfills all technical and application-specific requirements arising in
experimentation such as in the areas of biotechnology, medicine, the nutrition industry, pharmaceutical
and cosmetics industries, botany, and zoology.
Two important temperature technologies have been combined to achieve perfect temperature control.
The Peltier refrigerating system, in conjunction with the APT.line™ preheating chamber technology,
satisfies the unique prerequisites for attaining highly-precise temperature control and particularly short
recovery times after opening the door.
The refrigerating system is distinguished by direct, precise, and rapid temperature conduction. Due to the
Peltier cooling, shocks are omitted which would occur during start and stop of conventional refrigeration
systems with a compressor.
The APT.line™ preheating chamber system ensures high level of spatial and time-based temperature
precision, thanks to the direct and distributed air circulation into the interior. This is especially important
for maintaining temperatures – especially with full chambers – and for rapid restoration of optimum growth
conditions after opening the door. The inner glass door ensures that the temperature remains constant
when observing the incubation process. The air turbine supports exact attainment and maintenance of the
desired temperature accuracy. The fan speed is digitally adjustable. The heating and refrigerating
systems are microprocessor regulated to a tenth of a degree. In addition, the refrigerated incubator
provides almost unlimited possibilities for adaptation to individual customer requirements based upon
extensive programming options and on the week program timer and real time clock of the controller.
All unit functions are easy and comfortable to use thanks to their clear arrangement. Major features are
easy cleaning of all unit parts and avoidance of undesired contamination.
The inner chamber, the pre-heating chamber and the interior side of the doors are all made of stainless
steel (material no. 1.4301 (V2A) in Germany, US equivalent 304). The housing is RAL 7035 powder-
coated. All corners and edges are also completely coated.
The refrigerated incubator KT comes equipped with an Ethernet interface for computer communication,
e.g. via the communication software APT-COM™ 3 DataControlSystem (option, chap. 19.1). For further
options, see chap. 23.5.
Temperature range with an ambient temperature of 25 °C / 77 °F: +4 °C / 23 °F up to +100 °C / 212 °F

KT (E6) 09/2013 Page 17/135
2.1 Unit overview
Instrument panel with
microprocessor controller
T4.12 and USB interface
Door handle
Unit door
Main power switch
Figure 3: Refrigerated incubator KT (example: model KT 115)
2.2 Instrument panel
5,7" controller display
Context-sensitive buttons
USB interface
Operating button
Pilot lamp: ready for operation
Figure 4: Instrument panel with microprocessor controller T4.12 and USB interface

KT (E6) 09/2013 Page 18/135
2.3 Unit rear
(1)
(3)
(4)
(5)
(6)
(7) (8)
Figure 5: Unit rear with position of options (example KT 115)
(1) DIN-socket for zero-voltage relay alarm outputs (option)
(2) (not used)
(3) DIN socket for analog output 4-20 mA (option)
(4) DIN-socket for zero-voltage relay control outputs (option)
(5) RS 422 interface for computer communication (option)
(6) Ethernet interface for computer communication with MAC address (standard equipment, not with
optional RS 422 interface)
(7) Peltier fan grid
(8) Socket for IEC connector plug with power cable

KT (E6) 09/2013 Page 19/135
3. Completeness of delivery, transportation, storage, and location
of installation
3.1 Unpacking, and checking equipment and completeness of delivery
After unpacking, please check the unit and its optional accessories, if any, based on the delivery receipt
for completeness and for transportation damage. Inform the carrier immediately if transportation damage
has occurred.
The final tests of the manufacturer may have caused traces of the shelves on the inner surfaces. This has
no impact on the function and performance of the unit.
Please remove any transportation protection devices and adhesives in/on the unit and on the doors and
remove the operating manuals and accessory equipment.
CAUTION
Sliding or tilting of the unit.
Damage to the unit.
Risk of injury by lifting heavy loads.
Do NOT lift or transport the unit using the door or the door handle.
Lift the unit from the pallet at the four lower corners with the aid of four people.
If you need to return the unit, please use the original packing and observe the guidelines for safe lifting
and transportation (chap. 3.2).
For disposal of the transport packing, see chap. 21.1.
Note on second-hand units (Ex-Demo-Units):
Second-hand units are units that were used for a short time for tests or exhibitions. They are thoroughly
tested before resale. BINDER ensures that the chamber is technically sound and will work flawlessly.
Second-hand units are marked with a sticker on the unit door. Please remove the sticker before
commissioning the unit.
3.2 Guidelines for safe lifting and transportation
After operation, please observe the guidelines for temporary decommissioning (chap. 21.2).
CAUTION
Sliding or tilting of the unit.
Damage to the unit.
Risk of injury by lifting heavy loads.
Transport the unit in its original packaging only.
For moving or shipping, secure the unit with transport straps.
∅Do NOT lift or transport the unit using the door or the door handle.
Lift the unit at the four lower corners with the aid of 4 people and place it on a rolling
pallet.

KT (E6) 09/2013 Page 20/135
Permissible ambient temperature range during transport: 10 °C / 14°F to +60 °C / 140°F.
You can order transport packing for moving or shipping purposes from BINDER service.
3.3 Storage
Intermediate storage of the unit is possible in a closed and dry room. Observe the guidelines for
temporary decommissioning (chap. 21.2).
•Permissible ambient temperature range during storage: -10 °C / 14°F to +60 °C / 140°F.
•Permissible ambient humidity: max. 70 % r.H., non-condensing
When after storage in a cold location you transfer the unit to its warmer installation site, condensation
may form. Before start-up, wait at least one hour until the chamber has attained ambient temperature and
is completely dry.
3.4 Location of installation and ambient conditions
Set up the refrigerated incubator KT on a flat, even surface, free from vibration, in a well-ventilated, dry
location and align it using a spirit level. The site of installation must be capable of supporting the unit’s
weight (see technical data, chap. 23.4). The chambers are designed for setting up inside a building
(indoor use).
CAUTION
Danger of overheating.
Damage to the unit.
Do NOT set up units in non-ventilated recesses.
Ensure sufficient ventilation for dispersal of the heat.
•Permissible ambient temperature range during operation: +18 °C / 64.4°F to +25 °C / 77 °F. At
elevated ambient temperature values, fluctuations in temperature can occur.
The ambient temperature should not be substantially higher than the indicated ambient
temperature of +25 °C / 77°F to which the specified technical data relates. Deviations from the
indicated data are possible for other ambient conditions.
With each degree of ambient temperature > +25 °C / 77°F, the refrigeration power decreases
by 1.5 K.
•Permissible ambient humidity: 70 % r.H. max., non-condensing.
When operating the chamber at temperature set-points below ambient temperature, high ambient
humidity may lead to condensation on the unit.
•Installation height: max. 2000 m / 6.6 ft. above sea level.
When placing several units of the same size side by side, maintain a minimum distance of 250 mm / 9.84
in between each unit. Wall distances (minimum distances): rear 100 mm / 3.94 in, sides 240 mm / 9.45 in.
Spacing above the unit of at least 100 mm / 3.94 in must also be maintained
Two devices up to size 115l can be piled on top of each other. For this purpose, place rubber pads under
all four feet of the upper unit to prevent the device from slipping.
CAUTION
Sliding or tilting of the upper unit.
Damage to the units.
When stacking, place rubber pads under all four feet of the upper unit.